BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,932,711	A→G	11.2%	E466G (GAG→GGG)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,711	0	A	G	11.2%	264.4 / 3.6	113	E466G (GAG→GGG)	kup	potassium transporter
Reads supporting (aligned to +/- strand): ref base A (41/56); new base G (12/2); total (55/58)
Fisher's exact test for biased strand distribution p-value = 3.19e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 115 total aligned reads displayed.

GTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAA  >  NC_000913/3932474‑3932946

gTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATC‑CTGATTGCTGTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGgag                                                                                                                                                                                                                                              <  2:1187942/239‑1 (MQ=255)

 tCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGgaga                                                                                                                                                                                                                                             >  2:965400/1‑239 (MQ=255)

  caaaaCTGGCACTGGAATAAGTTTTTTGTTGCGCTGATC‑CTGATTGCTTTCCTTTGG‑GTCGATATTCCATTGTTCACCGCTACCCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGaa                                                                                                                                                                                                                                            <  2:575259/236‑1 (MQ=255)

  cAGAACTGGCATTGGAATAAGT‑TTTTTTTGGGCTGTTCTCTGATTGGTTTCCTTGGG‑GTGGATATTCCATTGTCCACCGCTAACCGCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGaa                                                                                                                                                                                                                                            <  1:192421/239‑1 (MQ=255)

  cAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGGGaa                                                                                                                                                                                                                                            >  2:1149123/1‑239 (MQ=255)

                  aaaaGTATTTTGTTGCGCTGATC‑CTGTTTGCTTCCCTTGGT‑GTGGATATCCCATTGTTCCCCGCTAACCTCGATAAACT‑GCTCCCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTcgc                                                                                                                                                                                                                            <  2:447451/237‑1 (MQ=255)

                  aTAAGTTTTTTGTTGCGCTGATC‑CTGATTGCTTCCCTTGTT‑GTCGATTTTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGACCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTcgc                                                                                                                                                                                                                            <  2:829876/239‑1 (MQ=255)

                     aGTATTTTGTTGCGCTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGGACATGGTAACCCCCTG‑‑GAAAGGATGATTGCTTCCCTGGAaaaaaaacccccatgtcccggg                                                                                                                                                                                                                         >  1:65296/1‑221 (MQ=255)

                            ttttgCGTTGTTC‑TTGATTGTTTTCCTTTGT‑GCGGATTTTCCATTGTCCACCCCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGTTGGCCATTGAGCCTTGGTTCTGTGATGTTTAG‑CGTG‑ATGGCCCCCTGGAAGAGCGAGCGTTTCCGCTGGCTGGGGCGGATGCATGAACATGGTAACTCTCGG‑‑GAAGCGATGATTGCTTCGTTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGa                                                                                                                                                                                                                 <  1:1123177/237‑1 (MQ=255)

                             ttaagaaccgtccttgtttGTTTTCTTTTGT‑GTCGATGTGCCTTTGGTCCCCCCCAACCCCTATAACGC‑GCTCCCCGGGGGCTGGTTGCCTTGGGCCCCCGGTACGGTGTTGTTTCT‑CGTG‑ATGACCACCGGGAGGAGCGAGGTTTTCCGTTGGCTGCGGGGGATGCATGAACATGGTCACCCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGa                                                                                                                                                                                                                 <  1:373228/222‑1 (MQ=255)

                               tgCGTTTATC‑CTGATTGCTTCCCTTTGT‑TTCGATATTCCCTTGTTCCCCGCTAACCTAGATAAACT‑GCTCTCGGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGAcc                                                                                                                                                                                                               <  2:1163782/238‑1 (MQ=255)

                               tgCGCTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcg                                                                                                                                                                                                              <  2:1088383/239‑1 (MQ=255)

                                tttttGATC‑TTGTTTG‑TTTCCTGTGTGGTCGATTTTCCATTGTTCCCCTCTAACCT‑GATAAAATGGCCCTCCGGCGGCTGGTTGACATTGAGCCTCGTTTCTGGGATTTTTAG‑TGTG‑ATGAACACCTGGAAGAGCGAGCTTTTCCGCTTGCTGCGGCGGATGAATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcgc                                                                                                                                                                                                             <  1:433197/235‑1 (MQ=255)

                                tgtcgttttttttgttGTTTTCCTTTGT‑TGCTATTTTCCTTTGTTCCACGGTAACCTGGAAAAGCT‑TCTCCCCGGCGGCTGGTTGCCATGGAGCCTGGGTACTGTGATGTTTGT‑CGTG‑ATGCCCACCTGGAAGGGCGAGCGTTTCCGCTTGCTGGGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGTGATGATTGCTTCGTTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcg                                                                                                                                                                                                              <  1:594183/225‑1 (MQ=255)

                                tgccgtgacccgggttGCTTTCTTTTGT‑GGCGTTGTTCCATTGTGCCCCCTCAACCCGGATAACCTCGCTC‑CCGGGGGCGGGTTGCCATTGGGCCCGGGTCCTGGGA‑GTTTGA‑CGGGTATGCCCCCCGGGAAGGGCGAGCGTTTCCGCTTGCTGCGGCGGATGCAGGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcg                                                                                                                                                                                                              <  1:1066287/225‑1 (MQ=255)

                                gctcTGTTC‑TTGTTTGTTTTCTTTTGT‑GTCGATATTCCATGTTTCCCCGCTAACCCCGATAAACT‑GTCCCGCGGCGGCTGGTTGCCATTGACCCTCGTTACTGTGATGTTTAC‑CGTG‑ATGACCACCTGGAAGAGCGAGGGTTTCCGTTTGCTGGGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcgc                                                                                                                                                                                                             <  1:307782/236‑1 (MQ=255)

                                gcgcTGATC‑CTGATTGCTTTCCTTTGG‑GTCGTTATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcgc                                                                                                                                                                                                             <  2:1210666/239‑1 (MQ=255)

                                 tacTGTTC‑TTGTTTGCTTTTCTTTGT‑GTGTTTATTCCTTTGTCCACCGCAACCCTGGATAACCT‑GCCCTCCGGCGCCTGGTGGCCATGGCGCCTCGGTACTGTGTTGTTCAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcgc                                                                                                                                                                                                             <  1:13481/236‑1 (MQ=255)

                                 gtgtggttcgtgtttGTTTTCTTTTGT‑GTGGTGTTTCCATTTTTCCCCTCTAACCGAGATAAACG‑TCTCCCCGGGGGTGGGTTGCCATGGAGCCTGGGTTCTGTGATTTTTAT‑GGTG‑ATGCCCCCCTGGAAGAGCGAGCGTTTCCGCTTGCGGCGGCGGATGCATGAACATGGTACCTCTCGG‑‑GAGGCGATGATTGTTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcgc                                                                                                                                                                                                             <  1:223949/226‑1 (MQ=255)

                                 gtgcttttcttttttGGTTTCCTTTGG‑GCAGATTTTCCATTGTTCGCCGCTCATCTTAATAATCT‑GCTCTCCGGGGGATGTTTGCATTTGAGCCTGGGTACTGTGATGTTTAT‑GGTG‑ATGACCACATGGAAGAGCGGGCGTTTCCGCTTTCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTGCGCTGGAGAAATCTCCGCCTGTTCGCGTGC‑CCGGGACcgc                                                                                                                                                                                                             <  1:963307/226‑1 (MQ=255)

                                 cgcTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATTAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGGGAAATAACCGCCTGTTCGCGGGC‑CCGGGACcgcg                                                                                                                                                                                                            >  2:495425/1‑239 (MQ=255)

                                 cgcTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGGGAAATCACCGCCTGTTCCCGGGC‑CCGGGACcgcg                                                                                                                                                                                                            >  2:753608/1‑239 (MQ=255)

                                   cTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTGGATAAACG‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCgg                                                                                                                                                                                                           <  2:511424/238‑1 (MQ=255)

                                             aTTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑TAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCCCGGGGTATA‑TTTc                                                                                                                                                                                                >  2:122627/1‑239 (MQ=255)

                                             aTTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAAATCTCTG‑‑GAAGCGATGATTGCTTCGCTGGGGAAATACCCGCCTGTTCGCGTGC‑CCGGGACCCCGGTGTATT‑TGt                                                                                                                                                                                                 >  2:938104/1‑238 (MQ=255)

                                                 cTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTGACTCTCTG‑‑GAAGCGATGATTGCTTTGCTGGAGAAATCACCGCCTGTTCGCGTGA‑CCGGGACCGCGGGGTATA‑TGTCGCGt                                                                                                                                                                                            >  2:340818/1‑239 (MQ=255)

                                                 cTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTCTA‑TGTcgccg                                                                                                                                                                                            >  2:942789/1‑237 (MQ=255)

                                                 cTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGGGTATA‑Tg                                                                                                                                                                                                  >  2:13481/1‑233 (MQ=255)

                                                 cTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTC‑‑GAAGCGATGTCTTTTTCGCGGGAGAAAAAACCCCCTTTTCGCGGGG‑CCCGGAGCACGGGGtgtagatggggg                                                                                                                                                                                             >  1:351237/1‑231 (MQ=255)

                                                 cTTTCCTTTGC‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAGAAGCGATGATTGCTTCCCTGGGGAAATCACCGCCCGTTCCCTGGC‑CCGGGGCCCCGGGGTTTA‑TGTggc                                                                                                                                                                                              >  2:1143958/1‑239 (MQ=255)

                                                   ttCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAGGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGACAATGGTAAATCTCTG‑‑GAAGCGAGGATTGCTTCGCTGGGGAAATCACCGCCTGTTCGCGGTC‑CCGGGACCGCGgtggttctggctctttt                                                                                                                                                                                           >  2:373228/1‑225 (MQ=255)

                                                   ttCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGGGTATA‑TGTCCCGTGc                                                                                                                                                                                          >  2:624085/1‑239 (MQ=255)

                                                           t‑gtCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGGAGCATGACCAATGTGAACCTCTG‑‑GAAGGCATGGTTGCTTTGCTGGTGAAAACACCGCCGTTTCGCGTGCACCGGGACCCGGGGGTata‑ttgcctgggaataaaaa                                                                                                                                                                                   >  1:191445/1‑223 (MQ=255)

                                                           t‑gtCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGGGCAATCAAc                                                                                                                                                                                   >  2:17250/1‑238 (MQ=255)

                                                           t‑gtCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCCCggctgttccttttacacatctccga                                                                                                                                                                                    >  2:1065734/1‑215 (MQ=255)

                                                             gtCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGGGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCCCGGGGTATATTTTCGCG‑GCAATCaaag                                                                                                                                                                                  >  2:491785/1‑236 (MQ=255)

                                                             gtCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAAAATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGGGAAATCACCGCCTGTTTGCGTGC‑CCGGGACCCCGGGGTATA‑TGTCGCGTGGAATCACCg                                                                                                                                                                                  >  2:238199/1‑238 (MQ=255)

                                                              tCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGCACATGGTAACCCTCTG‑‑GAAACCATGATTGCTTCGCTGGAGAAAACACCGCCTCTTCTCGGGC‑CCGGGACCCCGGGGTATA‑TGTGTCGTGCAATCAACgac                                                                                                                                                                                >  1:1003292/1‑237 (MQ=255)

                                                                gATTTTCCATTTTTCACCGCTAACCTCGATAAACT‑GCTCCCCGGCGGTTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCCCCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCAt                                                                                                                                                                              <  2:918199/239‑1 (MQ=255)

                                                                 atatTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCCCGGGGGATT‑TGTCCCGTGCAATCAACGGCAt                                                                                                                                                                              >  2:432138/1‑238 (MQ=255)

                                                                 atatTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGACTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGGGCAATCAACGTCAt                                                                                                                                                                              >  2:82439/1‑238 (MQ=255)

                                                                     tCCATTGTTCACCGCTACCCTGGATAAACG‑GCTCTCGGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCt                                                                                                                                                                         <  2:883417/239‑1 (MQ=255)

                                                                         ttGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGCATATGGTAACTCTCTG‑‑GAAGCGCTAGTTGCTTCGCTGGGGAAAACACCCCCTGCTCCCGTGC‑CCCGGCCCGCGGTGGCTA‑TGTCGCGTGAAAACAAAGTCATTCCCCTTgc                                                                                                                                                                     >  1:296821/1‑239 (MQ=255)

                                                                            ttCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGGGTATA‑TTTCGCGGGCAATCAACGTCATTCCCTTTgg                                                                                                                                                                     >  2:52261/1‑235 (MQ=255)

                                                                            ttCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGGGGATA‑TGTGCCGTGCAATCAACGCCCTTCCCtttcg                                                                                                                                                                     >  2:761174/1‑234 (MQ=255)

                                                                              cACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGGAACCTGCTG‑‑GAAGCGATGATTGCTTCGCTGGGGAAATCACCCCCTGTTTGCGCTC‑CCGGGGCCCCGGGGTGTA‑TGGCCCGGGGAATCAACCCCATTCCCCTTgccgtgg                                                                                                                                                                >  1:1031760/1‑234 (MQ=255)

                                                                              cACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCCAGCGTTTCCGCTTGCTGCGCCGGCTGTATGAACACGGGAACTCTCTG‑‑CAAGCGACGATTGGTCTCCTGGAGAGATCCACGCCTCTTCTGGAGC‑CCGGGACCGCGGTCTTTA‑TGTCGCGGGGAATACACCTCGTTCCCtttccccgc                                                                                                                                                                 >  1:710131/1‑232 (MQ=255)

                                                                                  gttAACCCCGATAAACT‑GCCCTCCGGCGGCGGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCCCCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCGG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCa                                                                                                                                                            <  2:774687/237‑1 (MQ=255)

                                                                                  gatAACCTCGAAAAGCG‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTTGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCa                                                                                                                                                            <  2:407141/237‑1 (MQ=255)

                                                                                  gCTAACCCCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCa                                                                                                                                                            <  2:351237/239‑1 (MQ=255)

                                                                                      aCCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGGGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGGGGATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATaa                                                                                                                                                         >  2:366064/1‑238 (MQ=255)

                                                                                             tAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGCCCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTaaa                                                                                                                                                  <  2:1003292/238‑1 (MQ=255)

                                                                                             tAAACT‑CCTCTCCGGGGGCTGGTTGCCATTGAGCCTCGGTTCTGGGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGGGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTaaa                                                                                                                                                  <  2:236792/238‑1 (MQ=255)

                                                                                             ataaattgtctctcgggggggtgTTGCCATGGGGCCTGGGT‑CTGTGGTTTTTTTACGGG‑ATGCCCACCGGGAAGAGGGGGCGTTGCCGGTTGGTGCGGAGGAGGAATGAACATGTTAACTCCCGG‑‑GAAGCGATGATTGCTTCGTGGGGGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTaaa                                                                                                                                                  <  1:151210/217‑1 (MQ=255)

                                                                                                     cctccggggggcTGGTTGCATTTGAGCCTCGGTATTGTAATTTTTAT‑CGTG‑ATGACCCCCTGGAAGAGCGAGCGTTCCCGATTGCTGGGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACaa                                                                                                                                          <  2:1208809/230‑1 (MQ=255)

                                                                                                        tcCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGGAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGGAATAACCGCCTGTTCGCGTGG‑CCCGGACCGCGGGGTATA‑TGTCGCGGGCAAACAACGTCATTCCCTTTGCGCGGATGGATAACCTTaaaaaaaaaaaaga                                                                                                                                       >  1:1152742/1‑228 (MQ=255)

                                                                                                                tgggTTGCCTTGTACCCTGGGTTCTGTGATGTTTAC‑GGTG‑AGGACCACGTGGAAGCGCGAGGGTTTCTGCTTGCTGCGGCGAATGCATAAACATGGTAACTCCCTG‑‑GAAGCAATGATTGCTTCGCTGGACAACTCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCAt                                                                                                                                <  2:928931/236‑1 (MQ=255)

                                                                                                                  ttttGCCTTTGGGCCGCGGTCTTGTTGTGTTTTC‑TGTG‑ATGCCCACTGGGAATGTCGGGGGTTCCCGTTGGCTGCGGCGAATGTATAACAATGGTAACTCTTGG‑‑GAGGGGATGATTGCTTCGTTGGAGAAACCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAg                                                                                                                             <  1:366064/237‑1 (MQ=255)

                                                                                                                  ggTTGCCATGGGGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCCCCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGa                                                                                                                              <  2:1201346/238‑1 (MQ=255)

                                                                                                                      tccATTGAGCCCTGGTACTGTGATTTTTAC‑CGTG‑ATGACCCCCTGGAAGGGGGGGCGTTTCCGCTTGCTGGGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCggg                                                                                                                         <  2:896820/238‑1 (MQ=255)

                                                                                                                        cATAGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCCCCTGGAAGAGCGAGCTTTTCCGCTTGCGGCGGCGGATGCAGGAACATGGTAACTCTCTG‑‑GAAGGGAGGTTTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACcgc                                                                                                                                                                                                             <  2:1280092/154‑1 (MQ=255)

                                                                                                                         atggAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCCCGTGGAAGAGCGAGCGTTTCCGCTTGCTGGGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTg                                                                                                                       <  2:1045812/235‑1 (MQ=255)

                                                                                                                          ttGGGCCTCGGTCCTGTGATGTTTAT‑CTTG‑ATGCCCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTACCTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGAt                                                                                                                     <  2:1152742/239‑1 (MQ=255)

                                                                                                                          ttGAGCTCCGGTACTGGGATGTTTAT‑CGTG‑ATGACCCCCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGAt                                                                                                                     <  2:191445/239‑1 (MQ=255)

                                                                                                                             aGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGGAAATCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCCGTTCGCGTGC‑CCGGGACCGCGGGGGATA‑TTTCTCGGGCCATCCACGTCATTCCCTTTTCGCCGATGGATAAACTTAAACAAAAAAAGGTATTGCCTGAGGGGGGGGTTCt                                                                                                                  >  1:896820/1‑239 (MQ=255)

                                                                                                                               ccTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAAACTTAAACACAACAAGGGATTGCATGAGCGGGTGATTCTGt                                                                                                                >  2:1233397/1‑239 (MQ=255)

                                                                                                                                cTCGGTACTGTGATGTTTAC‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATAAACTTGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGt                                                                                                                <  2:743932/238‑1 (MQ=255)

                                                                                                                                     ttcTGTGTTTTTTAT‑CGGG‑AGGACCACCTGGAAGAGCGAGGGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCGG‑‑GAAGCGATGATTGCGTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAAct                                                                                                           <  1:122627/236‑1 (MQ=255)

                                                                                                                                       cTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGCAAATCTCTG‑‑GAAGCGATGATTGCTTCCCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTCTA‑TGTCGCGTGCAAGCAACGGCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACCAGGAATTGCTTGAGCGGGGGATTCGGTTAActat                                                                                                         >  2:1257387/1‑236 (MQ=255)

                                                                                                                                               tttAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCCCGGGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGGATTGCATGAGCGGGGGATTCTGTTAACTCTTCGCACCGa                                                                                                >  2:533932/1‑239 (MQ=255)

                                                                                                                                                 tAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGGGCAATCAACGTCATTCCCTTTGCGCTGATGCTTAACCTTAAACATAACAAGGAATTGACTGAGCGGGGGATTCTTTTAACTCTGCGCACCGaaa                                                                                              >  2:1194913/1‑238 (MQ=255)

                                                                                                                                                 tAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCGGTTCTCGTGC‑CCCGGGCCACGGGGTGTA‑TTTTCCGTCAAATCACCGTCATTCCCCTTGCGCCGGTGCATAACCTTTAACATAACAAAGAATTGCATGAGCCGGGGAtgtcgttaaactctagccaccaaa                                                                                              >  1:195219/1‑216 (MQ=255)

                                                                                                                                                 tAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCCCTT‑‑GAAGCGGTGATTGCTTCGCTGGGGGAATCAACGCCTGTTTGCGTGC‑CCGGGGCCCCGGTGTTTA‑GTTCGCGTGCAATCAACGTCATTTCCTTTGGGCCGGTGCGTAACCTTAAACAAAAAAAAGGATTGCAAGAGCGGGTGATTCTGGTaaaaccacgcccccaaa                                                                                              >  1:107176/1‑224 (MQ=255)

                                                                                                                                                     cGTG‑ATGACCACCTGGAAGAGCGAGTTTTTCCGCTTGCTGCGGCGGATGCATGAACTTGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACg                                                                                           <  2:747893/239‑1 (MQ=255)

                                                                                                                                                     cGTG‑AAGCCCCCCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACg                                                                                           <  2:65296/239‑1 (MQ=255)

                                                                                                                                                          atgaCCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGGGGTTCTGTTAACTCTGCGCACCGAAGACGCTcc                                                                                       >  2:1004654/1‑239 (MQ=255)

                                                                                                                                                          atgaCCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGCATTGCATGAGCGGGTGATTCTGTTAAATCTGCGCACCGAACACGcccc                                                                                       >  2:1256048/1‑239 (MQ=255)

                                                                                                                                                               caccTGGAAGGGCGAGGGTTTCCGGTTGCTGCGGCGGATGCATGAACATGGTAACTCTCGG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATg                                                                                  <  2:762605/239‑1 (MQ=255)

                                                                                                                                                                           cgagcgTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAAAAAAACAAGGTATTGCATGAGCGGGGGTTTTTGTTAACTCTGCGAACCGTAGAAGCTCCATATGTCCAGAActtcc                                                                      >  2:63364/1‑239 (MQ=255)

                                                                                                                                                                              gttttttCGTTTGTTGGGGGGGATGCATGAACTGGGTAGCTCTCTG‑‑GAAGCGATGATTGTGTCGATGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgtccgt                                                                    <  1:533932/235‑1 (MQ=255)

                                                                                                                                                                              gtttttCCCGTTGCGGCGGGGGATGCAGGAACAGGGTAACTCTCGG‑‑GAAGCGATGGTTGCTTCGTGGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgtccgt                                                                    <  2:646390/235‑1 (MQ=255)

                                                                                                                                                                                        tgggcgggggggaggaaTGAACAGGGAAACCCTTGG‑‑GAGGCGGGGATGGCTTCGCTGGAAAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGa                                                          <  2:1285006/222‑1 (MQ=255)

                                                                                                                                                                                                        aagaaatgggTAACCCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCGTGTTCGCGTGC‑CCGGGACCGCGGTTGATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGccc                                         <  2:281860/231‑1 (MQ=255)

                                                                                                                                                                                                           aaCATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACt                                      >  2:403107/1‑239 (MQ=255)

                                                                                                                                                                                                                ggTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGCGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTCCAGATTGAACAACTGTCGCCCACTTTCTg                                 >  2:761217/1‑239 (MQ=255)

                                                                                                                                                                                                                ggTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGc                                           >  2:9249/1‑229 (MQ=255)

                                                                                                                                                                                                                      tctcgG‑‑GAAGCGATGATTGCTCCGTTGGAGAAATCACCGCCTGTGCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCAc                                                                                                   <  1:621142/167‑1 (MQ=255)

                                                                                                                                                                                                                      cttctG‑‑GAAGCGATGATTGCTTCGCTGGAAAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAc                                                                          <  2:822074/190‑1 (MQ=255)

                                                                                                                                                                                                                        tctG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTACACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGCCCATAACGTCCGTCGGGTACCGATTGAACAACTGTGGCCCACTTTCTGGCCCgtgg                         >  2:1146250/1‑239 (MQ=255)

                                                                                                                                                                                                                          tG‑‑GAAGCGATGATTGATTCGCTGGAGTAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGGGCAATCAACGTCATGCCCTTTGCGCTGATGCCTAACCTTCAACATAACAAGGTATTGCATGAACGGGGGATTCTGTTAACTCTGCGCACCGACGACACTCCATGTGACCATAACGTCCGTCGGGTCCAGATTGAGCAACTGTCGCCCACATTCTGGCGCgtgggg                       >  1:810459/1‑237 (MQ=255)

                                                                                                                                                                                                                             tggggCGATTGTTGGTTCCGTGGAGAAATCCCCCCCTGTTCGCGTGC‑CTGGGACCGCGGTGTATA‑TGTCGCTTGCAATCAACGTTATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGtggtgg                      <  1:974178/235‑1 (MQ=255)

                                                                                                                                                                                                                              gAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTAAAGATTGAAAAACGGTCGCCCACTTTCGAGCGCGgggggga                     >  1:167308/1‑238 (MQ=255)

                                                                                                                                                                                                                                  cGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCt                                                                                         >  2:621142/1‑167 (MQ=255)

                                                                                                                                                                                                                                  cGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGGGGTGGCAGGt                 >  2:1276563/1‑239 (MQ=255)

                                                                                                                                                                                                                                    atgatTGCTTCGTTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCa                                                                                                                                                                                     <  1:401339/73‑1 (MQ=255)

                                                                                                                                                                                                                                    atgatTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCa                                                                                                                                                                                     >  2:401339/1‑73 (MQ=255)

                                                                                                                                                                                                                                    atgatTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCTTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCCGGTTa               >  2:835059/1‑239 (MQ=255)

                                                                                                                                                                                                                                      gatTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATg             >  2:885719/1‑239 (MQ=255)

                                                                                                                                                                                                                                           cTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGCCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTgg        >  2:165060/1‑239 (MQ=255)

                                                                                                                                                                                                                                              cGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCACAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGa                                                                                             <  2:218587/151‑1 (MQ=255)

GTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATC‑CTGATTGCTTTCCTTTGT‑GTCGATATTCCATTGTTCACCGCTAACCTCGATAAACT‑GCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTAT‑CGTG‑ATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTG‑‑GAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGC‑CCGGGACCGCGGTGTATA‑TGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAA  >  NC_000913/3932474‑3932946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`