Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	111.0 / NA	40	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/17);  total (23/17)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925                                                                                                                                             |                                                                                                                                           ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTc                                                                                                                                          >  2:579009/1‑143 (MQ=255) ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGACTGATATGCAGGCTc                                                                                                                                          >  1:466960/1‑143 (MQ=255)   aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCACTTCACCGTGATTCATATGCAGGCTCTg                                                                                                                                        >  1:107721/1‑143 (MQ=255)           gagTGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAAGTTGTCTTTGTGGTTGCGGTAGAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct                                                                                                                                <  1:127637/143‑1 (MQ=255)                 taaCAGCTGGTATTTGTTGCTGGCGGGGTTGGATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGGATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                          <  1:744042/142‑1 (MQ=255)                   aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt                                                                                                                        >  2:748160/1‑143 (MQ=255)                        tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   <  2:864300/143‑1 (MQ=255)                         gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGTCTAAGCGTCGCCTGTTCTACttgacac                                                                                                                  >  2:718673/1‑138 (MQ=255)                             ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTCCCACTTTCCACGGtt                                                                                                              >  1:597520/1‑143 (MQ=255)                                    cTGGCGCGGACGAATTTGCCTTTGGGCTTGCGGTAAACGTGGTTGTACCTGGAGAATTTTTTCGGTGACGAACTTACCTCCATTTTTCCGTGATTGATATTCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGTCGTa                                                                                                       <  1:446381/143‑1 (MQ=255)                                     tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCCGGCCCGGCGCCGCCTCTTCTACTTTCCACGGTTCCCCGtcc                                                                                                      >  2:37769/1‑141 (MQ=255)                                              cGAGTTTGTCTTTGTGCTTGGGGTGAAAGTGGAAGTAGCCGGCGAATTCCTTCCGTGACGAGGTGATCGGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc                                                                                             <  1:230818/143‑1 (MQ=255)                                               gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGTCGTACTccaccacca                                                                                            >  2:460847/1‑143 (MQ=255)                                                  tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCAtgc                                                                                         >  2:1091/1‑141 (MQ=255)                                                     gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                      >  2:1097874/1‑143 (MQ=255)                                                     gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTCTCCACGGTTTGACGTAGTccacgaccgccggcc                                                                                      >  1:3271/1‑136 (MQ=255)                                                       cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACCCCACCACCATCCGCCgg                                                                                    >  1:317558/1‑143 (MQ=255)                                                       cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCCGCGTCGCCTCTTCTACTTTTCACGGTTCTCCGTACTTCTCTAGCATCCGCAgg                                                                                    >  2:973056/1‑143 (MQ=255)                                                                tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACGGTTCGCCAGACTCGACCACCAGACGCCGGAAGATCTCg                                                                           >  1:325305/1‑143 (MQ=255)                                                                   tcggacagcggACGTCCCCGGACAATTTTTGCTGTGACGACGTGATCTTCATTTCACCGTGATGGATATGCAGGCTTTGCCTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                        <  1:280262/134‑1 (MQ=255)                                                                     tagatgtggccgTACCCGGAGAATGTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGTTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                      <  1:357638/132‑1 (MQ=255)                                                                         aGTCGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                  <  2:391979/143‑1 (MQ=255)                                                                                   cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                        <  2:994350/143‑1 (MQ=255)                                                                                    ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGCAGATGTCGTAATCCTGTGCCGCTTTCAg                                                       >  1:277741/1‑143 (MQ=255)                                                                                        agaATTTTTTCGGTTACGACGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                   <  2:439503/143‑1 (MQ=255)                                                                                          gattgcttCGGTGACGCGGTGATCTGCATTTCACCGTTCTTGATATGCAGCCTCTGCGTCGCCTCTTCTACTTCCCACGGTTCGCCGTACTCCACCACCATCCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt                                                 <  1:717556/137‑1 (MQ=255)                                                                                            ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCCGATGTCGTAATGCTGTGCCGCTTTCAGTTCGGTac                                               >  1:900849/1‑142 (MQ=255)                                                                                             tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTGCAGTTCGGTATc                                              >  2:40184/1‑143 (MQ=255)                                                                                              tttacgggctcGATGTGATCTGCATTTCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACCGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                             <  1:959400/133‑1 (MQ=255)                                                                                                   ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCTACTTTCCACGGTTCCACATACTCCACCACCATCCCCAGGAAGCTGTCGTAACCCTGTGCCGCCTTCAGTACGTCAGCGaacac                                        >  1:128910/1‑142 (MQ=255)                                                                                                    gttaCGCGGTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCATCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                       <  2:818556/140‑1 (MQ=255)                                                                                                    gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGGCGTACTCCACCACCAACCGCAGGAAGATGAGGTAATCCTTTGCATATTGCAATTCGGTGTAGaacaaa                                       >  1:44882/1‑143 (MQ=255)                                                                                                         gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGACGGCATTGTTTGCCGCTTTCAGTTCGATAGCGaacaaacaacc                                  >  1:600236/1‑140 (MQ=255)                                                                                                           ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCACTTTCAGTTCGGTATCGCACAAACACTCtt                                >  2:599322/1‑143 (MQ=255)                                                                                                              gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                             <  2:912401/143‑1 (MQ=255)                                                                                                                          aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCAACCGCAGGCACCCGTCGTAATCCTGTGCCGCTCTCACTCCGGTGTCGGACCCCCCCTCTTTGACGCGCCATcccc                 >  1:445519/1‑143 (MQ=255)                                                                                                                            cGTGATTGCTACGCAGGCGCTGCGGCGCCTCTTCTACTTTCCACGGTTGGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg               <  1:214572/143‑1 (MQ=255)                                                                                                                            cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCTCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCCTTGAAACGCCCTGCCCAg               >  2:195881/1‑143 (MQ=255)                                                                                                                                    ctatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc       <  2:385094/142‑1 (MQ=255)                                                                                                                                         cAGGCTCTGCGTCGCCTCCTCGACTTGCCACGGTTCGCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt  <  1:763177/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑