BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,901,864	A→C	9.8%	V119G (GTG→GGG)	bglH ←	carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,901,864	0	A	C	9.8%	339.2 / 3.5	136	V119G (GTG→GGG)	bglH	carbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand): ref base A (46/72); new base C (8/9); total (54/82)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

ACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAC  >  NC_000913/3901631‑3902100

cagaGCCGGTAGAGTACCTCTGACCAACTTTACCATCCATCATCCCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACaaa                                                                                                                                                                                                                                           <  2:665034/235‑1 (MQ=255)

    gCCGGTAGAGTTCTGCTGACCAACGGTACCATCCATCATCAAAACGGCATCAACCAGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTGAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑c                                                                                                                                                                                                                                     <  2:574276/239‑1 (MQ=255)

        gtcaggttctgctcACCAATGGTACCATCCAACATCACAACGGCATCAACCCGTTTGCCTTTTCCGTTGTAGACAC‑GTTGTTTCAACTGCAAACCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGaa                                                                                                                                                                                                                                 <  1:960144/231‑1 (MQ=255)

        ggtgtgTATTGCTGACCCACTGTCCCATCCATCATCACAAGGGCATAAACCCGTTTGCCGGTTTCGTTGTAGAACC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGaa                                                                                                                                                                                                                                 <  1:915859/234‑1 (MQ=255)

         tAGAGTCCTGGTGACCAACGTTACCATCAATCAACACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGaa                                                                                                                                                                                                                                 <  2:491707/238‑1 (MQ=255)

                ctgctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCa                                                                                                                                                                                                                         <  2:177651/239‑1 (MQ=255)

                 tgtttaCCATCGTTACCATCCAATCTCACAACGGCATCAACCCGTTGGCCGTTTGCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGTC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCag                                                                                                                                                                                                                        <  1:814290/234‑1 (MQ=255)

                   ctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCCGAAC‑GGTATAAGCCGTTGTAATTAAA‑ACCAATTTCATCTTTCACAACTTTA‑ATGAAATCTTTCagag                                                                                                                                                                                                                      >  2:1163118/1‑239 (MQ=255)

                   ctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAACCGTCCCAGAGAACCAATAGCCCATGATTTAGGTGAACCATGAAAGAC‑CGGCCCCCAA‑CCAGAACGGGCAATAGCCGTTGTACAAAAG‑GACAAATACA‑CTTTCACAAAATTA‑ATaaaaaactttaaag                                                                                                                                                                                                                      >  1:359652/1‑229 (MQ=255)

                        cAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCCGAAC‑GGAAATAGCCGTTGTAACTAAA‑ACCAATTTCATCTTTCACCAATTTC‑CTGAAATCTTTCAAGGTCATg                                                                                                                                                                                                                 >  2:1279102/1‑239 (MQ=255)

                        cAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGCC‑GGTCCCCCAA‑CCCAAAC‑GGGAAACACCCTGGTAAATAAA‑GCCAATTTCACCCTTCCCAAATTTACCTAAAACTTTTCagagaacg                                                                                                                                                                                                                  >  1:1325577/1‑235 (MQ=255)

                          cccttcccctccatcCTCCAAACCACATCACCCCCTGTGCCCTTTACGGTTTACACTC‑GTTGGTGTAACTGCAAATAAAACCAGCCTGAGTATTCGTTACCAAAGCGGCCCAGAGCACCAAGCGCCCATGATGTAGGTGAACCAGAAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTGCAGAGTCATGga                                                                                                                                                                                                               <  1:44537/230‑1 (MQ=255)

                           cGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGGAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGgaa                                                                                                                                                                                                              >  2:431399/1‑239 (MQ=255)

                             ttACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAGATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGCACCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAACACCAATTCCA‑CTTTACCAAATTAA‑CTAAAAACTTTCaaaaacaaggaaaa                                                                                                                                                                                                            >  1:509427/1‑226 (MQ=255)

                             ttACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGGAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTC‑ATGAAATCTTTCAGGGTCATGgaaga                                                                                                                                                                                                            >  2:178710/1‑239 (MQ=255)

                              tACCATCCATCTTCACAACGGCACCAACCCGTTGGCCGTTTTCCTTGTAGACCC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGgaagaa                                                                                                                                                                                                           <  2:470945/239‑1 (MQ=255)

                                   cccactctcaCAACGTCACCAACCCTTTGGCCGTTTTTGTTGTAG‑CACATTTGTTTTAACTGCAAATCAAACCAACCGGAGTATTCGGTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTAAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCTGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAAt                                                                                                                                                                                                      <  1:1163118/232‑1 (MQ=255)

                                     catcatCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGCGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCCCAAAATTA‑CTGAAATCTTTTCGAAGACTGGAAGAATAAATCg                                                                                                                                                                                                    >  2:821577/1‑239 (MQ=255)

                                     catcatCACAACGGCATCAACCCGTTTGCAGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAACCGGCCCAGAGAACCAAACGCCCATGATTTAGTAGAACCATGAGAGGC‑GGTCCCCCAACCCAAAAC‑GG‑AATAAGCGGTGAAAAGAAA‑GCCAATTCTACCTTCTAAAAATTTA‑CATAAATCTTTTAGAGGAATGGTaaaaaaaaaaa                                                                                                                                                                                                    >  1:326799/1‑236 (MQ=255)

                                      aatatcaCAACGGCCTTCACCCTGTTGCCGTTTTTGTTGTAGCCCC‑GGTGTTTTAACTGCAAATAAAACCGGCCGGAGTATTCGTAACCAAAGCGGCCCAGACACCCAATCGCCCAGGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCAAATTTCATCTTTCACAAATTTA‑CTGAAAGCTTTCAGAGTCATGGAAGAATACATCGa                                                                                                                                                                                                   <  1:622192/236‑1 (MQ=255)

                                        catccaagcggatccaccccGTTTCCCATGTCGGTTGGACACAC‑GTAGTTTGAACTGCAAATAAACCCAGCCGTGCTATTCGTTGCCAAAGCGGCCCAGAGAACCAATCGCCAATGGTTTAGGTGAACTATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAT‑GCCAATTTCATCTTTCCCAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGc                                                                                                                                                                                                 <  1:1247334/222‑1 (MQ=255)

                                           cacaACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCCA‑CCCGACC‑GCGAATAACCGGTGTAACTCAA‑ACCAATTTCATCTTTCACAAATTTA‑ATGAAAACTTTTAGAGTCATGGAaaaaaaaaacaaaacc                                                                                                                                                                                               >  1:429203/1‑230 (MQ=255)

                                                 ggCCTCAACCCGTTTCCCGTTTTCGTTGTAGCACC‑GTTGTTTACACTGCAAATCAAACCGGCCGAAGTATTCGTTCCCAAAGCGGCCCAGAGAACCAAGCGCCCATGATTTAGGTTAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCTTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCAc                                                                                                                                                                                        <  1:387156/239‑1 (MQ=255)

                                                     tCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGCGAACCAATCGACCATGCTTTAGGATAACCATGAGAGCC‑GGTACCCCAA‑ACAGAAC‑GGAAATAACCGTTGTAACTAAA‑GCACATTTCACCTTTCACAAATTTA‑CTGCAAGCTTTCAACGTTCAGGAAAAATAAAACGAACCTTTCCCCCTGGc                                                                                                                                                                                    >  1:500458/1‑239 (MQ=255)

                                                        aCCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCCAAAC‑GGTAAAAACCCGTGGAACTAAA‑GCCAATTTTCCCTTTCAACAATTTA‑CTGAAATTTTTTCAAGTCATGGGAAAAAAAAACGCACCTGtccccacggcatt                                                                                                                                                                                 >  1:717468/1‑228 (MQ=255)

                                                        aCCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CACGAAC‑CAGAATATACGTCGTATCTACA‑GCCAATTTCATCTTTCACAAAATTA‑CAGAAATCTTTCTGAGTCAATGAAAAATAAAACAAGCATGTCCCAATgcaatt                                                                                                                                                                                 >  1:309094/1‑234 (MQ=255)

                                                               tGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCCGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAAGGTCATGGGAGAATAAATCGAACCTGTCTCACTGGCATTTTTttct                                                                                                                                                                          >  2:1040393/1‑239 (MQ=255)

                                                                gcctttttgtttttggACAC‑TTTTTTTTAACTGAAAATCAAACCAGCCGGAGTATGCTTTCCCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCtt                                                                                                                                                                         <  1:1040393/224‑1 (MQ=255)

                                                                gCCTTTTTTGTTTTAGACAT‑GTTTTTTTAACTGAAAAACAAACCAGCCGGAGTTATCGGTACCAAAGGGGCCCAGGGAACAAATCGCCCATGATTTAGGGGAACCATGAGAGGC‑GGTCCCCCAC‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCtt                                                                                                                                                                         <  1:350758/239‑1 (MQ=255)

                                                                 ccGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAGGCTGCCCAGAGAACCAATCGCCCATGATTTAGGGGAACCATGAGAGGCGGGCCCCCCCA‑CCA‑AAC‑GGTAATAACCGGTTGAACTTAA‑ACCAATTTCCACTTTCCCAAATTTT‑ATCACATTTTTCAGAGTCATGGAAAAAAAAATTCCGCCTTTCTCCCTGGCCTTTTTCttttt                                                                                                                                                                        >  1:1250032/1‑238 (MQ=255)

                                                                     ttttGTTTTAAACCC‑GTTGTTTTAACTGAAAATAAACCCAGCCGGAGTTTTCTTTACCAAAGGGGCCCAAATAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCAAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCAttt                                                                                                                                                                    <  1:178710/239‑1 (MQ=255)

                                                                     tctccttttagcccc‑GTTTTTTTAACGGAAAACTAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAAAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCAttt                                                                                                                                                                    <  2:1213741/225‑1 (MQ=255)

                                                                          tttttaacat‑GTTGTTTGAACGGCAAATCAAACCAGCCGGATTATTCTTTACCAAAGCGGCCCAGAGAACCAATCGCCCATTATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAG‑GCCAATTTCATCTTTCACAAATTTA‑TTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  1:511387/233‑1 (MQ=255)

                                                                          tttgcaacac‑GGTTTTTTACCAGAAAACCAACACCGCCGGAGTTTTGGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGAATTGGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CAAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATGTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  1:1070083/233‑1 (MQ=255)

                                                                          ttGTAGACAC‑GTTTTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGACCCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  2:532987/239‑1 (MQ=255)

                                                                          ttGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  2:542832/239‑1 (MQ=255)

                                                                          ttGGAGACAC‑GTTGGTTTAACGGCAAACCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATTATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCCCAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  2:601706/239‑1 (MQ=255)

                                                                           tttttatactgttgttTAAAATCAAAAACAACCCAGCCGGAGTATTTGTTCCCAAAGCGGCCCAGAAAACCAAGCGGCCATGATTTAGGTGAACCATGAAGGGC‑GGTCCCACAA‑CCAGAAG‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  1:1315275/229‑1 (MQ=255)

                                                                           tGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAcc                                                                                                                                                              <  2:13912/239‑1 (MQ=255)

                                                                           tGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  2:500458/238‑1 (MQ=255)

                                                                           tGTAGACAC‑GTTGTTTTAACGGAAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  2:880832/238‑1 (MQ=255)

                                                                           tGTAAACAA‑GTTGTTGTAACTGAAAACCAAACCAACCGGGGTATTCGTTACAAAAGCGGCCCAGGGAACCAATCACCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  2:1240347/238‑1 (MQ=255)

                                                                              taacaatgttgttTTAACTGAAAAACAAACCAGCCGGAGTATTCGGTACCAAGGCGGCCCAGAGAACCAAACGCCCATGATTTAGGTGAACCATGAGAGGC‑GTTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAg                                                                                                                                                            <  1:84313/232‑1 (MQ=255)

                                                                                 cac‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATGCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTa                                                                                                                                                          <  2:154877/237‑1 (MQ=255)

                                                                                     gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTTAAATCTTTCAGAGTCCTGGAAGAATAAATCGAGCCCGTCTCACTGGCATTTTTTTCTTCATTTTTGAACATGACAGCa                                                                                                                                                     >  2:1230287/1‑239 (MQ=255)

                                                                                     gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTTTCTTCATTTTTGACCAGTACACCa                                                                                                                                                     >  2:509993/1‑239 (MQ=255)

                                                                                             aaCTGAAAATCAAACCAGCCGGAGTATGCGTTACCAAAGCGGCCCAGAGACCCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGttt                                                                                                                                             <  2:509427/239‑1 (MQ=255)

                                                                                               tttcaatacAAACCAACCGGAGTATTCGGTACCAAATTGGCCCAGAGAACCAATCGCCCATGAATTAGTTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATTGCCGTTGTATCTAGA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTg                                                                                                                                           <  2:731704/231‑1 (MQ=255)

                                                                                               cTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGtttt                                                                                                                                            >  2:292625/1‑238 (MQ=255)

                                                                                                  cAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCCCAAATTTA‑CTGAAATCTTTCCGAGGCATGGAAAAATAAATCCAGCCTTTTTCACTGGCATTTTTCTCTTCATTTTTTACCAGTACAGCATCAGGGTTTGCg                                                                                                                                         >  2:579098/1‑238 (MQ=255)

                                                                                                  aaataccacccccacgaatttttCGTTCCCAAATGGGACCAGAGAACCAATCCCCCATGATTGAGGTGACACATGAGGGGA‑GGTCCCCAAA‑CCAGAAC‑GGTAATAGCCGTTGTTACTAAT‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAATAATAAATCGAGCCTGTCTCACTGCCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGATTTTGAgg                                                                                                                                        <  1:821577/218‑1 (MQ=255)

                                                                                                          aCCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGACTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTg                                                                                                                                >  2:282557/1‑239 (MQ=255)

                                                                                                              gCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  1:166693/204‑1 (MQ=255)

                                                                                                                cgggttaatctgtACCAAAGCGGCCCAGATAACCAATCTCACATTATTTAGGGGAACCATGAGAGGC‑GGTCCCACAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCGCTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCggg                                                                                                                          <  2:979602/227‑1 (MQ=255)

                                                                                                                   aGTTTTCGTTACCAAAGCGGCCCAGAGAACCAATCCCCCATGATTTAGGTGAACCAGGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGaa                                                                                                                        <  2:726597/238‑1 (MQ=255)

                                                                                                                             aCCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTggcg                                                                                                              >  2:683082/1‑238 (MQ=255)

                                                                                                                             aCCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAAATTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGGCTCACTGGCATTTTTCTCTTCATTTTTGTCCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAAACGATTggcgg                                                                                                             >  2:1213410/1‑239 (MQ=255)

                                                                                                                              ccAAAGCGGCCCAAAGAACCAATCGCCAATGATTTAGGTGAACCATGAGAGGC‑GGACCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTATCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTggcggc                                                                                                            <  2:1250032/239‑1 (MQ=255)

                                                                                                                               cacagcgagccAAAGAACCAAATCCTCTTGATTGAGGTGAACAAGGAGAGGC‑GGTCCCCCAA‑CCAGACC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTAATGGAATAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCCCTACTGGTCGGGAACGGATTGGCGGCa                                                                                                           <  1:210422/230‑1 (MQ=255)

                                                                                                                               cAAAGCGGCCCAAGAAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCa                                                                                                           <  2:1325577/239‑1 (MQ=255)

                                                                                                                                aaaGCGGCCCAGAGAACCAAGCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCAGTTTTCTCTTCATGTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCAt                                                                                                          <  2:1186558/239‑1 (MQ=255)

                                                                                                                                      gCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCCGAAC‑GGTAATAGCCGTTGTAAATAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCCTTTTTGACCAGTACAGCATCAGGTTTTGCGGCCCTCATGGGTGGGAACGGATTGGGGGGAAACACtt                                                                                                    >  1:1053955/1‑239 (MQ=255)

                                                                                                                                              acccattgggccagtgtttAGGTGTACCACAAGCGGC‑GGTACCACAC‑CCAGAAA‑GGTAAGAGCCGTTGTACAGAAA‑GCCAATTTCATTGTTCCCAAATTTC‑CTGAAATCTTTCGGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCATTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAGCGGATGTGCGGCATACCCTTGATCAtt                                                                                             <  1:717051/222‑1 (MQ=255)

                                                                                                                                               aCCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCg                                                                                           <  2:265099/239‑1 (MQ=255)

                                                                                                                                                 cAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCCGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCCATGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTTGGGCACTCCTGGTCGGGAAAGGGTTGGGGGCAAAACCCTTATCATTCgaa                                                                                         >  1:679296/1‑237 (MQ=255)

                                                                                                                                                         cATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCt                                                                                 <  2:429203/239‑1 (MQ=255)

                                                                                                                                                          ctgttttAGGTTACCCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTa                                                                                <  1:683082/235‑1 (MQ=255)

                                                                                                                                                                     gAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCg                                                                      >  2:770565/1‑238 (MQ=255)

                                                                                                                                                                       aCCATGAGTGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCATTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCg                                                                   <  2:253975/239‑1 (MQ=255)

                                                                                                                                                                       aCCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCg                                                                   <  2:942829/239‑1 (MQ=255)

                                                                                                                                                                       aCCAGGAGGGGC‑GGTCCCCCAA‑CCATAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCg                                                                   <  2:784913/239‑1 (MQ=255)

                                                                                                                                                                        ccATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAACCTGTCTCACTTGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGTTCGGGAACGGAATTGCGGCCAACCCCTTTTCCTTTGCACTTTCCCTACGAttacccgcccc                                                                  >  1:1162721/1‑230 (MQ=255)

                                                                                                                                                                              gagGC‑GGTCCCCCAA‑ACAGAAC‑GGTAATAGCCTTTTTAACTAAA‑CCCAATTTCATCTTTCCCAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCg                                                             <  1:431399/238‑1 (MQ=255)

                                                                                                                                                                                  c‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGtat                                                          <  2:268150/237‑1 (MQ=255)

                                                                                                                                                                                  c‑GGTCCCCCAA‑CCAAAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTACCAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGtat                                                          <  2:1219241/237‑1 (MQ=255)

                                                                                                                                                                                    ggTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCCGAGTCATGGAAGAATAAATCCAGCCTGTCCCACTGGCCTTTTTCTCTTCATTTTTTACACATACACCATCAGGGTTTTCGGCCCTCCTTGTCGGGAACGGGTTGGCGGCATAACCTTTGTCCTTCGTACTTACCCCACGACTCaaccgtgcgggcggtaaat                                                       >  1:1284154/1‑221 (MQ=255)

                                                                                                                                                                                            aa‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGCCGTATACTTTttct                                                 >  2:273772/1‑237 (MQ=255)

                                                                                                                                                                                                    aC‑GGTAATAGGCGTTGTAACTAAA‑GCCAATTTCCTCTTTCACAAATTTA‑CTGAAATCTTTCAGGGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTtcttctctt                                           <  2:194490/236‑1 (MQ=255)

                                                                                                                                                                                                    aC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTtcttctctt                                           <  2:849909/236‑1 (MQ=255)

                                                                                                                                                                                                          aaTTGCCTTTTTAAATAAA‑GCCAATTTCATATTTCACAAATTTA‑CTGAAATCTTTCAGAGTCAAGGAAGAATAAATCGGGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTta                                   <  1:770565/239‑1 (MQ=255)

                                                                                                                                                                                                                  ttGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCtt                                                                                                                                                                         >  2:963559/1‑97 (MQ=255)

                                                                                                                                                                                                                  ttGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACGGCCGCTGGGGTTTACTTTTTCTTTCTTTCATTTTTCAAAttttt                           >  1:932339/1‑239 (MQ=255)

                                                                                                                                                                                                                  ttGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCa                                         <  2:1198049/225‑1 (MQ=255)

                                                                                                                                                                                                                   tGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCCTTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTg                                                                                                   >  1:688897/1‑166 (MQ=255)

                                                                                                                                                                                                                   tGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGAc                                                                                                                                                               <  2:1206095/106‑1 (MQ=255)

                                                                                                                                                                                                                   tGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTg                                                                                                   <  2:688897/166‑1 (MQ=255)

                                                                                                                                                                                                                   tGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTg                          >  2:1088061/1‑239 (MQ=255)

                                                                                                                                                                                                                   tGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGCCGCTGGCGTATACTTTTTCTTTTTTTATTCTTTAAACttttta                          >  1:293451/1‑238 (MQ=255)

                                                                                                                                                                                                                   tGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGACATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTgcc                                                                                                                                                               >  1:1206095/1‑104 (MQ=255)

                                                                                                                                                                                                                       aCTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGCTTGGCGGCATACCCTTGATCATTCGTACTTACGCTCCGATTCACCGTCGCTGGCGTATACTTTTTCTTTCTTTCATTTTTATCCttttttggt                       >  1:531525/1‑234 (MQ=255)

                                                                                                                                                                                                                          aaa‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGAt                                                                                                 <  1:30505/161‑1 (MQ=255)

                                                                                                                                                                                                                          aaa‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGAt                                                                                                 >  2:30505/1‑161 (MQ=255)

                                                                                                                                                                                                                            a‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGGTAATTCGTACTTCGGCTACGATTCACCGTCGCTGGCGTATCCTTTTTCTTTCCTTCATCTTTATAtttctgctcctaataa                 >  1:1198049/1‑226 (MQ=255)

                                                                                                                                                                                                                                  aTTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGt            >  2:1052590/1‑239 (MQ=255)

                                                                                                                                                                                                                                  aTTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGt            <  2:458439/239‑1 (MQ=255)

                                                                                                                                                                                                                                   tttCTTCTTTCCCATATTTT‑GTGAAATCTTTAAGCGTCATGGAAGAATAAATCGAGCCGGTCTCCCTGGCATTTTTCTGTTCATTTTTGACCCGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGtt           <  1:1213410/239‑1 (MQ=255)

                                                                                                                                                                                                                                    tttatttttCACAAATTTC‑CTGAAATTTTTCAGCGTCATGGAAGAAAAAATGGGGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATGCCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGttt          <  1:1095884/233‑1 (MQ=255)

                                                                                                                                                                                                                                         ttttCACAAATTTT‑CTTAATTCTTTCAGAGTTATGGAAGAATAAATCCAGCCTGGCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATTCCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCtt     <  1:951534/238‑1 (MQ=255)

                                                                                                                                                                                                                                          tttCACAAATTTA‑CTGAAAACTTTCAAAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCGCTTCATTTTTGAACAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATGCTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTa    <  2:416754/239‑1 (MQ=255)

                                                                                                                                                                                                                                             cacaAATTTA‑CTGAAATCTTTCAGAGTCCTGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTAGTCCTTACGCTACGATTCACCGTCGCTGGCGTCTATTTTTTCTTCTCTTCATCTTTCTACTTTTTGAGTTCGCTTTGCTTTTCTCTTaaa  >  1:199929/1‑237 (MQ=255)

ACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACAC‑GTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGC‑GGTCCCCCAA‑CCAGAAC‑GGTAATAGCCGTTGTAACTAAA‑GCCAATTTCATCTTTCACAAATTTA‑CTGAAATCTTTCAGAGTCATGGAAGAATAAATCGAGCCTGTCTCACTGGCATTTTTCTCTTCATTTTTGACCAGTACAGCATCAGGTTTTGCGGCACTACTGGTCGGGAACGGATTGGCGGCATACCCTTGATCATTCGTACTTACGCTACGATTCACCGTCGCTGGCGTATACTTTTTCTTCTCTTCATCTTTATACTTTTTGAGTTCGCTTTGCGTTTCTCTTAAC  >  NC_000913/3901631‑3902100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`