Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,956,960 T→C 8.9% E287G (GAG→GGG)  ilvY ← transcriptional activator of ilvC, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,956,9600TC 8.9% 345.2 / 2.3 134E287G (GAG→GGG) ilvYtranscriptional activator of ilvC, autorepressor
Reads supporting (aligned to +/- strand):  ref base T (47/71);  new base C (7/7);  total (54/80)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCA  >  NC_000913/3956722‑3957198
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
tatctcgaccATGGACCCGACTACGGGGTCGTACTGGCGGCTGTCGGACTTGGCGCCAATGAACCGGATTGCGAAACCCGGCTGAATTAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGGTAGGGAAAAATGCCTGATAGCGCTGCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCGTTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCt                                                                                                                                                                                                                                                   <  1:795489/231‑1 (MQ=255)
  tCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGACTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCCTTTTGGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCa                                                                                                                                                                                                                                                 >  2:362262/1‑239 (MQ=255)
    gCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGGAAAATGCCTGATAGCGCTTCGC‑TTATCAAGCCTACCCGGGC‑GCCAACGTACTTTTTGGTTTGGCCAAA‑TCTTCCAGAA‑TGGCTCAAt                                                                                                                                                                                                                                               >  2:243006/1‑239 (MQ=255)
       gCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGGAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGCCCTTTGTGGTTCGGCCAAA‑ACTTCCCGCA‑TTCCCCAATTa                                                                                                                                                                                                                                             >  2:12367/1‑238 (MQ=255)
         cATGGCACCGACTACGGGCGCGTACGGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCg                                                                                                                                                                                                                                          <  2:343029/239‑1 (MQ=255)
           tGGCACCGACTACGGGAGCGTACCGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGAGTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGc                                                                                                                                                                                                                                        <  2:842781/239‑1 (MQ=255)
            ggCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCCGGTTCTTTTTGGC‑GGGGTAGGGGAAAAAGCCTGGTAGCGCTTCCC‑TTTTTAGGCCCACCCGCGC‑GGCAAAGTCCTTTTTGGTTCGGCAAAA‑ACTTCCACAA‑AGCCTCaaatcagggg                                                                                                                                                                                                                                        >  1:908362/1‑230 (MQ=255)
            ggCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGGTAGCCCTTCGC‑TTATCAGGCCTACCCCCGC‑GACAACGTCATTTGTGGTTTGGCAAAA‑ACTTCCATAA‑TTCCTCCATTaatggg                                                                                                                                                                                                                                        >  2:305933/1‑233 (MQ=255)
               aCCGACTTCGGGGGGGTCCGGGCGGCGTTCGATCTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTTGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTGTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCa                                                                                                                                                                                                                                     <  1:428656/238‑1 (MQ=255)
                  ggcttcgggcgggTATTGGGGGCGTTCGAACTGGGCGACCCTGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTa                                                                                                                                                                                                                                 <  1:950106/234‑1 (MQ=255)
                  gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GCCAACGGCCTTTGTGGGTCGGCAAAA‑TCTTCCCGAA‑TGCCCCAATTAGCGGccccttta                                                                                                                                                                                                                                 >  2:262257/1‑232 (MQ=255)
                   aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCCCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCCCCATTAACGGCCCCTGTa                                                                                                                                                                                                                                 >  2:242675/1‑238 (MQ=255)
                    ctgcGGGCGCCTACTGGCGGCTTTCGAACCTGGGCACCAGGAACCGGATTTCGAAACCCGGCTGAATGTGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTGGGC‑TGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑c                                                                                                                                                                                                                              <  1:799459/236‑1 (MQ=255)
                             cgTACTGGTGGCGTTCGAACTTGGCGACCTTGAACCGGATTTCGAAACCCGGCTGAATGCGCTGGGCTACGATTGCCACGACGAAACCCATAAACCGGCGTTCAGGTTCTTTTTGAC‑GGGTTAGGGGAAAAATCCCGCTAGCGCCTCGCTTTCTTTGCCTTCCCCGCGC‑GCCAAGACCTTTTGTGTTTCGGC‑AAA‑TCTTCCAAAA‑TGCCCCCACTACCGGCCCCTGTCT‑CCGCcttttt                                                                                                                                                                                                                     >  1:272532/1‑238 (MQ=255)
                             cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGGTAAGGGAAAAAGCCCTGAAAG‑GCCTCCC‑TTTTTATGGCTCACCCCGC‑GAGAACGGCCTTTGTGGGTTGGCCAAA‑TTTTCACAAA‑TGCCCCCATTAACGGCTccctgaacccccttttt                                                                                                                                                                                                                      >  1:243108/1‑223 (MQ=255)
                             cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCCTTTAG‑CCGCtttttt                                                                                                                                                                                                                     >  2:447233/1‑238 (MQ=255)
                             cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCCCGC‑GCCAACGGCATTTGTGGTTCGGCAAAA‑TCTTCCCGAA‑TGCCTCAATTAGCGGCCCATGTAA‑CCGCtttttt                                                                                                                                                                                                                     >  2:21033/1‑238 (MQ=255)
                               tACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGGGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCt                                                                                                                                                                                                                    <  2:704352/238‑1 (MQ=255)
                                 cTGGCGGCGTTCGAACTTGGCGCCCATGAACCGGATTGCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTgcg                                                                                                                                                                                                                 <  2:441540/239‑1 (MQ=255)
                                      ggcgTTCGAACTTGGCAACCATTAACCGGATTACGAAATCCGGCTGAAGGAGCTGGGCTACGATTGCCACGACGAAGCCAATAACCCGGCCTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGcaca                                                                                                                                                                                                             <  2:189799/238‑1 (MQ=255)
                                           gtgAACTTGGCGACCCTTAACCGGATTTCGAAACCCGGCTGAATGGGCTGGGCGACGGTTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGc                                                                                                                                                                                                        <  1:15218/236‑1 (MQ=255)
                                            ctaaCTTGGCGCCCATGACCCGGAATTCGAAACCGGGGTGAATGGGCTGGGCTACTATTGCCCGGCCGAAACCAATCACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGAGAGCGCTTCGC‑TTATGAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGccc                                                                                                                                                                                                      <  1:1010262/237‑1 (MQ=255)
                                            cGAACTTGGCGACCTTGAACCGGATTTCGAAACCCGGCGTAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACATCGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTATTTCTGCGCACACACGccc                                                                                                                                                                                                      <  2:1067829/239‑1 (MQ=255)
                                               gcTTGGCGACCATGAACCGGATTTCGAAACCCGGGTTAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCGTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAg                                                                                                                                                                                                    <  2:861098/237‑1 (MQ=255)
                                                          cggAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTAAGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGt                                                                                                                                                                                        <  2:1228403/237‑1 (MQ=255)
                                                               ctggATTCCGCACCCCGGCTGAATGGGCTGGGCTGCGTTTTCCACGAGGAAACCAATATCCCGGCGTTCAGGTTCTTTTTGCG‑GGGCTAGGGAAAAATGCCTGATAGCGCCTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTtct                                                                                                                                                                                   <  1:151599/237‑1 (MQ=255)
                                                                   aTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACCCCCCGCTCAAACGGCGGTTTCTCAt                                                                                                                                                                                >  2:336835/1‑238 (MQ=255)
                                                                   aTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAAGGAAAAATGCCTGATAGCGCTTTCC‑TTATCAGGCCTCCCCGCGC‑GACAACGTCCCTTGTGGTTCGGCAAAA‑TATTCCAGAA‑CGCCCCAATTACCGGACCATGTAGCCCGCTTTTTTGGGCCACA‑ACGCCCCACCCAAACCGGGTTTTTTCaac                                                                                                                                                                               >  1:736705/1‑237 (MQ=255)
                                                                       cGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTg                                                                                                                                                                           <  2:870921/239‑1 (MQ=255)
                                                                        gAAACCCGGCTGGATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTg‑c                                                                                                                                                                         <  2:1281019/239‑1 (MQ=255)
                                                                         aacccccGCTGAATGGGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTg‑cg                                                                                                                                                                        <  1:813911/236‑1 (MQ=255)
                                                                         aaccccGGCTGTATGGGTTGGGCTTCGTTTGCCACGGAGAAACCAGTAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTg‑cg                                                                                                                                                                        <  1:362262/236‑1 (MQ=255)
                                                                         aaccccGGCTGAAGTGGCGGGGTGACGGGTGCCACGACGAAACCAATAACCGGGGGTTTGGGTTCTTTTTGGC‑GGGGTAGGGAAAAAGGCGTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTg‑c                                                                                                                                                                         <  1:170961/235‑1 (MQ=255)
                                                                                     aTGAGCTTGTGTACGATTGACTCGAAGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAatca                                                                                                                                                             <  2:773908/238‑1 (MQ=255)
                                                                                       gAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTACCGGCTCATGTAG‑CCGCTTTTTCTTCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAatcat‑c                                                                                                                                                          >  2:577270/1‑238 (MQ=255)
                                                                                         gCTGGGCTTCGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAAtcat‑ca                                                                                                                                                         <  2:717315/237‑1 (MQ=255)
                                                                                         gCTGGGCTACGATTGCCACGACGAAACCAATACCCCGGCGTTCAGGTTCCTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAAtcat‑ca                                                                                                                                                         <  2:675085/237‑1 (MQ=255)
                                                                                                  cGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGGTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCACTTAGCGGCTCATTAAG‑CCGCTTTTTTTCTCCCCACACGCCCCGCTCAAACGGCGTTTTCTCACCCCTGCCCCTCT‑AAATCAC‑CAACCGGTTaac                                                                                                                                               >  1:748560/1‑236 (MQ=255)
                                                                                                    ttgtgccccaggaACCCCATACCCCGGTCTTTCGGTTGTTTTTGGT‑GGGTTAGGGAAAAATCCCTGTTTGCGCTTTCC‑TTTTCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTTG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCAc                                                                                                                                            <  1:243006/228‑1 (MQ=255)
                                                                                                     ttGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCAc                                                                                                                                            <  2:318140/238‑1 (MQ=255)
                                                                                                     ttGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCAc                                                                                                                                            <  2:983541/238‑1 (MQ=255)
                                                                                                     ttGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCAc                                                                                                                                            <  1:398885/238‑1 (MQ=255)
                                                                                                       gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTCACGGCCCATGTAG‑CCGCTTTTTTTGCGCACACACGCCCAGCTCAAACGCCGTTTTCTCTACGCTG‑CGCTCTAAATTCAT‑TACGCGGGTCCGCAcccg                                                                                                                                         >  2:96900/1‑237 (MQ=255)
                                                                                                       gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCGTTTTGGC‑GGGTTAGGGAAAAATGCCTGGTAGCGCTTCGC‑TGATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCAc                                                                                                                                            <  2:135949/236‑1 (MQ=255)
                                                                                                           cgacgaAACCAATAACCCGGCGGTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCg                                                                                                                                     <  2:643673/239‑1 (MQ=255)
                                                                                                               gaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGc                                                                                                                                 <  2:908362/239‑1 (MQ=255)
                                                                                                                       tAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTcc                                                                                                                         <  2:1269865/239‑1 (MQ=255)
                                                                                                                           ccgggttctcggTTTTGTTTGGC‑GGGTGAGGGAAAAATGCGTAGAGGTGCTCCTC‑TTAGACGGCCAGGCGGCTC‑GACAACGTCATTTGTGTTCCTGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGGAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGca                                                                                                                     <  1:1223290/229‑1 (MQ=255)
                                                                                                                            cttccgttcgtttttttttGGC‑GGGTTTGGGAAAAATGTCTGATAGCGTTTCGC‑TTACTGAGCCTACCCGCTC‑GACCACGTCATTTTGGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGcac                                                                                                                    <  1:541929/225‑1 (MQ=255)
                                                                                                                              gCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCCAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGcccc                                                                                                                   >  2:71057/1‑238 (MQ=255)
                                                                                                                              gCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGGTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGGTAAGCACCGGTTCGGGGCTGGTTTCCAGcacc                                                                                                                   >  2:841798/1‑238 (MQ=255)
                                                                                                                               cGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAAAGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACAGCGTTTTCTCATCGCTG‑CGGTCTAAACTAAT‑CACGCGGGTACGCCCCGGTTCGGGGCTGTTTTCCAGcacc                                                                                                                   >  2:404367/1‑237 (MQ=255)
                                                                                                                               cGTTCAGGTTCTGTTTGGC‑GGGGTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTAACAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGcacca                                                                                                                  <  2:514189/238‑1 (MQ=255)
                                                                                                                                   cAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCCTTTAA‑CCGCTTTTTCTTCGCCAACACCCCCAGCCCCAAAGGCGGTTTTTCATTCCTG‑CGCCTCAAAACAATCCCCGCGGACACCCA‑CGGTTCGGGGGCGTTTTCCaaccacacaccc                                                                                                             >  1:546730/1‑228 (MQ=255)
                                                                                                                                   cAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTcc                                                                                                             <  2:170507/239‑1 (MQ=255)
                                                                                                                                         cTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACCCACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTGCGGGGCGGTTTTCCCGAACACCTTCCGGcaac                                                                                                       >  2:817307/1‑239 (MQ=255)
                                                                                                                                         cTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGcaac                                                                                                       >  2:1225147/1‑239 (MQ=255)
                                                                                                                                                  ggggTTAGGGAAAAATGCCTGATAGCCCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgcca                                                                                                <  2:245152/237‑1 (MQ=255)
                                                                                                                                                    ggTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCg                                                                                                                                                                                                                                          >  2:745946/1‑101 (MQ=255)
                                                                                                                                                    ggTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCg                                                                                                                                                                                                                                          <  1:745946/101‑1 (MQ=255)
                                                                                                                                                     gtcgcgcgggtaggCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TTCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCCTCGCTG‑CGCTCTAAAATCAT‑CACCCGGTTACGCACCGGTTCGGGGCTGGTTTCCAGCACCCCTTCCGGCAACAATgccacgc                                                                                             >  2:94012/14‑238 (MQ=255)
                                                                                                                                                         tggAAAAGTCCCTGATCGCTCTTCCT‑TTAGCGGCCCTACCCGCGC‑GGCAACGTCTTTTGTGGTTCGGCAAAA‑CCTTCCAGAA‑GGCCTCAATTAGCGGCTCCTGTGG‑CCGCTTTTTCTCCGCACACCCGCCCAGCTCAAACGGCGTTTTCTCAGCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCCCCACTTCCGGCAACAATGCCACGCCACAg                                                                                        <  1:262257/238‑1 (MQ=255)
                                                                                                                                                            aaaaaTGCCTGATTGCGCTTCGC‑TTATCAGGCCTACCCGGCG‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGcc                                                                                      <  2:301528/238‑1 (MQ=255)
                                                                                                                                                            aaaaaTGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GAAAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGcc                                                                                      <  2:414605/238‑1 (MQ=255)
                                                                                                                                                             aaatggacTGATTGCCCTTTGC‑TTTTCAGGCCTACCC‑CGCGGAAAACGGTTTTTTTGGTTCGGCAAAA‑CCTTCCAGAA‑TCCCTCAATTAGCGGCTCATGTAG‑CCGTTTTTTCTGCGCACACACGCCCAGCCCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCg                                                                                     <  1:270671/231‑1 (MQ=255)
                                                                                                                                                             aaaaTGCCTGGTAGTGCTTCGA‑TTAACAGGCCTACCCGGGC‑GACAACGTCATTTGTTGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTGG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCg                                                                                     <  2:690359/238‑1 (MQ=255)
                                                                                                                                                                    cTGATAGCGCTTCGC‑TTAGCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGcca                                                                              <  2:253037/238‑1 (MQ=255)
                                                                                                                                                                        tAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATc                                                                         >  2:120805/1‑239 (MQ=255)
                                                                                                                                                                           ctcttcgc‑ttATACGGCCTTCCCCGGG‑GACACCGGTCTTTGTGGTGTGGGAAAA‑TCTTCCAGAA‑GGCCCCAATTAGCGGCTCAGGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCAGCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGa                                                                       <  1:384982/236‑1 (MQ=255)
                                                                                                                                                                             cttcgc‑ttATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTCAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGGTGTTTTCCAGCCCCCCTTCCGGCAAAAAAGCCCCCCCCCAGCCCGGTGCCACCAACGaca                                                                     >  1:876270/1‑236 (MQ=255)
                                                                                                                                                                                cgc‑ttATCAGGCCTACCCGCGG‑GAAAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATgccacgcca                                                                                           <  2:1135685/213‑1 (MQ=255)
                                                                                                                                                                                        cggcccTCCCCGCGC‑CACAACGTCTTTTGTGGTGCGGCAAAA‑TCTTCCAGCA‑TGCCCCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCa                                                          <  1:120805/235‑1 (MQ=255)
                                                                                                                                                                                           gCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGGGCCACCATCGATACCATCGCTTCATg                                                        >  2:438225/1‑238 (MQ=255)
                                                                                                                                                                                              tACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCCCCATCGATACCATCGCTTAATGccc                                                     >  2:814022/1‑238 (MQ=255)
                                                                                                                                                                                                cccGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCCCCGGTTCGGGGCTGTTTTCCAGAACCACTTCCGGCAACAATGCCACGCCACAGCCCGGGGCCACCACCCATACCAACGCTACATGcccccc                                                  >  1:525830/1‑239 (MQ=255)
                                                                                                                                                                                                    cctcgaacatgttttttGTGTTGCCGCAAAA‑TCTCCCCGAA‑GGCCCAAATTAGCGCCTCATTTTG‑CCCCTTTTTCTGCCCACACCCGCCCAGCTCAACCGGCGTTTTCTCAGCGCTG‑CGCTCAAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCACCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAAc                                               <  1:814022/225‑1 (MQ=255)
                                                                                                                                                                                                     gc‑gACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATTTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTcc                                                                                                             <  1:1073266/175‑1 (MQ=255)
                                                                                                                                                                                                     gc‑gACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGGGCCACCACCGTTACCATCGCTTCATGCCCGCCAACCg                                             >  2:731109/1‑239 (MQ=255)
                                                                                                                                                                                                      ccggaactcttgttttttgttgtgCAAAA‑GTTTCCAGAA‑TGCCCTATTTGGCGGCTCAGTTAG‑CCGCTTTTTCTGCGCACACCCGCCCTGCTCAGACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAC‑CACGCTGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCg                                             <  1:577270/216‑1 (MQ=255)
                                                                                                                                                                                                      c‑gACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGt                                            >  2:675463/1‑239 (MQ=255)
                                                                                                                                                                                                            gcttttttttttttctggaaaat‑TCTCCCAAAA‑TGCCCCAACTAGGCGCCCATGTAG‑CCGCTGTTTCGGCCCACACCCGCCCCGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGt                                       <  1:21033/220‑1 (MQ=255)
                                                                                                                                                                                                                  ttttgggTTTGGGAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTGATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCCCCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatca                                 <  1:336835/234‑1 (MQ=255)
                                                                                                                                                                                                                  ttttTGGTTCCGCAAAA‑TCTTCCCCAA‑TCCCTCAATAGGCCTCTCATGTAG‑CCGCTTTTGCTGCCCCCACCCGCCCAGTTCAAACGGCGGTTTCTCATGGCTG‑CGCTCTAAACTCAT‑CCCGCGTTTCCGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATTCCATCGCTTCATGCCCGCCAACCGTGGCGTAAatca                                 <  1:1316546/239‑1 (MQ=255)
                                                                                                                                                                                                                  tttGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTCCCAGCACCACTTCCGGCAACAATGCCACGCCAAAGCCGAGTGCCACCATCGATACCATCGCTCCATGCCCGCCAACCGCGGCGCAActc                                  >  2:797332/1‑238 (MQ=255)
                                                                                                                                                                                                                  tttGTGGTTCGGAAAAACTCTCCCATAC‑GGCCT‑AATTGTCGGTCCACGTGA‑CCCCTTTTTCTGCGCCCACCCGCCACGCCTAAACGGGGTTTTCCGCTCGCTG‑CGCTCAAAAATCAT‑CACGCGGTTACGATCCGGTTCGGGGCTGTTTGCCAGCACCACTTCCGGCAACAATGCCCCGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatca                                 <  1:989243/239‑1 (MQ=255)
                                                                                                                                                                                                                  tttGGGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatca                                 <  2:130570/239‑1 (MQ=255)
                                                                                                                                                                                                                   ttGTGGTTGGGCAAAA‑TCTTCCAAAA‑TGCCTCAATTAGCGGCTCATTTAG‑CCGCTTTTTCCGCGCACACACCCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatcat                                <  1:242675/239‑1 (MQ=255)
                                                                                                                                                                                                                    tgtgGTTTCGAAAAA‑GTTTCCCGAACTGTCTCAATTAGGCGCTCCTGTAG‑CCGATTTTTCAGCGCACACCCGCCCAGCTCAAACGGCGTTTTCCAGTCTCTG‑GACTCAAAAATCAA‑CCAGCGGTTACGCACCGGTGCGGGGCTGTTTTCCAGCACCGCTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAatcat                                <  1:94012/239‑1 (MQ=255)
                                                                                                                                                                                                                       ggTTCGGAAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCggg                            <  2:1296127/239‑1 (MQ=255)
                                                                                                                                                                                                                        tttCGGCAAAT‑CTTTCCAGAA‑TCCCCAATTTAGCGCGTCTTTGTT‑CCGCTTTTTCTGCGCACAAACGCCCAGCTCAAACGGCGTTTGCTCATCGCTG‑CGCTCTAAAATCCT‑CCCGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGt                           <  1:731109/238‑1 (MQ=255)
                                                                                                                                                                                                                           cccccata‑TCTTCCCGAA‑AGCCCTAATTAGGCGCTTTTTTTG‑CCGCTTTTTCTGCGCCCACACCCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAACCAT‑CACGCGGTTTCGCCCCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTAc                        <  1:542310/232‑1 (MQ=255)
                                                                                                                                                                                                                           cGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCAGGCCCGCCAACCGTGGCGTAAATCATCgggat                          >  2:278673/1‑235 (MQ=255)
                                                                                                                                                                                                                           cGGAAAAA‑TCTTCCAGAA‑TGCCACAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACGCGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTAc                        <  2:272532/239‑1 (MQ=255)
                                                                                                                                                                                                                                    ttttCCAGAA‑GGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACCACCGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGcca                                                                              <  1:1006818/175‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCCCAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGGGGCGTAAATCATCGGGTGACTGATTTTATTGCGTGGaa      >  1:1276925/1‑239 (MQ=255)
                                                                                                                                                                                                                                               tGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGcca                                                                              >  2:545887/1‑167 (MQ=255)
                                                                                                                                                                                                                                               tGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGcca                                                                              <  1:545887/167‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATc                                                                         <  1:867031/172‑1 (MQ=255)
                                                                                                                                                                                                                                               tGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGCCAACAATGCCACGCCACAGCCGGGTGCCACCATCGATAc                                                                    >  2:1006818/1‑177 (MQ=255)
                                                                                                                                                                                                                                                   tCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACca  <  2:1100761/239‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
TATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGC‑GGGTTAGGGAAAAATGCCTGATAGCGCTTCGC‑TTATCAGGCCTACCCGCGC‑GACAACGTCATTTGTGGTTCGGCAAAA‑TCTTCCAGAA‑TGCCTCAATTAGCGGCTCATGTAG‑CCGCTTTTTCTGCGCACACACGCCCAGCTCAAACGGCGTTTTCTCATCGCTG‑CGCTCTAAAATCAT‑CACGCGGTTACGCACCGGTTCGGGGCTGTTTTCCAGCACCACTTCCGGCAACAATGCCACGCCACAGCCGAGTGCCACCATCGATACCATCGCTTCATGCCCGCCAACCGTGGCGTAAATCATCGGGTTACTGATTTTATTGCGTCGAAACCA  >  NC_000913/3956722‑3957198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑