Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,364,405	C→A	11.6%	S534* (TCA→TAA)	yhcD →	putative outer membrane fimbrial subunit usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,364,405	0	C	A	11.6%	237.8 / 2.0	83	S534* (TCA→TAA)	yhcD	putative outer membrane fimbrial subunit usher protein
Reads supporting (aligned to +/- strand):  ref base C (61/11);  new base A (2/9);  total (63/20)
Fisher's exact test for biased strand distribution p-value = 2.11e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CTTGGTTTAAACTG‑GTCTAACTCCATTCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAGGTG  >  NC_000913/3364176‑3364641                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              cTTGGTTTAAACTG‑GTCTAACTCCATTCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCg                                                                                                                                                                                                                                      >  2:1016715/1‑238 (MQ=255)   tGGGTTAAACCGTGTCTAACCCAATTAGGGGGGGTTTCAACATCAGTGGCTAAAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTA‑AATATAAACGGG‑AACAAAGCTTTCAAA‑CC‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTAAAAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGACCAAC‑CACCGcc                                                                                                                                                                                                                                    <  2:1090284/239‑1 (MQ=255)    gggttaaaactgtacaaccccaaTTCGGGGTGGTTTCAACACCAGGGACTAAAAAACATAGAGTTCCGCGGGTGAAAATGCCTAAAGCAACTTT‑AATATTAACTGG‑AACAAGGC‑TTCAAACCA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAAAAAAT‑AATGAAGCCGATGGTAA‑T‑C‑TTTTCT‑ACGTCAACATCAGTATTCCATTTGGAAGATCAAA‑CACCGCca                                                                                                                                                                                                                                   <  2:783361/217‑1 (MQ=255)    ggTTTAAACTG‑GTCTAACTCCATTCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGCAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCACCATCAGTATTCCATTTGGCAGATCAAA‑CACCGCca                                                                                                                                                                                                                                   >  2:990134/1‑238 (MQ=255)    ggTTTAAACTG‑GTCTAACTCCATTCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGGGCTTCAGGACAT‑AATGAAGACGATGCTGA‑T‑C‑TGTTCA‑ACGTAAACATCAGTATTCCATATGGAAAATCAAA‑CACCGCca                                                                                                                                                                                                                                   >  2:990133/1‑238 (MQ=255)     gTTTAAACTT‑GTCAAACCTCATTGTGGGTGTTTCCAACATCAGTGGCTAAAAAAAATAATGTTACGAAGGTGACAATGAC‑CAAACAAACTTAAATATTAACTGA‑AAAAAAGCGTTAAAA‑CA‑CGCCACG‑G‑TTC‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCTTTTGGCAGATCAAA‑CACCGCcaca                                                                                                                                                                                                                                 <  2:471542/239‑1 (MQ=255)                gTCTAACTCCATTCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGATCAAA‑CA‑CTCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGCATTCCAATTGTCAAATCAAA‑AACACCCACACTgaaaaca                                                                                                                                                                                                                        >  1:752924/1‑232 (MQ=255)                gTCTAACTCCATTCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGAATCCAGCCACCTT‑AATATTACCTGG‑AACAACGCGTTCAAA‑CA‑CGCAACG‑G‑TTT‑CCGCCAACTGGCTGCATCAACTTAGCGCTTCAGAAAAT‑AATGACGACGCTGGGGA‑C‑C‑TGTTCT‑ACGTCAAATTCAGTATTCACTTTGCCAAGCCAAA‑CACACCcaccagattaaaa                                                                                                                                                                                                                        >  1:1195189/1‑228 (MQ=255)                           ttCTGGGTGGTTTCAACATCAGTGGCTATAACACATATAGTTACGACGTTGATCATGACTTAAGCATCCTT‑AATATTAATTGG‑AACAAAGCGTTCAAA‑CA‑CGCCCCG‑G‑TTT‑CCGCTAACAGGATGCATCTACTTAGTGCTTCAGAAAAT‑AATAAACACGCTGGTTC‑T‑C‑TGTCTT‑ACACACACCTCAGGATTCCATTTCGAAGATCAAA‑AACCTCCACACTgataaccccccataacgaa                                                                                                                                                                                                            >  1:139280/1‑221 (MQ=255)                           ttCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAAGGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAACCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACATAAAAATCAGAATTCCATTTGGCAAATCAAA‑AACCGCCACACTgaaaaacaccaatacaaaa                                                                                                                                                                                                            >  1:882427/1‑221 (MQ=255)                                ggtggtTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑CTT‑CCGTCAACAGGCAGCATCAACTTATTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑A‑C‑TGTTCT‑ACGTAAACATCAGTATTCCATTTGGCAGATCGAA‑CACCGCCACACTGAATACTCGCCATGAcaaacaaaa                                                                                                                                                                                                       >  1:318440/1‑231 (MQ=255)                                  gttttcaaaCTTCAGTGGCTAAAAAAAGTATAGTTACGACGGTAAAAATGCCTCAATCAACTTA‑AATATTAACTGA‑AACAAGCCGTTAAAA‑CA‑CGCAGGG‑G‑TTT‑CCTTAACCGGGCAGCATCAACTGTGGCCTATAAAAAAT‑AATAAATACGATGGGAA‑T‑T‑TTTTCT‑CCGTCAACAGAAGTATCCTTTTTGGCAGAAAAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATaaa                                                                                                                                                                                                      <  2:318440/231‑1 (MQ=255)                                     tttCAACATCAGGGGTTAAAAAACATATAGTTACGAGGGTGACAATGACTCAAGCAACTTT‑AATATGAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGAAGCATAAATTTAGTGTTAAAAAAAAT‑AATGAAGCCGATGGTGA‑T‑C‑TGTTCT‑ACGAAAACATCAGTATTCCTTTTGGAAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAAcc                                                                                                                                                                                                   <  2:159566/238‑1 (MQ=255)                                       taaaataaagggccTAAAAAAAATATATTAACGAGGGGAAAAAAGACT‑AAAAAACCTT‑AAAATTAACTGG‑AAAAAAG‑GTTTGCA‑CA‑CCCCCCGAG‑TTTCCCGTAACCTGGCACCAACAACTTGATGCTTAAAAAAAA‑AATGAAGACGAGGGTAA‑T‑C‑TGTTTT‑CCGTAAACACCGTTTTTCCTTTTGGCAGATAAAC‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCAc                                                                                                                                                                                                <  1:942768/226‑1 (MQ=255)                                         aaCATCGGAGGATAAAAAACATAATGTAAGGGCGGTAAAAATCACTAAAGCAACGTT‑ATTATTAACGGA‑AACAAGGTGTAAAAA‑CC‑CGCCACG‑T‑TTT‑CCGTAAACTGGCACCATCATTTTAGTGCTTCAGAAAAA‑AATGAAGACGATTGTGA‑T‑T‑TGTTCT‑ACATCATAAACATTATTCCATTTGGAAGATAAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTa                                                                                                                                                                                              <  2:139280/239‑1 (MQ=255)                                               aGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTCTGGAAc                                                                                                                                                                                        >  2:1024377/1‑239 (MQ=255)                                               aGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGAATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTAc                                                                                                                                                                                        >  2:326181/1‑239 (MQ=255)                                                 tGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATAATAAAACCCACTATGGAACTg                                                                                                                                                                                      >  2:221919/1‑239 (MQ=255)                                                   gCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAC‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgt                                                                                                                                                                                    >  2:919932/1‑239 (MQ=255)                                                               ataGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CTCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCATCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACACCAGCATTCAATTTGGCAGATAAAC‑CACCGCCAAACTGAATACTCCCCATAACCATCCTAAAACCAACAATggcaatgcacaacaagaaaag                                                                                                                                                                        >  1:1256543/1‑220 (MQ=255)                                                               ataGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtc                                                                                                                                                                        >  2:405453/1‑239 (MQ=255)                                                               ataGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtc                                                                                                                                                                        >  2:709747/1‑239 (MQ=255)                                                               ataGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGATTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATAAAA‑CACCGCCACACTGTATACTCGCCATTACGACCATAAAACCCACTATGGAATCGGGGTAATGGGAg                                                                                                                                                                          >  2:1218728/1‑237 (MQ=255)                                                               ataGTTACGACGGGGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGCCGATGGTGA‑T‑C‑TGTTCT‑ACTTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGACCCACTGGATACTCGCCATGACGATCATAAAACCCACCATGGAACAGGGTTCAAggggaag                                                                                                                                                                        >  1:1116688/1‑235 (MQ=255)                                                                     acgacgGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAGAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGCCATGGGACTCGTCTCa                                                                                                                                                                  >  2:447407/1‑239 (MQ=255)                                                                      cgacgGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCAAACTGTATACTCGCCATCACGACAATAAAACCCCCAAAGGAACACGAGCCATGGGAgacggtccaa                                                                                                                                                                 >  1:637010/1‑233 (MQ=255)                                                                         cgGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGtata                                                                                                                                                                                                                          >  2:1091597/1‑179 (MQ=255)                                                                           gTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCagatgag                                                                                                                                                            >  2:542467/1‑239 (MQ=255)                                                                           gTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATAATAAAACCCACTATGGCACCGGTGTCATGGGAatcggcccgacagca                                                                                                                                                            >  1:367794/1‑228 (MQ=255)                                                                            tgaaaatacccaaaacAACCTG‑AATA‑TGACTGGAAACAAAGCGTAGAAA‑CC‑AGCCACG‑C‑GTT‑CCCACAAGGGGCACCTCAAACTTAGTGATTCAAAAAAA‑AAGAGAGCGAATGGTCA‑T‑T‑TGGTCT‑CAGACAAAATAAATATTCCATTTGAAAGAACAGA‑CCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCagatgag                                                                                                                                                            <  1:1186115/223‑1 (MQ=255)                                                                            taaCAATGACCAAAGCACCCTT‑AATATTAACGGA‑AAAAAACCTTTAAAA‑CC‑CG‑CACG‑GTTTT‑CCGTCACCTGGCAGCAACACCTTAGTCCTTAAAAAAAT‑AATGAAGACGTTGGTGA‑T‑C‑TGTTCT‑ACGTCAACACCAGTATTCTTTTTGGAAAATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCagatgag                                                                                                                                                            <  2:860923/236‑1 (MQ=255)                                                                             ttaactgaccccacacatcTT‑TATTTTAAATGG‑AAAAAAGTGTTCAAA‑CACCGCCAGT‑G‑TTT‑CCGAAAATTGGCACATTCAACTTAGT‑CTTTAAAAAATAAATAAAGGCGATGGT‑A‑T‑CTTTTTCT‑ACTTAAACATCATTATTCCTTTTGGAAAACCAAC‑CCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCagatgag                                                                                                                                                            <  1:608411/220‑1 (MQ=255)                                                                             gACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCagatgagat                                                                                                                                                          >  2:216473/1‑239 (MQ=255)                                                                                          gCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTAtgtg                                                                                                                                             >  2:908895/1‑239 (MQ=255)                                                                                              ccTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCTTAAAACCCACTATGGTACTGGAGTCATGGGGGTCGTCTCAGATGAGATTTCCTACTATGTGAATg                                                                                                                                         >  2:53383/1‑239 (MQ=255)                                                                                              ccTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACAATGTGAAt                                                                                                                                          >  2:642375/1‑238 (MQ=255)                                                                                                   aaTATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGa                                                                                                                                     >  2:22938/1‑239 (MQ=255)                                                                                                    atatTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTCCTGGTGTCATGGGAGTCGTCTCCGATGGAATGTCCTACTATGTGAATGCTGa                                                                                                                                     >  1:58808/1‑238 (MQ=255)                                                                                                    atatTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATACTAAAACCCACTCTGGTACTGGGGTCATGGGGTACGTCTCAGACGAGAAGTCcaactcatgaaattcttaa                                                                                                                                    >  1:402782/1‑225 (MQ=255)                                                                                                     attaaaCGGG‑AACAAACCTTTCAAA‑CC‑CGCCACG‑G‑TTC‑CCACCAACTGGAAGCATAAATTTAGTCCTTAAAAAAAA‑AAGGAAGACG‑TGTGGACT‑C‑TTTTCT‑CCGTCAACAATATTTTTCCATTTGGCAGATAAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAAc                                                                                                                                   <  2:58808/235‑1 (MQ=255)                                                                                                         aaCTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCAt                                                                                                                                                                               >  2:235906/1‑191 (MQ=255)                                                                                                          aCTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACgaga                                                                                                                               >  2:426017/1‑238 (MQ=255)                                                                                                          aCTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCACCTATGGAACTGGGGTCATGGGAGTCGTCTCAGATAAGATGTCCAACTAATTGAATGCTGAAGGAAAt                                                                                                                              >  1:395402/1‑239 (MQ=255)                                                                                                          aCTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGCCAACTGGCAGCATCCACTTAGCGCTTCAGAAAAT‑AATCACGCCGATGTTGA‑T‑C‑TGTTCT‑CCCTCAACATCAGAATTCCATTTGGCAGATCACA‑CACCTCCACACTGTATACCCGCCATGAAGATCCTCAAAACCACTATGCGACTGGAGCCATAGAAGCCGCACCAGGTGAGCTGTCCAACACTGTGAATGCCGAACgaga                                                                                                                               >  1:524255/1‑238 (MQ=255)                                                                                                                aaCAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCacg                                                                                                                          >  2:1304065/1‑238 (MQ=255)                                                                                                                      acgggcgtcaaaaccccccagttttt‑CCCTCAACGGGAAACAAAAACTTGGGGCTTAAAAAAAA‑AATGAAGACGAGGGTGA‑C‑T‑TTTTCT‑CCGTCAACATCAGTATTCCTTTTGGCAAATAAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCacgacg                                                                                                                       <  2:479655/216‑1 (MQ=255)                                                                                                                          tCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTa                                                                                                                                                                                         >  2:806495/1‑165 (MQ=255)                                                                                                                          tCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGGGTCATGGGAGTCGGCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACAACGAACGGGaa                                                                                                               >  2:693840/1‑239 (MQ=255)                                                                                                                           cAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGaa                                                                                                               >  2:93530/1‑238 (MQ=255)                                                                                                                                       tgg‑g‑TTT‑CCGAAAACTGCAACCATCAACTTTGTCCTTAAAAAAAT‑AATGAAGACGGGGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCATTATTCCATTTGAAAGAAAAAC‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGaa                                                                                                    <  2:882427/237‑1 (MQ=255)                                                                                                                                                   gtaaaCTGGCAGCATCAACTTAGGGCTTCAGAAAAT‑AATGAAGGCGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATCCTTTTGGGCAGATAAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTAt                                                                                           <  2:395402/236‑1 (MQ=255)                                                                                                                                                        cTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGt                                                                                       >  2:331092/1‑238 (MQ=255)                                                                                                                                                                aTCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGGGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGGGAAACGGGCTTGAAAGGcgagacaagttcacatccc                                                                              >  1:486888/1‑221 (MQ=255)                                                                                                                                                                         gttctaaaaaaaaT‑AAGGAAGACGAT‑TTGT‑TCC‑TTTTCT‑ACGTAAACAACAGTTTTCATTTTGACATATAAAA‑CCCCGCCACACTGTTCACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACAcc                                                                     <  1:542467/230‑1 (MQ=255)                                                                                                                                                                         gTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTacac                                                                      >  2:803293/1‑238 (MQ=255)                                                                                                                                                                          tGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGATTCGTCTCAGATGAGATGTCCTACTATGGGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTAGAACGGCAGTAACAATTCCAAACCCCATTaaaacc                                                                    >  1:67031/1‑234 (MQ=255)                                                                                                                                                                               caaaaatT‑AATAAAGCCGGTGGTGA‑‑‑C‑TGTTCTCACGTCAAAATCAGCATTCCATTTGGCAGTACAAA‑CCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTc                                                               <  2:67031/236‑1 (MQ=255)                                                                                                                                                                               cAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAgcc                                                               >  2:423918/1‑237 (MQ=255)                                                                                                                                                                               cAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTc                                                               >  2:1141075/1‑239 (MQ=255)                                                                                                                                                                               cAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTAATGGGAGTCGTCTCATATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGCCGAACGGGAAACAAGCTTGCACGGCAGGAACAGATCCAATTGCCAATAAACCCaaaga                                                               >  1:633374/1‑236 (MQ=255)                                                                                                                                                                               cAGAAAAT‑AAGGAAGACGATCGTGA‑T‑C‑TGTTCT‑ACGGCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTTGTGTCATGGCAGTCGTCTCAGATTAGATGTCCTACTATGTGAATGCTGAACGAGATACCGACGAACGGAAAACGGACTTGACCGTCACGATCAATACCAATCTCCATTAAACACAAgaa                                                               >  1:1002266/1‑237 (MQ=255)                                                                                                                                                                                      t‑aatGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGGGTCATGGGAGTCGTCTCAGATGAGCAGACCTACTATGAGAATGCTGAACGAGATCACGACTAACGTGAAACAGCCTAGAACAGCGATACCCATTCCAATCTCCAGAACACCAAAGACAgaatag                                                        >  1:361669/1‑234 (MQ=255)                                                                                                                                                                                                   tGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGGGAAACGAGCTTGAACGGCATAATCAGTTCCAACCCCCATTCAAACCAAATCAGCCTTGCCGCAgagacaa                                            >  1:500012/1‑233 (MQ=255)                                                                                                                                                                                                   tGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGAACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGCGAATGCTGAACGAGATCACGACGACCGGACAACGAGCTTGAACGGAAGTATCAGTTCCAATCTCCATTACAacccgtctcttatacacatttcccac                                             >  2:753434/1‑215 (MQ=255)                                                                                                                                                                                                          t‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGACCGGCAGTATCAGTTCCAACCTCCATTACACCCAAGTCAGCCTTGCCGCCAGAGAAACCGGCa                                      >  1:529087/1‑239 (MQ=255)                                                                                                                                                                                                            c‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAg                                     >  2:1157862/1‑239 (MQ=255)                                                                                                                                                                                                             ttggtttcacataaacaacaacgttCCATTTGGACAAGCAAAGCCCCGGCACACAGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCgg                                        <  2:1223657/215‑1 (MQ=255)                                                                                                                                                                                                                ttCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGGGCAACGGGCAGGGa                                  >  2:33024/1‑239 (MQ=255)                                                                                                                                                                                                                ttCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTAAACCCAAGGCAGCCTTGCCGCAGGAGCAAGCGGCAgcaa                                  >  2:1312014/1‑236 (MQ=255)                                                                                                                                                                                                                ttCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGGGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAAAGGCAGTATACGTTCCAATCTCCATTACACCCAAATCACCCTTGCCGCCGGAACAAGCGGCAGGGa                                  >  1:961965/1‑239 (MQ=255)                                                                                                                                                                                                                      cctaaacatatgtagtctttttGGCAGAAAAAA‑CCCCGCCACACTGTATACCCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGcc                             <  2:402782/220‑1 (MQ=255)                                                                                                                                                                                                                      cGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGcc                             >  2:70320/1‑239 (MQ=255)                                                                                                                                                                                                                           aCATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAAc                                                                                                             >  2:562226/1‑154 (MQ=255)                                                                                                                                                                                                                           aCATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACt                        >  2:40738/1‑239 (MQ=255)                                                                                                                                                                                                                           aCATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTAAACCCAAGCCAGCCTTTCCCCAGGAGCAAGCGGCatggtaaaccgaact                        >  2:609602/1‑225 (MQ=255)                                                                                                                                                                                                                                  tATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGa                                                >  2:247053/1‑208 (MQ=255)                                                                                                                                                                                                                                       tttttGGCAAAACAAA‑CCCCGCCACACTTTATAACCGCCATGAAGGTCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACg            <  1:70320/237‑1 (MQ=255)                                                                                                                                                                                                                                       cATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGCACTTACAACGGTCCg            >  2:1276/1‑239 (MQ=255)                                                                                                                                                                                                                                         tttGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGAt          >  2:54587/1‑239 (MQ=255)                                                                                                                                                                                                                                          ttGGCAGATCAAA‑CACCGCCACACTGTATGCTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAg                                               >  2:446694/1‑201 (MQ=255)                                                                                                                                                                                                                                            ggCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCctac                                                                                                                                                   >  2:393597/1‑99 (MQ=255)                                                                                                                                                                                                                                            gccAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCctac                                                                                                                                                   <  1:393597/97‑1 (MQ=255)                                                                                                                                                                                                                                                 aTCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAgggg  >  2:1065919/1‑237 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              CTTGGTTTAAACTG‑GTCTAACTCCATTCTGGGTGGTTTCAACATCAGTGGCTATAAAACATATAGTTACGACGGTGACAATGACTCAAGCAACCTT‑AATATTAACTGG‑AACAAAGCGTTCAAA‑CA‑CGCCACG‑G‑TTT‑CCGTCAACTGGCAGCATCAACTTAGTGCTTCAGAAAAT‑AATGAAGACGATGGTGA‑T‑C‑TGTTCT‑ACGTCAACATCAGTATTCCATTTGGCAGATCAAA‑CACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTCGTCTCAGATGAGATGTCCTACTATGTGAATGCTGAACGAGATCACGACGAACGTGAAACGAGCTTGAACGGCAGTATCAGTTCCAATCTCCATTACACCCAAGTCAGCCTTGCCGCAGGAGCAAGCGGCAGTGATAGCCGTACTTACAACGGTACGATGTCAGGTG  >  NC_000913/3364176‑3364641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑