Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,830 T→G 19.0% V109G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,8300TG19.0% 244.6 / 9.9 123V109G (GTG→GGG) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (25/66);  new base G (22/7);  total (50/73)
Fisher's exact test for biased strand distribution p-value = 5.92e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTT  >  NC_000913/429601‑430066
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGCAACCGGCGGTCAGGTGGTTGGcg                                                                                                                                                                                                                                        >  2:920530/1‑237 (MQ=255)
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGcg                                                                                                                                                                                                                                        >  2:1106067/1‑237 (MQ=255)
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCa                                                                                                                                                                                                                                        >  2:219780/1‑238 (MQ=255)
ctgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGAAGCCATGACATCATGGTCCGGATGCCGCCTGCTGAAGGCGAAACCGGCGGGCCGGGGTTGGGcc                                                                                                                                                                                                                                        >  1:1240085/1‑237 (MQ=255)
 tgTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGGGAAACCGTCGGCATGGGGTGGGGCAg                                                                                                                                                                                                                                       >  2:1049081/1‑238 (MQ=255)
   tttaTCGCTTTTTTCGTTATTATGGGCTTGGGGGGTTTTAACTGGGGGCTGGATTTCACGGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGAAAAACGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCa                                                                                                                                                                                                                                    <  1:1313667/236‑1 (MQ=255)
     aaTCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGGATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAgg                                                                                                                                                                                                                                  >  2:219774/1‑239 (MQ=255)
       ctcgttcgtagcttttttgggggGTGCGTGGCTTTAACTGGGGGGTGGATTGCCCTGGTGGTACGTTTATTAAAATTACCCCCGAAAAACCGGCTGAAATTGCCGTAATGCGTGATGCATTGCAAAAAGCTGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGtt                                                                                                                                                                                                                                <  1:1079506/220‑1 (MQ=255)
          ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGCCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGCGTTGGGCAGCCGGGGTCTg                                                                                                                                                                                                                             >  2:474810/1‑239 (MQ=255)
          ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCAGGTTCTg                                                                                                                                                                                                                             >  2:266733/1‑239 (MQ=255)
          ctgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGAAATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTTGGCAGCCAGGTTCTg                                                                                                                                                                                                                             >  2:155323/1‑239 (MQ=255)
           ttctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGa                                                                                                                                                                                                                            <  2:264498/237‑1 (MQ=255)
           tgctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGCAATCATGGTCCGAATGCCGCCTGCTGAAGGCGAAACCGGCGGCAAGGGGTTGGGCACCCAGGGTCTGa                                                                                                                                                                                                                            >  2:752079/1‑239 (MQ=255)
            ggtATCGTTATTGTGGGCGGTGCGGGTTTTAACTGGGGGCTTGATTTCCCCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGaa                                                                                                                                                                                                                           <  2:1317363/237‑1 (MQ=255)
             ctATCGTTCTTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGAGGCCAGGGTCTGAAg                                                                                                                                                                                                                          >  2:423754/1‑239 (MQ=255)
             ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCCTGGCCCGTATGCCGCCGGCGGAAGGGGAAACCGGGGGACAGGGGTTggggcacacggggttgaaa                                                                                                                                                                                                                          >  1:13968/1‑223 (MQ=255)
             ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTGTGGGCAGCCAGGGTCTGAAg                                                                                                                                                                                                                          >  2:578585/1‑239 (MQ=255)
             ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGGCAGGGGTTGGGCAGCCAGGTTCTGAAg                                                                                                                                                                                                                          >  2:725265/1‑239 (MQ=255)
             ctATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCCGAAGGCGAAACCGCCGGTCAGGGATTGGGCGGCCCGGTCCTGAAg                                                                                                                                                                                                                          >  2:1063364/1‑239 (MQ=255)
                       tatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTTGGAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCCgggttttaacggggttaac                                                                                                                                                                                                                 >  1:1170437/1‑221 (MQ=255)
                          gggCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGCTGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGGTAAAACGGCGGTCACGGGGTGGGGGGCCCGGGTGCGAAGGGGATTAACaaa                                                                                                                                                                                                              >  1:698011/1‑238 (MQ=255)
                          gggCGTGCGCGGCTTTAACGGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAGCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAAt                                                                                                                                                                                                             <  2:803036/239‑1 (MQ=255)
                               gTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGTCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGCGTTGGGCGGCCAGGTTCTGAAGGGGATTAACGAATcca                                                                                                                                                                                                          >  2:1276677/1‑238 (MQ=255)
                                 gcgcGGGTTTAACTGGGGGCTGGATTTCACCGGTGGGACGGTTAATGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATccacc                                                                                                                                                                                                        <  2:383878/238‑1 (MQ=255)
                                 gcgcGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCCGGTTCTGAAGGTGATTAACGAAAccccca                                                                                                                                                                                                       >  2:732504/1‑239 (MQ=255)
                                     gttttttagtGGGGGGTGTTTTTCACGGGTGTGACGGTTGTTGAATTTAGGCGCGAAAACCGGGCGTAAAGTGACGTAATGCGTGATGCATTGCAAAAGGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCGTGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCa                                                                                                                                                                                                   <  1:1114258/230‑1 (MQ=255)
                                     ggttttAACGGGGGGCTGCATTTCACCTGTGGTACGGTTATTGAAATTCCGCCCGAAAAACCGGCTGAAATTGCCGAAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCa                                                                                                                                                                                                   <  2:1111756/236‑1 (MQ=255)
                                        tttaccgggtggtgtgtttgtcccGGTGGTCTGGTTTTTTAAATTCCGGTAAAAAGACCTGCTGAAATGTACGGAATGCGTGTTTCAGTGCAAAAATCCGGCTTTGAAGGTCCGATGCCGCAAAATTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                                <  1:653846/217‑1 (MQ=255)
                                         ttAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                                <  2:1240085/238‑1 (MQ=255)
                                          tAACTGGGGGCTGGATTTCCCCGGTGGGTCGGTTATTGAAATTACGCTCAAAAACCCGGCTGAAATTGACGTAATGGGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                              <  1:743859/239‑1 (MQ=255)
                                          tAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                              <  2:174945/239‑1 (MQ=255)
                                               gggggCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACACCATGGCCCGAATGCCGCCTGCTGAAGGGGAAACCGGCGGTCAGGGGGTGGGCGGGCCGGTTCTGAAGGTGGTTAAAGAAAcccacaacacaaaacagcaa                                                                                                                                                                                         >  1:1194171/1‑221 (MQ=255)
                                                 gggCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCCAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGGGGTCCAGCTTTGGGCAGCCAGGTTCTGAAGGAGCTTAACGGACCCACCCATCAGAATGCACCAGt                                                                                                                                                                                       >  2:1238209/1‑239 (MQ=255)
                                                 gggCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGCCAGGGGTTGGGCAGCCAGGTTCTGAAGGGGGTTAACGAATCCACCAATCCGAATCgagcagg                                                                                                                                                                                       >  2:1076236/1‑238 (MQ=255)
                                                  tgttggttttacctggtggtTAGTTTATTGAAATTCCGCCCGAAAACCCGGCTTAAATTAACTTAAGGCGGGATGCATTGCAAAACGCCGGTTTTCAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                       <  1:1079115/224‑1 (MQ=255)
                                                  ggCTTGATTTCACAGGTGGTGCGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGCCGTAAGGCGTTATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                       <  1:155323/238‑1 (MQ=255)
                                                    gtGGATTTCCCGTGTGGTTGTGTTTTTAAAATTTCGTCTGAAAAACCGGGTGAAATTGCGCGAATGGGTGATTCATGGCAAAAAGCCGGTTTTGAAGAGCCGATGCCGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                     <  1:677038/237‑1 (MQ=255)
                                                     gggATTTCCCCGGTGGTATGGTTATTTAAATTACGCTGGAAAAACCGGCTGAAAGTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGaa                                                                                                                                                                                    <  1:871245/237‑1 (MQ=255)
                                                                   tgtgtgttttgtaaaaTTACGCTCGAAAAACCGGCTGAAATGGACGTAAGGGTGTAGGCATGGAAAAAGCCCGGTTTGGAAGGGCGGTGGCTGCAAAACGTTGGTAGCAGCCATGACATCATGGGCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGGCAGGTGTTGGGCAGCTAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcg                                                                                                                                                                      <  1:1238209/225‑1 (MQ=255)
                                                                   ggtAAGGTTTATTAAATTACGCAAGAAAAACCGGCGAAAATTGACGAAAGCGGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCAATGCTGCAAAACTTTGGTAGCTGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                     <  1:301724/239‑1 (MQ=255)
                                                                        gttttttGAAAGTACGCCCGAAAAACCGGCTGAAATTGAGGTAATGCGTGATGCTTGGAAAAAGGCCGGTTTTGAAGGGCCTGTGCTGCAAAACTTGGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTc                                                                                                                                                               <  1:1076236/234‑1 (MQ=255)
                                                                         gaaaagggacagaCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTc                                                                                                                                                               <  2:227779/226‑1 (MQ=255)
                                                                          tttttGAAATTACGCTCGAAAAACCGGCTGAAAGTTAAGTTATGAGGTATGCATTGCAAAAAGCCGGTTTGTAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCAGGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTTTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTTCg                                                                                                                                                             <  1:1294711/236‑1 (MQ=255)
                                                                           tttggAAATGACGTGCAAAAAACCGGTGGAAATGTCGGTAATGGGAGGTGCATGGAAAAAGGCGGGTTTTGAAGAGTCGAGGCTGCAAAACTTTGGTGGGAGCAATGTCATGATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCg                                                                                                                                                             <  1:531140/234‑1 (MQ=255)
                                                                           tATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAAAATGCAGCAGGGAAGCGGATTGAGTTCGTTGGTCCGa                                                                                                                                                            >  2:619673/1‑239 (MQ=255)
                                                                              tGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGGCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                         <  2:1238729/239‑1 (MQ=255)
                                                                              tGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                         <  2:816333/239‑1 (MQ=255)
                                                                                  aTTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTTGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCCGGGAAGAGTATTGAGGTCGGCGGTCCGAGCGgggg                                                                                                                                                     >  1:287798/1‑239 (MQ=255)
                                                                                     aCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                                  <  2:446039/239‑1 (MQ=255)
                                                                                     aCGCTCGAAAAACCGGCTGAAATTGACGTAAGGCGTGACGCATTGCAAAAGGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                                  <  2:936570/239‑1 (MQ=255)
                                                                                      gatctgAAAAACCTGGGTGAAGTTGACGTTATGGGTGGTGGATTGCAAAACAGGAGTTTGGGGGGGGCGAGGCTGCAAAACGTGGGTAGGAGCGATGGCATCATGGTCCGTATGCCGCATGCTGAAGGCGAAACCGGCGGTCAGGTTTTGGGCAGCCAGGTTATGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGTGTATTGAGTTCGTCGGTCCGAGCGTGGGGGc                                                                                                                                                  <  1:131511/234‑1 (MQ=255)
                                                                                         tCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAc                                                                                                                                              <  2:113311/239‑1 (MQ=255)
                                                                                         acGAAAAACCGGCTGAAATTGACTTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATTCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGACAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAc                                                                                                                                              <  2:657640/238‑1 (MQ=255)
                                                                                                aCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTGTGGGCAGCCCGGTTCTGAAGGGGGTTAACGAATCCACCAATCAGAATGCAGCAGGGAAGGGGATGGGGTTCGTCGGTCCCGACCGggggggggtcctttaaa                                                                                                                                        >  1:227779/1‑228 (MQ=255)
                                                                                                        taaatttgcttAAGGCGTGGTGAATTGAAAAAAGTGGGTTTTGCAGGGCCGATGCGGCAAAACTTTGGTAGCAGCCGTGACATCATGGTCCGGATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAAGGCAGCAGTGAAGCGTATTGGGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTgcgcgcaccag                                                                                                                               <  1:905578/229‑8 (MQ=255)
                                                                                                               acttAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                        <  2:420141/236‑1 (MQ=255)
                                                                                                                         tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGaa                                                                                                                                                                                    <  2:21591/171‑1 (MQ=255)
                                                                                                                         tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGaa                                                                                                                                                                                    >  1:21591/1‑171 (MQ=255)
                                                                                                                             tGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGGATCCACCAATCAGAATGCAGCAGGGACGCGTATTGAGTTCGTCGGTCCGCGCGTGGGGGCGGCCCTGGGGCCAACCGGGGGGGTGGGGTTGATggggg                                                                                                           >  1:944434/1‑236 (MQ=255)
                                                                                                                               gtttcaaaaatGCGGGTTTTGGAGGTGCGATGGCGTCAAACTTTGGTGGGAGCCCTGACAGCATGGTGCGTATGCCGCCTGGTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcg                                                                                                         <  1:740717/233‑1 (MQ=255)
                                                                                                                                aTTGCAAAAGGCCGGTTTGGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                        <  2:1322072/239‑1 (MQ=255)
                                                                                                                                  tGCAAAAGGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTGGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  2:482283/239‑1 (MQ=255)
                                                                                                                                  tGCAAAAAGCCGGTTTTGAAGAGCCGGTGCTGCAAAACTGTGGTAGCCGCCATGACAGTAAGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  1:219780/239‑1 (MQ=255)
                                                                                                                                  tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCCGGTGTTGGGCAGCCAGGTTCTTAAGGGGGTTAAAGAATCCACCAATCAGAATGCAGCAGTGGAGGGTATTGGGTTCGTCGGTCCGGGCGGGGGGGCAGACCTTGGGACAAACGGGtcggtggggtggttggggcgggtg                                                                                                       >  1:16398/1‑216 (MQ=255)
                                                                                                                                  tGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  2:698011/239‑1 (MQ=255)
                                                                                                                                   tgaaaaaGGCGGTTTTGTAAGGGCCGATGCTGCAAAACTTGGGTGGCAGCCATGACAGCAGGGTCCGTATGCCGCCGTCGGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                      <  1:591906/237‑1 (MQ=255)
                                                                                                                                     aaaaaCCGGGTTTTGAGGTGCCCATGCTGAAAAACTTGGGTAGCAGGCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAACCCGGCGGTCAGGTGTTGGGCAGCCAGGTTCCGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGCCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctgct                                                                                                    <  1:1022828/238‑1 (MQ=255)
                                                                                                                                      aaaaGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGGAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGt                                                                                                  <  2:919448/239‑1 (MQ=255)
                                                                                                                                         aGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGGGCGCTGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                               >  2:681564/1‑239 (MQ=255)
                                                                                                                                         aGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGAGGCGCTGCTGTCt                                                                                                >  2:117526/1‑238 (MQ=255)
                                                                                                                                               ttttGAGGAGCGGGTGGTGCAAAACTTTGGTAGCAGCCATTACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                          <  1:117526/238‑1 (MQ=255)
                                                                                                                                                tttggaggggcgtgtgctgaAAATCTTTGGTGGGAGACGTGACATCATGGTCCGTATGCCGCATGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCTAGGTTCTGAAGGTGATTAACGGATCCACCAATCAGAATGCAGCAGTGGAGCGTATTGGGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                          <  1:474810/224‑1 (MQ=255)
                                                                                                                                                          ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAgg                                                                               >  2:158637/1‑239 (MQ=255)
                                                                                                                                                                        cTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                     <  1:609041/141‑1 (MQ=255)
                                                                                                                                                                        cTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                     >  2:609041/1‑141 (MQ=255)
                                                                                                                                                                                  agcCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAATGCCGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGCCCTTGCGCAAACAGGTGCGAGGGCGTTGATGGCAGCGCTGCGGTCTTTCCCCGTGTACGTAGGTTTCGCCttttgagggggacaggg                                                       >  1:338507/1‑225 (MQ=255)
                                                                                                                                                                                    tctggcattctttgTCCGTTGGCCGGCTGCTGAAGGCGAAACGGGGGGTCCGGTTTGGGGCAGCCAGGTTCGGAAGGTGATTAACGAACCCCCCCATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggcgg                                                     <  1:960095/226‑1 (MQ=255)
                                                                                                                                                                                    cgctgtgatcttGGTCCGTTTGCCGCTGGGTGAAGGCGAAACCGGCGGTCAGGGGTTGGGGAGCCCGGTTATGAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggcgg                                                     <  1:200499/232‑1 (MQ=255)
                                                                                                                                                                                      tgttcatcctGGTCCGTATGCCGCCTGGTGAAGGGGAAACCGTCGGTCAGGTTGTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                   <  1:1106067/235‑1 (MQ=255)
                                                                                                                                                                                      aTGACATCTGGGTTCGTAGTGCGGCTGCTGAAGGCGAAACCGGGGGGCAGGTGTTGGGCATCCAGGGTCTGAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                   <  1:579599/239‑1 (MQ=255)
                                                                                                                                                                                         gaataatGGTCGTTATGGCGCCTGGGGAAGGCGAAACCGGCGGTAAGGGGTGGGGCAGACAAGTTCATGAGGTGATTAACGAATCCACCAATGAGAAGGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGTCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                   <  1:748961/231‑1 (MQ=255)
                                                                                                                                                                                          catcagGGTCCGTATGCCGCCTGCTTAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAggg                                                <  2:105934/238‑1 (MQ=255)
                                                                                                                                                                                          catcagGGTCCGTATGCCGCCTGCGGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAggg                                                <  2:331338/238‑1 (MQ=255)
                                                                                                                                                                                              atGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGGGTCCGTAGGTTTCCGCTTTGAGGGGCGACCGGCGGCCGggggggg                                           >  2:790284/1‑238 (MQ=255)
                                                                                                                                                                                                   ccGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACAGATCCACCAATCAGAATGCAGCAGGGAAGGGGATTGAGTTCGTCGGTCCGAACGTGGGGGCAGACCTTGCGCAAAACGGGGCGATGGGGTTGGTGGCAGCGCTGCTGTCTATCCTCGGGGAGGTGGGTTTCCGCTTGGAGAGGGGACTGGGGGCCGgggggggttatg                                      >  1:498683/1‑233 (MQ=255)
                                                                                                                                                                                                    cGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGGGTACGTAGGTTTCCGCTTTGAGGGGCGCATGGCGGCCGGGGGGGTTATTgc                                     >  2:404656/1‑239 (MQ=255)
                                                                                                                                                                                                       aTGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcgc                                   <  2:1194171/238‑1 (MQ=255)
                                                                                                                                                                                                             ccgggtgaagggtgaagcggcggTTAGGTGGTGGGCAGCCAGGTTCGGAAGGTGAGTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTAGTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCa                            <  1:732504/222‑1 (MQ=255)
                                                                                                                                                                                                             ccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTTGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCa                            <  2:287798/239‑1 (MQ=255)
                                                                                                                                                                                                             ccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATAAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTCCGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTTGGCTgggcc                             >  2:180324/1‑235 (MQ=255)
                                                                                                                                                                                                               tgcggAAGGGGAAACCGGGGGTCAGGGTTTGGGGAGCCGGGTTCTGGAGGTGATTAACGAATCCCACAATCGGAATGCAGCAGTGAAGCGTATTGGGGTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGGCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCac                           <  1:619673/238‑1 (MQ=255)
                                                                                                                                                                                                                   tgaGGCGAAACCGGCGGTCAGGTTTCGGGCAGCCAGGTTCGTAAGGTGTTTAACGAATCCGCCAAGCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGGCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  1:332974/237‑1 (MQ=255)
                                                                                                                                                                                                                   gtaGGCGAAACCGTGGGTCGGGTTTTGGGCAGGCTGGTTCTGAAGGGGATTAACGAAACCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCGGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  1:467532/237‑1 (MQ=255)
                                                                                                                                                                                                                   gagtgggAAACCGGGGGTTAGGTGTTGGGCAGGCGGGTTTTGAAGGTGATTAACTAATCCACCAATCAGAATGCAGCAGTGGAGCGTATTGAGTTTGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  1:158637/233‑1 (MQ=255)
                                                                                                                                                                                                                   gAAGGCGAAACCGGTGGTGGGGTGTGGGGATGCCAGGTTCTGAAGGTTAATAACGGATCCACCAATCCGAAGGCAGCAGTGAAGCGTGTTGAGTGCGTCGGTCCGAGCGTGGGGGAAGGCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTTCGTAGGTTTCCGCTTTGAGTCGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                      <  1:920530/239‑1 (MQ=255)
                                                                                                                                                                                                                              cgggggGCAGGGTTTGGGCAGCCAGGTTCTGGAGGTGAGTAACGAATCCCCCAGTCAGGATGCAGCAGTGAAGCGTATTGAGTTCGGCGGTCCGAGCGTGGGGGCAGGCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGc           <  1:1248289/239‑1 (MQ=255)
                                                                                                                                                                                                                                gcggTCAGGTGTTGGGCAACCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTATTTCGCTGGGGCCCGCGGTTAtactttggctg         >  2:1320895/1‑229 (MQ=255)
                                                                                                                                                                                                                                  ggTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATGAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTgg        <  2:655559/238‑1 (MQ=255)
                                                                                                                                                                                                                                        ttgtTGGGCAGGCAGGTTCTGCAGGTGAGTAACGAATCCGCCATATAGAAGGCAGGCGTGGTGGGTATGGAGTTCGTCGGTCCTAGCGTGGGGGCAGACCTTGCGAACACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTTCGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAttt  <  1:266733/237‑1 (MQ=255)
                                                                                                                                                                                                                                         tgtTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAttt  <  2:944434/237‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
CTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTT  >  NC_000913/429601‑430066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: