Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,499,649 G→T 24.0% intergenic (+36/‑259) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,6490GT24.0% 138.1 / 7.1 76intergenic (+36/‑259)yhfL/frlAsmall lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (12/43);  new base T (17/2);  total (31/45)
Fisher's exact test for biased strand distribution p-value = 2.47e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

ACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTA  >  NC_000913/3499431‑3499884
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
aCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTg                                                                                                                                                                                                                                     <  2:506941/233‑1 (MQ=255)
    aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTAATGCACCAGAAATCTCTGAACGCACACGGCCAACCCCCGTTTATTTTCCCgcacccccccggtgttttccc                                                                                                                                                                                                                           >  2:313193/1‑219 (MQ=255)
      ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGTACAGTAAAACTCTTACAGAAAAAGGGTAAAACCGGGTTAATTTTCCGCGGCCCTCCGGATTTTTCGCgg                                                                                                                                                                                                                        >  2:125505/1‑239 (MQ=255)
      ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCCACTGCCCCGTAAATCTTCGACAGCAATAGGCTAACACCCGGTTTATTTTCCCACGCCCTCCGGGtttttcccgg                                                                                                                                                                                                                         >  2:997310/1‑233 (MQ=255)
          tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGACTCTCACAGAAATAGGCTAACACCGGGTTTATTTTCCGACGCCCTCTGGGTTTTTCCAGGGCgg                                                                                                                                                                                                                    >  2:1250713/1‑238 (MQ=255)
          tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAAGCTCCGACAGAAAATGGCTAACACCGGGTTAATTGTACCGAGCCCCCTGGAGTTTTCGCgggggg                                                                                                                                                                                                                    >  2:17297/1‑236 (MQ=255)
                                       gtgtgggttttgtaaagagacaagTTAAATGTATTTACGGGTCATATTGAAAACCGTAATAAGAACTGCTCTTACGACTACCGGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCcccgc                                                                                                                                                                                   <  1:847671/216‑1 (MQ=255)
                                          gTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGGCCTACTGCACACAAAATCTCTTACCGAAAACGGCTAACACCCGTTTATATTTCCGCCACCCTCTGGGTTTTTCCCGTGCGCGTTTTTTTCTCTGTGCTTCTTT‑‑Ccccccccc                                                                                                                                                                                 >  2:1269312/1‑238 (MQ=255)
                                               tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACTGCCCAAAAAATCTCTTACAGAAAATCGGTCACACCCGGTTTATTGCCCCCCGCCCCCCTGAATTTCCGAGGGCGGCTTTTTTTCTTTGTCCCTCTTT‑‑Ccccccccccccaaa                                                                                                                                                                           >  2:498428/1‑233 (MQ=255)
                                               tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTCCTGCCCAGTAAGTCTCTGACAGAAAACGGCTAACACCCGTTTTATTTCCCCGCGCCCCCCGGAGTTTTCCAGGGCGGCTTTGTTTCTTTTTCATTCTTT‑‑CCcccgcccctccaa                                                                                                                                                                           >  2:849878/1‑233 (MQ=255)
                                                    tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACCGTAAGTCTCTGAAAGAAAAACGCCAACACCCGATTTATTTCCCCACGCCCTCTGGGTTTTTCTCGGGCGGATTTGTTTTTTTTTTCTTCTTA‑‑CCCCCCCCCGTCAAAAAAtt                                                                                                                                                                      >  2:928065/1‑239 (MQ=255)
                                                    tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGTGCCGCTGCACAATAAATATCTGACATAAACTGGCTAACCCCGTTGTTATTTACCGACGCCCCCTGGATGTTTCGTGGGGGGATTTGTTTTTTTGTCCTTCTTG‑‑Ccccccccctccaaaaaatt                                                                                                                                                                      >  2:249099/1‑228 (MQ=255)
                                                    tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCATAAAACTCTGACAGAAATAGGCTAACACCCGGTTAATTTCCCGCCGCCCCCTGGGTTTTTCTAGGGCGGATTTGTTTTTTTGTCCTTCTTTG‑‑CCCCCCCCCCCaaaaaatt                                                                                                                                                                       >  2:998356/1‑236 (MQ=255)
                                                    tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACAGTCAGTCTCTGTCAGAAATAGGCTAACACCCGGTTTATTTCCCGACGCCCCCTGTGGTTTTCTACGGCGGATTTTTTTTTATGTCCTTTTTT‑‑Ccccccccccccaaaaaatt                                                                                                                                                                      >  2:1250570/1‑228 (MQ=255)
                                                    tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGGCCCACAGCCCCATAAATTTCTGACAGAAATACGCTAAAACCCGGTTTATTTCCCGGCTCCCCCTGTGCTTTCCCCGAGTGCATGTGTTTTTATGTCCCTCCTGTCCCCCCCCCCGCAAAAatt                                                                                                                                                                        >  2:1184080/1‑238 (MQ=255)
                                                         gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCCCACTCAGTCTTTTGCAGAAAATCGCTTACACCCGTTTTATTTCCCGGCCCCCCCCTGGTTTTTCTGAGGGGGATTTTTTTTTTTTTCCCTTTTTC‑‑CCcccccccgaccacatttttt                                                                                                                                                                    >  2:669330/1‑225 (MQ=255)
                                                         gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCCTCACAATAAATCTCTCACCGAAAACGGGTCACACCCGGTTACTTTGCCCCCGCCCTCTCGAGTTTCCGAGGGCGTATTTGTTTTTATTTCCTTCTTTG‑‑CCCCCCCCCTCAAAAAAAtttc                                                                                                                                                                    >  2:52323/1‑236 (MQ=255)
                                                         gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCGTAAGTCTCTGACAGAAATCGGCTAAACACGGTTTAATTTTCCGCCGCCCCCTCGGTTTTCCGAAGGCGGATTTTTTTCTTTGTCCTTCTTG‑‑TCCCCGCCCCCTAAAAAAttttt                                                                                                                                                                   >  2:211629/1‑236 (MQ=255)
                                                              gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTAATGCCCCATAAATTTCTGACCGAAAACGGCTAACACCGGCTTAATTTCCCGCAGCCCCCTGGGTTTTCTCAAGGGGGATTTTTTTTTTTTTTCTTTTTTT‑‑CCCCCCCCCCCACAAAAATTTTCATTTaa                                                                                                                                                            >  2:630820/1‑239 (MQ=255)
                                                                         gTACTGGTCATATTGAAAACCGTTATAAGAACTGCTCTTACGACTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                                                                                 <  2:1132223/239‑1 (MQ=255)
                                                                         gTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCCCACAAAATCTCTGACAGAAATACGCTAACACCGGGTTTATTTTCCCCCGCCCCCTTGGGTTTTCTCGGGGGGGTTTGTTTTTTTTTTCTTCTTGTCCCCCCCCCCAAAAAAACATTTTCTTTTTaaactatcct                                                                                                                                                    >  2:847671/1‑231 (MQ=255)
                                                                          ttcTGGTCATTTTGAAAACCTTGATAAGAACTGCTTCTACGACTACCGGCTGCACCCGGCAATGTCTATTTCTAAAATCATTGGCTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                                                                <  1:997310/237‑1 (MQ=255)
                                                                            cTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaa                                                                                                                                              <  2:981383/239‑1 (MQ=255)
                                                                              ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTCCTGCACAGGAAATCTCTTACAGAAAACGGGTAACACCCGTTTTATTGCCCCGCCCCCTCCGGGTTTTTCCAGGGCGGGTTTGTTTCTTTGTCCTTTTTT‑‑TCCCCCCCCCTCAAAAAAATTTCACTTTTaatttaatttttttaat                                                                                                                                            >  2:1127628/1‑232 (MQ=255)
                                                                                gtattttGAAAACCGTGATAAGAACTGCCCTTACGGCTTCCTTCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                          <  1:52323/234‑1 (MQ=255)
                                                                                 cATTTTGAAAACCGTGATAAGAACTTGTTTTACGACTTCCTGCTCCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                          <  1:998356/238‑1 (MQ=255)
                                                                                 cATATTGAAACCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                          <  2:963601/238‑1 (MQ=255)
                                                                                 cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCTGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                          <  2:843280/238‑1 (MQ=255)
                                                                                  atatTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACAt                                                                                                                                        <  2:576871/239‑1 (MQ=255)
                                                                                            cctttaaaaGAACTGCTTTTACGACTACCGGCTGCCCCCGGCAATTTCTATTTTCAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  1:669330/232‑1 (MQ=255)
                                                                                            cctgtaTAAAAATTGTTCTTCCGCTTTCCTGCTTCCCCCGGCAATTTATATTTCAAAATACATTGGGTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATACAATTAGATAACTACAGCCATTAGCca                                                                                                                              <  1:17297/234‑1 (MQ=255)
                                                                                            ccGTGATAAGAACTTCTTTTTCGACTACCTGCCGCACCCGGCAATTTTTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  1:1250713/239‑1 (MQ=255)
                                                                                            ccGTGATAAAAACTGCCTTTACGGCTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                              <  1:1127628/239‑1 (MQ=255)
                                                                                              gTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCCACTCCACAGTAAAGCTCTTACAGAAAACGGCTAACACCCGTTTAATTTCCCCCACCCCCCCTGGTTTTCCCGAGGGGTATTTTGTTTTTTTTCCTTTTTTC‑‑CCCCCCCCCGCAAAAAAATTTTACTTTTAATTTCAAttttttaaatcctccctttgcccca                                                                                                                             >  2:593223/1‑217 (MQ=255)
                                                                                                      accTGCTCTTACGTCTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                                                                                                                    <  1:249099/237‑1 (MQ=255)
                                                                                                       aCTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGGCCTCCCGCCCAGAAAAGATCTGACAAAAAACAGCTAACACCGGGTTTATTTCCCCACGCCCCCTGGGTTTTTCTACGGGGGCTTTGTTTTTATTTTCTTTTTT‑‑GCCCCCCCCCCGAAAACATTTTTACCTTAAATTCAATTTATTTAAAACAACCATTAGAcaaaaaccgat                                                                                                                     >  1:1110921/1‑228 (MQ=255)
                                                                                                                     tACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAGTCTCTTACAGAAAAAGGCTAACAACCGGTTTATTTCCCGGCCCCCCCCGGGTTTTTCCGGGGGGGATTTTGTTTTTTTTTCTTTTTTC‑‑CCCCCCCCCGACAAAAATTTTTCATTTTAATTTCATTTTGTAACTCACTCCCTTTACCCCACCGCCGTCCTTGTGCGAATtttt                                                                                                     >  2:814400/1‑239 (MQ=255)
                                                                                                                           ctgccaccGGCAATTTCTTTTTCTAAAATCCTTGGCGGTGGCGGTCCTTCTGCACAGTAAGTCGCTGGCAGAAATCGGCTAACGCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                                                                                                                <  1:193729/200‑1 (MQ=255)
                                                                                                                             tccccccGCATTTTTTATTTCTAAAATCATTGGGTGTTTCGGTCCTTCTGCACAGTAAGTTCTTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCGGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTTACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                                                            <  1:498428/236‑1 (MQ=255)
                                                                                                                             gcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc                                                                                            <  2:655245/239‑1 (MQ=255)
                                                                                                                               cccccgaattttttgtttttaAAAACATTGGCGGTTGCGGTCCTTCTGCCCAGTAAGTCTCTGACAGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTTGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                          <  1:814400/224‑1 (MQ=255)
                                                                                                                               ccccGTCATTTTTTTTTTTTAAAATTATTGGCGGTTGCGGTTCTTCTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                          <  1:630820/238‑1 (MQ=255)
                                                                                                                                  cGGCAATTTTTTTTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAAGCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTGG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCca                                                                                       <  1:849878/239‑1 (MQ=255)
                                                                                                                                    gctatttttatttctAAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGGTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                     <  1:308220/236‑1 (MQ=255)
                                                                                                                                    gCAATTTCTATTTCTAAAATCATTGGCGTTTGCGGTCTTACTGCACAGTAAGTCTCGGAACGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                     <  1:1269312/239‑1 (MQ=255)
                                                                                                                                     cAATTTTTTTTTTTAAAACTAGTGGCGGTTGGGGTCCTTCTTCACACTAAGTCCGTTACAGAAATCGGCTAACTACGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCGCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                     <  1:928065/238‑1 (MQ=255)
                                                                                                                                          ttttttttttaaaTCATTGGCGGTTGGGGTCCTACTGCCCAGGAAGGCTCGTACAGAAATCGGCTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                               <  1:1250570/229‑1 (MQ=255)
                                                                                                                                          tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTA‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                               <  2:629925/239‑1 (MQ=255)
                                                                                                                                           ctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                              <  2:1110921/239‑1 (MQ=255)
                                                                                                                                                  taAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcat                                                                       <  2:281442/239‑1 (MQ=255)
                                                                                                                                                   aaatataatggggtgtGCGGGCCTTCTGCACTGTAAGTCTCTGAAAGAAAACGGCTAACTACGGTTTAATTGCCCGACGCCCCCTGGATTTTCCGAGGGCTTATTTTTTTCTAGGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTGCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                      <  1:1184080/224‑1 (MQ=255)
                                                                                                                                                   aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                      <  2:192210/239‑1 (MQ=255)
                                                                                                                                                     aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                    <  2:316955/239‑1 (MQ=255)
                                                                                                                                                     aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAtg                                                                    <  2:912682/239‑1 (MQ=255)
                                                                                                                                                                     ggTCCTACTGCACAGTAAGTCCCTGACAGAAATCGGCTAACTCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcct                                                                                          <  2:885265/201‑1 (MQ=255)
                                                                                                                                                                           cctctcacgTAAGTCTTTGACGAAATTGGGCTTACCTCGGTTTAATTTCCCCTCGTCTCCTGGTTTTGTCGAGGTCTTTTTTGTTTCTATTCCCTTCTTG‑‑CCCCCGTCCGTAAAAATATTTTCCCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGTGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg                                              <  1:313193/231‑1 (MQ=255)
                                                                                                                                                                           aCTGCCCAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg                                              <  2:774534/239‑1 (MQ=255)
                                                                                                                                                                           aCTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTg                                              <  2:258600/239‑1 (MQ=255)
                                                                                                                                                                                 caGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACCTTACATAACATCATATGTTGTTATAtt                                                          >  2:381701/1‑221 (MQ=255)
                                                                                                                                                                                  cggtattctcTGACAGAAATCTGCTAACATCTGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTTCTTCTTG‑‑CCTCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatc                                                                         <  1:449937/199‑1 (MQ=255)
                                                                                                                                                                                   gTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTCCATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcaaa                                                                      >  1:1326838/1‑205 (MQ=255)
                                                                                                                                                                                   gTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                      <  2:1326838/207‑1 (MQ=255)
                                                                                                                                                                                    tAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACCGCGCGATCCTGTGCGAAATTTTTTGATCTTCCTCCACATTACATAAAATCATATTTTGTTTTATTTATAATAcattttcatttt                                       >  1:1196185/1‑237 (MQ=255)
                                                                                                                                                                                    tAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTTTCATGTTa                                      >  2:202955/1‑238 (MQ=255)
                                                                                                                                                                                      aGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCt                                   >  2:600504/1‑239 (MQ=255)
                                                                                                                                                                                       gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                         <  2:389599/153‑1 (MQ=255)
                                                                                                                                                                                       gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                         >  1:389599/1‑153 (MQ=255)
                                                                                                                                                                                         ctctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtttt                                >  1:211946/1‑239 (MQ=255)
                                                                                                                                                                                                    aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTTTGCGAAATTTTGTGa                                                                                                 >  1:71489/1‑164 (MQ=255)
                                                                                                                                                                                                    aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa                                                                                                 <  2:71489/164‑1 (MQ=255)
                                                                                                                                                                                                      tCGGGTAACCCCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATTTCCTTCTTG‑‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCaaa                   <  2:890202/239‑1 (MQ=255)
                                                                                                                                                                                                           tAACCCTGGTTTAATTTCCCGTCCCCCTCTGGATTTTCCGAAGGTTTTTTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCCCTTTAAATTCAATTTTATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAActc                <  1:184481/238‑1 (MQ=255)
                                                                                                                                                                                                              cacCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTccc            <  2:1252955/239‑1 (MQ=255)
                                                                                                                                                                                                                     gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGTTAACTACATCCATTAGCCACAGCGCGATCCTTTTCGAAATTTTACGATCTTCTTCCACATTACATAACATCATATGTTGTTATATCCATCATGCATTGTCAGGTTACCTTTTAAATACTTGCAAACTccccccacca       >  1:425850/1‑235 (MQ=255)
                                                                                                                                                                                                                      tttAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACa       <  2:425850/238‑1 (MQ=255)
                                                                                                                                                                                                                          aTTGCCCGACCCCCTCGGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTC‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATGTGATAACTACATCCATTAGCCACAGCTCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCAGTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTa  <  2:842461/239‑1 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
ACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTA  >  NC_000913/3499431‑3499884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: