| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,315,392 | 0 | G | T | 29.8% | 70.5 / 28.1 | 58 | H50N (CAT→AAT) | phnO | aminoalkylphosphonate N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (25/15); new base T (12/5); total (38/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAG > NC_000913/4315251‑4315532 | gccgcgtGCCGACGTTCGGACCGCGGTCTTGCGGGCTTGGCACCGTGTCCGGGGTGGCGCCGATCCGGTTGACATGATGCAGATGAACCTGAAATTGCAGGAACATTATCGGCACTATGTCGCCATCAAGCAGCGCCAGATgg < 1:749290/137‑1 (MQ=255) ttACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATCTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTa < 2:115120/143‑1 (MQ=255) tACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAg < 2:759711/143‑1 (MQ=255) ccGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCAt > 2:532708/1‑143 (MQ=255) aGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGCTGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTc > 2:56973/1‑143 (MQ=255) aGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCCGCGCCAGATGGTAGCGCATGTTTgggcc > 2:349694/1‑141 (MQ=255) ccTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCACATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTACCGCATGTTTGGGTCGCGCCGCTTg > 1:648973/1‑143 (MQ=255) cTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCCGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTgg > 1:201525/1‑143 (MQ=255) cTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGACAGTTGGGCATGGTTGGGTGGCGAAgcatgg > 1:419568/1‑138 (MQ=255) tGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGc > 1:886107/1‑143 (MQ=255) ttACCACCCACTCCTGAAGTGCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTaaaa < 1:626832/143‑1 (MQ=255) accaccAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGAGGAAACTGCAAATGCAGGCCGATCATGCCGACAACATCGCCATAAAGCAGCGCCAGATGGAAGCGCAAGTAAGGGACACACAGAGAGGCGATACAAcc > 1:744267/1‑143 (MQ=255) cctcgatgtgggcGATCGAGTGGACATGGTGCAGAGGAAACGGCAAATGCAGGCCGATCAGGCCGACAACTTCGCCATCAAGCCGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAc < 2:284816/131‑1 (MQ=255) cTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAACTGCAGGCCGATCATGCCGACAGCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAcg > 1:459802/1‑143 (MQ=255) cTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAcg > 1:794563/1‑143 (MQ=255) tttCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGACGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtgg < 2:622273/143‑1 (MQ=255) ttCGCCGACCCCGTCGACATGATGCAGATGAAACTGCAAATGCAGGCCGCTCATGCCGCCAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtggt < 1:585171/143‑1 (MQ=255) gCCGATCCAGTTGACATGATGCAGATGAAACTGCAACAGAAAGCCGATCATGACGACAACTTCGCCATCAAGCAGCGCCAGAAGGTAGCGCATGTTTGGGTCGCGCAGACTGGCGTGAAAACCCACGCGAAAGGCGTggtggt > 1:745673/1‑143 (MQ=255) gCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCGTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTggtggt > 2:746121/1‑143 (MQ=255) gCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTggtggt > 2:24075/1‑143 (MQ=255) cAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGGCCAACTc > 1:236406/1‑143 (MQ=255) aGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGAAAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTcc < 2:440841/143‑1 (MQ=255) gtggACGTGATGCAGCTGAAACTGCAAATGCAGGGCGATCACGCCGACGACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTGTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCg < 2:147346/140‑1 (MQ=255) gACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTTGCTTTAAAACCCAATCGAAAAGAGTAGTGGTAAAACTCCGCCt > 2:521621/1‑143 (MQ=255) tgatgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGttt > 2:782821/1‑143 (MQ=255) gatgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTCGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGtttt > 2:187773/1‑143 (MQ=255) gtgCAGATGAAACTGCAAATGCGGGCCGATCATGCCGACAACTTCGCCCTCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTa < 1:575886/142‑1 (MQ=255) cAGATGAGACTGCAAAGGCGGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt < 1:271692/143‑1 (MQ=255) cAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAGGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt < 2:31518/143‑1 (MQ=255) aGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTc < 2:795220/143‑1 (MQ=255) aaCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAAt > 2:418892/1‑143 (MQ=255) aCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGATCCGCAAGGTTGGGTAGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATc > 2:623041/1‑143 (MQ=255) tGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAg > 2:482782/1‑143 (MQ=255) cAAATGCAGGCCGATCATGCCGAAAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCACGCAGATCGGCGTTAAAACCCACGCGCAACGCGTGGTGGACAAAATCAGCCTGATTGAggagccaaagaaacg > 1:84212/1‑129 (MQ=255) aaaTGCGGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAgcgc < 1:649636/143‑1 (MQ=255) ggCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAATACGCACTCGAGACGCGTGGTGGTCAACCTCCGCCTGTTTTAGCTCACCAGTCAGCGCGTAAccc > 1:225767/1‑143 (MQ=255) gCCGATCATGCCGTCAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACcg > 2:169112/1‑143 (MQ=255) gATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTGTTAGCTCACAAATGAGCGCGTAAGCagcga > 1:533394/1‑142 (MQ=255) aTCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGAGTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTACTTTAGCTCACAAATCAGCGCGTAAACagcggc > 1:535289/1‑141 (MQ=255) tCATGCCGACGAGTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCg < 1:195860/143‑1 (MQ=255) tCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGGAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCg < 2:811423/143‑1 (MQ=255) cGACAACTTCGCCATCAAGCCGCGCCAGATTGTAGCGCATGTCTGGGTCGCGCAGATTGGCGTTCAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCg < 2:745673/143‑1 (MQ=255) cGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAGACTTCGCCTATTTTAGCTCACAAATCAGCGCGTAAACCGCGTAGGTGTCg > 1:439361/1‑143 (MQ=255) cGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCg > 2:549711/1‑143 (MQ=255) aaCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGACACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGAGTCGTACt > 2:231001/1‑143 (MQ=255) ttCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAGAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTGTTAGCTCACAACTCAGCGCGTAAACCGCGTGGGGGTCGGACTggg > 1:487954/1‑141 (MQ=255) ccATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGc < 2:251891/143‑1 (MQ=255) cATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc > 2:471726/1‑143 (MQ=255) aaGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGc > 2:878632/1‑143 (MQ=255) accgcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAg < 2:441510/141‑1 (MQ=255) gcgcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGc < 1:418892/143‑1 (MQ=255) cgcCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt > 2:13056/1‑143 (MQ=255) cgcCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt > 1:860988/1‑143 (MQ=255) gacAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCAACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTCGGTCACAAATCAGCACGTAAACCGCGCAGGTGTCGTAATGCGTGGCCAGACGAAGcac > 1:486873/3‑141 (MQ=255) ccAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTcc > 2:500006/1‑142 (MQ=255) ccAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTca < 2:378708/143‑1 (MQ=255) cAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCCcac > 1:123281/1‑143 (MQ=255) gATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACaa > 1:141757/1‑143 (MQ=255) aTGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAg > 1:118289/1‑143 (MQ=255) | ACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAG > NC_000913/4315251‑4315532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |