Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,460,785 | T→C | 17.4% | F324S (TTC→TCC) | gspF → | general secretory pathway component, cryptic |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,460,785 | 0 | T | C | 17.4% | 182.0 / 6.3 | 74 | F324S (TTC→TCC) | gspF | general secretory pathway component, cryptic |
Reads supporting (aligned to +/- strand): ref base T (52/7); new base C (2/13); total (54/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.20e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGA > NC_000913/3460554‑3461014 | ccATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑Cccgccg > 2:355308/1‑239 (MQ=255) tGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TACCAGCATTCATGTTTCGCTGGAACAAACCGC‑AAT‑TTT‑Cccgccgcgg > 2:342983/1‑236 (MQ=255) tGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCACCCGATAGCCTCCACAACCTGGCAAT‑T‑CGCCAGCGTCTGGCCAATTCGGCCGAGAACGCTAGCCCGGG‑GAAACCAATTCATCTTTCGCTGGAACAAAACCC‑AAT‑TTC‑ACCGCCgaag > 1:527366/1‑237 (MQ=255) gctgcGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGAATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGaggatg > 2:660457/1‑238 (MQ=255) cgTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCGGCGTCTGGCAAATTCGGCAGAGAACGTTCGCCAGGG‑TAAAAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTCCa > 1:1031914/1‑239 (MQ=255) ttGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGACCGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGAGGATGCTCTACAtg > 2:943317/1‑239 (MQ=255) aTCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTACATGGTGGcc > 2:1301810/1‑237 (MQ=255) gCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCACCAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATCCGGCAGAAACCGATCGCCAGGG‑GAACAGCATTCCTCTTTCGCTTGAAAACACAAC‑AAT‑TTT‑CCCGCCGATAATGCCCTAACTGGTGGGCTCAGGCGaaa > 1:812817/1‑239 (MQ=255) gCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATCCTCTCCATGTTGGCCTCTGGCGaaa > 2:95756/1‑239 (MQ=255) tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa > 2:641412/1‑239 (MQ=255) tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa > 2:424369/1‑239 (MQ=255) tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCCC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa > 2:577786/1‑239 (MQ=255) gcACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGTAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑ACT‑TTT‑ACCGCCGATGATGCCTTACCTGGTGGCCTCGGGCGAAAAACCCGGGccacacggccaatt > 1:793154/1‑226 (MQ=255) gcACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGCAAAAAGCGGGCAGCTCGG‑CACAtt > 2:775069/1‑239 (MQ=255) aCGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGAAGCTCGG‑CACATTaa > 2:285093/1‑239 (MQ=255) ctcCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCTAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCGGGAACAAACCGC‑AAT‑TTT‑CCCGCCACTGTGGCCCTCCTTGTGGGCCTCTGGCGAAAAAAACGGCCTACACGG‑CCCCTTAATGGTccg > 1:67602/1‑237 (MQ=255) cACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CCCATTAATGGTCAGAGcc > 2:645205/1‑238 (MQ=255) ttttttatttttttCCAACCCAGCGGTCCCCCTGGGGGGGGTGGGAT‑AATTTCGCCCCCCGAACCCCCAACCACCCCAAAATCT‑CGCCGGGGTAGGGAAAAGGCGGAAGAGAACGTTCGCCGGGG‑AAACAGCATTCTTCTTTCGGTGGAACAAACCGA‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGc < 1:395814/229‑1 (MQ=255) ttAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAGCCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGCTGCTCTACATGGTGGCCTCCGGCGAAAAAAGCGGGCCGCTCGG‑CACATTAATGGTCAAAGcccccaa > 1:482208/1‑234 (MQ=255) tAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGCTGAACCTCTACATTGTGGCATCTGGCGAAAAAAACGGGCACCTCGGCCACA‑TAATGTGCAGAGCCGgagaa > 1:1200530/1‑238 (MQ=255) tAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAAAAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGTGGGCCACTGGGGAAAAAAGCCGGAAGCTGGG‑CACATTAAAGGTCAAACCCGCAGaa > 1:946827/1‑238 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGCAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACCGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTCCATGGTGGCCTCCGGCGAAAAAAGCGGGCAGCTCCG‑GCCCTTAATGGTCGGAACCGCAGACAAACAAga > 1:986769/1‑239 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACCGCATTCATCTTTCGCTGGAACACACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTACATGATGGCCTCTGGCGACAAAAACGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAggg > 2:948369/1‑238 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑ACT‑TTT‑CCCGCCGATGATGCTCGACATGTTGGCCTCTGGCGAAAAACGCGTGCAGCTGGG‑CACATTAATGGTCAAAGCCGACGAAAACCAgag > 1:1007801/1‑237 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTa > 2:1128013/1‑170 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGTTAACCAGga > 2:79574/1‑239 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGCCAGAGCCGCCGTTAACCAgta > 2:341038/1‑237 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAACGGGCAGCTCGG‑CACATTCATGGTCAGAGCCCGAGGTAACCAGga > 2:1223302/1‑239 (MQ=255) ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGAAGAGAACGTTCGCCAGGG‑TAACAGCATTCTTCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGTCTTACATGGTGGCCTCGGGCGCACAAAGCGGGGAGCTCGG‑CCCAAGAAAGGACCGAACCGCacaaaaacaaga > 1:731376/1‑228 (MQ=255) tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACaaaa > 2:395602/1‑238 (MQ=255) tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGAAACTCCAACaaaa > 2:1324562/1‑238 (MQ=255) tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGACGATGCCCAACATGGTGGCCTCTGCCGAAAAAAGCGGGCAGCCCGC‑GACCTTAAAGGTCAAAGCCACAGAAAAACAAGAGAAACCACAaaaaaaa > 1:646448/1‑238 (MQ=255) tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGACAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTACATGGTGGCCTCTGGCGAACAAAGCGGGCAGGTCGG‑CACATTTATGGTCCGGCCCGCCAATAAcacgggaaacccccaaaaaaaa > 1:990645/1‑218 (MQ=255) gatgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGGGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑ACT‑TTT‑CCCGCCGATGATGCTCTCAATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑AACATTCATGGTCAGAGCCCCCGATAACCAGGAACAACACCAACAAAAACGAAACGcgta > 1:492315/1‑236 (MQ=255) gatgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCtt > 2:689193/1‑239 (MQ=255) tgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGAAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGACGCAAAAATCGGCCAGCTCGG‑CACATAAATGGTCAGAGCCGCAGAAACCCAGGAGACCCTCCAACAAAAACGGACCGccacaa > 1:1311749/1‑235 (MQ=255) gtagAATTTCTCCCCCAAAATCCACAAC‑ACCCCAAAAT‑TCCCCCAGCTTCTGGAAAGGGCGGCAAAAAACTTTCCCCAGGG‑AAAGGGCATTCATTTTTCGCGGGAACAACCCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg < 2:986769/235‑1 (MQ=255) gatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg > 2:1209913/1‑238 (MQ=255) gaggAATTTGCCCACCAAAAGCCTAACAAACCTCGAAAT‑T‑CGCCGGCTTCGGGCAAAGGCGGAAAAGACCGTCCGCCGGGG‑TAACAGCATTCATCTTTCGGTGGAAAAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg < 2:485123/235‑1 (MQ=255) tgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGCTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGct > 2:299985/1‑238 (MQ=255) tCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAACAAAGCAGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCCGATAACCAGGAGACACTCCAACAAAATCGGATTGCCTTAACGCTCTCCATCtt > 2:31075/1‑239 (MQ=255) tCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGAAACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCt > 2:242060/1‑238 (MQ=255) aGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAACAGCGGGCAGCTCGG‑CACCTTAATGGTCAGAGCCGCAGATAACCAGGGGACACCCCAACAAAATCGGAACGCCTTAACGcctgtctcttaaaaccatcc > 1:1208798/1‑220 (MQ=255) cccccaacacCCTGAAAAT‑C‑CGCCAGGGTGCGACAAAGGCGGAAGAGAACGGTCGCCAGGG‑TAACAGCATTCATCTTGCGCTGAAACAACCCGC‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa < 2:1031914/235‑1 (MQ=255) tCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCACCAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa > 2:698674/1‑236 (MQ=255) caaaattgcGCCAGCCTCTGGAAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACACGATTCTTCTTGCGCGGAAACAACCCGA‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACg < 2:588968/231‑1 (MQ=255) ggagatccgcgagggtggggaaaaCGCGGAAGAGAGCGTGCGCAAGGG‑AAAACGTTTCCCTCTGTCGCGGGAACAACCCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGa < 2:204618/218‑1 (MQ=255) caatatccgcgggcttggggaaaaTGGGGCAGAGAACGTTCCACGGGT‑AAACAGCATTCATCTTTCGCGGAAAAAACCCGA‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGa < 2:337539/218‑1 (MQ=255) cGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CTCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCTTAATTACGATGGCACTGATCg > 2:1027500/1‑238 (MQ=255) gTCGGGAAAACGGGGCAGAGCACTTCCGCCGGGG‑AAACCGATTCCATTTTTCGC‑GGAACAAACCCG‑AAT‑TTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc < 2:812817/239‑1 (MQ=255) tgtggaaaggggcgaagagacgtttCGCCGGGT‑AAACAGTATTCATTTTCGGGTGAAAAAACCCGA‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc < 2:482208/215‑1 (MQ=255) tgtggaaacgggggCAAAGACCGTCCGCGGGGG‑TACCGGATTTTATTTTGCGCGGGAACAACCCTA‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGACCGTc < 2:101340/226‑1 (MQ=255) gggaaacggCGGCAGAAGCCGTCCCCAAGGG‑TAACAGCTTGCTTTGTGCGCGGAAACAACCCGA‑AAT‑GTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt < 2:990645/230‑1 (MQ=255) tGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATAGCCTTAACGCTCTCCCTCTTCGAGCCAGCCCTCATTATTACGATGGCACTGATCGTCCTGTTTAtt > 2:1034872/1‑238 (MQ=255) acgtccccAGGGATAACCGCATTCATTTCTCGGT‑GAAAAAACCCC‑AATGTTC‑CCCGCCGATGATGCCCTACATGGGTGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTa < 2:65225/233‑1 (MQ=255) tCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtgtttgtctcttttcc > 2:937281/1‑225 (MQ=255) tCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACAATCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGCTACTcc > 2:185478/1‑239 (MQ=255) gCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCa > 2:1265384/1‑238 (MQ=255) ccAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAAACGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGCGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGCGCCAGCCATCATTATTACGATGGCACTGATCGTCCTGTTTATTCTCCTGTCGGCACTcaca > 1:284804/1‑235 (MQ=255) acatatttctttttgcgcggcaaCAAACCGC‑ATT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTcttctt < 2:665154/218‑1 (MQ=255) cAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCttcttc > 2:394918/1‑239 (MQ=255) tttCATCTTCCG‑GGGAAAAAACCCCAAAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACt < 2:646448/238‑1 (MQ=255) ttCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCCGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAAAGCTCTCCATCTTCGAGCCACCACTCATTATTACGTAGGGCTTGATCGTCCTGTTTTTTTTCGTGCCCGAATCCCAACACCTTTTTCAACtg > 1:335668/1‑238 (MQ=255) ttCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGCTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCCCTGACCGTCCTGTTTCTTGCCGTGTCGGTACTCCAACCTCTTTTTCAACtt > 1:1052138/1‑239 (MQ=255) ttCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCG‑AAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTa > 2:1124818/1‑239 (MQ=255) tCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCCCTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTa > 2:177853/1‑239 (MQ=255) gtgaaaaaacccGC‑AAT‑TTC‑CCCGCCGATGATGCTTTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGat < 2:793154/229‑1 (MQ=255) cTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAATAAAGCGGGCAGCTCGA‑CACATTAATGGGCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGCTGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTTTTCAAATTAACTCCATGat > 2:732434/1‑239 (MQ=255) ggaaaaaaaCCCA‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGat < 2:479400/232‑1 (MQ=255) aacacccactaT‑TCT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CAAATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGattaatt < 1:395602/229‑1 (MQ=255) aaaCCGT‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCG‑AAAAAGCGGGCAGCTCGG‑CACATTAATGGGCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg < 2:478032/239‑1 (MQ=255) cctccataT‑TTC‑CCCGCCGATGAGGCTCTACATGGGGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGGCAGGGCCGCAGATAACCAGGGGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg < 1:904617/232‑1 (MQ=255) accccattt‑ttc‑CCCGCCGATGATGCTCTACCTGGTGGCCTCTGGCGAAAAAAGGGGGCAGCTCGG‑CAAATTAATGGTCAGAGCCGCAGTTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg < 1:79574/231‑1 (MQ=255) acccagaaT‑TTC‑CCCGCCGATGATGCTCTTACTGGGGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CAGATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg < 2:284804/233‑1 (MQ=255) ccacatt‑ttc‑CCCGCCGATGATGCTCTAAATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGa < 2:666915/233‑1 (MQ=255) aaT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtg > 2:59618/1‑182 (MQ=255) | CCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGA > NC_000913/3460554‑3461014 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |