Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,762,268 T→G 22.8% G29G (GGT→GGG rhsA → Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,762,2680TG22.8% 71.1 / 2.2 60G29G (GGT→GGGrhsARhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (18/25);  new base G (12/4);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAAATAAATCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTG  >  NC_000913/3762036‑3762499
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
tCCAAATAAATCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGCCAGGGCGACATGACGCAGTATTGCGGACACATTGTTCAGGGTTAAGCCGGGGGGGGCCTTGGttcgcc                                                                                                                                                                                                                                             >  2:1272961/1‑233 (MQ=18)
 tttaagagacaacaTTTTTTTCATGGTGATATAAATATTTTCCTAATTATTTCACTTTGATTGATAACACACTTCAGGGTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCa                                                                                                                                                                                                                                           <  1:1032526/228‑1 (MQ=17)
         atCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGACTTGTTCCGGGTTCAGCCGGGGTGCGCCTTTTGGCCCCCACCGGCGTg                                                                                                                                                                                                                                  >  2:461655/1‑239 (MQ=255)
                        tGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTTACCCGGGGTGCGCATTGGGGCCCCCACCGGCGGGGGCCTGtacctttt                                                                                                                                                                                                                    >  2:1032526/1‑231 (MQ=17)
                        tGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTGTTCAGGGGTCAGCCGGGGGGCGCATTGGTGCCCCCACCGGCGGTGCCCGTTCgggggg                                                                                                                                                                                                                  >  2:738394/1‑234 (MQ=255)
                                    tagtttCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGc                                                                                                                                                                                                                                                <  2:1040173/196‑1 (MQ=255)
                                     atTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAATGTTCCGGGGTCAGCCCGGGGCCGCGCTTGGGCTCCCCCCCGGCGTGCCTGTTccggggggcaccgaggagggg                                                                                                                                                                                                     >  1:896026/1‑219 (MQ=17)
                                        ttCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGAACTGAGGCAGTATGACGGCAGGACATTTCAGGGGTCAGCGCGGGCGCGCATTGGGGTCCCCACCGGCGTCGCCTTTTCGCGTAGGCCCcggggaggggtgg                                                                                                                                                                                                >  1:872472/1‑229 (MQ=17)
                                           cTAATTTTTTCCCGTTGTTGGATATCCCCCTTTAGGGTTTCTTATAAATTTGTAGGGTTTCGCCGGTCAGACGAAAAATAACCCGATAAAACAAGGAAGAGAAACGAGCGGAAAACCGGCAGCGGGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCCCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCg                                                                                                                                                                                           <  1:461655/239‑1 (MQ=14)
                                                       gtgtgtacaagagacaggttCAGGCTTTCTTAAAAATTTTTAGGGGTACGCCGGGGAGCAGACAAATAACCCGATAAAACAAGGAAGAGAAAGGGGCGGAAAACCGGCAGCTCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCa                                                                                                                                                                               <  1:277470/221‑1 (MQ=14)
                                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGGGCATTTTGGCCCCCACCGGCGTGGCCCTTTCGGGGTGCCCCGGCGGGGTGAAGGCCGGCCCTCCGGTCAAAcc                                                                                                                                                                           >  2:996050/1‑238 (MQ=14)
                                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGCGCATTGGTGCCCCCACCCGGCTGGCCCGTTCGGGTTGCCCCGGCGGGGGGGCCGCCGGCCCTCCCGGCAATcc                                                                                                                                                                           >  2:677428/1‑238 (MQ=14)
                                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCCGGGGGCGCATTGGGGCCCCCACCGGCGTGGCCTGTTTGGGGGGTCCCGGCGGGGTGACGTCCGGCCACCCCGTCAATcc                                                                                                                                                                           >  2:238004/1‑238 (MQ=14)
                                                              tgaagtcatgtttgaGGTTTTATTAAAAATCTGGGGGGTGTCGCCAGTACGTCGACCAATAGCCAGATAAAACAAGGATGAGAAATGTGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTAGGGCGGTAGCATTGTGCAGGGTTCAGCCTGGGTGTGCATTGGTACCCCCCCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCt                                                                                                                                                                        <  1:174620/225‑1 (MQ=14)
                                                                  ttcttccTTCAGGTTTTTTTATAAATCTGTAGGGTTTCGCCTGGCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGTCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCt                                                                                                                                                                     <  1:238004/232‑1 (MQ=255)
                                                                  atgtgtataagaggcagcTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCt                                                                                                                                                                     <  2:143372/221‑1 (MQ=255)
                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGAGGCAGTATGGCGGTAGCATTGTTCCGGGTTCAGCCGGGGGGGGCATTGAGGGCCCCCCCCGGGTGGGCTGTTGGGTGGGGCCCCGGGGGGGGACggccggccccccgcggcatcccccccccgg                                                                                                                                                                  >  1:975244/1‑217 (MQ=14)
                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGCCGGGGGGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTTGGGTTGCCCCGGCGGGGGGACGGCCGGCCCACCCGGCAAACCCCCGccccg                                                                                                                                                                  >  2:991085/1‑235 (MQ=14)
                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCACTGTTAAGGGGTCAGCCGGGGGGAGACTTGTGGCCCCCCCCCGCGCGGCCTGTTTGGGGGTGCCCCGCGGAGGGGCGGCCCGcccccccggccaaccccccccccg                                                                                                                                                                  >  1:1202354/1‑217 (MQ=14)
                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAACAAGGTTCCGGGGTCAAGCGGGGGGGGCCCTGGTGGCCCCACCCGCGGGGGCTGTTCGGTGGGCCCCGACGGTGGGGCGCCCGGCCCCCCGGGGCATCCCCCGCCCgg                                                                                                                                                                 >  1:363002/1‑239 (MQ=14)
                                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTGTTCAGGGGTCAGCCGGGGTGCGCCTTTGGGCCCCCCCCGGCGTGGCCTGTTCTGGGTGCCCCGGCGGAGTGACGTCCGGCCACCCGGGCAATCCCCTGCTCgg                                                                                                                                                                 >  2:1272505/1‑239 (MQ=14)
                                                                        cTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCa                                                                                                                                                           <  2:975244/239‑1 (MQ=255)
                                                                                   cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCCTTGTTCAGGGTTCAGCCGGGTTGCGCCTTTGTGCCCCCACCGGCGTGGCCTGTTTGGTGTGCCCCCGCGGGAGGACGGCCGGCCCTCCGGGCCATCCCCCGCCCGGGGCCAAGGACCTTc                                                                                                                                                 >  2:8589/1‑238 (MQ=14)
                                                                                   cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGCGCCTTGGTGCCCCCACCGGCGTGGCCCGTTCGGTGTGCCCCGGCGGGGTGAAGTCCGGCCCTCCGGGCAATCCCCCGCTCCGTGCCAAAGGGCCtc                                                                                                                                                  >  2:773840/1‑237 (MQ=14)
                                                                                   cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAATGTTCAGGGGTCAGCCGGGGGGGGCATTGGTGCCCCCCCCCGCGTGGCCGGTTCGGTGTGCCCCGGCGGAGGGACGTCCGGCCATCCGGGCAAACCCCCGCt                                                                                                                                                                     >  2:277470/1‑221 (MQ=14)
                                                                                            ctttAGGTTTTCGCCGGTCAGCAGACAAAGAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGGCGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAAc                                                                                                                                       <  1:738394/236‑1 (MQ=255)
                                                                                             tGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCACTGTTCAAGGTTCCGCCGGGGGTCGCATTGTGGGCCCCCCCGCCGGGGGCCGGTCGGGGGGGCCCCGCGGGGGGACAGCCGGCCCCCCCGGCAATCCCCCGCCCGGGTGCCAAGTGCCTCCCGGGGaaaac                                                                                                                                      >  1:119295/1‑237 (MQ=2)
                                                                                              gTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAACGCGTCAGGGCGACATGACGCAGTATGGCGGGAACAATGTTTAGGGGTCAGCCGGGGTGCGCATTTGGGCCCCCCCCGGCGGGGCCTGTTTCGCGGGGCCCCGCGGGCTTGACGCCCTGCCCACCGGCAACCCCCCCCCCGGGGCGAAAGGCCCCTCCCGGGGAAAc                                                                                                                                       >  1:513032/1‑238 (MQ=9)
                                                                                                      tCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCCGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGGGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGGCCTTTCCGGCGAAACCGACCTCGc                                                                                                                              >  2:36113/1‑238 (MQ=255)
                                                                                                      tCGCCTGGCAGCAAGCAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGcc                                                                                                                             <  2:872472/239‑1 (MQ=255)
                                                                                                         ccTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTTAAATTTTTTAGGGGTTAGCCGAGAGGCGCAACGTGGCCCCCACCACGGCGGCCCGTTCCTTGGTGCCCGCGCGGGGGGGGCCCGGCCACGCAGGCACTCCCCTCCCCGGTGCGAAGGGCGTTccctgcggaccccacaacccccc                                                                                                                           >  1:308182/1‑218 (MQ=2)
                                                                                                         ccTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGGATTGTTCAAGGGTCACCCCGGGGGCGCCTTGGTGCCCCCAACGGCGGGGCCTGATATGGGGGCCCCCGCGGGGGGTAGGCCGGCCATCCCGGACATCCCCGCCTCGGGGCCCAGGGGCTTTCCCGGGAAACCCACACCCCCCt                                                                                                                           >  1:854566/1‑238 (MQ=9)
                                                                                                                 cagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGGATTGTTCAGGGGTCAACCGGGGGGGGCAATGGTGGCCCCACCGGCGGGGCCCGGTGGGGGGTCGCCGGCGGGGGGGCGTGCCGGCCACCCGGCAACACCCCGCCCCGGGCCAAGGGCCCTCCCCCGGAAACCCCCATCcccccccccccgcg                                                                                                                  >  1:1249435/1‑229 (MQ=2)
                                                                                                                       aaTAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAACATTGGTCCGGGTTCAGCCGGGGGGCGCATTGGTGCGCCCACCCGCGTGGCCCGTTTGGTGTGGCCCCGGCGGGGGACGGCCGGCCCCCCGGCCACTCCCCTGGTCGCGGCCAACGGCCTTCCCCGGGAAACCCACACCCCCCCcccccgccccgcccc                                                                                                            >  1:144063/1‑233 (MQ=9)
                                                                                                                         tAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGt                                                                                                          <  2:101870/239‑1 (MQ=255)
                                                                                                                                aTAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGGACGTCCGGCCATCCGGTCAATCCCCTGCCCGGGGCCAAGGTCCTTCCCGGTGAAACCGACATCGCCCTCCCCGGCCCCCTCCCGTTCATcc                                                                                                    >  2:642777/1‑238 (MQ=14)
                                                                                                                                     aCAAGGATGAGAAATGAGCGGAAATCCGGCAGCGCGTCAGGGCCACATGGCGCCGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTccc                                                                                              <  2:939222/239‑1 (MQ=255)
                                                                                                                                     aCAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCGGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTccc                                                                                              <  2:854566/239‑1 (MQ=255)
                                                                                                                                            tGAGAAATGGGCGGAAACCCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  2:1202354/239‑1 (MQ=255)
                                                                                                                                             tggaAATGGGGCGAAAACCGGCGGCGCGTCAGGGGGGCGTTGCGGGGTGTGGCGGTAGCCTTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  1:642777/236‑1 (MQ=11)
                                                                                                                                             gggaAATGGGCGGAAAACCGGCGGCGGGTCGGGGGGACATGCCGCAGTAGGGCGGTGGCATTGTTCAGGGTTCAGCCGGGGGGCGCATTGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  1:1272505/236‑1 (MQ=11)
                                                                                                                                             gaggAATGAGCGGAAAACCGGCAGCGCGTAAGGGCGACAGGACGGAGTTTGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTa                                                                                       <  2:571900/238‑1 (MQ=255)
                                                                                                                                                 aaTGGGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGCGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTAcagc                                                                                   <  2:119295/238‑1 (MQ=25)
                                                                                                                                                                     cgcgTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGcc                                                               <  2:582885/238‑1 (MQ=33)
                                                                                                                                                                        gTCAGGGCGACATTCCTCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCcgcg                                                           <  2:477454/239‑1 (MQ=18)
                                                                                                                                                                                 aaaTGACGCAGTATGGCGGTAGCATTGTTCGGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTggg                                                   <  2:129534/236‑1 (MQ=17)
                                                                                                                                                                                  cATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTgggg                                                  <  2:248647/238‑1 (MQ=32)
                                                                                                                                                                                      aCGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATGGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGGTACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGcc                                              <  2:886311/238‑1 (MQ=17)
                                                                                                                                                                                           gTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGcc                                        <  2:1277338/239‑1 (MQ=32)
                                                                                                                                                                                                          tGTTCAGGGTTCAGCCGGGGTGCGCATTGGGGCCCCCCCCGGCGGTGCCTGGTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCCGCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGc                         <  2:144063/239‑1 (MQ=255)
                                                                                                                                                                                                                 ggTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCCACTACAGCAGGTACCGGCCAAAAACGCCCGCGCCGGTGGGGAGGCTCGGCCCCCGCCGGAAAAAGCCAGCGGa                  >  1:62470/1‑239 (MQ=11)
                                                                                                                                                                                                                    tCAGCCGGGGTGCGCATTGGGGCCCCCCCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat               <  2:497566/239‑1 (MQ=17)
                                                                                                                                                                                                                     cAGGCGGGGTGCGCATTGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat               <  2:1031017/238‑1 (MQ=17)
                                                                                                                                                                                                                     cAGCCGGGGTGCGCATTGGTGCCCCCCCCGGGGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGatat               <  2:707827/238‑1 (MQ=17)
                                                                                                                                                                                                                      cggcGGGGAGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGCCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCCGTGCAAAGGGCCTTCCCGGTGAAACCGACATCCCCCGGCCCGGCCCGCTGCCGTTCATCCTCCCCCGCACCTACACCAGTTAACGGGCAAAACCACCCGCGCCGGCGGGGGGCCTGCGCCCCGGCCGGcaaaaacacgcggccagcc             >  1:18147/4‑224 (MQ=11)
                                                                                                                                                                                                                      aGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACCAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCGGGGAAATGCCTGCGGACATcc             >  2:551815/1‑239 (MQ=12)
                                                                                                                                                                                                                           gggTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCCCCCCCACCCACCGCCGTTACCGGCCAAAAACGCCCGCGCCGGCTGGGGGCCCCGCCCCCGCCCGGCAAAtacaccggcacacacgccg         >  1:1211424/1‑220 (MQ=11)
                                                                                                                                                                                                                           gggTGCGCATTGGTGCCCCCACAGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCCTCCGGCCAATTCCCTGCTCGGGGCAAAGGTCCTTACCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCCGTTACCGGCAAAAAACGCCCGGCCCCGCGGGGGGCTCCGGCCCCCGTCGGaaaaaagcaccggagaacccctgc        >  1:101793/1‑219 (MQ=11)
                                                                                                                                                                                                                             gTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGCCAAAAACGCCCGCCCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTCCGGATATCCGCTTAc       >  2:580260/1‑238 (MQ=17)
                                                                                                                                                                                                                                 gcATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg  <  2:363002/239‑1 (MQ=11)
                                                                                                                                                                                                                                  cATTGGTGCCCCCACCGGCGGGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTg  <  2:36264/238‑1 (MQ=11)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
TCCAAATAAATCACATATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTG  >  NC_000913/3762036‑3762499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: