Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,475 T→A 16.5% S5R (AGT→AGA ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4750TA16.5% 124.3 / 2.7 65S5R (AGT→AGAttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (13/35);  new base A (4/7);  total (21/44)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGG  >  NC_000913/3206241‑3206711
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
cccttttttacgctttttAATTGTCCGCTGTTGTGATAAGCGCAGTTTTTTTCACAACACATTGCCATTCCATGCACTGGAGAAATATTCATTCTTATTTTCGCATTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATGTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                                <  1:1216454/222‑1 (MQ=255)
cAGTTATTCTGCATGTTTAATTCTCCGCATTTGGGATAAGTCTAGTTTATTTCGCAAAACATTGCCATTCCCGGGTCTGGCGCAATATGCATTCTTATTGTCGCATTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                                <  1:39052/238‑1 (MQ=255)
cAGTTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                                <  2:677168/238‑1 (MQ=255)
cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                                <  2:191506/238‑1 (MQ=255)
cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                                <  2:599807/238‑1 (MQ=255)
          gtcttgTTAATTTGCCGCAGTTGTGCTAAGGGCAGTGTTTTTGCCAAAACATTGCCATTCCCTGCGCTGGCGCAATATTCATTCTTATTTTCGCGGTGATTCACGAACTGGTAAGTACATATTGACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                   <  1:416545/234‑1 (MQ=255)
          gctttttttattttcccggtttGGGAAAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                   <  1:48755/219‑1 (MQ=255)
           cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCATCCAAAATAAAAAATTGAGTTGTCATGATTAGGGGAAGAAAAAAACAAAAgaac                                                                                                                                                                                                                                   >  2:291498/1‑236 (MQ=255)
                 tAATTTTCCGCCGTTGTGATAAGCGCCTTTTATTTCTCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                                                                                                                                                           <  1:1247563/239‑1 (MQ=255)
                 tAATTATCCCCAGTTGTGATAAGCGAAGTGTATTTCGGAAAACCATGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAGTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                            <  2:77305/238‑1 (MQ=255)
                         cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAAAAAAAAAAAAAACGGAGATGTCATTATGAGGGAAAGGAATAATAAACAGACAAGGAATAAATTgtc                                                                                                                                                                                                                   >  2:884001/1‑237 (MQ=255)
                              ttGTGTTAAGCTCGTTTTTTTTCCCAAAACTTTGCCATTCCCTTCTCTGGCGCAATATTCCTTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                                                                                                                                                              <  1:153035/239‑1 (MQ=255)
                               tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAGTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                                                                                                                                                             <  2:319895/239‑1 (MQ=255)
                                   aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                                                                                                                                                         <  2:573673/223‑1 (MQ=255)
                                        cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGACAAAAACAAAAAATGGGGTTTGCCTGGTTAGGGGAAGTAATAAACAACAAGCAAGGGAAAAAATGACAGAGATTGTCGCTaa                                                                                                                                                                                                   >  2:685558/1‑239 (MQ=255)
                                                         aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAACAGGAGGTGCCCTGATGAGCGGAAAGAATAAAAAACAAGCAGAGAAAAAGATGACAGAGATTGTGGCTAACTTTACCGCCATGGttt                                                                                                                                                                                 >  2:497336/1‑239 (MQ=255)
                                                         aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAATAAAAAACTGGGGTTTCCCTGGTTGTCGGAAGTAATAAACTAAAAGCAGGGAAAAAAGTGAACAGGAATTGTGCTTACCTTTACCCCCagaatt                                                                                                                                                                                  >  2:1320336/1‑234 (MQ=255)
                                                         aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCATACCAATAAAAAAAATATAGTTGCCCTGATTAGCGGAAGTAAAAAGAAACAAGCAAGGAAAAATATGACAAAAGATGTTGCTCACCTTACCTCCCTGAttt                                                                                                                                                                                 >  2:851303/1‑239 (MQ=255)
                                                           cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                                <  2:78684/179‑1 (MQ=255)
                                                                 cATTCCCTGCTTGGGCGCAATTTTCATTCTTATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCAGGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                         <  2:31256/239‑1 (MQ=255)
                                                                     cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATATTTTTCGACCAAAACAAAAAATGGAGTTGTCATGATGTGGGGAAGAAAAAAACAAACACAAGTGAATAAGATTACAAAGATTGTTGCTAATTTTATCCACCTCATTTCTTcccccaaca                                                                                                                                                                     >  2:1277090/1‑233 (MQ=255)
                                                                          ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATGCAAGAACTGTTAATTACATATTCAGGGGGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                                                                                                                                                                             <  2:1451/226‑1 (MQ=255)
                                                                                     tatTCTTTTTGATTTTCGGTTTGATTAACGAACTGTTAATTACATATTGACGGGTGCAAAAAATATAAAACCACATGTTGAGTGTTATTTGGTGGCTGTGAATTTGTCCAGACAAATACAAAAGCGGGAGTTGCCATGATGAGGGAAAGTAATAAGCAACAGGAAGTGAAAAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                     <  2:1282646/239‑1 (MQ=255)
                                                                                     tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                         <  2:857478/160‑1 (MQ=255)
                                                                                              ttATTTGCGGTTTGATTCACGAACTGTTAATTACATATTCCGGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                            <  2:1321586/239‑1 (MQ=255)
                                                                                               tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGACAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                           <  2:1155511/239‑1 (MQ=255)
                                                                                               gggagatgtgtataaaggacagcTGTTAATTAAATATACAGGGAGAAAAAAAATAAAAACCACAATTTTTAGTGGTAGTTGGGGGGGGGAAATTTTTCAAGAAAAATAAAAAAAGTGGAGTTGCCATGAGGAGCGAAAGAAAAAAGCAACGGGCATAAAATAAGTGGACAGAGTTTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                           <  2:1233766/217‑1 (MQ=255)
                                                                                                    tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATACAAAAAAATGAGTTTCCATGATGAGCGGAAGAAATAAAAAACAAGCAGGGAATAAAGTTTCAGAGATTTTCGCTTACATTTACCCCCTGGTTTTTATCCGAAAGCCTGATGACGTGGGGGGtaaaataaaaaaact                                                                                                                                      >  2:406976/1‑234 (MQ=255)
                                                                                                                 cGATCTTTTAATTACATATTCAGGGTGACAAAAAATATAACACAACTTTTTTGGTGGTATTTGGTGGCGGTGATTTTTCCCAGACAAATAAAAAAACTGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCTTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                         <  2:131391/239‑1 (MQ=255)
                                                                                                                          aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                      <  2:499028/155‑1 (MQ=255)
                                                                                                                                ttttagggggaaaaaaaaaaTAAAAACCAATTTTTTGGGGGTTGTTGGGGGGGGTAAATGTTCCCAGAAAAAACAAAAAACGGGAGGTGCTGTGATGGGCAAAAGAAATAAGCAAGAGGAAGTAAATAGGTGGACAGATCTTGCCGCTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                         <  1:884001/225‑1 (MQ=255)
                                                                                                                                atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAAAGGAGGTTGCCTGATGAGGGAAAAGAAAAAACAAAAGGCAGAGAATAAGATGAAAGAGAGTTATGGTAAATTTTCCGCCCTGAATTTTTCCCGAATGACTGCTGAAGTGGTGGGTGAAAAAAAACAACTAAAGGGTGCCGCAACAACGTCGTCgggg                                                                                                          >  2:836585/1‑239 (MQ=255)
                                                                                                                                 ttttccgggTGGAAAAAAAAAAAAACCAAAATTGTTGGTGGTAGTTGGGGGGGGTAAATGTTACAAGAAAAAAAAAAAAGTGGTGGTGGCCATGAAGAGCGAAATGAATAAGAACATGGCAGTGAATAAGTGGAAGGAGTTTGGCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                         <  1:1320336/232‑1 (MQ=255)
                                                                                                                                 tttgtgggggggaaaaaatTAAAAAACCAAATTTTGGGGGGATTGTGGTGGGGGTAAATTTTCAAAAAAAAAAAAAAAAACGGGGTTGCCAAGAATGAAGAAATGTAAAAACAACAGGGAGTGTAATAGGTTGACAGATATTGTCGTAATTTTTACCGCCATGATTTCCACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                         <  1:483071/226‑1 (MQ=255)
                                                                                                                                 ttgtCAGGGTAGAAAAAAATAAAAACCCAAATTTTAGGTGGTAGTTTGGGGCGTTGAATTTTACAAGAAAAAAAAAAAACCGGGAGTTGCATTGATGAGAAAAAGTAAAAAGCAACAGGAAGTAAATAATTTAAAAAAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                         <  2:881361/236‑1 (MQ=255)
                                                                                                                                 tatTCAAGTTGGCAAAAAATAAAAACCCACATTTTGAGGAGTA‑‑TTGTGGTGGAGTAATTTTTCCCAGAGAAATACAAAAACGGGAGTTGCTAGGAGGAGCGAAAGTACAAAGCACCAGGCAGAAAATAAGTTAACAGAGATGCTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                         <  2:1316716/239‑1 (MQ=255)
                                                                                                                                 atttccgggttgaaaaaaaTAAAAACCCCAATTTTAAGGGTTATTTTGGGGCGGTAAATTTTTCCAAACAAATAAAAAAACTGGGGTGGCAATGATGAGCAAAAGAAATAAGAAACGGGCAGTAAATAAGTTGACAGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                         <  2:370095/226‑1 (MQ=255)
                                                                                                                                   ttCACGGTGACAAAAAATAAAAACCCAATGTTTGATGTGTAATTTGTGGCGGTAATTCTCAACAGAAAAAAAAAAAATGGGAAATGGCAATGATGTGCAAAAGAAAAAAGCAACAGGAATTGAATAAGTTGAAGGAGATTTAGTCTAAGTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                         <  2:1260178/237‑1 (MQ=255)
                                                                                                                                    tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAACTGGTGTTTCCATGGTTAGGGGAAAAGATAAAAAACAGGCAAGGGATAAAATGTAAGAGATTGTTGCTCACTTTTTCCCCCTGATTTCTACCCCCATGCCTGTTGAACTGGGGGAGAAACTAAAACAACTAAAAGAAGTCGAAAAGCCTCTGATGGGGgaaa                                                                                                      >  2:982406/1‑239 (MQ=255)
                                                                                                                                      cggggggaaaaaaaTATAAACCCCATTTTTAAGTGGTAGTTTGGGCAGGTGAATTTTTCAAGACAAATACAAAAACGGGATTTGCCATGATAACGGAAAGAAAAAAGCAACGGGCAGAAAATAGGTTAACAGGGATTGGCCCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                    <  2:263352/231‑1 (MQ=255)
                                                                                                                                        ggTGGAAATAATTAAAAAACCATTTTTTTTGTGTTTGTTGCGGGTGGTTAATTTTTCCAGAAAAATGCAAAATGTGGAGTTGCCATGGTGGGGAAAAGTAATAAGCAAGAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                  <  1:119881/239‑1 (MQ=255)
                                                                                                                                        aaaagagaaataTAGAAACCCACATTTTGAGTGGTATTTTGGGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                  <  2:99919/232‑1 (MQ=255)
                                                                                                                                         gTGGAAAAAAATAAAAAACACATTTTGTAGTGGTATTTTGGGGAGGAGAATTTGCCCAGAAAAAAAAAAAAACTGGATTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                    <  2:573467/236‑1 (MQ=255)
                                                                                                                                            gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATTGAGTTGCCCCGGTGAGCGGAAAGAATAAGCAAAAGGCAGGGAATAAGTTGAAAGAGAGTTTTGCTAAATTTACCCCCCTGATTTTTACCCGCATTGCTGTGTACGTGGTGGGTAAACTTAAAAAACTCAAGGATAGCCAAACGTAGTCGATGGGGGAAAATATCTa                                                                                               >  2:948919/1‑238 (MQ=255)
                                                                                                                                            aaaaaaaaTAAAAACCCACTTTGTTGGGGTTATTTGGGGGCGGTAAATTTTTCAAAAAAAATAAAAAAACTGGGGTTGCCATGATGAGGGAAAGTAATAAGCAACGGGCAGTAAATAAGTTGACAGAGATTGGCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                               <  2:249766/236‑1 (MQ=255)
                                                                                                                                                  aaTATAAACCCCAATTGTTAGTGGTGATTTGGGTGGGTGAATTTTCCCAAAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                        <  2:498943/239‑1 (MQ=255)
                                                                                                                                                    aataAAACCCAATTTTTGAGGGGTATGTTGGGGGGGTGAATTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa                                                                                       <  2:816851/238‑1 (MQ=255)
                                                                                                                                                      aaaaaCCAGTTTTTAAGTGGTAGTTGGGGGGGGTAAATTTTCCAAGAAAAATAAAAAAACGGGAGTTGCAATGAGGAGAGAAAGTAAAAAGCAACGGGAAGTAAATAAGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGt                                                                                    <  2:15825/238‑1 (MQ=255)
                                                                                                                                                           ccACATTTTTAGTGGTATTTTGTGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                                                                                                                                                               <  2:982238/143‑1 (MQ=255)
                                                                                                                                                           accatttttttAGGGTGTTTTGTGGGCGGGAATGTTTCCAAAAAAAAAAAAAAAATGGGAGTGTCAATAGTGAGGAAAAATAATAAGCACAGGTCAGTAAATAAGTAAAAGGGGTTTTCTCTAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                                <  1:982406/233‑1 (MQ=255)
                                                                                                                                                                 attttgtgtgtgttttttgggggCGGTAAATTTTCAAAAAAAAATAAAAAAAGTGGATTTGCTAGTATGAGCGAAATAAAAAAGAAACAGGGAGTAAATAAGTTGAAAGAGGTTGGAGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                             <  1:291498/218‑1 (MQ=255)
                                                                                                                                                                               atttttTGGCGGTGAATTTATCAAGACAATATGACAAAGTGGGGTCACACTTATGAGAGAAATTAAATGACAACAGGCAGTGAATAAATAGACAGGGATTGTCTATTATTTTTCCGCCCTGATTTCTCCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                               <  2:1192264/237‑1 (MQ=255)
                                                                                                                                                                                tttttTGGGGGTTAAATTTTCCAAAAAAAAAACAAAAAATGGATTGGCATGATAAGGCAAAAGAAATGAGAAACGGTCAGTAAATATGTAGAAAGAGATGGTCGCTCATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTCAAGGATGCCGAAACGTCGGCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                               <  1:800417/238‑1 (MQ=255)
                                                                                                                                                                                 tttttggggggtgtaaTTTTTCCAAAAAAAAACAAAAACGGGAGTTACCAGTGAGAGAAAAAGTAATAAGCAAGAGGAATGAAATAAGTGGACAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                               <  1:138284/225‑1 (MQ=255)
                                                                                                                                                                                    gtgtCGGTGAATTGTTCCAAAAAAAAAAAAAAACGGGGGTTGGCAGATGGAGAGAAAGGAAAAACCAAAGGGGAGTAAATAAGTTGACGTGGATTGTCGCAAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                          <  1:234195/239‑1 (MQ=255)
                                                                                                                                                                                                     tttcaaaaaaaaTAAAAAAACGGGAGTGGCCATGATGAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                             <  2:1291857/230‑1 (MQ=255)
                                                                                                                                                                                                        caaaaaaaaTAAAAAATTTGGAGTTGCCATGATGAGCAAAAGTAATAAACAACAGGCAGTGAATAATTTGACAGAGTTGCCCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                          <  2:998544/233‑1 (MQ=255)
                                                                                                                                                                                                        cAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                          >  2:737851/1‑238 (MQ=255)
                                                                                                                                                                                                               aaTACAAAAACTGGATTTCCCATACGGGGCAAAAGTAATAAGCAACGGGCAGGGAATAGGTTGACAGGAATTGCCGCTACCTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                   <  2:624293/239‑1 (MQ=255)
                                                                                                                                                                                                                      aaaCTGGAGTTGCCATGATGAGCGACAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTCAAACAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCGCCCTGTCAgacacacggg                            >  1:350871/1‑230 (MQ=255)
                                                                                                                                                                                                                       agcTGGAGTTGCCAAGAAGGGCAAAAGTAAAAACAAAAGGGCAGTAAATAAGTTGAAAGAAATTGCCGCTATCGTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                           <  2:750759/237‑1 (MQ=255)
                                                                                                                                                                                                                        aaggggAGTTGCATTGAGGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                           <  2:350871/235‑1 (MQ=255)
                                                                                                                                                                                                                             gAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCAAACGATGTTCGCCAACATGCAAAAAGGGATTGACTTGAATCGTCCTGCCTGTCAAGACACCGGGGGGAta                      >  1:1218750/1‑238 (MQ=255)
                                                                                                                                                                                                                                    gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGCGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGCAAATCATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCTTCCTGCCTGCCAGGACAACGGCAGGATTATGt                  >  2:1179343/1‑238 (MQ=255)
                                                                                                                                                                                                                                       atgatgAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGGGATTCTGttct               >  2:1098907/1‑238 (MQ=255)
                                                                                                                                                                                                                                                   cGAAAGTAATAAGCAATAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTAGTCGATGGGGAAAATTATCTACCATACGAAGTTCTACAACAGCCAAAAAGCGATTGACCTGAATCATCCTGACTGCCGGGACACCGGGGGAATTATGTTCTTCGTTAAAg      >  1:867665/1‑239 (MQ=255)
                                                                                                                                                                                                                                                   cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAACGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTAACCTCAATCGCCCTGCCTGTCAGACAACCGGGGAGATTATGTTTTTCGTTAAAg      >  1:268898/1‑239 (MQ=255)
                                                                                                                                                                                                                                                   cGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                   >  2:325370/1‑210 (MQ=255)
                                                                                                                                                                                                                                                       aGTAATAAGCACCAGGCAGTGAATAAGTTAACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                        <  1:187389/73‑1 (MQ=255)
                                                                                                                                                                                                                                                       aGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                        >  2:187389/1‑73 (MQ=255)
                                                                                                                                                                                                                                                       aGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg  >  2:192115/1‑239 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
CAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGG  >  NC_000913/3206241‑3206711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: