Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,043,013	A→G	13.6%	G200G (GGA→GGG)	srkA →	Cpx stress response Thr/Ser protein kinase, MazF antagonist protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,013	0	A	G	13.6%	230.5 / 4.4	94	G200G (GGA→GGG)	srkA	Cpx stress response Thr/Ser protein kinase, MazF antagonist protein
Reads supporting (aligned to +/- strand):  ref base A (18/60);  new base G (8/8);  total (26/68)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTAT  >  NC_000913/4042782‑4043244                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            agtggTGGGGCTTTATTTGGGGCGTTGGCACCGAGGGGGGCCAAAACCGCTTTTTATCCACCGCCCAACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGc                                                                                                                                                                                                                                       <  2:739399/235‑1 (MQ=255) aGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑ACTTTACCGTTTTGCGGCCACATGGAGACttc                                                                                                                                                                                                                                       >  2:1048938/1‑236 (MQ=255)    ggTTGGTCTTTATTTAGGGGGTATGCACCGGACGGGGCGCAAACAGCTTTTTATCCAGCCCCCGAACATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATCCCTGCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑Ac                                                                                                                                                                                                                                   <  2:437218/238‑1 (MQ=255)     gTTGGGTTTTATTTGGGGCTGATGCACCAGCCGGGGCTAAAACAGCTTTTTACCCATCGCCCTTCCATG‑GGTTTGAACGAATATCGCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATTCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑GGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGc                                                                                                                                                                                                                                 <  1:775639/239‑1 (MQ=255)      ttGGGCTTTTTTTAGGGCGTTTGCACCAGACGGGGCCAAAACAGTTTTTTATCCTCCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTA‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGc                                                                                                                                                                                                                                 <  2:756958/238‑1 (MQ=255)      ttGGGCTTTATTTAGGGCGTATGCACCAGGCGGGGCGCAAACAGTTTTTTAACCAGCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGc                                                                                                                                                                                                                                 <  2:1211411/238‑1 (MQ=255)      ttGGGCGTTATTTAGGGCGTATGCACCAGGCGGGGCGCAAACAGCTTTTTAACCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGc                                                                                                                                                                                                                                 <  2:166354/238‑1 (MQ=255)      ttGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTCCAGCACACTGGCGGGGAAG‑ATTTAACCGGCTTGGGGC‑ACATGgagagcaccccgcc                                                                                                                                                                                                                                 >  1:287293/1‑229 (MQ=255)      ttGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑AATTTTCCCTTCTGCGGCCACCAGGGGACTGCC‑ACGcc                                                                                                                                                                                                                                >  2:442063/1‑239 (MQ=255)        ggggGTTATTTGGGGCGTAGGCCCCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCgg                                                                                                                                                                                                                              <  2:466230/239‑1 (MQ=255)                tttAGGGCGTATGCACCAGACGGGGCGCAAACATCTTTTTAACCAGCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGAAAGCTGTTTGAGGACGCT‑ACACTGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACg                                                                                                                                                                                                                                  <  2:194524/227‑1 (MQ=255)                     ggCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGCAG‑ATTTCACCGTTTTCCGGCTACATGGGGACTGCC‑CCCCCCGGAGAATtcttttg                                                                                                                                                                                                                 >  2:271377/1‑235 (MQ=255)                          ttGCCCCAGCCGGGGCGCAAACCGTTTTTTACCCATCGCCCGACCATC‑GGTTTGACAGAATATCTCATTGGGCCA‑CGAAAGTTGTTGGAGGCGTCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAACCGACAGT‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGc‑gcg                                                                                                                                                                                                            <  2:966797/237‑1 (MQ=255)                          gtGCACCAGACGGGGCCAAAACAGCTTTTTATCCTTCGCCCGACCATC‑GGTTTGAACAAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGa                                                                                                                                                                                                           <  2:894463/238‑1 (MQ=255)                          agtcaccgagcgggggcaaaaCAGTTTTTTACCCATCGCCCGACCTTG‑GGTTTGAACGAATATCTCATTGAGCCA‑CCCAAGCTGTTTGAGGACGCT‑ACCCTGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGc‑gcg                                                                                                                                                                                                            <  2:663172/220‑1 (MQ=255)                          aTGCACCGGACGGGTCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATTCCATTGAGCCC‑CGCAAGCTGTTTGAGGACGCT‑ACCCTGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGa                                                                                                                                                                                                           <  2:501549/239‑1 (MQ=255)                           tGCCCCAGCCGGGGCGCAAACAGCTTTTTACCCATCGCCCGACCATC‑GGTTTAACCGAATACCCCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGa                                                                                                                                                                                                           <  2:1127967/238‑1 (MQ=255)                               ccAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTTACCCTTCTGCGGCTCCATGGGGACGGCC‑ACGCCGGGGATATTCTCTGGG‑CCggtggt                                                                                                                                                                                                       >  2:374964/1‑238 (MQ=255)                                 aGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑CTTTTCCCGTTCTGCTGCTACATCGAGGCTGCC‑ACGCCCGGGCTATTTTCTGGG‑GGCGTGGTCCa                                                                                                                                                                                                    >  2:51740/1‑239 (MQ=255)                                       ggCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑GATTTACCCTTCTTCGGGTTCATGGGGACTGCC‑CCGCCGGGGAAATtccttgtggcggggggcccatattt                                                                                                                                                                                               >  2:993558/1‑216 (MQ=255)                                       ggCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑AATTTACACTTCTGTGGCCACATCGGGACGGCC‑CCCCCCGGAAGATTCTCTgtcaccagggggcactgttt                                                                                                                                                                                               >  1:255703/1‑220 (MQ=255)                                       ggCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGGAGAATCTCTGGA‑GCGGTGGTCCCAttgtt                                                                                                                                                                                              >  2:1289349/1‑235 (MQ=255)                                         cgcAAACCATTTTTTCACCTTCCCCCGACCTAC‑TGTTTGAAAGAATATCGCAGGGGGCCA‑CGAAATCTGGTTGGGGACGGT‑AAGCTGATCCCTTCCGGGTTG‑AAGG‑CGGCATTCCGAAAAGAGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGATACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAAtgtttg                                                                                                                                                                                             <  1:136833/238‑1 (MQ=255)                                         cgaaaaCATTTTTTTATCCTTCGCCTG‑CCAGCGGGTTGGAACGAATACGTCTTGGAGCAA‑CACAAGGT‑TTTGGGGGCGGTGACAGTGAGCCCGTCGGGGTTG‑AAAG‑CGGCATTCCAGAAGGCGACAGA‑GGAGCTGCTTCCCGCCGTTGCAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAAggtttg                                                                                                                                                                                             <  1:536745/235‑1 (MQ=255)                                         cgaaaaCAGCTTTTTACCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGTACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAAtgtttg                                                                                                                                                                                             <  2:605208/235‑1 (MQ=255)                                         ccaaaaCAGTTTTTTATCCATCCCCCGACCATC‑GGTTTGAACGAATATCTCAGTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACAGTGATCCCTTCCGGTTTG‑AAAG‑CGGCATTCCTGAAAGCGAAAGA‑TGAGCAGATTGCCGCCGGTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAAtgtttg                                                                                                                                                                                             <  2:122522/235‑1 (MQ=255)                                         cataaaCAGTTTTTTACCACCCGCCCCACCGAC‑GTTTTAAACAAATTTTTCATGGGCCCA‑CGCAAGGTGTTTGGGGACCCT‑ACATTGATTCTTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGTTTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAAtgtttg                                                                                                                                                                                             <  1:1256036/235‑1 (MQ=255)                                         caaaaccgcttttttACCCATCGCCCGCCCATC‑GGTTTAAACGAATACCTCATTGGGCCA‑CGCAGGCTGTTTGAGGACGCT‑ACACTGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAAtgtttg                                                                                                                                                                                             <  2:507559/228‑1 (MQ=255)                                          gcAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTTTGTGGCGACATGGAGAGTGCC‑CCGCCGGGGATAATCTCTTGC‑GCGAGCGTCCCAagtttttg                                                                                                                                                                                           >  2:433925/1‑236 (MQ=255)                                                   tttttCTCCTCCGCCCGACCTGG‑TGTTTTAGCGAATTTCTCATTGGGCCA‑CCGCGGGTGTTGGAGGACGCG‑CCATGGATTCCGTCGGGGTTG‑AAAG‑CGGACTTCCGGAAAGGGAAAGA‑TGAGCTGTTTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGgat                                                                                                                                                                                  <  1:1204167/239‑1 (MQ=255)                                                   tttttACCCATCGCCCGACAATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGga                                                                                                                                                                                   <  2:506555/238‑1 (MQ=255)                                                     tttATCCATCCCCCGACTATG‑TTTTTAAATGATTTGCTCGTTG‑GCCACCGAAAGTTGTTTAGGGACGCT‑ACACTGCTTCCTTCGGTGTTA‑AAAG‑CGGCATTCCGTAAAGGGTAAGA‑TGAGCGGATTGCCGCCGTTTCAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGgatga                                                                                                                                                                                <  1:1004166/239‑1 (MQ=255)                                                         cccgccccctctccATT‑GGTTTACACGAATCTCCTTTTGAGCCA‑GACCAGCTTTTTGAGGACGAC‑ACA‑TGAACCCCTTCTGGTTG‑GAAGTCGGCATTCCTGAAAGCAATAGT‑TGGCGTTATTTCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCa                                                                                                                                                                            <  1:374964/227‑1 (MQ=255)                                                            aTCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGCAA‑ATTTCACCGTTCTGCGGCTACATGGGGACTGCC‑ACGCCGGGAAAATTCTCTGGC‑GCGCTGGCCCCATGTTTGTTGATCTGGATGTTGCAcca                                                                                                                                                                         >  2:301235/1‑237 (MQ=255)                                                              cGCCCGACCTTG‑GGTTGGAACGAATTCTTCATTGGGCCA‑CGAAAGCTTGTTGGGGACCTT‑CCCCTGATCCCTTCCGGGTTG‑AAGG‑CGGCATTCCTGAAAGCGACAAA‑TGGGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑a‑a                                                                                                                                                                     <  2:1321647/239‑1 (MQ=255)                                                               gCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑ATTTTACCGTTCTGCGGCTACCTTGGGAATGCC‑CACCCGGGGAAATTTTCTTGG‑GCGGTGGGCCCAAGTTTTTTGTTTTGgaggtttgccgtaaa                                                                                                                                                                      >  2:945724/1‑226 (MQ=255)                                                                      cATG‑GGTTTGAACGAATATCTCATTGAGCCC‑CGAAAGCTGTTGGAGGACGCT‑ACCCGGATCCCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGAAAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAg                                                                                                                                                             <  2:573106/239‑1 (MQ=255)                                                                       ctcgggTTTAAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGGT‑ACATTGATACCTTCCGGTTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATGGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAg                                                                                                                                                             <  2:508128/235‑1 (MQ=255)                                                                       cgtgggTGTGAACGAATTCCTCATGGGGCCA‑CGCAAGCTGTTTGAGGACGTC‑ACACTGATACCTTCCGGTTGG‑AAAG‑CGGCATTCCTGAAGGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAg                                                                                                                                                             <  2:596016/235‑1 (MQ=255)                                                                        cttcttttgatcctctttctttttGGGCCA‑CCCACGCTGTTGGGGGACGCT‑ATCTTGATCCCGT‑CGGGTTGAAAAG‑CGGCATTCCTGAAAGCGACATA‑TGCGCTGATTGCCGACGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTTCATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGc                                                                                                                                                            <  1:392910/217‑1 (MQ=255)                                                                                 aaggattatCCCATGAAGCCA‑GGTAAGCTTTTTGGGGACGCT‑AACCTATTCCTTTCCGGGTTG‑AAAG‑CGGCTTTCCGAAAAGCAAAAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAgg                                                                                                                                                    <  2:729945/232‑1 (MQ=255)                                                                                      atactgcATGGAGCCA‑CGAAAGCTGTTTGAGGACGCT‑ACACTGATCCCTTCGGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTtgt                                                                                                                                              <  2:255703/233‑1 (MQ=255)                                                                                       tatCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑ATTTCACCGTTCTGGGGCTTCAAGGGGCCTGGC‑CCCCCGGGGATATTCTCTTGC‑GCGGTGGTCCCATGTTTTTTGATTTGGATGATTCACCT‑ACATGGTccgccgcgtcggtttttgg                                                                                                                                              >  2:992874/1‑222 (MQ=255)                                                                                             aTTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACCCTGATCCCTTCCGGGTTG‑AAGG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAAtgtttg                                                                                                                                                                                             <  2:97186/187‑1 (MQ=255)                                                                                                   ccA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑CCGCCGGGGATATTTTCTGGC‑GCGCTGGGCCCATTTTTGTTTGTCTTGATGATGCACGTGA‑ATGG‑CCAGCCGGTCAGGATTTGTGGAttgttgtcaa                                                                                                                                 >  2:1269869/1‑230 (MQ=255)                                                                                                               gTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTCCATTGAGGCTGCCAAGGCCGGGAATATT‑TCTGGC‑GCGGTGGGCCCATGTTTGTTGATTTTGATGATGCCCGG‑A‑ATGGTCCCGCCGGTCAGGATTTGTGGTGGTTTTTCCAAGGCGGTaaaa                                                                                                                      >  1:645120/1‑238 (MQ=255)                                                                                                                  tGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGGCCAATGTTTTTTGATCTGGAGGATGCCCGT‑A‑ATGGTCCAGCCGTTCTGGAGTTGTGGGTGTTGCTCAATGGCGCTAAAg                                                                                                                      >  2:558656/1‑236 (MQ=255)                                                                                                                     tgtgctctcacttggtcccTTTCTGGTTT‑AAGG‑GGGCATTCCAGAAGGCG‑GAGAGGGAGGTGTTTGCCGGCGGTACAGCACACTGGG‑TGGAAG‑ATTTCCCCCTTCTGCGGCTAAATTGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATTTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGag                                                                                                                 <  1:1149087/222‑1 (MQ=255)                                                                                                                              cccTGATCCCTTCGGGGTTG‑AAAG‑CGGCATTCCGGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATg                                                                                                        <  2:645120/237‑1 (MQ=255)                                                                                                                                       cccgcgggtttaaaaG‑CGTCATTCCTAAAAGGCAAAAG‑TGTGTTGATTGCCGCCGTTACAGCACGCGGGG‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGa                                                                                                <  1:993558/227‑1 (MQ=255)                                                                                                                                         tgcGGGTGG‑AAAG‑CGGAATTCCAGAAAGCGACAGA‑TGGGTTGATTGCCGGCGTTACAGCACACGGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATAGTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAAc                                                                                             <  2:1020056/237‑1 (MQ=255)                                                                                                                                         tCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGGATATTTTCTGGC‑GCGCTGGTCCAATGTTTGTTGATCTGGATGATGCACGG‑A‑ATGGTCCAGCCGTGCCGGGTTTGTGGATGTTGCTCAATGGCGGTAAAACCGCGCAGCGGATGGAACTGGGAAc                                                                                             >  2:603598/1‑239 (MQ=255)                                                                                                                                         tCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTTGC‑GCGATGGGCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGGTCAGGATTTTTGGGTGTTGCTCCATTGCGGTAAAACCGGGCAGCGGATGGAAATggga                                                                                               >  2:59229/1‑235 (MQ=255)                                                                                                                                           cGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑AATTCACCCTTCTGCGGGTACATGGAGACTGCC‑ACCCCCGGGAAATTTCCTGGCGGCGTGGGCCCATGGTTTGTTGTTTTGGAGGTGGACCGT‑A‑ATGGTCAAGCCGTCAGGGTTTTGTGGTTGTGGTTCATGGGCGGTAAAAcctgctcttttaacaaatgtgcacc                                                                                             >  1:244463/1‑215 (MQ=255)                                                                                                                                                   aaaG‑CGGAATTCCGAAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGa                                                                                    <  2:489838/239‑1 (MQ=255)                                                                                                                                                     aggggggtttCCTGAAAGGGAAGTG‑TGGGCTGATGGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGGGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGa                                                                                    <  2:1237022/230‑1 (MQ=255)                                                                                                                                                         ggCATTCCTGAAAGCGACAGT‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGc                                                                                 <  2:209117/237‑1 (MQ=255)                                                                                                                                                             ttCCTGAAAGGAGAAGG‑TGGGGTGGTTGCCGCCGTTACAGCACACGGGC‑GGGAAG‑ATTTCCGCGTTCTGGGGATACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATga                                                                           <  1:1048938/239‑1 (MQ=255)                                                                                                                                                             ttCCTGAAAGGAGAAGG‑TGAGGTGGTTGCCCTCGTTACGCCCCACGGGT‑GGGAAG‑TTTTCTCCGGTCTGGGGCTACCGGGGGGCTGCC‑ACGCCGGTAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTGGACCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATga                                                                           <  1:51740/239‑1 (MQ=255)                                                                                                                                                              ttctggcacgcgcagaggggggtGATTGCAGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGGCTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATga                                                                           <  2:582259/217‑1 (MQ=255)                                                                                                                                                                     aGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGGAG‑GTTTTACCCGTCAGAGGGAACATAGAGAAGGCC‑ACCCCGGGAACATTTTCTCGG‑GCAAGGGGTCACAGTTTGTTGAGCTCGGTGCGGTACGG‑A‑ATGGTCCAGCCGGTCAGGATTTGTGGGTGTTGTTTACTGAGGATAAAACCGGCGAGCGGATGTCACTGGGAACAattatttgacgttaataaaaaataa                                                                   >  1:767341/1‑220 (MQ=255)                                                                                                                                                                         gtgggtgggtggtttggccacggTTACAGCCGACTGGC‑GGGAAG‑ATTTCGCCGTTTGGCGGCTACAGGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGGTTGTTGATCTGGAGGAGGGTCGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTAGTGAAGCTTATGAAGAATGTAGc                                                                 <  1:301235/216‑1 (MQ=255)                                                                                                                                                                         ggattgtggggtgtttccccccgTTACAGCCCACTGGC‑GGGAAG‑ATTTCCCCGTTCCGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGc                                                                 <  1:59229/218‑1 (MQ=255)                                                                                                                                                                         gaaagtggagtgtaTTGCCGCCGTTACCGCACACTGGC‑GGGAAG‑ATTTCCCCGTTCTGCGGCTACATGGAGACTGAC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGc                                                                 <  2:1099950/225‑1 (MQ=255)                                                                                                                                                                                tggcgtgttGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGa                                                         <  2:287293/232‑1 (MQ=255)                                                                                                                                                                                       ttGCCGCCGTTACAGCACCCTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGc                                                   <  2:811344/238‑1 (MQ=255)                                                                                                                                                                                       ttGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGc                                                   <  2:947108/238‑1 (MQ=255)                                                                                                                                                                                              cgTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACta                                                                                           <  2:170850/191‑1 (MQ=255)                                                                                                                                                                                                ttcccgcccccTGGC‑GGGAAG‑TTTTCACCGGTCTGGGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGa                                         <  1:442063/229‑1 (MQ=255)                                                                                                                                                                                                 tACAGGACCCTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGCCTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCGGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGa                                         <  2:803307/238‑1 (MQ=255)                                                                                                                                                                                                 tACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGa                                         <  2:1213711/238‑1 (MQ=255)                                                                                                                                                                                                 ggagggcgccTGGC‑GGGAAG‑ATTTCCCTGTGCTGGGGTGACGGGGGGGGTGGC‑ACGCACGGAAGAGGCTTTGGC‑GCGAGGGTCAAATGGTTGGTGATCTGGAGGAGGCACGT‑A‑AGGGTCCAGCCGGTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGCGCAGCGGATGCAACTGGAAACTATTATTGAAGCGTAGGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGa                                         <  1:1077680/229‑1 (MQ=255)                                                                                                                                                                                                    aGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGa                                     <  2:1080316/239‑1 (MQ=255)                                                                                                                                                                                                      gcccatggtggggAAT‑TTTTCTCCGTTCTGCGGGTACATTGAGGCTCCC‑ACCGCGGGAATGGTTCCTGGG‑GAGTGGGGCCAATGTTTGGTGGTCTGGATGAAGACAGT‑A‑ATTGTCCAAGCGTTCAGGATTTGTGGATGTTGCTCAATGGCGAGAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTGCGACACCGCTGAAATCGGACGGAt                                    <  1:433925/229‑1 (MQ=255)                                                                                                                                                                                                           tGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTTGC‑GCGATGGTc                                                                                                                                                                                                      >  2:1061354/1‑73 (MQ=255)                                                                                                                                                                                                           tGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTc                                                                                                                                                                                                      <  1:1061354/73‑1 (MQ=255)                                                                                                                                                                                                           ctgggcggAAGAATTTCACTGTTCTCGGGGTGCAGTGAGATGGCC‑ACGTCGGGAATATTTTCGGGC‑GCGATGGGCCATTGTTTGGTGGTCTGGATGATGCACGG‑A‑ATGGTCCAGCCGTTCAGGAGTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAATCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTTATTGaa                                <  1:799803/233‑1 (MQ=255)                                                                                                                                                                                                                 ggAAG‑ATTTCCCCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGTAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTa                          <  2:120746/238‑1 (MQ=255)                                                                                                                                                                                                                       aTTTCCCCGTTCTGGGGCTACATGGGGACTGCC‑CCGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGaa                                <  1:1175415/227‑1 (MQ=255)                                                                                                                                                                                                                         ttCCCCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATgaag                                                                         <  1:549177/184‑1 (MQ=255)                                                                                                                                                                                                                         ttCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATgaag                                                                         >  2:549177/1‑184 (MQ=255)                                                                                                                                                                                                                              tgttgcgCGGGTACTTGGGGTCGGGC‑ACGAGGGGAATATTTGTTGGC‑GCGATGGTCCACTGTTTGTGTATCTGTATCGTGCCCGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGTTAAAACCGAGCAGCGGATGCAACTGGAAACTATTTTTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGACATGCGtt             <  1:802238/233‑1 (MQ=255)                                                                                                                                                                                                                              cgtttggCGGTTTCATGGAGGTGGCC‑ACGCCGGGAATATTCTGGGGC‑GCGATGGTCCAATGGTTTTTGGTCTGGATGGTGCCTGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGtt             <  1:342285/233‑1 (MQ=255)                                                                                                                                                                                                                               gTTCTGGGTCTACATTGGGTTTGCC‑ACGCGGGGAATATTCTCTGGC‑GAGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGttt            <  1:603598/239‑1 (MQ=255)                                                                                                                                                                                                                               gTTCGGGGGCTACATGGGTACTGCC‑CCGCCGGGAATTTTCTCTGGG‑GCGATGGTCCAATGTTTGTTGATCGGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGttt            <  2:98945/239‑1 (MQ=255)                                                                                                                                                                                                                                ttCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGtt                                                            <  2:906357/190‑1 (MQ=255)                                                                                                                                                                                                                                 tCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTAcgcg                      >  2:1175415/1‑227 (MQ=255)                                                                                                                                                                                                                                  tgggggCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGt         <  2:927069/235‑1 (MQ=255)                                                                                                                                                                                                                                  cttgggCTACATGGGGTCTGCT‑ACCCCGGGAATTTTCTCGGGC‑GGGATGGTCCAATGTTTGTTGATCTTGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGt         <  1:1071866/235‑1 (MQ=255)                                                                                                                                                                                                                                  cTGCGGTTACAGTGAGGCTGCC‑AAGCCGGGATTTTTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGt         <  2:619472/239‑1 (MQ=255)                                                                                                                                                                                                                                  cTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGACGCTTATGACGAATTTTGCGAGTTCGACACCCCTGAAATCGGGATGATTGAACCTTTACGCGCCATGCGTTTGGt         >  1:798786/1‑239 (MQ=255)                                                                                                                                                                                                                                         tACATGGAGACTGCC‑ACGCCGGGAATTTTCTCTTGC‑GGGATGGGCCAATGTTTGTTGAACTGGAGGATGGACGT‑A‑ATGGTCCAGCCGTTTAGGATTTGTGGATGTTGCACAATGGCGATAAAGCCGAGCAGCGGATGCATCTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTtattat  <  2:1310256/239‑1 (MQ=255)                                                                                                                                                                                                                                          aCATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTttatta   >  2:833837/1‑237 (MQ=255)                                                                                                                                                                                                                                          aCATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGGTTGGTttatta   >  2:876982/1‑237 (MQ=255)                                                                                                                                                                                                                                          aCATGAAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCACCTAGAAACTATTATTAAAGCTTATGACGAATTTAGCGAGTTCGAAACCGCTGAAATCGGACTGATTGAACCTTTACGCACCAAGAGTTTGGAttatta   >  2:754765/1‑237 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            AGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATC‑GGTTTGAACGAATATCTCATTGAGCCA‑CGCAAGCTGTTTGAGGACGCT‑ACACTGATACCTTCCGGGTTG‑AAAG‑CGGCATTCCTGAAAGCGACAGA‑TGAGCTGATTGCCGCCGTTACAGCACACTGGC‑GGGAAG‑ATTTCACCGTTCTGCGGCTACATGGAGACTGCC‑ACGCCGGGAATATTCTCTGGC‑GCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGT‑A‑ATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTAT  >  NC_000913/4042782‑4043244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑