Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,020,961 A→G intergenic (‑42/‑177) sulA ← / → sxy SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,020,9610AG98.4% 192.1 / NA 61intergenic (‑42/‑177)sulA/sxySOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (33/27);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020822‑1021089
                                                                                                                                           |                                                                                                                                
ataggcgccgCTGTGGTGGTTTCCGGAGTGACACGCGCAATTTGACTTGCGGCGGATGAGCACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCAt                                                                                                                               <  1:294165/134‑1 (MQ=255)
   aGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCa                                                                                                                            >  1:487003/1‑143 (MQ=255)
     cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCCATTTTACTTGCTGCGGATGAGAACGACGCAGAACGAGGTGCATCGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGGGTTACCATCTGAATGTACGGTGCACCCAgc                                                                                                                          >  1:325682/1‑142 (MQ=255)
      ccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCGGTg                                                                                                                         >  1:936566/1‑143 (MQ=255)
      ccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTg                                                                                                                         >  2:183463/1‑143 (MQ=255)
       cGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGa                                                                                                                        <  1:758600/143‑1 (MQ=255)
       cGGCTGTAGGGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGTAAGGAGTTCAAACCCTGATGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGa                                                                                                                        <  2:258242/143‑1 (MQ=255)
          cTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGacaa                                                                                                                     >  1:1112360/1‑143 (MQ=255)
              ttggtTTTCCTTAGAGACACGCGCAATTTTACTTGCGGCGTATGATAACGACGAAGAACGATGTGCAGAGCCTGAAGTATACATAAGCAATCCAGCCCCTGTGAGTTATTGTATGGATGTACAGTGCATCCAGAGACAACaaa                                                                                                                 <  1:461383/140‑1 (MQ=255)
                 gtTTGCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGAt                                                                                                              <  2:279803/143‑1 (MQ=255)
                  ttgtCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGCACGACGAAGAACGATGTGCATAGCCTGACGTGTACATAATCAATCCAGCCCCTGTGACGTACTGTGTGGCTGTACAGTGCACCCCGTTGCAACAAAGATc                                                                                                             >  1:750597/4‑143 (MQ=255)
                    ttCCGTAGAGACACGCGCAATCTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCaa                                                                                                           <  2:692909/143‑1 (MQ=255)
                     tCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAAc                                                                                                          <  2:373854/143‑1 (MQ=255)
                     tCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGCTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAAc                                                                                                          >  1:890849/1‑143 (MQ=255)
                      ccGTAGAGACACGCGCAACTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATCGATGTACAGTGCATCCAGTGACAACAAAGATCAAcc                                                                                                         <  2:721246/143‑1 (MQ=255)
                        gTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGCGTTACTGTATGGATGTACAGTGCAACCAGTGACAACAAAGATCAACCCt                                                                                                       >  1:53563/1‑143 (MQ=255)
                        gTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACACCAAAGATCAACCCt                                                                                                       >  2:440058/1‑143 (MQ=255)
                        gTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGACTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGCTGTACAGTGCATCACGTGACAACACAGATCAACCCt                                                                                                       >  1:941737/1‑143 (MQ=255)
                              acacGCGCAAGTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTc                                                                                                 <  2:519488/143‑1 (MQ=255)
                               cacGCGCCATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCg                                                                                                <  1:619884/143‑1 (MQ=255)
                                     tagttttACTTGCTGCGGATGGGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCGTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAaga                                                                                          <  1:469951/140‑1 (MQ=255)
                                     gaaTTTTACTTGTGGCGGCTGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCACTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAaga                                                                                          <  1:260083/142‑1 (MQ=255)
                                     cacgtgtccatgcggcGGATGAGAACGAGGAAGCGACACGTGGTGGGCGTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAaga                                                                                          <  2:810545/133‑1 (MQ=255)
                                      aaTTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCGTGCAGGGTACATAGTCAATACAGACCCTGTGAGTTACTGTATGGATGTACAATACATCCAGTGAACACAACTATCAACCCTCTTCTCGCAAcgag                                                                                         >  1:1050961/1‑143 (MQ=255)
                                         tttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCt                                                                                      <  1:628578/143‑1 (MQ=255)
                                              ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACGACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaa                                                                                 >  1:768944/1‑143 (MQ=255)
                                              ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGCGCATCCAGTGCCAACAAAGATCAACCCTATTTTCGGACAGAGCCTCGCaa                                                                                 >  1:773252/1‑143 (MQ=255)
                                              gtgatgcGGATGAGAACGACGAAAAACAATGGGCATAGCCTGAAGTGTACATAATCAATACAGCTCCTGTGAGTTACTGTATGGATGAACAGAGCATCCAGAGAGAAAAAAGATCAACACTATTTTCGGAAAGAGCCTCGCaa                                                                                 >  1:614068/5‑143 (MQ=255)
                                                  tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAttt                                                                             >  2:1083065/1‑143 (MQ=255)
                                                   gcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                            >  2:706321/1‑143 (MQ=255)
                                                      gATGAGAACGACGAAGGACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTc                                                                         <  1:43301/143‑1 (MQ=255)
                                                          agaACGACGAAGAACGATGTGCATAGCCTGAAGGGTACATAATCAAGCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                     <  2:11718/143‑1 (MQ=255)
                                                              cgacgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTCAGGAAAGAGCCTCGCCACTACTGGCCTTGGTGa                                                                 >  1:782651/1‑143 (MQ=255)
                                                                acgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACg                                                               >  2:985557/1‑143 (MQ=255)
                                                                acgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACg                                                               >  2:771159/1‑143 (MQ=255)
                                                                     gaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGaaa                                                          >  1:26129/1‑143 (MQ=255)
                                                                     gaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAATTTGTCATTGGGGACGGGaaa                                                          >  1:874032/1‑143 (MQ=255)
                                                                       ccGATGTGCATAGCCTGAAGTGTACATAAACAATCCCGCCCGTTTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAAc                                                        <  2:1056232/142‑1 (MQ=255)
                                                                         gATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACAt                                                      <  2:1016211/143‑1 (MQ=255)
                                                                         gATGCGCATAGCCTGAAGTGTACAGAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACGACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACAt                                                      <  2:382736/143‑1 (MQ=255)
                                                                            gtgCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTCTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATaaa                                                   <  2:601644/143‑1 (MQ=255)
                                                                              gCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAAtt                                                 >  1:1874/1‑143 (MQ=255)
                                                                              gCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGAAGGGGACGGGGAAACGTAACtt                                                 >  2:148317/1‑143 (MQ=255)
                                                                               cATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTa                                                >  2:161828/1‑143 (MQ=255)
                                                                                  aGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGGTACTGTATGGATGGACAGTGCATCCAGTGACTACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATc                                             <  2:317450/143‑1 (MQ=255)
                                                                                  aGCCTGAACTGTATATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATc                                             <  2:970353/143‑1 (MQ=255)
                                                                                    ccTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATACATTAATCtt                                           >  1:517991/1‑143 (MQ=255)
                                                                                               cATAATCAATCCAGCCCCTGTGAGTTACTGTCTGGATGTACAGTGCACCCCCTCACAACAAAGATCAAACCTATCTTCTGAAAGAGCCTCGCAAATTTTGTCGTTGCTGACGCGAAAACATAAATTAATCTTGCCCCTTAAGa                                >  1:909559/1‑143 (MQ=255)
                                                                                                 tAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTCAGact                              >  1:856728/1‑141 (MQ=255)
                                                                                                   atcactcCAGTCCCTGCGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATaa                            <  2:41122/143‑1 (MQ=255)
                                                                                                   atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAAAAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCCAATTTTGTCGTTGTTGACGGGCACACATAAATTCATCTTGCCCCTTCAGAATag                            >  2:911745/1‑142 (MQ=255)
                                                                                                      aatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGtt                         <  2:174745/143‑1 (MQ=255)
                                                                                                        tcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGCATACATAATTTAATCTTGCCCCTTAAGAATAAGTTGc                       >  2:203964/1‑143 (MQ=255)
                                                                                                          cAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCt                     >  2:321029/1‑143 (MQ=255)
                                                                                                             ccccTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATCAATTAATCTTGCCCCTTAAGAATAACTTGACTAtt                  >  1:40558/1‑143 (MQ=255)
                                                                                                                 ggcgAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCg              <  2:1026237/140‑1 (MQ=255)
                                                                                                                         aCTGTATGGATGTACAGTGCCCCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAAc      <  2:865556/143‑1 (MQ=255)
                                                                                                                         aCTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAAc      >  2:150742/1‑143 (MQ=255)
                                                                                                                          cTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACg     >  1:705041/1‑143 (MQ=255)
                                                                                                                           tGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTGACgg    >  2:972525/1‑143 (MQ=255)
                                                                                                                             tagggATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTGGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAt  <  1:604076/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                
ATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020822‑1021089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: