BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,045,671	A→C	7.9%	*311E (TAA→GAA)	yihG ←	inner membrane protein, inner membrane acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,045,671	0	A	C	7.9%	384.9 / 2.2	148	*311E (TAA→GAA)	yihG	inner membrane protein, inner membrane acyltransferase
Reads supporting (aligned to +/- strand): ref base A (74/55); new base C (2/11); total (76/72)
Fisher's exact test for biased strand distribution p-value = 6.50e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 151 total aligned reads displayed.

CGGAAAAAGCGCAAAAGCAGGCTGATAAACAAATCAGCGGGATGATGACATTAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGTAACATTAAGC  >  NC_000913/4045435‑4045909

    aaaaGCGCAAAAGCAGGCTGATAAACAAATCAGCGGGATGATGACATTAGGTCAGATGGTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTAtt                                                                                                                                                                                                                                            <  2:733298/239‑1 (MQ=255)

         cgcAAAAGCAGGCTGATAAACAAATCAGCGGGATGATGACATTAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAACGAGGAAGAATTTCTGTCTCGTGCCGGACGTTTTTTT‑CATTAAAATACTC‑CTCGCCCTCTCCTCATTCAGATGCGAGTAAAACTACAATATTCTGA‑c                                                                                                                                                                                                                                      >  1:834333/1‑239 (MQ=255)

           aaaagccgggCTGAAAAACAAAGCCGCGGTATGATGACATTAGGTCAAATGTTTCAGTTAATCCCGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑Cg                                                                                                                                                                                                                                     <  2:879246/230‑1 (MQ=255)

           aaaaaGCAGGCTGATAAACAAATCAGCGGGATGAGGACATTAGGTCAGATGTTTCAGTAAATCACGATTG‑AAAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑Cg                                                                                                                                                                                                                                     <  2:607299/237‑1 (MQ=255)

                        aTAAACAAATCAGCGGGATGATGACATTAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGGGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACTTga                                                                                                                                                                                                                      >  2:1235368/1‑239 (MQ=255)

                        aTAAACAAATCAGCGGGATGATGACATTAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTAACTCG‑ACTCTGCTCATTCAGAGGCGAGTAAAACTTACTTAATCTTA‑CCCTGGG‑ATGACAttg                                                                                                                                                                                                                      >  1:181065/1‑237 (MQ=255)

                           aaCAAATCAGCGGGATGATGACATTAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTTGG‑ATGACATgagag                                                                                                                                                                                                                   >  2:249840/1‑239 (MQ=255)

                                aTCAGCGGGATGATGACATTAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAActtactta                                                                                                                                                                                                                                              >  2:763917/1‑209 (MQ=255)

                                        ggagggtgttaaaagggcagggTTTAAGTTAATCACGTTTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGcc                                                                                                                                                                                                       <  2:76065/217‑1 (MQ=255)

                                        gatattgaCATTGGTTCAGATTTTTCAGTTAATCCCGTGTG‑AAAACAATACCGCCTCGCTGCA‑TCTGCGTTAGACACCTGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGcc                                                                                                                                                                                                       <  1:249840/233‑1 (MQ=255)

                                                   tAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATAATCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTCTTCTGA‑CGCTGTG‑ATGTAATGAGAGACGTCAGAAGCTGGGCTTTTccc                                                                                                                                                                                            >  2:423362/1‑238 (MQ=255)

                                                     ggTCATATGTTTCAGTTAACCCCGATTA‑ACAACAATCCCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGc                                                                                                                                                                                         <  2:88185/239‑1 (MQ=255)

                                                        tatgtttttttagtAAACACCATTGAACAACAAGTCCGCCCCGCTGCG‑GCTGCGTTAAACGCGTGGTAAAGTTGTTTTTAACTGACAGGGAGTGAGCAAAGAATATTTTATTGCTGGTGCCGGACGTTTTTTT‑CATTAAAATACTCACTCG‑CCTCTGCCCAGACAGAGGCGAGTAAAACTTCCTTATTCTGA‑CGCTGTA‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGcc                                                                                                                                                                                        <  1:423362/225‑1 (MQ=255)

                                                         aGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACAGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGCGCCGTACGTTTTGGT‑CCTTAAAATACTCACTCG‑CCTCTGCTCATTCGGAGGCGAGTAAAACTTACTTTTCTTTA‑CGAGCGG‑TTGACATTAGAGACCGCAGAAACCGGGCCTTTTccctcccca                                                                                                                                                                                     >  1:60242/1‑232 (MQ=255)

                                                           atttttCAGTTAATCACGATGG‑AAAACAATCCCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTGGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACaa                                                                                                                                                                                    <  2:334321/235‑1 (MQ=255)

                                                           aTGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGTCGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATCCCATGAGAGACGTCCGAAGCCGGCCTTTTTCCTggccccat                                                                                                                                                                                   >  2:341423/1‑232 (MQ=255)

                                                                tCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTCAAACTTACTTATTCTAA‑CCCTGTG‑ATGACATCACAAACCCCAGGATCCGGCCTTTTTCCTGCCCCAATGAGt                                                                                                                                                                               >  2:582196/1‑238 (MQ=255)

                                                                tCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCCGAAGCCGGTCTTTTTCCTGCCACAATGAGtt                                                                                                                                                                              >  2:266056/1‑239 (MQ=255)

                                                                 cAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGCCGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGCCATGAGAGCAGGCAGAAGCCGGTCTTTTTCCTCCCACAATGAGTTc                                                                                                                                                                             >  2:666811/1‑239 (MQ=255)

                                                                    ttAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCCGCGGCGAGTAAAACTTACTTATTTTGA‑CGCTTGT‑ATTGCCTGAGCGACGTGCGACGCCGGTTTTTTTCCTGCCACAAATAGttgaac                                                                                                                                                                          >  1:1021504/1‑235 (MQ=255)

                                                                     tttttttaaggagaaagaattcccGCCTCGC‑GCAGGTTGCGTTATCCACGGGGTAAAGTTTTTTTAAAGGGAAAGGAGAGGAGCGAAAAAGAATTTATTTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTCCTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGc                                                                                                                                                                          <  1:634591/217‑1 (MQ=255)

                                                                      aaccacaATTA‑ACAAAAAGACCGCCTCGCTGCA‑GCTGAGTTATACCCCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCCCGTGCCGGACGTTTTGTT‑CATTAAAATTCTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTCCTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCa                                                                                                                                                                        <  2:1114769/236‑1 (MQ=255)

                                                                      aaTCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGCGGCGAGTAAAACTTACTTATTCTGA‑CGCCGTG‑ATGACATGAGAGACGTCAGAAGCCGGGCTTTTTCCTGCCCCAATGAGTTCAGCCa                                                                                                                                                                        >  2:289683/1‑239 (MQ=255)

                                                                        ccACGATTG‑AAAACAATCCCGCCTCGGTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCAtt                                                                                                                                                                      <  2:1302560/238‑1 (MQ=255)

                                                                         cACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTTCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTCACATACTACCTAC‑CTTCTCCCCCTTCAGAGCGGCATAAAACTTACTAATCTTGC‑CGTCACG‑GTGAACTGGAAGACGACTGAAGCCGGTCTTTTTCTTGcccaaagtcttgtaaccctt                                                                                                                                                                      >  1:943398/1‑220 (MQ=255)

                                                                         cACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCAtt                                                                                                                                                                      >  2:263952/1‑238 (MQ=255)

                                                                                g‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTTAAACTTACTTATTGTGT‑ACCCGTG‑ATGAAATGAGAGAGGGACGAAACCGGGTTTTTTTCTCACACaacaaggtaaaacaatgtagaaaa                                                                                                                                                              >  1:557449/1‑217 (MQ=255)

                                                                                  acaacaATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAACATCCTCACTCG‑CCTCTGCCCATTCAGAGGCGAGTAAAACTTAATTATTCTGA‑AGCTGTG‑GTGAAATGCAAGACGTCAGAAGCCGGTCTTTTTCCTTCCAAAATGAGTTCATCCCTATTTGAAAAt                                                                                                                                                             >  1:125115/1‑239 (MQ=255)

                                                                                   caacaaTCCCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGAGGAGCGAAGAAGAATTTATGTCTCGTGCCGGACTTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATg                                                                                                                                                            <  2:902713/239‑1 (MQ=255)

                                                                                         tACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACCTCAGAATCCGGACTTTTTCCTGCCACAATGAGTTCAGCCAATGCCGAAAATGGCGCtt                                                                                                                                                      >  2:378853/1‑239 (MQ=255)

                                                                                         tACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGACTCGTGCCGGACGTTTTGTT‑CCTTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTCAAACTTCCTTATTCTTA‑CCCTGTG‑TTGACATGAGAGACTGAGGAAGCCGGTCTTTTTCCTTCCACAATACGTTTACCAATTCTTCAAAATgggggtt                                                                                                                                                      >  1:1021780/1‑234 (MQ=255)

                                                                                          aCCGCCTCCCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTa                                                                                                                                                     <  2:972421/239‑1 (MQ=255)

                                                                                              ccTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGCCGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAACCTTACTTATTCTGA‑ACCTGTG‑GTGACATGAAGGACGTCAAAAACCGGTCTTTTTCCTGCCACAAATAATTTAGCCAATGCTACAAAAGGGGGTTaaa                                                                                                                                                   >  1:562962/1‑237 (MQ=255)

                                                                                              ccTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTAATTATTCTGA‑CGCTGTG‑ATGACATTGGAGACGTCAGAAGCCGGGCTTTTTCCTGCCCCCATTAGTTCGGCCATTTCGGAAAATGGCGCTTaaaag                                                                                                                                                 >  1:976952/1‑237 (MQ=255)

                                                                                                tCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAATTTACTTATTTTGA‑CCCTGTG‑TAGAACTGTGAAACGTCAGAAGCGGGGCTTTTTTCTGCCACAATGAGTTTACCAACTTCTGaaatagcggctaaaacgtt                                                                                                                                               >  1:771225/1‑223 (MQ=255)

                                                                                                 cGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGa                                                                                                                                                                                                                                        >  2:604679/1‑151 (MQ=255)

                                                                                                 cGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGa                                                                                                                                                                                                                                        <  1:604679/151‑1 (MQ=255)

                                                                                                 cGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGaa                                                                                                                                                                >  2:634591/1‑221 (MQ=255)

                                                                                                    tGCA‑GTTGCTTTTTCTACCGGTTTAATTTGTTTTTAACGGACAGGATATAGGCGAAGAAAAATTTATGTCTCGTGCCGGACGTTTTGTA‑CATTAAAATCCTCCCTCG‑CCTCTGCTCATTCAGAGGCGAGTAAACCTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGt                                                                                                                                           <  1:344086/239‑1 (MQ=255)

                                                                                                               ttATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTTTCATTGATGTa                                                                                                                                 >  2:420766/1‑239 (MQ=255)

                                                                                                                 ataCACCGGGTTACGGTGTTTTTAACGGACAGTAGATGAGCTAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCGGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGt                                                                                                                               <  2:1128745/239‑1 (MQ=255)

                                                                                                                   acccggggTAAAGTTGTTTTTAACGGAAAGGAAATGAGCGAAGAAGAATTTATGGCCGGTGCCGGACGTTTTGTT‑CATTAAAATCCCCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCg                                                                                                                             <  2:533731/234‑1 (MQ=255)

                                                                                                                   acacCGGGTAAAGTTTTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTTCCGGACGTTTTGTT‑CATTAAAATACTCACGCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCg                                                                                                                             <  2:771225/239‑1 (MQ=255)

                                                                                                                       cGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGGCGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCCGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCCGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTTATGTAGTCGCCAt                                                                                                                         >  2:415366/1‑239 (MQ=255)

                                                                                                                       cGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTAAGAAGCCGGTCTTTTTCCTGCCCCAATGAGTTCAGCCATTTCTGAAAATGGCGCTTAAACCCTTTTTCATTGATTGAgccgcccg                                                                                                                         >  1:1240107/1‑237 (MQ=255)

                                                                                                                         ggTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑Cg                                                                                                                                                                                                                                     >  2:602633/1‑130 (MQ=255)

                                                                                                                         ggTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑Cg                                                                                                                                                                                                                                     <  1:602633/130‑1 (MQ=255)

                                                                                                                         ggTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATAAGTTCAGCCATTGCTGAAAATGGCGCTTCAAGCTTTTGTCTTTGATGTAGTCGCCAttt                                                                                                                       >  2:415172/1‑237 (MQ=255)

                                                                                                                         ggTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAAATTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAAAAACCGGTCTTTTTCCTGCCCAAATGAGTTCATCCCTTGCTGAAAATGGCGCTTAAAGGTTTTGTTATTgttttaatacccccgt                                                                                                                       >  1:745363/1‑224 (MQ=255)

                                                                                                                          gTAAAGTTGTTTTTAACGGACAGGAAATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATCCTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGt                                                                                                                       <  2:1218164/238‑1 (MQ=255)

                                                                                                                                ttttttttgcggtcttggtcaTGGGCGAAGTGGAATTTTTTTCCCGTGCCGGTACTTTTTTT‑CATTAAATGCCGCACCGT‑CCTTGTCTCCTTGAGGGGGGAGTAAAATTTCCTGATTATGA‑CGCTGTC‑CTGCCATGAGGCACGTCCGATGCCTGGCTTTTCCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGGTTTTGTCACTGATGTAGTCGCCATGTAACTCAt                                                                                                                <  1:123739/219‑1 (MQ=255)

                                                                                                                                   tttttAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAAAGGCGATTAAAGCTTTTGTCCTTGATGTAGTGGCCAGTTAAGTCATTgg                                                                                                             >  2:529324/1‑239 (MQ=255)

                                                                                                                                     tttAACGGACAGGCGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑AATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTTAAACTTACTTATTCTTG‑CGCTGTG‑ATTACATTGGAGACGTCAGAAGCCGGACTTTTTTCTCGCCCAATGAGTTTAGCCATTTGTGAAAATGGCGCCTAAACCTTTTGTCATTTATTTATGCTCCATGGAAATCaagggga                                                                                                           >  1:703593/1‑233 (MQ=255)

                                                                                                                                        aaCGGAGAGGAAATGAGCGAAGAATAATTTATGGTTCGTGCCGGACTTTTTGTT‑CATTAAAATCCTCACTGG‑CCTCTGCTCAGTCAGAGGGGAGTAAAACTTCCTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGCCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATa                                                                                                        <  2:181065/239‑1 (MQ=255)

                                                                                                                                        aaCGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGGGCCGGACGTTTTGTT‑AATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATa                                                                                                        <  2:221640/239‑1 (MQ=255)

                                                                                                                                                 gagaTGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGCGAGACGTCAGAAGCCGTTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGCAAATGGCGCTTAAAGCTTTTGTACTTGCTGTAGTCGCCATGTaaacattcggcataagcgtggaaa                                                                                               >  2:155869/1‑217 (MQ=255)

                                                                                                                                                    atggggaaaaaagaaTTTATGTCCGGTGCCGGACGTTTTGTT‑CATTAAAATACCCCCGCG‑CATCTGCTCATGCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGACAGAAGCCGGTCGTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATc                                                                                             <  2:920691/228‑1 (MQ=255)

                                                                                                                                                    aagacaaaaaaagaaTTTTTGTCTCGTGCCGGACGTTTTTTT‑CATTAAAATACCCCCTCC‑CCTTTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGC‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATcc                                                                                            <  1:913103/229‑1 (MQ=255)

                                                                                                                                                    aTGAGCAAAGAAGAATTTAGGTCTCGGGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTGCTTATTCGGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATc                                                                                             <  2:87780/238‑1 (MQ=255)

                                                                                                                                                     tGAGCAAAAAAGATTTTTTGCTTCGTGCCGGACTTTTTTTT‑CATTAAATTACTCACTCG‑CCTCTGCTCATTCAGGGGCGAGTAAAACTTCCTTATTCTGA‑CGCGGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATcc                                                                                            <  1:152751/238‑1 (MQ=255)

                                                                                                                                                      gaccGAACAAGAATTTATTTCTGGTGCCGGACGTTTTTGT‑ACTTAAATTACCCACTCG‑CCTCGGCTCATTCAGAGCCGAGTAAACCTTACTTTTTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCCGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCcac                                                                                          <  2:1247857/236‑1 (MQ=255)

                                                                                                                                                       aGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑CTGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTg                                                                                                                                                                                          >  1:1266966/1‑142 (MQ=255)

                                                                                                                                                       aGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATTAAAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTTAGCCATTGCTGAAAATGGCGTTTAAAGCTTTTTTCATTGTTGTAGTCGCCATGTAACTCATCGGCAAAAGGCTGTAAATCcaaa                                                                                         >  1:378630/1‑237 (MQ=255)

                                                                                                                                                       aGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTg                                                                                                                                                                                          <  2:1266966/142‑1 (MQ=255)

                                                                                                                                                          taagaagaaTTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATCCTCACTCG‑CCCCTGCTCATCCAGAGGCGAGTAAAACTTACTTATGCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACAt                                                                                        <  2:125115/236‑1 (MQ=255)

                                                                                                                                                           aagaagaaTTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACTGCAGAAGCCGGTCTTTTTCCTGCCACAATGGGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCCTTTGTCATTTATTTAGGCGCCCTGAAACTCATGCGAAAAAAGCCTTAAATCCAAATGTa                                                                                     >  1:1162744/1‑239 (MQ=255)

                                                                                                                                                           aagaagaaTTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAAAAGCCTGTAAATCCACATGt                                                                                      >  2:629808/1‑238 (MQ=255)

                                                                                                                                                           aagaagaaTTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGAAGTCAGAAGCAGGTCTTTTTCCTGCCACAAGGAGTTCAGCCATTGCTGAAAAAGGCGCTTAAAGATTTTGTCATTGATGTAGTCGCCCTGGAATTCAACGGAAAAAGATTGGAAATCcccaaata                                                                                     >  1:886946/1‑235 (MQ=255)

                                                                                                                                                                        gTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTCCTTATTCTGA‑CGCTGTG‑ATGACATGTGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAAt                                                                                                                                                                                   <  1:144986/132‑1 (MQ=255)

                                                                                                                                                                             gTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGtg‑a                                                                                                                                                                                                                              <  1:230443/84‑1 (MQ=255)

                                                                                                                                                                              ttcccgggcttttGTTCCTTTAAAATTCTCCCCGC‑CCTCTGCTAATCCAGGGGCGAGTAAACCTTACTTTTTCGGC‑CGCTGGA‑ATGCCTTGAGGGACGTCAAAAGCCGGTTTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGtt                                                                   <  1:420766/230‑1 (MQ=255)

                                                                                                                                                                              tGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGttt                                                                  >  2:1264829/1‑239 (MQ=255)

                                                                                                                                                                                ccgggtgTTTTGTT‑CATTAAAATACTCACTCC‑CCTCTGCTCATGCAGAGGAGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACAGGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTa                                                                 <  2:854463/232‑1 (MQ=255)

                                                                                                                                                                                                    aaaTCCTCCCTCG‑CCTCTGCTCATTCAGAGGCGATTAAAACTTACTTATGCGGA‑CGCTGGG‑AGGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCgaagaa                                             <  2:760559/239‑1 (MQ=255)

                                                                                                                                                                                                                 ccctgcgctatttCAGAGGCAATTAAAACTTCCTTATCCTGA‑CCGTGTG‑AGGACATGAGAGGCGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGtt                                 <  2:703593/228‑1 (MQ=255)

                                                                                                                                                                                                                  ccTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGtg‑at                                                                                                                                                                                                                             <  2:15873/50‑1 (MQ=255)

                                                                                                                                                                                                                  ccTCTGCCCAGTCAGGGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTa                                <  2:557449/239‑1 (MQ=255)

                                                                                                                                                                                                                   ctctGCTCATTCAGAGGCGAGTAAAACTTCCTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATa                                                                                                        <  2:865830/166‑1 (MQ=255)

                                                                                                                                                                                                                      tgctctttagagggggAGTTAAAATTATTTGTTTTGA‑CGATGGT‑AGGACATGGGGGACGTCAGAAGCGGGTGTTTTTCCCGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGt                            <  1:289683/224‑1 (MQ=255)

                                                                                                                                                                                                                      tGCTCATTCAGAGGCGAGTAAATCTTACTTTTTCTGA‑CGCTGTG‑ATAACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATATACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGt                            <  2:834333/239‑1 (MQ=255)

                                                                                                                                                                                                                      ggctattacaggggggtttAAAACTTACTTTTTCTGAGCGTTG‑G‑AGGAAATGAGAGGCGCGAGAATCCGGTCTTTTTCCTGCCACAATTAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGt                            <  1:783460/221‑1 (MQ=255)

                                                                                                                                                                                                                       aaggtttgagagggtaaaaaatCTTTCTTTTTCTGA‑CGCTGGG‑ATGAAAGGAGAGACGTCAGAAGCCGGTTTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGt                            <  1:747717/220‑1 (MQ=255)

                                                                                                                                                                                                                        aagaTTCAGGGGGGAGTAAAACTTTCTTTTTCTGA‑CGC‑GTGTATGACAGGAGAGCGGGCAAAAGCCGTTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGt                            <  1:1168357/234‑1 (MQ=255)

                                                                                                                                                                                                                           aTTCAGAGGCTAGTAAAACTTACTTTTTCTGA‑CGCTGTG‑CGGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCCCATTTAGGACAATCCCCCTCGGTTTACCGCTTAACCAACCGAAAAACGACGGAAGGGATTTGTCCGGa                       >  1:160960/1‑239 (MQ=255)

                                                                                                                                                                                                                             tcggagGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATa                     >  2:774076/4‑239 (MQ=255)

                                                                                                                                                                                                                             tCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCGGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACTCATTAACATACCGAAAGACGGCTGACGGTTATTGTCCggcaa                     >  2:353872/1‑236 (MQ=255)

                                                                                                                                                                                                                             tCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGCTGTAGTCTCCCTGTAACTCATCGGCAAATGGCTGTAGTTCCACATGTACGACAATCCGCTCCAGTTTACCGCTTAACAAATCGAAAAACGCCTGACGGtatatttccagata                     >  1:35730/1‑226 (MQ=255)

                                                                                                                                                                                                                              caaagGCGATGAAATCTTACTTATTCGGG‑CGCTGTG‑ATGACAGGGGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGa                       <  2:942174/233‑1 (MQ=255)

                                                                                                                                                                                                                                  ggggAGTAAATTGTATTTTTTCGGC‑CGCTTGG‑ATGACATCGGAGACATAAGATGCCGGGCTTTGTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAaca                  <  1:837390/234‑1 (MQ=255)

                                                                                                                                                                                                                                  gggaaGTAAACCTTTCTTAATCTAC‑CGCTGTG‑ATGAAGTGAGAGACCTCGAAAGCCGGTCTTTTGCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATTTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAaca                  <  2:284582/233‑1 (MQ=255)

                                                                                                                                                                                                                                  ggCGAGTAAAACTTGCTTATTCTGA‑CGGTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAaca                  <  2:1036158/237‑1 (MQ=255)

                                                                                                                                                                                                                                  ggCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑CTGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGCCATTGGTGTAATCGCCATGTAACTCATCGGCAATAGGCTTTAAATACATATGTAAGACAATCCGCGTCCGTTTACCGATTAACATATCCAGGAAACGCTAAGAGGTATTGTCGCGAAAAcaca                >  1:946826/1‑239 (MQ=255)

                                                                                                                                                                                                                                  ggCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGAAATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAaca                  <  2:813402/237‑1 (MQ=255)

                                                                                                                                                                                                                                  ggCGAGTAAAACTGACTTATCGTGA‑CGTTTGG‑TTCACAAGAGAGACCGCAGAAGCCGGTCTTTTTCCTTCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAaca                  <  2:761305/237‑1 (MQ=255)

                                                                                                                                                                                                                                     gAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAATAACGGCTGACGGTTATTGTCCGGATAacaccggt             >  2:747717/1‑235 (MQ=255)

                                                                                                                                                                                                                                      aGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATaaaa                  >  2:1168357/1‑231 (MQ=255)

                                                                                                                                                                                                                                      aGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGt            >  2:246259/1‑239 (MQ=255)

                                                                                                                                                                                                                                      aGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGt            >  2:1164588/1‑239 (MQ=255)

                                                                                                                                                                                                                                          aaaCTTTATTATTCTGA‑CGCTGGG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGTAACa        <  2:608476/239‑1 (MQ=255)

                                                                                                                                                                                                                                                acttaTTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCTTCAGTTTACCGCTTACCATATCGAACAACGGCTGACGGTTATTTTCCGGAAAAAAAACAGTAACATaaaca  >  1:608476/1‑237 (MQ=255)

CGGAAAAAGCGCAAAAGCAGGCTGATAAACAAATCAGCGGGATGATGACATTAGGTCAGATGTTTCAGTTAATCACGATTG‑ACAACAATACCGCCTCGCTGCA‑GCTGCGTTATACACCGGGTAAAGTTGTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTT‑CATTAAAATACTCACTCG‑CCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGA‑CGCTGTG‑ATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTGAAAATGGCGCTTAAAGCTTTTGTCATTGATGTAGTCGCCATGTAACTCATCGGCAATAGGCTGTAAATCCACATGTACGACAATCCGCGTCAGTTTACCGCTTAACATATCGAAGAACGGCTGACGGTTATTGTCCGGATAACACAGCGTAACATTAAGC  >  NC_000913/4045435‑4045909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`