Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,595	C→A	15.1%	L57L (CTG→CTT)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,595	0	C	A	15.1%	196.0 / 4.0	96	L57L (CTG→CTT)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base C (62/17);  new base A (3/13);  total (65/31)
Fisher's exact test for biased strand distribution p-value = 1.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGG  >  NC_000913/4065357‑4065826                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAG‑GCGG‑TAAAAAA‑CT‑TCGccacc                                                                                                                                                                                                                                          >  2:1235587/1‑239 (MQ=255)   gAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAg                                                                                                                                                                                                                                        <  2:1141864/239‑1 (MQ=255)   acgCAGTTCAACAGACTGTTTGAAAGACCAAACATCATAAACAGCGCGGTTGCAATCGTCGTTTTAACCGGCAACAAAAAGGT‑GGTGGCG‑‑ATAAATTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTGTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCTCCAg                                                                                                                                                                                                                                        <  1:13908/237‑1 (MQ=255)      cAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGaaa                                                                                                                                                                                                                                     >  2:38619/1‑239 (MQ=255)        gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑GGGCC‑CAATATTTTTCCG‑CGA‑GTAGGGTAAAAATCCGGGAA‑GCAAATCGGAAAAC‑GCGG‑TAAAAAA‑CT‑TCGCAACAAAAAAGa                                                                                                                                                                                                                                   >  1:651645/1‑239 (MQ=255)        gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATCTCGGTAAAC‑GCGG‑GAAAAAA‑CT‑TCGCCACCACaaaaa                                                                                                                                                                                                                                   >  1:1141864/1‑237 (MQ=255)        gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCGGAAAGa                                                                                                                                                                                                                                   >  2:697260/1‑239 (MQ=255)        gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGA‑GGAGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACTCCGC‑CGG‑AACTGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGT‑TAAAAAA‑CT‑TCGCCACCATAAAGa                                                                                                                                                                                                                                   >  2:752005/1‑239 (MQ=255)           tattagattttataaatataaaaaCATGATAAACAGGGGTGTGGCTAGCGTCTTTTAAAGCGG‑AAA‑AAAAGGT‑GGGGGGGGAAAAAACGGGGGTG‑TGTAAAATAACCCCGC‑CGG‑AACCGTGTAATAAAAAAAAAA‑G‑GGAGAAATTTTGCTTTT‑AGGCA‑AAATATTTTCCGG‑AGA‑GTCGAGAAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGT‑TAAAAAA‑CT‑CCGCCACCAAAAAGAT‑aa                                                                                                                                                                                                                               <  1:293988/218‑1 (MQ=255)           catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGTGTAAAAATCCGGTGA‑GCATATCGGAAAAC‑GCGG‑TAAAAAA‑AT‑TCGCCACCAAAAAGaa‑aa                                                                                                                                                                                                                               >  1:959432/1‑239 (MQ=255)                   tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAACATAAA‑G‑GGACAAAATTTGCCTTT‑AAGCC‑CAATATTTTTCCG‑CGA‑GTCGAGAAAAAAACCGGGAA‑GCGTAACGGGAAAC‑GCCG‑GAAAAAA‑AT‑TCCCCAACAAAAAGaa‑aacacccaca                                                                                                                                                                                                                       >  1:1304304/1‑231 (MQ=255)                   tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCa                                                                                                                                                                                                                       >  2:935501/1‑239 (MQ=255)                   tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCAGGTAAAAATCCGGTGA‑GCATATCGGAAAAG‑CCGG‑TAAAAAA‑CT‑TCGCAACAAGAAAGAT‑AATCCCGCCa                                                                                                                                                                                                                       >  2:587945/1‑239 (MQ=255)                     tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGATCG‑TTGCAATTAACGCCGC‑CGG‑AACCGATGCGTATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCT‑CGA‑GTCGAGTAAAAAACCGGGTA‑GCATATCGGCAAAC‑ATGG‑TAAAAAA‑CT‑TGCCCAACAAAAAGAC‑aaacaccccaca                                                                                                                                                                                                                     >  1:718950/1‑229 (MQ=255)                     tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTGAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATa                                                                                                                                                                                                                     >  2:330125/1‑239 (MQ=255)                     tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATa                                                                                                                                                                                                                     >  2:293988/1‑239 (MQ=255)                     tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑AAATATTTTTCCG‑CGA‑ATAGAGTAAAAATCCGGTGA‑TCATACCGGAAAAC‑CCGG‑ACAAAAA‑CT‑TCGCCACCAAAAAGAA‑CAACCCGCCaaa                                                                                                                                                                                                                     >  1:338216/1‑237 (MQ=255)                               cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAg                                                                                                                                                                                                                                    >  2:265810/1‑215 (MQ=255)                               cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                           >  2:271177/1‑239 (MQ=255)                               cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                           >  2:716765/1‑239 (MQ=255)                               cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGGGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                           >  2:1177027/1‑239 (MQ=255)                               cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAAAATTTTTCCG‑AGA‑GTCGAGTAAAAATCCGGTGA‑GCAAACCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACAAAAAAGAA‑AATCCCGCCaaaaaaagaagga                                                                                                                                                                                                           >  1:428683/1‑227 (MQ=255)                                 aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGC‑GCATATCGGTAAAC‑GCGG‑GAAAAAA‑CT‑TCGCCACCGGAAAGAT‑AATCCAGCCATAGTAAGCAGGCa                                                                                                                                                                                                          >  2:907809/1‑238 (MQ=255)                                      catAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCACCCCTa                                                                                                                                                                                                    >  2:564390/1‑239 (MQ=255)                                       atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGGGA‑GCATAACGGTAAAC‑GCGG‑TCAAAAA‑CT‑TCGCAACCAGAAAAAA‑AATCCCGcaacacaaaaaaggcaacccaaa                                                                                                                                                                                                   >  1:21196/1‑217 (MQ=255)                                              gcgcgggggccatttcttttttTACCGGGAAAAAAAAGGG‑GGGGGCGA‑ATAAAATGGAGGG‑TTGAAATTACCCCCGC‑CGG‑ACCCGTTGCATAAAAAATAAA‑G‑GGACAAAATTTGCCTTT‑AGGCC‑CATTATTTTTCCG‑CGA‑GTCGATAAAAAATCCGGTGA‑GCATACGGGTAACC‑GCGG‑AAAAAAT‑CT‑TCGCCACAAGAAAAAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc                                                                                                                                                                                             <  1:595067/221‑1 (MQ=255)                                              gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCCGAAAGAT‑AATCCCGCCATAGTAACCAGGCACCCCTAATTCATCg                                                                                                                                                                                            >  2:34859/1‑239 (MQ=255)                                              gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAAtt                                                                                                                                                                                                 >  2:861180/1‑234 (MQ=255)                                              gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                            >  2:458218/1‑239 (MQ=255)                                              gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GAATATCGGTAACC‑GCGG‑TACAAAA‑AT‑TTCCACCCAGAAAGAA‑AATCCCCCCAAAATAAGCAGCAAACCCTAAATcccag                                                                                                                                                                                            >  1:49615/1‑235 (MQ=255)                                              gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAAGATTTTTCCG‑CGA‑GTCGATTAAAAAACCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCACAACGAA‑AATCCCGCCAAAGAAAGAAAGCACACCTaaacaacag                                                                                                                                                                                            >  1:413082/1‑232 (MQ=255)                                               ctcgGTGGCAATCGTCGTTTTAACCGGCAAACAACAGGT‑GTCGGCG‑‑ACAAACTCGAGCG‑TTGCAATTCACGCCGC‑CGG‑ACCCGCTGCCAACAAAATAAA‑G‑GGCCGAAATTTGCCTTT‑AGCCC‑CAAAATTTTTCCG‑AGA‑ATCGGGACAAAATCCGCGGT‑TCATACCGCTAAAC‑CCGG‑AAAAACA‑CT‑TCACCACCCAGCAGAT‑AACCCCGCCATAGAAAGAAggacaccctacatacacgg                                                                                                                                                                                           >  1:1316058/3‑222 (MQ=255)                                               cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGGT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                                           >  2:881549/1‑239 (MQ=255)                                               cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                                           >  2:595067/1‑239 (MQ=255)                                               cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                                           >  2:746514/1‑239 (MQ=255)                                                gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGt                                                                                                                                                                                          >  2:1300143/1‑239 (MQ=255)                                                 cgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCGCCCTAAGTAAGCAGGCACCCCTAATTCATCGGta                                                                                                                                                                                         >  2:501482/1‑239 (MQ=255)                                                   ggtccactcgttgTTTTACCCGGAAAACAAAGTGT‑GGTTGAG‑‑AAAAACTGGACCG‑TTGAAATTAACCCCGC‑CGG‑ACCCGTAGCATAAAAAGTAAA‑G‑GAAAAAATTTTGCCTTT‑GGCCC‑AAATATTTGTCCG‑CGA‑GTCGAAAAAAACTCCGGTGA‑TCATATCGGTAAAC‑GAGA‑TAAAAAC‑CT‑TCTCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat                                                                                                                                                                                        <  1:1300143/231‑1 (MQ=255)                                                           tgttcttttAACCCGAAAAACAAAGGTGGGTGCCA‑‑AAAAAGTGAAGCG‑TGTAAATCAGCG‑CGC‑CGGCACCCGTTGTATATAAAAAAAG‑G‑GGACGAAATGTCTTTTT‑AGGCC‑AAATATTTTTCAC‑AGA‑GTCGAGAAAAATTCCGGTGA‑GCATTCCGGAAAAC‑‑CGG‑TAAAAAATCT‑GCACCACGAGAAAGAA‑AACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                                <  1:752005/234‑1 (MQ=255)                                                            tgttttttAACCGCCAAAAAAAGGGG‑GGGGGCA‑‑ATAAAAGGGAGCG‑TTACAATTACCCGCGC‑AGG‑ACCCGCTTAAAAAAAAAAAAA‑G‑GGAAGAAATTTGGCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑TACGAATAAAAAGCCGGTGA‑AAATATCGTAAAAC‑GTGG‑TAAAAAT‑CT‑TCGCCACCAGAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                              <  1:716765/235‑1 (MQ=255)                                                            gtttttttAACCGCAAAAGAAAATGG‑GGGGGCA‑‑AAAAAATGGAGTG‑GATCAATTACGCCCGC‑CGG‑AACCG‑TGTAAAAAAAAAAAAGG‑GGAAGAATTTGTCGTTT‑AGGCC‑CAATATTTGCCCG‑GGA‑GTCGAATAAAAACCCGGTGA‑TAATATCGGAAACC‑GCGG‑AAAAAAT‑CT‑CCGCCACAAAAAAAAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                              <  1:427868/235‑1 (MQ=255)                                                             ttgtTTTTACCCGGAAAAAAAAGGG‑GGTGGGG‑‑AAAAAAGGGGGCG‑TTGCAAATTACGCCGCGCGA‑AACGGTTGATTAAAAAAAAAA‑G‑GGA‑AAAATTTGGCTTTGAGGCC‑AAATATTTTTGCG‑CGA‑GTGGAGTAAAAACCCGGTGA‑GCTTTTCGGAAAAC‑GCGG‑AAAAAAC‑CT‑TCGCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                              <  1:1257455/237‑1 (MQ=255)                                                              cgtTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTTTAAAATTTgagga                                                                                                                                                                            >  2:361330/1‑239 (MQ=255)                                                                      ccGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgaggagcaaaaag                                                                                                                                                                    >  2:763914/1‑232 (MQ=255)                                                                       cGGAAAACAAAAGGT‑GGT‑GCC‑‑AAAAACTGGGGGGTTTGCAATAAACGCCGC‑CGA‑AACCGCTGAAAATAAAATAAA‑G‑GGACGAATTTTGCCTTT‑AGGCC‑AAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑AAAAAAA‑CT‑TCGCCACAAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACagag                                                                                                                                                                   <  2:206443/239‑1 (MQ=255)                                                                          caaacaaaAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCACAGTAAGCAGGCAACCCTACTTCATCGGAGTAAAATTTGAGGAAATaaaaagaa                                                                                                                                                                 >  1:1179303/1‑231 (MQ=255)                                                                                       ggggggccaaaaacggggggtg‑TTACAATTACCCCCGC‑GGA‑ACCCGCTGATTAAAAAAAAAG‑G‑GTAAGAATTTTGCCTTA‑GGGCC‑CAATATTTCGCCG‑CGA‑GTCGAGAAAAAAGCCGGTGA‑GCATTGCGGTAAAC‑GAGA‑TAAAAAA‑CT‑CCGCCACAAAAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag                                                                                                                                                        <  1:697260/216‑1 (MQ=255)                                                                                        gtggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATCGTAAGAAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCAt                                                                                                                                                   >  2:796883/1‑239 (MQ=255)                                                                                        gtggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATACAATTTGAGGAGATACAGGGTACCGCAGCAGAGCAt                                                                                                                                                   >  2:463469/1‑239 (MQ=255)                                                                                          ggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGa                                                                                                                                                                                                                                       >  1:54502/1‑154 (MQ=255)                                                                                          ggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGa                                                                                                                                                                                                                                       <  2:54502/154‑1 (MQ=255)                                                                                                aTAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTCAGGAGATCCACAGTACCCGTGCCGAGCATTAAAt                                                                                                                                              >  2:1285478/1‑238 (MQ=255)                                                                                                 tAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTc                                                                                                                                            >  2:543928/1‑239 (MQ=255)                                                                                                  aaaCTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCg                                                                                                                                           >  2:901282/1‑239 (MQ=255)                                                                                                        gAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTGAGGAGATACAAAGAACCGATGCAGACAATTAAATTGAAAcca                                                                                                                                      >  1:268499/1‑237 (MQ=255)                                                                                                            ttttgaaataacacccgc‑cgG‑ACCCGCTGAAAAAAAAAAAAG‑G‑GGACAAAATTT‑CCTTT‑TAGCC‑CAAATTTTTTCCG‑GAAGGGCGAGAAAAAACCCGGGAG‑GAATACCGGAAAAC‑GCGG‑TAAAAAA‑CT‑TCCCCACAAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACg                                                                                                                                   <  2:684656/224‑1 (MQ=255)                                                                                                              ttGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGGAATAAAAAGTACCGATGCAGAGAATTAAATTCGAAacgacgac                                                                                                                                 >  1:526885/1‑236 (MQ=255)                                                                                                               tGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCg                                                                                                                                >  2:447570/1‑238 (MQ=255)                                                                                                                        cgccgc‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTAATCGGTATACAATTTGAGGAGATACAGAGTACCg                                                                                                                                                              >  2:891897/1‑199 (MQ=255)                                                                                                                         gccgc‑cGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAACGATGAAGAGAATTAAATTCGAACCGAAGTCCCccaaacaaaa                                                                                                                     >  1:299098/1‑235 (MQ=255)                                                                                                                             c‑cgG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCTAACCGAAGGCCCccaaacaataaggg                                                                                                                 >  1:723233/1‑235 (MQ=255)                                                                                                                                           taaataaaaaaaa‑GAGGAGAAAATTTTCCTTT‑GAGCC‑CAAATTTTTTCCGTGGA‑GTCGAGTAAAAATAGTGTGA‑GCAT‑GCAGAAAAA‑GCGG‑AAAAAAA‑TTCCCGCACCCAAAAAGAA‑ACGCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTccc                                                                                                       <  1:15198/236‑1 (MQ=255)                                                                                                                                            atatAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCa                                                                                                    >  2:15198/1‑239 (MQ=255)                                                                                                                                                aaaaaaag‑G‑GGAAAAATTTGGCCTTT‑AGGCC‑AAATTTTTTCCGG‑GGA‑GTCGAGAAAAAACCCGGGAA‑GAATAGCGGTAACC‑GGGG‑AAAAAAA‑CT‑CCGCCACCAAAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                <  2:268499/239‑1 (MQ=255)                                                                                                                                                aaaaaaaa‑G‑GGAAAAATTTTGCTTTT‑AAGCA‑CATTTTTTTCCCG‑CAA‑TCCAAGAAAAAACCCGAGGA‑ACTACTCGGAAAAC‑GAGG‑AAAAAAT‑CC‑TCCCCACAAAAAAAAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGGCGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                <  1:458218/239‑1 (MQ=255)                                                                                                                                                aaaaaaaa‑G‑GAAAGAAATTTGCCTTT‑AGCCC‑AAATATTTTCCCG‑GGA‑GTCGAGAAAAAATCCGGGGA‑CGAATTGGGTAAAC‑GGGG‑TAAAAAA‑CT‑CCCCCCCAAGAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                <  2:428683/239‑1 (MQ=255)                                                                                                                                                  aataaa‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGAAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGTCGATTTTTTCCCGCAGATTCa                                                                                              >  2:229054/1‑239 (MQ=255)                                                                                                                                                       a‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                                         >  2:624389/1‑239 (MQ=255)                                                                                                                                                            gACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATCCCATAGGCGATTTTTTCCCCCACATTCAGTTTTAAt                                                                                      >  2:1086987/1‑239 (MQ=255)                                                                                                                                                             tgacaatttttctgtttggccacAATTTTTTCCCG‑CGA‑GTC‑AATTAAAATCCGGGGAGGCATCCCGG‑AAAC‑GGGGATAAAAAC‑CC‑ACACC‑CAAAAAAAATAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                                         <  1:348476/215‑1 (MQ=255)                                                                                                                                                              cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATTCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTACCGCCAATTCAGTTTTCATGt                                                                                    >  1:156052/1‑239 (MQ=255)                                                                                                                                                              cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGt                                                                                    >  1:135602/1‑239 (MQ=255)                                                                                                                                                              cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCACGCCGATTCAGTTTTAATg                                                                                     >  2:1083506/1‑238 (MQ=255)                                                                                                                                                                 attttGCCTTT‑ATGCC‑CAATTTTTTCCCG‑CGT‑GCGGAGAAAAAATCCGGGAA‑TCATATCGGAAACC‑GCGG‑TAAAAAC‑CT‑TCGCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAg                                                                                 <  2:413082/237‑1 (MQ=255)                                                                                                                                                                      tgtttt‑GGGCC‑AAATTTTTTCCGG‑GGA‑ATCGAAAAAAAACCGGGGGA‑GAATTGGGGAAAAC‑GCGG‑TAAAAAC‑CT‑CCGCAACAAGAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGAt                                                                            <  2:526885/236‑1 (MQ=255)                                                                                                                                                                      gCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCCTCCCTAATTCATCGGGATAAAATTTGAGAGGATACAGAGTCCCGAGGCAGGGAATACAATTCGAACCGCCTGCGCCCAACCCCTAGGCGTTTTTTTCCCTCAAATTCacatataacataagagaca                                                                            >  1:573614/1‑221 (MQ=255)                                                                                                                                                                       ccTTG‑AGCCA‑CAATTTTTTCCCG‑GGA‑GTCAAAAAAAAATCCGGTGA‑GCATATGGAAAAAC‑GCGA‑AAAAAAC‑CT‑CCCCCACCAAAAAGAT‑ATCCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGAtt                                                                           <  2:21196/239‑1 (MQ=255)                                                                                                                                                                         ttt‑AGGCCACAATTTTTTCCCG‑GAA‑GTGGAAAAAAATCCCGGTGA‑CAATACCGGAAAAC‑GCGG‑TAAAAAC‑CT‑TCCCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTa                                                                          <  2:1304304/239‑1 (MQ=255)                                                                                                                                                                             agccc‑cATTTTTTTCCCG‑GGA‑GTCAATAAAAAATCCGGGAA‑GATTATCGGTAAAC‑GGGA‑AAAAAAA‑CT‑TCCCCCCAAAAAAAAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                      <  2:723233/236‑1 (MQ=255)                                                                                                                                                                             aGGCC‑TAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGCAGATATACAGAGTAACGATGCAGGGCATTAAATTCTAACCGCCGTGGCCCATACCATGGGGGCTTTTTTCCCCGAAATTCCGTTTTAACGTCAAGTGATTATgtg                                                                      >  1:750390/1‑237 (MQ=255)                                                                                                                                                                             aGGCC‑AAATTTTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGGAA‑GAATTCCGGTAAAC‑GCGA‑TAAAAAC‑CT‑TCCCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                      <  2:651645/239‑1 (MQ=255)                                                                                                                                                                                aacaaaTTTTTTCCCG‑GGA‑GTGGAGAAAAAGTCGGGGGA‑GAATATCGGAAAAC‑GGGG‑TAAAAAC‑CT‑CCACCACAAGAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCa                                                                    <  2:1286880/235‑1 (MQ=255)                                                                                                                                                                                           ttCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TAGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATc                                                           >  2:145123/1‑237 (MQ=255)                                                                                                                                                                                              cccgagagcggagtAAAAGTCCGGTGA‑GCATACCGGTAAAC‑GGGG‑TAAAAAC‑CT‑TCACCACCAAAAAAAT‑AACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                                        <  2:1179303/229‑1 (MQ=255)                                                                                                                                                                                                 cga‑gtcgagtAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATATTCACTCCtt                                                    >  2:820610/1‑239 (MQ=255)                                                                                                                                                                                                 cga‑gtcgagtAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGCCGATTTTTTCCCGCAGATTACGTTTTAATGTAAGTGGATTATGGTCAGACATAATCAcctt                                                      >  1:1286880/1‑234 (MQ=255)                                                                                                                                                                                                               actCCGGTGA‑GCATATCGGAAACC‑GGGG‑AAAAAAC‑CT‑TCGCCACCAAAAAAAA‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCa                                       <  2:299098/237‑1 (MQ=255)                                                                                                                                                                                                                    ggTGA‑GTATATCGTAAAAC‑AGGT‑TAAAAAC‑CG‑TCGCCCCCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                                  <  2:349800/239‑1 (MQ=255)                                                                                                                                                                                                                     gTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCACCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCATTTTTAATGTCAGTGGATTATGGTCAGACATAATAACTCCTTATAACGCACCGCAGGGcgt                                 >  2:416397/1‑239 (MQ=255)                                                                                                                                                                                                                      tGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCCCCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt                                                                                                                                                                                  <  2:806756/93‑1 (MQ=255)                                                                                                                                                                                                                      tGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt                                                                                                                                                                                  >  1:806756/1‑93 (MQ=255)                                                                                                                                                                                                                             tgccGGAAAAC‑GCGA‑AAAAAAC‑CT‑CCCCAAGAAGAAAAAG‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                          <  1:1235587/236‑1 (MQ=255)                                                                                                                                                                                                                              atCGGGAAACGGAGG‑AAAAAAC‑CT‑TCGCCACAAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                          <  2:535661/239‑1 (MQ=255)                                                                                                                                                                                                                                     aaC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttattattatt                   >  2:209935/1‑238 (MQ=255)                                                                                                                                                                                                                                       c‑gcgG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTAc                 >  2:322775/1‑238 (MQ=255)                                                                                                                                                                                                                                                          cGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACgg  >  1:223597/1‑238 (MQ=255)                                                                                                                                                                                                                                                          cGCCACCAGAAAGAT‑AATCCCGCCATAATAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGCTTATGGTCAGACATAATCACTCCTTATAACGCCCCCCCGGGCGTCGGTTATTATTATTACCCCCTGCGTTTACgg  >  1:951258/1‑238 (MQ=255)                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         ACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGT‑GGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGG‑AACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAA‑CT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGG  >  NC_000913/4065357‑4065826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑