BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,340,103	G→C	11.0%	intergenic (‑40/‑170)	mlaF ← / → yrbG	ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein/putative calcium/sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,103	0	G	C	11.0%	280.3 / 2.9	109	intergenic (‑40/‑170)	mlaF/yrbG	ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein/putative calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand): ref base G (60/34); new base C (4/10); total (64/45)
Fisher's exact test for biased strand distribution p-value = 1.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 116 total aligned reads displayed.

AAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGCCGACCGCCTGG  >  NC_000913/3339865‑3340339

aaaggcttcccccttgctttggtggGATTGGCCCCCCAATTAGACGGGGTAGCGTCGTTTTCCCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCCCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGgcg                                                                                                                                                                                                                                                  <  1:808332/220‑1 (MQ=255)

aaaGGATCTCACCGTGATCTGGTGCCATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGgcg                                                                                                                                                                                                                                                  <  2:769541/239‑1 (MQ=255)

aaaGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGgcg                                                                                                                                                                                                                                                  <  2:258297/239‑1 (MQ=255)

aaaGGATCACACCATGCACTGGTTCGATTTGGCCGCCAATCAGACGGAGTAGCGTCGGTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGGCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGgcg                                                                                                                                                                                                                                                  <  2:744475/239‑1 (MQ=255)

              gggtgcgtgtggcttgccccGCAAAGCAGACGGAGGCGCGTCGTTTTACCGATGCCCGT‑TGGCCCCA‑TGACCGCCGTGATCTTCCCTCGCGGCACGTTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGGAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAccgt                                                                                                                                                                                                                                    <  1:804157/222‑3 (MQ=255)

               gATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAG‑‑‑CGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCCCAGACTGCTCCATAATTCACCCTT‑CGTCTTCCGTTGATTTTTTAAGAAGGGCGCTCATTTTAACCTTGaa                                                                                                                                                                                                                                 >  1:1169385/1‑238 (MQ=255)

                    gttgagtgtgccccGCCAACAGGACGGAGGAGCGTCGGTTCACCGAGGCCCGA‑TGGCCCCA‑TGATCGTCGGTATCTTCCCTCGCGACATGGTCATGGAAATATTATCGAAGATGCATCGATTGCCACGCGTAAAACG‑TACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAGTTAACCTTGAAccc                                                                                                                                                                                                                              <  1:894826/228‑1 (MQ=255)

                    ggTGCTATTTGCCCGCCAATCAGGCGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAccc                                                                                                                                                                                                                              <  2:22904/239‑1 (MQ=255)

                    ggTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCCGCGATTGCCACGCGTAAAAGC‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACGAACTGCTCCATAATTAACCCTT‑CGTCTTTCGTTTATTTTCTAAACATGGCCCTCAATTTAACCTTAAAccc                                                                                                                                                                                                                              >  1:298942/1‑239 (MQ=255)

                    ggTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGTGCCCAATTTAACCTTGAAccc                                                                                                                                                                                                                              >  2:1106321/1‑239 (MQ=255)

                    ggTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAccc                                                                                                                                                                                                                              >  2:886420/1‑239 (MQ=255)

                     gTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCCCGCGAAAACCT‑GACA‑TCCGGCATAT‑CGACTAAATTCGCCACAGACTGCTCCTTAATTAACCCTT‑CGTCTTTCGTTGATTTTCTAAGCACGGCGCCCAATTTAACCCTGAACCC‑a                                                                                                                                                                                                                            >  1:1273605/1‑239 (MQ=255)

                      tGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGC‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGTGAAATATTCTCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCCCCCTT‑CGTCTTGCGTTTATTTTCTAAGCATGTCGATCAATTTAACCTTTAAACC‑a                                                                                                                                                                                                                            >  2:990240/1‑238 (MQ=255)

                      tGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGCTCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGCTGCACCGCTTGCCACGCGTAAAACT‑GTCA‑TCGCGCATAA‑CGCCTAAATCCGCCACAGCCTGCTCCATAATTCACCCTT‑CGTATTGCGTTGATTTTCTAAGCATGGCGACCAATTTAAACTTTAACCC‑a‑a                                                                                                                                                                                                                          >  1:1322115/1‑239 (MQ=255)

                      tGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑a‑a                                                                                                                                                                                                                          >  2:196930/1‑239 (MQ=255)

                      tGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑a‑a                                                                                                                                                                                                                          >  2:49831/1‑239 (MQ=255)

                      tGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACACACTGCTCCATAATTAACCCTT‑CGTCTTGCGTTGATTTTCTCACCATGGGCCTCAATTTAACCTTAAACCC‑a‑a                                                                                                                                                                                                                          >  1:22904/1‑239 (MQ=255)

                      tGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGACCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑a                                                                                                                                                                                                                            >  2:59155/1‑238 (MQ=255)

                      gccctttgcccccccAATCAGCCGGAGTAGCCTCGTTTTACCGGTGCCCGA‑AGGCCCCT‑TGACCGCCGTGATCTTCCCGCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑a‑a                                                                                                                                                                                                                          <  1:1053734/230‑1 (MQ=255)

                       gctttttCCCCCCCAATCAGACGGGTTAGCTTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGGTCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGATTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑a‑a                                                                                                                                                                                                                          <  1:504035/234‑1 (MQ=255)

                          aTTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTTCCACGCGTAAAACT‑GGCA‑TCACGCATAT‑CGACTAAATTTCCCACAAACTGCCCCCTAATTCACCCTT‑CGTCTTCCGTTGTTTTTCTAAGCATGGCGCCCCATTTAACCTTACACCC‑A‑ACaat                                                                                                                                                                                                                      >  1:990989/1‑237 (MQ=255)

                           ttctcccGCCATTCAGTCCGGGTAGCCTCCTTTTACCGCTGCGCGG‑TGGCCCCT‑TGAACGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCGCCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACatat                                                                                                                                                                                                                     <  1:725234/235‑1 (MQ=255)

                              gCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTa                                                                                                                                                                                                                  <  2:383031/239‑1 (MQ=255)

                              cccccccAATCTGGCGGAGTAGCGGCGTTTTCCGCGTGCCCGT‑AGGCCCCA‑AGATCGCCGGTATCTTCCCCCGCGGCACGGTCAGGGAAATATTATTGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTG‑CGGCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTa                                                                                                                                                                                                                  <  1:1018850/238‑1 (MQ=255)

                                ccgccaaggtggcGGTGTTGCGTCGTTTAACCGTCGCCCGA‑TGGCCCCA‑GGATCCCCGTGATCTTCCCTCGCGGCACGGTCAGGAAAATATTATCGCAGATGCAGCGATTGCCACGCGTAAAACT‑AACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACa                                                                                                                                                                                                                <  1:265634/227‑1 (MQ=255)

                                ccccctatacggggggttggggtcgtTTTACCGAGGCCCGA‑TGGCCCCA‑TGATCGCCGTGAGCTTCCCCCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGGCTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACa                                                                                                                                                                                                                <  1:301310/218‑1 (MQ=255)

                                ccGCCAATCGAGCGGAGTAGCGGCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATGGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACa                                                                                                                                                                                                                <  2:1153450/239‑1 (MQ=255)

                                ccGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAA‑TCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACa                                                                                                                                                                                                                <  2:173598/238‑1 (MQ=255)

                                ccGCCAAGCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACa                                                                                                                                                                                                                <  2:1268557/239‑1 (MQ=255)

                                               gttggtgtgtttttACCG‑TGCCCCAGTGGCCCCA‑GGGTCGCCGGGTTTTCCCCGCGGGGCACGGACAGAGAAATATTAGCGAAGATGGAGCGTTTGCCACTCATAAAACT‑AGCA‑CGCCGCTGAC‑CGACTAAATTCCCCACAGACTGCTCCATAATTCCCCCTT‑CGTCTGGCGTTGATTTTCTAAGCATGGCGCCCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACccgcc                                                                                                                                                                                                 <  1:953621/228‑1 (MQ=255)

                                                     gtcgtTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GCCA‑TCGCGCATAT‑CGACTAAATTCGCCCCAGCCTGCTCCATACTTCACCCTT‑CGCCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACACATTTACAG‑AAAATTAACCCCCGTgttat                                                                                                                                                                                          >  1:535316/1‑235 (MQ=255)

                                                        tttttttccggccccGA‑GGGCCCCACTGATCGCGTGGATTTGCCCCCGGGGCAGGGATGGGGAAATCTTAAGGAATTAGCGTCGATGGCCACGATAAAAAGA‑GACA‑CCGCAGATAT‑CGGAAAAATCCGCCACAGACTGCTCAATATTCCACCCTT‑CGTCTTGCTTTTTTTTGCTAAGCATGGCCCTCAATTTAACCATAAACCC‑A‑AAATATTTACAG‑AATATTACCCGCCGTGGTTA‑Gc                                                                                                                                                                                       <  1:1327653/227‑1 (MQ=255)

                                                         ttttACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGGTCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGACGATGCAGCGCTTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAGTTTAAc                                                                                                                                                                                                                                       >  2:1071858/1‑193 (MQ=255)

                                                         ttttACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACCTATTTCCGA‑CATATTACCCGCCGTGGTTA‑GCGa                                                                                                                                                                                     >  2:133084/1‑239 (MQ=255)

                                                         ttttACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGa                                                                                                                                                                                     >  2:922855/1‑239 (MQ=255)

                                                         ttttACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGa                                                                                                                                                                                     >  2:168291/1‑239 (MQ=255)

                                                                   gCCCGA‑TGGCCCCA‑TGATCGCCTTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACa                                                                                                                                                                                                                <  1:7011/204‑1 (MQ=255)

                                                                             ccccA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTACCCTTGAACCC‑A‑CCATATTTACTG‑AATATTACCCGCCCCGGTTA‑TGTAAAGCTGGCATTTGtttt                                                                                                                                                                    >  2:570482/1‑237 (MQ=255)

                                                                                      tcgcggggattgtCCCCCGCGCCAGCGTCTGGAAAATATTGTCGAAGATTAAGGTATTGCCACGCGAAAAAC‑‑GTCACTCCGTAATAT‑CGCATAATTTCTCCACGGATCGCTCCAAAATTCCCCTGC‑CGTCTTGATTTGATTTTCAAAACATGGCCCGCAATTAAACC‑TGAACCCAA‑ACATTTTGACTG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTTCTTTTTAGc                                                                                                                                                          <  1:743590/227‑1 (MQ=255)

                                                                                       cGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GTGAAACCTGGCATTTGTTTTACTTTTTAGcc                                                                                                                                                         >  2:743590/1‑239 (MQ=255)

                                                                                          cGTGATCTTCCCTCGCGGCACGGTCAGGCAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCTCGCATAT‑CGACTAAATTCTCCACAGACTGCTCCTTAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCAGTGCGCTCAATTTTACCTTGAACCC‑ACACA‑CTTTACGT‑AATATTCACCGCCGTGGTTACTCTAAAAC‑GGCATTTGTTATATTTTTTatctgcc                                                                                                                                                      >  1:682070/1‑233 (MQ=255)

                                                                                               tCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTACGCATGGCGCTCAATTTAACCTTGAACCC‑C‑ACATATTTACAG‑ATTATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTATTTTTTACCCCCAAAAAg                                                                                                                                                 >  1:1293720/1‑239 (MQ=255)

                                                                                                ttcccccCGGGGCAGTGTCAGGGAAATATTACTGAAAGTGCAGCGTTTGCCGCGCTTAAATCT‑AACC‑TCGCGAATAT‑CACCTAAATTCCCCACAAACGGCTCCATAATTCCCCCTT‑CGTTTTGCTTTGATTTTTTAAGCTGGGCGCTCAATTTAACCTTAAACCC‑A‑ACATATTTCCAG‑ATTATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGt                                                                                                                                                <  1:365136/236‑1 (MQ=255)

                                                                                                gttttaaaggacagctgTCAGGAAAATTTGACCAAAGTTGACGCCATTCCCCCGCTAAAAACT‑GACA‑TCGCGAATAT‑CGCCTAAATGCGCCACAACCTGCCCCATAATCACCCCTT‑CGTCTTGCGTTTATTTTCTAAGCATGCCGCTCAATTTAACCTTAAACCC‑A‑CCATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGt                                                                                                                                                <  1:966886/223‑1 (MQ=255)

                                                                                                 ttCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGGGGTTA‑GCGAAAGCTGGAATTTGATTTACTTTTTAGCCGCATAAAGTc                                                                                                                                               >  2:457973/1‑239 (MQ=255)

                                                                                                   taaaagagcgggggTCAGAAAAATATTTTCAAAGGTGAAGCGATTGCCAGCGGTAAAACT‑GACA‑CCCCGCTTAT‑CGTAAAATTTCCCCACAGACTTCTCAAAAATTCCCCCTT‑CGTCTGGCTTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATACTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCaa                                                                                                                                             <  1:510286/227‑1 (MQ=255)

                                                                                                      tCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACCTATTTACAG‑AATATTACCCCCCGTGGTTA‑CAGAAAACCAGCCTTTTTTTTACTTTTTCACCGCATAAACTCaaaaa                                                                                                                                          >  1:532109/1‑238 (MQ=255)

                                                                                                             cgggTCAGGAAAATTTTAACAAAGATCCAGCGATTCCCA‑GCGGAAAAATCTAAA‑TCGCGAATAT‑CGAATAATTTCGCCAAAGACTGCCCCATAATTCCCCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTACCCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCa                                                                                                                                    <  2:1169385/236‑1 (MQ=255)

                                                                                                                         tttttttctgaagtgcacgttttGCCCCGCTAAAAACT‑TACA‑CCGCGTATAT‑CGAATAAATTCGCCAAAGACTGTCCAAAAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTAAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCtt                                                                                                                       <  1:1110659/218‑1 (MQ=255)

                                                                                                                         atatTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAg                                                                                                                                      >  2:966886/1‑224 (MQ=255)

                                                                                                                          tattatCGACGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AAAATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTAAAAATTAAGAAACCGTTAAGGGttt                                                                                                                      >  1:580004/1‑239 (MQ=255)

                                                                                                                          tattatCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCAt                                                                                                                                   >  2:510286/1‑226 (MQ=255)

                                                                                                                           attatCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCtt                                                                                                                       >  2:524498/1‑237 (MQ=255)

                                                                                                                           attatCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCAGCCGTGGTTA‑TCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCTTTCCGGCattt                                                                                                                     >  2:378528/1‑238 (MQ=255)

                                                                                                                               tCGAAGATGCAGCGATTGCCACGCGTAAAACT‑TACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAccc                                                                                                                                                                                                                              <  1:1156928/134‑1 (MQ=255)

                                                                                                                               tCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAccc                                                                                                                                                                                                                              >  2:1156928/1‑134 (MQ=255)

                                                                                                                               tCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGaa                                                                                                                 >  2:494199/1‑239 (MQ=255)

                                                                                                                                    gATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTCCTTTTTACCCGCATACAGTCAAAATTAAGCATCCGTTACGTCTTTCTGaaaactt                                                                                                            >  1:376546/1‑236 (MQ=255)

                                                                                                                                      tGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTc                                                                                                          >  2:190641/1‑239 (MQ=255)

                                                                                                                                               tGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCg                                                                                                 >  2:271662/1‑239 (MQ=255)

                                                                                                                                                gCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑aa                                                                                                                                                                                                            >  2:975725/1‑132 (MQ=255)

                                                                                                                                                gCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑aa                                                                                                                                                                                                            <  1:975725/132‑1 (MQ=255)

                                                                                                                                                 ccACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGc                                                                                               >  2:358870/1‑239 (MQ=255)

                                                                                                                                                  cgcgcgTAAAACG‑GACA‑TCGCGC‑TATGCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCTCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAA‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGc                                                                                               <  2:376546/236‑1 (MQ=255)

                                                                                                                                                  cagggttAAACCT‑GACT‑TCGGCAATAT‑CGACTAAATTCCCCCCAGCCTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTACCCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGc                                                                                               <  2:1183952/232‑1 (MQ=255)

                                                                                                                                                      ggaaaaaTT‑GACA‑TCCCGTATAC‑CGACTAAATTCGCCAAAGCCTGCTCCATAATTCCCCCTT‑CGTCTTGCGTTGTTTTTCTAAGCAGGGCGCCAAATTTAACCTTGAACCC‑A‑AAATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGta                                                                                          <  2:990989/236‑1 (MQ=255)

                                                                                                                                                      cgTAAACCT‑GACA‑TCTCCCATAC‑CGATTAAATTCGCCAAAGACTGCCCCATAATTCCCCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGACCCC‑A‑ACATATTTACAG‑AATTTTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGta                                                                                          <  2:218028/239‑1 (MQ=255)

                                                                                                                                                         aaaaCT‑GACA‑TC‑CGCATTTACGACTAAATTCCCCAAGGACTGCCACTAAATTCCCCCTT‑CGTCTTGGTTTGATTTTCTAAGAATGGCCCTAAATTTAACCTTGAACCA‑A‑ACATATTTCCAG‑AATATTCCCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATAc                                                                                       <  2:1273605/239‑1 (MQ=255)

                                                                                                                                                                gACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTTTGAAAATCTTAAGCGGACCGGCGAGAAAACCGgaag                                                                                 >  1:1219790/1‑239 (MQ=255)

                                                                                                                                                                  cA‑TCGCGCATAT‑CGAATAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTAACCCTTGACCCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTgaaga                                                                                <  2:1219790/238‑1 (MQ=255)

                                                                                                                                                                      ctctctttttcccttAATTTCGCCACCGCCTGCTCCAAAATTCCCCCTT‑CGTTTTGCTTTGTTTTTTAAAGCATGGCCCTAAATTTACCCTTGAACCC‑A‑ACTTTTTTACAA‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAg                                                                             <  1:922855/225‑1 (MQ=255)

                                                                                                                                                                        cgcATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTCCGGCTTTCTGAAAATCTTCAGCGGACCGCCGAGTATACCTGAAGAAAgg                                                                            >  2:793010/1‑237 (MQ=255)

                                                                                                                                                                         gcATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGGCACAATTAAGCATCCGTTCCGGCTTTCTGAAAATCTTTAGCGGACCGGCGAGTCTACCTGAAGAAAGGACg                                                                         >  2:615844/1‑239 (MQ=255)

                                                                                                                                                                               tggctattttctcccccaGTCTGCTCTAAAATCCACCCTT‑CGTCTTGCTTTTTTTTTCTAAGCTTGGCCCTCAATTTAACCTGGAACCC‑A‑ACATGTTTACAG‑AATTTTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAATGGACGTTAGa                                                                    <  1:524498/223‑1 (MQ=255)

                                                                                                                                                                               actaCTAAATTCGCCACGGACGTCCCCATAATCGCCCCTT‑GGTTCTGCGTTGATTTTCTAGTCATGGGGCTCAATTGACCCTTAACCCA‑A‑ACATATTTACAT‑ATGATTCACCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTTGa                                                                    <  2:664261/236‑1 (MQ=255)

                                                                                                                                                                                  aCTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAAGACGTTAGAt                                                                   >  2:467146/1‑237 (MQ=255)

                                                                                                                                                                                    tAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTCCGGCTTTCTGAAAATCTTCAGCGCACCGGCGAGTATACCGTAAGAAAGCAAGTTAGATGAtt                                                               >  1:815639/1‑239 (MQ=255)

                                                                                                                                                                                            ccagccgtctccccAAAACTCCCCCTTGCGTCTTGCTTTGTTTTTCAAAGCTGGGCGCACAATTTAACCTGAAACCC‑A‑ACATTTTTACAA‑AATATTCACCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTa                                                        <  1:273746/227‑1 (MQ=255)

                                                                                                                                                                                             cacaGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAACCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTTCAG‑ACTATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATCAATTCAAAATTAAGCATCAGTTACGGCTTTCTGAAAACCTTCAGCGGACCGGCTAGTAGACTTGAAGAAAGGAGGTTAAATACTTTTAGCCAcc                                                      >  1:885640/1‑238 (MQ=255)

                                                                                                                                                                                               cagcatGCCCCATAATTCCCCCTT‑CGTCTTGGCTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATTTTTACAG‑AATATTCCCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGc                                                    <  2:393571/234‑1 (MQ=255)

                                                                                                                                                                                                   cggCTCCATAGTTCCCCCTG‑CGTCTGGCTTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGACCCA‑A‑ACATATTTACAG‑AATTTTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCa                                                   <  2:1322115/234‑1 (MQ=255)

                                                                                                                                                                                                   cTGCCCCATATTTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCCCTCAATTTAACCTTAAACCC‑A‑ACATATTAACAA‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCa                                                   <  2:868585/236‑1 (MQ=255)

                                                                                                                                                                                                              ttCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATACAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTAAACTTGAAGAAAGGCCGTTAGAGGCTTTTGACTACGGACCGGTTAATTGTTgg                                     >  1:1128377/1‑239 (MQ=255)

                                                                                                                                                                                                                cACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCCCTGTTAAttg                                         >  2:576897/1‑233 (MQ=255)

                                                                                                                                                                                                                cACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCCCTGTTAATTGTTGGtt                                   >  2:643007/1‑239 (MQ=255)

                                                                                                                                                                                                                 aCCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGAGGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGttt                                  >  2:75038/1‑239 (MQ=255)

                                                                                                                                                                                                                   ccTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGACAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGATTTTAACTACGGCCCTGTTAATTGTTGGttt                                  >  2:500552/1‑237 (MQ=255)

                                                                                                                                                                                                                   ccTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGCCCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTTTTGGttt                                  >  2:1299994/1‑237 (MQ=255)

                                                                                                                                                                                                                     tt‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAg                                                                     <  2:361090/200‑1 (MQ=255)

                                                                                                                                                                                                                     tt‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAg                                                                     >  1:361090/1‑200 (MQ=255)

                                                                                                                                                                                                                       tgtttttGCGTGGATTTTCTAAGCAGGGCGCTCAATTTAACCTGGAACCA‑A‑ACAAATTTACAG‑AATATTCCCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACttt                             <  2:1293720/234‑1 (MQ=255)

                                                                                                                                                                                                                        cGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACtttt                            >  2:146757/1‑239 (MQ=255)

                                                                                                                                                                                                                         tccttgtttttgtttgtaaaGCATGCCGTTAAATTTAACCTTAGACAC‑A‑ATATTTTTAAGG‑ATTATTACCCGCCGTGGTTA‑GCGAAAGCTGGCAATTGTTTTACTTTTTAGCCGCATAAAGTCAAAAGTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTg                           <  1:1276993/221‑1 (MQ=255)

                                                                                                                                                                                                                         gTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGGATACCTGAAGAAAGGACTTTAAATGCTTTTAGTTACGGACCTGTTAATTGTTGGTTTTCTTTTg                           >  1:535515/1‑239 (MQ=255)

                                                                                                                                                                                                                            ttGTGTTGTTTTTCTAGGCATGGCCCTAAATTTAACCTTGAACCC‑A‑ACATTTTTACAA‑AATTTTCCCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTc                        <  2:535316/239‑1 (MQ=255)

                                                                                                                                                                                                                             ttggtgttttttttaaaagtGGCCCTTAAATTTACCCTGAACCC‑C‑AAATTTTTTTAAAAATTTTCCCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTGCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTc                        <  1:271662/220‑1 (MQ=255)

                                                                                                                                                                                                                              gCGTTGATTTTCTAAGCATGGCGCTCAATTTCACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGGCAAAATTACGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGCCCGGCGCGTATACCTGAAGAAAGGGCGTTAGATGCTTTTGGCTCAGGCCCTGTTAATTGTTGGTTTACTTTTgg                          >  2:297968/1‑235 (MQ=255)

                                                                                                                                                                                                                                tttatttttCTAAGCAGGCCCCTAAATTTACCCTGGACCCC‑A‑AAATATTTACAA‑AATTTTCCCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGttt                    <  2:535515/234‑1 (MQ=255)

                                                                                                                                                                                                                                          tAAGCGTGGCGCATAATTTTACCTTTAACCC‑ATACTTTTTTAAAG‑AATTTCCCCCGCCGTGGTTA‑GCGAAATCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGGCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATTCCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGCCg           <  1:146757/239‑1 (MQ=255)

                                                                                                                                                                                                                                            aGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTAAGTCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCCGAAGAAAGGGCGTTAAATGTTTTTAACTACGCCCCTGTTATTTTTTGGTTTACTTTTTGTCGTTTTAAGTGCCAAcc        >  1:579694/1‑239 (MQ=255)

AAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGA‑TGGCCCCA‑TGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACT‑GACA‑TCGCGCATAT‑CGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTT‑CGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCC‑A‑ACATATTTACAG‑AATATTACCCGCCGTGGTTA‑GCGAAAGCTGGCATTTGTTTTACTTTTTAGCCGCATAAAGTCAAAATTAAGCATCCGTTACGGCTTTCTGAAAATCTTCAGCGGACCGGCGAGTATACCTGAAGAAAGGACGTTAGATGCTTTTAGCTACGGCACTGTTAATTGTTGGTTTACTTTTGGTCGTTTACAGTGCCGACCGCCTGG  >  NC_000913/3339865‑3340339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`