Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,499,692	G→T	13.4%	intergenic (+79/‑216)	yhfL → / → frlA	small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,692	0	G	T	13.4%	214.8 / 4.9	81	intergenic (+79/‑216)	yhfL/frlA	small lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (22/47);  new base T (9/3);  total (31/50)
Fisher's exact test for biased strand distribution p-value = 8.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGG  >  NC_000913/3499470‑3499912                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   gtgtgggttttgtaaagagacaagTTAAATGTATTTACGGGTCATATTGAAAACCGTAATAAGAACTGCTCTTACGACTACCGGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCcccgc                                                                                                                                                                                                                 <  1:847671/216‑1 (MQ=255)    gTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGGCCTACTGCACACAAAATCTCTTACCG‑AAAACGGCTAAC‑ACCCGTTTAT‑ATTTCC‑GCCACCCTCTGGGTTTTT‑CCCGTGCGCG‑TTTTTTTCTCTGTGCTTC‑TTT‑‑Ccccccccc                                                                                                                                                                                                               >  2:1269312/1‑238 (MQ=255)         tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCCACTGCCCAAAAAATCTCTTACAGAAAATCGG‑TCAC‑ACCCGGTTTA‑TTGCCC‑CCCGCCCCCCTGAATTTC‑CGAGGGCGGC‑TTTTTTTCTTTGTCCCTC‑TTT‑‑Ccccccccccccaaa                                                                                                                                                                                                         >  2:498428/1‑233 (MQ=255)         tGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTCCTGCCCAGTAAGTCTCTGACAG‑AAAACGGCTAAC‑ACCCGTTTTA‑TTTCCC‑CGCGCCCCCCGGAGTTTT‑CCAGGGCGGC‑TTTGTTTCTTTTTCATTC‑TTT‑‑CCcccgcccctccaa                                                                                                                                                                                                         >  2:849878/1‑233 (MQ=255)              tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGTCCTACTGCACCGTAAGTCTCTGAAAG‑AAAAACGCCAAC‑ACCCGATTTA‑TTTCCC‑CACGCCCTCTGGGTTTTT‑CTCGGGCGGA‑TTTGTTTTTTTTTTCTTC‑TTA‑‑CCCCCCCCCGTCAAAAAAtt                                                                                                                                                                                                    >  2:928065/1‑239 (MQ=255)              tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGTGCCGCTGCACAATAAATATCTGACAT‑AAACTGGCTAAC‑CCCGTTGTTA‑TTTACC‑GACGCCCCCTGGATGTTT‑CGTGGGGGGA‑TTTGTTTTTTTGTCCTTC‑TTG‑‑Ccccccccctccaaaaaatt                                                                                                                                                                                                    >  2:249099/1‑228 (MQ=255)              tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCATAAAACTCTGACAG‑AAATAGGCTAAC‑ACCCGGTTAA‑TTTCCC‑GCCGCCCCCTGGGTTTTT‑CTAGGGCGGA‑TTTGTTTTTTTGTCCTTCTTTG‑‑CCCCCCCCCCCaaaaaatt                                                                                                                                                                                                     >  2:998356/1‑236 (MQ=255)              tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACAGTCAGTCTCTGTCAG‑AAATAGGCTAAC‑ACCCGGTTTA‑TTTCCC‑GACGCCCCCTGTGGTTTT‑CTACGGCGGA‑TTTTTTTTTATGTCCTTT‑TTT‑‑Ccccccccccccaaaaaatt                                                                                                                                                                                                    >  2:1250570/1‑228 (MQ=255)              tACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCCGGCCCACAGCCCCATAAATTTCTGACAG‑AAATACGCTAAA‑ACCCGGTTTA‑TTTCCC‑GGCTCCCCCTGTGCTTTC‑CCCGAGTGCA‑TGTGTTTTTATGTCCCTC‑CTGTCCCCCCCCCCGCAAAAatt                                                                                                                                                                                                      >  2:1184080/1‑238 (MQ=255)                   gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTTCGGGCCTACTGCCCACTCAGTCTTTTGCAG‑AAAATCGCTTAC‑ACCCGTTTTA‑TTTCCCGGCCCCCCCCTGGTTTTTC‑TGAGGGGGAT‑TTTTTTTTTTTTCCCTTT‑TTC‑‑CCcccccccgaccacatttttt                                                                                                                                                                                                  >  2:669330/1‑225 (MQ=255)                   gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTCCCTCACAATAAATCTCTCACCG‑AAAACGGGTCAC‑ACCCGGTTAC‑TTTGCC‑CCCGCCCTCTCGAGTTTC‑CGAGGGCGTA‑TTTGTTTTTATTTCCTTCTTTG‑‑CCCCCCCCCTCAAAAAAAtttc                                                                                                                                                                                                  >  2:52323/1‑236 (MQ=255)                   gCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCACCGTAAGTCTCTGACAG‑AAATCGGCTAAA‑CACGGTTTAA‑TTTTCC‑GCCGCCCCCTCGGTTTTC‑CGAAGGCGGA‑TTTTTTTCTTTGTCCTTC‑TTG‑‑TCCCCGCCCCCTAAAAAAttttt                                                                                                                                                                                                 >  2:211629/1‑236 (MQ=255)                        gTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTAATGCCCCATAAATTTCTGACCG‑AAAACGGCTAAC‑ACCGGCTTAA‑TTTCCC‑GCAGCCCCCTGGGTTTTCTCAAGGGGGAT‑TTTTTTTTTTTTTCTTTT‑TTT‑‑CCCCCCCCCCCACAAAAATTTTCA‑TTTaa                                                                                                                                                                                          >  2:630820/1‑239 (MQ=255)                                   gTACTGGTCATATTGAAAACCGTTATAAGAACTGCTCTTACGACTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                                                                                                                                               <  2:1132223/239‑1 (MQ=255)                                   gTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTTCCCACAAAATCTCTGACAG‑AAATACGCTAAC‑ACCGGGTTTA‑TTTTCC‑CCCGCCCCCTTGGGTTTT‑CTCGGGGGGG‑TTTGTTTTTTTTTTCTTC‑TTGTCCCCCCCCCCAAAAAAACATTTTCTTTTTaaactatcct                                                                                                                                                                                  >  2:847671/1‑231 (MQ=255)                                    ttcTGGTCATTTTGAAAACCTTGATAAGAACTGCTTCTACGACTACCGGCTGCACCCGGCAATGTCTATTTCTAAAATCATTGGCTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                                                                                                                                              <  1:997310/237‑1 (MQ=255)                                      cTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaa                                                                                                                                                                            <  2:981383/239‑1 (MQ=255)                                        ggTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTCCTGCACAGGAAATCTCTTACAG‑AAAACGGGTAAC‑ACCCGTTTTA‑TTGCCC‑CGCCCCCTCCGGGTTTTT‑CCAGGGCGGG‑TTTGTTTCTTTGTCCTTT‑TTT‑‑TCCCCCCCCCTCAAAAAAATTTCACTTTTaatttaatttttttaat                                                                                                                                                                          >  2:1127628/1‑232 (MQ=255)                                          gtattttGAAAACCGTGATAAGAACTGCCCTTACGGCTTCCTTCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                        <  1:52323/234‑1 (MQ=255)                                           cATTTTGAAAACCGTGATAAGAACTTGTTTTACGACTTCCTGCTCCCCCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                        <  1:998356/238‑1 (MQ=255)                                           cATATTGAAACCCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                        <  2:963601/238‑1 (MQ=255)                                           cATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCTGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                                                                                                                                        <  2:843280/238‑1 (MQ=255)                                            atatTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACAt                                                                                                                                                                      <  2:576871/239‑1 (MQ=255)                                                      cctttaaaaGAACTGCTTTTACGACTACCGGCTGCCCCCGGCAATTTCTATTTTCAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                                                            <  1:669330/232‑1 (MQ=255)                                                      cctgtaTAAAAATTGTTCTTCCGCTTTCCTGCTTCCCCCGGCAATTTATATTTCAAAATACATTGGGTGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATACAATTAGATAACTACAGCCATTAGCca                                                                                                                                                            <  1:17297/234‑1 (MQ=255)                                                      ccGTGATAAGAACTTCTTTTTCGACTACCTGCCGCACCCGGCAATTTTTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                                                            <  1:1250713/239‑1 (MQ=255)                                                      ccGTGATAAAAACTGCCTTTACGGCTTCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCca                                                                                                                                                            <  1:1127628/239‑1 (MQ=255)                                                        gTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCCACTCCACAGTAAAGCTCTTACAG‑AAAACGGCTAAC‑ACCCGTTTAATTTCCCC‑CACCCCCCCTGGTTTTCC‑CGAGGG‑GTATTTTGTTTTTTTTCCTTTT‑TTC‑‑CCCCCCCCCGCAAAAAAATTTTACTTTTAATTTCAAttttttaaatcctccctttgcccca                                                                                                                                                           >  2:593223/1‑217 (MQ=255)                                                                accTGCTCTTACGTCTCCCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGGTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                                                                                                                                                  <  1:249099/237‑1 (MQ=255)                                                                 aCTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGTGGGCCTCCCGCCCAGAAAAGATCTGACAA‑AAAACAGCTAAC‑ACCGGGTTTA‑TTTCCC‑CACGCCCCCTGGGTTTTT‑CTACGGGGGC‑TTTGTTTTTATTTTCTTT‑TTT‑‑GCCCCCCCCCCGAAAACATTTTTACCTTAAATTCAATTTATTTAAAACAACCATTAGAcaaaaaccgat                                                                                                                                                   >  1:1110921/1‑228 (MQ=255)                                                                               tACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGGCCTACTGCACAGTAAGTCTCTTACAG‑AAAAAGGCTAACAACCGGTTTAT‑TTCCCG‑GCCCCCCCCGGGTTTTTC‑CGGGGGGGAT‑TTTGTTTTTTTTTCTTTT‑TTC‑‑CCCCCCCCCGACAAAAATTTTTCATTTTAATTTCATTTTG‑TAACTCACTCCCTTTACCCCACCGCCGTCCTTGTGCGAATtttt                                                                                                                                   >  2:814400/1‑239 (MQ=255)                                                                                     ctgccaccGGCAATTTCTTTTTCTAAAATCCTTGGCGGTGGCGGTCCTTCTGCACAGTAAGTCGCTGGCAG‑AAATCGGCTAAC‑GCCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                                                                                                                                              <  1:193729/200‑1 (MQ=255)                                                                                       tccccccGCATTTTTTATTTCTAAAATCATTGGGTGTTTCGGTCCTTCTGCACAGTAAGTTCTTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCGGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTTACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtc                                                                                                                          <  1:498428/236‑1 (MQ=255)                                                                                       gcACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtc                                                                                                                          <  2:655245/239‑1 (MQ=255)                                                                                         cccccgaattttttgtttttaAAAACATTGGCGGTTGCGGTCCTTCTGCCCAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑CCCGGTTTAA‑TTGCCC‑GACGCCCTCTTGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtcct                                                                                                                        <  1:814400/224‑1 (MQ=255)                                                                                         ccccGTCATTTTTTTTTTTTAAAATTATTGGCGGTTGCGGTTCTTCTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtcct                                                                                                                        <  1:630820/238‑1 (MQ=255)                                                                                            cGGCAATTTTTTTTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAAGCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TGG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCca                                                                                                                     <  1:849878/239‑1 (MQ=255)                                                                                              gctatttttatttctAAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGGTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                   <  1:308220/236‑1 (MQ=255)                                                                                              gCAATTTCTATTTCTAAAATCATTGGCGTTTGCGGTCTTACTGCACAGTAAGTCTCGGAACG‑AAATCGGCTAAC‑CCCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                   <  1:1269312/239‑1 (MQ=255)                                                                                               cAATTTTTTTTTTTAAAACTAGTGGCGGTTGGGGTCCTTCTTCACACTAAGTCCGTTACAG‑AAATCGGCTAAC‑TACGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCGCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCcaca                                                                                                                   <  1:928065/238‑1 (MQ=255)                                                                                                    ttttttttttaaaTCATTGGCGGTTGGGGTCCTACTGCCCAGGAAGGCTCGTACAG‑AAATCGGCTAAC‑CCCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                             <  1:1250570/229‑1 (MQ=255)                                                                                                    tctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTA‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACAt                                                                                                             <  2:629925/239‑1 (MQ=255)                                                                                                     ctatttctaAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATa                                                                                                            <  2:1110921/239‑1 (MQ=255)                                                                                                            taAAATCATTGGGGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcat                                                                                                     <  2:281442/239‑1 (MQ=255)                                                                                                             aaatataatggggtgtGCGGGCCTTCTGCACTGTAAGTCTCTGAAAG‑AAAACGGCTAAC‑TACGGTTTAA‑TTGCCC‑GACGCCCCCTGGATTTTC‑CGAGGGCTTA‑TTTTTTTCTAGGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTGCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                                                    <  1:1184080/224‑1 (MQ=255)                                                                                                             aaaaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                                                    <  2:192210/239‑1 (MQ=255)                                                                                                               aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAt‑g                                                                                                 <  2:316955/239‑1 (MQ=255)                                                                                                               aaTCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAt‑g                                                                                                 <  2:912682/239‑1 (MQ=255)                                                                                                                               ggTCCTACTGCACAGTAAGTCCCTGACAG‑AAATCGGCTAAC‑TCCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTtcct                                                                                                                        <  2:885265/201‑1 (MQ=255)                                                                                                                                     cctctcacgTAAGTCTTTGACGA‑AATTGGGCTTAC‑CTCGGTTTAA‑TTTCCC‑CTCGTCTCCTGGTTTTGT‑CGAGGTCTTT‑TTTGTTTCTATTCCCTTC‑TTG‑‑CCCCCGTCCGTAAAAATATTTTCCCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGTGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTg                                                                           <  1:313193/231‑1 (MQ=255)                                                                                                                                     aCTGCCCAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTg                                                                           <  2:774534/239‑1 (MQ=255)                                                                                                                                     aCTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTg                                                                           <  2:258600/239‑1 (MQ=255)                                                                                                                                           caGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACCTTACATAACATCATAT‑GTTGTTATAtt                                                                                       >  2:381701/1‑221 (MQ=255)                                                                                                                                            cggtattctcTGACAG‑AAATCTGCTAAC‑ATCTGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTTCTTC‑TTG‑‑CCTCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatc                                                                                                       <  1:449937/199‑1 (MQ=255)                                                                                                                                             gTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTCCATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAAcatcaaa                                                                                                    >  1:1326838/1‑205 (MQ=255)                                                                                                                                             gTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCata                                                                                                    <  2:1326838/207‑1 (MQ=255)                                                                                                                                              tAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACCGCGCGATCC‑TGTGCGAAATTTTTTGATCTTCCTCCACATTACATAAAATCATAT‑TTTGTTTTATTTATAATAcattttcatttt                                                                    >  1:1196185/1‑237 (MQ=255)                                                                                                                                              tAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTTTCATGTTa                                                                   >  2:202955/1‑238 (MQ=255)                                                                                                                                                aGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCt                                                                >  2:600504/1‑239 (MQ=255)                                                                                                                                                 gTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                                                       <  2:389599/153‑1 (MQ=255)                                                                                                                                                 gTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                                                                                                                                       >  1:389599/1‑153 (MQ=255)                                                                                                                                                   ctctGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCtttt                                                             >  1:211946/1‑239 (MQ=255)                                                                                                                                                              aaTCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TTTGCGAAATTTTGTGa                                                                                                                               >  1:71489/1‑164 (MQ=255)                                                                                                                                                              aaTCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGa                                                                                                                               <  2:71489/164‑1 (MQ=255)                                                                                                                                                                tCGGGTAAC‑CCCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATTTCCTTC‑TTG‑‑‑CCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCaaa                                                <  2:890202/239‑1 (MQ=255)                                                                                                                                                                     tAAC‑CCTGGTTTAA‑TTTCCC‑GTCCCCCTCTGGATTTTC‑CGAAGGTTTT‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCCCTTTAAATTCAATTTTATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAAct‑c                                            <  1:184481/238‑1 (MQ=255)                                                                                                                                                                        c‑acCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTccc                                        <  2:1252955/239‑1 (MQ=255)                                                                                                                                                                              ttaaagtccagccccatccccTTTGTATTTTC‑CAAGGGCTTT‑TTTGTTTCTATGTCCTTC‑TTT‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTa                                     <  1:108200/221‑1 (MQ=255)                                                                                                                                                                              gTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGTTAACTACATCCATTAGCCACAGCGCGATCC‑TTTTCGAAATTTTACGATCTTCTTCCACATTACATAACATCATAT‑GTTGTTATATCCATCATGCATTGTCAGGTTACCTTTTAAATACTTGCAAACT‑ccccccacca                                   >  1:425850/1‑235 (MQ=255)                                                                                                                                                                               tttAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACa                                   <  2:425850/238‑1 (MQ=255)                                                                                                                                                                                   a‑TTGCCC‑GACCCCCTCGGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTC‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATGTGATAACTACATCCATTAGCCACAGCTCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCAGTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTa                              <  2:842461/239‑1 (MQ=255)                                                                                                                                                                                         cc‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCAtt                                                                            <  2:748896/189‑1 (MQ=255)                                                                                                                                                                                         cc‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCAtt                                                                            >  1:748896/1‑189 (MQ=255)                                                                                                                                                                                           agacagccTTTGGATTTTC‑CGAGGGCTTT‑TTTTTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTATTGGAc                        <  1:270427/233‑1 (MQ=255)                                                                                                                                                                                                   tctGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGCCCTTC‑TTG‑‑CCCCCACCCGTAAAAGTATTTTCACTTTAAATTCAATTTGAAAACTACACCCATTAACCACACGGCGATCC‑CGGGCGAAATTTTGTGATCTACCACCCCATTAATTAACA‑CATATAGTTGTTATTTTCATAATGCCTCTCCATGTGACCTTTTAAATGCATGCAAACTACTccctaaaaatatttgaaaaagaaaaa                 >  1:620455/1‑216 (MQ=255)                                                                                                                                                                                                    ctGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTATTg                           >  2:108200/1‑227 (MQ=255)                                                                                                                                                                                                       gATTTTC‑CGAGGGCGTT‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAAATCAATTTGATAACTGCATCCATTCGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTATTGGACAAGAAAAAGGTa            <  2:620455/239‑1 (MQ=255)                                                                                                                                                                                                            tC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTATTGGACAAGAAAAAGGtctgtct       >  2:270427/1‑233 (MQ=255)                                                                                                                                                                                                                     gctgtttttttTCTTTTTCCTTT‑TTT‑‑CCCCCCCCCGTAAAAATATTTTCTCTTTTAATTCAATTTGATAACTACATCCATTAGCCACAACCCGATCC‑TGTGCTAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATggg  <  1:125505/232‑1 (MQ=255)                                                                                                                                                                                                                                    tGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCAt                                                                             <  2:1291660/148‑1 (MQ=255)                                                                                                                                                                                                                                    tGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCAt                                                                             >  1:1291660/1‑148 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   CTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGCGGTCCTACTGCACAGTAAGTCTCTGACAG‑AAATCGGCTAAC‑ACCGGTTTAA‑TTGCCC‑GACGCCCTCTGGATTTTC‑CGAGGGCGTA‑TTTGTTTCTATGTCCTTC‑TTG‑‑CCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCC‑TGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATAT‑GTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACT‑CTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGG  >  NC_000913/3499470‑3499912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑