Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,2460GT30.0% 44.1 / 16.2 40A53E (GCG→GAG) ynjFCDP‑alcohol phosphatidyltransferase family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (14/14);  new base T (5/7);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAACAACGGTTTAATCCGGGGA  >  NC_000913/1841107‑1841383
                                                                                                                                           |                                                                                                                                         
gCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCTCCa                                                                                                                                        <  1:293114/143‑1 (MQ=255)
  cAAACGGCACCAGCGAGTAAAAGAGAAAACCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGa                                                                                                                                      <  2:826171/143‑1 (MQ=255)
             aGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGGCAGATAccagcccag                                                                                                                           >  2:275179/1‑143 (MQ=255)
               cgcgTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGAGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGTCGATCAAGCAGCCTGTACACCAAAATGACTACTAGCGCCGCGAGATaaccacacagcg                                                                                                                         >  1:280494/1‑132 (MQ=255)
                    aaaaGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGa                                                                                                                    >  1:108414/1‑143 (MQ=255)
                          gaAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGc                                                                                                              <  2:338563/143‑1 (MQ=255)
                             caTCGGGAGAAATATCAAGAACGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCCGTTCAACAAACTGACGACTAGCGCCGCCAGATACCACCCCAGCGCCAGACACGCCaga                                                                                                           >  1:288557/2‑143 (MQ=255)
                             attctggagaAATATCAAGAAAGCCCCCCTCCTCGGTATGCTTTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAGAATGACGACGAGCGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCaga                                                                                                           <  1:697883/137‑1 (MQ=255)
                                   gagaAACACCAAGAGACGCGCGCGCGTCGGTAAGCTCGCCGCGCCGCGCCAGCGCACCGTCCAGACCATCACGCAGCCTGTTCAACAAAATGACGACTAGCGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGc                                                                                                     <  2:492947/143‑1 (MQ=255)
                                   gagaAAAATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGc                                                                                                     <  2:610967/143‑1 (MQ=255)
                                          atCAAGATAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGTGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGa                                                                                              <  1:325804/143‑1 (MQ=255)
                                           tCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACACATTGCCGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGAt                                                                                             <  2:886198/143‑1 (MQ=255)
                                            gaaGAAAGCCGCCCGCGGCGGGAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGGCGGTTCAACAAAATGACGACTAGCGCCGGCAGATACCAGCCGAGCGCCAGAAACGGCAGAGCCAGCACGCCGATg                                                                                            <  2:498501/142‑1 (MQ=255)
                                                  aGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGCGCCAGCACGCCGATGGCaaac                                                                                      >  2:457075/1‑142 (MQ=255)
                                                             tCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGt                                                                           >  2:797468/1‑143 (MQ=255)
                                                                gTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGGTCAACAAAATGACGACTAGCGCCGCGAGATACCAGCCCAGCGCCCGGGACGGCCGAGCCAGCACGCCGATGGCAGATCGCACGAATGTTaa                                                                        >  2:872171/1‑143 (MQ=255)
                                                                  aaGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCTCCAGATACCAGCCCAGCGCCAGAGACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAAc                                                                      >  2:101696/1‑143 (MQ=255)
                                                                         ctGCGCCGCGCCAGCGCACCGTCCAGACCACCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCTGAGACCAGCCCAGCGCCAGAACCGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTgg                                                               <  2:942470/143‑1 (MQ=255)
                                                                              ccgctccAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGTGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCAGATCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTaa                                                          <  1:11379/143‑1 (MQ=255)
                                                                                gcgccAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGCCTAGCGCCGCCAAATACCATCCCAGAGCCAGAAACGGCAGAGCCAGCACGCCGCTGGCAAATCCCGCCAATGTAAAACCATCGGGCggagtg                                                        >  1:183810/1‑138 (MQ=255)
                                                                                gcgccAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGACACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCACACCCCACTAACGCTAAACCATCTGGCggacag                                                        >  1:587610/1‑138 (MQ=255)
                                                                                   ccAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGccc                                                     <  2:775029/143‑1 (MQ=255)
                                                                                     aGCGCACTGCCCAGACCATCAAGCAGCGTGTTCAACAAGAGGGCGACTAGCGCCGCCAGATCCCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCgg                                                   <  2:291385/143‑1 (MQ=255)
                                                                                       cgcaccgcacAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGtt                                                 <  2:1102400/143‑1 (MQ=255)
                                                                                        gcACCGTCCAGACCATCAAGCAGCCTGTTCCACAGTGGTAGAACTAGCGCCGCCAGAGACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGttt                                                <  1:1084027/143‑1 (MQ=255)
                                                                                          aCCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTAt                                              >  2:366669/1‑143 (MQ=255)
                                                                                          aCCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCACCAGACACGGCAGAGCCAGCACGCCGACGGCACATCCCACTCATGCTACACCCTCTGGCGTAATGCCCgcgtccg                                              >  1:375112/1‑137 (MQ=255)
                                                                                              tCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAg                                          >  1:854884/1‑143 (MQ=255)
                                                                                                 aGACCACCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCCGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAAc                                       <  1:387610/143‑1 (MQ=255)
                                                                                                             agcCTGTTCCACAAAATGACGACGAGCGCCTCC‑GATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGa                          <  1:154234/143‑1 (MQ=255)
                                                                                                                cggttgcacaaAATGACGACTAGCGCCGCCACATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAAGCCCAATAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATg                        <  1:691249/136‑1 (MQ=255)
                                                                                                                cTGTTCAACAAAATGACGACTAGCGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATg                        <  2:604982/143‑1 (MQ=255)
                                                                                                                         aaaaTGACGACTAGCGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAACAACgg               >  2:1114351/1‑143 (MQ=255)
                                                                                                                         aaaaTGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAACAACgg               <  2:108414/143‑1 (MQ=255)
                                                                                                                              gacgacTAGCGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACACACTGAGGAACCAAAGGTTTaa          >  1:508156/1‑143 (MQ=255)
                                                                                                                                  acTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGCTTATCAAGAACCCGCACGCACTGATGCAACAACGGTTTAATCCg      >  1:510166/1‑143 (MQ=255)
                                                                                                                                    tAGCGCCTCCGGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAACAACGGTTTAATCCggg    <  1:795092/143‑1 (MQ=255)
                                                                                                                                    tAGCGCCTCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCCATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAACAAGGGTTTAATCCggc    >  1:212888/1‑142 (MQ=255)
                                                                                                                                      gcgcCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAACAACGGTTTAATCCGGGGa  >  2:906929/1‑143 (MQ=255)
                                                                                                                                      gcgcCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAAACACGGTTTAATCCGGGGa  >  2:554277/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAGCTCTCTGCGCCGCGCCAGCGCACCGTCCAGACCATCAAGCAGCCTGTTCAACAAAATGACGACTAGCGCCGCCAGATACCAGCCCAGCGCCAGAAACGGCAGAGCCAGCACGCCGATGGCAAATCCCACTAATGTTAAACCATCTGGCGTAATGCCCGGTTTATCAAGAACCCGCACGCACTGATGCAACAACGGTTTAATCCGGGGA  >  NC_000913/1841107‑1841383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑