Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,504	C→A	16.5%	T15K (ACA→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,504	0	C	A	16.5%	163.0 / 4.7	72	T15K (ACA→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (26/32);  new base A (3/10);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206271‑3206740                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               ttGTGTTAAGCTCGTTTTTTTTCCCAAAACTTTGCCATTCCCTTCTCTGGCGCAATATTCCTTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAgaga                                                                                                                                                                                                                                           <  1:153035/239‑1 (MQ=255)  tgtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAGTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGAt                                                                                                                                                                                                                                          <  2:319895/239‑1 (MQ=255)           cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑TC‑TGACAAAAACAAAAAAT‑GG‑G‑GTTTGCC‑TG‑GT‑TA‑GGGGAAGTAATAAACAACA‑AG‑CAAGG‑GAAAAAATGACAGAGATTGTCGCT‑aa                                                                                                                                                                                                                                >  2:685558/1‑239 (MQ=255)                            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑TC‑CGACAAATACAAAAACA‑GG‑A‑GGTGCCC‑TG‑AT‑GA‑GCGGAAAGAATAAAAAACA‑AG‑CAGAG‑AAAAAGATGACAGAGATTGTGGCT‑AAC‑TTTACCGCCATGGttt                                                                                                                                                                                                              >  2:497336/1‑239 (MQ=255)                            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑TC‑CAACAAAATAAAAAACT‑GG‑G‑GTTTCCC‑TG‑GT‑TG‑TCGGAAGTAATAAACTAAA‑AG‑CAGGGAAAAAAGTGAACAGGAATTGTGCTT‑ACC‑TTTACCCCCagaatt                                                                                                                                                                                                               >  2:1320336/1‑234 (MQ=255)                            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AAATTTT‑TC‑ATACCAATAAAAAAAAT‑AT‑A‑GTTGCCC‑TG‑AT‑TA‑GCGGAAGTAAAAAGAAACA‑AG‑CAAGG‑AAAAATATGACAAAAGATGTTGCT‑CAC‑CTTACCTCCCTGAttt                                                                                                                                                                                                              >  2:851303/1‑239 (MQ=255)                                    cATTCCCTGCTTGGGCGCAATTTTCATTCTTATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCAGGGTGGCAAAAAATAAAAACCCAC‑ATTTT‑T‑AGTG‑GTAGTTTGGGGCGGTG‑AATTTTT‑CC‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGa                                                                                                                                                                                                      <  2:31256/239‑1 (MQ=255)                                        cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTT‑AATATTT‑TT‑CGACCAAAACAAAAAAT‑GG‑A‑GTTGTCA‑TG‑AT‑GT‑GGGGAAGAAAAAAACAAAC‑AC‑AAGTG‑AATAAGATTACAAAGATTGTTGCT‑AAT‑TTTATCCACCTCATTTCTTcccccaaca                                                                                                                                                                                                  >  2:1277090/1‑233 (MQ=255)                                             ctctGGCGAAATATTCATTCTTATTTTCGCGTTGATGCAAGAACTGTTAATTACATATTCAGGGGGGAAAAAAATAAAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑CA‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTAc                                                                                                                                                                                                          <  2:1451/226‑1 (MQ=255)                                                        tatTCTTTTTGATTTTCGGTTTGATTAACGAACTGTTAATTACATATTGACGGGTGCAAAAAATATAAAACCAC‑ATGTT‑G‑AGTG‑TTATTTGGTGGCTGTG‑AATTTGT‑CC‑AGACAAATACAAAAGCG‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GGGAAAGTAATAAGCAACA‑GG‑AAGTG‑AAAAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                  <  2:1282646/239‑1 (MQ=255)                                                                 ttATTTGCGGTTTGATTCACGAACTGTTAATTACATATTCCGGGGGGCAAAAAATAAAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑CC‑AGACAAATAAAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGAAAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                         <  2:1321586/239‑1 (MQ=255)                                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGACAAAAAATAAAAACCCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTA‑AATTTTC‑CC‑AGAAAAATAAAAAAACG‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                        <  2:1155511/239‑1 (MQ=255)                                                                  gggagatgtgtataaaggacagcTGTTAATTAAATATACAGGGAGAAAAAAAATA‑AAAACCACAATTTT‑T‑AGTG‑GTAGTTGGGGGGGGGA‑AATTTTT‑CA‑AGAAAAATAAAAAAAGT‑GG‑A‑GTTGCCA‑TG‑AG‑GA‑GCGAAAGAAAAAAGCAACG‑GG‑CATAA‑AATAAGTGGACAGAGTTTGTCCCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                        <  2:1233766/217‑1 (MQ=255)                                                                       tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑TC‑CGACAAATACAAAAAAA‑TG‑A‑GTTTCCA‑TG‑AT‑GA‑GCGGAAGAAATAAAAAACA‑AG‑CAGGG‑AATAAAGTTTCAGAGATTTTCGCT‑TACATTTACC‑CCCTGGTTTTTATCCGAAAGCCTGATGACGTGGGGGGtaaaataaaaaaact                                                                                                                                                                   >  2:406976/1‑234 (MQ=255)                                                                                    cGATCTTTTAATTACATATTCAGGGTGACAAAAAATATAACACAAC‑TTTTT‑T‑GGTG‑GTATTTGGTGGCGGTG‑ATTTTTC‑CC‑AGACAAATAAAAAAACT‑GG‑A‑TTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCTTT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                                      <  2:131391/239‑1 (MQ=255)                                                                                             aaTTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                                   <  2:499028/155‑1 (MQ=255)                                                                                                   ttttagggggaaaaaaaaaaTA‑AAAACCAA‑TTTTT‑T‑GGGG‑GTTGTTGGGGGGGGTA‑AATGTTC‑CC‑AGAAAAAACAAAAAACG‑GG‑A‑GGTGCTG‑TG‑AT‑GG‑GCAAAAGAAATAAGCAAGA‑GG‑AAGTA‑AATAGGTGGACAGATCTTGCCGCT‑ATT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                      <  1:884001/225‑1 (MQ=255)                                                                                                   atatTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑TC‑AGACAAATACAAAAAAA‑GG‑AGGTTGCC‑‑TG‑AT‑GA‑GGGAAAAGAAAAAACAAAA‑GG‑CAGAG‑AATAAGATGAAAGAGAGTTATGGT‑AAA‑TTTTCCGCCCTGAATTTTTCCCGAATGACTGCTGAAGTGGTGGGTGAAAAAAAACAACTAAAGGGTGCCGCAACAACGTCGTCgggg                                                                                                                                       >  2:836585/1‑239 (MQ=255)                                                                                                    ttttccgggTGGAAAAAAAAAAAAACCAAA‑ATTGT‑T‑GGTG‑GTAGTTGGGGGGGGTA‑AATGTTA‑CA‑AGA‑AAAAAAAAAAAGT‑GGTG‑GTGGCCA‑TG‑AA‑GA‑GCGAAATGAATAAG‑AACATGG‑CAGTG‑AATAAGTGGAAGGAGTTTGGCGTA‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                      <  1:1320336/232‑1 (MQ=255)                                                                                                    tttgtgggggggaaaaaatTAAAAAACCAA‑ATTTT‑G‑GGGG‑GATTGTGGTGGGGGT‑‑AAATTTT‑CA‑AAAAAAAAAAAAAAAAC‑GG‑G‑GTTGCCA‑AGAAT‑GA‑AGAAATGTAA‑AAACAACA‑GG‑GAGTGTAATAGGTTGACAGATATTGTCGTA‑ATT‑TTTACCGCCATGATTTCCACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                      <  1:483071/226‑1 (MQ=255)                                                                                                    ttgtCAGGGTAGAAAAAAATAAAAACCCAA‑ATTTT‑A‑GGTG‑GTAGTTTGGGGCGTTG‑AATTTTA‑CA‑AGAAAAAAAAAAAACCG‑GG‑A‑GTTGCAT‑TG‑AT‑GA‑GAAAAAGTAAAAAGCAACA‑GG‑AAGTA‑AATAATTTAAAAAAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                      <  2:881361/236‑1 (MQ=255)                                                                                                    tatTCAAGTTGGCAAAAAATAAAAACCCAC‑ATTTT‑G‑AGGA‑GTA‑‑TTGTGGTGGAGTAATTTTTCCC‑AGAGAAATACAAAAACG‑GG‑A‑GTTGCTA‑GG‑AG‑GA‑GCGAAAGTACAAAGCACCA‑GG‑CAGAA‑AATAAGTTAACAGAGATGCTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                      <  2:1316716/239‑1 (MQ=255)                                                                                                    atttccgggttgaaaaaaaTAAAAACCCCA‑ATTTT‑A‑AGGG‑TTATTTTGGGGCGGTA‑AATTTTT‑CC‑AAACAAATAAAAAAACT‑GG‑G‑GTGGCAA‑TG‑AT‑GA‑GCAAAAGAAATAAGAAACG‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGTT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                      <  2:370095/226‑1 (MQ=255)                                                                                                      ttCACGGTGACAAAAAATA‑AAAACCCA‑ATGTT‑T‑GATGTGTAATTTGTGGCGGTA‑ATTCTCA‑AC‑AGAAAAAAAAAAAATGG‑GA‑A‑ATGGCAA‑TG‑AT‑GT‑GCAAAAGAAAAAAGCAACA‑GG‑AATTG‑AATAAGTTGAAGGAGATTTAGTCT‑AAG‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                      <  2:1260178/237‑1 (MQ=255)                                                                                                       tCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑TC‑AAACAAAAACAAAAACT‑GG‑T‑GTTTCCA‑TG‑GT‑TA‑GGGGAAAAGATAAAAAACA‑GG‑CAAGG‑GATAAAATGTAAGAGATTGTTGCT‑CAC‑TTTTTCCCCCTGATTTCTACCCCCATGCCTGTTGAACTGGGGGAGAAACTAAAACAACTAAAAGAAGTCGAAAAGCCTCTGATGGGGgaaa                                                                                                                                   >  2:982406/1‑239 (MQ=255)                                                                                                         cggggggaaaaaaaTAT‑AAACCCC‑ATTTT‑TAAGTG‑GTAGTTTGGGCAGGTG‑AATTTTT‑CA‑AGACAAATACAAAAACG‑GG‑A‑TTTGCCA‑TG‑AT‑AA‑CGGAAAGAAAAAAGCAACG‑GG‑CAGAA‑AATAGGTTAACAGGGATTGGCCCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                                                 <  2:263352/231‑1 (MQ=255)                                                                                                           ggTGGAAATAATTAAAAAACCAT‑TTTTT‑T‑TGTG‑TTTGTTGCGGGTGGTT‑AATTTTT‑CC‑AGAAAAATGCAAAATGT‑GG‑A‑GTTGCCA‑TG‑GT‑GG‑GGAAAAGTAATAAGCAAGA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                               <  1:119881/239‑1 (MQ=255)                                                                                                           aaaagagaaataTAGAAACCCAC‑ATTTT‑G‑AGTG‑GTATTTTGGGGCGGTG‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                               <  2:99919/232‑1 (MQ=255)                                                                                                            gTGGAAAAAAATA‑AAAAACAC‑ATTTTGT‑AGTG‑GTATTTTGGGGAGGAG‑AATTTGC‑CC‑AGAAAAAAAAAAAAACT‑GG‑A‑TTTGCCA‑TG‑AT‑GA‑GCGAAAGTAAAAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTTTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                                                 <  2:573467/236‑1 (MQ=255)                                                                                                               gCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AAATTTT‑TC‑AAACAAAAAAAAAAAAT‑TG‑A‑GTTGCCC‑CG‑GT‑GA‑GCGGAAAGAATAAGCAAAA‑GG‑CAGGG‑AATAAGTTGAAAGAGAGTTTTGCT‑AAA‑TTTACCCCCCTGATTTTTACCCGCATTGCTGTGTACGTGGTGGGTAAACTTAAAAAACTCAAGGATAGCCAAACGTAGTCGATGGGGGAAAATATCTa                                                                                                                            >  2:948919/1‑238 (MQ=255)                                                                                                               aaaaaaaaTAAAAACCCAC‑TTTGT‑T‑GGGG‑TTATTTGGGGGCGGTA‑AATTTTT‑CA‑AAAAAAATAAAAAAACT‑GG‑G‑GTTGCCA‑TG‑AT‑GA‑GGGAAAGTAATAAGCAACG‑GG‑CAGTA‑AATAAGTTGACAGAGATTGGCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                                                            <  2:249766/236‑1 (MQ=255)                                                                                                                     aaTATAAACCCCA‑ATTGT‑T‑AGTG‑GTGATTTGGGTGGGTG‑AATTTTC‑CC‑AAAAAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GAGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGTTTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                                     <  2:498943/239‑1 (MQ=255)                                                                                                                       aataAAACCCA‑ATTTT‑TGAGGG‑GTATGTTGGGGGGGTG‑AATTTTC‑CC‑AGAAAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGa                                                                                                                    <  2:816851/238‑1 (MQ=255)                                                                                                                         aaaaaCCAG‑TTTTT‑A‑AGTG‑GTAGTTGGGGGGGGTA‑AATTTTC‑CA‑AGAAAAATAAAAAAACG‑GG‑A‑GTTGCAA‑TG‑AG‑GA‑GAGAAAGTAAAAAGCAACG‑GG‑AAGTA‑AATAAGTGGAAAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGt                                                                                                                 <  2:15825/238‑1 (MQ=255)                                                                                                                              ccAC‑ATTTT‑T‑AGTG‑GTATTTTGTGGCGGTG‑AATTTTT‑CC‑AGAAAAATAAAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCt                                                                                                                                                                                                            <  2:982238/143‑1 (MQ=255)                                                                                                                              accatttttt‑t‑AG‑G‑GTGTTTTGTGGGCGGG‑AATGTTT‑CC‑AAAAAAAAAAAAAAAATGGG‑A‑GTGTCAA‑TA‑GT‑GA‑GGAAAAATAATAAGC‑ACA‑GGTCAGTA‑AATAAG‑TAAAAGGGGTTTTCTCTAAAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGa                                                                                                             <  1:982406/233‑1 (MQ=255)                                                                                                                                    attttgtgtgtgttttttgggggCGGTA‑AATTTTC‑AA‑AAAAAAATAAAAAAAGT‑GG‑A‑TTTGCTA‑GT‑AT‑GA‑GCGAAATAAAAAAGAAACA‑GG‑GAGTA‑AATAAGTTGAAAGAGGTTGGAGAT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                                                                                                          <  1:291498/218‑1 (MQ=255)                                                                                                                                                  atttttTGGCGGTG‑AATTTAT‑CA‑AGACAATATGACAAAGT‑GG‑G‑GTCACAC‑TT‑AT‑GA‑GAGAAATTAAATGACAACA‑GG‑CAGTG‑AATAAATAGACAGGGATTGTCTAT‑TAT‑TTTTCCGCCCTGATTTCTCCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                            <  2:1192264/237‑1 (MQ=255)                                                                                                                                                   tttttggggggtgtaaTTTTT‑CC‑AAAAAAAAACAAAAACG‑GG‑A‑GTTACCAGTG‑A‑‑GA‑GAAAAAGTAATAAGCAAGA‑GG‑AATGA‑AATAAGTGGACAGAGATGGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                            <  1:138284/225‑1 (MQ=255)                                                                                                                                                   tttttTGGGGGTT‑AAATTTT‑CCAAAAAAAAAACAAAAAAT‑GG‑A‑‑TTGGCA‑TG‑AT‑AAGGCAAAAGAAATGAGAAACG‑GT‑CAGTA‑AATATGTAGAAAGAGATGGTCGCT‑CAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTCAAGGATGCCGAAACGTCGGCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                            <  1:800417/238‑1 (MQ=255)                                                                                                                                                       gtgtCGGTG‑AATTGTT‑CC‑AAAAAAAAAAAAAAACG‑GG‑G‑GTTGGCA‑‑G‑ATGGA‑GAGAAAGGAAAAACCAAAG‑GG‑GAGTA‑AATAAGTTGACGTGGATTGTCGCA‑AAT‑TTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                                       <  1:234195/239‑1 (MQ=255)                                                                                                                                                                       tttcaaaaaaaaTAAAAAAACG‑GG‑A‑GTGGCCA‑TG‑AT‑GA‑GCAAAAGTAAAAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGCT‑AAT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                          <  2:1291857/230‑1 (MQ=255)                                                                                                                                                                          caaaaaaaaTAAAAAATTT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCAAAAGTAATAAACAACA‑GG‑CAGTG‑AATAATTTGACAGAGTTGCCCGCT‑ACT‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                                                       <  2:998544/233‑1 (MQ=255)                                                                                                                                                                          c‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcct                                                                       >  2:737851/1‑238 (MQ=255)                                                                                                                                                                                 aaTACAAAAACT‑GG‑A‑TTTCCCA‑TA‑CG‑GG‑GCAAAAGTAATAAGCAACG‑GG‑CAGGG‑AATAGGTTGACAGGAATTGCCGCT‑ACC‑TTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                                                <  2:624293/239‑1 (MQ=255)                                                                                                                                                                                        aaaCT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGACAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTCAAACAGCTAAAGGATGCCGAAACGTCGTCGCTGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCGCCCTGTCAgacacacggg                                                         >  1:350871/1‑230 (MQ=255)                                                                                                                                                                                         agcT‑GG‑A‑GTTGCCA‑AG‑AA‑GG‑GCAAAAGTAAAAACAAAAG‑GG‑CAGTA‑AATAAGTTGAAAGAAATTGCCGCT‑ATC‑GTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  2:750759/237‑1 (MQ=255)                                                                                                                                                                                          aagggg‑A‑GTTGCAT‑TG‑AG‑GA‑GCAAAAGTAATAAGCAACA‑GG‑CAGTA‑AATAAGTTGACAGAGATTGTCGAT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  2:350871/235‑1 (MQ=255)                                                                                                                                                                                               g‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCAAACGATGTTCGCCAACATGCAAAAAGGGATTGACTTGAATCGTCCTGCCTGTCAAGACACCGGGGGGAta                                                   >  1:1218750/1‑238 (MQ=255)                                                                                                                                                                                                      gCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGCGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGCAAATCATCTACCATACGATGTTCGACAACATGCAAAAAGCGCTTGACCTGAATCTTCCTGCCTGCCAGGACAACGGCAGGATTATGt                                               >  2:1179343/1‑238 (MQ=255)                                                                                                                                                                                                         a‑tg‑at‑gA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGGGATTCTGttct                                            >  2:1098907/1‑238 (MQ=255)                                                                                                                                                                                                                     cGAAAGTAATAAGCAATA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTAGTCGATGGGGAAAATTATCTACCATACGAAGTTCTACAACAGCCAAAAAGCGATTGACCTGAATCATCCTGACTGCCGGGACACCGGGGGAATTATGTTCTTCGTTAAAg                                   >  1:867665/1‑239 (MQ=255)                                                                                                                                                                                                                     cGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAACGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTAACCTCAATCGCCCTGCCTGTCAGACAACCGGGGAGATTATGTTTTTCGTTAAAg                                   >  1:268898/1‑239 (MQ=255)                                                                                                                                                                                                                     cGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                                                >  2:325370/1‑210 (MQ=255)                                                                                                                                                                                                                         aGTAATAAGCACCA‑GG‑CAGTG‑AATAAGTTAACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                                                     <  1:187389/73‑1 (MQ=255)                                                                                                                                                                                                                         aGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                                                     >  2:187389/1‑73 (MQ=255)                                                                                                                                                                                                                         aGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                               >  2:192115/1‑239 (MQ=255)                                                                                                                                                                                                                            aataaGCAACG‑GG‑GGTTA‑AATATTTTGATAGAGTTTTCCGTT‑ATA‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                            <  1:685558/239‑1 (MQ=255)                                                                                                                                                                                                                              taaGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGACAGAAAACCGGAGAGATTATGTTCTTCGTTAAAGTAGGTGccc                          >  1:494328/1‑239 (MQ=255)                                                                                                                                                                                                                                aGCAACG‑GT‑GAGTA‑AATAATGTGACAGAGATGGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGc                        <  2:1211192/239‑1 (MQ=255)                                                                                                                                                                                                                                     cA‑GG‑CAGTG‑AATAAGTTGACATAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAGGGGGAAAATTATCTACCAACCGATGTTGGCCAACATCCAAAAACGGATTGACTTGAACCGGCACGCCTGCTAGGACACCGGGGGGATAATGTTTTTCGTTAAATTCTGTTCCCCCTGccc                   >  1:177933/1‑239 (MQ=255)                                                                                                                                                                                                                                     cA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGTCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                    >  2:808320/1‑238 (MQ=255)                                                                                                                                                                                                                                     cA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGCAACCGGGGAGAt                                                    >  2:858906/1‑206 (MQ=255)                                                                                                                                                                                                                                     cA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  2:670190/1‑239 (MQ=255)                                                                                                                                                                                                                                              tG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCtt            >  2:404763/1‑239 (MQ=255)                                                                                                                                                                                                                                                   tAAGTTGAAATGGTTTGTCGCA‑AAT‑TTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  2:769524/239‑1 (MQ=255)                                                                                                                                                                                                                                                   aaaGTTGACAGAAGTTGTCGTT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  2:1218750/238‑1 (MQ=255)                                                                                                                                                                                                                                                     aGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGGTTTCTACCCGAATGCCTGATGACGTGGTGGCTAAACTAAAACCGCTAAAGGATGCCGAAACGTCTTCGATTGGGAAAATTATCTACCAAACGATATTCGACAACATGCGAAAAGCAATTGACCTGAATCGTCCTGCCTGTCGGGACACCGGGCAGATAATTTTCTTCTTTGAAGTCAGTGCCCCCTTccccagcctggggcag      >  1:1198421/1‑226 (MQ=255)                                                                                                                                                                                                                                                     aGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCCACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACCGCTTGGCgg       >  2:475073/1‑237 (MQ=255)                                                                                                                                                                                                                                                         gACAGATATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                                     <  1:8516/124‑1 (MQ=255)                                                                                                                                                                                                                                                         gACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                                     >  2:8516/1‑124 (MQ=255)                                                                                                                                                                                                                                                         gACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAATTCGGTTCCCGCTTCCCCCTGCTTGGCGAGCTGc  >  2:985472/1‑239 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               TTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑T‑AGTG‑GTAGTTTGTGGCGGTG‑AATTTTT‑CC‑AGACAAATACAAAAACT‑GG‑A‑GTTGCCA‑TG‑AT‑GA‑GCGAAAGTAATAAGCAACA‑GG‑CAGTG‑AATAAGTTGACAGAGATTGTCGCT‑AAC‑TTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206271‑3206740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑