Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,245,959 G→A 3.7% A473V (GCG→GTG)  treA ← periplasmic trehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,245,9590GA 3.7% 345.7 / 2.7 112A473V (GCG→GTG) treAperiplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base G (54/53);  new base A (2/2);  total (57/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 115 total aligned reads displayed.

TGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCTTGTTGAT  >  NC_000913/1245685‑1246232
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                 
ggggggTGGTTCCTCTTTGGGTGAGGGTTGCGTCGTTGCTGGCTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGGCTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCg                                                                                                                                                                                                                                                                                   <  2:959488/272‑1 (MQ=255)
  tgGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCg                                                                                                                                                                                                                                                                                   <  1:267557/273‑1 (MQ=255)
   gggTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGCTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCAACCAGCTTTTCCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCa                                                                                                                                                                                                                                                                                >  2:54458/1‑275 (MQ=255)
   gggTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTCTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCACTTTCAGCGTCACGCCATTGGTCCAGCCACAGCCATCCTGTAATGGATATTCGCCACCGCCTCCCCCCGGCCCGCTGGTGCTGCCATCATATCTTTCCTCCCGCTCTCTCTCCCGGTCATCGTTGTGCTCCACCTTGGTCAGTAAGTGCCCGCTCATGTCCACCGcc                                                                                                                                                                                                                                                                                 >  2:775023/1‑274 (MQ=255)
   gggTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGACGGACGCGTCCCCGGAACATTGTCACCCGGTTGCTCTTTCGGCCAGATCAAATCCAGCATGTTCAGCATCCCGTAATCGGTCTAGCCAAAGCCCTCCTGTAATGGATATTCAGCACCGCCGCCCCCCGTTCCGGTGGTGCTCACCACATATTTTTCCTCTAGCTTTATCTCCCGCTCACAGGTGTGCTGACCATTTGTCCGGAAGTGCCAAACAATCTCCACCGcc                                                                                                                                                                                                                                                                                 >  2:936326/1‑274 (MQ=255)
         tgCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGCCTTAACGGTCGGACGCCTCGCCGGAACATTGTCACACGGTTGCTCTTTCGCGCAGATCAGATCCACCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTACTGCATATTCGCCCCCGCCGCCCCCCGCTCCGCTGGTGCTGACACCACATTTTTCCCCCAGCCTTTTCTCCCTGTCATCGGTGTGCTGACCACTGGTTAGGCAGGCCCAGTTAATGCCCCCCGACCCCTCtt                                                                                                                                                                                                                                                                          >  2:128992/1‑275 (MQ=255)
         tgCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGATGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCtt                                                                                                                                                                                                                                                                          >  1:567140/1‑275 (MQ=255)
                  ggTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCg                                                                                                                                                                                                                                                                  <  1:288538/274‑1 (MQ=255)
                   gTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTACTGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTa                                                                                                                                                                                                                                                                >  1:1022083/1‑275 (MQ=255)
                    ttGAGGGTTGCGTCGTTTCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTa                                                                                                                                                                                                                                                                <  1:300007/274‑1 (MQ=255)
                    ttGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTa                                                                                                                                                                                                                                                                <  1:660130/274‑1 (MQ=255)
                         ggTTGCGTCGTTGCTGACTTAACGGTCGGCACCGTCGCCGGTACATTGTCATCCGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCCTTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCCCCGCCCCCCCCCGTTCCGGCGGTACTCATATCATATTTTTCCACCACCTCTTTCTCCCGGTCTTAGTGCTGCTGAACATTGTATCGGCAGTGCCCGATCACTTCCATCGCCACCGCTTTTTGCACGCTGtttt                                                                                                                                                                                                                                                           >  2:743111/1‑274 (MQ=255)
                         ggTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGtttt                                                                                                                                                                                                                                                           >  1:138392/1‑274 (MQ=255)
                          gTTGCGTCGTTGCTGACGTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGt                                                                                                                                                                                                                                                         <  1:597032/275‑1 (MQ=255)
                             gCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCCCCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGCAAGGAAGTGCCCGTTCATGTCCATCCCCCCCTCTTTTTGCCCGTAGTTTTGTaa                                                                                                                                                                                                                                                       >  2:564007/1‑274 (MQ=255)
                              cgtcgtTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCCCCTCTTTTTGTCCGTAGTTTTGTact                                                                                                                                                                                                                                                      >  2:474666/1‑272 (MQ=255)
                                  tttGCTTACTTAACGGTCGGAAGCGTCGCCGGAACATTTTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTGTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCtt                                                                                                                                                                                                                                                  <  2:1059177/273‑1 (MQ=255)
                                       tGACTTAACGTTCGGCTGGGTCGCCGGAACATTGTCACACGGTTGCGCTTTCGGGCAGATCAAATCCAGCAGTTTCATCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGTTATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTcg                                                                                                                                                                                                                                            <  2:142625/275‑1 (MQ=255)
                                        gACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCCAATCCAGCATTTTCAGCGTCCCGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCAGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTTCGCTGAACATTGGTCAGGAAGTGCTAGCTAATGTACATCGCCACCTCTCTTTGCCCGTTGTTTTTTAATCCtttctctgc                                                                                                                                                                                                                                           >  2:381611/1‑268 (MQ=255)
                                          cTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTTCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTcgcg                                                                                                                                                                                                                                          <  1:545674/274‑1 (MQ=255)
                                             aagggTCGGACGCGTCGCCGGAACATTTTCACACGGTTGCTCTTTCGGGCATATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGAcc                                                                                                                                                                                                                                       <  1:532092/271‑1 (MQ=255)
                                             aaCGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACCTTGGTCCGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTCTTGCCCGTAGTTTTGTCACCCTTCTTTCCCGAcc                                                                                                                                                                                                                                       >  2:194624/1‑274 (MQ=255)
                                             aaCGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGAcc                                                                                                                                                                                                                                       <  1:907329/274‑1 (MQ=255)
                                             aaCGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGAcc                                                                                                                                                                                                                                       <  2:398218/274‑1 (MQ=255)
                                             aaCGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGCGCAGATCAAATCCAGCATGTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTGCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGAcc                                                                                                                                                                                                                                       <  2:621694/274‑1 (MQ=255)
                                                 gTCGGATGCGTTGCCGGAACATTGTCACACGGTTGCCCTTTCAGGCAGGACAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGGCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTGTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACt                                                                                                                                                                                                                                   <  1:239492/274‑1 (MQ=255)
                                                   cGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACTGTGGTCAGGAAGTGCCAGCTAATGTCCATCGCACCCTCTTTTTGCCCGTAGTTTTTGAATCCTTCTTTCGCGACCCACTGTa                                                                                                                                                                                                                                >  1:519810/1‑275 (MQ=255)
                                                   cGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTa                                                                                                                                                                                                                                >  2:700785/1‑275 (MQ=255)
                                                    ggAAGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGTGCAGATCAAATCCAGCATTTTCAGCGCCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGTTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTaa                                                                                                                                                                                                                               <  1:828435/275‑1 (MQ=255)
                                                      aCGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACg                                                                                                                                                                                                                             <  1:123267/275‑1 (MQ=255)
                                                                      ttGTCACACGGTTGCTCTTTCGGGCAGATCAAACCCAGCATTTTCAGCGTCACGCCATTCGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGCTCCGGCGGTGCTGACATCATATTTTTCCACCAGCTTTTTCCCACGGTCATAGGTGTGCTGACCATTAGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTCTGCCCGCAGTGTATTAATCCTTCTGTCGC‑‑CCCCCTGTACCGGTCCCCCGCCATTTgg                                                                                                                                                                                                             >  2:932052/1‑273 (MQ=255)
                                                                       tGTCACGCGGTTGCTCTCTCGGTCAAATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGgc                                                                                                                                                                                                            <  1:606357/275‑1 (MQ=255)
                                                                        ttcccccGGTTGCGCTTTCGGGAAGATCAAATCCAGCATTTTCCGCGTCACCCCCTTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGgcg                                                                                                                                                                                                           <  1:401605/269‑1 (MQ=255)
                                                                        gTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGgc                                                                                                                                                                                                            <  1:169278/274‑1 (MQ=255)
                                                                                   gCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCGTATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCCTTTGGCGCATCCCAttg                                                                                                                                                                                                 >  1:12695/1‑274 (MQ=255)
                                                                                   gCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCCCCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCCCTGTACCGGTGCCCCGCCATTTGGCGCCTCCCAttg                                                                                                                                                                                                 >  2:785938/1‑274 (MQ=255)
                                                                                        gtCGGGCAAATCAAATCCAGCATGTTCCGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGTGATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGTTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGcc                                                                                                                                                                                            <  1:941895/273‑1 (MQ=255)
                                                                                                           gCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACgtggtt                                                                                                                                                                        >  1:865737/1‑274 (MQ=255)
                                                                                                           gCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACgtggtg                                                                                                                                                                        >  1:853636/1‑275 (MQ=255)
                                                                                                           gCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCAACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCACAGCCATTTGGTGCATCCCATTGCTGCCCTCTTTTCACCACCgtggtc                                                                                                                                                                        >  1:922842/1‑274 (MQ=255)
                                                                                                                ttCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCCGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCAACCTCTTTTTGCCCGTAGTTTGGTAATCCTTCTGTCGCGACCCACTGTACCGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTACCCGACGTGGTGTTCa                                                                                                                                                                    >  1:384265/1‑274 (MQ=255)
                                                                                                                tgaggCGTCACGCCATGTGTCCAGCCAAAGCCATCCTGTGATGTATATTCGCCACCGCCTCCCCCCGTTCCGTTTGTGCTGACAGCATATTTTTCCCCCAGCTTTTTCTCCCGGTCATAGTTGTGCTTAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCa                                                                                                                                                                    <  2:922841/270‑1 (MQ=255)
                                                                                                                     cGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgccgc                                                                                                                                                              <  2:358251/275‑1 (MQ=255)
                                                                                                                        cACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCCCTTTTCACCGACGTGGTGTTCAGGCCGCCgg                                                                                                                                                           >  1:408717/1‑275 (MQ=255)
                                                                                                                                     cAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCCGCAGAt                                                                                                                                               >  1:487910/1‑274 (MQ=255)
                                                                                                                                                  ccTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCACCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgc                                                                                                                                  <  1:1025166/274‑1 (MQ=255)
                                                                                                                                                           gATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTc                                                                                                                                                                                                                                             >  1:636607/1‑158 (MQ=255)
                                                                                                                                                             gatTCGCCACCGCCGCCCCCCGTTCCGGGGGTGCTGGCATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtt                                                                                                                        <  2:12695/272‑1 (MQ=255)
                                                                                                                                                               ttCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGa                                                                                                                                                                                                                                         <  2:636607/158‑1 (MQ=255)
                                                                                                                                                                tCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCCTTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCCGCAGATGGGTTTTCTTCGCCCTCCCCATTttgt                                                                                                                    >  2:594238/1‑274 (MQ=255)
                                                                                                                                                                tCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                                                                                    >  1:758385/1‑274 (MQ=255)
                                                                                                                                                                tCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTAATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCTCATCCCATTGTTGCCCACTTTTCACCGACCTGGTGTTCAGGCCGCCGGGTTTCAGCAGATGTGGTTTCGCTGCCGTCGCCAtttttt                                                                                                                    >  2:360930/1‑272 (MQ=255)
                                                                                                                                                                            ccccccGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCtt                                                                                                        >  1:760393/1‑274 (MQ=255)
                                                                                                                                                                                    tCCGGTGGTGCTGACATCATATTTTTCCACCCGCTTTTTCTCCCGGTCATAGGTGTGCTGAACCTTGGTCAGTAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTCGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                                                                                               <  1:989899/275‑1 (MQ=255)
                                                                                                                                                                                    tCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                                                                                               <  1:700785/275‑1 (MQ=255)
                                                                                                                                                                                    tCCGGTGGTGCTGACATCATATTTTGCCACCAGCTTTTTCTCCCGGTCATAGGTTTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                                                                                               <  1:936327/275‑1 (MQ=255)
                                                                                                                                                                                      cgggtgtgCTGACAGCATATTTTTCCACCAGCTTTTTCTACCGGTCATAGTGGTGCTGAACAGTGGTTAGGAAGTTCCAGCGAAGGTCCAGCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                                                                                             <  2:918791/270‑1 (MQ=255)
                                                                                                                                                                                      cGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                                                                                             <  2:758385/275‑1 (MQ=255)
                                                                                                                                                                                      cGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                                                                                             <  2:487910/275‑1 (MQ=255)
                                                                                                                                                                                      cGGTGGTGCTGACATCATATTTTTCCACCAGCTTGTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCa                                                                                             <  1:775023/275‑1 (MQ=255)
                                                                                                                                                                                           gtgCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTTGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTCGTTGGCGCGATCTTTCGCTGCCGCATTGAc                                                                                        >  2:77026/1‑275 (MQ=255)
                                                                                                                                                                                             gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCCCCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGTTTGCCGCCGTTGTGTTTTGCTCGCCTTCGCCATGTTGTTGGCGCGCTCTTTCGCTGTCGCATTGCCGt                                                                                      >  1:408181/1‑275 (MQ=255)
                                                                                                                                                                                             gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGt                                                                                      <  2:853635/275‑1 (MQ=255)
                                                                                                                                                                                             gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTGTTGTAATCCTTCTGTCGCGACCCACTGTAACTGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGt                                                                                      <  1:360930/275‑1 (MQ=255)
                                                                                                                                                                                              cTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCCCTGTAACGGTGCCCAGCCATTTTGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCCGATGTGTTTTCGTCGCCGTCGCCCTTTTGTTGGCGCGATCTTTCGCGTCCGCATTGACTTa                                                                                     >  2:240634/1‑275 (MQ=255)
                                                                                                                                                                                                                accaGCTTTTGCTCCCGGTCATAGGTGTTCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAAGCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcggc                                                                   <  1:62095/275‑1 (MQ=255)
                                                                                                                                                                                                                  caGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCCGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGACGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTATAGCGGGAAAAGGGCGGcc                                                                  >  1:86521/1‑274 (MQ=255)
                                                                                                                                                                                                                               cGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTTGCGCACCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTTCCGCATTGCCGTACATCGGGAACCGGGCGGCCGCGGTTCACTgc                                                      >  1:404085/1‑272 (MQ=255)
                                                                                                                                                                                                                                  tcagagtgtgtctggacagggttCAGGAAGTGCCAGCTAATGTCCATCGCCCCCTCTTTTGGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTTTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTgcg                                                 <  2:567140/253‑1 (MQ=255)
                                                                                                                                                                                                                                  tCATAGGTGGGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGGTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTgcg                                                 <  1:564007/275‑1 (MQ=255)
                                                                                                                                                                                                                                           tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCCTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGGTGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGCTCTTTCGCTGCCGCATTGACGTACCGCGGGAACAGGGGGGCCGCGGTTAACTGCTTGCGCCATTTATg                                        >  1:500693/1‑275 (MQ=255)
                                                                                                                                                                                                                                           tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTCACTGATTGCGCACtttcag                                        >  2:617391/1‑272 (MQ=255)
                                                                                                                                                                                                                                           tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTGTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTCGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCCCCTTTTTGTTGGCGCGCTCTTTCGCTGCCGCATTGACGTACACCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTCTg                                        >  2:1038415/1‑275 (MQ=255)
                                                                                                                                                                                                                                                  cATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATCTTGTTGGCGCGCTCTTTCGCTGCCGCATTGACGTACAGCGGGAACCGGGCGGCCGCGGTTACCTGAGTGCGCACTTTATGACCTTTc                                 >  2:296543/1‑275 (MQ=255)
                                                                                                                                                                                                                                                     tGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCt                                                                                                   >  1:435077/1‑206 (MQ=255)
                                                                                                                                                                                                                                                     tGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAg                               >  1:22854/1‑274 (MQ=255)
                                                                                                                                                                                                                                                         cAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                                                                                               <  2:435077/206‑1 (MQ=255)
                                                                                                                                                                                                                                                          agtaaGTCCCAGCTAATGTCCATCGCCCCCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTa                         <  1:474666/272‑1 (MQ=255)
                                                                                                                                                                                                                                                          aGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCCCGATCTTTCGCTGCCGCATTGACGTCCAGCGGGAACAGGGCGGCAGCGGTTAACTGATTGCGCACTTTTGGACTTTTAAGGCCGt                          >  2:788586/1‑274 (MQ=255)
                                                                                                                                                                                                                                                           ggAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTTTTTTCGTCGCCGTCGCCCTTTTGTTGGCGCGCTCTTTCGCTGCCGCATTGACGTACAGCGGGACCAGGGCGGCCGCGGTTAACTGCTTGCGCACTTTATGACTTTTCAGGTCGTa                         >  2:557129/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                 gCCAGCTAATGTCCATCGCCACCTCTTTTTGTCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGTCGCGATCTTTCGCTGCCGCCTTGACCTACAGCTGGAACAGGGCGCGCGCGGTTAACTGCTTGTGCACTTTATGACTTTTCAGGTGGTCATCgg                    >  1:723496/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                 gCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCCCTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCCCGGTTAACTGCTTGCGCACCTTATGACTTTTACGGTCGTAATCGGCa                  >  2:971712/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                 gCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTTCAGCGGGTACAGGGCGGTCGCGGTTACTTGCTTGCGAACTTTATGACTTTTCCGGTGGTACTCCGCa                  >  2:166783/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                 gCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGAt                                                     >  2:1041149/1‑240 (MQ=255)
                                                                                                                                                                                                                                                                    agttaattgccACCGCCACCTCGTGTTGCCCGTAGTTTTGTAATCCTGCTGTCGCGACCCACGGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATAc               <  2:865736/266‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTgcg                                                 <  1:1041150/240‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gCTAATGTCCATCGCCACCGCTTTTGGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATAc               <  1:324523/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       taacttCCATCGCCACGTCTTTTTGCCCGTAGTTTGGTAATCCTTCTGTCGCGACCCAGTGTAACGGTGCACAGCCATTGTGCGCATCCCATTGTTGCCCACTGTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAg            <  1:743111/270‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tAAGGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCGGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCa             <  2:1022082/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       caaTGTCCAACGCCACCTCTGTTTGCCCGTATGTTTGTACTCCTTCTGTCGCGACCCCCTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCa             <  2:384265/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                          tGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCCTCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCTATCTTTCGCTGCCGCATTGACGTACCGCGGGAACAGGTCGGCGGCGGTTGACTGATTGCGTCCTTTATGACTTTTGAGGTCGTAATCGGTCTACCAGcc          >  2:689964/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                               aTCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCttgttg    >  1:842001/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                               aTCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCttgtt     <  1:785938/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                tCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCCTCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGCACAGGGCGGCCGCTGTTTACTGATTGCTCACTTTATGCCTTTTCAGGTCCTAATCCGCCTACCAGCCttgttgt   >  2:74763/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                tCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggc                                                                      >  2:1037971/1‑208 (MQ=255)
                                                                                                                                                                                                                                                                                tCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGAGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCCTTGACGTACAGCGGGAACAGGGCGCCCGCGCTTAACTGATTGCGCACTTTATGACTTTTCGGGGCGTAATCGGCACACCAGCCTCTTTGa   >  2:737511/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                 cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGCTTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCttgt      >  1:956898/1‑271 (MQ=255)
                                                                                                                                                                                                                                                                                 cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCTTGTTGAt  >  1:263308/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                 cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCTTGTTGAt  <  1:788586/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cGCCACCTCGTTTTGGCCGTAGTTTGGTAATCCTTCGGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCACCCCATTGTTGCCCACTTTTCACCGACGTGGGGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCTTGTTGAt  <  2:404085/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                 
TGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTAACGGTCGGACGCGTCGCCGGAACATTGTCACACGGTTGCTCTTTCGGGCAGATCAAATCCAGCATTTTCAGCGTCACGCCATTGGTCCAGCCAAAGCCATCCTGTAATGGATATTCGCCACCGCCGCCCCCCGTTCCGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCCGCGGTTAACTGATTGCGCACTTTATGACTTTTCAGGTCGTAATCGGCATACCAGCCTTGTTGAT  >  NC_000913/1245685‑1246232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑