Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,499,607	A→C	17.1%	A54A (GCA→GCC)	yhfL →	small lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,607	0	A	C	17.1%	86.5 / 2.3	58	A54A (GCA→GCC)	yhfL	small lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (14/33);  new base C (8/3);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTG  >  NC_000913/3499375‑3499840                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      ttAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTaa                                                                                                                                                                                                                                                 <  2:387812/239‑1 (MQ=255)                                                         aCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTg                                                                                                                                                                                         <  2:506941/233‑1 (MQ=255)                                                             aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTAATGCACCAGAAATCTCTGAACG‑CACACGGCCAAC‑C‑CCCGTTTAT‑TTT‑CCC‑gcacccccccggtgttttccc                                                                                                                                                                               >  2:313193/1‑219 (MQ=255)                                                               ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGTACAGTAAAACTCTTACAG‑AAAAAGGGTAAA‑A‑CCGGGTTAA‑TTT‑TCC‑GCGGCCCTCCGGATTTTT‑CGCgg                                                                                                                                                                            >  2:125505/1‑239 (MQ=255)                                                               ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCCACTGCCCCGTAAATCTTCGACAG‑CAATAGGCTAAC‑ACCCGGTTTAT‑TTT‑CCC‑‑ACGCCCTCCGGGtttttcccgg                                                                                                                                                                             >  2:997310/1‑233 (MQ=255)                                                                   tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGACTCTCACAG‑AAATAGGCTAAC‑A‑CCGGGTTTA‑TTT‑TCC‑GACGCCCTCTGGGTTTTT‑CCAGGGCgg                                                                                                                                                                        >  2:1250713/1‑238 (MQ=255)                                                                   tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAAGCTCCGACAG‑AAAATGGCTAAC‑A‑CCGGGTTAA‑TTGTACC‑GA‑GCCCCCTGGAGTTTT‑CGCgggggg                                                                                                                                                                        >  2:17297/1‑236 (MQ=255)                                                                                                gtgtgggttttgtaaagagacaagTTAAATGTATTTACGGGTCATATTGAAAACCGTAATAAGAACTGCTCTTACGACTACCGGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCcccgc                                                                                                                                       <  1:847671/216‑1 (MQ=255)                                                                                                   gTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGGCCTACTGCACACAAAATCTCTTACCG‑AAAACGGCTAAC‑A‑CCCGTTTAT‑ATT‑TCC‑GCCACCCTCTGGGTTTTT‑CCCGTGCGCG‑TTTTTTTCTCTGTGCTTC‑TTT‑‑Ccccccccc                                                                                                                                     >  2:1269312/1‑238 (MQ=255)                                                                                                        tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACTGCCCAAAAAATCTCTTACAGAAAATCGG‑TCAC‑A‑CCCGGTTTA‑TTG‑CCC‑CCCGCCCCCCTGAATTTC‑CGAGGGCGGC‑TTTTTTTCTTTGTCCCTC‑TTT‑‑Ccccccccccccaaa                                                                                                                               >  2:498428/1‑233 (MQ=255)                                                                                                        tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTCCTGCCCAGTAAGTCTCTGACAG‑AAAACGGCTAAC‑A‑CCCGTTTTA‑TTT‑CCC‑CGCGCCCCCCGGAGTTTT‑CCAGGGCGGC‑TTTGTTTCTTTTTCATTC‑TTT‑‑CCcccgcccctccaa                                                                                                                               >  2:849878/1‑233 (MQ=255)                                                                                                             tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACCGTAAGTCTCTGAAAG‑AAAAACGCCAAC‑A‑CCCGATTTA‑TTT‑CCC‑CACGCCCTCTGGGTTTTT‑CTCGGGCGGA‑TTTGTTTTTTTTTTCTTC‑TTA‑‑CCCCCCCCCGTCAAAAAAtt                                                                                                                          >  2:928065/1‑239 (MQ=255)                                                                                                             tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGTGCCGCTGCACAATAAATATCTGACAT‑AAACTGGCTAAC‑C‑CCGTTGTTA‑TTT‑ACC‑GACGCCCCCTGGATGTTT‑CGTGGGGGGA‑TTTGTTTTTTTGTCCTTC‑TTG‑‑Ccccccccctccaaaaaatt                                                                                                                          >  2:249099/1‑228 (MQ=255)                                                                                                             tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCATAAAACTCTGACAG‑AAATAGGCTAAC‑A‑CCCGGTTAA‑TTT‑CCC‑GCCGCCCCCTGGGTTTTT‑CTAGGGCGGA‑TTTGTTTTTTTGTCCTTCTTTG‑‑CCCCCCCCCCCaaaaaatt                                                                                                                           >  2:998356/1‑236 (MQ=255)                                                                                                             tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACAGTCAGTCTCTGTCAG‑AAATAGGCTAAC‑A‑CCCGGTTTA‑TTT‑CCC‑GACGCCCCCTGTGGTTTT‑CTACGGCGGA‑TTTTTTTTTATGTCCTTT‑TTT‑‑Ccccccccccccaaaaaatt                                                                                                                          >  2:1250570/1‑228 (MQ=255)                                                                                                             tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGGCCCACAGCCCCATAAATTTCTGACAG‑AAATACGCTAAA‑A‑CCCGGTTTA‑TTT‑CCC‑GGCTCCCCCTGTGCTTTC‑CCCGAGTGCA‑TGTGTTTTTATGTCCCTC‑CTGTCCCCCCCCCCGCAAAAatt                                                                                                                            >  2:1184080/1‑238 (MQ=255)                                                                                                                  gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCCCACTCAGTCTTTTGCAG‑AAAATCGCTTAC‑A‑CCCGTTTTA‑TTT‑CCCGGCCCCCCCCTGGTTTTTC‑TGAGGGGGAT‑TTTTTTTTTTTTCCCTTT‑TTC‑‑CCcccccccgaccacatttttt                                                                                                                        >  2:669330/1‑225 (MQ=255)                                                                                                                  gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCCTCACAATAAATCTCTCACCG‑AAAACGGGTCAC‑A‑CCCGGTTAC‑TTT‑GCC‑CCCGCCCTCTCGAGTTTC‑CGAGGGCGTA‑TTTGTTTTTATTTCCTTCTTTG‑‑CCCCCCCCCTCAAAAAAAtttc                                                                                                                        >  2:52323/1‑236 (MQ=255)                                                                                                                  gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCGTAAGTCTCTGACAG‑AAATCGGCTAAA‑C‑ACGGTTTAA‑TTT‑TCC‑GCCGCCCCCTCGGTTTTC‑CGAAGGCGGA‑TTTTTTTCTTTGTCCTTC‑TTG‑‑TCCCCGCCCCCTAAAAAAttttt                                                                                                                       >  2:211629/1‑236 (MQ=255)                                                                                                                       gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTAATGCCCCATAAATTTCTGACCG‑AAAACGGCTAAC‑A‑CCGGCTTAA‑TTT‑CCC‑GCAGCCCCCTGGGTTTTCTCAAGGGGGAT‑TTTTTTTTTTTTTCTTTT‑TTT‑‑CCCCCCCCCCCACAAAAATTTTCA‑TTTaa                                                                                                                >  2:630820/1‑239 (MQ=255)                                                                                                                                  gTACTGGTCATATTGAAAACCGTTATAAGAACTGCTCTTACGACTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                                     <  2:1132223/239‑1 (MQ=255)                                                                                                                                  gTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCCCACAAAATCTCTGACAG‑AAATACGCTAAC‑A‑CCGGGTTTA‑TTT‑TCC‑CCCGCCCCCTTGGGTTTT‑CTCGGGGGGG‑TTTGTTTTTTTTTTCTTC‑TTGTCCCCCCCCCCAAAAAAACATTTTCTTTTTaaactatcct                                                                                                        >  2:847671/1‑231 (MQ=255)                                                                                                                                   ttcTGGTCATTTTGAAAACCTTGATAAGAACTGCTTCTACGACTACCGGCTGCACCCGGCAATGTCTATTTCTAAAATCATTGGCTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                    <  1:997310/237‑1 (MQ=255)                                                                                                                                     cTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaa                                                                                                  <  2:981383/239‑1 (MQ=255)                                                                                                                                       ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTCCTGCACAGGAAATCTCTTACAG‑AAAACGGGTAAC‑A‑CCCGTTTTA‑TTG‑CCC‑CGCCCCCTCCGGGTTTTT‑CCAGGGCGGG‑TTTGTTTCTTTGTCCTTT‑TTT‑‑TCCCCCCCCCTCAAAAAAATTTCACTTTTaatttaatttttttaat                                                                                                >  2:1127628/1‑232 (MQ=255)                                                                                                                                         gtattttGAAAACCGTGATAAGAACTGCCCTTACGGCTTCCTTCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  1:52323/234‑1 (MQ=255)                                                                                                                                          cATTTTGAAAACCGTGATAAGAACTTGTTTTACGACTTCCTGCTCCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  1:998356/238‑1 (MQ=255)                                                                                                                                          cATATTGAAACCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  2:963601/238‑1 (MQ=255)                                                                                                                                          cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCTGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  2:843280/238‑1 (MQ=255)                                                                                                                                           atatTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACAt                                                                                            <  2:576871/239‑1 (MQ=255)                                                                                                                                                     cctttaaaaGAACTGCTTTTACGACTACCGGCTGCCCCCGGCAATTTCTATTTTCAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                  <  1:669330/232‑1 (MQ=255)                                                                                                                                                     cctgtaTAAAAATTGTTCTTCCGCTTTCCTGCTTCCCCCGGCAATTTATATTTCAAAATACATTGGGTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATACAATTAGATAACTACAGCCATTAGCca                                                                                  <  1:17297/234‑1 (MQ=255)                                                                                                                                                     ccGTGATAAGAACTTCTTTTTCGACTACCTGCCGCACCCGGCAATTTTTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                  <  1:1250713/239‑1 (MQ=255)                                                                                                                                                     ccGTGATAAAAACTGCCTTTACGGCTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                  <  1:1127628/239‑1 (MQ=255)                                                                                                                                                       gTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCCACTCCACAGTAAAGCTCTTACAG‑AAAACGGCTAAC‑A‑CCCGTTTAATTTC‑CCC‑CACCCCCCCTGGTTTTCC‑CGAGGG‑GTATTTTGTTTTTTTTCCTTTT‑TTC‑‑CCCCCCCCCGCAAAAAAATTTTACTTTTAATTTCAAttttttaaatcctccctttgcccca                                                                                 >  2:593223/1‑217 (MQ=255)                                                                                                                                                               accTGCTCTTACGTCTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                                                                        <  1:249099/237‑1 (MQ=255)                                                                                                                                                                aCTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGGCCTCCCGCCCAGAAAAGATCTGACAA‑AAAACAGCTAAC‑A‑CCGGGTTTA‑TTT‑CCC‑CACGCCCCCTGGGTTTTT‑CTACGGGGGC‑TTTGTTTTTATTTTCTTT‑TTT‑‑GCCCCCCCCCCGAAAACATTTTTACCTTAAATTCAATTTATTTAAAACAACCATTAGAcaaaaaccgat                                                                         >  1:1110921/1‑228 (MQ=255)                                                                                                                                                                              tACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAGTCTCTTACAG‑AAAAAGGCTAACAA‑CCGGTTTAT‑TTC‑CCG‑GCCCCCCCCGGGTTTTTC‑CGGGGGGGAT‑TTTGTTTTTTTTTCTTTT‑TTC‑‑CCCCCCCCCGACAAAAATTTTTCATTTTAATTTCATTTTG‑TAACTCACTCCCTTTACCCCACCGCCGTCCTTGTGCGAATtttt                                                         >  2:814400/1‑239 (MQ=255)                                                                                                                                                                                    ctgccaccGGCAATTTCTTTTTCTAAAATCCTTGGCGGTGGCGGTCCTTCTGCACAGTAAGTCGCTGGCAG‑AAATCGGCTAAC‑G‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                                                                    <  1:193729/200‑1 (MQ=255)                                                                                                                                                                                      tccccccGCATTTTTTATTTCTAAAATCATTGGGTGTTTCGGTCCTTCTGCACAGTAAGTTCTTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCGGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTTACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtc                                                <  1:498428/236‑1 (MQ=255)                                                                                                                                                                                      gcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtc                                                <  2:655245/239‑1 (MQ=255)                                                                                                                                                                                        cccccgaattttttgtttttaAAAACATTGGCGGTTGCGGTCCTTCTGCCCAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑C‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTTGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtcct                                              <  1:814400/224‑1 (MQ=255)                                                                                                                                                                                        ccccGTCATTTTTTTTTTTTAAAATTATTGGCGGTTGCGGTTCTTCTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtcct                                              <  1:630820/238‑1 (MQ=255)                                                                                                                                                                                           cGGCAATTTTTTTTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAAGCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TGG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCca                                           <  1:849878/239‑1 (MQ=255)                                                                                                                                                                                             gctatttttatttctAAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGGTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCcaca                                         <  1:308220/236‑1 (MQ=255)                                                                                                                                                                                             gCAATTTCTATTTCTAAAATCATTGGCGTTTGCGGTCTTACTGCACAGTAAGTCTCGGAACG‑AAATCGGCTAAC‑C‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCcaca                                         <  1:1269312/239‑1 (MQ=255)                                                                                                                                                                                              cAATTTTTTTTTTTAAAACTAGTGGCGGTTGGGGTCCTTCTTCACACTAAGTCCGTTACAG‑AAATCGGCTAAC‑T‑ACGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCGCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCcaca                                         <  1:928065/238‑1 (MQ=255)                                                                                                                                                                                                   ttttttttttaaaTCATTGGCGGTTGGGGTCCTACTGCCCAGGAAGGCTCGTACAG‑AAATCGGCTAAC‑C‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                   <  1:1250570/229‑1 (MQ=255)                                                                                                                                                                                                   tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTA‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                   <  2:629925/239‑1 (MQ=255)                                                                                                                                                                                                    ctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                  <  2:1110921/239‑1 (MQ=255)                                                                                                                                                                                                           taAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcat                           <  2:281442/239‑1 (MQ=255)                                                                                                                                                                                                            aaatataatggggtgtGCGGGCCTTCTGCACTGTAAGTCTCTGAAAG‑AAAACGGCTAAC‑T‑ACGGTTTAA‑TTG‑CCC‑GACGCCCCCTGGATTTTC‑CGAGGGCTTA‑TTTTTTTCTAGGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTGCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                          <  1:1184080/224‑1 (MQ=255)                                                                                                                                                                                                            aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                          <  2:192210/239‑1 (MQ=255)                                                                                                                                                                                                              aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                        <  2:316955/239‑1 (MQ=255)                                                                                                                                                                                                              aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                        <  2:912682/239‑1 (MQ=255)                                                                                                                                                                                                                              ggTCCTACTGCACAGTAAGTCCCTGACAG‑AAATCGGCTAAC‑T‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtcct                                              <  2:885265/201‑1 (MQ=255)                                                                                                                                                                                                                                    aCTGCCCAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg  <  2:774534/239‑1 (MQ=255)                                                                                                                                                                                                                                    aCTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg  <  2:258600/239‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑A‑CCGGTTTAA‑TTG‑CCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTG  >  NC_000913/3499375‑3499840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑