Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,296,189	A→G	20.1%	intergenic (+396/+248)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,189	0	A	G	20.1%	38.4 / 4.9	29	intergenic (+396/+248)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base A (15/8);  new base G (4/2);  total (19/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTC  >  NC_000913/4295917‑4296457                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                              tacggCGTTTATTCCGGGCCCGAAGACACCGCGTAATGCGACGCTTAACGGTTCTTATCAGGCCTACGCCAGACAGCGGAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                                              <  1:1014219/271‑1 (MQ=11)               cgcaacggcgcgtacggccgttggcggcgCTTAACGGTTTGTATCAGGCCTAGGCCAGACAGCGCAATAGCCTGATTTAGTGTGATTTTGTGGGGCGTGTAAGGCGTTTACGCCGCGTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                                               <  2:154488/252‑1 (MQ=11)                gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACTGCGTTTTTGCCGCATCCGACCTCACCGCCTGATGCGACGCTTAACGCGCTTTATCCGGc                                                                                                                                                                                                                                                               >  2:1064468/1‑274 (MQ=255)                 cagccGTCATCAACGCCTGATGCCACGCTTACCGCGTCTTATCAGTCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                                               <  1:628048/270‑1 (MQ=16)                       acggcAAAGCCTGATGCGACGTGTAACGTGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCa                                                                                                                                                                                                                                                       <  1:716802/271‑1 (MQ=255)                            aagcccctaTGCGGCGGTTACCGGGTTTTAGGGGGCATAGGCCAGACACCGCAATAGCAGTATTTAGGTTGATTTTGTAGGTCGGATAAGGCGTTTCCGCCGGATCCGACATAAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACa                                                                                                                                                                                                                                                   <  2:878334/266‑1 (MQ=11)                              cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCCTCTTATCAGGCCTTCGCCAGACGgcg                                                                                                                                                                                                                                                >  1:238381/1‑275 (MQ=255)                                      gCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                                                                                                                                                                         <  1:1050270/274‑1 (MQ=18)                                                     tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACCCTTAACTCGTCTTATCAGGCCTCTCCCACCCCGCGAAATAGCCTGATTTACCCTGact                                                                                                                                                                                                                         >  2:792481/1‑273 (MQ=11)                                                      cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGCTTTAGCGTTATTTTGGAGGTCGGATACGGCGTTTATGCCGCATCAGACATAAACGCCGGATGCGACGATTGACGCGTCTTATCAGGCCTGCGCCAGCCGGCGCATAAGACTGAATTAGCGCGAttt                                                                                                                                                                                                                        >  2:55822/1‑275 (MQ=11)                                                           cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                                                                                                                                                                                                                   >  1:821159/1‑275 (MQ=33)                                                              gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                                                                                                                                                                 >  1:211187/1‑274 (MQ=18)                                                                   cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTGATTTCGTAGGTCGGATAACGCGTTTATGCCGCATCCCAAATAAACGCCTGCTGCGACGCTTACCGCGTCTGTACAGGCCTCTCCCCAACTGCGCAATAGCCTGATTTacccttatacttaagttccgat                                                                                                                                                                                                            >  2:388474/1‑253 (MQ=11)                                                                   cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCCTCTTATCAGGCCTACGCCAGACCGAGGAACAGCCTTATTTCGCGTTATTCTGTAGCTCGGAt                                                                                                                                                                                                            >  2:759885/1‑274 (MQ=11)                                                                   cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACTCCAGACAGCGCATTAGCCTTATTTAGCGTGATTTTGTAGGCCGGAt                                                                                                                                                                                                            >  1:629666/1‑274 (MQ=18)                                                                   cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGTCAGACAGCGCCAAATCCTGCTTTAGCGTGATTTTGTAGGTCGGaca                                                                                                                                                                                                           >  2:733463/1‑273 (MQ=16)                                                                        gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACCTCCACGCCTGCTGCGACGCTTACCGCGTCTTCTCAGGCCTACCCCAGCAAGCGCACTAGCCTGCTTTAGCGTGATTTTGTAGGCCGGATAAGGc                                                                                                                                                                                                       >  2:778543/1‑274 (MQ=11)                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCATTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGGGTCTTATCCGGCTTACGCCACACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGCCGGATCAGGCGTTTac                                                                                                                                                                                                 >  2:133479/1‑274 (MQ=11)                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                                                                                                                                                                 >  1:418734/1‑227 (MQ=34)                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACCACTGATGCGTCGCTTAACGCGTCTTATCAGCCCTACGCCACAAACGGGAATAGCCTTATTTAGCGTGATTTTGGAGTTCGGATACGGGCTTTa                                                                                                                                                                                                  >  2:1058365/1‑274 (MQ=11)                                                                              gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGTGCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTacg                                                                                                                                                                                                <  1:146558/275‑3 (MQ=12)                                                                                 aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                                             <  2:418734/227‑1 (MQ=34)                                                                                                                           tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTCGCGTGATTTTGTAGGTCGGATCAGGCGTTTATGCCGCATCCGCCATCAACCCCTGATGCAACGATTAACGCGTCTTATCATGCCTTCGCCAGACCCCCCAATAGCCGTACGTAGCGCGATGTTGTAGAGCGGCTCGTGCGTTTACGCCGCATCCGACCACAACCACAGATGCCACACTTGActgtgctca                                                                                                                                                    >  2:844104/1‑266 (MQ=11)                                                                                                                                                                                                                                       ggctttacgCCGCATCCGTCATCCACGCCTGCTGCGACGCTTGCCGCGTCTTAGCAGGCCGACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgt                                       >  2:798814‑M2/4‑249 (MQ=255)                                                                                                                                                                                                                                       gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCATGATGCGACGCTTGCCGCGTCTTATCCGGCCTACGCTAGACAGCGCAATAGCCTGCTTTGGCGTGATTTTCTCGTTCGGATAGGCAGTTCATGCCGCCT‑CGGCACTAATGACCTGATGCgctccttaactcc                                       >  2:1024572/1‑262 (MQ=11)                                                                                                                                                                                                                                             acgccGCATCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttat                                  >  1:800610‑M2/3‑243 (MQ=255)                                                                                                                                                                                                                                                   cATCCGACACAAACGCCGGAGGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCAGAATCCGACATAAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTTTTTTGATTTCGTCTGATTTTGTAGGTCGGATCAGGCGTTTACGCCGCATCCTCCCTTTCTC‑CCTTTTGCGATTCTTGCCGCGTCTTATCAGGCCt                          <  1:279069/275‑1 (MQ=11)                                                                                                                                                                                                                                                             aaagtccTGATGCGAGGCTTGCCGCGGCTTATCAGGCTTACGCCAGCAAGCGAAAGATCCTGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGAACCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                <  2:143056/270‑1 (MQ=11)                                                                                                                                                                                                                                                                cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt              >  1:573335/1‑274 (MQ=18)                                                                                                                                                                                                                                                                            cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCTTTATTTAGCGTGATTTTTTAGGTCGCATAAGGCGTTTCCGCCGCATCCGACATCaacgcctgatgctacgcttgccgtgtcttctcaggccttcccccgccgtctaaataacctgat  <  2:146447‑M2/275‑64 (MQ=255)                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                              TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTC  >  NC_000913/4295917‑4296457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑