Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,817,989 A→C 8.5% intergenic (‑131/+68) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,817,9890AC 8.5% 155.3 / 3.2 67intergenic (‑131/+68)argQ/argZtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (27/29);  new base C (2/5);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCT  >  NC_000913/2817717‑2818259
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                               
cctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATCTTATTTTCCTCTTGA‑‑TATGGATACCCCTATTCACGCCTTATCATTTAGAAGATCGATCCTGCGTGAGGCTTACTCGCCTTGCTGCTCGCTATTCGGACCCTTGCTCAGGCAACGTCatcatc                                                                                                                                                                                                                                                                              >  2:144242/1‑275 (MQ=14)
        cTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTACATCCATATCAATTAAGAGATGTGGCATCCGGGAGGATTACTCGCCTTGCGGCTTGCCCTTCGGGTCGTGGCCAAACCAACTGTACCATCCCttattc                                                                                                                                                                                                                                                                      >  1:873408/1‑270 (MQ=17)
         tGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTTCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                                                                                                                                                                                                                                                                      >  1:622858/1‑274 (MQ=255)
         tGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTCCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                                                                                                                                                                                                                                                                      >  1:953429/1‑274 (MQ=255)
          gCGGGTAATACATGTATCTTTGCGATACCTGCAGTGCTGAGGGTTACGGGGAATAGGGTGCATGCGGGAGGATTCGAACCGCGGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTTA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                                                                                                                                                                                                                                    <  2:823959/275‑1 (MQ=17)
                   aCCGCTATCGCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGTATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                                                                                                                                                                                                                            <  1:1032641/274‑1 (MQ=255)
                            tatgagttcccctcatagaggcatgTTACGGAGAAGATGGTGCATCCGGGAGGATTCGAACCTCCGGCCGCTCGGTTCGTAGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAc                                                                                                                                                                                                                                                   <  1:873381/251‑1 (MQ=14)
                            ccatagATTCCTTCATTGCTGAAGGTTACGAGGAATGTGGTGCAGCCGGGAGGATTCGAACCTCCGACCGCGCGGTTCGTAGCCGATTACTCTATCCAGCTGGGCTACGGATGCAGCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGGGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                                                                                                                                                                                                                                   <  2:228902/269‑1 (MQ=18)
                                          aTTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACACCTTATTCTCCTGCTGA‑‑TATTGATACCGCTACTAAAGCCATACCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGACCGGTGCTAAAGGAACGTTAGCATCCCTAGTGCCTGCGGTTGATTCCTCTACCTCCTCGATAACtt                                                                                                                                                                                                                                     >  1:564793/1‑274 (MQ=18)
                                                       acgggtaaTATGGGGCATCCGGGAGGATTCGAACCTCCGACCGTTTGGTTCGCGGCCGAGTACTCTATCCAGCCGGGCTACGGATGCATCGGGAATACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGAGCAGCAGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAtt                                                                                                                                                                                                                       <  2:873407/269‑1 (MQ=17)
                                                         ggATAATAGTGTGCATCCGGGAGGATTCGAGCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                                                                                                                                                                     <  1:632839/275‑1 (MQ=255)
                                                          gataaTATGGTGCATCCGGGAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGATTCCTCTATCCAGCTGAGTTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                                                                                    <  1:273388/272‑1 (MQ=255)
                                                             aaTATGGTTCATCCGGGAGGATTCGAACCTCAGACGGCTCGGTTCTTAGACGAGTACGATAGCCAGCTGAGATACGGATGCATTGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGATACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                                                                                  <  1:227789/274‑1 (MQ=18)
                                                                gtGGGGCATCTGGGAAGATTCGACCCTCCGACCGCTCTATTCGTAGCCGATTTCTCTAATCGCCTAAGCTAAGGAAACATCGGGAATACTTAATCGAGTGCTGA‑‑TATAGATACATCTACTAAAGCTATATCTATTAAGAGAGGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                                                                                               <  2:458789/273‑1 (MQ=17)
                                                                      cATCCGGGAGGATTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAGAGGGAAAACTTATTCTACTGCGGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGAGGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                                                                                         <  1:868785/274‑1 (MQ=255)
                                                                       aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACGGCTACTAAAGCCATGTCAATTAAGACATGTTGCATCCGGGAGGATCAATCGCCTTGAGGCTCGCGCTCCGGGCGGTTGCTTAAGAAAACATATCATCCCTAGTGCTTGCGGTTAATTCCGCTACTCCTTAGATACCTTCATTCCTCAAGTTTTCGGAGAATAAgggtc                                                                                                                                                                                                       >  2:511664/1‑272 (MQ=17)
                                                                       aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                                                                                                                                                                                                                           <  1:402773/239‑1 (MQ=255)
                                                                       aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                                                                                                                                                                                                                           >  2:402773/1‑239 (MQ=255)
                                                                       aTCCGGGAGGATTCGAACCCCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTACTTCAACCGCTGA‑‑TATTTATTCCCACCCTAAAGACATAACAATTTAGCAATGGTGCAACCGGGAGGTTTAACCGTCTTTGCGGTCCCCCCTCAGGCCGGGTGTTAAACCAACTTATCCTCCCCTGGTGCCTGTGGTACTGACCGCCCCCTCTTCGATACCTCCAtccttacgcttcccaagaatatggtc                                                                                                                                                                                                         >  2:321930/1‑249 (MQ=2)
                                                                         ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCAt                                                                                                                                                                                                     >  1:598030/1‑275 (MQ=255)
                                                                                  ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATCTTTTTCTCCTCCTTA‑‑TATTAAAACCCCTCTTAAACCATTATCAATTAAGACCTGGTTCTTCTACAAATATTACTCTCCTGACGGCTCGCACATAGGTGAATTGCGAGAGCGCCGTTATCTTCCCTAGTACCATCCGTTACTTCCGCCACATCTTCAATTCCTTCATACCTGCACAATTCAGAAAATATGTTGCAtgccgacata                                                                                                                                                                                            >  2:177684/1‑266 (MQ=9)
                                                                                  ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGAGGCATCGGGAAAACTTATTCTACCGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAATCAACGTTATCATCCCTAGTGTCTGCGGTTAATTCCGCTACCCCTTCGATCCCTTCATTGCTGAAGATTTAGGAGAATATGGTCCATCCgggggg                                                                                                                                                                                             >  2:56787/1‑274 (MQ=18)
                                                                                           ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATCTAGGAGAATATGGTGCATCCGGGAGGATTCGAAcc                                                                                                                                                                                    >  1:795524/1‑274 (MQ=255)
                                                                                                    cGGTTCGTTGCAGAGTACTCTACCCAGTTGAGCTACGGATGCATCGGGAAAACTTCTTCTACTGCTGG‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                          <  2:598030/275‑1 (MQ=255)
                                                                                                     ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACCGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                          <  1:56787/274‑1 (MQ=255)
                                                                                                            aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTCCTGA‑‑TATTAATACCGCTACTAAAGCCATATCAATTAAAAGATGTTGAATCCGGGAGGATTACTCGCCCTGCGGCCCGCCTCTCGGGCCGTTGCTAAAGCCACGTTATCTTCCCAAGTGCCCTCGGTTAATCCCGCTACCCCTTCGATACCTTCCTTGCTGAAGATTTCGGAAAAAAAGATTCAACCGGAAGGCATCCACACTCCGAACGCACGGCTCGt                                                                                                                                                                  >  2:581550/1‑275 (MQ=17)
                                                                                                                gagtatcttttCCAGCTGAGCTACGGATGATACGGGAAAACTTATTCTAATGCGGT‑‑TATTTATACCGCTACTAAAGCCATATTAATTAATGGATGGTGCATCGAGGAGCATTACTCGCACTGCGGCGCTCGTCTGGGGCCGCAGATATAGCTACGATACCGTTCCTGGTGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                                                                                              <  2:622858/265‑1 (MQ=17)
                                                                                                                 aGTACTCTATTCAGCTGAGCTACGGATGCATCTGGAAACCTTATTCTACTGCTTA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                                                                                              <  1:414704/274‑1 (MQ=255)
                                                                                                                 aGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTGGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCCCTTCGATACCTTCATTGCTGAAGATTTCGGCGAATATGGTGCATCCGGGAGGATCCGAACCTCCGACCGACCGCGTCGTAGcc                                                                                                                                                              >  1:591266/1‑274 (MQ=255)
                                                                                                                        tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCCCCTTGCGGCTCGGCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGCGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc                                                                                                                                                      >  1:252263/1‑275 (MQ=255)
                                                                                                                             aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGCTTCGAACCGCCGACCGCCCGGTTCCTAGCCGCGTActc                                                                                                                                                      >  2:535594/1‑270 (MQ=255)
                                                                                                                              gCTGAGCTACGGATGCAGCGGGAAAACTTATTCTACCGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                                                                                                  <  1:535594/273‑1 (MQ=255)
                                                                                                                                                                  tgctgA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAc                                                                                                             <  1:144242/274‑1 (MQ=255)
                                                                                                                                                                     tgA‑‑TATTGATACCGCTACTAAAGGCATATCAATTAAGTGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                         <  1:436987/275‑1 (MQ=255)
                                                                                                                                                                                tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGGGTAATCTATCCAGCTGACCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTgc                                                                                                 >  1:1060189/1‑273 (MQ=255)
                                                                                                                                                                                tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                                                                                 >  1:817164/1‑274 (MQ=255)
                                                                                                                                                                                   cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTTCCAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGCAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTCCTCTATCCAGCTGAGTTACGGACGCATCGGGAAAtttactttacctctgcattttatcc                                                                                              >  2:1031979/1‑259 (MQ=17)
                                                                                                                                                                                   cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGCTATTGATccc                                                                                             >  1:386290/1‑275 (MQ=255)
                                                                                                                                                                                   cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGACTGCGGTTAATTCGGCTACCTCTTCGATACCTTCATTTCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAGCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAcc                                                                                             >  1:935900/1‑275 (MQ=255)
                                                                                                                                                                                   cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGCCTCGCCCTTCTGGCCGTTTCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCTCTACCCCTTCGATACCTTCATTGCTGAAGATTTCGGACAATATGGTGCATGCGGGAGGAATCGAACCTCCGACGGCTCGGATCGTAGCCGAGTACTCCATCCAGCTGACCTACGACTCAATCGGGAAatttatttagcgtcctaaatttatacc                                                                                             >  2:679268/1‑256 (MQ=17)
                                                                                                                                                                                    tctcctaAAGCGATATTAATTAAGAGATGGTGCATCCGTGTGTATTACTCGCCTTGCGGCTCGCCCGTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGGGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                                                                                            <  1:121121/271‑1 (MQ=255)
                                                                                                                                                                                    gtcactaagcgcATGTCATTTAGTAGATGGTGCGGCCGGGAGGAATACTCGCCTTGCGTCTCGCCCCGCGGGCGTTTGCTATAGAAACGTGATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCAGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                                                                                            <  2:953428/264‑1 (MQ=17)
                                                                                                                                                                                    gCTACTGAAGCCCTATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCTGCTCGCCCTTCGGGCCGTTTCTAAAGCAACGTTATCATCCCTAGTTCCTGCGGTTAATTCCGCTGCCTCTTCGATACCTTCATTGGTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                                                                                            <  1:511664/275‑1 (MQ=255)
                                                                                                                                                                                     ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAc                                                                                                                                                                              >  2:494659/1‑192 (MQ=255)
                                                                                                                                                                                     ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTACGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATCCATCGGGAAATTTATTTTACTGCTGATCTTGATCCCga                                                                                           >  1:79150/1‑274 (MQ=255)
                                                                                                                                                                                         taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                          <  1:494659/192‑1 (MQ=255)
                                                                                                                                                                                         gaaagcgatatttgttggggagtGGTGTGTCCGGGGGGGTTGCTCTCCCTGCGGTTCGCCCTTCGGGCCGTTGCTGAAGCAACGTTATCCTCCCTAGTGCCTGCGGTTAATGCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctact                                                                                       <  2:736377/253‑1 (MQ=14)
                                                                                                                                                                                                  atCAATTAAGGGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata                                                                              <  1:375389/275‑1 (MQ=255)
                                                                                                                                                                                                  aagatgcaggagagtgggCATACGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata                                                                              <  1:505795/257‑1 (MQ=11)
                                                                                                                                                                                                   tCAATTAAGATATGGTGAATCCGGGAGGATTACTCGCCTCGCGTCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCatat                                                                             <  1:177684/275‑1 (MQ=255)
                                                                                                                                                                                                   agatgtttataagagacggtCCGGGAGGATTATTCGCCTTGCGGCTGGCCCTTCGGGCCGTGGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata                                                                              <  2:358693/255‑1 (MQ=17)
                                                                                                                                                                                                    cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATc                                                                            <  2:817163/275‑1 (MQ=255)
                                                                                                                                                                                                        taagagagatggCATCCGGGGGAAGTAGTCTCATTGCGGCTCGCCCTTCGGGCCGTTGCTAGAGCAACGTTATCCTCCCTAGTGCCTGCGGTTAATGCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGGTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGt                                                                        <  1:181316/264‑1 (MQ=14)
                                                                                                                                                                                                               tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCCCGGTTCGTAGCCGCGTCCTCTATCCAGCTGATCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACAGCTACTAAAGCGCTATCAAGTAAgagag                                                                 >  2:928758/1‑274 (MQ=255)
                                                                                                                                                                                                                ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                                                                                               >  2:1012374/1‑228 (MQ=255)
                                                                                                                                                                                                                 gTGCATCCGGGAGGATTACTCGCCTGGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAACATCCCTAGTCCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATTCGGGCGGATTCGAACCTCCGCCCTCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTCTTTTACTGCCGATATTGATACCCACACCAACTCCCTACAAAGTATGAGATgg                                                               >  2:529524/1‑275 (MQ=17)
                                                                                                                                                                                                                   ttaacgggtcgcgttgaccGCCTGGCGGTTCGCCGTTCGGGCGTTTGCTAACGCAACGTTAGCATCCTTGGTGCCTGCGGTTAATCCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTCCGAAGAATATGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGt                                                              <  2:523818/256‑1 (MQ=14)
                                                                                                                                                                                                                   gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTg                                                                                                                                                                                                                                >  1:155317/1‑112 (MQ=255)
                                                                                                                                                                                                                    cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                           <  1:1012375/228‑1 (MQ=255)
                                                                                                                                                                                                                      tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAACTTTATTTTACTTCTGATATTGATCCCGCTACTAAAGc                                                                                  >  2:505795/1‑250 (MQ=11)
                                                                                                                                                                                                                       ccGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGa                                                                                                                                                                                                                            <  2:155317/112‑1 (MQ=255)
                                                                                                                                                                                                                                 tACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACATTTATTTTACTGCTGATATTGATCCCGCTACTAAAGc                                                                                  >  1:358693/1‑240 (MQ=255)
                                                                                                                                                                                                                                   cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCCTCGGGAAATTTATTTTACTGCTGATATTTATACCGCTACTAAAGCCATATCCAGTAAGCAATTGTGCATCCggtcggaatc                                              >  2:1064996/1‑266 (MQ=255)
                                                                                                                                                                                                                                        ccctGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGTGACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAcc                                         <  1:566860/272‑1 (MQ=255)
                                                                                                                                                                                                                                                            gggcattGCTAAAGCAACGTTATCTTCCCTACTGCCTGCTGTTAATTCAGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATAGGGTGCATCCGGGCGGATTCGAACCTCCGACCGCTCGGTGCGTATCCGAGTACTCTAACCAGCTGAGCTACGGATGCATCGGGAAATTTATATTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                      <  2:386290/270‑1 (MQ=18)
                                                                                                                                                                                                                                                                 tggCTAAATCAACGTTATCAGCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                 <  1:581550/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTATGAGATGGTTCATCTGGGAGGATTCGAACCTCCGACCTCTCGGTTCGTAGct                   >  1:1047027/1‑271 (MQ=255)
                                                                                                                                                                                                                                                                      aaaGAAACGTTATCCTCCCGAGTGCTTGCGGTTAATTCCGCTACCTCTTCGATACCTGCATTGCTGAAGATTTCGGAGAATATGGTGCTCCCGGGAGGATTCTAACCTCCGACCGCCCGGTTCGTAGCCGAGTACGCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCGGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAct            <  1:1069372/275‑1 (MQ=25)
                                                                                                                                                                                                                                                                                gatcatcCCTTGGGCGTGTGATTAATTTCGTTGCCACTTTGATGCCTTCCGTGATAAAGATGTCGGGGGATATGGTGCATCCGGCAGGATGCGATCCTCTGCTCGGGCTGTGCGTAGCCGAAGATTTGATCCAGCTCGGCCTCGGATGCATTGTGGAAGGTGTATTACTGCCGATATTGATACCGTTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt  <  2:79150/274‑1 (MQ=16)
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                               
CCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCT  >  NC_000913/2817717‑2818259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: