New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 62 (0.970) | 13 (0.230) | 12/252 | 4.6 | 19.3% | coding (290/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | 1209619 = | 54 (0.950) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
Rejected: Coverage evenness skew score above cutoff. | |||||||||||
Rejected: Frequency below/above cutoff threshold. |
TCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTG > NC_000913/1209478‑1209757 | tCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAAACGGGACACCAACAGGTAATGCAGAGACTTCTCCCAAACCAACg > 2:160912/1‑143 (MQ=255) ggagcaGCCTGTGCAAGTTCCGGGTATTCTTCAGCAGTCAAAGAGGCACAATTCCCTTTCAGCCAGCATGTTGGCGGAGTGGCTGAAGGCCACGGAAAAGGGATCCGCAAACGGCATGCAGAGCCTTCTCCCAAACCAACGtt < 1:850077/138‑1 (MQ=255) aaGCCTGTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGCCCCAAATAGGTTATGCAGAGCCTTCTCCCAAACCAACGTTTATg < 2:1066783/143‑1 (MQ=255) aGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCAGAGTGGCTGAAGGCACCGGAACCGGGACACCAACAAGTAATGCAGAGCCTCATCCCAAACCAAccaataaga > 1:405342/1‑135 (MQ=255) ccTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGaaa > 1:829302/1‑143 (MQ=255) tttGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCGAACCAAGGttttcgagcgc > 2:1037164/1‑135 (MQ=255) gCCCGTTCCTGTTATTCTTCAGCAGAGAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATgaa < 1:987378/143‑1 (MQ=255) gCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACACGTAGTGCAGCGCCTACGCCCAGACCAAGGtcctcgacacccgc > 1:5659/1‑130 (MQ=255) gCCAGTGCCGGGTATGCTTGAGCAGAAAAAGCGGCACCAGTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATgaa < 1:990403/143‑1 (MQ=255) ccGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATaa > 2:999520/1‑143 (MQ=255) gcgTATTCTGCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGTAGGCCACTGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACa < 2:187085/141‑1 (MQ=255) ttcAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGCAGAAATAACAAGCaaacg > 2:1120506/1‑141 (MQ=255) cagAAAAAGCCGCAGCATCGCATATCAGCCAGCCTGGTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACAACAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGcatc < 2:21439/143‑1 (MQ=255) gAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGCCCACGGAACCGGGACGCCGACAGGTACTGCAGAGCCTGCTCCCAAACCAACGTCTATGAAAATGAAGAAAGAACAAGGAAATCGcatcag > 1:607208/1‑142 (MQ=255) aaaaaGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGcctcaat > 2:351282/1‑141 (MQ=255) aaaaaGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCAtt > 2:1097447/1‑143 (MQ=255) aaaGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAGACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGcatcagccc > 2:930182/1‑139 (MQ=255) acgCGGCACCTTCGCTTGTTATCCCGCCTGTTGGCGGAGTTGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCt < 1:528692/141‑1 (MQ=255) aGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCGACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTg < 1:776401/143‑1 (MQ=255) gCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGc < 2:974860/143‑1 (MQ=255) cGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCt < 1:116371/143‑1 (MQ=255) cACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCtttt < 1:1086900/143‑1 (MQ=255) aCCATTGCATTTCAGCCAGCCTGTTGGCGGCGTGGCTGAAGGCCACGGCACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTa > 2:883097/1‑143 (MQ=255) ttGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCCAACCAACGCGTACGAACATGAAGAAATAACACGCAAATGGCATCACGCCTGCTTATACCAg > 1:961447/1‑143 (MQ=255) tttCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGa > 2:1022306/1‑143 (MQ=255) tggccggggggggggCGGAGTGGCGGAAGGCCAGGGAACCGGGACACCAAGAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAAGAACAAGCAAATGGCATCATTCCTGGTTTTACCAGGGGGAttg < 2:407642/130‑2 (MQ=255) ccagccTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGCACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCAGCATTCCTGCTTTTACCAGGGGGATTTAAc > 1:512386/1‑143 (MQ=255) agcgTGTTGGCGGAGGGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACAt < 2:1015997/143‑1 (MQ=255) ccTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGc < 2:302620/143‑1 (MQ=255) cTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTGAACATGcg > 1:696374/1‑142 (MQ=255) gCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTgg < 2:829302/143‑1 (MQ=255) tGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTACGGATATGCAGAGATAAAAGGAAAAACATCTCGCTCCGTCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTAcgc < 2:1116522/143‑1 (MQ=255) tGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGCTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAGCATGCTTATAGGCTATCTAcgc > 2:429794/1‑143 (MQ=255) gAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTAcgcg > 2:790947/1‑143 (MQ=255) agggCCACGGAGCCGGGAGACCAACAGGTAATGCAGAGCCTTCTCCCAGACCAACGTTTATGAAAATGAAGAAGTGACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGt < 1:1120506/141‑1 (MQ=255) gcggcgaCCGGGACAGCAACAGGTAATGCAGAGCTTTCTCCCCAAGCAACGTTGATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATcaa < 1:484138/137‑1 (MQ=255) cACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATg > 1:1048874/1‑131 (MQ=255) cACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATg < 2:1048874/131‑1 (MQ=255) ggAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGCTTTAACATGCTTATTGGCTATGTACTCGTATcaacaa > 2:951548/1‑143 (MQ=255) ggAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAAGGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTTTTGGCTATGTAGGCGTATcaacaa > 2:209033/1‑143 (MQ=255) cGGGACACAAACAGGTAATGCAGAGACTTCTCCCAAACCAACGATTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCATGCTTTTACTAGGGGGATTTAACATGCTTATTGGGTTTGTACGCGTATCAACAGATGAc > 2:1029777/1‑143 (MQ=255) gggACCCCAACAGGGAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGAcc < 2:1056782/143‑1 (MQ=255) cacCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATAGCATCATTCTTACTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACTAATGACCAGaa > 1:908658/1‑143 (MQ=255) aaCAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCCACAAATGACCAGAGcaca > 2:670148/1‑143 (MQ=255) aaCAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAAcaca > 2:829724/1‑143 (MQ=255) aGGTAATGCAGAGCTTTCTCACAAACCAACGTGTATGAAAATGATGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGAt < 1:3932/143‑1 (MQ=255) aGGTAATGCAGAGCCTTCTCCGAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGAt < 1:244155/143‑1 (MQ=255) aGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGAt > 2:662302/1‑143 (MQ=255) gTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCt > 2:886118/1‑143 (MQ=255) aaTGCAGAGCCTTCTCCCAAACCGACGTTTATTAAAATTAAGAGATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTAc < 1:457143/143‑1 (MQ=255) tGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATACACAAATGACCAGAACAGAGATCGACaa > 1:477778/1‑143 (MQ=255) gCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCAGCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCCACAAATGACCAGAACACAGATCTACAAc > 1:556394/1‑143 (MQ=255) cAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACg > 2:14754/1‑143 (MQ=255) agagCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAACCAAATCGCGTCACTCCAGCTATTAAAAGGTGGATTTAACATGCTTATTGCCTATGTACGCGTATCAACAAATGACCGGAACGCAGCTCTACAACgc > 1:236096/1‑142 (MQ=255) ccTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATg > 1:912529/1‑143 (MQ=255) ttCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATTTTTATTGGCTATGTACTCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATgcg > 2:414885/1‑143 (MQ=255) ttCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATgcg > 2:678944/1‑143 (MQ=255) cccAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGa > 2:1062399/1‑143 (MQ=255) ccAAACCAACGTTTATGAAAATGAAGCAATAACAAGCAAATGGCATCATTCCTGCTTTTGCCAGGGGGATGTAACATGCTTATTGGCTATGTACGCGTATCGACAACTGACCAGAACACAGATCTACAACGTAATGCGCTgcg > 1:425912/1‑141 (MQ=255) ccAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCCTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGaa < 2:634991/143‑1 (MQ=255) ccAAACCAACGTTTATGAAAATGAAGAAATAACAAGAAATTTACAGCATGCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGaa < 1:964583/143‑1 (MQ=255) cAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAAc < 2:912529/143‑1 (MQ=255) aaaCCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACt > 2:817226/1‑143 (MQ=255) ccAACGTTTCTGCACCTGGGGAACTAACAAGCAAATGGCATCAGTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCTGAACACAGATCTACAACGTAATGCGCTGAACtgtg < 1:268791/143‑1 (MQ=255) ccAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATCCTTATTACCTCTGTACGCGTATCAGTAAATGACCCCACCACAGATCTACAACCCAATGCCCAGGACtgag > 1:432500/1‑141 (MQ=255) | TCGGATAAGCCTTTGCCAGTTCCGGGTATTCTTCAGCAGAAAAAGCGGCACCATTGCATTTCAGCCAGCCTGTTGGCGGAGTGGCTGAAGGCCACGGAACCGGGACACCAACAGGTAATGCAGAGCCTTCTCCCAAACCAACGTTTATGAAAATGAAGAAATAACAAGCAAATGGCATCATTCCTGCTTTTACCAGGGGGATTTAACATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAACACAGATCTACAACGTAATGCGCTGAACTGTG > NC_000913/1209478‑1209757 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |