BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,943,487	T→G	13.1%	noncoding (54/76 nt)	gltU →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,943,487	0	T	G	13.1%	164.5 / 3.2	118	noncoding (54/76 nt)	gltU	tRNA‑Glu
Reads supporting (aligned to +/- strand): ref base T (19/75); new base G (21/2); total (41/77)
Fisher's exact test for biased strand distribution p-value = 2.78e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACAC  >  NC_000913/3943249‑3943724

cTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑A‑CGGGGt                                                                                                                                                                                                                                                        >  2:829768/1‑239 (MQ=14)

    aCCTTCGGGAGGGCGCTTACCACTTTGTGAATCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGa                                                                                                                                                                                                                                                    <  2:324962/239‑1 (MQ=14)

     ctttCGGAAGGGCGCTTACCCTTTTGGTATTTAGGGCGGGGGTGAATTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTGGGATCACCTTCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAAATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAGGCAGACTGATCCGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGaa                                                                                                                                                                                                                                                   <  1:2535/237‑1 (MQ=11)

     ccTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGaa                                                                                                                                                                                                                                                   <  2:201672/239‑1 (MQ=31)

     ccTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGaa                                                                                                                                                                                                                                                   <  2:855395/239‑1 (MQ=31)

     ccTTCGGGAAGGCTCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGaa                                                                                                                                                                                                                                                   <  2:752645/239‑1 (MQ=14)

     ccTGCGGGAGGGCTCTTCCCCTTTTGTGATTCATGACTGGGGGGAAGTCGTAACAAGGTAACCGTAGGGGACCCTGCGGTTGGATCCCCTCCTTTCCTTAAAGAAGCGTTCTTTTCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGaa                                                                                                                                                                                                                                                   <  1:233701/239‑1 (MQ=11)

     acTTCGGGAGGGCCCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGaa                                                                                                                                                                                                                                                   <  2:903337/238‑1 (MQ=14)

        tCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGCCTAGAGGCCCAGGAAACCGCCCTTTCACG‑GCGGTATA‑A‑AGGGGttgaaac                                                                                                                                                                                                                                                  >  1:1141572/1‑233 (MQ=9)

          gggAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑ACGGGGTCGAAT‑ccc                                                                                                                                                                                                                                              >  2:108633/1‑238 (MQ=14)

             ggggCGTTTACCCCTTTGTGATTCGTGACGGGGGTGAAGTCGTAACAAGGTAACCGTACGGGAACGTGCGGTGGGATCACCTCCTTACCATAAAGAAGCGTTCTGTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTa                                                                                                                                                                                                                                           <  1:913243/237‑1 (MQ=11)

               ggCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGGTCGAAT‑TCCCTCggg                                                                                                                                                                                                                                        >  2:466221/1‑239 (MQ=14)

               ggCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AAGGGTTCCAAT‑cccccaccg                                                                                                                                                                                                                                        >  2:15667/1‑234 (MQ=14)

               ggCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAAAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTGTGGAA‑CCCCTAagg                                                                                                                                                                                                                                        >  2:654170/1‑239 (MQ=9)

                gccgttACCCCTTTGTGATTCATGGCGGGGGTGAAGGCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAggg                                                                                                                                                                                                                                        <  1:829768/234‑1 (MQ=9)

                   cTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGCCACCGCCCTTTCACG‑CCGGAA‑C‑A‑AAGGGTTTGCAT‑ccccactgggag                                                                                                                                                                                                                                     >  2:256757/1‑230 (MQ=11)

                     tcccACTTTGTGATTCATGACTGGGGGGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGcc                                                                                                                                                                                                                                  <  2:971257/237‑1 (MQ=14)

                           tttGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAATCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAATGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGGA‑A‑C‑AAGGGGTTGCAA‑CCCCCAGGGGACGccccctg                                                                                                                                                                                                                             >  2:141765/1‑233 (MQ=11)

                           tttGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGCTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCACTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTC‑A‑C‑AGGGGGTCGAAT‑TCCCTAGGGGGCGccccct                                                                                                                                                                                                                              >  2:1162877/1‑233 (MQ=255)

                           tttGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAAAGGCCCAGGCAACCGCCCTTTCACG‑GCGGCA‑A‑A‑CGGGGGATGAATACCCCCAGGGGTAGCCACtga                                                                                                                                                                                                                             >  1:737584/1‑237 (MQ=11)

                           tttGTGATTAAGGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGc                                                                                                                                                                                                                            <  2:522190/239‑1 (MQ=14)

                             tgtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CGCACACAGATTGTCTGATAGGAAGTGAAAGGCAAGGCGTCTTGCGAAGCAGACTACTCCGTCCCCTTCGGCTAAAGACCCAGGACACCCCCCTTTCACG‑GCGCGA‑A‑C‑AGGGAGTCGAAT‑CCCCTCAGCGACGCCAcatcgtc                                                                                                                                                                                                                          >  1:35439/1‑233 (MQ=11)

                             tgtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGGG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACTGCCCCTTCGCCTAGAGGCCCAGGCCACCGCCCTTTACCGCGGGGAA‑A‑A‑AGGGGTCCAACA‑CCCCCGGGGGACGCCCCtggtg                                                                                                                                                                                                                           >  1:1150812/1‑235 (MQ=11)

                                      tggttgtggtaaattggagagaaGGTAACAGGATGGTAACCATCGGTTGGATCACCGTCTTACCTGTAAGTAGAGTTCGGTGCGGTG‑CTCACACAGATTGTCTGATGGGAAGGGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCTCCTGCGTATAGAGTCCCAGGACACCGCCCTTTCACG‑GGGGTA‑A‑C‑AGGGGTTCAAAT‑CCCGTAGGGGACGCCACTTGCT‑GGTTTgttag                                                                                                                                                                                                                <  1:1186275/218‑1 (MQ=2)

                                       gACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACAGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGaa                                                                                                                                                                                                                                                   <  2:70736/205‑1 (MQ=14)

                                            gggTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGGTCGAAT‑CACCCAGGGGACGCCCCTTGCT‑GGGTTTTGGGTGAAAg                                                                                                                                                                                                          >  2:153602/1‑239 (MQ=255)

                                                taatttttAACAAGGTAACCGTGGGGGAACCGGCGGTTGGATCACCGCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑TTCCAACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGTGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑c                                                                                                                                                                                                     <  1:870488/232‑1 (MQ=11)

                                                gatctCGTAACAAGCTAACCGGTTGGAATATTGTGGTTTGATCGCCTCCTTACCTTAAAGAAGCGTTCTTTGGAGTG‑CTCACACAGAGTGTCCGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCACCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑c                                                                                                                                                                                                     <  1:455658/235‑1 (MQ=11)

                                                  aGTCGTAACAAGGTAACCGTTGGGGAACCTGCGGTTGGATCACCTCCTTACCATAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑cc                                                                                                                                                                                                    <  2:737584/238‑1 (MQ=14)

                                                  aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑cc                                                                                                                                                                                                    <  2:558505/238‑1 (MQ=31)

                                                  aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑cc                                                                                                                                                                                                    <  2:378335/238‑1 (MQ=31)

                                                      gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑ACCCCAGGGGGGGCCCCCTTCT‑GGGTTTTGAGGGAAAGACA‑CATGCCt                                                                                                                                                                                               >  2:679147/1‑239 (MQ=11)

                                                        aaCAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCCCTTGCT‑GGGTtttggggggaaggtccccgccttt                                                                                                                                                                                              >  2:449263/1‑219 (MQ=11)

                                                              gTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑ACGGGTTTGCAT‑CCCCCAGGGGGCCCCCCCTTCT‑CGTTTGTTGGTGGAAAGCA‑CCCGCCCT‑Cataatg                                                                                                                                                                                      >  1:1055538/1‑236 (MQ=11)

                                                              gTAACCGTAGGGGAACCTGCGGTTGGATCAACTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGCCACCGCCCTTTCACG‑GCGGTT‑A‑C‑ACTGGTTCTAAT‑ATCCCAGGGGACGCCACTTTCT‑CTGTTTTGATGGGAAGACC‑CCTGCCtttaaaat                                                                                                                                                                                        >  1:1113271/1‑232 (MQ=11)

                                                                 accgggggggAAGCTGGGGTGGGATCACCCGCTTGCCTTAAAAGAGCGTTCTGTGCAGGG‑CTCCAAAGGATTGGCGGATAGGAAGTGAAAAACAAGGCGGCGTGCCAAGGAGACTGATACGTCCGCGTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCaa                                                                                                                                                                                   <  1:285381/233‑1 (MQ=11)

                                                                   cGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGc                                                                                                                                                                                                                            <  2:579435/199‑1 (MQ=31)

                                                                       ggggAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑A‑AGGGGGTCGAAA‑TCCCCACGGGGCGGCACTTGGTGGGTTTGTGGGTAAAATTC‑‑CCCGCCTT‑TATAACTCAAAACC‑Ca                                                                                                                                                                            >  2:540489/1‑239 (MQ=11)

                                                                             ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGCA‑A‑CAAGGGGTTGAACT‑CCCCCAGGGG‑CGCCACTTTCT‑GGTTTGTGGGTGAGAGGAC‑CCTCCCCT‑AAAATCTCaaaaacaatactcg                                                                                                                                                                       >  1:764955/1‑229 (MQ=11)

                                                                             ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTCGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AAGGGGTCCAAG‑CCCCCCGGGGGCCCCACTTTCT‑GGGTTTTTGGTGGAACTCA‑CACCCCTT‑AAAATATCAAAACA‑CATCtctg                                                                                                                                                                      >  1:1164997/1‑236 (MQ=11)

                                                                                   gTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTACAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑ACGGGGGCGAAT‑CCCCCAAGGGACGCCCCTCGCC‑GGGTTTTTAGTGGAAGTAC‑ACTGCCTT‑ATTATCCCCAAACT‑CACCTTTGGGGGag                                                                                                                                                                >  2:667832/1‑238 (MQ=11)

                                                                                      ggATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAAGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGGA‑A‑CAAGGGGTTC‑AAT‑CCCCTAGGGGGCGCCACCTGCT‑GGGTTTGGAGTGAAAGTCA‑ACTGCCTT‑AATATTTCAAAACT‑AATATTCGGGTGATG‑tt                                                                                                                                                            >  2:1027679/1‑239 (MQ=11)

                                                                                       gttcctctcctTTCCTTAAAGAAGGGTTTTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCGTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑ttt                                                                                                                                                           <  1:449263/233‑1 (MQ=9)

                                                                                         tcccctcctTACCTTAAAGAAGCGTTCGTTGCAGTT‑CTCACAAAGGTTGGCTGATAGGAAGTGAAACGCAAGGCTTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTg‑a                                                                                                                                                        <  2:180514/236‑1 (MQ=11)

                                                                                                  taccttaAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCC‑GGGTTGTTAGTGAAAGTCA‑CCTGCCTT‑AAAATCTCTAAAAT‑CATCTTCGGGGGGTG‑ttttgggaaattgt                                                                                                                                                >  2:898072/1‑228 (MQ=11)

                                                                                                   tccttaAAGAGTGGTTCTTTGCATTG‑CTCAACAAGATTGACTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGTGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGct                                                                                                                                             <  1:466221/238‑1 (MQ=11)

                                                                                                   tccttaAAGAAGCGTTCTTTGCAGGG‑CTCACCCAGATGTTCGGGTAGGAAGTGAAAGGGAATGCGTCGTGCTAAGCAGTCTGATACGTCCCCTTCGTCTAGAGGCCCAGGACCCCGGCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGct                                                                                                                                             <  1:487407/238‑1 (MQ=11)

                                                                                                   cccttaAAGAGTGGTCATTTGCAGGT‑CTCACGCAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTTTTGCCAAACAAACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGTTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTAAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGct                                                                                                                                             <  1:180031/238‑1 (MQ=11)

                                                                                                   accttaAAGTAGCGTTTTTTGCCGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGct                                                                                                                                             <  1:108633/239‑1 (MQ=14)

                                                                                                        aaaGAAGCGTTCTTTGCAGTG‑CTCACACAGGTTGGCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATGCGTCCCCTTCGTCTAGAGGCCCAGGACCCCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTa                                                                                                                                        <  2:394678/239‑1 (MQ=9)

                                                                                                        aaaGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTa                                                                                                                                        <  2:958052/239‑1 (MQ=31)

                                                                                                         aaagagCGTTCTTTGCAGTGCCTCAC‑CGGATTGCCGGAAAGGAAGTGAAAACCAAGGCGTCTTGCGAAGCAGACTGTTACGTCCCCTTCGTCTAGAGGCCCAGGACCCCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTaa                                                                                                                                       <  2:511620/235‑1 (MQ=11)

                                                                                                          agaagCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCCAGGGGAGGCCACTTTCT‑GGGTTGTGAGGGAAAGTCA‑CCTGCCTT‑CATATCTCAAAACT‑CATCTTCGGGGGGTG‑TTTG‑GGGTA‑ttttgccttttaa                                                                                                                                      >  2:832164/1‑229 (MQ=11)

                                                                                                              gCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGCA‑A‑C‑CAGGGGTCGAAT‑CCCCCAGGGGACGCCCCCTGCT‑GGGTTTTTAGGGAAAGGCA‑CCCGCCTTCAAATTCCCAAAAC‑‑CGACTTCGGGGGAGG‑TTTT‑AGAAATTTTGCTTTTaaaaaaa                                                                                                                                   >  1:923255/1‑238 (MQ=11)

                                                                                                               cGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGGTCGAAT‑CCCCCCGGGGCCGCCCCCTGCC‑TGGTTGTGTGGGAAACGCACCCTCCCTA‑CATTACTC‑AAACT‑CCTCTTCGGGTGGTG‑TTTT‑TAGCA‑TTTTCTTTTTTAAAATCt                                                                                                                                 >  1:149380/1‑239 (MQ=2)

                                                                                                               cGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑ACGGGGTCGAGT‑ACCCCAGGGGACGGCACTTGCT‑GGGTTGTGAGTGGAAAGCA‑CCTGCCTT‑AATATCTCAAAACA‑CATCCTCGGGGGGTG‑GTTT‑AGAAC‑TTTTTTCTTTtaaaaaca                                                                                                                                 >  1:1178733/1‑236 (MQ=11)

                                                                                                               cGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AAGGGGTCGCAT‑CCCCCAGGGGACCCCACCTGCT‑GGTTTGTGAGTGAAAGGCA‑ACTGCCTT‑AATATCTCAAAAAT‑CCTCTTCCGGTGATG‑TTTT‑AGATA‑TTTTCTCTTTaaaaa                                                                                                                                    >  2:1278673/1‑236 (MQ=11)

                                                                                                                           tggcccctacaGATTTTTTGACTGGACGTCAAAAGCAGGGCGTGTGTCGAATCCGACTGATACGTCCCGCTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTg                                                                                                                      <  1:1162877/231‑1 (MQ=11)

                                                                                                                           tG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGc                                                                                                                                                                                                                            <  2:109597/143‑1 (MQ=32)

                                                                                                                           tG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAAt                                                                                                                                   <  2:1061542/225‑1 (MQ=31)

                                                                                                                           tG‑CTCACAAAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTg                                                                                                                      <  2:140686/238‑1 (MQ=14)

                                                                                                                            g‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCCCTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAAAT‑CATCTTCGGGGGAGG‑GTTGTAGA‑A‑TTTGCTCTTTAAAAATCTGGGTCAAGCTGaa                                                                                                                    >  2:1187155/1‑239 (MQ=11)

                                                                                                                              cTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGGCTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGaa                                                                                                                    <  2:86452/238‑1 (MQ=14)

                                                                                                                              cTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑ACC‑AGGGACTC‑AAT‑CCCCCAGGGGGACCCACCTGCC‑GGTTGTTGAGTGGAAGAGA‑CCTGCCTT‑CAAATTTCAAAAAT‑CAATCTCGGGGGATGATATA‑GGGAA‑TTTTCT‑TTTAAAAAACTGGATCAAAATGaaa                                                                                                                   >  1:985141/1‑239 (MQ=2)

                                                                                                                              cTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGGTCGAAT‑CCCCCCAGGGACGCCACCTGCT‑GGTTTGTGAGGGGAAGGCA‑ACCGCCTT‑AATATCTCAAAAATCCTTTTTCGGTGGGTG‑TTTG‑‑GATC‑ATTGTTCTTTAAAAAtattgacaaaaaaataa                                                                                                                   >  1:1273216/1‑223 (MQ=11)

                                                                                                                                  cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTg                                                                                                               <  2:35439/239‑1 (MQ=31)

                                                                                                                                   acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCggg                                                                                                                                                                    <  2:59148/188‑1 (MQ=31)

                                                                                                                                      gATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑A‑AGGGGGTCGAAT‑CCCCTCAGGGGCGCCACCTGCT‑GGGTTGTGAGTGAAAGGCA‑CCTGCCTT‑AATATCTCAAAACA‑CCTCTTCGCGGGATG‑TTTT‑AGGTA‑TTTTCTCTTCTAAAAACTGTGTCAAGccagtctctttaaa                                                                                                           >  1:1061542/1‑226 (MQ=11)

                                                                                                                                       gaaaagggacaGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATTCGGCCCCTTCGTCCAGAGGCCCAGGGCCCCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCAGGTGATG‑TTTG‑AGATA‑TTTGCGCTTTAAAAATCTGGATCAAGCTGAAAATTGAAaca                                                                                                          <  2:1154636/229‑1 (MQ=11)

                                                                                                                                        ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGAGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAaca                                                                                                          <  2:457627/238‑1 (MQ=14)

                                                                                                                                        ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGGTCGAAT‑CCCCCAGGGGACGCCACTTGCT‑GGGTTTTGAGGGAAAGGCA‑CCTGCCTT‑AAAATCTCAAAACT‑CACTTTTGGGTGATT‑TTTT‑AGGAA‑TTTTCCCTTTTAAAAACTTGGTCAAGCCGGAAATTTaaaa                                                                                                           >  2:1029592/1‑236 (MQ=11)

                                                                                                                                           tCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑CAGGGGTCGAAT‑CCCCTAGGGGACGCCCCTTGCT‑TGGTTGTGGGTGACAGTCA‑ACCGCCTT‑AATATCTCAAAAAT‑CACCTTCGGGGGAGT‑GTTG‑TGATA‑TTTTCTCTTTTAAAAACTGGGTAAACCTGAAAATTTAAAAACtca                                                                                                      >  2:1134191/1‑237 (MQ=11)

                                                                                                                                           gcTGATAGGAAGTGAAAAGCAAGGCGTTTTGCGAAGCAGACTGATACGTCCCCTTCGTTTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGa                                                                                                      <  2:1205039/238‑1 (MQ=14)

                                                                                                                                            cggATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGa                                                                                                      <  2:864184/236‑1 (MQ=14)

                                                                                                                                             tGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATTCGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGaac                                                                                                    <  2:19758/239‑1 (MQ=14)

                                                                                                                                             tGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTTTGAGTGAAAGTCA‑CCTGCCTT‑AATATTTCAAAACT‑CATCTTCGGGGGATG‑TTTG‑AGATA‑TTTTCTCTTTTAAAATCTGGGTCAACCTGAAAATTTAAACACTGaac                                                                                                    >  2:95187/1‑239 (MQ=11)

                                                                                                                                               aTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑c                                                                                                                                                                                                     <  2:298709/145‑1 (MQ=32)

                                                                                                                                                 aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGGTCGAAT‑CCCCTAGGGGACGCCCCTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATAACTCAAAACT‑CATCTTCGGGTGATG‑GTTG‑CGATA‑TTTGCTCCTTTAAAATCTTGATCAAGCTGAAAATTTAAACACTGAACAACg                                                                                                >  2:884900/1‑239 (MQ=11)

                                                                                                                                                                                                      tAGGGGCTCAGGACACCGCCCTTTCACG‑GGTTTA‑A‑C‑AGGGGTTCGAAT‑CCCCGAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTTCCTT‑AATATCACAAATCT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaaga                                          <  2:1142176/239‑1 (MQ=11)

                                                                                                                                                                                                        gagGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGGCC‑CCTGCCTT‑AATATCCCAAAACT‑CATCTTCGGGTGATG‑TTTT‑GGATA‑TTTTCTCTTTAAAAATTTTGATCAAACTGCAAATTTAAAAACTGAACAACGAAAG‑TTTTTTGTTAACTTCCCAAATTTTTGCAACCCCATGATGAATCgaaagaaa                                        >  2:1171283/1‑239 (MQ=11)

                                                                                                                                                                                                              cAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGGTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGGGGGAAAGTCA‑CCTGCCTT‑AATATTTCAAAACT‑CACCTTCTGGGGATG‑TTTG‑AGATA‑TTTTCTCTTTAAAAAACTGGATCAAGCCGAAAATTGAAACACCAAAAAACGAAAA‑TTGTTTGTGGGTGTCTCAAATTTTTGCACAACGATAATGAAACGAAAAAAAAAACtt                                  >  1:1013419/1‑239 (MQ=11)

                                                                                                                                                                                                                ggACCCCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg                                <  2:973007/239‑1 (MQ=14)

                                                                                                                                                                                                                      ccccttttCCCG‑GGGGTT‑A‑A‑AGGGGGTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGGTAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTtgt                          <  1:153602/234‑1 (MQ=11)

                                                                                                                                                                                                                      cccccTTTCACG‑GCGGTA‑A‑C‑TGGTGTTCCAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTTGGAGTGAAAGTCA‑ACTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCACTTTAAAAAACTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTtgt                          <  1:540489/237‑1 (MQ=11)

                                                                                                                                                                                                                      cGCCCTTTCACG‑GCGGCA‑A‑C‑AGGGGGTCGTAT‑TCCCCAGGGGGCGCCACTTCCT‑GGTTTTCGACTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACA‑CATCTTCGGGTGGTG‑TTTG‑AGATA‑TTTTGTCCTTAAAAATACAGAGCACTCTGTAAAGTTGAACAATGAACAACGAACGATTGTTTGT‑AGTCTCTAAAATTTTCGCAACACGAAAGTGAAAAGAAAAAAAAATATTCGGGAtgt                          >  1:410382/1‑239 (MQ=2)

                                                                                                                                                                                                                                     tttA‑A‑C‑AGGGTTTGGAAT‑CCCCTAGGGGACGCCATTTGTG‑GGTTTGGGAGTGAAAGTCA‑CCTGGCTT‑AATATCACAAAGCT‑CAACTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  1:679147/236‑1 (MQ=11)

                                                                                                                                                                                                                                     gttA‑A‑C‑AGGGGTTCGCAT‑CCCCTAGGGGTCGCCATTGTCT‑TGTTTGGGAGGGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGGTATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  1:1187155/236‑1 (MQ=11)

                                                                                                                                                                                                                                     ggTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCC‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  2:242275/238‑1 (MQ=14)

                                                                                                                                                                                                                                     ggTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  2:1150812/238‑1 (MQ=31)

                                                                                                                                                                                                                                     ggTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  2:880856/238‑1 (MQ=31)

                                                                                                                                                                                                                                     ggTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  2:1151169/238‑1 (MQ=31)

                                                                                                                                                                                                                                     ggTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGGGAAAGTCA‑CCTGGCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  2:144269/238‑1 (MQ=14)

                                                                                                                                                                                                                                     ggTA‑A‑A‑GGGGGTTTGAAT‑CCCCGTGGGTACCCCACTTGCT‑GGTTTGTGAGTGGAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACGCTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt            <  1:1075991/239‑1 (MQ=11)

                                                                                                                                                                                                                                       tA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTa           <  2:879976/238‑1 (MQ=31)

                                                                                                                                                                                                                                        gggagccggggTTCGAAG‑CCCGTAGGGGAGGCCACTTGGT‑GTTTTGTGAGTGAAAGTTA‑CCTGCCTT‑AAAATCTCAAATCT‑CATCTTCGGGGGATG‑TTTG‑AGATA‑TTTGCTGATTAAAAATCGTGATCAAGCTGAAAATTGAACCACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc             <  1:1278673/231‑1 (MQ=11)

                                                                                                                                                                                                                                        a‑a‑C‑AGGGGTTTGAAT‑CCCCTAGGGGACGCCACTTGCT‑TGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTaa          <  1:507379/238‑1 (MQ=14)

                                                                                                                                                                                                                                        a‑a‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTaa          <  2:217744/238‑1 (MQ=31)

                                                                                                                                                                                                                                                 ggTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAAAACGATGATGGATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca   >  2:639453/1‑239 (MQ=14)

                                                                                                                                                                                                                                                  gTTCGAAT‑CCCCTAGGGGGCGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATC‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTTAACAACGAAAG‑GTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGAGGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca   <  2:1185762/238‑1 (MQ=9)

                                                                                                                                                                                                                                                  gTTCGAAT‑CCCCGAGGGGACGCCAATTGCT‑GGTTTGTGAGTTAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTACGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca   <  2:671324/238‑1 (MQ=9)

CTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTG‑CTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACG‑GCGGTA‑A‑C‑AGGGGTTCGAAT‑CCCCTAGGGGACGCCACTTGCT‑GGTTTGTGAGTGAAAGTCA‑CCTGCCTT‑AATATCTCAAAACT‑CATCTTCGGGTGATG‑TTTG‑AGATA‑TTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAG‑TTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACAC  >  NC_000913/3943249‑3943724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`