Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	345,302	C→A	17.2%	intergenic (+311/‑102)	yahL → / → yahM	uncharacterized protein/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,302	0	C	A	17.2%	188.0 / 3.9	73	intergenic (+311/‑102)	yahL/yahM	uncharacterized protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (46/13);  new base A (5/9);  total (51/22)
Fisher's exact test for biased strand distribution p-value = 3.64e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCAC  >  NC_000913/345063‑345523                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                ttttttttttgtttGCCACTTGGCTTTCCACTTTTTTTTTGCCCGATATTTTGTTGGGATCGGAG‑ACCCTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑G‑GGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GAc                                                                                                                                                                                                                                 <  1:1202164/230‑1 (MQ=255)    gagttctgtatGCCAACTGGCATTCCATCTCTTGTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAg                                                                                                                                                                                                                               <  2:1231343/235‑1 (MQ=255)          tttatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATTTTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTTGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTTACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAata                                                                                                                                                                                                                         <  2:784560/237‑1 (MQ=255)            tttggcAACCGTCATTCCAGCTTATTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACAATTCGCTGAAAAAAGCACCAGTATGGGTTTC‑GCAGCCATGCCGTGTATAAAAAATGATCTCATTCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑Tg                                                                                                                                                                                                                      <  1:798118/234‑1 (MQ=255)            tatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCCTGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑Tg                                                                                                                                                                                                                      >  2:663971/1‑239 (MQ=255)            tatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAata‑t                                                                                                                                                                                                                       >  2:109582/1‑238 (MQ=255)            tatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAata‑t                                                                                                                                                                                                                       >  2:1055832/1‑238 (MQ=255)            tatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑Tg                                                                                                                                                                                                                      <  2:352806/239‑1 (MQ=255)            tatGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACAAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGCGATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑TTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GCAGGAAAata‑t                                                                                                                                                                                                                       >  2:1059400/1‑238 (MQ=255)              ttccAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGa                                                                                                                                                                                                                              <  1:186734/228‑1 (MQ=255)              tGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑TTGGGGTCTTTTC‑GT‑AA‑TTTTCCGCATCGCACCAAAGCCT‑GACAGAAAAAA‑TGcg                                                                                                                                                                                                                    >  1:352806/1‑238 (MQ=255)              tGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGa                                                                                                                                                                                                                              >  2:186734/1‑230 (MQ=255)              tGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGGGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCAGGTTT‑GT‑GTGGGGCCTTTTC‑TG‑TA‑TCTTACGCATCGAACTCAAGCCT‑CACAGAAAAAA‑TGCt                                                                                                                                                                                                                    >  1:436389/1‑239 (MQ=255)              tGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGGTTATCAAAAATGATCTCATGCAGCTGTTTTGT‑GACTGAGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GGGGGGTCTTTTC‑GG‑TA‑TCTTACGCATCCCACTCAACCCC‑GAAAGAAAAAA‑Tgta                                                                                                                                                                                                                    >  1:1280758/1‑237 (MQ=255)              tGCCAACTGGCATTCCATCTCTTTTTCTGCAGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATACAAAATGATCTCATGCAGATGTTTTGT‑AAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GA‑GGTGGGTCTTTTC‑AG‑TC‑TCTTATGCCTCGCACTAAAGCAT‑GCAAGAAACTA‑Tgtt                                                                                                                                                                                                                    >  2:570650/1‑237 (MQ=255)                ccAACTGGCATTCCATCCTTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGt                                                                                                                                                                                                                  <  2:555196/239‑1 (MQ=255)                    cTGGCATTCCATCTTTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAata                                                                                                                                                                                                                         <  1:790654/229‑1 (MQ=255)                    cTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAata                                                                                                                                                                                                                         >  2:790654/1‑229 (MQ=255)                    cTGCCACTCAATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGAGGTTTC‑GCCGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAAACATAATTCCA‑TCACGTTT‑GG‑TTGGGGCCTTTTC‑TG‑TA‑ACTTAGCCATACCACTAAAACCT‑ACAAGaaaaaaagcgtaaga                                                                                                                                                                                                                >  1:849977/1‑227 (MQ=255)                                   ttttttCGCTTTTTTTTTTTTGTTAT‑GGCGCACACTTCGTTTAAAAAAGCACTAGT‑GGGGTTTCTCCAGCCATGCGGTGTATAAAAAATGATCTCTGGCATATGTTTTGT‑GAAGGTGTTGTTTGACTTTAATATGAAAAAAATCATAATTCCA‑TAATGTTT‑GT‑GTGGGGTTTTTTC‑TG‑TA‑TCTTCCGCACCGCACTCAAGCCT‑AACAAAAAATA‑TGTTGTAAGCCACATATCAAAAc‑cc                                                                                                                                                                                              <  1:1055832/239‑1 (MQ=255)                                     ttttCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCg                                                                                                                                                                                             >  2:702989/1‑238 (MQ=255)                                       ttCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCa                                                                                                                                                                                          >  2:1110004/1‑239 (MQ=255)                                                           aTCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCa                                                                                                                                                                      >  2:861301/1‑239 (MQ=255)                                                           aTCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GAAAGAAAATA‑TGCTGTACGGCTCATATCAAAAA‑CCGCCATTAGCTCATCTGGCAGAGCa                                                                                                                                                                      >  2:836255/1‑239 (MQ=255)                                                                     tctccgataaaaaaaGCCCCAGTTGTGGTTTC‑GCAGCAATGCGGTTAAAAAAAAATGATCTCATGCAG‑TTTTTTGTGGAATGTGTTGTTTGACATTAATATAAAAAAATTAATAATCCCA‑TCATGTTT‑GG‑TGGGGGTTTTTTC‑TG‑TA‑TTTTACGCACCGCACTCAAGCCT‑AACAAAAAATA‑GGTTGTAAGGTTAAAAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAAc                                                                                                                                                             <  2:487286/230‑1 (MQ=255)                                                                         cGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GCCAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAagag                                                                                                                                                                        >  2:288317/1‑224 (MQ=255)                                                                         cGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAgg                                                                                                                                                         >  2:494063/1‑239 (MQ=255)                                                                          gataaaaaaaGCACAGGTGGTGTTTTC‑GCAGCAGGGCGGTGAAAAAAAAATAATCTCAGGAAGGTTTTTTGG‑AATTTTGTGGGTTGACATTCATAAAAAAAAAATCATAATCCAA‑TCATGTTT‑GG‑GTGGGTTTTTTTC‑TT‑TA‑TTTTCCGCATCGCACTAAACCCT‑GAAAAAAAATT‑TGCTGTAAGGTTAAAAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGa                                                                                                                                                        <  2:1091340/235‑1 (MQ=255)                                                                                     cACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                                                                             >  2:121147/1‑239 (MQ=255)                                                                                     cACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGACGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGAATgg                                                                                                                                             >  2:1000481/1‑239 (MQ=255)                                                                                                   tC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAg                                                                                                                                >  2:28941/1‑238 (MQ=255)                                                                                                                            aTGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAACTCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑ACTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTACGACTCATATCATAAA‑CCGCCATTAGCCCAACAGGAAGAGCAAACGACAACCAGAATTGTTTTATGGTACGGGGTCAGCGGACCCGCTGGCGGGCCCACTTTTCa                                                                                                       >  1:791503/1‑239 (MQ=255)                                                                                                                            aTGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCa                                                                                                       >  2:781792/1‑239 (MQ=255)                                                                                                                            aTGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑ACGCCATTAGCTCATCAGGAAGAGCAGAGAACAAACAGGATTATTCAATGGTACGGAGTTGGAGACCTCGGAGGGGGCACAACttttgg                                                                                                       >  1:561249/1‑237 (MQ=255)                                                                                                                              gATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaa                                                                                                     >  2:1212210/1‑239 (MQ=255)                                                                                                                              gATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaa                                                                                                     >  2:660982/1‑239 (MQ=255)                                                                                                                              gATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCATCGATGGCGGGCCAACTTTTCaaa                                                                                                     >  2:634772/1‑239 (MQ=255)                                                                                                                              gATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGAACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaa                                                                                                     >  2:745367/1‑239 (MQ=255)                                                                                                                              gATCTCATGCAGATGTTTTGT‑GAATGTGCTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCCCTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGAATGGTATGGGGTTCGAGGCCGCGATGGACGCACAACTTTTCaaa                                                                                                     >  1:67209/1‑239 (MQ=255)                                                                                                                               acctttgggagtttttttGG‑GAATGGGTGGGTTGACATTCAAATAAAAAAAATCATAACCCCA‑TC‑TGTTTGGT‑GGGGGGTTTTTCC‑TG‑TA‑TCGTACGAAGCCCACCAAATCCA‑TAAAAAAAATT‑TGCTGTAGGGCTAAAAACAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                                                                    <  2:567879/225‑1 (MQ=255)                                                                                                                                    aggaagttGTTTGGT‑AAATGGTTTGGTTGACATTAATATGAAAAAAATCATAATTCCA‑TATTGTTG‑GT‑GTGGTGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GAAAAAAAATA‑TGCGGTAAGGCTAATATCAAAAC‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtctt                                                                                               <  2:149535/232‑1 (MQ=255)                                                                                                                                          aTGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTAGGGAACGGGGTTCGAGGCCTCGATGCAGGTCCAACTTTTCAAAACTCTTCTGaa                                                                                          >  2:1223663/1‑238 (MQ=255)                                                                                                                                          aTGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTAC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATCTCACAAC‑CCGCCATTAGCTCATCAGGTAGAGCAGACGCAAACCAGGATAGTTGTATGGAACGGGCTTGGAGGACTCGATGGCGGTCCCACTTTTTAAAACACTTCTGAAt                                                                                         >  1:567879/1‑239 (MQ=255)                                                                                                                                              tgtgt‑gAAGTTGTGGTTTCACATTAGAATAAAAAAACTCAT‑ATACCACTTATGTGT‑GT‑GGGGGGTCTTTCC‑AG‑TA‑TCGTACGCACCTCACTCAAGCCT‑GACAGAAAATA‑TGGTGTAAGGCCAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTACAAAACTCTTCTGAATCAGa                                                                                     <  2:78058/237‑1 (MQ=255)                                                                                                                                                  ttaaaTGTGGTGGTTATAATTAATAAAAAAAAAATAAT‑ATTCCA‑TTTTTGTT‑GT‑TGGGGGTTTTTTT‑TGTTT‑TTTTCCGCCTCGCACTCAACCCA‑AAAAAAAAATA‑TGCTGAAAGCCAAAAAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcc                                                                                  <  2:1249930/236‑1 (MQ=255)                                                                                                                                                     aaTGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCATGAAGAGCAGACGTCAACCAGGATTGATGTATGGTACGGGGATCGAAGCCCAGATGGCGGCCCAACTTTTAaaaaactatcgtaaacaggaacacc                                                                               >  1:638031/1‑218 (MQ=255)                                                                                                                                                     aaTGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGCATCAGATCCCCt                                                                               >  2:1144980/1‑239 (MQ=255)                                                                                                                                                     aaTGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGAGGGCGGCCCAACTTTTCAAAAACATTCTGAATAAGATCCCCt                                                                               >  1:459055/1‑239 (MQ=255)                                                                                                                                                           tttgttctattttttataAAAAAAACTATAACCCCA‑TCTGGTTT‑G‑‑GGGGGGTTTTTTCTTG‑TA‑TCTGACACA‑CCAACACAAACCTAGAAAAAAAATA‑TGAGAAAGGGCACAAATAAAAAT‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                                                                         <  1:660982/225‑1 (MQ=255)                                                                                                                                                               tggactttaaaaaaaaaaaaaTATTATGTCCA‑TCGTGTTG‑GT‑GGGGGTTCTTTTC‑TG‑TA‑TCTTCCGCCCCGCACTCAAGCCG‑AAAAAAAAATT‑TGTTGTGAGGCCAATATAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcat                                                                      <  2:776416/224‑1 (MQ=255)                                                                                                                                                                             aaaaaaaTCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTTTTGTATGGTACGGGGTTCGAGGTCTCGACGGCGGCCCAACTTTTCAAAAATATTCTGAATACGATCCCCTTATTCTCATCCCTGCAAAGGGGa                                                        >  2:933934/1‑238 (MQ=255)                                                                                                                                                                             aaaaaaaTCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATACCTGCAAAGTGGaa                                                       >  2:1240350/1‑239 (MQ=255)                                                                                                                                                                             aaaaaaaTAAAAATCCCA‑TGTTTTTG‑GG‑GGGGGTTTTTTTA‑TG‑TA‑TCTCACGCATCGCACACAAGCCT‑GACAAAAAATA‑GGCTGAAGGGCTAATAACAAAGC‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGaa                                                       <  2:496371/239‑1 (MQ=255)                                                                                                                                                                               aaaaaTCATAATTCCA‑TCATGTTT‑GG‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGCCGACAACCAGGATTGTTGAATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTTTTATCATCCCTGCAAAGTGGcaca                                                     >  2:1254052/1‑239 (MQ=255)                                                                                                                                                                                         aTTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGTCGGTCCAACTTTTCAAAACTCTTTTGATTCAGATCCCCTTTTTATCATTCCTGCAAAAGGGAAAAtttctata                                             >  1:496371/1‑231 (MQ=255)                                                                                                                                                                                              tatatgtttt‑GG‑GTGGGTTTTTTTC‑GT‑TA‑TCTAAAGCATCGCACTCAAGCCT‑ACAAGAAAATA‑GGCGTGAAGCTTCTTTATAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCTACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTgg                                       <  2:397666/232‑1 (MQ=255)                                                                                                                                                                                                tcatGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCt                                     >  1:129795/1‑239 (MQ=255)                                                                                                                                                                                                     ttt‑GTGGTGGGGTGTTTTC‑TG‑TATTCTCCCGTA‑CGCACCCAAGCCC‑GAAAAAAAATATTGGTG‑AAGGCCTAAATAAAAAATCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTAtt                                  <  2:1280758/239‑1 (MQ=255)                                                                                                                                                                                                       t‑gt‑gtgGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTACCAACTCTTCTGATTCAGATCCCCTTATTATCATCCCTCCAAAGTGGAAACTTGCCacatgacccttatacacc                              >  1:591606/1‑222 (MQ=255)                                                                                                                                                                                                         gt‑gtgGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTTTTTGGCTATTCAGGa                             >  2:854768/1‑239 (MQ=255)                                                                                                                                                                                                         gt‑gggGGGTTTTTTC‑TT‑TA‑TCATACGCATCGACCTAAAGCTT‑AAAAAAAATGT‑TGCTTAAAGTCTAATAAAAAAAG‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGa                             <  2:436389/239‑1 (MQ=255)                                                                                                                                                                                                               gggTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATgggg                        >  2:335390/1‑239 (MQ=255)                                                                                                                                                                                                                ggTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCACTTGTTTGGCTATTAAGGATGGGGc                       >  1:13173/1‑239 (MQ=255)                                                                                                                                                                                                                    ttttC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGAGGGGGCAGa                    >  2:994348/1‑238 (MQ=255)                                                                                                                                                                                                                     tttC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGGTCGAGGCCTCGAGGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTTCAAAGAGGCACATTTCAATTGTTTGGCTATTCAGAATGGGGCAGAca                  >  1:1296015/1‑238 (MQ=255)                                                                                                                                                                                                                     tttC‑TG‑TA‑TCTTACGCATCGCACTCAAACCT‑GACAGAAAATA‑TTCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCACCAGGAATAGCAGACGACAACCAGGCTTGTTGTCTGGTACGGGGTTCGCAACATCGATGGCGGTCCACCTTTTCAAAACTCTTCTGAATACGATCCCCTTCTTATCATCCCCGCACCGTGTAACCTTGCCATTGTTTgctgtttccggagagggacgacc                  >  1:979040/1‑217 (MQ=255)                                                                                                                                                                                                                     tttC‑TG‑TA‑ACTTACGCATCGCTCTCAGGCCT‑GACAGAAAATA‑TGCTGTAAAGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGAAGACGACAAACAGGATTGTTGTGTGGTACAGGGTTCCAGTGCTCGTAGACGCACCAACCATTGAAAACACTCCGCAGTCAGATCCCCTCATTTTCATCCCTGCAACCAGGAACCTTGCAATGGATTGGCTATTACGGATGGGGCAGAc                   >  1:657936/1‑238 (MQ=255)                                                                                                                                                                                                                              a‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTCCGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGa                                                                                     >  2:752703/1‑165 (MQ=255)                                                                                                                                                                                                                                tCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑Tg                                                                                                                                                                                                                      <  2:740202/36‑1 (MQ=255)                                                                                                                                                                                                                                   tACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTCCAAAGTGGACCATTGCCCTTGTTTTGCCCTTCAGGTATGGGCAAACCAAGAaaaaaa        >  1:874407/1‑238 (MQ=255)                                                                                                                                                                                                                                        acccgacttcaaaccataCAAAAAATA‑TTGTGAAAGGCTAAAAAAAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTAGGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCca   <  2:791503/222‑1 (MQ=255)                                                                                                                                                                                                                                        aTCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGa                             >  2:201823/1‑212 (MQ=255)                                                                                                                                                                                                                                        aTCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATAAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCCATTCAGGATGGGGCAGACCAGTGAAAAAAGACcac  >  2:525790/1‑239 (MQ=255)                                                                                                                                                                                                                                          cGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCt                                                                                                                                                                                                            >  1:740202/1‑36 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                CTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCG‑ACACTTCGCTTAAAAAAGCACCAGTAGTGGTTTC‑GCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGT‑GAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCA‑TCATGTTT‑GT‑GTGGGGTCTTTTC‑TG‑TA‑TCTTACGCATCGCACTCAAGCCT‑GACAGAAAATA‑TGCTGTAAGGCTCATATCAAAAA‑CCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCAC  >  NC_000913/345063‑345523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑