Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,354,909 A→T 7.4% intergenic (‑191/+46) lysU ← / ← dtpC lysine tRNA synthetase, inducible/dipeptide and tripeptide permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,354,9090AT 7.4% 172.3 / 2.6 50intergenic (‑191/+46)lysU/dtpClysine tRNA synthetase, inducible/dipeptide and tripeptide permease
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base T (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GCTGACGTAGTGCCGCCAGTTTTTCGCGGCGATTTCTCAGTTCATCGTTAAAATCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGTTGCCAGCATATAAATCCCGGTTAATACGCC  >  NC_000913/4354625‑4355197
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                
gCTGACGTAGTGCCGCCAGTTTTTCGCGGCGATTTCTCAGTTCATCGTTAAAATCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATaaa                                                                                                                                                                                                                                                                                              >  2:982071/1‑289 (MQ=255)
        aGTGCCGCCAGTTTTTCGCGGCGATTTCTCAGTTCATCGTTAAAATCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAg                                                                                                                                                                                                                                                                                       <  2:806389/288‑1 (MQ=255)
                    ttttCGCGGCGATTTCTCAGTTCATCGTTAAAATCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTc                                                                                                                                                                                                                                                                            <  1:982071/287‑1 (MQ=255)
                           ggcgATTTCTCAGTTCATCGTTAAAATCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCaa                                                                                                                                                                                                                                                                    <  2:1008029/288‑1 (MQ=255)
                                                     tCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTGCAGACATTTTGGTTACTCTAAATCCAGCTTTTAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATATGCCTCGACTATTAGTAACTCAGTATATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCATGATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTATTACGACAGTTAATCGTTGct                                                                                                                                                                                                                                         <  2:831424/289‑1 (MQ=255)
                                                                         ccGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATtcgtcg                                                                                                                                                                                                                     <  1:864851/289‑1 (MQ=255)
                                                                         ccGTGTTTCTGGTTCCGACATGTGGGTTCCGCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCGCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTGAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATtcgtcg                                                                                                                                                                                                                     <  2:528664/289‑1 (MQ=255)
                                                                              tttCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTACCATATTCGTCGGCGt                                                                                                                                                                                                                 >  2:1131429/1‑288 (MQ=255)
                                                                                           cATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATACTCGTCGGCGTGCTAAACAGaaa                                                                                                                                                                                                     <  2:306859/287‑1 (MQ=255)
                                                                                                            aTCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAAt                                                                                                                                                                                  <  1:527149/289‑1 (MQ=255)
                                                                                                                aGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAACCAGAAAACGGGTAGCAAAGGCCAATacg                                                                                                                                                                               >  2:909586/1‑288 (MQ=255)
                                                                                                                         tttAGCGTAGTAAAAGACACAGATCTGTACACGTTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAGCCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATcg                                                                                                                                                                     <  2:579719/289‑1 (MQ=255)
                                                                                                                                    tAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAGCTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGcc                                                                                                                                                          <  2:662302/289‑1 (MQ=255)
                                                                                                                                               aTCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCAct                                                                                                                                                >  2:1009530/1‑288 (MQ=255)
                                                                                                                                                tCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCActc                                                                                                                                               <  1:1124773/288‑1 (MQ=255)
                                                                                                                                                           tattatCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCGATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTgaga                                                                                                                                   >  2:711425/1‑289 (MQ=255)
                                                                                                                                                           tattatCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCa                                                                                                                                                                                           >  1:1098080/1‑233 (MQ=255)
                                                                                                                                                           tattatCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCa                                                                                                                                                                                           <  2:1098080/233‑1 (MQ=255)
                                                                                                                                                                     cTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAGGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGACAAGAATCg                                                                                                                          >  2:897294/1‑288 (MQ=255)
                                                                                                                                                                     cTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATGCGACGATGATCGCGACACAAGCCAACGTCCACTCCCCCATCTGAGAAAAGAATc                                                                                                                           >  2:1072555/1‑287 (MQ=255)
                                                                                                                                                                     cTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCGCTCTCCCATCTGAGAAAAGAATc                                                                                                                           >  2:689271/1‑287 (MQ=255)
                                                                                                                                                                     cTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATc                                                                                                                           >  1:1042819/1‑287 (MQ=255)
                                                                                                                                                                     cTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATc                                                                                                                           >  1:793338/1‑287 (MQ=255)
                                                                                                                                                                     cTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCg                                                                                                                          >  1:532935/1‑288 (MQ=255)
                                                                                                                                                                                   aCTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTg                                                                                                                                                                                                                <  2:1221549/188‑1 (MQ=255)
                                                                                                                                                                                   aCTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTg                                                                                                                                                                                                                >  1:1221549/1‑188 (MQ=255)
                                                                                                                                                                                    cTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTTTAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAAtt                                                                                                          >  1:897677/1‑289 (MQ=255)
                                                                                                                                                                                    cTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAAtt                                                                                                          >  2:704980/1‑289 (MQ=255)
                                                                                                                                                                                         tttATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCAt                                                                                                     >  1:23385/1‑289 (MQ=255)
                                                                                                                                                                                         tttATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCAt                                                                                                     >  2:84988/1‑289 (MQ=255)
                                                                                                                                                                                                 atcGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTaa                                                                                             >  2:807521/1‑289 (MQ=255)
                                                                                                                                                                                                       aCTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTg                                                                                         >  1:514195/1‑287 (MQ=255)
                                                                                                                                                                                                       aCTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTg                                                                                         <  2:514195/287‑1 (MQ=255)
                                                                                                                                                                                                          aaCGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGAct                                                                                    <  2:1002090/289‑1 (MQ=255)
                                                                                                                                                                                                                 aCCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACCCTgtcg                                                                              >  2:1131436/1‑288 (MQ=255)
                                                                                                                                                                                                                                        aatacCTATCAAAAAGCGTTCAATAACTTTTTAAGTTCAGTTGGTAAGGGTTTTAAACGGAGATGTAATTTATTCATAACCAATTGGTTTGACGACAGTTAATCGTTCCTCTCCTGTATCATATTCGGAGGCGGGCTAAACAGAAACCGGGAACCAAAGGCCAATACGACGATGATCGCAACACAAGCCAACGTCCACGCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGGCGTCTGCTGTGCCACGACGCCTgcca                                                      <  2:829299/289‑1 (MQ=255)
                                                                                                                                                                                                                                           aaCCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTgccagcc                                                   >  1:770394/1‑289 (MQ=255)
                                                                                                                                                                                                                                                tCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTTTAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAAc                                                                                                                                                                                                          >  1:143524/1‑133 (MQ=255)
                                                                                                                                                                                                                                                tCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTTTAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAAc                                                                                                                                                                                                          <  2:143524/133‑1 (MQ=255)
                                                                                                                                                                                                                                                tCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCa                                                                                                                                                                                           >  1:943994/1‑148 (MQ=255)
                                                                                                                                                                                                                                                tCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCa                                                                                                                                                                                           <  2:943994/148‑1 (MQ=255)
                                                                                                                                                                                                                                                 cAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCCGGTAAGCTGCAATTGCCATACCGCTAATTTGCAACTCTGTCGTCTGCTGTGCCACGACCCCGCCCAGCCAGTTgg                                             >  2:430359/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        gTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACcg                                       >  2:27301/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    tattaATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCGCTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGTTGCCAg                          <  1:689271/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGTTGCCAGCATATAAAt                 <  1:242098/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              gATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGTTGCCAGCATATAAATc                <  1:1009530/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aTTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGTTGCCAGCATATAAATc                <  2:23385/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   gTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGt                                <  1:940870/268‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   gTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGt                                >  2:940870/1‑268 (MQ=255)
                                                                                                                                                                                                                                                                                    ttAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTGTCCCATCTGAGAAAAGAATCGCTGGTACGCTGCAATTGCCACACCGCTAATTTGAGACCATGACGTCTGCTGTGCCACGACGACTGCCAGCACGTTGGCGCCAGCGCCTGTTGACAGCCTACAAAACCCGGtt          >  2:757090/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            ataAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGTTGCCAGCATATAAATCCCGGTTAATACGcc  >  1:656452/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                
GCTGACGTAGTGCCGCCAGTTTTTCGCGGCGATTTCTCAGTTCATCGTTAAAATCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTTCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATAAAGAGACAGATCGGTACACTGTATTATCTGCCTCGACTATTAGTAACTCAGTTTATCTTATCGAAACTAACGCTTACCTCAAGTTGATATCACTAATAAATAACCATCAAAATGGTTTAAATGACTTATTAATCTCGATTTGTTAGTGTTATAAACTGAAAGTTAATTTATTCATAACCAATTGTTTTTACGACAGTTAATCGTTGCTCTCCTGTATCATATTCGTCGGCGTGCTAAACAGAAAACGGGTAGCAAAGGCCAATACGACGATGATCGCGACACAAGCCAACGTCCACTCTCCCATCTGAGAAAAGAATCGCTGGTAAGCTGCAATTGCCATACCGCTAATTTGCGACTCTGTCGTCTGCTGTGCCACGACGCCTGCCAGCCAGTTGGCGACCGCGCCTGTTGCCAGCATATAAATCCCGGTTAATACGCC  >  NC_000913/4354625‑4355197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: