Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,818,011 T→A 6.7% intergenic (‑153/+46) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,0110TA 6.7% 195.8 / 2.6 70intergenic (‑153/+46)argQ/argZtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base A (2/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAA  >  NC_000913/2817745‑2818280
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                             
tatgagttcccctcatagaggcatgTTACGGAGAAGATGGTGCATCCGGGAGGATTCGAACCTCCGGCCGCTCGGTTCGTAGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAc                                                                                                                                                                                                                                                                        <  1:873381/251‑1 (MQ=14)
ccatagATTCCTTCATTGCTGAAGGTTACGAGGAATGTGGTGCAGCCGGGAGGATTCGAACCTCCGACCGCGCGGTTCGTAGCCGATTACTCTATCCAGCTGGGCTACGGATGCAGCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGGGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                                                                                                                                                                                                                                                        <  2:228902/269‑1 (MQ=18)
              aTTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACACCTTATTCTCCTGCTGA‑‑TATTGATACCGCTACTAAAGCCATACCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGACCGGTGCTAAAGGAACGTTAGCATCCCTAGTGCCTGCGGTTGATTCCTCTACCTCCTCGATAACtt                                                                                                                                                                                                                                                          >  1:564793/1‑274 (MQ=18)
                           acgggtaaTATGGGGCATCCGGGAGGATTCGAACCTCCGACCGTTTGGTTCGCGGCCGAGTACTCTATCCAGCCGGGCTACGGATGCATCGGGAATACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGAGCAGCAGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAtt                                                                                                                                                                                                                                            <  2:873407/269‑1 (MQ=17)
                             ggATAATAGTGTGCATCCGGGAGGATTCGAGCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                                                                                                                                                                                          <  1:632839/275‑1 (MQ=255)
                              gataaTATGGTGCATCCGGGAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGATTCCTCTATCCAGCTGAGTTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                                                                                                         <  1:273388/272‑1 (MQ=255)
                                 aaTATGGTTCATCCGGGAGGATTCGAACCTCAGACGGCTCGGTTCTTAGACGAGTACGATAGCCAGCTGAGATACGGATGCATTGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGATACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                                                                                                       <  1:227789/274‑1 (MQ=18)
                                    gtGGGGCATCTGGGAAGATTCGACCCTCCGACCGCTCTATTCGTAGCCGATTTCTCTAATCGCCTAAGCTAAGGAAACATCGGGAATACTTAATCGAGTGCTGA‑‑TATAGATACATCTACTAAAGCTATATCTATTAAGAGAGGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                                                                                                                    <  2:458789/273‑1 (MQ=17)
                                          cATCCGGGAGGATTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAGAGGGAAAACTTATTCTACTGCGGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGAGGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                                                                                                              <  1:868785/274‑1 (MQ=255)
                                           aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACGGCTACTAAAGCCATGTCAATTAAGACATGTTGCATCCGGGAGGATCAATCGCCTTGAGGCTCGCGCTCCGGGCGGTTGCTTAAGAAAACATATCATCCCTAGTGCTTGCGGTTAATTCCGCTACTCCTTAGATACCTTCATTCCTCAAGTTTTCGGAGAATAAgggtc                                                                                                                                                                                                                            >  2:511664/1‑272 (MQ=17)
                                           aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                                                                                                                                                                                                                                                <  1:402773/239‑1 (MQ=255)
                                           aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                                                                                                                                                                                                                                                >  2:402773/1‑239 (MQ=255)
                                           aTCCGGGAGGATTCGAACCCCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTACTTCAACCGCTGA‑‑TATTTATTCCCACCCTAAAGACATAACAATTTAGCAATGGTGCAACCGGGAGGTTTAACCGTCTTTGCGGTCCCCCCTCAGGCCGGGTGTTAAACCAACTTATCCTCCCCTGGTGCCTGTGGTACTGACCGCCCCCTCTTCGATACCTCCAtccttacgcttcccaagaatatggtc                                                                                                                                                                                                                              >  2:321930/1‑249 (MQ=2)
                                             ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCAt                                                                                                                                                                                                                          >  1:598030/1‑275 (MQ=255)
                                                      ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATCTTTTTCTCCTCCTTA‑‑TATTAAAACCCCTCTTAAACCATTATCAATTAAGACCTGGTTCTTCTACAAATATTACTCTCCTGACGGCTCGCACATAGGTGAATTGCGAGAGCGCCGTTATCTTCCCTAGTACCATCCGTTACTTCCGCCACATCTTCAATTCCTTCATACCTGCACAATTCAGAAAATATGTTGCAtgccgacata                                                                                                                                                                                                                 >  2:177684/1‑266 (MQ=9)
                                                      ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGAGGCATCGGGAAAACTTATTCTACCGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAATCAACGTTATCATCCCTAGTGTCTGCGGTTAATTCCGCTACCCCTTCGATCCCTTCATTGCTGAAGATTTAGGAGAATATGGTCCATCCgggggg                                                                                                                                                                                                                  >  2:56787/1‑274 (MQ=18)
                                                               ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATCTAGGAGAATATGGTGCATCCGGGAGGATTCGAAcc                                                                                                                                                                                                         >  1:795524/1‑274 (MQ=255)
                                                                        cGGTTCGTTGCAGAGTACTCTACCCAGTTGAGCTACGGATGCATCGGGAAAACTTCTTCTACTGCTGG‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                               <  2:598030/275‑1 (MQ=255)
                                                                         ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACCGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                               <  1:56787/274‑1 (MQ=255)
                                                                                aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTCCTGA‑‑TATTAATACCGCTACTAAAGCCATATCAATTAAAAGATGTTGAATCCGGGAGGATTACTCGCCCTGCGGCCCGCCTCTCGGGCCGTTGCTAAAGCCACGTTATCTTCCCAAGTGCCCTCGGTTAATCCCGCTACCCCTTCGATACCTTCCTTGCTGAAGATTTCGGAAAAAAAGATTCAACCGGAAGGCATCCACACTCCGAACGCACGGCTCGt                                                                                                                                                                                       >  2:581550/1‑275 (MQ=17)
                                                                                    gagtatcttttCCAGCTGAGCTACGGATGATACGGGAAAACTTATTCTAATGCGGT‑‑TATTTATACCGCTACTAAAGCCATATTAATTAATGGATGGTGCATCGAGGAGCATTACTCGCACTGCGGCGCTCGTCTGGGGCCGCAGATATAGCTACGATACCGTTCCTGGTGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                                                                                                                   <  2:622858/265‑1 (MQ=17)
                                                                                     aGTACTCTATTCAGCTGAGCTACGGATGCATCTGGAAACCTTATTCTACTGCTTA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                                                                                                                   <  1:414704/274‑1 (MQ=255)
                                                                                     aGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTGGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCCCTTCGATACCTTCATTGCTGAAGATTTCGGCGAATATGGTGCATCCGGGAGGATCCGAACCTCCGACCGACCGCGTCGTAGcc                                                                                                                                                                                   >  1:591266/1‑274 (MQ=255)
                                                                                            tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCCCCTTGCGGCTCGGCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGCGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc                                                                                                                                                                           >  1:252263/1‑275 (MQ=255)
                                                                                                 aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGCTTCGAACCGCCGACCGCCCGGTTCCTAGCCGCGTActc                                                                                                                                                                           >  2:535594/1‑270 (MQ=255)
                                                                                                  gCTGAGCTACGGATGCAGCGGGAAAACTTATTCTACCGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                                                                                                                       <  1:535594/273‑1 (MQ=255)
                                                                                                                                      tgctgA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAc                                                                                                                                  <  1:144242/274‑1 (MQ=255)
                                                                                                                                         tgA‑‑TATTGATACCGCTACTAAAGGCATATCAATTAAGTGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                                                                                                              <  1:436987/275‑1 (MQ=255)
                                                                                                                                                    tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGGGTAATCTATCCAGCTGACCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTgc                                                                                                                      >  1:1060189/1‑273 (MQ=255)
                                                                                                                                                    tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                                                                                                      >  1:817164/1‑274 (MQ=255)
                                                                                                                                                       cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTTCCAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGCAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTCCTCTATCCAGCTGAGTTACGGACGCATCGGGAAAtttactttacctctgcattttatcc                                                                                                                   >  2:1031979/1‑259 (MQ=17)
                                                                                                                                                       cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGCTATTGATccc                                                                                                                  >  1:386290/1‑275 (MQ=255)
                                                                                                                                                       cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGACTGCGGTTAATTCGGCTACCTCTTCGATACCTTCATTTCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAGCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAcc                                                                                                                  >  1:935900/1‑275 (MQ=255)
                                                                                                                                                       cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGCCTCGCCCTTCTGGCCGTTTCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCTCTACCCCTTCGATACCTTCATTGCTGAAGATTTCGGACAATATGGTGCATGCGGGAGGAATCGAACCTCCGACGGCTCGGATCGTAGCCGAGTACTCCATCCAGCTGACCTACGACTCAATCGGGAAatttatttagcgtcctaaatttatacc                                                                                                                  >  2:679268/1‑256 (MQ=17)
                                                                                                                                                        tctcctaAAGCGATATTAATTAAGAGATGGTGCATCCGTGTGTATTACTCGCCTTGCGGCTCGCCCGTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGGGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                                                                                                                 <  1:121121/271‑1 (MQ=255)
                                                                                                                                                        gtcactaagcgcATGTCATTTAGTAGATGGTGCGGCCGGGAGGAATACTCGCCTTGCGTCTCGCCCCGCGGGCGTTTGCTATAGAAACGTGATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCAGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                                                                                                                 <  2:953428/264‑1 (MQ=17)
                                                                                                                                                        gCTACTGAAGCCCTATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCTGCTCGCCCTTCGGGCCGTTTCTAAAGCAACGTTATCATCCCTAGTTCCTGCGGTTAATTCCGCTGCCTCTTCGATACCTTCATTGGTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                                                                                                                 <  1:511664/275‑1 (MQ=255)
                                                                                                                                                         ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAc                                                                                                                                                                                                   >  2:494659/1‑192 (MQ=255)
                                                                                                                                                         ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTACGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATCCATCGGGAAATTTATTTTACTGCTGATCTTGATCCCga                                                                                                                >  1:79150/1‑274 (MQ=255)
                                                                                                                                                             taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                               <  1:494659/192‑1 (MQ=255)
                                                                                                                                                             gaaagcgatatttgttggggagtGGTGTGTCCGGGGGGGTTGCTCTCCCTGCGGTTCGCCCTTCGGGCCGTTGCTGAAGCAACGTTATCCTCCCTAGTGCCTGCGGTTAATGCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctact                                                                                                            <  2:736377/253‑1 (MQ=14)
                                                                                                                                                                      atCAATTAAGGGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata                                                                                                   <  1:375389/275‑1 (MQ=255)
                                                                                                                                                                      aagatgcaggagagtgggCATACGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata                                                                                                   <  1:505795/257‑1 (MQ=11)
                                                                                                                                                                       tCAATTAAGATATGGTGAATCCGGGAGGATTACTCGCCTCGCGTCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCatat                                                                                                  <  1:177684/275‑1 (MQ=255)
                                                                                                                                                                       agatgtttataagagacggtCCGGGAGGATTATTCGCCTTGCGGCTGGCCCTTCGGGCCGTGGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata                                                                                                   <  2:358693/255‑1 (MQ=17)
                                                                                                                                                                        cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATc                                                                                                 <  2:817163/275‑1 (MQ=255)
                                                                                                                                                                            taagagagatggCATCCGGGGGAAGTAGTCTCATTGCGGCTCGCCCTTCGGGCCGTTGCTAGAGCAACGTTATCCTCCCTAGTGCCTGCGGTTAATGCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGGTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGt                                                                                             <  1:181316/264‑1 (MQ=14)
                                                                                                                                                                                   tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCCCGGTTCGTAGCCGCGTCCTCTATCCAGCTGATCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACAGCTACTAAAGCGCTATCAAGTAAgagag                                                                                      >  2:928758/1‑274 (MQ=255)
                                                                                                                                                                                    ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAtttt                                                                                                                                    >  2:1012374/1‑228 (MQ=255)
                                                                                                                                                                                     gTGCATCCGGGAGGATTACTCGCCTGGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAACATCCCTAGTCCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATTCGGGCGGATTCGAACCTCCGCCCTCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTCTTTTACTGCCGATATTGATACCCACACCAACTCCCTACAAAGTATGAGATgg                                                                                    >  2:529524/1‑275 (MQ=17)
                                                                                                                                                                                       ttaacgggtcgcgttgaccGCCTGGCGGTTCGCCGTTCGGGCGTTTGCTAACGCAACGTTAGCATCCTTGGTGCCTGCGGTTAATCCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTCCGAAGAATATGGTGCATCCGGGAGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGt                                                                                   <  2:523818/256‑1 (MQ=14)
                                                                                                                                                                                       gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTg                                                                                                                                                                                                                                                     >  1:155317/1‑112 (MQ=255)
                                                                                                                                                                                        cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                                                                                                                <  1:1012375/228‑1 (MQ=255)
                                                                                                                                                                                          tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAACTTTATTTTACTTCTGATATTGATCCCGCTACTAAAGc                                                                                                       >  2:505795/1‑250 (MQ=11)
                                                                                                                                                                                           ccGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGa                                                                                                                                                                                                                                                 <  2:155317/112‑1 (MQ=255)
                                                                                                                                                                                                     tACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACATTTATTTTACTGCTGATATTGATCCCGCTACTAAAGc                                                                                                       >  1:358693/1‑240 (MQ=255)
                                                                                                                                                                                                       cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCCTCGGGAAATTTATTTTACTGCTGATATTTATACCGCTACTAAAGCCATATCCAGTAAGCAATTGTGCATCCggtcggaatc                                                                   >  2:1064996/1‑266 (MQ=255)
                                                                                                                                                                                                            ccctGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGTGACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAcc                                                              <  1:566860/272‑1 (MQ=255)
                                                                                                                                                                                                                                gggcattGCTAAAGCAACGTTATCTTCCCTACTGCCTGCTGTTAATTCAGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATAGGGTGCATCCGGGCGGATTCGAACCTCCGACCGCTCGGTGCGTATCCGAGTACTCTAACCAGCTGAGCTACGGATGCATCGGGAAATTTATATTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                           <  2:386290/270‑1 (MQ=18)
                                                                                                                                                                                                                                     tggCTAAATCAACGTTATCAGCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                      <  1:581550/273‑1 (MQ=255)
                                                                                                                                                                                                                                      tGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTATGAGATGGTTCATCTGGGAGGATTCGAACCTCCGACCTCTCGGTTCGTAGct                                        >  1:1047027/1‑271 (MQ=255)
                                                                                                                                                                                                                                          aaaGAAACGTTATCCTCCCGAGTGCTTGCGGTTAATTCCGCTACCTCTTCGATACCTGCATTGCTGAAGATTTCGGAGAATATGGTGCTCCCGGGAGGATTCTAACCTCCGACCGCCCGGTTCGTAGCCGAGTACGCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCGGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAct                                 <  1:1069372/275‑1 (MQ=25)
                                                                                                                                                                                                                                                    gatcatcCCTTGGGCGTGTGATTAATTTCGTTGCCACTTTGATGCCTTCCGTGATAAAGATGTCGGGGGATATGGTGCATCCGGCAGGATGCGATCCTCTGCTCGGGCTGTGCGTAGCCGAAGATTTGATCCAGCTCGGCCTCGGATGCATTGTGGAAGGTGTATTACTGCCGATATTGATACCGTTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                       <  2:79150/274‑1 (MQ=16)
                                                                                                                                                                                                                                                               aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTACGAGATGGTGCATCCGGGAGGATTCGAACCCCCGACCGCCCGGTTCGTAGCCgcagctcctaccctgttgagctccggt             >  1:1062648/1‑249 (MQ=17)
                                                                                                                                                                                                                                                               aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCAGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa             >  1:256218/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCGACTAAAGCCATATAAAGTAAGAGATGGTGCTTCCGGGAGGATTCGAACCCCCGACGGCTCGGTTCGGAGCCGCAGACTCTATCCAACTGACCTCCGGAGGCAt        >  1:613651/1‑274 (MQ=17)
                                                                                                                                                                                                                                                                     tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGCAATTTATTTTACTGCTGATATTGATACCGATCCTAAAACCATATCAAGTAAGAGATGGTGCATGCGTGCGGATTCGAAGCTCCGACCGCTCGGGTCTTAGCCGAGTCCTTTGTACAGCTGAGCTACggctccacc       >  1:229745/1‑268 (MQ=17)
                                                                                                                                                                                                                                                                     tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTACCCAGCTGAGCTACGGATTCATc       >  1:388021/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                     tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACCGATGCATCGGGAAATGTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTACGAGATGGTGCACCCGGGAGGATTCGAACCTCCGACCGGTTGGTACGTAGCCGAGTACTTTTTCCTGCTGAGCTACGTATGCacc       >  1:59420/1‑273 (MQ=17)
                                                                                                                                                                                                                                                                          ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATGTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaa  <  1:1031980/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                             
TCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAA  >  NC_000913/2817745‑2818280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: