Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA97.4% 229.1 / NA 78P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (37/39);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184402‑4184680
                                                                                                                                            |                                                                                                                                          
gccggCGGTTAGATGCCGGAGCCAGCGTGGTGACAAGATGGTGGGTCGTCTGCGTGACAAGGGTGTAATTGCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                          <  1:538300/140‑1 (MQ=255)
   ggCGGTTAAACGCCGGATCCAGCCTGGTGACAGGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                       <  1:701308/143‑1 (MQ=255)
         tAAACGCCGTATCCAGCCTTGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                 <  2:228290/143‑1 (MQ=255)
         tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                 <  2:228148/143‑1 (MQ=255)
           aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg                                                                                                                               >  1:970188/1‑143 (MQ=255)
            ccGCCGTGTCGAGGCTGGGGACAAGATGGCAGGGCGGCACGGTAACAAGGGTGTAATTTCTGAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                              <  1:682233/142‑1 (MQ=255)
            aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACGCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                              >  2:27151/1‑143 (MQ=255)
            aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                              >  2:670594/1‑143 (MQ=255)
                cggaTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                          <  1:317171/140‑1 (MQ=255)
                  tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATAAACCTGATCGAAGATATGCCTTACGCTGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGGGTACCGTCTAGtg                                                                                                                        >  2:268139/1‑142 (MQ=255)
                      cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                    >  2:126804/1‑143 (MQ=255)
                        gCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAACCAGCTGGGCGTACCGTGTCGTATGACCa                                                                                                                  >  1:753690/1‑143 (MQ=255)
                           tGGTGACGAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTGGCGACGCAACCGTGCTTGCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  1:496146/143‑1 (MQ=255)
                           tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGACTTACGATGAAAACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  1:997954/143‑1 (MQ=255)
                           gggTGAGAAGCTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAGGACATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCACGTATGAACATCg                                                                                                               <  1:288049/142‑1 (MQ=255)
                           gggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  2:928502/142‑1 (MQ=255)
                                  aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATAACTAAGATCAACCCGATCGAAGATATGCCTAACGATGAAAACGGTACGCCGGTAGACATCGTACTGTACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTTAgct                                                                                                        >  2:730316/1‑141 (MQ=255)
                                     aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                     >  1:406204/1‑143 (MQ=255)
                                      tgtcAGGTCGGCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCTAAGATTTGTCTTATGATGAAAACGGTACGGCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    <  2:122428/140‑1 (MQ=255)
                                      tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    <  2:1031825/143‑1 (MQ=255)
                                       ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGCACAGATCCCCg                                                                                                   >  1:382830/1‑143 (MQ=255)
                                       ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGAACCTCg                                                                                                   >  1:273077/1‑143 (MQ=255)
                                        gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGGTCCTCGa                                                                                                  >  2:999442/1‑143 (MQ=255)
                                        gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  >  2:737008/1‑143 (MQ=255)
                                          cagtcgtcACTGCATCAAGTGGTTAATTTCTAAGATCGCGCCGATCGCACAGATGCGTTACGCTGGCAACGGTACGCCGGTAGACACCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:883415/141‑1 (MQ=255)
                                             tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTAGTATGCACATCGGTCAgggacgagacaccc                                                                                             >  1:827694/1‑130 (MQ=255)
                                               gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                           <  2:1081029/143‑1 (MQ=255)
                                                 cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGACCCTCGAAACCCACCt                                                                                         <  2:523885/143‑1 (MQ=255)
                                                   cgctAACAAGGGTGTAAGTTCTACGACCAACTCCATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:312061/140‑1 (MQ=255)
                                                   cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:996487/143‑1 (MQ=255)
                                                    ggaaaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg                                                                                      <  1:418796/140‑1 (MQ=255)
                                                    ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAACCTCGAAACCCACCTggt                                                                                      >  2:131534/1‑142 (MQ=255)
                                                         cAAGCGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                                 <  2:648035/143‑1 (MQ=255)
                                                         cAACGGTGTAATTCCCAACAGCACCCCTATCGAACATATGCCGTACGATGAAAGCGGAACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                                 <  2:265610/143‑1 (MQ=255)
                                                           aGGGTGTAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCt                                                                               <  1:312150/143‑1 (MQ=255)
                                                                 tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGCATGGCTGCGaaa                                                                         >  1:1053968/1‑143 (MQ=255)
                                                                 tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         >  1:465813/1‑143 (MQ=255)
                                                                 caaTTTCTAAGACCAACCCGATCGAAGATATGCCTTGCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:389043/142‑1 (MQ=255)
                                                                  aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                        >  2:1051159/1‑143 (MQ=255)
                                                                        tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAACGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCATCGAAACCCACCTGGGGATGGCGGCGAAAGGTATCg                                                                  >  1:892987/1‑143 (MQ=255)
                                                                        tAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTACGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTATCg                                                                  >  1:746973/1‑143 (MQ=255)
                                                                               aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTTCGAAAGGTATCGGCGatga                                                           >  2:13386/1‑140 (MQ=255)
                                                                                 cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                         >  2:281812/1‑143 (MQ=255)
                                                                                    gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                      >  2:578692/1‑143 (MQ=255)
                                                                                      tCGAAGATATGCCGTACGACGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  2:374943/143‑1 (MQ=255)
                                                                                        gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGGATGGCTGCGCAAGGTATCGGCGGCAGGATGAgcgc                                                  >  2:646031/1‑143 (MQ=255)
                                                                                             tatGCCTTACGATGAAAACGTTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                             <  2:932976/143‑1 (MQ=255)
                                                                                             tatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGacccacccaatga                                             >  1:224381/1‑131 (MQ=255)
                                                                                             tatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                             >  1:228281/1‑143 (MQ=255)
                                                                                              atGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                            <  2:1088205/143‑1 (MQ=255)
                                                                                                  cggaCGGTGAAGCCGGCACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                        <  2:229469/140‑1 (MQ=255)
                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                        >  1:125243/1‑143 (MQ=255)
                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                        >  2:622819/1‑143 (MQ=255)
                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                        >  2:252311/1‑143 (MQ=255)
                                                                                                     aCGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                     <  2:541740/143‑1 (MQ=255)
                                                                                                       gaggAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  2:369225/140‑1 (MQ=255)
                                                                                                       gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGCTAGCGGCGACAAGATCAACGCCCTGCTGAAAcacca                                   >  2:587744/1‑143 (MQ=255)
                                                                                                       gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  1:848722/143‑1 (MQ=255)
                                                                                                        aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                  >  1:592912/1‑143 (MQ=255)
                                                                                                          gAACACCGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                                <  2:502511/143‑1 (MQ=255)
                                                                                                           aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                               <  2:580854/143‑1 (MQ=255)
                                                                                                            aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTgctgtttcagcatg                              <  2:57067/143‑14 (MQ=255)
                                                                                                              aCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTAGGAATCGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                            <  2:942903/143‑1 (MQ=255)
                                                                                                                     gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACGCACCTGGGTATGGCTGGGAAAGGCATCGGCGACAAGATCAACGCCAGGCTGGAACAGCAGCGAGAGGTCgaga                     >  1:1062685/1‑143 (MQ=255)
                                                                                                                     gCCGGTAGACATCGTACGGAACCAGATCGCCGGTCCACCCCGTATGAACATCGGTCAGATCCTCGAACCCCACCTGGGTATGGCTCCGAAAGGTATCGGCGACGCGACCAACGCCATGCCGAAACAGCAGCCAGACGTcgcgc                     >  1:800226/1‑142 (MQ=255)
                                                                                                                       cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATTGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGCAGTCGCGaaa                   >  1:1087995/1‑143 (MQ=255)
                                                                                                                        ggTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                  <  2:970188/143‑1 (MQ=255)
                                                                                                                            gACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg              <  2:603062/143‑1 (MQ=255)
                                                                                                                             aggtCGTACTGCAACCGTTGGGCGGACCGTCTCGGATGAACACGGGTCAGCGCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             <  2:877/140‑1 (MQ=255)
                                                                                                                             aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTCTCGGCGACACGGTCTATGCCACCCCCAAACAGCAGCAAGAAGTCGCGAAACTgcgc             >  1:786901/1‑143 (MQ=255)
                                                                                                                             aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGACACAGCAGCAAGAAGTCGCACAACTacgc             >  1:352114/1‑143 (MQ=255)
                                                                                                                             aCATCGTACTGAACAAGCTGAGCGTACCGGATCGGATGAACAGCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             <  2:159361/143‑1 (MQ=255)
                                                                                                                             aCAGCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAGCGTCGTACAGAGCCTCGAAACACACCGGGGTATGGCGGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             <  2:303597/143‑1 (MQ=255)
                                                                                                                                   tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGGAAGAAGTGGCGAAACTGcgcggagtc       >  2:178080/1‑138 (MQ=255)
                                                                                                                                     cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt     >  2:745941/1‑143 (MQ=255)
                                                                                                                                      gtaaCCCGCCGGGCGTACCGTCTTGTATGAACATCGGTCAGAGGCTCGAAACCCACCCGGGTATGGCTGCGAAAAGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc    <  1:997786/141‑1 (MQ=255)
                                                                                                                                       gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc   <  1:578692/143‑1 (MQ=255)
                                                                                                                                        aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa  <  1:252311/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184402‑4184680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: