Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	101.7 / NA	38	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/13);  total (25/13)

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132648‑2132924                                                                                                                                            |                                                                                                                                          gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAGTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCAGGctct                                                                                                                                        >  2:454431/1‑143 (MQ=255)         agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACTAGGTCATCTGCATTTCACCTTGATTGATATGCAAGCTCTGCGTCTcc                                                                                                                                >  2:651526/1‑143 (MQ=255)                aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                         >  2:470134/1‑143 (MQ=255)                  aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCCCTGCGTCGCCCCTTCTCCt                                                                                                                       >  2:837419/1‑143 (MQ=255)                    aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                     >  2:101906/1‑143 (MQ=255)                                 tggttgcgtGGTCGGATTTGCTGTTGTGCTGTCGTTGAGAGTGGAAGTGGCCGGAGGATTTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                        <  1:244597/138‑1 (MQ=255)                                  gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATCTGCAGGCTCTGCGGCGCCTCTTCTACTTTCCACGCGTCGCCgc                                                                                                       >  1:503774/1‑142 (MQ=255)                                    tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGCAc                                                                                                     >  1:481367/1‑143 (MQ=255)                                    tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGGGATCTGCATTTCACCGTGATTCATATGCAGGCGCTGCGTCGCCTCTCCTACCTTCAACGGTCCCCCGTAc                                                                                                     >  1:294799/1‑143 (MQ=255)                                    tGGCGCGGTCGAATTTGTATTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGGCCCTGCGGCGCCTCTTATCATTTCCACGCTTAGCCGtcc                                                                                                     >  1:584090/1‑141 (MQ=255)                                      gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGCCGTACTc                                                                                                   >  1:801454/1‑143 (MQ=255)                                      gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGTTCGCCGTCCTc                                                                                                   >  2:833191/1‑143 (MQ=255)                                          ggTTGAATTTGTCTTAGTGCTTGCGGTAAAAGTGGAAGGACCCGGAGAATGTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                               <  2:691644/143‑1 (MQ=255)                                            tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCAGTTCCGCATACTccaccac                                                                                             >  2:563999/1‑143 (MQ=255)                                             cGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc                                                                                            <  2:11983/143‑1 (MQ=255)                                                aTTTGGCTTTGTGCTTGCGGTAAACGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc                                                                                         <  2:115498/143‑1 (MQ=255)                                                   tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                      <  2:626307/143‑1 (MQ=255)                                                     tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAg                                                                                    >  1:242776/1‑143 (MQ=255)                                                            gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGTTCGCCGTACCCCACCACCATCCGCAGGACGATg                                                                             >  1:481810/1‑143 (MQ=255)                                                                 cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCgct                                                                        >  2:457275/1‑141 (MQ=255)                                                                      aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGCTTCGCCGTACTCCACCACCATCCGCCGGACGCTGTCCTCATCCt                                                                   >  1:270719/1‑143 (MQ=255)                                                                        aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCCCCGGACGATGTCGTAATCCtgt                                                                 >  1:607753/1‑143 (MQ=255)                                                                            gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTGGCCGTACTCAACCACCATCCGCAGGAAGAAGTAGTAGTGCTGTGCCg                                                             >  1:485071/1‑143 (MQ=255)                                                                                tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATGCGGAGGAAGATGTGGTAATCGTGTGCCGCgtt                                                         >  2:260417/1‑143 (MQ=255)                                                                                  cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCCACGGTTCGGCGTACTCCACCACCATCCGCAGGGAGATGTCGTCATAATGAGCCGCCTTCa                                                       >  1:722651/1‑143 (MQ=255)                                                                                   ccGGAGGATTTTTTCGGTGACGATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                      <  2:76062/143‑1 (MQ=255)                                                                                   ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTACACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTcgg                                                      >  2:459185/1‑141 (MQ=255)                                                                                        gcatgttttCGGTGACGGGGTGATCTGCACTTCACCGTGATTGACATGCAGCCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                 <  1:837419/138‑1 (MQ=255)                                                                                         agctttttCGGTGAGTGGGGGTTCGGCGTTGCACCGTGTTTGATCTGCAGGCTCTGCGTCGCCTCGTCGACTTTCCACGGTTCGCCGTACGCCACCACCCTCCGCAGGAAGATGTCGTAATCCGGTGCCGCTTTCAGTTCGGt                                                <  1:685141/140‑1 (MQ=255)                                                                                                     gACGAGGTGATCTGCATTTCACCGTGTTTGATATGCAGGCTCTGCGTCGCCTATACACGTATCCACAGTTCTCCGTACTCCCCCACCACCCGCAGGAAGATGTCGTAATCCTGTACCGCCTTCAGTTCGGTATCGaacacccc                                    >  1:648387/1‑139 (MQ=255)                                                                                                          ggTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCGTTCAGTTCGGTATCGAACAAACACTCtt                               <  1:606518/143‑1 (MQ=255)                                                                                                                cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc                         <  1:235933/143‑1 (MQ=255)                                                                                                                 tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGACGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCTTTCCGTGCGGTATCGAACCAACACTCTTTGCAACg                        >  2:79697/1‑143 (MQ=255)                                                                                                                  gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATACTGTCGTTCTTTCAGTTCGGTATCGAACAAACACTCTTTGACACGc                       >  2:759298/1‑143 (MQ=255)                                                                                                                     tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt                    <  2:528667/143‑1 (MQ=255)                                                                                                                     cttCACGGTGATTGGTATGCAGTCTCGGCGCCGCCTCGTCTACTTTCCACGGTCCGCGGTACTCCACCCCCTGCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGAAACGCCAt                    <  1:440018/142‑1 (MQ=255)                                                                                                                             tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGCAACGCCATGCCCCGGt            >  2:325158/1‑143 (MQ=255)                                                                                                                                      cgCAGGCTGTGCGTCGCTTCTTCTACGTTCGGCGGTTCGCCGTACTCCACCACCTTCAGTAGGAAGATTTCGTAAGCCCGTGCCGGCTTCAATTCGGTATCGAACAAACACTCTTTGAAACGCCATGGCCAGGTAAAGACCTg   <  1:866646/142‑1 (MQ=255)                                                                                                                                       gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg  >  2:555666/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                          GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132648‑2132924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑