Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,528,973 | T→C | 10.9% | E401G (GAG→GGG) | ligA ← | DNA ligase, NAD(+)‑dependent |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,528,973 | 0 | T | C | 10.9% | 298.6 / 4.3 | 129 | E401G (GAG→GGG) | ligA | DNA ligase, NAD(+)‑dependent |
Reads supporting (aligned to +/- strand): ref base T (46/62); new base C (12/5); total (59/70) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.82e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 129 total aligned reads displayed.
ATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTA > NC_000913/2528701‑2529243 | aTTTTGGCCCCTGACGGTCCTGTCCGGTTAGTTTGCCTGCGGTGAGTTTGAACAGAGCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCa < 1:527350/275‑1 (MQ=255) aTTTTGGCCCCATCCGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCa < 1:819354/275‑1 (MQ=255) aTTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCa < 1:776171/275‑1 (MQ=255) tACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCCACTCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACCCCCAACGTCCGCACCCCACACCGGACAATGCGTCGGGAAAACACCCCCACGGGTATCTT‑c > 2:23132/1‑274 (MQ=255) tCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGCCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACCTCAGAACCACATACCGGACCATGCCTCCGGCATACAACCTCCAGGGTATCTT‑CCCGGCGTCCac > 2:1031431/1‑274 (MQ=255) ccGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCAGTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGa < 1:676203/275‑1 (MQ=255) ttagttgtgcTGCGGTGAGTTTGAACAGATCTGCCGGATTCTGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGc < 2:592556/266‑1 (MQ=255) tgaGTTGGCGTGGGGTGAGGTTGGACGGATCGTCCGCGGTATGGTCTTGTTCGTTTTCAACCGGCGGTTCGATGATTTTGTCACCCATTCGCTCAGCACCCAACGCACG‑GCGGGAAAGAAAGTGTTTCAGACACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGc < 2:724696/273‑1 (MQ=255) tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACCCGCTCCACGTCAGAACCACATACCGGACAATGCGTCGGGAAAACAACCCCCCGGGTATCTT‑CCGGGGGTTCAGAAAg > 2:26168/1‑274 (MQ=255) tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGaa > 1:102800/1‑272 (MQ=255) tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAg > 1:459583/1‑274 (MQ=255) tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAg > 1:970218/1‑274 (MQ=255) tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAACCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCCGAACCACATACCGGACAATGCGTCGGGAATACAACCCACCGGATACCTC‑CCGGGCCTTccacaag > 2:743240/1‑268 (MQ=255) ccggttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTGTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCa < 2:227824/271‑1 (MQ=255) cAGTTTGCCTGCGGGGAGGTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCa < 1:646806/275‑1 (MQ=255) cAGTTGGTCTGCGGGGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCa < 2:812743/275‑1 (MQ=255) tttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATCCAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCacga > 1:122897/1‑275 (MQ=255) tttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCa < 2:102800/272‑1 (MQ=255) ttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGCAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGCAAATGCGTCGGGAATACCACCCCACGGGTAGCTT‑CCGGGCGTTCAGAAAGCacgac > 2:499653/1‑275 (MQ=255) gTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACAGCTTCACC‑TTCCACACGCCCAACGTCAGAACCACATACCGGACAAGGCGTCGGGAATACAACCTAACGGGGACCTT‑CCGGGCGTTCAGAAAGCACGACGTTAacaa > 1:994295/1‑272 (MQ=255) tGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACCTCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCggg < 1:191985/275‑1 (MQ=255) atgggtataagaaacaggTATGGACATATTCTTTTTCAACCAGCTGAGCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCggg < 1:9986/258‑1 (MQ=255) gATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCACATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACCCGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATCCAACCCCCAGGGTATCAT‑CCGGGCGTTCAGAAAACCCCACGTTACCCCCCTACGGGATCCCg > 2:755421/1‑275 (MQ=255) gATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCACAGTCAGAACCACATACCGGCCACTGCGTCCGGAATACAACCTCACGGGTATCTT‑CCCGGCGTTCAGCAAGCACGACGTTAAACACCTGCGGGATCACg > 1:271058/1‑275 (MQ=255) gATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACg > 2:708186/1‑275 (MQ=255) gTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTAAAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCCCACGGGTATATT‑CCGGGCGTTCAGAAAGCACGCCGTTAACCACCTGCGGGACTCCGGCGCCcgcgcg > 1:487090/1‑275 (MQ=255) tATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTTCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCCCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg > 2:211704/1‑275 (MQ=255) tATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGACCCACATACCGGACAATGCGTCGGGAATACACCCTCACGGGTACCTT‑CCGGGCGTTCAGAAACCACGACGTTAACCAACTGCGGGATAACGTCGCAAGCgcgg > 1:439392/1‑275 (MQ=255) tATGGACATATTCTTGTTCAACCAGCTGATGGATGAGTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg < 2:915815/275‑1 (MQ=255) aCATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAAt < 2:970217/275‑1 (MQ=255) aCATATGCTGTGTCAGCCAGCTGATCGATGATTTTTTCCCCCCTTCCGTCAACCTCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGTTGCGCACCGCAAATCAGGCCACCGGTACCGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAAt < 1:1005532/275‑1 (MQ=255) tatTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTACCCACCTGCGGGATAACGTCTCCAGCGCGGCGCATccc > 1:245523/1‑273 (MQ=255) atTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACTTTAACCACCTGACGGATCACGTCGCCAGAGCGGCGAATcccc > 2:743512/1‑275 (MQ=255) actCTTTTTCAACCGGCCGATCGATGATTTTTTGGCCCATTCCGTCAACATCCATCGCACG‑GCTGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAACTCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcacc < 2:394860/273‑1 (MQ=255) ttCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcacc > 1:727727/1‑274 (MQ=255) ttCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcaca > 1:625494/1‑273 (MQ=255) ccAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAACCAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCa < 1:88721/275‑1 (MQ=255) ccAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCa < 2:459583/275‑1 (MQ=255) aGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAAt > 1:203523/1‑275 (MQ=255) cTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCCTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCAACACTTTATCGCCAATGc > 1:481830/1‑275 (MQ=255) tGAGCGATGATTGTGTCGCCCATTCCGTCAACATCCATCGCGCG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCCCCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg < 2:89138/275‑1 (MQ=255) atTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACACCCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCaa > 1:426389/1‑274 (MQ=255) gTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTC‑CCGGGCGTTCCGAAAGAACGACGTTAACCACCCGAGGGATTCCGTCGCCAGCGCGGCCAATCCCCCCTTTCTGCCCAATGCGTAAACCCAAACGt > 2:866504/1‑274 (MQ=255) gCCCCTTCCGTCAAAATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGACCACCGGTACAACGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACAACATACCGGACAATACGTCGGGAACCCCACCTCCCGAGTATCGT‑CAGAGCTATCGGAAAGCACGACGGTAGTCACCTGCGGGATCACGTCGCCAGCGAGTCGAAACACCACTCTATCACACATCCGTAACCAAAGACggtaaa > 2:990028/1‑270 (MQ=255) aTTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACACAGTTATACCCAATGCGTAAACCAAGCCGGTCGAGTTc > 1:153226/1‑275 (MQ=255) gtcaagaaccacgcaccg‑gcggGAACCAAAGTGTTTCAGCGCCCCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGACCCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCg < 1:131830/259‑1 (MQ=255) gcaCATCCATC‑CACGTGCGAGTAACAAAGTATTTCAGCGACCTTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGTACAATGCGGCGGGAATACGACCGCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCAGCCGCa < 2:770000/273‑1 (MQ=255) caccacccatcGCACG‑GCGGGAAACAAAGTGTTTCAGGGACTCTTTCCGCTGGGCACCCAAAATCAGGCCACCGTTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 2:422654/269‑1 (MQ=255) cacacgccaacGCACG‑GCGGGAAACAAAGGGTTTCAGCGACTCTTTACGCTGCCCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCCCC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 2:750112/269‑1 (MQ=255) cAACCTCCAGCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCCCCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACCCGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCTCGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 1:805804/275‑1 (MQ=255) cAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGGCACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTACACACGCTCAACGTCATAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCTGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 1:462903/275‑1 (MQ=255) cAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 1:398417/275‑1 (MQ=255) cAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGGACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 1:743512/275‑1 (MQ=255) aaaCACCCATCGCACG‑GCGGGAAAGAAATTGTTTCAGCGACTCTTTACGCGGCGCACCGCAAATCAGGCCACCGGTACAGCAGGCAACCGCTTCACC‑TGCCACACGCTCAACGTCAGAACCACATCCCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 2:19615/274‑1 (MQ=255) atcGCAGC‑GGGGGAGACACAGTGTGTCAGCGACTCTTTCCCGTGCCCACCGCAAATCAGGCCACCGGTACGACGGTCAACCGGTTCACC‑TTCCAAACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTaa < 2:452226/275‑1 (MQ=255) cg‑gcggGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCACTGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTg > 1:785800/1‑274 (MQ=255) cg‑gcggGAAACAAAGTGTTTCAGCGAATCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCCCACGGGTATCTT‑CCCGGCGTTCAGAAAGCACGACGTTAACCCCCTGCGGGATCACGTCCCCAGCGCGCGGAATCACCACTTTATCGCCAATGCGAAAACAAAAACGTTGGATTTCAACCCCA‑TTttgtcactgtt > 2:622108/1‑267 (MQ=255) cggGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTCAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGt > 1:729100/1‑274 (MQ=255) aGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGc < 1:708186/275‑1 (MQ=255) aGTGTTCCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACAGACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAg < 1:499653/274‑1 (MQ=255) aGTGGTGCAGCGACACTTTGCGCTGCGGCCCGCCATCCAGGCCGACGGTCCGGCGTGAAACCGCTTCCCC‑TTCCACCCGCTCAACGTCCGAACCACGTACGGGACAATGGGGCGGGAATACAACCTCACGGGTATCGT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCCCGTCGCCAGCGCGGCGAATCACCACTTTCGCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAg < 2:825101/274‑1 (MQ=255) ccgcgatgctTTACGCGGCGCCCCGCAAATCAGGCCACCGGTACAGCGGCCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACAACAGAACGGCCAATGCGTCGGGAATACAACCTCACGGTTATCAT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCCCGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCTCCAGCACGCCt < 2:703640/266‑1 (MQ=255) cAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCt < 1:26168/274‑1 (MQ=255) aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACCGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACACCCCCACGGGTATCTT‑CCGGGCGATCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGACAGCGCGGCGAATCACCACTTTATCGCCACTGCGTAAACCAAGACGTTCGCTTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACa > 1:225846/1‑275 (MQ=255) aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAAcc > 2:656953/1‑274 (MQ=255) aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCCTCGGGAATACAACCCCACGGGTATCTT‑CCGGGCGTCCAGAAAGCCCGAAGTTAACGACCTGCGGGAGCACGCCGCCAGCGAGGCAAATCACCACTTTATCGCCAATGCGTCAACCAAGACGTTCGATTTCAACCGCA‑TTCTGCATGTTTCCTTGCCTCACCAGCACGCCTCCAACa > 2:39700/1‑275 (MQ=255) ttACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCGGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGa < 1:23132/274‑1 (MQ=255) tACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGAGCGTTCAGAAAGCCAGACGTTAACCACCTGCGGGATCACGTCGCCCTCGCGGCGAATCACCACTTTATCACAACTGCGTCACCCAAGACGTTCGACTTCATCCGCA‑TTGTGTAAGGATACGTGCGTCACAAGCACGCCAGCACCATgcgca > 2:151251/1‑271 (MQ=255) cgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGAGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTc > 2:478458/1‑274 (MQ=255) gcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACGTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACAAGCACGCCTGCAAACTGGACAGGTTcc > 1:357302/1‑273 (MQ=255) aaaTCAGGCCCCCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACTCTCAACGTCAGACCCACATACCGGACAATGCGTCCGGAATACAACCTCACGGGTATCTT‑CCGGGCTTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCa < 1:295753/275‑1 (MQ=255) aagcAGGCCACCGGGAACGCGGGCAACCCCTTCACC‑TTCCACACGCTCCACGACAGAGCCACAGACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCATAAAGCACGACGTGAACCACCGGCGGGATAACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCACTGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGGGTAAGGTTGCGTTACTCACCAGCACGCTTGCAACATGGACAGGTTCCAGACGCGCaa < 2:625494/272‑1 (MQ=255) gCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCCGGGCGTTCAGCAAACACGCCGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTCCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAACACCCCTGCAACATGGACCGGTTCCAGCCGCGCAACAGGc > 1:1001751/1‑275 (MQ=255) cGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGAAACATGGACAGGTTCCAGACCGCCAACAGGCGTAATCGCGCCAGt > 2:977659/1‑275 (MQ=255) aaCCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGcc > 1:422557/1‑275 (MQ=255) aaCCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATAACGGACAATGCGTCGGGAATACAACCACCAGGGTATCTT‑CCGAGAGGTCAGAAAGCACGACGTTAACCACCTGATGGCTCACGTACCAAGCTCGGCGAATCACGACCTGACCGCCAATGCGTAAACAAAGACCATCGAACTCCGCCGCACATGT‑TTGGCTTGAGTTACTCACCAGCACGCCTGACACTAGGACAGGTTCCACAAGCCGAACATGCGTactgcccccacgaacac > 2:808133/1‑259 (MQ=255) tCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTGTCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGCTCGATTTCACCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAAACTGGACAGGTTCCAGAAGCGCACCAGGCGTAATAGCGCCAGTACGCCCACCCTGAAAATc > 2:806484/1‑275 (MQ=255) cgcgCAACGGCAAGACCACAAACCGGACAAGGCCGCGGGAATACACCCCCACGGGGACCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTc < 2:439392/275‑1 (MQ=255) aaCGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGca < 1:195562/275‑1 (MQ=255) aaCGTCAGAACCACATACCGGACAATGCGGCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGca < 1:798620/275‑1 (MQ=255) aCGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATc > 2:189267/1‑161 (MQ=255) gTCAGACCCACAAACCGGACAATGCGTCGGGAATACAACCGCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGTCAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACaa < 2:395474/275‑1 (MQ=255) cAGAACCCCAAACCGGGCAATGCGGCGGGAATAACACCCCACGGGTATCTT‑CCGGGCGTTACGAAAGCACGACCTTAACCACCTGCGGGATCCCGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAg < 2:481830/275‑1 (MQ=255) cAGAACCACATACCGGACAATGCGTCGGGAATACACCCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 1:189267/161‑1 (MQ=255) aGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAgg < 1:211704/275‑1 (MQ=255) cccgtacctgcaaaTGCCTCGGGAATACAACCCCACGGGCATCTT‑CCGGGTGTTCATAAAGCACGACTTTAACCACCGTAGGGATCACGTCGCCAGCGCGGCTAATCACCACTTTCTCGCCAATTCGTAAACCAAGCCGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCCCGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATc < 1:436710/263‑1 (MQ=255) ccagaccgaaaacggcctCGTGAATACAACCTCACGGGTATCTT‑CAGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGGTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATc < 1:549720/257‑1 (MQ=255) caTACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGtt > 1:78485/1‑139 (MQ=255) ccGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGAt < 2:78485/139‑1 (MQ=255) ccGAACAATGCTGCGGGAATACAACCTCCCGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCCGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTcc < 1:838431/275‑1 (MQ=255) aaTGGGTCGGAAATACAACCCCACGGGGATCTT‑CCGGGCGGTCAGAAAGACCGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTAGCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGcc < 2:785800/275‑1 (MQ=255) aaTGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGAGTTCATCCACA‑TTGTGTAAGGTTGAGTTACTCACCAGCACGCCTGCAACATGGACAGGTTACAGCCGCGCAACAGGCGTAATATCGCCAGTACCCCCACCCTCAAACACGACGTCCCGCCCAAAGGTCAACTGCTCCTgaccc > 2:261675/1‑271 (MQ=255) tgggTCGGGAAGCCAACCCACCGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTACCCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCgg < 1:410177/272‑1 (MQ=255) cGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATc > 1:358716/1‑132 (MQ=255) aaTACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa < 2:358716/132‑1 (MQ=255) agaCAACCTCACGGGTATCTT‑CCGGACGTTCAGAAAGAACGACGTTAACCACCGGCGCGATCACGTCGCCCGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAGGACGTTCTATTTCATCCTCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa < 1:452876/272‑1 (MQ=255) aTACAACCTCACGGGTATCTT‑CCGGGCTTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa < 1:977660/274‑1 (MQ=255) aTACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa < 2:203523/274‑1 (MQ=255) tACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCAACCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCCACAGGCGTAATCGCCCCAGTACGCCCAACCTGAACCTCGACGTCACGCCCAAAGGTCATCTGCCCCTGCGCCGGGACTTTacaca > 1:107625/1‑274 (MQ=255) aaCCTAACGCGTATCTT‑CCGTGCGGTCAGAACGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCACGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGAGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTa < 1:1029801/275‑1 (MQ=255) | ATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACG‑GCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACC‑TTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTT‑CCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCA‑TTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTA > NC_000913/2528701‑2529243 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |