Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,528,973 T→C 10.9% E401G (GAG→GGG)  ligA ← DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,528,9730TC10.9% 298.6 / 4.3 129E401G (GAG→GGG) ligADNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base T (46/62);  new base C (12/5);  total (59/70)
Fisher's exact test for biased strand distribution p-value = 3.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

ATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTA  >  NC_000913/2528701‑2529243
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                
aTTTTGGCCCCTGACGGTCCTGTCCGGTTAGTTTGCCTGCGGTGAGTTTGAACAGAGCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCa                                                                                                                                                                                                                                                                                <  1:527350/275‑1 (MQ=255)
aTTTTGGCCCCATCCGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCa                                                                                                                                                                                                                                                                                <  1:819354/275‑1 (MQ=255)
aTTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCa                                                                                                                                                                                                                                                                                <  1:776171/275‑1 (MQ=255)
            tACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCCACTCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACCCCCAACGTCCGCACCCCACACCGGACAATGCGTCGGGAAAACACCCCCACGGGTATCTTc                                                                                                                                                                                                                                                                    >  2:23132/1‑274 (MQ=255)
                      tCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGCCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACCTCAGAACCACATACCGGACCATGCCTCCGGCATACAACCTCCAGGGTATCTTCCCGGCGTCCac                                                                                                                                                                                                                                                         >  2:1031431/1‑274 (MQ=255)
                       ccGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCAGTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGa                                                                                                                                                                                                                                                        <  1:676203/275‑1 (MQ=255)
                           ttagttgtgcTGCGGTGAGTTTGAACAGATCTGCCGGATTCTGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGc                                                                                                                                                                                                                                                    <  2:592556/266‑1 (MQ=255)
                           tgaGTTGGCGTGGGGTGAGGTTGGACGGATCGTCCGCGGTATGGTCTTGTTCGTTTTCAACCGGCGGTTCGATGATTTTGTCACCCATTCGCTCAGCACCCAACGCACGGCGGGAAAGAAAGTGTTTCAGACACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGc                                                                                                                                                                                                                                                    <  2:724696/273‑1 (MQ=255)
                           tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACCCGCTCCACGTCAGAACCACATACCGGACAATGCGTCGGGAAAACAACCCCCCGGGTATCTTCCGGGGGTTCAGAAAg                                                                                                                                                                                                                                                     >  2:26168/1‑274 (MQ=255)
                           tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGaa                                                                                                                                                                                                                                                       >  1:102800/1‑272 (MQ=255)
                           tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAg                                                                                                                                                                                                                                                     >  1:459583/1‑274 (MQ=255)
                           tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAg                                                                                                                                                                                                                                                     >  1:970218/1‑274 (MQ=255)
                           tCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAACCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCCGAACCACATACCGGACAATGCGTCGGGAATACAACCCACCGGATACCTCCCGGGCCTTccacaag                                                                                                                                                                                                                                                     >  2:743240/1‑268 (MQ=255)
                            ccggttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTGTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                                                                                                                                                                                                   <  2:227824/271‑1 (MQ=255)
                            cAGTTTGCCTGCGGGGAGGTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                                                                                                                                                                                                   <  1:646806/275‑1 (MQ=255)
                            cAGTTGGTCTGCGGGGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                                                                                                                                                                                                   <  2:812743/275‑1 (MQ=255)
                               tttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATCCAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCacga                                                                                                                                                                                                                                                >  1:122897/1‑275 (MQ=255)
                               tttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                                                                                                                                                                                                   <  2:102800/272‑1 (MQ=255)
                                ttGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGCAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGCAAATGCGTCGGGAATACCACCCCACGGGTAGCTTCCGGGCGTTCAGAAAGCacgac                                                                                                                                                                                                                                               >  2:499653/1‑275 (MQ=255)
                                         gTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACAGCTTCACCTTCCACACGCCCAACGTCAGAACCACATACCGGACAAGGCGTCGGGAATACAACCTAACGGGGACCTTCCGGGCGTTCAGAAAGCACGACGTTAacaa                                                                                                                                                                                                                                       >  1:994295/1‑272 (MQ=255)
                                                tGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACCTCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCggg                                                                                                                                                                                                                               <  1:191985/275‑1 (MQ=255)
                                                atgggtataagaaacaggTATGGACATATTCTTTTTCAACCAGCTGAGCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCggg                                                                                                                                                                                                                               <  1:9986/258‑1 (MQ=255)
                                                      gATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCACATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACCCGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATCCAACCCCCAGGGTATCATCCGGGCGTTCAGAAAACCCCACGTTACCCCCCTACGGGATCCCg                                                                                                                                                                                                                         >  2:755421/1‑275 (MQ=255)
                                                      gATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCACAGTCAGAACCACATACCGGCCACTGCGTCCGGAATACAACCTCACGGGTATCTTCCCGGCGTTCAGCAAGCACGACGTTAAACACCTGCGGGATCACg                                                                                                                                                                                                                         >  1:271058/1‑275 (MQ=255)
                                                      gATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACg                                                                                                                                                                                                                         >  2:708186/1‑275 (MQ=255)
                                                                 gTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTAAAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCCCACGGGTATATTCCGGGCGTTCAGAAAGCACGCCGTTAACCACCTGCGGGACTCCGGCGCCcgcgcg                                                                                                                                                                                                              >  1:487090/1‑275 (MQ=255)
                                                                  tATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTTCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCCCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg                                                                                                                                                                                                             >  2:211704/1‑275 (MQ=255)
                                                                  tATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGACCCACATACCGGACAATGCGTCGGGAATACACCCTCACGGGTACCTTCCGGGCGTTCAGAAACCACGACGTTAACCAACTGCGGGATAACGTCGCAAGCgcgg                                                                                                                                                                                                             >  1:439392/1‑275 (MQ=255)
                                                                  tATGGACATATTCTTGTTCAACCAGCTGATGGATGAGTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg                                                                                                                                                                                                             <  2:915815/275‑1 (MQ=255)
                                                                       aCATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAAt                                                                                                                                                                                                        <  2:970217/275‑1 (MQ=255)
                                                                       aCATATGCTGTGTCAGCCAGCTGATCGATGATTTTTTCCCCCCTTCCGTCAACCTCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGTTGCGCACCGCAAATCAGGCCACCGGTACCGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAAt                                                                                                                                                                                                        <  1:1005532/275‑1 (MQ=255)
                                                                          tatTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTACCCACCTGCGGGATAACGTCTCCAGCGCGGCGCATccc                                                                                                                                                                                                     >  1:245523/1‑273 (MQ=255)
                                                                           atTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACTTTAACCACCTGACGGATCACGTCGCCAGAGCGGCGAATcccc                                                                                                                                                                                                    >  2:743512/1‑275 (MQ=255)
                                                                           actCTTTTTCAACCGGCCGATCGATGATTTTTTGGCCCATTCCGTCAACATCCATCGCACGGCTGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAACTCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcacc                                                                                                                                                                                                    <  2:394860/273‑1 (MQ=255)
                                                                            ttCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcacc                                                                                                                                                                                                    >  1:727727/1‑274 (MQ=255)
                                                                            ttCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcaca                                                                                                                                                                                                    >  1:625494/1‑273 (MQ=255)
                                                                                       ccAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAACCAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCa                                                                                                                                                                                        <  1:88721/275‑1 (MQ=255)
                                                                                       ccAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCa                                                                                                                                                                                        <  2:459583/275‑1 (MQ=255)
                                                                                         aGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAAt                                                                                                                                                                                      >  1:203523/1‑275 (MQ=255)
                                                                                           cTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCCTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCAACACTTTATCGCCAATGc                                                                                                                                                                                    >  1:481830/1‑275 (MQ=255)
                                                                                            tGAGCGATGATTGTGTCGCCCATTCCGTCAACATCCATCGCGCGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCCCCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg                                                                                                                                                                                   <  2:89138/275‑1 (MQ=255)
                                                                                                     atTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACACCCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCaa                                                                                                                                                                           >  1:426389/1‑274 (MQ=255)
                                                                                                          gTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTCCCGGGCGTTCCGAAAGAACGACGTTAACCACCCGAGGGATTCCGTCGCCAGCGCGGCCAATCCCCCCTTTCTGCCCAATGCGTAAACCCAAACGt                                                                                                                                                                      >  2:866504/1‑274 (MQ=255)
                                                                                                             gCCCCTTCCGTCAAAATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGACCACCGGTACAACGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACAACATACCGGACAATACGTCGGGAACCCCACCTCCCGAGTATCGTCAGAGCTATCGGAAAGCACGACGGTAGTCACCTGCGGGATCACGTCGCCAGCGAGTCGAAACACCACTCTATCACACATCCGTAACCAAAGACggtaaa                                                                                                                                                                  >  2:990028/1‑270 (MQ=255)
                                                                                                                 aTTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACACAGTTATACCCAATGCGTAAACCAAGCCGGTCGAGTTc                                                                                                                                                              >  1:153226/1‑275 (MQ=255)
                                                                                                                      gtcaagaaccacgcaccggcggGAACCAAAGTGTTTCAGCGCCCCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGACCCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCg                                                                                                                                                         <  1:131830/259‑1 (MQ=255)
                                                                                                                        gcaCATCCATCCACGTGCGAGTAACAAAGTATTTCAGCGACCTTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGTACAATGCGGCGGGAATACGACCGCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCAGCCGCa                                                                                                                                                       <  2:770000/273‑1 (MQ=255)
                                                                                                                        caccacccatcGCACGGCGGGAAACAAAGTGTTTCAGGGACTCTTTCCGCTGGGCACCCAAAATCAGGCCACCGTTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  2:422654/269‑1 (MQ=255)
                                                                                                                        cacacgccaacGCACGGCGGGAAACAAAGGGTTTCAGCGACTCTTTACGCTGCCCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCCCCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  2:750112/269‑1 (MQ=255)
                                                                                                                        cAACCTCCAGCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCCCCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACCCGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCTCGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  1:805804/275‑1 (MQ=255)
                                                                                                                        cAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGGCACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTACACACGCTCAACGTCATAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCTGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  1:462903/275‑1 (MQ=255)
                                                                                                                        cAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  1:398417/275‑1 (MQ=255)
                                                                                                                        cAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGGACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  1:743512/275‑1 (MQ=255)
                                                                                                                        aaaCACCCATCGCACGGCGGGAAAGAAATTGTTTCAGCGACTCTTTACGCGGCGCACCGCAAATCAGGCCACCGGTACAGCAGGCAACCGCTTCACCTGCCACACGCTCAACGTCAGAACCACATCCCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  2:19615/274‑1 (MQ=255)
                                                                                                                                atcGCAGCGGGGGAGACACAGTGTGTCAGCGACTCTTTCCCGTGCCCACCGCAAATCAGGCCACCGGTACGACGGTCAACCGGTTCACCTTCCAAACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTaa                                                                                                                                              <  2:452226/275‑1 (MQ=255)
                                                                                                                                      cggcggGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCACTGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTg                                                                                                                                         >  1:785800/1‑274 (MQ=255)
                                                                                                                                      cggcggGAAACAAAGTGTTTCAGCGAATCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCCCACGGGTATCTTCCCGGCGTTCAGAAAGCACGACGTTAACCCCCTGCGGGATCACGTCCCCAGCGCGCGGAATCACCACTTTATCGCCAATGCGAAAACAAAAACGTTGGATTTCAACCCCATTttgtcactgtt                                                                                                                                         >  2:622108/1‑267 (MQ=255)
                                                                                                                                          cggGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTCAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGt                                                                                                                                      >  1:729100/1‑274 (MQ=255)
                                                                                                                                                    aGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGc                                                                                                                           <  1:708186/275‑1 (MQ=255)
                                                                                                                                                    aGTGTTCCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACAGACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg                                                                                                                            <  1:499653/274‑1 (MQ=255)
                                                                                                                                                    aGTGGTGCAGCGACACTTTGCGCTGCGGCCCGCCATCCAGGCCGACGGTCCGGCGTGAAACCGCTTCCCCTTCCACCCGCTCAACGTCCGAACCACGTACGGGACAATGGGGCGGGAATACAACCTCACGGGTATCGTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCCCGTCGCCAGCGCGGCGAATCACCACTTTCGCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAg                                                                                                                            <  2:825101/274‑1 (MQ=255)
                                                                                                                                                           ccgcgatgctTTACGCGGCGCCCCGCAAATCAGGCCACCGGTACAGCGGCCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACAACAGAACGGCCAATGCGTCGGGAATACAACCTCACGGTTATCATCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCCCGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCTCCAGCACGCCt                                                                                                                     <  2:703640/266‑1 (MQ=255)
                                                                                                                                                           cAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCt                                                                                                                     <  1:26168/274‑1 (MQ=255)
                                                                                                                                                                aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACCGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACACCCCCACGGGTATCTTCCGGGCGATCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGACAGCGCGGCGAATCACCACTTTATCGCCACTGCGTAAACCAAGACGTTCGCTTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACa                                                                                                               >  1:225846/1‑275 (MQ=255)
                                                                                                                                                                aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAAcc                                                                                                               >  2:656953/1‑274 (MQ=255)
                                                                                                                                                                aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCCTCGGGAATACAACCCCACGGGTATCTTCCGGGCGTCCAGAAAGCCCGAAGTTAACGACCTGCGGGAGCACGCCGCCAGCGAGGCAAATCACCACTTTATCGCCAATGCGTCAACCAAGACGTTCGATTTCAACCGCATTCTGCATGTTTCCTTGCCTCACCAGCACGCCTCCAACa                                                                                                               >  2:39700/1‑275 (MQ=255)
                                                                                                                                                                     ttACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCGGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGa                                                                                                           <  1:23132/274‑1 (MQ=255)
                                                                                                                                                                      tACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGAGCGTTCAGAAAGCCAGACGTTAACCACCTGCGGGATCACGTCGCCCTCGCGGCGAATCACCACTTTATCACAACTGCGTCACCCAAGACGTTCGACTTCATCCGCATTGTGTAAGGATACGTGCGTCACAAGCACGCCAGCACCATgcgca                                                                                                         >  2:151251/1‑271 (MQ=255)
                                                                                                                                                                             cgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGAGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTc                                                                                                    >  2:478458/1‑274 (MQ=255)
                                                                                                                                                                              gcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACGTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACAAGCACGCCTGCAAACTGGACAGGTTcc                                                                                                   >  1:357302/1‑273 (MQ=255)
                                                                                                                                                                                     aaaTCAGGCCCCCGGTACAGCGGGCAACCGCTTCACCTTCCACACTCTCAACGTCAGACCCACATACCGGACAATGCGTCCGGAATACAACCTCACGGGTATCTTCCGGGCTTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCa                                                                                          <  1:295753/275‑1 (MQ=255)
                                                                                                                                                                                      aagcAGGCCACCGGGAACGCGGGCAACCCCTTCACCTTCCACACGCTCCACGACAGAGCCACAGACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCATAAAGCACGACGTGAACCACCGGCGGGATAACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCACTGCGTAAACCAAGACGTTCGATTTCATCCGCATTGGGTAAGGTTGCGTTACTCACCAGCACGCTTGCAACATGGACAGGTTCCAGACGCGCaa                                                                                         <  2:625494/272‑1 (MQ=255)
                                                                                                                                                                                            gCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCCGGGCGTTCAGCAAACACGCCGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTCCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAACACCCCTGCAACATGGACCGGTTCCAGCCGCGCAACAGGc                                                                                    >  1:1001751/1‑275 (MQ=255)
                                                                                                                                                                                                         cGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGAAACATGGACAGGTTCCAGACCGCCAACAGGCGTAATCGCGCCAGt                                                                      >  2:977659/1‑275 (MQ=255)
                                                                                                                                                                                                              aaCCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGcc                                                                 >  1:422557/1‑275 (MQ=255)
                                                                                                                                                                                                              aaCCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATAACGGACAATGCGTCGGGAATACAACCACCAGGGTATCTTCCGAGAGGTCAGAAAGCACGACGTTAACCACCTGATGGCTCACGTACCAAGCTCGGCGAATCACGACCTGACCGCCAATGCGTAAACAAAGACCATCGAACTCCGCCGCACATGTTTGGCTTGAGTTACTCACCAGCACGCCTGACACTAGGACAGGTTCCACAAGCCGAACATGCGTactgcccccacgaacac                                                                 >  2:808133/1‑259 (MQ=255)
                                                                                                                                                                                                                            tCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTGTCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGCTCGATTTCACCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAAACTGGACAGGTTCCAGAAGCGCACCAGGCGTAATAGCGCCAGTACGCCCACCCTGAAAATc                                                    >  2:806484/1‑275 (MQ=255)
                                                                                                                                                                                                                                  cgcgCAACGGCAAGACCACAAACCGGACAAGGCCGCGGGAATACACCCCCACGGGGACCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTc                                              <  2:439392/275‑1 (MQ=255)
                                                                                                                                                                                                                                       aaCGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGca                                         <  1:195562/275‑1 (MQ=255)
                                                                                                                                                                                                                                       aaCGTCAGAACCACATACCGGACAATGCGGCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGca                                         <  1:798620/275‑1 (MQ=255)
                                                                                                                                                                                                                                        aCGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATc                                                                                                                                                           >  2:189267/1‑161 (MQ=255)
                                                                                                                                                                                                                                          gTCAGACCCACAAACCGGACAATGCGTCGGGAATACAACCGCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGTCAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACaa                                      <  2:395474/275‑1 (MQ=255)
                                                                                                                                                                                                                                            cAGAACCCCAAACCGGGCAATGCGGCGGGAATAACACCCCACGGGTATCTTCCGGGCGTTACGAAAGCACGACCTTAACCACCTGCGGGATCCCGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAg                                    <  2:481830/275‑1 (MQ=255)
                                                                                                                                                                                                                                            cAGAACCACATACCGGACAATGCGTCGGGAATACACCCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  1:189267/161‑1 (MQ=255)
                                                                                                                                                                                                                                             aGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAgg                                   <  1:211704/275‑1 (MQ=255)
                                                                                                                                                                                                                                                  cccgtacctgcaaaTGCCTCGGGAATACAACCCCACGGGCATCTTCCGGGTGTTCATAAAGCACGACTTTAACCACCGTAGGGATCACGTCGCCAGCGCGGCTAATCACCACTTTCTCGCCAATTCGTAAACCAAGCCGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCCCGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATc                              <  1:436710/263‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccagaccgaaaacggcctCGTGAATACAACCTCACGGGTATCTTCAGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGGTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATc                              <  1:549720/257‑1 (MQ=255)
                                                                                                                                                                                                                                                    caTACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGtt                                                                                                                                                                     >  1:78485/1‑139 (MQ=255)
                                                                                                                                                                                                                                                        ccGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGAt                                                                                                                                                                 <  2:78485/139‑1 (MQ=255)
                                                                                                                                                                                                                                                        ccGAACAATGCTGCGGGAATACAACCTCCCGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCCGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTcc                        <  1:838431/275‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaTGGGTCGGAAATACAACCCCACGGGGATCTTCCGGGCGGTCAGAAAGACCGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTAGCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGcc                  <  2:785800/275‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaTGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGAGTTCATCCACATTGTGTAAGGTTGAGTTACTCACCAGCACGCCTGCAACATGGACAGGTTACAGCCGCGCAACAGGCGTAATATCGCCAGTACCCCCACCCTCAAACACGACGTCCCGCCCAAAGGTCAACTGCTCCTgaccc                  >  2:261675/1‑271 (MQ=255)
                                                                                                                                                                                                                                                                tgggTCGGGAAGCCAACCCACCGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTACCCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCgg                <  1:410177/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                     cGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATc                                                                                                                                                           >  1:358716/1‑132 (MQ=255)
                                                                                                                                                                                                                                                                         aaTACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                                                                                                                                       <  2:358716/132‑1 (MQ=255)
                                                                                                                                                                                                                                                                          agaCAACCTCACGGGTATCTTCCGGACGTTCAGAAAGAACGACGTTAACCACCGGCGCGATCACGTCGCCCGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAGGACGTTCTATTTCATCCTCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa       <  1:452876/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTACAACCTCACGGGTATCTTCCGGGCTTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa       <  1:977660/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTaaa       <  2:203523/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCAACCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCCACAGGCGTAATCGCCCCAGTACGCCCAACCTGAACCTCGACGTCACGCCCAAAGGTCATCTGCCCCTGCGCCGGGACTTTacaca     >  1:107625/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                              aaCCTAACGCGTATCTTCCGTGCGGTCAGAACGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCACGACGTTCGATTTCATCCGCATTGTGTAAGGTTGAGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTa  <  1:1029801/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                
ATTTTGGCCCCATACGCTCCAGTCCGGTCAGTTTGCCTGCGGTGAGTTTGAACAGATCTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGGACAGGTTCCAGACGCGCAACAGGCGTAATCGCGCCAGTACGCCCAACCTGAAACTCGACGTCACGCACAAAGGTCATCTGCTCCTGCGCCGGGAATTTAAACGCTA  >  NC_000913/2528701‑2529243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: