Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,469 C→G 16.1% S3R (AGC→AGG ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4690CG16.1% 161.6 / 7.4 73S3R (AGC→AGGttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (10/45);  new base G (6/6);  total (16/57)
Fisher's exact test for biased strand distribution p-value = 2.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206234‑3206707
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   
tAAAGGCCAGGTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                                   <  2:1056758/238‑1 (MQ=255)
tAAAGGCCAGCTTTTTAAGATTGTTAAATTTGCGCAGTTGGGAAAAGCGCAATGTATTTCGCAAAACATTGCCATTCCCTGGTCTGGCGCAATATTTATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAGTCACGGGGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTTGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                                   <  1:85908/238‑1 (MQ=255)
tAAAGGCCAGCTATTCCGCATCGTTAATTATCCGCAGTTGGGATAAGCCCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                                   <  2:949949/238‑1 (MQ=255)
tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                                   <  2:989127/238‑1 (MQ=255)
tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGAAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                                   <  2:822593/238‑1 (MQ=255)
caaaGTCCGGGTATTCACCCTCGTTTATTCTCCGCAGTTGTTATTAGCGAAGTTTATTTCCAAAAACATTGCCCTTCCCTGCTTTGGCTCCAAATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTTATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                                   <  1:970451/237‑1 (MQ=255)
  aaggcccggtttttcgcatgtttAATTTTCCGCAGTTTTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                                                                                                                                                                <  1:455370/218‑1 (MQ=255)
  aaagccctttatttcagaTCGGTTATTTTCCGCAGGTGGTATAAGGGCAGTGATTTTCGCAAAACCTTTCCGTTCCCTGTTCTGGCGAAATATTAAGTCTTATTTTCGAGTTGATTCACGACCTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTAGTTGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATTCACAAACCGGGGTTGCCATGAGGATCGATAg                                                                                                                                                                                                                                                <  1:599061/222‑1 (MQ=255)
       cccttttttacgctttttAATTGTCCGCTGTTGTGATAAGCGCAGTTTTTTTCACAACACATTGCCATTCCATGCACTGGAGAAATATTCATTCTTATTTTCGCATTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATGTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                            <  1:1216454/222‑1 (MQ=255)
       cAGTTATTCTGCATGTTTAATTCTCCGCATTTGGGATAAGTCTAGTTTATTTCGCAAAACATTGCCATTCCCGGGTCTGGCGCAATATGCATTCTTATTGTCGCATTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                            <  1:39052/238‑1 (MQ=255)
       cAGTTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                            <  2:677168/238‑1 (MQ=255)
       cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                            <  2:599807/238‑1 (MQ=255)
       cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                            <  2:191506/238‑1 (MQ=255)
                 gtcttgTTAATTTGCCGCAGTTGTGCTAAGGGCAGTGTTTTTGCCAAAACATTGCCATTCCCTGCGCTGGCGCAATATTCATTCTTATTTTCGCGGTGATTCACGAACTGGTAAGTACATATTGACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                               <  1:416545/234‑1 (MQ=255)
                 gctttttttattttcccggtttGGGAAAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                               <  1:48755/219‑1 (MQ=255)
                  cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCATCCAAAATAAAAAATTGAGTTGTCATGATTAGGGGAAGAAAAAAACAAAAgaac                                                                                                                                                                                                                               >  2:291498/1‑236 (MQ=255)
                        tAATTTTCCGCCGTTGTGATAAGCGCCTTTTATTTCTCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                                                                                                                                                       <  1:1247563/239‑1 (MQ=255)
                        tAATTATCCCCAGTTGTGATAAGCGAAGTGTATTTCGGAAAACCATGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAGTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                        <  2:77305/238‑1 (MQ=255)
                                cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAAAAAAAAAAAAAACGGAGATGTCATTATGAGGGAAAGGAATAATAAACAGACAAGGAATAAATTgtc                                                                                                                                                                                                               >  2:884001/1‑237 (MQ=255)
                                     ttGTGTTAAGCTCGTTTTTTTTCCCAAAACTTTGCCATTCCCTTCTCTGGCGCAATATTCCTTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                          <  1:153035/239‑1 (MQ=255)
                                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAGTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                         <  2:319895/239‑1 (MQ=255)
                                          aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                                                                                                                                                     <  2:573673/223‑1 (MQ=255)
                                               cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGACAAAAACAAAAAATGGGGTTTGCCTGGTTAGGGGAAGTAATAAACAACAAGCAAGGGAAAAAATGACAGAGATTGTCGCTaa                                                                                                                                                                                               >  2:685558/1‑239 (MQ=255)
                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAACAGGAGGTGCCCTGATGAGCGGAAAGAATAAAAAACAAGCAGAGAAAAAGATGACAGAGATTGTGGCTAACTTTACCGCCATGGttt                                                                                                                                                                             >  2:497336/1‑239 (MQ=255)
                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAATAAAAAACTGGGGTTTCCCTGGTTGTCGGAAGTAATAAACTAAAAGCAGGGAAAAAAGTGAACAGGAATTGTGCTTACCTTTACCCCCagaatt                                                                                                                                                                              >  2:1320336/1‑234 (MQ=255)
                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCATACCAATAAAAAAAATATAGTTGCCCTGATTAGCGGAAGTAAAAAGAAACAAGCAAGGAAAAATATGACAAAAGATGTTGCTCACCTTACCTCCCTGAttt                                                                                                                                                                             >  2:851303/1‑239 (MQ=255)
                                                                  cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                            <  2:78684/179‑1 (MQ=255)
                                                                        cATTCCCTGCTTGGGCGCAATTTTCATTCTTATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCAGGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                     <  2:31256/239‑1 (MQ=255)
                                                                            cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATATTTTTCGACCAAAACAAAAAATGGAGTTGTCATGATGTGGGGAAGAAAAAAACAAACACAAGTGAATAAGATTACAAAGATTGTTGCTAATTTTATCCACCTCATTTCTTcccccaaca                                                                                                                                                                 >  2:1277090/1‑233 (MQ=255)
                                                                                 ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATGCAAGAACTGTTAATTACATATTCAGGGGGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                                                                                                                                                                         <  2:1451/226‑1 (MQ=255)
                                                                                            tatTCTTTTTGATTTTCGGTTTGATTAACGAACTGTTAATTACATATTGACGGGTGCAAAAAATATAAAACCACATGTTGAGTGTTATTTGGTGGCTGTGAATTTGTCCAGACAAATACAAAAGCGGGAGTTGCCATGATGAGGGAAAGTAATAAGCAACAGGAAGTGAAAAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                 <  2:1282646/239‑1 (MQ=255)
                                                                                            tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                     <  2:857478/160‑1 (MQ=255)
                                                                                                     ttATTTGCGGTTTGATTCACGAACTGTTAATTACATATTCCGGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                        <  2:1321586/239‑1 (MQ=255)
                                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGACAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  2:1155511/239‑1 (MQ=255)
                                                                                                      gggagatgtgtataaaggacagcTGTTAATTAAATATACAGGGAGAAAAAAAATAAAAACCACAATTTTTAGTGGTAGTTGGGGGGGGGAAATTTTTCAAGAAAAATAAAAAAAGTGGAGTTGCCATGAGGAGCGAAAGAAAAAAGCAACGGGCATAAAATAAGTGGACAGAGTTTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                       <  2:1233766/217‑1 (MQ=255)
                                                                                                           tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAAATGAGTTTCCATGATGAGCGGAAGAAATAAAAAACAAGCAGGGAATAAAGTTTCAGAGATTTTCGCTTACATTTACCCCCTGGTTTTTATCCGAAAGCCTGATGACGTGGGGGGtaaaataaaaaaact                                                                                                                                  >  2:406976/1‑234 (MQ=255)
                                                                                                                        cGATCTTTTAATTACATATTCAGGGTGACAAAAAATATAACACAACTTTTTTGGTGGTATTTGGTGGCGGTGATTTTTCCCAGACAAATAAAAAAACTGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCTTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                     <  2:131391/239‑1 (MQ=255)
                                                                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                  <  2:499028/155‑1 (MQ=255)
                                                                                                                                       ttttagggggaaaaaaaaaaTAAAAACCAATTTTTTGGGGGTTGTTGGGGGGGGTAAATGTTCCCAGAAAAAACAAAAAACGGGAGGTGCTGTGATGGGCAAAAGAAATAAGCAAGAGGAAGTAAATAGGTGGACAGATCTTGCCGCTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:884001/225‑1 (MQ=255)
                                                                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAAGGAGGTTGCCTGATGAGGGAAAAGAAAAAACAAAAGGCAGAGAATAAGATGAAAGAGAGTTATGGTAAATTTTCCGCCCTGAATTTTTCCCGAATGACTGCTGAAGTGGTGGGTGAAAAAAAACAACTAAAGGGTGCCGCAACAACGTCGTCgggg                                                                                                      >  2:836585/1‑239 (MQ=255)
                                                                                                                                        ttttccgggTGGAAAAAAAAAAAAACCAAAATTGTTGGTGGTAGTTGGGGGGGGTAAATGTTACAAGAAAAAAAAAAAAGTGGTGGTGGCCATGAAGAGCGAAATGAATAAGAACATGGCAGTGAATAAGTGGAAGGAGTTTGGCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:1320336/232‑1 (MQ=255)
                                                                                                                                        tttgtgggggggaaaaaatTAAAAAACCAAATTTTGGGGGGATTGTGGTGGGGGTAAATTTTCAAAAAAAAAAAAAAAAACGGGGTTGCCAAGAATGAAGAAATGTAAAAACAACAGGGAGTGTAATAGGTTGACAGATATTGTCGTAATTTTTACCGCCATGATTTCCACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  1:483071/226‑1 (MQ=255)
                                                                                                                                        ttgtCAGGGTAGAAAAAAATAAAAACCCAAATTTTAGGTGGTAGTTTGGGGCGTTGAATTTTACAAGAAAAAAAAAAAACCGGGAGTTGCATTGATGAGAAAAAGTAAAAAGCAACAGGAAGTAAATAATTTAAAAAAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:881361/236‑1 (MQ=255)
                                                                                                                                        tatTCAAGTTGGCAAAAAATAAAAACCCACATTTTGAGGAGTA‑‑TTGTGGTGGAGTAATTTTTCCCAGAGAAATACAAAAACGGGAGTTGCTAGGAGGAGCGAAAGTACAAAGCACCAGGCAGAAAATAAGTTAACAGAGATGCTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:1316716/239‑1 (MQ=255)
                                                                                                                                        atttccgggttgaaaaaaaTAAAAACCCCAATTTTAAGGGTTATTTTGGGGCGGTAAATTTTTCCAAACAAATAAAAAAACTGGGGTGGCAATGATGAGCAAAAGAAATAAGAAACGGGCAGTAAATAAGTTGACAGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:370095/226‑1 (MQ=255)
                                                                                                                                          ttCACGGTGACAAAAAATAAAAACCCAATGTTTGATGTGTAATTTGTGGCGGTAATTCTCAACAGAAAAAAAAAAAATGGGAAATGGCAATGATGTGCAAAAGAAAAAAGCAACAGGAATTGAATAAGTTGAAGGAGATTTAGTCTAAGTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                     <  2:1260178/237‑1 (MQ=255)
                                                                                                                                           tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAACTGGTGTTTCCATGGTTAGGGGAAAAGATAAAAAACAGGCAAGGGATAAAATGTAAGAGATTGTTGCTCACTTTTTCCCCCTGATTTCTACCCCCATGCCTGTTGAACTGGGGGAGAAACTAAAACAACTAAAAGAAGTCGAAAAGCCTCTGATGGGGgaaa                                                                                                  >  2:982406/1‑239 (MQ=255)
                                                                                                                                             cggggggaaaaaaaTATAAACCCCATTTTTAAGTGGTAGTTTGGGCAGGTGAATTTTTCAAGACAAATACAAAAACGGGATTTGCCATGATAACGGAAAGAAAAAAGCAACGGGCAGAAAATAGGTTAACAGGGATTGGCCCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                <  2:263352/231‑1 (MQ=255)
                                                                                                                                               ggTGGAAATAATTAAAAAACCATTTTTTTTGTGTTTGTTGCGGGTGGTTAATTTTTCCAGAAAAATGCAAAATGTGGAGTTGCCATGGTGGGGAAAAGTAATAAGCAAGAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  1:119881/239‑1 (MQ=255)
                                                                                                                                               aaaagagaaataTAGAAACCCACATTTTGAGTGGTATTTTGGGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                              <  2:99919/232‑1 (MQ=255)
                                                                                                                                                gTGGAAAAAAATAAAAAACACATTTTGTAGTGGTATTTTGGGGAGGAGAATTTGCCCAGAAAAAAAAAAAAACTGGATTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                <  2:573467/236‑1 (MQ=255)
                                                                                                                                                   gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATTGAGTTGCCCCGGTGAGCGGAAAGAATAAGCAAAAGGCAGGGAATAAGTTGAAAGAGAGTTTTGCTAAATTTACCCCCCTGATTTTTACCCGCATTGCTGTGTACGTGGTGGGTAAACTTAAAAAACTCAAGGATAGCCAAACGTAGTCGATGGGGGAAAATATCTa                                                                                           >  2:948919/1‑238 (MQ=255)
                                                                                                                                                   aaaaaaaaTAAAAACCCACTTTGTTGGGGTTATTTGGGGGCGGTAAATTTTTCAAAAAAAATAAAAAAACTGGGGTTGCCATGATGAGGGAAAGTAATAAGCAACGGGCAGTAAATAAGTTGACAGAGATTGGCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                           <  2:249766/236‑1 (MQ=255)
                                                                                                                                                         aaTATAAACCCCAATTGTTAGTGGTGATTTGGGTGGGTGAATTTTCCCAAAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                    <  2:498943/239‑1 (MQ=255)
                                                                                                                                                           aataAAACCCAATTTTTGAGGGGTATGTTGGGGGGGTGAATTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa                                                                                   <  2:816851/238‑1 (MQ=255)
                                                                                                                                                             aaaaaCCAGTTTTTAAGTGGTAGTTGGGGGGGGTAAATTTTCCAAGAAAAATAAAAAAACGGGAGTTGCAATGAGGAGAGAAAGTAAAAAGCAACGGGAAGTAAATAAGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGt                                                                                <  2:15825/238‑1 (MQ=255)
                                                                                                                                                                  ccACATTTTTAGTGGTATTTTGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                                                                                                                                                           <  2:982238/143‑1 (MQ=255)
                                                                                                                                                                  accatttttttAGGGTGTTTTGTGGGCGGGAATGTTTCCAAAAAAAAAAAAAAAATGGGAGTGTCAATAGTGAGGAAAAATAATAAGCACAGGTCAGTAAATAAGTAAAAGGGGTTTTCTCTAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                            <  1:982406/233‑1 (MQ=255)
                                                                                                                                                                        attttgtgtgtgttttttgggggCGGTAAATTTTCAAAAAAAAATAAAAAAAGTGGATTTGCTAGTATGAGCGAAATAAAAAAGAAACAGGGAGTAAATAAGTTGAAAGAGGTTGGAGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                         <  1:291498/218‑1 (MQ=255)
                                                                                                                                                                                      atttttTGGCGGTGAATTTATCAAGACAATATGACAAAGTGGGGTCACACTTATGAGAGAAATTAAATGACAACAGGCAGTGAATAAATAGACAGGGATTGTCTATTATTTTTCCGCCCTGATTTCTCCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:1192264/237‑1 (MQ=255)
                                                                                                                                                                                       tttttTGGGGGTTAAATTTTCCAAAAAAAAAACAAAAAATGGATTGGCATGATAAGGCAAAAGAAATGAGAAACGGTCAGTAAATATGTAGAAAGAGATGGTCGCTCATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTCAAGGATGCCGAAACGTCGGCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:800417/238‑1 (MQ=255)
                                                                                                                                                                                        tttttggggggtgtaaTTTTTCCAAAAAAAAACAAAAACGGGAGTTACCAGTGAGAGAAAAAGTAATAAGCAAGAGGAATGAAATAAGTGGACAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:138284/225‑1 (MQ=255)
                                                                                                                                                                                           gtgtCGGTGAATTGTTCCAAAAAAAAAAAAAAACGGGGGTTGGCAGATGGAGAGAAAGGAAAAACCAAAGGGGAGTAAATAAGTTGACGTGGATTGTCGCAAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                      <  1:234195/239‑1 (MQ=255)
                                                                                                                                                                                                            tttcaaaaaaaaTAAAAAAACGGGAGTGGCCATGATGAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  2:1291857/230‑1 (MQ=255)
                                                                                                                                                                                                               caaaaaaaaTAAAAAATTTGGAGTTGCCATGATGAGCAAAAGTAATAAACAACAGGCAGTGAATAATTTGACAGAGTTGCCCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                      <  2:998544/233‑1 (MQ=255)
                                                                                                                                                                                                               cAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                      >  2:737851/1‑238 (MQ=255)
                                                                                                                                                                                                                      aaTACAAAAACTGGATTTCCCATACGGGGCAAAAGTAATAAGCAACGGGCAGGGAATAGGTTGACAGGAATTGCCGCTACCTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                               <  2:624293/239‑1 (MQ=255)
                                                                                                                                                                                                                             aaaCTGGAGTTGCCATGATGAGCGACAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTCAAACAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCGCCCTGTCAgacacacggg                        >  1:350871/1‑230 (MQ=255)
                                                                                                                                                                                                                              agcTGGAGTTGCCAAGAAGGGCAAAAGTAAAAACAAAAGGGCAGTAAATAAGTTGAAAGAAATTGCCGCTATCGTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:750759/237‑1 (MQ=255)
                                                                                                                                                                                                                               aaggggAGTTGCATTGAGGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:350871/235‑1 (MQ=255)
                                                                                                                                                                                                                                    gAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCAAACGATGTTCGCCAACATGCAAAAAGGGATTGACTTGAATCGTCCTGCCTGTCAAGACACCGGGGGGAta                  >  1:1218750/1‑238 (MQ=255)
                                                                                                                                                                                                                                           gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGCGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGCAAATCATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCTTCCTGCCTGCCAGGACAACGGCAGGATTATGt              >  2:1179343/1‑238 (MQ=255)
                                                                                                                                                                                                                                              atgatgAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGGGATTCTGttct           >  2:1098907/1‑238 (MQ=255)
                                                                                                                                                                                                                                                          cGAAAGTAATAAGCAATAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTAGTCGATGGGGAAAATTATCTACCATACGAAGTTCTACAACAGCCAAAAAGCGATTGACCTGAATCATCCTGACTGCCGGGACACCGGGGGAATTATGTTCTTCGTTAAAg  >  1:867665/1‑239 (MQ=255)
                                                                                                                                                                                                                                                          cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAACGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTAACCTCAATCGCCCTGCCTGTCAGACAACCGGGGAGATTATGTTTTTCGTTAAAg  >  1:268898/1‑239 (MQ=255)
                                                                                                                                                                                                                                                          cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                               >  2:325370/1‑210 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   
TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206234‑3206707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: