BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	319,100	T→A	5.0%	Y301F (TAC→TTC)	rclA ←	reactive chlorine stress species (RCS) resistance protein, pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	319,100	0	T	A	5.0%	305.7 / 2.1	104	Y301F (TAC→TTC)	rclA	reactive chlorine stress species (RCS) resistance protein, pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand): ref base T (75/22); new base A (3/3); total (79/25)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 109 total aligned reads displayed.

CGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTT‑CATGGTGACTGATTCGCTCCAC  >  NC_000913/318862‑319338

cggttttAATCCCCCAAGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATgt                                                                                                                                                                                                                                                       <  2:1039324/236‑1 (MQ=255)

cGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATgt                                                                                                                                                                                                                                                       <  2:95539/239‑1 (MQ=255)

             ccACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGTGGCGGGGTCATAAATACGGCATAAGGCACATTTTTCCGATCCTCCGTACTACGTTTGCCTTCCCCCCGTAACCCCTCACCTACCATGCCGTAAACATCCACGGATATTTAAAAAAATTGCac                                                                                                                                                                                                                                          >  1:1064080/1‑238 (MQ=255)

             ccACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCATTGATATGGAAGTAAATTGCa                                                                                                                                                                                                                                           >  2:164041/1‑238 (MQ=255)

                        attcatCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATTCGGTAATCTTCCAGGGATATGTAAGTAAATTGCATCCCG‑CCGGTac                                                                                                                                                                                                                              >  2:834646/1‑238 (MQ=255)

                         ttcatCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCCCATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACCATGCGGTAATCATCCAGGGATTAGTAAGTTAATTGAAGCCCG‑CCGGCAAc                                                                                                                                                                                                                             >  2:206830/1‑239 (MQ=255)

                            atcaCTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGCTTGCCTTCACCCAGTAAATCATCACGTACAATGCGGTAATCATCCAATGATATgtaagtctgtcttttttaaccatttccta                                                                                                                                                                                                                            >  2:1030606/1‑215 (MQ=255)

                              caCTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGCATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACAtat                                                                                                                                                                                                                         >  2:419600/1‑236 (MQ=255)

                                   tGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGCGTCATAAATAAGGAATAAGGCACATTTTTCCGATCATCCGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATTCGGGAATCATCCAGTGATATTTAAGTCAATTTCAACCCT‑CCGGTAAAATCTCCCAt                                                                                                                                                                                                                    >  2:369371/1‑238 (MQ=255)

                                    ggCCCGGGGAATTGCAGTTACAGGCAATGTCCCCCCCGTAATTTCAGCCCCAC‑CTCTTCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGTCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCg                                                                                                                                                                                                                  <  2:153110/239‑1 (MQ=255)

                                        cgcgGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGAACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCAAGTACAATGCGGTAATCATCCCGGGATATGTAAGTAAATTTAAGCCCC‑CCGGGAAAAACACCCATCGCCCa                                                                                                                                                                                                              >  1:296264/1‑239 (MQ=255)

                                             aaTTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATgt                                                                                                                                                                                                                                                       <  2:419460/194‑1 (MQ=255)

                                               tggtagcttgaGGAAATGTCATCACCAAAATAGCGACCCCACTCCC‑ACTGTCTTGCTCTCCTGTCATACCAACTCTGGACAGGGGCGGTGTCATAAATTCGTAAACAGGACCATTTTCCCGATCAAAAGTACTACGTTTGCCTGGACGCAGAAACCAATCACGAAAAATCCGGTAATCACCCAGTGATATGAAATGAAATTGCGGCCCG‑CCGGTAACATCTCCCATCGCCCAAATAtt                                                                                                                                                                                                        <  1:365069/228‑1 (MQ=255)

                                                   ttattagagacgggTCCCCCCCTGAATCTCAGCCCCCCTCTT‑TCGGGCTTTTTTTTTTGTCATCACACCCCTGGAAGGGGGCGGTGTCATAACTCCGGAATAAGGCACATTTTTCCGATCATCGGTACTACGTTTGCCTTCACCCAGTAACTCATCACTTACAATGCGGTAATCACCCGGTGATATGTAAGTAAATGGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTg                                                                                                                                                                                                  <  1:1050349/226‑1 (MQ=255)

                                                      ttcAGGCAATTCCACCCCCCAAATATCCGCCCAACTCTC‑TTGTGGTTGTTCCTTCGTCATCCCACCCCCGGACAGGCGCGGTCGCAGAAATACGGAAAAAGGCCCATTTTTCCGATCATCAGTACTTCGTTCGCCTTCCCACAGTAACTAATCACGTACCATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTg                                                                                                                                                                                                  <  1:879531/234‑1 (MQ=255)

                                                      taaaGGCAATGTCCCCCCCTGAATATCAGCACCCCTCTC‑TTTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTTTCATAAATTCGAAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCCTAACCCAGTAACTCATCACGTTCAATGCGATAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCgg                                                                                                                                                                                               <  2:786764/236‑1 (MQ=255)

                                                      tACGGGCAATGGCACCACCGAAATACCGGCCCCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCACCCCTGGACAGGGGCGGTGTCAAAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTCCGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCg                                                                                                                                                                                                <  2:231989/238‑1 (MQ=255)

                                                       aCAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGATTGCCTTCAACCAGTAACTCATTACGTACAATGCGGGAATCATCCAGTGATATTTAAGTAAATTGCAACCCG‑CCGGTAAAAAACACCAACGCCCAAAAAtttggtggggt                                                                                                                                                                                               >  1:646823/1‑230 (MQ=255)

                                                       aCAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAAGCCCATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAAATTATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATAGCCCAAATATTT‑TCTGCggg                                                                                                                                                                                              >  2:518417/1‑238 (MQ=255)

                                                       aCAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAAGCCCATTTTTCCGAACATCAGTACTACGTTTGCCTTCACACGGTAACTCATAACGTACAATGAGGGAATAATCCAGTGATATGAAAGTAAATTg                                                                                                                                                                                                                                             >  1:419460/1‑194 (MQ=255)

                                                       aCAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGGCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCATTAACACGTCACGTCCAATCCGGTAATCATCCCTTGATATGTAAGTAAAATGCAGCCCG‑CCGGCACCATCACCCATCGCCCAAAAAtttacggccgg                                                                                                                                                                                               >  1:1251868/1‑230 (MQ=255)

                                                               tGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGGATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCCCCCAGTACATCATCACGTACAATAGGGGAAACATTCACTGGTATGTAAGTAAATTTCAACCCGCCCGGCAAATTCCCCCACCG‑CCAAAAAATA‑TCTGCGGGGGTAttga                                                                                                                                                                                      >  1:272098/1‑236 (MQ=255)

                                                               tGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCCTCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCACCGCCCAAATATTG‑TCTGCGGGGGTATGTa                                                                                                                                                                                      >  2:584475/1‑239 (MQ=255)

                                                                    cccccTGAATATCAGCCCCACTCTC‑TTGGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTAAAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGc                                                                                                                                                                                 <  2:175716/236‑1 (MQ=255)

                                                                     caccTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTTCGGTGGTATGTAATCGCt                                                                                                                                                                                >  2:1134300/1‑239 (MQ=255)

                                                                           aaTATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGtctgtctctttta                                                                                                                                                                           >  2:1050349/1‑226 (MQ=255)

                                                                            atacCAGCCCCACGCTC‑TCTGGCTTGTTCTTCTGTCATCCCACCCCTGGCCAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTCTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAAc                                                                                                                                                                         <  2:9212/239‑1 (MQ=255)

                                                                               tCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGGTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCCGTGGTATTGAATCGCTTGTCAACGAc                                                                                                                                                                      >  2:367443/1‑239 (MQ=255)

                                                                                           tc‑tctGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCCGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAAAATCTCCCATCGCCCAAATATTT‑TCTGCGGTGGTAGTTAATCCCTTTTCAACGCAA‑ATT‑Ggccccc                                                                                                                                                         >  2:19973/1‑236 (MQ=255)

                                                                                                   cTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGGATGTAATCGCTTGTCAACGACA‑ATT‑TCCCCGCGCTCGttt                                                                                                                                                 >  2:1272104/1‑239 (MQ=255)

                                                                                                      gTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGTGGGTATGGAATCGCTTGGCAACGACA‑ATT‑TCCCCCCCCCCGttt                                                                                                                                                 >  2:24967/1‑236 (MQ=255)

                                                                                                      gTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCC‑CCGGTAAAATATCCCAACGCCCAAAAATTG‑TCTGCGGGGGCATGTAATACCTTTTCAAC‑ACACATT‑TCCCCCCGCCCGTTTaac                                                                                                                                              >  1:1225044/1‑237 (MQ=255)

                                                                                                      gTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGGGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAAAATTT‑TCTGCGGGGGTATGTAAACCCTTGTCAACCAAA‑AAT‑TCCCCGCGCCCGgtttag                                                                                                                                              >  1:540315/1‑236 (MQ=255)

                                                                                                       ttcttcTGTCATACCAACCCTGGACAGGGGCGGTGTCCTAAATACGGAATAAGGCACATTTTTCCGATCCTCAGTACTACGTTTTCCTTCACCCAGTAAATCATCACGGACAATGCGGTAATCATCCAGTGATATTAAAGTAAATTGCAACCCG‑CCGGGAACAACTCCCATCGCCCAAATATTGCTTTGCGGGGATA‑CTAACCGCTTGTACACGAAC‑AAT‑GTCCCCCGCCCGTTTAcag                                                                                                                                             >  1:1166421/1‑237 (MQ=255)

                                                                                                            ctGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAAACGCTTGTCAACAAAA‑ATT‑TCCCCGGCCCCGGTTACTGCGaaa                                                                                                                                        >  1:1027112/1‑237 (MQ=255)

                                                                                                                         ccTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCCGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAAAATCTCCCATCGCCCCAATATTG‑TCTTCGGGGGCATGTAAACGCTTGTCAACCACA‑ATTCCCCCCGCGC‑CGTTTTCTTCGTAACCGGCAtttttat                                                                                                                           >  1:250667/1‑236 (MQ=255)

                                                                                                                         ccTGGACAGGGGCGGGGTCATAAATACGGAATAAAGCACATTTTTCCGATCATCAGTACTACGGTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTTATCATCCAGTGATATGTAAGTAAATTGCATCCCC‑CCTGAAAGATTTCCCAACGCCCCAAAGTTG‑TCGGCGGTGGTGTGGCATTGCATGGCCACGGCA‑ACT‑GCACCTGGCTCGTTTATCGCGAAACCCCCATTTTCTg                                                                                                                           >  1:717916/1‑239 (MQ=255)

                                                                                                                           tggcccggggggttttCATAAATCCGGAAAAAGCCACATTTTTCCGATCATGCGTTCTTCGTTTGCCCTCACCCATTAACCCATCCCGTACAATGCGGTAATCCTCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGa                                                                                                                         <  1:559981/224‑1 (MQ=255)

                                                                                                                             gACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGGATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCCCGCCCGTTTACTGCGATACCGGCATTTTCTGGATg                                                                                                                       >  2:293049/1‑239 (MQ=255)

                                                                                                                             gACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑ACTGCGGTGGGATGTAATCCCTTGTCAACAACA‑ATT‑TCCCCGCGCCCGTTTACTGCGGTACCGGCATTTTTTGGGTg                                                                                                                       >  1:656405/1‑239 (MQ=255)

                                                                                                                                  gggCGGTGTCATAAATACGGAATAATGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCAACCAGTAACTCATCACGTACAAAGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCAGCGCCCAAATATTT‑TCTGCGGTGGGATGTAATCCCTTGTCAACAGCA‑ATT‑CCCCCCCGCTCGGTTAATGCGAAACCCGCATTTTTTTGATTTAACg                                                                                                                  >  1:906428/1‑239 (MQ=255)

                                                                                                                                  gggCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTa                                                                                                                                                                                          >  2:1117961/1‑169 (MQ=255)

                                                                                                                                  gggCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGCCA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACg                                                                                                                  >  2:605607/1‑239 (MQ=255)

                                                                                                                                  gggCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAAAATTT‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCCCCCTCGTTTACTGCGATACCGGCATTTTTTGGAGTTAACg                                                                                                                  >  1:528118/1‑239 (MQ=255)

                                                                                                                                   ggCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGCAATTAACGa                                                                                                                 >  2:380305/1‑239 (MQ=255)

                                                                                                                                   ggCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGGACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCCAATATTG‑TCTGCGGAGGTATGTAATCGCTTGGCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGCTACCGGCATTTTCTGGATGTAACGa                                                                                                                 >  2:429061/1‑239 (MQ=255)

                                                                                                                                       gtgtCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGGGATCCCGGCATTTTCTGGATGTAACGAAGCg                                                                                                             >  2:706009/1‑239 (MQ=255)

                                                                                                                                        tgtCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCcgcg                                                                                                                                                        <  2:184991/195‑1 (MQ=255)

                                                                                                                                                    ggAATAAGTCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTTCAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAACTATTG‑TCTTCGGTGGTATGGAAACCCTTGTCACCGACA‑ATT‑GCCCCCCCCTCGTTTCCTCCAATCCCGACCTTTTTGGAATGTaaacagccggagtcgggttaac                                                                                                >  1:303551/1‑219 (MQ=255)

                                                                                                                                                    ggAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGCCA‑ATT‑GCCCCGCGCTCGTTTACTGCGTTACCGGCATTTTCTGGATGGAACGACACGGTAACCGGTGgac                                                                                                >  1:360070/1‑239 (MQ=255)

                                                                                                                                                      aaTAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCTGTGATATGTAAGTAAATTGCCGCCCG‑ACGGTAACATCTCCCATCGCCCAACTATTG‑TCTGCGTGGGAATGTAACCCCTTGTCAACGCCC‑ATT‑GCCCCGCGCTCGTTTCATACGAGACCGGCATTTTCTGGAGGAAACGAACCGGTACCCAGTTgacaa                                                                                              >  1:252856/1‑237 (MQ=255)

                                                                                                                                                      aaTAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATTTAACGAAGCGGTAGCCGGTTgacg                                                                                               >  2:591421/1‑238 (MQ=255)

                                                                                                                                                          cgggacttttttCCTATCACTAGTTCCAGGTTTACCCTCCCCCGATAACCCAACCCTTAACAGGGGGAAATCACCCAGTGATATGTAGGGAAAGTGCAGCCCG‑CCGGTAACATCGCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                          <  1:1044626/232‑1 (MQ=255)

                                                                                                                                                          aggccaaTTTTTCCGATCATCAGTATTCCGTTTGCCTTCCCCCAGTAACTCATCCCGTACAAGGCGGTAATCATCCAGTGATAGTTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                          <  2:1225044/233‑1 (MQ=255)

                                                                                                                                                          aGGCACATTTTTCCGATCATCAGTCCTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGAAATGTAAGTAAATGGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                          <  2:1027112/239‑1 (MQ=255)

                                                                                                                                                          aGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCCCCCAGTAACTCATCACGTACAAGGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                          <  2:296264/239‑1 (MQ=255)

                                                                                                                                                          aGGCACATTTTTCCGATCACCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCgg                                                                                          <  2:528118/239‑1 (MQ=255)

                                                                                                                                                                aTTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGGAGCCGGTGGAGGCCCGGaggata                                                                                    >  1:1246963/1‑234 (MQ=255)

                                                                                                                                                                  ttttCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTAtt                                                                                  >  2:189020/1‑239 (MQ=255)

                                                                                                                                                                   ttaccGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTa                                                                                 <  2:1321149/236‑1 (MQ=255)

                                                                                                                                                                                  tcgccGTTTGCCTTCCCCCAGAAACCCATCCCGTACAATGGGTTAATCTCCCAGTGATATGTAAGTAAATTGCCGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCg                                                                <  2:1009811/235‑1 (MQ=255)

                                                                                                                                                                                      cGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACt                                                                                                                                              >  2:863859/1‑159 (MQ=255)

                                                                                                                                                                                      cGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGCCGACCGGAAGCTATTAACAGGTC‑A‑TCCACCCCCAg                                                            >  1:1009811/1‑239 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGCTATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGAGATAACGGCATTTTCTGGTGTTAACGACGCGGGTGCCGGTTGCCGCACGGAACTTTTTCACAGTGA‑C‑TGCACCGCCCGCTggg                                                       >  1:469854/1‑239 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGA‑A‑TCCACCGCCAGTTggg                                                       >  2:1108519/1‑239 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGGGC‑A‑TCCACCGCCAGTTggg                                                       >  2:277584/1‑239 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTGGACGCCCGGAAGCTATTAAAAGGGA‑A‑TCCACCGCCAGTTggg                                                       >  2:317654/1‑239 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAAAGAAGCGGTAGCTGGTTGACGACCGGAAGCTCTTAACAGCGC‑A‑TCCACCGCCAGTTgg                                                        >  2:788776/1‑238 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGGTTACTGCGTTAACGGCATTTGCTGGAGGTACCGAAGCGGAAGCGGGTTGAAGTCCGGAGACTTTTAAAAGGGA‑A‑TACACAGccgactggg                                                       >  1:460814/1‑232 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑ACCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGTTGTAACGAAGCGGGAGCCGGTTGCCAACCGGAAACCATTAACA‑TGCAA‑TCCACACCCAATTggg                                                       >  1:627920/1‑239 (MQ=255)

                                                                                                                                                                                           gCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGGATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATTACGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGAGGCCCGGAAGCTATTAAAAGGGC‑A‑TCATCCGCCAATTggg                                                       >  2:698181/1‑239 (MQ=255)

                                                                                                                                                                                                cACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACGGCCAGTTGGGCgcg                                                   >  2:799025/1‑236 (MQ=255)

                                                                                                                                                                                                      ttactccatccCGTAAAATGCGGTAATCACCCAGGGATATGTAAGTAATTGGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTa                                            <  2:540315/233‑1 (MQ=255)

                                                                                                                                                                                                             atcaCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAATTATTG‑TCTGCg                                                                                                                                                                                                >  1:130782/1‑88 (MQ=255)

                                                                                                                                                                                                             atcaCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTg                                                                                                                           >  2:12484/1‑155 (MQ=255)

                                                                                                                                                                                                             atcaCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGaa                                                                                        <  2:1117020/190‑1 (MQ=255)

                                                                                                                                                                                                             atcaCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGaa                                                                                        >  1:1117020/1‑190 (MQ=255)

                                                                                                                                                                                                             atcaCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTACAAGTGC‑A‑TCCACCGCCAGGTGGGCGTGCTCGCTAtgcactt                                     >  2:1032932/1‑239 (MQ=255)

                                                                                                                                                                                                             atcaCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGAGTTAACGCAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGGGCCCGCtggggaatt                                     >  1:625490/1‑231 (MQ=255)

                                                                                                                                                                                                             atcaCGTACAATGCGGAAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCg                                                                                                                                                                                                <  2:130782/88‑1 (MQ=255)

                                                                                                                                                                                                               caCGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCCCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGCCCGGAAGCATTTAACAGGGC‑A‑TCCACCCCCGATTgggggggcccccaagccccttg                                    >  1:148828/1‑219 (MQ=255)

                                                                                                                                                                                                                       aTGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTGTCACTTGAtt                           >  2:815466/1‑239 (MQ=255)

                                                                                                                                                                                                                       aTGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGGTGGGCGTGCTCGCTATGCACTTGCACTTGAtt                           >  2:42358/1‑239 (MQ=255)

                                                                                                                                                                                                                       aTGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGATGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGAtt                           >  2:1196565/1‑239 (MQ=255)

                                                                                                                                                                                                                        tGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACATTGA‑C‑TCCACGCCCATTTGGGGTGGCTCGCTGGACCCTGACACTTGAttt                          >  1:593744/1‑239 (MQ=255)

                                                                                                                                                                                                                          cGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCCCTTGCACTTGGTTTT‑c                       >  2:771346/1‑239 (MQ=255)

                                                                                                                                                                                                                            gTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTACCGAAGCGGTAGCCGGTTAACGCCAGGAAGCTATT‑ACAATGT‑ACACCCCCGCCAGTGGGGCGGGCCCgacgttacctgcccctggatttt                         >  1:465581/1‑214 (MQ=255)

                                                                                                                                                                                                                            gTAATCATCCAGTGATATGAAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTAt                                                                                                                                                                                         >  2:871495/1‑80 (MQ=255)

                                                                                                                                                                                                                            gTAATCATCCAGTGATATGAAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTAt                                                                                                                                                                                         <  1:871495/80‑1 (MQ=255)

                                                                                                                                                                                                                             tAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTT‑CATg                    >  2:454130/1‑239 (MQ=255)

                                                                                                                                                                                                                               atcatcCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCCGTGGGGCGTGCTCGCTATGCACTTGCACTTGATTTT‑CATggg                  >  2:468007/1‑238 (MQ=255)

                                                                                                                                                                                                                                 tacccAGTGATATGAAAGAAAATGGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTT‑CATGGTGa                <  2:656405/236‑1 (MQ=255)

                                                                                                                                                                                                                                  atcCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCcgcg                                                                                                                                                        <  2:1066362/105‑1 (MQ=255)

                                                                                                                                                                                                                                  atcCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCcgcg                                                                                                                                                        >  1:1066362/1‑105 (MQ=255)

                                                                                                                                                                                                                                  atcCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGATGGCATGTAATCGCTTGTCAACGACA‑ATT‑GCACCGCGCTCGTTTACTGCGCTACCGGAATTTCCTGGATGTAACGAAGCGGTAGCCGGTTTACGACCGGAAGCTATTAAAACGAC‑A‑CCCACCGCCAGTTGGGCGGGCACGCTATGC‑CATTCCCCTGATTTTCCATGGTgca               >  1:850455/1‑237 (MQ=255)

                                                                                                                                                                                                                                      tgtgttttGTAAGTAAAGTGCACCCCG‑CCGGTAACATCCCCCATCGCCCAAATTTTG‑TCTGCGGTGGTATGTAATCGGTTGTCAACGAAA‑ATT‑GCCCCGGGCTCGTTTACTGCGATCCCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTT‑CATGGTGACTGAt           <  2:810106/232‑1 (MQ=255)

                                                                                                                                                                                                                                       gTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGCTACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCACCCGTTCGGCTGGCTGGCTATGCCCTTTCCATTGATTTT‑TCTGGGGAcgaaat          >  1:875779/1‑234 (MQ=255)

                                                                                                                                                                                                                                          atatGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCACATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTCCTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAACCGGCTGCGGAACGGCAGCTATTAACAGTGC‑A‑TCCGCCGCCCGTTGGGGGGAGTCGCGATCGCCTTGAACTTGATTTT‑AATTGTGACTGAtttg        >  1:867691/1‑236 (MQ=255)

                                                                                                                                                                                                                                             tGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCCCCGCCAGTTGGCCGGGCTCGCTATGCCCTTTCACTTGATTTT‑CATGGGGCCTGATTCGCTcc    >  1:161227/1‑239 (MQ=255)

                                                                                                                                                                                                                                               taagtaaATTCCACCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTATCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTT‑CATGGTGACTGATTCGCTCcac  <  2:1103758/239‑1 (MQ=255)

CGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTC‑TCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGTCATAAATACGGAATAAGGCACATTTTTCCGATCATCAGTACTACGTTTGCCTTCACCCAGTAACTCATCACGTACAATGCGGTAATCATCCAGTGATATGTAAGTAAATTGCAGCCCG‑CCGGTAACATCTCCCATCGCCCAAATATTG‑TCTGCGGTGGTATGTAATCGCTTGTCAACGACA‑ATT‑GCCCCGCGCTCGTTTACTGCGATACCGGCATTTTCTGGATGTAACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGC‑A‑TCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTT‑CATGGTGACTGATTCGCTCCAC  >  NC_000913/318862‑319338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`