| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,415,805 | A→C | D354A (GAT→GCT) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,415,805 | 0 | A | C | 98.2% | 165.0 / NA | 57 | D354A (GAT→GCT) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (33/22); total (34/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAC > NC_000913/2415665‑2415939 | cGGTTACGAAATGGACGCGCGCATTTCTAAACTATGAGAACGCACTGTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTGCGGTTGACGCTc > 2:767276/1‑143 (MQ=255) ggTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCa > 2:578783/1‑143 (MQ=255) gTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCAc > 1:825005/1‑143 (MQ=255) tACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCCACCTGGAAGTTCCGGTTGACGCTCACgc > 2:335134/1‑142 (MQ=255) tGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATc > 2:794360/1‑143 (MQ=255) aCGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGCTCCGGTTGACGCTCACGAACGTATCcag > 1:617559/1‑143 (MQ=255) gcgcgcATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACAAACAACTGGCAGACCTCTCTGAGCCTGCAGAGCGTCAACATGGAAGTTGCGGTTGACGATCGCGAACGGATcacggc > 1:894820/1‑138 (MQ=255) cgcgcgTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTAAGTATTGTGTACTCCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa < 1:127586/143‑1 (MQ=255) cgcgcATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa < 2:328680/143‑1 (MQ=255) cgcgcATTGCTAAACTGTGCGAACGTGCTTTCGCTGCCGGCCTGCCGGTATTTATGGTGAACACCAACACCGGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGaaa < 2:994671/143‑1 (MQ=255) ctttCTAAAGTGTGCGCACGTGCTTTCGCTACCGGACTGCCGGTCTTTGTGGTGAACCCCACCACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTACACCAGTGCCAAGCAATTGACGCTCACGAACGTATCGAGAAAGTTCa < 1:676013/142‑1 (MQ=255) aTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGACCGTATCGAGAAAGTTCa > 2:1015224/1‑143 (MQ=255) aTTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCa < 2:574431/143‑1 (MQ=255) ttacTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAg < 2:294698/140‑1 (MQ=255) ttCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCTGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGCTCAgg > 1:820526/1‑143 (MQ=255) tAAACTGTGCGAACGTGCTTTCGCTACCGACCTGCCGGTATGTGTTGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAAt < 2:1056236/143‑1 (MQ=255) tgCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCCACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGACCGTTCAGGAATACggcgc > 1:406949/1‑139 (MQ=255) gCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGACCGTATCGAGAAAGTGCAGGAATACGTTGCt > 2:1015683/1‑143 (MQ=255) gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGACGCTCCTGTTGACGCTCACGACCGTATCGAGAAAGTTCAGGAATACGTTGATaa > 1:145634/1‑143 (MQ=255) gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa > 2:786796/1‑143 (MQ=255) gAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTACCGGTTGACGCTCACGAACGTATCGAGAAAGTTGAGGAATACGGTGCTaa > 1:557654/1‑143 (MQ=255) gCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCACTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCACACGAACGTATCGAGAAAGATCAGGAATACGCTACTAACTACAACAACGc > 2:11351/1‑143 (MQ=255) tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTg > 2:578560/1‑143 (MQ=255) cTGCCGGTGGTTGTGGTGACCACGCCCACCTGGCAGACCTCTCTGAGCCGGTAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAt < 1:705394/143‑1 (MQ=255) tGCCTGTATTTATGGTGACCACCAACACCTGGCAGACCTCTGTGAGCCTGCAGAGCTTCAACCTGGAATTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATc < 1:86635/143‑1 (MQ=255) ccGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGa > 2:888151/1‑143 (MQ=255) cGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGAGTGGATCgga > 2:237434/1‑141 (MQ=255) gTATTTATGGTGACCACCAACACCTGCCAGCCCTCGCTTAGCCTGCATGGCTTCAACCTTGAAGTTCCCGTTGACGCTCACTAACGTATCGAGATAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTCGATCGAAtc < 1:875897/143‑1 (MQ=255) tttATGGTGACCACCAACACCTGGCAGATCTCTCTGAGCCTGCAGAGCTTCAACCTGGCAGCTCCGGTTGACGTTCACGTACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATctct < 2:238853/143‑1 (MQ=255) gacggTGAACGCCAACGCCTGGCAGACCTCGCTCAGCCTGCAGAGCTTCAACCTGGAAGGTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGa < 2:62783/140‑1 (MQ=255) aTGGTGAACACCAACCCCTGGCAGCCCTCTCTGGGCCTGCAGAGCGTCGACCGGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAc < 1:969346/143‑1 (MQ=255) cacaccaacgccGGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTTCATCAACGCTGACTGGATCGAATCTCTGACTGCCAc < 2:722369/142‑1 (MQ=255) aacaccaacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTACCTACCTCCACGCTGCCTGGATCGAATCGCTGACTGCCAc > 1:346485/1‑143 (MQ=255) caccaacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGCAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACACCAACGCTGACTGGATCGAAGCTCTGACTGCCACtt > 1:955069/1‑143 (MQ=255) ccctgacgcggtagagCTCGCTGAGCCTGCAGAGCGTCAACCTGGTAGCTCCGGGTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGCCTGGATCGAATCTCTGACTGCCACTTCt < 1:798161/131‑1 (MQ=255) caacaccTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTg > 2:730710/1‑143 (MQ=255) cTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCa > 2:584068/1‑143 (MQ=255) tGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGGTCCGGTTGACACTCACGAACGTATCGAGCAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGAGCAg > 2:736095/1‑143 (MQ=255) aCCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcg > 2:188455/1‑143 (MQ=255) ctctctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGAGTGCCACTTCTGGGCGCCGCCgccgtc > 2:863269/1‑143 (MQ=255) ctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtc > 2:296122/1‑143 (MQ=255) ctGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGACACTTCTGAGCGCAGCCGTCgtctgtc > 2:296458/1‑143 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgt > 2:41823/1‑127 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgt < 1:41823/127‑1 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg > 2:708411/1‑143 (MQ=255) gCAGAGCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg > 2:847796/1‑143 (MQ=255) agagCTTCAACCTGGAAGTTCCGGTTGACGCTCACGAGCGTAGCGAGAAAGTTCAGGAATACGTTGCTCACCACGTCAACGCTGACTGGATCGCAGCTCTGACTGCCACTGCGGAGCGCGGCCGTCGCCCGTCCCCGCCcgcg > 1:909513/1‑143 (MQ=255) cTTTAACCTGGAAGTTCCGGTTGACGCACACGACCGTATCGAGAAAGGTCAGGAAGACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc < 2:1036912/143‑1 (MQ=255) cTTCAACCTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACCTCAACGCTGACTGGATCGAATCTCTGCCTGCCCCTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc > 2:662869/1‑143 (MQ=255) aaCCTGGAAGTTCCGGTTGACGCTCACGCACGTATCGAGACAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTa < 2:474943/143‑1 (MQ=255) ctgcaagcgccGGTTGACGCTCACGCACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGTCTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCa < 2:160351/134‑1 (MQ=255) cTGGAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCa > 1:816573/1‑143 (MQ=255) gAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTCTCAGct > 2:1050975/1‑143 (MQ=255) gAAGTTCCGGTTGACGCTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGct < 2:956485/143‑1 (MQ=255) gtgccGGTTGACGCGCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACGGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGctgac < 2:145634/140‑1 (MQ=255) cGGTTGACGCTCACGACCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGAGCGAATCTCTGCCGGCCACCTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGaa < 2:67936/143‑1 (MQ=255) ggTGGACGCTCACGAACGTACCGAGAAAGTTCAGGAATGCGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTCACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAc < 2:581488/143‑1 (MQ=255) | CGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAACGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAAC > NC_000913/2415665‑2415939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |