Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,460,785 T→C 17.4% F324S (TTC→TCC)  gspF → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,460,7850TC17.4% 182.0 / 6.3 74F324S (TTC→TCC) gspFgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base T (52/7);  new base C (2/13);  total (54/20)
Fisher's exact test for biased strand distribution p-value = 6.20e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGA  >  NC_000913/3460554‑3461014
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
ccATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCccgccg                                                                                                                                                                                                                                 >  2:355308/1‑239 (MQ=255)
   tGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTACCAGCATTCATGTTTCGCTGGAACAAACCGCAATTTTCccgccgcgg                                                                                                                                                                                                                              >  2:342983/1‑236 (MQ=255)
   tGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCACCCGATAGCCTCCACAACCTGGCAATTCGCCAGCGTCTGGCCAATTCGGCCGAGAACGCTAGCCCGGGGAAACCAATTCATCTTTCGCTGGAACAAAACCCAATTTCACCGCCgaag                                                                                                                                                                                                                              >  1:527366/1‑237 (MQ=255)
       gctgcGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGAATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGaggatg                                                                                                                                                                                                                           >  2:660457/1‑238 (MQ=255)
             cgTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCGGCGTCTGGCAAATTCGGCAGAGAACGTTCGCCAGGGTAAAAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCCCTCCa                                                                                                                                                                                                                    >  1:1031914/1‑239 (MQ=255)
               ttGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGACCGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGAGGATGCTCTACAtg                                                                                                                                                                                                                  >  2:943317/1‑239 (MQ=255)
                       aTCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCCCTACATGGTGGcc                                                                                                                                                                                                            >  2:1301810/1‑237 (MQ=255)
                               gCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCACCAACCTCGAAATTCGCCAGCGTCTGGCAAATCCGGCAGAAACCGATCGCCAGGGGAACAGCATTCCTCTTTCGCTTGAAAACACAACAATTTTCCCGCCGATAATGCCCTAACTGGTGGGCTCAGGCGaaa                                                                                                                                                                                                  >  1:812817/1‑239 (MQ=255)
                               gCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATCCTCTCCATGTTGGCCTCTGGCGaaa                                                                                                                                                                                                  >  2:95756/1‑239 (MQ=255)
                                 tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa                                                                                                                                                                                                >  2:641412/1‑239 (MQ=255)
                                 tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa                                                                                                                                                                                                >  2:424369/1‑239 (MQ=255)
                                 tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCCCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa                                                                                                                                                                                                >  2:577786/1‑239 (MQ=255)
                                                     gcACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGTAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCACTTTTACCGCCGATGATGCCTTACCTGGTGGCCTCGGGCGAAAAACCCGGGccacacggccaatt                                                                                                                                                                            >  1:793154/1‑226 (MQ=255)
                                                     gcACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGCAAAAAGCGGGCAGCTCGGCACAtt                                                                                                                                                                           >  2:775069/1‑239 (MQ=255)
                                                       aCGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGAAGCTCGGCACATTaa                                                                                                                                                                         >  2:285093/1‑239 (MQ=255)
                                                              ctcCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCTAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCGGGAACAAACCGCAATTTTCCCGCCACTGTGGCCCTCCTTGTGGGCCTCTGGCGAAAAAAACGGCCTACACGGCCCCTTAATGGTccg                                                                                                                                                                  >  1:67602/1‑237 (MQ=255)
                                                                   cACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCCCATTAATGGTCAGAGcc                                                                                                                                                              >  2:645205/1‑238 (MQ=255)
                                                                    ttttttatttttttCCAACCCAGCGGTCCCCCTGGGGGGGGTGGGATAATTTCGCCCCCCGAACCCCCAACCACCCCAAAATCTCGCCGGGGTAGGGAAAAGGCGGAAGAGAACGTTCGCCGGGGAAACAGCATTCTTCTTTCGGTGGAACAAACCGAAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGc                                                                                                                                                            <  1:395814/229‑1 (MQ=255)
                                                                       ttAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAGCCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGCTGCTCTACATGGTGGCCTCCGGCGAAAAAAGCGGGCCGCTCGGCACATTAATGGTCAAAGcccccaa                                                                                                                                                         >  1:482208/1‑234 (MQ=255)
                                                                        tAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGCTGAACCTCTACATTGTGGCATCTGGCGAAAAAAACGGGCACCTCGGCCACATAATGTGCAGAGCCGgagaa                                                                                                                                                        >  1:1200530/1‑238 (MQ=255)
                                                                        tAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAAAAAACCGCAATTTTCCCGCCGATGATGCTCTACATGTGGGCCACTGGGGAAAAAAGCCGGAAGCTGGGCACATTAAAGGTCAAACCCGCAGaa                                                                                                                                                        >  1:946827/1‑238 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGCAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACCGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCCCTCCATGGTGGCCTCCGGCGAAAAAAGCGGGCAGCTCCGGCCCTTAATGGTCGGAACCGCAGACAAACAAga                                                                                                                                                >  1:986769/1‑239 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACCGCATTCATCTTTCGCTGGAACACACCGCAATTTTCCCGCCGATGATGCCCTACATGATGGCCTCTGGCGACAAAAACGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAggg                                                                                                                                                >  2:948369/1‑238 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCACTTTTCCCGCCGATGATGCTCGACATGTTGGCCTCTGGCGAAAAACGCGTGCAGCTGGGCACATTAATGGTCAAAGCCGACGAAAACCAgag                                                                                                                                                >  1:1007801/1‑237 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTa                                                                                                                                                                                                                      >  2:1128013/1‑170 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGTTAACCAGga                                                                                                                                                >  2:79574/1‑239 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGCCAGAGCCGCCGTTAACCAgta                                                                                                                                                >  2:341038/1‑237 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAACGGGCAGCTCGGCACATTCATGGTCAGAGCCCGAGGTAACCAGga                                                                                                                                                >  2:1223302/1‑239 (MQ=255)
                                                                                ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGAAGAGAACGTTCGCCAGGGTAACAGCATTCTTCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGTCTTACATGGTGGCCTCGGGCGCACAAAGCGGGGAGCTCGGCCCAAGAAAGGACCGAACCGCacaaaaacaaga                                                                                                                                                >  1:731376/1‑228 (MQ=255)
                                                                                                tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACaaaa                                                                                                                                 >  2:395602/1‑238 (MQ=255)
                                                                                                tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGAAACTCCAACaaaa                                                                                                                                 >  2:1324562/1‑238 (MQ=255)
                                                                                                tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGACGATGCCCAACATGGTGGCCTCTGCCGAAAAAAGCGGGCAGCCCGCGACCTTAAAGGTCAAAGCCACAGAAAAACAAGAGAAACCACAaaaaaaa                                                                                                                                >  1:646448/1‑238 (MQ=255)
                                                                                                tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGACAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCCCTACATGGTGGCCTCTGGCGAACAAAGCGGGCAGGTCGGCACATTTATGGTCCGGCCCGCCAATAAcacgggaaacccccaaaaaaaa                                                                                                                                >  1:990645/1‑218 (MQ=255)
                                                                                                           gatgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGGGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCACTTTTCCCGCCGATGATGCTCTCAATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGAACATTCATGGTCAGAGCCCCCGATAACCAGGAACAACACCAACAAAAACGAAACGcgta                                                                                                                     >  1:492315/1‑236 (MQ=255)
                                                                                                           gatgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCtt                                                                                                                     >  2:689193/1‑239 (MQ=255)
                                                                                                             tgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGAAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGACGCAAAAATCGGCCAGCTCGGCACATAAATGGTCAGAGCCGCAGAAACCCAGGAGACCCTCCAACAAAAACGGACCGccacaa                                                                                                                   >  1:1311749/1‑235 (MQ=255)
                                                                                                                gtagAATTTCTCCCCCAAAATCCACAACACCCCAAAATTCCCCCAGCTTCTGGAAAGGGCGGCAAAAAACTTTCCCCAGGGAAAGGGCATTCATTTTTCGCGGGAACAACCCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg                                                                                                                 <  2:986769/235‑1 (MQ=255)
                                                                                                                gatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg                                                                                                                 >  2:1209913/1‑238 (MQ=255)
                                                                                                                gaggAATTTGCCCACCAAAAGCCTAACAAACCTCGAAATTCGCCGGCTTCGGGCAAAGGCGGAAAAGACCGTCCGCCGGGGTAACAGCATTCATCTTTCGGTGGAAAAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg                                                                                                                 <  2:485123/235‑1 (MQ=255)
                                                                                                                  tgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGCTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGct                                                                                                               >  2:299985/1‑238 (MQ=255)
                                                                                                                          tCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAACAAAGCAGGCAGCTCGGCACATTAATGGTCAGAGCCGCCGATAACCAGGAGACACTCCAACAAAATCGGATTGCCTTAACGCTCTCCATCtt                                                                                                      >  2:31075/1‑239 (MQ=255)
                                                                                                                          tCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGAAACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCt                                                                                                       >  2:242060/1‑238 (MQ=255)
                                                                                                                                   aGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAACAGCGGGCAGCTCGGCACCTTAATGGTCAGAGCCGCAGATAACCAGGGGACACCCCAACAAAATCGGAACGCCTTAACGcctgtctcttaaaaccatcc                                                                                             >  1:1208798/1‑220 (MQ=255)
                                                                                                                                    cccccaacacCCTGAAAATCCGCCAGGGTGCGACAAAGGCGGAAGAGAACGGTCGCCAGGGTAACAGCATTCATCTTGCGCTGAAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                                                            <  2:1031914/235‑1 (MQ=255)
                                                                                                                                       tCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCACCAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                                                            >  2:698674/1‑236 (MQ=255)
                                                                                                                                                  caaaattgcGCCAGCCTCTGGAAAATGCGGCAGAGAACGTTCGCCAGGGTAACACGATTCTTCTTGCGCGGAAACAACCCGAAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACg                                                                                <  2:588968/231‑1 (MQ=255)
                                                                                                                                                   ggagatccgcgagggtggggaaaaCGCGGAAGAGAGCGTGCGCAAGGGAAAACGTTTCCCTCTGTCGCGGGAACAACCCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGa                                                                               <  2:204618/218‑1 (MQ=255)
                                                                                                                                                   caatatccgcgggcttggggaaaaTGGGGCAGAGAACGTTCCACGGGTAAACAGCATTCATCTTTCGCGGAAAAAACCCGAAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGa                                                                               <  2:337539/218‑1 (MQ=255)
                                                                                                                                                                cGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCTCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCTTAATTACGATGGCACTGATCg                                                                   >  2:1027500/1‑238 (MQ=255)
                                                                                                                                                                 gTCGGGAAAACGGGGCAGAGCACTTCCGCCGGGGAAACCGATTCCATTTTTCGCGGAACAAACCCGAATTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                                                                 <  2:812817/239‑1 (MQ=255)
                                                                                                                                                                  tgtggaaaggggcgaagagacgtttCGCCGGGTAAACAGTATTCATTTTCGGGTGAAAAAACCCGAAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                                                                 <  2:482208/215‑1 (MQ=255)
                                                                                                                                                                  tgtggaaacgggggCAAAGACCGTCCGCGGGGGTACCGGATTTTATTTTGCGCGGGAACAACCCTAAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGACCGTc                                                                 <  2:101340/226‑1 (MQ=255)
                                                                                                                                                                    gggaaacggCGGCAGAAGCCGTCCCCAAGGGTAACAGCTTGCTTTGTGCGCGGAAACAACCCGAAATGTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt                                                               <  2:990645/230‑1 (MQ=255)
                                                                                                                                                                           tGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATAGCCTTAACGCTCTCCCTCTTCGAGCCAGCCCTCATTATTACGATGGCACTGATCGTCCTGTTTAtt                                                        >  2:1034872/1‑238 (MQ=255)
                                                                                                                                                                                       acgtccccAGGGATAACCGCATTCATTTCTCGGTGAAAAAACCCCAATGTTCCCCGCCGATGATGCCCTACATGGGTGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTa                                            <  2:65225/233‑1 (MQ=255)
                                                                                                                                                                                          tCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtgtttgtctcttttcc                                         >  2:937281/1‑225 (MQ=255)
                                                                                                                                                                                          tCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACAATCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGCTACTcc                                        >  2:185478/1‑239 (MQ=255)
                                                                                                                                                                                            gCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCa                                       >  2:1265384/1‑238 (MQ=255)
                                                                                                                                                                                             ccAGGGTAACAGCATTCATCTTTCGCTGGAACAAAACGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGCGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGCGCCAGCCATCATTATTACGATGGCACTGATCGTCCTGTTTATTCTCCTGTCGGCACTcaca                                      >  1:284804/1‑235 (MQ=255)
                                                                                                                                                                                                      acatatttctttttgcgcggcaaCAAACCGCATTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTcttctt                             <  2:665154/218‑1 (MQ=255)
                                                                                                                                                                                                       cAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCttcttc                            >  2:394918/1‑239 (MQ=255)
                                                                                                                                                                                                           tttCATCTTCCGGGGAAAAAACCCCAAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACt                        <  2:646448/238‑1 (MQ=255)
                                                                                                                                                                                                            ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCCGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAAAGCTCTCCATCTTCGAGCCACCACTCATTATTACGTAGGGCTTGATCGTCCTGTTTTTTTTCGTGCCCGAATCCCAACACCTTTTTCAACtg                       >  1:335668/1‑238 (MQ=255)
                                                                                                                                                                                                            ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGCTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCCCTGACCGTCCTGTTTCTTGCCGTGTCGGTACTCCAACCTCTTTTTCAACtt                       >  1:1052138/1‑239 (MQ=255)
                                                                                                                                                                                                            ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTa                      >  2:1124818/1‑239 (MQ=255)
                                                                                                                                                                                                             tCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCCCTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTa                      >  2:177853/1‑239 (MQ=255)
                                                                                                                                                                                                                       gtgaaaaaacccGCAATTTCCCCGCCGATGATGCTTTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGat            <  2:793154/229‑1 (MQ=255)
                                                                                                                                                                                                                       cTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAATAAAGCGGGCAGCTCGACACATTAATGGGCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGCTGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTTTTCAAATTAACTCCATGat            >  2:732434/1‑239 (MQ=255)
                                                                                                                                                                                                                        ggaaaaaaaCCCAAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGat            <  2:479400/232‑1 (MQ=255)
                                                                                                                                                                                                                             aacacccactaTTCTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCAAATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGattaatt       <  1:395602/229‑1 (MQ=255)
                                                                                                                                                                                                                              aaaCCGTAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAGCGGGCAGCTCGGCACATTAATGGGCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  2:478032/239‑1 (MQ=255)
                                                                                                                                                                                                                                cctccataTTTCCCCGCCGATGAGGCTCTACATGGGGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGGCAGGGCCGCAGATAACCAGGGGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  1:904617/232‑1 (MQ=255)
                                                                                                                                                                                                                                accccatttttcCCCGCCGATGATGCTCTACCTGGTGGCCTCTGGCGAAAAAAGGGGGCAGCTCGGCAAATTAATGGTCAGAGCCGCAGTTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  1:79574/231‑1 (MQ=255)
                                                                                                                                                                                                                                acccagaaTTTCCCCGCCGATGATGCTCTTACTGGGGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCAGATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  2:284804/233‑1 (MQ=255)
                                                                                                                                                                                                                                  ccacattttcCCCGCCGATGATGCTCTAAATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGa  <  2:666915/233‑1 (MQ=255)
                                                                                                                                                                                                                                      aaTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtg                                                       >  2:59618/1‑182 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
CCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGA  >  NC_000913/3460554‑3461014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: