| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,361,533 | 0 | A | T | 20.4% | 82.2 / 12.3 | 46 | I226N (ATC→AAC) | rhmR | putative DNA‑binding transcriptional regulator for the rhm operon |
| Reads supporting (aligned to +/- strand): ref base A (17/18); new base T (0/9); total (18/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTC > NC_000913/2361393‑2361675 | ttAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGAtt < 1:901160/143‑1 (MQ=255) tAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGAttt > 2:1115494/1‑143 (MQ=255) aGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTa < 2:139120/143‑1 (MQ=255) cATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCCGAGGGGCTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGac < 2:566195/143‑1 (MQ=255) cATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGac < 2:556230/143‑1 (MQ=255) cATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGac < 2:111929/143‑1 (MQ=255) atgatgTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCACTGGAGCAGGCAATGGCCTTGCCCGCCAGATAC‑TCACGGGCATCTGCATGGCTTTGTACGCGAGTAC‑‑CGacg > 1:539074/1‑142 (MQ=255) gatgTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGaccac < 2:1023918/143‑1 (MQ=255) ctcCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAt < 2:388659/143‑1 (MQ=255) ccATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCg > 2:523489/1‑143 (MQ=255) ccATTGTTATGAGGCTTGTAAGTCAAAGCGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCg < 2:893411/143‑1 (MQ=255) cATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGc > 1:1118246/1‑143 (MQ=255) tgtgATGAGGGTTGTAAGTCAAAGGGACTTTTCCATCCCAACGGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGc < 1:808797/143‑1 (MQ=255) gAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTGCATTGATGCGTAAACGGGTAC‑‑TGACCACCGAGATCGCAGCGGTAAg > 2:150511/1‑143 (MQ=255) ttGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTCAGCTCgc > 2:17290/1‑142 (MQ=255) tgcacgTCAAATGGTCTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCATTTTATGGCCTTGCCCGCCGGATAC‑TCACGGTATTCTTCTTTTTTTTGTAA‑‑TGATACGGCGACCACCGAGATCGCAGCGGTAAGCTCGtt < 1:810798/138‑1 (MQ=255) tAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCTGCTGGTTAA‑TCCAGGTCTTCTTCATTTATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTAt < 2:819410/143‑1 (MQ=255) aaGTCAAAGGGACCTCTCCGTCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTAtt < 2:692845/143‑1 (MQ=255) aGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCCCGTTATTg > 2:604160/1‑143 (MQ=255) tCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCCCCGACATCGCCCCGGTAAGCTCCTTATTGGc > 2:1114965/1‑143 (MQ=255) tgctgttcTTTTCCATCCCTACATGCGTGAAGTCTCCGTGGGGCAGGTTATGGTTTTGCCCGCCAGATCA‑TCACGGTGTGCTTCTTTTTTTTTTAA‑‑TGATACGGCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGt < 1:861693/136‑1 (MQ=255) atagggacgcctccctcccAACAGGCGTGATATAGCCCCGGCGCAGGCAATGGCCGTGCCCGCCAGATAA‑TCTCGGTATTCTTCATTGCTTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGt < 2:500286/132‑1 (MQ=255) aaaGGTACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGt < 2:398127/143‑1 (MQ=255) aGGGACTTTTCCACCCCAACAGACGTGAAACATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTa < 2:593292/143‑1 (MQ=255) ggtggttttCCAGCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCATTATTTGTTAGATAG‑TCACGGTATTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTaa < 1:389599/138‑1 (MQ=255) ggtaCTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCTTTGTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTaa < 1:197413/140‑1 (MQ=255) ttttCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACAcc > 2:899118/1‑143 (MQ=255) tcttacagcccAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCCTTGATTGGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCGCGCTATTAGCGTTaacaacc > 1:552051/9‑138 (MQ=255) ccAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGCGATCGCCGCGGCAAGCTCGTTATTGGCGCTCCACCCCGGCGCAGCg > 2:835092/1‑143 (MQ=255) taaCTGAGGTGCAATAGCGCTAGCGCTGGCCATTGCCTTGCCCGCGAGGTAG‑TCACGGTATTGTTCTTTTTTTTTTAATCATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGa < 1:746095/142‑1 (MQ=255) tGAAATATCCCTGGCGCAGGCAATGGCCTTGACCG‑CTGTTAATTCTCTTTTTTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACGCAACGGa > 2:768127/1‑143 (MQ=255) tgcgcAGGCATTGTTCTTGCCCGGCAGCTAG‑TCACGGTATTGTTCTTTTTTTTTTAAGGATGCGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAAt < 1:710322/142‑1 (MQ=255) ggCGATGGGCTTGCCAGCCAGAGAA‑TGACGGTATTCTTCGTTGATTTGGAAGGGGGCGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGc < 1:1027924/143‑1 (MQ=255) cAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCg > 2:714404/1‑143 (MQ=255) aaTGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACCACGGGCAGCGGCGGGATCGTCGcccg > 2:132260/1‑141 (MQ=255) cTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCg < 2:158367/88‑1 (MQ=255) cTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCg > 1:158367/1‑88 (MQ=255) cTTGCCAACCAGATAA‑TCACGGTATTTTTCATTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCAt < 1:464014/143‑1 (MQ=255) ccGCCAGCTAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTc < 2:356295/143‑1 (MQ=255) ccGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTc < 2:582892/143‑1 (MQ=255) cAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCCTTAAACACCGGCGAACAGACACAACGGACATCTGCGTAACCTCCGCCGATGGCAAAgacacagc > 1:902389/1‑136 (MQ=255) aGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACCTCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGcc > 2:500412/1‑143 (MQ=255) tATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGCCGTTAAACACCGCCGCAGCGACACAAGGGACAGCGGCGTAATCTTCGCCGCTGTCCTAGCTTCAGCCCTGGCGGCGGAt > 1:240786/1‑143 (MQ=255) tctttttGTTTTGTAAGGAGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATAcgc < 1:22334/138‑1 (MQ=255) cttcttTGATTTGTAAGCGTGTAG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATAcgc < 1:150511/143‑1 (MQ=255) tcATTGATCTGTAAGCGGGTAC‑‑CGACCACCGAGCTCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACgcgc < 2:142868/143‑1 (MQ=255) aTTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTGGTAGCTCCAGCGCTGGCGGCGAATAGGGGCCAGttc > 2:914603/1‑143 (MQ=255) | TTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAA‑TCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTC > NC_000913/2361393‑2361675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |