Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,499,607 A→C 17.1% A54A (GCA→GCC yhfL → small lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,6070AC17.1% 86.5 / 2.3 58A54A (GCA→GCCyhfLsmall lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (14/33);  new base C (8/3);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTG  >  NC_000913/3499375‑3499840
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     
ttAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTaa                                                                                                                                                                                                                                                 <  2:387812/239‑1 (MQ=255)
                                                        aCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTg                                                                                                                                                                                         <  2:506941/233‑1 (MQ=255)
                                                            aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTAATGCACCAGAAATCTCTGAACGCACACGGCCAACCCCCGTTTATTTTCCCgcacccccccggtgttttccc                                                                                                                                                                               >  2:313193/1‑219 (MQ=255)
                                                              ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGTACAGTAAAACTCTTACAGAAAAAGGGTAAAACCGGGTTAATTTTCCGCGGCCCTCCGGATTTTTCGCgg                                                                                                                                                                            >  2:125505/1‑239 (MQ=255)
                                                              ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCCACTGCCCCGTAAATCTTCGACAGCAATAGGCTAACACCCGGTTTATTTTCCCACGCCCTCCGGGtttttcccgg                                                                                                                                                                             >  2:997310/1‑233 (MQ=255)
                                                                  tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGACTCTCACAGAAATAGGCTAACACCGGGTTTATTTTCCGACGCCCTCTGGGTTTTTCCAGGGCgg                                                                                                                                                                        >  2:1250713/1‑238 (MQ=255)
                                                                  tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAAGCTCCGACAGAAAATGGCTAACACCGGGTTAATTGTACCGAGCCCCCTGGAGTTTTCGCgggggg                                                                                                                                                                        >  2:17297/1‑236 (MQ=255)
                                                                                               gtgtgggttttgtaaagagacaagTTAAATGTATTTACGGGTCATATTGAAAACCGTAATAAGAACTGCTCTTACGACTACCGGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCcccgc                                                                                                                                       <  1:847671/216‑1 (MQ=255)
                                                                                                  gTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGGCCTACTGCACACAAAATCTCTTACCGAAAACGGCTAACACCCGTTTATATTTCCGCCACCCTCTGGGTTTTTCCCGTGCGCGTTTTTTTCTCTGTGCTTCTTT‑‑Ccccccccc                                                                                                                                     >  2:1269312/1‑238 (MQ=255)
                                                                                                       tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACTGCCCAAAAAATCTCTTACAGAAAATCGGTCACACCCGGTTTATTGCCCCCCGCCCCCCTGAATTTCCGAGGGCGGCTTTTTTTCTTTGTCCCTCTTT‑‑Ccccccccccccaaa                                                                                                                               >  2:498428/1‑233 (MQ=255)
                                                                                                       tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTCCTGCCCAGTAAGTCTCTGACAGAAAACGGCTAACACCCGTTTTATTTCCCCGCGCCCCCCGGAGTTTTCCAGGGCGGCTTTGTTTCTTTTTCATTCTTT‑‑CCcccgcccctccaa                                                                                                                               >  2:849878/1‑233 (MQ=255)
                                                                                                            tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACCGTAAGTCTCTGAAAGAAAAACGCCAACACCCGATTTATTTCCCCACGCCCTCTGGGTTTTTCTCGGGCGGATTTGTTTTTTTTTTCTTCTTA‑‑CCCCCCCCCGTCAAAAAAtt                                                                                                                          >  2:928065/1‑239 (MQ=255)
                                                                                                            tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGTGCCGCTGCACAATAAATATCTGACATAAACTGGCTAACCCCGTTGTTATTTACCGACGCCCCCTGGATGTTTCGTGGGGGGATTTGTTTTTTTGTCCTTCTTG‑‑Ccccccccctccaaaaaatt                                                                                                                          >  2:249099/1‑228 (MQ=255)
                                                                                                            tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCATAAAACTCTGACAGAAATAGGCTAACACCCGGTTAATTTCCCGCCGCCCCCTGGGTTTTTCTAGGGCGGATTTGTTTTTTTGTCCTTCTTTG‑‑CCCCCCCCCCCaaaaaatt                                                                                                                           >  2:998356/1‑236 (MQ=255)
                                                                                                            tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACAGTCAGTCTCTGTCAGAAATAGGCTAACACCCGGTTTATTTCCCGACGCCCCCTGTGGTTTTCTACGGCGGATTTTTTTTTATGTCCTTTTTT‑‑Ccccccccccccaaaaaatt                                                                                                                          >  2:1250570/1‑228 (MQ=255)
                                                                                                            tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGGCCCACAGCCCCATAAATTTCTGACAGAAATACGCTAAAACCCGGTTTATTTCCCGGCTCCCCCTGTGCTTTCCCCGAGTGCATGTGTTTTTATGTCCCTCCTGTCCCCCCCCCCGCAAAAatt                                                                                                                            >  2:1184080/1‑238 (MQ=255)
                                                                                                                 gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCCCACTCAGTCTTTTGCAGAAAATCGCTTACACCCGTTTTATTTCCCGGCCCCCCCCTGGTTTTTCTGAGGGGGATTTTTTTTTTTTTCCCTTTTTC‑‑CCcccccccgaccacatttttt                                                                                                                        >  2:669330/1‑225 (MQ=255)
                                                                                                                 gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCCTCACAATAAATCTCTCACCGAAAACGGGTCACACCCGGTTACTTTGCCCCCGCCCTCTCGAGTTTCCGAGGGCGTATTTGTTTTTATTTCCTTCTTTG‑‑CCCCCCCCCTCAAAAAAAtttc                                                                                                                        >  2:52323/1‑236 (MQ=255)
                                                                                                                 gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCGTAAGTCTCTGACAGAAATCGGCTAAACACGGTTTAATTTTCCGCCGCCCCCTCGGTTTTCCGAAGGCGGATTTTTTTCTTTGTCCTTCTTG‑‑TCCCCGCCCCCTAAAAAAttttt                                                                                                                       >  2:211629/1‑236 (MQ=255)
                                                                                                                      gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTAATGCCCCATAAATTTCTGACCGAAAACGGCTAACACCGGCTTAATTTCCCGCAGCCCCCTGGGTTTTCTCAAGGGGGATTTTTTTTTTTTTTCTTTTTTT‑‑CCCCCCCCCCCACAAAAATTTTCATTTaa                                                                                                                >  2:630820/1‑239 (MQ=255)
                                                                                                                                 gTACTGGTCATATTGAAAACCGTTATAAGAACTGCTCTTACGACTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                                     <  2:1132223/239‑1 (MQ=255)
                                                                                                                                 gTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCCCACAAAATCTCTGACAGAAATACGCTAACACCGGGTTTATTTTCCCCCGCCCCCTTGGGTTTTCTCGGGGGGGTTTGTTTTTTTTTTCTTCTTGTCCCCCCCCCCAAAAAAACATTTTCTTTTTaaactatcct                                                                                                        >  2:847671/1‑231 (MQ=255)
                                                                                                                                  ttcTGGTCATTTTGAAAACCTTGATAAGAACTGCTTCTACGACTACCGGCTGCACCCGGCAATGTCTATTTCTAAAATCATTGGCTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                    <  1:997310/237‑1 (MQ=255)
                                                                                                                                    cTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaa                                                                                                  <  2:981383/239‑1 (MQ=255)
                                                                                                                                      ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTCCTGCACAGGAAATCTCTTACAGAAAACGGGTAACACCCGTTTTATTGCCCCGCCCCCTCCGGGTTTTTCCAGGGCGGGTTTGTTTCTTTGTCCTTTTTT‑‑TCCCCCCCCCTCAAAAAAATTTCACTTTTaatttaatttttttaat                                                                                                >  2:1127628/1‑232 (MQ=255)
                                                                                                                                        gtattttGAAAACCGTGATAAGAACTGCCCTTACGGCTTCCTTCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  1:52323/234‑1 (MQ=255)
                                                                                                                                         cATTTTGAAAACCGTGATAAGAACTTGTTTTACGACTTCCTGCTCCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  1:998356/238‑1 (MQ=255)
                                                                                                                                         cATATTGAAACCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  2:963601/238‑1 (MQ=255)
                                                                                                                                         cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCTGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                              <  2:843280/238‑1 (MQ=255)
                                                                                                                                          atatTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACAt                                                                                            <  2:576871/239‑1 (MQ=255)
                                                                                                                                                    cctttaaaaGAACTGCTTTTACGACTACCGGCTGCCCCCGGCAATTTCTATTTTCAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                  <  1:669330/232‑1 (MQ=255)
                                                                                                                                                    cctgtaTAAAAATTGTTCTTCCGCTTTCCTGCTTCCCCCGGCAATTTATATTTCAAAATACATTGGGTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATACAATTAGATAACTACAGCCATTAGCca                                                                                  <  1:17297/234‑1 (MQ=255)
                                                                                                                                                    ccGTGATAAGAACTTCTTTTTCGACTACCTGCCGCACCCGGCAATTTTTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                  <  1:1250713/239‑1 (MQ=255)
                                                                                                                                                    ccGTGATAAAAACTGCCTTTACGGCTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                  <  1:1127628/239‑1 (MQ=255)
                                                                                                                                                      gTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCCACTCCACAGTAAAGCTCTTACAGAAAACGGCTAACACCCGTTTAATTTCCCCCACCCCCCCTGGTTTTCCCGAGGGGTATTTTGTTTTTTTTCCTTTTTTC‑‑CCCCCCCCCGCAAAAAAATTTTACTTTTAATTTCAAttttttaaatcctccctttgcccca                                                                                 >  2:593223/1‑217 (MQ=255)
                                                                                                                                                              accTGCTCTTACGTCTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                                                                        <  1:249099/237‑1 (MQ=255)
                                                                                                                                                               aCTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGGCCTCCCGCCCAGAAAAGATCTGACAAAAAACAGCTAACACCGGGTTTATTTCCCCACGCCCCCTGGGTTTTTCTACGGGGGCTTTGTTTTTATTTTCTTTTTT‑‑GCCCCCCCCCCGAAAACATTTTTACCTTAAATTCAATTTATTTAAAACAACCATTAGAcaaaaaccgat                                                                         >  1:1110921/1‑228 (MQ=255)
                                                                                                                                                                             tACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAGTCTCTTACAGAAAAAGGCTAACAACCGGTTTATTTCCCGGCCCCCCCCGGGTTTTTCCGGGGGGGATTTTGTTTTTTTTTCTTTTTTC‑‑CCCCCCCCCGACAAAAATTTTTCATTTTAATTTCATTTTGTAACTCACTCCCTTTACCCCACCGCCGTCCTTGTGCGAATtttt                                                         >  2:814400/1‑239 (MQ=255)
                                                                                                                                                                                   ctgccaccGGCAATTTCTTTTTCTAAAATCCTTGGCGGTGGCGGTCCTTCTGCACAGTAAGTCGCTGGCAGAAATCGGCTAACGCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                                                                    <  1:193729/200‑1 (MQ=255)
                                                                                                                                                                                     tccccccGCATTTTTTATTTCTAAAATCATTGGGTGTTTCGGTCCTTCTGCACAGTAAGTTCTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCGGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTTACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                <  1:498428/236‑1 (MQ=255)
                                                                                                                                                                                     gcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                <  2:655245/239‑1 (MQ=255)
                                                                                                                                                                                       cccccgaattttttgtttttaAAAACATTGGCGGTTGCGGTCCTTCTGCCCAGTAAGTCTCTGACAGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTTGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                              <  1:814400/224‑1 (MQ=255)
                                                                                                                                                                                       ccccGTCATTTTTTTTTTTTAAAATTATTGGCGGTTGCGGTTCTTCTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                              <  1:630820/238‑1 (MQ=255)
                                                                                                                                                                                          cGGCAATTTTTTTTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAAGCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTGG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                           <  1:849878/239‑1 (MQ=255)
                                                                                                                                                                                            gctatttttatttctAAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGGTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                         <  1:308220/236‑1 (MQ=255)
                                                                                                                                                                                            gCAATTTCTATTTCTAAAATCATTGGCGTTTGCGGTCTTACTGCACAGTAAGTCTCGGAACGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                         <  1:1269312/239‑1 (MQ=255)
                                                                                                                                                                                             cAATTTTTTTTTTTAAAACTAGTGGCGGTTGGGGTCCTTCTTCACACTAAGTCCGTTACAGAAATCGGCTAACTACGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCGCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                         <  1:928065/238‑1 (MQ=255)
                                                                                                                                                                                                  ttttttttttaaaTCATTGGCGGTTGGGGTCCTACTGCCCAGGAAGGCTCGTACAGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                   <  1:1250570/229‑1 (MQ=255)
                                                                                                                                                                                                  tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTA‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                   <  2:629925/239‑1 (MQ=255)
                                                                                                                                                                                                   ctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                  <  2:1110921/239‑1 (MQ=255)
                                                                                                                                                                                                          taAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcat                           <  2:281442/239‑1 (MQ=255)
                                                                                                                                                                                                           aaatataatggggtgtGCGGGCCTTCTGCACTGTAAGTCTCTGAAAGAAAACGGCTAACTACGGTTTAATTGCCCGACGCCCCCTGGATTTTCCGAGGGCTTATTTTTTTCTAGGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTGCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                          <  1:1184080/224‑1 (MQ=255)
                                                                                                                                                                                                           aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                          <  2:192210/239‑1 (MQ=255)
                                                                                                                                                                                                             aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                        <  2:316955/239‑1 (MQ=255)
                                                                                                                                                                                                             aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                        <  2:912682/239‑1 (MQ=255)
                                                                                                                                                                                                                             ggTCCTACTGCACAGTAAGTCCCTGACAGAAATCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                              <  2:885265/201‑1 (MQ=255)
                                                                                                                                                                                                                                   aCTGCCCAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg  <  2:774534/239‑1 (MQ=255)
                                                                                                                                                                                                                                   aCTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg  <  2:258600/239‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     
TTAACGAGCAGTCTGCTTATATTCATATAATCAATGAATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTG  >  NC_000913/3499375‑3499840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: