| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,913,766 | 0 | C | A | 20.0% | 66.4 / 10.9 | 42 | intergenic (‑99/+63) | glmS/glmU | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase/fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (19/13); new base A (5/3); total (25/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCG > NC_000913/3913624‑3913907 | tcttctACATCCCGTTGCTCGATCGCTCCATCCATTCCGCACCTATTTTTCGTTTCCGATTTTTGTCGCTGTCGGTCATCCTGTTTGCCCGTTTTTTCGTGCCCCCCGAGCCTTGTCGAGAGTGGGGTTATTTTTATAGTTAc < 1:166626/143‑1 (MQ=255)tCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTAc < 1:35652/143‑1 (MQ=255) gCTACATCACGTTTCGCGATCGCGCCAACCATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGc < 1:464894/143‑1 (MQ=255) gcgATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTCTTTTTATAGTTACTGCTTGTGGGA‑GGGAGAt > 2:636850/1‑143 (MQ=255) ctggcgcCATCAATTCCACACATAGTTTTTGCTTCCGATTTATATCGGTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTAt < 1:215366/140‑1 (MQ=255) aTCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTAt > 2:393655/1‑143 (MQ=255) tCGCTCCAACAACTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATg < 1:459268/143‑1 (MQ=255) aacaaTTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTCGAGAGTTGGGTTATTTTTATCGTTACTGCTTGTTGGA‑GGGGGATTATGTTAtctc > 2:621088/1‑143 (MQ=255) acacaTAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTAATGCTTGTGGGA‑GGGAGAGTATGTTATCTCCTCATCCCa > 2:773376/1‑143 (MQ=255) ccGACTTACATCGTTGCCGCCCAACCCGTAGGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCg < 2:144580/143‑1 (MQ=255) tgtCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTAATGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGTCAGAAt < 1:979768/141‑1 (MQ=255) tatCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGGTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGaaa > 2:952900/1‑142 (MQ=255) tCGTTGTCGGTCAACCTGTATGCCCGTTTTTTTTTTTTTTTTTAGCCTTGTAGAGGGCGGGGTTATTTTTTTTGTTATTGCTTGTGGGA‑GGGTGATTATGTTCTCTCCTCATCCCATGTGTCCGGGTTATCCGGCCGGACTct > 1:1000600/1‑142 (MQ=255) aaCCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCgg < 2:1117799/122‑1 (MQ=255) aaCCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCgg > 1:1117799/1‑122 (MQ=255) ccTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTCTGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCCCTTTTTCTTTACCg > 1:1075262/1‑143 (MQ=255) cccGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTAATGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTGCCCGCACGACGc > 2:245253/1‑143 (MQ=255) cctttttttCGGGCGCCCCGTGCCTTGTAGAGAGTGGGGTTATTTTTATAGTCACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGCCCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGcc < 1:1121102/140‑1 (MQ=255) ccGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGcc > 2:773711/1‑143 (MQ=255) cGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCa > 2:915860/1‑143 (MQ=255) gTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTGTTATAGTTACTGCTTGTGGGA‑GGGGGATGATGTTATCGCCTCATCCCATGTGACCGTGTTAGCCGGCCGAGATCACGTCTTCTGTACGCCGCGACGCCAg > 1:122496/1‑143 (MQ=255) ttttttCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTGTTCTTTACCGGACGACGCCAGc > 2:682587/1‑143 (MQ=255) tttCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGGTTATGTTATCTCCTCATCCCATGTGTCCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGCCGGCGCCAGCctt > 1:712001/1‑143 (MQ=255) gggCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTAATGCTTGTGGGA‑GGGAGAGTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCCGAATCACATTTGCTTTACCGGACGACGCAAGccagcatt > 1:956571/1‑137 (MQ=255) gcgcCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCttctttct > 2:46106/1‑143 (MQ=255) gcgcCCCGAGCCTGGTAGAGAGTGGGGTTAGTTTTATAGTTACTGCTTGTGGGG‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCttctttct < 1:298047/143‑1 (MQ=255) gcCCTGTGCCTTCTTGGGGGTGGCGTTATTTTTATAGTTACCGCTTGTGGGA‑GGGAGATTATGTTATCGCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGa < 1:60598/143‑1 (MQ=255) ccccGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATTCCATGCGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAg > 1:899658/1‑143 (MQ=255) cccGAGCCTTGCAGAGAGTGCGGTTATTTCTATAGTTACTGCTCGTGGGA‑GGGAGAGTATGTTATCTCCTCATCCCATGTGTCCGGGTTCGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGt < 2:394024/143‑1 (MQ=255) ccGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTAATGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACTGGAAGACGCCAACCCACACTCTGAGTc > 2:458987/1‑143 (MQ=255) ccTTGTAGAGAGTGGGGTGACTTGTATAGTTCCTGCTTGTGGGA‑GGGAGATTATGTTACCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCgg < 2:427411/143‑1 (MQ=255) ccTTGCAGAGAGTGGGGTTACTTTTATAGTGACTGCGTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCgg < 2:591968/143‑1 (MQ=255) ttGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGca > 2:439870/1‑143 (MQ=255) tAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCCTCTTCCTGAGTCTCCGGCacac > 1:513725/1‑143 (MQ=255) tAGAGAGTGGGGTTATTTTTATAGTTAATGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTGTCTGAGTCTGCGGCacac > 2:449167/1‑143 (MQ=255) gagTGGGGTTAGTCTTATAGTTAATGCTTGTGGGA‑GGGACATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCt < 1:773376/143‑1 (MQ=255) tGGGGTTATTTTTATCGCGACTGCTTGTGGGA‑GGGACATTGTGTTATCTCCTCAGCCCATGTGACCGGGTGAGCCGGCCAGCATCACTTGCGCTTGGCCTGGCGTTGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGAt < 1:77715/143‑1 (MQ=255) tGGGGTTATTTTTATAGTTGATGCTTGTGGGA‑GGGAGATTAGGGTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGAt < 1:617161/143‑1 (MQ=255) tGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGACTCACTTCTTCTTTACCGGACCACGCCAGCCTTCTTTCTGAGTCTGCGGCACACTGCtaac > 1:17665/1‑140 (MQ=255) ggggTTATTTTTATTGTTATTGCTTGTGGGA‑GGGACATTATGTTATCTCCTCATCCCATGTGACCGGGTTATCCGGCCATAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATa < 1:334918/143‑1 (MQ=255) gTTATTTTTATAGTTACTGCTTGTGGTACTGGGGATTCTTTTTTGT‑ATCATTCCATGTGACCGTGTGAGACGTCCAGTATTACGTTTTTCTTACCGGTCGACGCTCCGCTGCTTTCTGAGTCTGCGCCCCACTCCTGACAgac > 1:1039802/1‑141 (MQ=255) ataGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGCCACACGGCTGACAGCTAATGCAttt > 1:670245/1‑143 (MQ=255) ttACTGCTTGTGGGA‑GGGAGATTATGTTATCTCACGAAGCCAAGGGTGCTTGTTAGCCGGCCAGAATCACTTTTTCGTTACCGGACGACGTCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGc < 1:491423/143‑1 (MQ=255) aCTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGAAGCCAGCCTTCTTTCTGCGTCTGCGGCACCAGGCTGATAGCTAAGGCCTTGTCGCCg > 1:369479/1‑143 (MQ=255) | TCTGCTACATCACGTTGCGCGATCGCGCCAACAATTCCACACATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGA‑GGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCG > NC_000913/3913624‑3913907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |