BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,217,718	T→G	11.0%	V204G (GTG→GGG)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,718	0	T	G	11.0%	314.9 / 2.9	149	V204G (GTG→GGG)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base T (47/78); new base G (14/8); total (63/86)
Fisher's exact test for biased strand distribution p-value = 3.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

AACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATG  >  NC_000913/4217483‑4217954

caccccttcctgcggtccGATCAGATCACATGGCCCGGGGGCATTGAGCCGGGGGGTCCGGGTTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGcc                                                                                                                                                                                                                                            <  1:1166214/223‑1 (MQ=255)

  cgccTTCCGTCGGTGCGAACAGAGCCATGGGTCCGCGGGACGTGAGCCGGGCGATCCGCGATATGTCGATTACTTCCTGCCGATCGTGGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAAGGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCaa                                                                                                                                                                                                                                          <  1:240545/237‑1 (MQ=255)

    ccTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGTGGCAGCGGCAGGTCACTTCGAAGATCAGATTGCGTCAGTGAAGAAATGCGGGCAC‑A‑TGGGCGGCAAAGTTTTAGTGCAAAc                                                                                                                                                                                                                                         >  2:152046/1‑238 (MQ=255)

    ccTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGGTCAGCTGGCGTCAGTGAAGAAATGCGGTCCC‑A‑TGGGCGGCAAGAGTTTAGGGCCAAc                                                                                                                                                                                                                                         >  2:667472/1‑238 (MQ=255)

     cTTCCGTCGCGCCGATCAGATCCAGTGGGCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCGGATCGTTGCAGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGACGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTc                                                                                                                                                                                                                                       <  2:588775/239‑1 (MQ=255)

      ttCCGGCCTTCCGGTCCGATCCAATGGTCCGCGGGCATTGAGCCGGGCTATCCGCGCTATTCCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTATCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGACAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCa                                                                                                                                                                                                                                      <  2:1322585/239‑1 (MQ=255)

       tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGACATGCGGTTAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAg                                                                                                                                                                                                                                     >  2:1008583/1‑239 (MQ=255)

       tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGGGCCAACTccg                                                                                                                                                                                                                                     >  2:711851/1‑237 (MQ=255)

       tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGGGAAGAAATGCGGTCAC‑A‑TGGGCGGCCAAGTTTTAGTGCCAACTCAg                                                                                                                                                                                                                                     >  2:416110/1‑239 (MQ=255)

       tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGGCAGTGAAGAAATGGGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAg                                                                                                                                                                                                                                     >  2:387605/1‑239 (MQ=255)

       tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTAACTTCGAAGATCAGCCGGCGCCAGTGAAGAAATCCGG‑CACAA‑TGGGCGGCAAAGTTTTATGGCCAACCCAg                                                                                                                                                                                                                                     >  2:255316/1‑239 (MQ=255)

        ccGTGGGGCCGATCAGATCCAATGGGCCGGGGGCATGTGGCCGGGCGATCCGCGCTATGTCTATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAgg                                                                                                                                                                                                                                    <  1:1053212/239‑1 (MQ=255)

              tGCCGATCAGTTCCATTGGGCCGGGGGCATTGGGCCGGGCGATCCGGGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                                                                                                                                                                                                                <  1:592580/237‑1 (MQ=255)

                        aTCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTATGGCCAACTCAGGAAGCTATTCAGaaa                                                                                                                                                                                                                      >  2:592580/1‑237 (MQ=255)

                        aTCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAACGCCTTTGAACTGATGAAA‑GCGGTGATTGAAGCCGGTGCCGCGGCAGTTTACATCAGAGATCACCTGGCGTCAGTGAAGAAATGCGGACAC‑A‑GGGGGGGAAAAGGTTTTGGGCCaaacacgaaaaggatttagaaaag                                                                                                                                                                                                                    >  1:1250058/1‑217 (MQ=255)

                        aTCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGAGGAAGCCGGTTTTGGCGGTGTCCTGAATGACTTTTAACTGATGAAA‑GCGATGATTGAAGCCGGCGCCGCGGCCGTTACCTTCGAAGATCAGCCGAGGTCCGTGGGGCAATGAGGGCAA‑C‑AGTGCGGCGAAGTTTTCATGCCAACACAGAAAGCCATTcgcaaaaa                                                                                                                                                                                                                    >  1:392175/1‑232 (MQ=255)

                              tGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTTCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGCCGGCAAAGTTTTAGTGCCACATCCGGAAGCTATTCGGAAACGGGTcgc                                                                                                                                                                                                              >  2:1117684/1‑239 (MQ=255)

                               ggTCCGGGGGTGTTGGTCCTGGCGGTCCGCGGTATGGTGATGACTTCCTGGCGATCGTTGCCGATGCTGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTcgc                                                                                                                                                                                                              <  1:1082811/238‑1 (MQ=255)

                                  ccGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTTGTCgcggc                                                                                                                                                                                                           >  2:1223335/1‑238 (MQ=255)

                                  ccGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGGGCCAACTCAGGAAGCTATTCAGAAACTGGTCgcggcg                                                                                                                                                                                                          >  2:276225/1‑239 (MQ=255)

                                   cgcgGGCATTGAGCCGGGCGATCCGCTCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGGGGCAGTTCACTTCGCAGAACCGCCGGCGTCAGTCACGTAATGCGGTACC‑A‑CGGGCGGGAAAGGTTTCGAGCCAAATCAGGGAGCTATTACCAAAAGGggcgcggcgc                                                                                                                                                                                                         >  1:831870/1‑239 (MQ=255)

                                   cgcgGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCCGAAAATGGTCCGGgcgc                                                                                                                                                                                                         >  2:560082/1‑239 (MQ=255)

                                   cgcgGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGGGCCAACTCAGGAAGCTATTCAGAAACTGGTCgcggcgg                                                                                                                                                                                                         >  2:411990/1‑238 (MQ=255)

                                   cgcgGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGGGTCCTGAATGCCTTTGAACTGCTGAAA‑GCGATGATTGAAGCCGGGCCCACGGCAGTTCACTTCGAAAGTCAGCTGGCGTCATGTAAGAAATGGGGGCAC‑A‑TGGGCGGGAAACGTTTAGGTCCAACCCAGGAAGGTAtttgaaaaacggcgcgcgccc                                                                                                                                                                                                         >  1:52526/1‑220 (MQ=255)

                                   cgcgGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGAGGCGGAAGCCGGTTGTGGCGGTGTCCTGAATGCCTTTGCACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGCAGATCAGCTGGGGTCCGTGCGAAAATGTGGTCCA‑A‑CAGGCGGCAAAGTTTTTAGGCCAACACAGGGACAAATTTAGAAACTGGACgcggcgg                                                                                                                                                                                                         >  1:469256/1‑238 (MQ=255)

                                                   ggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGCAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCCAAGATCGGCTGCCGTCAGTGCAGGACTATGGTCCC‑A‑TGGGGGCCAAAGTTTTATGGCCCCCCCACGAAACGATTCCAAAACTGGTcgcgcgcggctctgggcctacagg                                                                                                                                                                                         >  1:1317419/1‑219 (MQ=255)

                                                   ggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGGTGATTGAAGCCGGTGAAGCGGCAGTTCACTTCGGAGATCACCCGGCGTCAGTGAGGAAATCGGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCACGCCAGACAGGTATTCAGAAACTGGTCGCCGCGCGTGTCGCAGGtgtgga                                                                                                                                                                                         >  1:924061/1‑235 (MQ=255)

                                                   ggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGCAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGCCGCGGCGGGTCTGGCAGcgc                                                                                                                                                                                             >  2:988786/1‑233 (MQ=255)

                                                        tCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAC‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGACGAAATGCGGTGAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCCGGAAGCTATTCAGCACCTGGTCGCGGCGAGGCTGGCAGCTG‑ACGTGACgg                                                                                                                                                                                   >  2:326677/1‑239 (MQ=255)

                                                        tCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCCGAAACTGGTCCGGGCCCGTCTGGCACCTG‑CCGGCACgg                                                                                                                                                                                   >  2:433353/1‑239 (MQ=255)

                                                        tCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGCCgg                                                                                                                                                                                   >  2:444285/1‑239 (MQ=255)

                                                         ccggtgtttGTTGATTACTTCCTGCCGATTGTTGCCGGTGCGGAAGCCGGTTTTGGCGGTTGCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGGCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACggg                                                                                                                                                                                  <  1:433353/231‑1 (MQ=255)

                                                             gcTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTGGGCGGTGTCCTGAATGCCTTTGAACTTATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAGGATCCGCTGGCGTAACTGAATAAATGGGGTCAC‑A‑CGGGCGGCAAAGTTTTAGGTCCAACTCAGGAAGCTATTCGTAAACTGGTCGCGGCCAGACTGGGACCCG‑CCGTGACCGGCGt                                                                                                                                                                               >  1:990536/1‑238 (MQ=255)

                                                                  gTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTACA‑A‑TCGGCGGCCAAGTTTTAGTGCCAACTCAGGAAGCTCTTCAGAAACTGGTGGCGGCGCGCCTGGCACCCG‑CCGGGACGGGGGTTCCac                                                                                                                                                                          >  1:834102/1‑237 (MQ=255)

                                                                       ttACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGCAGATCAGCTGGCGTCCGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTATTGCCACCCAGGGAAGCCATTCAAAAACGGGTCCCACCGCGTCTGGCAGCAT‑CCGTAACGGGCTTTCCACCCctcc                                                                                                                                                                    >  1:58041/1‑237 (MQ=255)

                                                                          tttccttcagtttgtggCCGGTGGGGAATCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGAAGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTgg                                                                                                                                                                 <  1:416110/223‑1 (MQ=255)

                                                                           ttCCTGCCGATCGTTGCCGCTGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGGTCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAACGTGTTAGGGCCAACTCAGGAAGCTATTCAGAAACTGAGGGCGGGGCGTCGGGGGGGCG‑ACGGGGCGGGGGTACCAAACctgtgggg                                                                                                                                                                >  1:1136103/1‑234 (MQ=255)

                                                                           ttCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGt                                                                                                                                                                >  2:436697/1‑239 (MQ=255)

                                                                             ccTGCCGATCGTTGCCGATGCGGAAGCCTGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTAACTTCGAAGATCAGATGGCGTCAGGGAAGAAATGCGGCTAC‑A‑TGGGCGCCAAAGTTTTAGTGCCAACTCAGGGAGCTATTCAGAAACTGGTCGCGCCGCGCCCGGCAGCTG‑ACGTGCAGGGCGGTCCAAACctgcgtgtg                                                                                                                                                               >  1:1309725/1‑233 (MQ=255)

                                                                               tGCCGATCGTGGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCGGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGcc                                                                                                                                                            <  2:519196/239‑1 (MQ=255)

                                                                               ggccgggctttGCCGTGGCGGAAGCGGTGTTTGGCTGTTTCTTGAAGGCCTGTGAACTGAGTAAA‑GCGATGATTGAAGCCGGTTCAGCGGCGGTTCCCGTCGAAGGTCAGCTGGCTGCAGTGAAGAAATGCGGTCCC‑A‑TGGGCGGCAAAGGTTTAGTGCCAACTCAGGAATCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGcc                                                                                                                                                            <  1:1073882/230‑1 (MQ=255)

                                                                                             gtggCGGAAGCGTGTTTTGGGGGTTTCCTTGATGCCTTGGAACTGATGAAA‑TGGTTGGTTGAAGCCGGTGCCGCGGCAGTTCACTGCGAAGATCAGGTGGCGGCAGTGAAGAAAGTCGGTCAC‑A‑TGGGCGGCAAAGTGTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑CCGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGa                                                                                                                                              <  1:728021/236‑1 (MQ=255)

                                                                                                  gggaagctgTTTTGGGGGTGTCATGAATGCCTTTTGACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGGGGAAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                                                         <  1:307018/231‑1 (MQ=255)

                                                                                                   gagggcGGTTTTTGGGGTTGCCTTAATGCCTTGGAACGGAGGAAA‑GGGGTGATTGAAGCCGGGGCAGCGGCAGTTCACTTCGAAGAGCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACGCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                                                         <  1:680818/233‑1 (MQ=255)

                                                                                                     atgcGGGTTTGGCGGTTTTCTTATTGCCTTTGAACTTATGAAAGGTGGTG‑TTGAAGCCGGTTCAGCGGCATGTACCTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑GGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcggcg                                                                                                                                       <  1:326677/235‑1 (MQ=255)

                                                                                                         ggTTTTGGCGGTGTCCTGAATGCCTTGGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCt                                                                                                                                  <  2:156878/239‑1 (MQ=255)

                                                                                                          gTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCCCTTCGAAGATCTGCTGGCGCCAGTGACGACATTCGGTCAC‑A‑TGGGCGGCAAAGTTTTAATGCCAATCCAGGATGCTATTCAGAAAGTGGTCGCGGACCGTCCGTAAGCCG‑ACGCGTCGCACGCTTCCACCCCCCAGGTTTCCCCTACCGCCCGCGATGCGGGGGATcc                                                                                                                                  >  1:512711/1‑237 (MQ=255)

                                                                                                          gTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGGGGTCCC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTGCGTCAACTGAACGT‑ACGGGCGGTCCCACCCCGCCGGTGGCCCGGTCCGATGGTGTAGCGGGGGACCTg                                                                                                                                 >  1:885963/1‑239 (MQ=255)

                                                                                                             ttGGCGGGTTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGGGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATc                                                                                                                              <  1:950167/239‑1 (MQ=255)

                                                                                                                   gtgtCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAGGTTTGAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGGGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTc                                                                                                                         <  1:260789/238‑1 (MQ=255)

                                                                                                                   gtgtCCTGAAGGCCTTGGAACTGATGAAA‑GGGAGGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTc                                                                                                                         <  2:840583/238‑1 (MQ=255)

                                                                                                                     gtCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGGGCTGagaccccccgg                                                                                                                      >  2:459347/1‑229 (MQ=255)

                                                                                                                          taaTGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGGAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                                                                 <  2:76070/238‑1 (MQ=255)

                                                                                                                          gAATGCCTTTGAACTGATGAAA‑GCGATTGTTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                                                                 <  2:442865/239‑1 (MQ=255)

                                                                                                                           aaTGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGGGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGGGCGTCTGGCAGCTG‑ACGGTCAGGGGGTTCAAACCCGCCCGGTTGCCGGGACCCAGGCGGAGGcggggggagctattcacccccgtgggg                                                                                                                 >  1:1126074/1‑217 (MQ=255)

                                                                                                                            aTGCCTTGGAACGTATGAAA‑GCGAAGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGAc                                                                                                               <  2:499795/239‑1 (MQ=255)

                                                                                                                                cTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGAACTGGTTACCTCCGATTCCGAccc                                                                                                             >  2:816381/1‑237 (MQ=255)

                                                                                                                                  ttGAACTGATGAAA‑GCGATGATTGAAGCCGGTTCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAg                                                                                                                                                                                                <  1:774667/153‑1 (MQ=255)

                                                                                                                                  ttGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAg                                                                                                                                                                                                >  2:774667/1‑153 (MQ=255)

                                                                                                                                  ttGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCCATTGCGACCCGTAt                                                                                                         >  2:1031284/1‑239 (MQ=255)

                                                                                                                                        tgatgaAA‑GCGATGATGGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAACTATTCAGAAGCGGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGGCGGGGGTTCCAAGCCTGAGGGTAGGGAAATCCGATGGAGATGAGGCGGATCGGATCACCTCCGATTGCGACCCGTATGACAGc                                                                                                   <  2:1070025/239‑1 (MQ=255)

                                                                                                                                         gatgaAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCg                                                                                                  <  2:762994/239‑1 (MQ=255)

                                                                                                                                          atgaAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGCTGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTAttaaacgga                                                                                                 >  2:6499/1‑231 (MQ=255)

                                                                                                                                                      gggttgagcggggtccgggggaGTTCACTGCGAAGAGCGGGTGGCGGCAGTGAAGAAATGCGGTCAC‑A‑GGGGGGGCAAAGTTTTAGTGCAAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCg                                                                                      <  1:711851/218‑1 (MQ=255)

                                                                                                                                                      gatTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCg                                                                                      <  2:1210676/239‑1 (MQ=255)

                                                                                                                                                        ttgagtcgggTGCAGTGGCAGTTGCGGTCGAAGATCGAGTGGGGTCAGTGAAGGAAGGCGGTCAA‑A‑TGGGGGGCAAAGTTTTAGTGCAAACCCAGGAAGCTATTCAGAAACTGGTCGGGGGTCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCggt                                                                                    <  1:912500/231‑2 (MQ=255)

                                                                                                                                                                 gTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGAGGCTGATGCGGCGGATCTGATCACCTCCGGTTGCGACCCTTTTTAACGGGGATTTATTTCCGGCggaggcacc                                                                           >  2:1195852/1‑231 (MQ=255)

                                                                                                                                                                        ggCAGTTCACTTCGAAGAGCAGGTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTg                                                                       <  2:775274/236‑1 (MQ=255)

                                                                                                                                                                           aGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                                                  <  2:502980/238‑1 (MQ=255)

                                                                                                                                                                           aGTTCACTGCGAAGATCAGCTGGCCTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                                                  <  2:369416/238‑1 (MQ=255)

                                                                                                                                                                               tatttggagtgttggcgggCGTGAGTGAAGAAATGCGGGCAA‑A‑TGGGGGGCAAGGTTGTAGTGACAGTGCAGGAAACTATTCAGAAACGGGTCGCAGCGCGTGTGGAAGCTG‑ACGGAGCGGGCGTTCCAACCCTGCTGGTTGCCCGTACAGATGCTGATGCTGCGGATCTGATCACCTCCGATTGCGACCCGAATGACAGCGAATTTATTACCGGCGAGCGTCCCAGTGAAGGcttctt                                                             <  1:1008583/222‑1 (MQ=255)

                                                                                                                                                                               cctctccaaGGTCAGGTGGCGTCCGGGAAAGAATGCGG‑GAC‑ATTGGGGGGGAAAGTTTTGGGGACAAGTCAGGAAGCTATTCAGAAACTGGTCGTGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGcttctt                                                             <  1:1215350/232‑1 (MQ=255)

                                                                                                                                                                               ccattCGAAGGTCAGCTGGCGTCAGTGAAGAAATGCGGTCCC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGcttctt                                                             <  2:52526/236‑1 (MQ=255)

                                                                                                                                                                                 cgtCGAAGATCAGCGGGCGTCAGGGAAGAAAGGCGGTAAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTcc                                                           <  2:10329/237‑1 (MQ=255)

                                                                                                                                                                                  gtCGAACAGCAGCTGGGGTCAGTGAACAAATGCGATCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACCCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCGGGCAGCTG‑ACGGTACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCg                                                          <  1:805669/238‑1 (MQ=255)

                                                                                                                                                                                          tttgccggctgtaGTGAAGAATTGCGGTCCC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATg                                                  <  1:276225/227‑1 (MQ=255)

                                                                                                                                                                                            aGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGGGCGTACCAGGGAAGGCCTCTTCCGTACTCAAGCg                                                >  2:1307943/1‑239 (MQ=255)

                                                                                                                                                                                                      tgTGGAGAAATGCGGTCCC‑A‑TGGGGGCGAAATTTTTAGTGGCAACTCAGGAAGCTATTCAAAAACTCGTCCCGGCGCGTGTGGCAGGTT‑ACGTGGCGGGCGTTCCAACCCGGCTGGTTGCCCGTACCGAGGCTGATGCGGCGGATCTGATCACCGCCGATTGGGACCCGTATGACAGCGAATTTATTACCGGCGTGCGTTCAAGTGAAGGCTGTTCCCGTATCCATGCGTCCATTGAGc                                      <  1:988786/238‑1 (MQ=255)

                                                                                                                                                                                                      aGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTGAGGAAACTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAg                                       <  2:1317419/238‑1 (MQ=255)

                                                                                                                                                                                                       gTGAAAAAAGGCGGTAAC‑A‑AGGGCGTGAAGGTTTTAGGTCCAACCCAGGAAAGTCGTGAGAACCTGGGCGCGGCCGCTCTGGCACCGG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCCGATCTGATCACCTCCGAGTGGGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGc                                      <  1:560082/238‑1 (MQ=255)

                                                                                                                                                                                                        tGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTg                                                                       <  2:189562/204‑1 (MQ=255)

                                                                                                                                                                                                         gaagaaATGGGGTCAC‑A‑GGGGGGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCaa                                    <  2:530969/238‑1 (MQ=255)

                                                                                                                                                                                                             taaTTCGGTCAC‑C‑TTGGGCGCAAAGTTTTGGTGGCAACCCAGGGAGGTTTTTAGAAACTGGGCGCGGCGGGTTCGGCAGCTG‑ACGGGGCGGGCGTTCCAACCCTGCCGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGAt                               <  1:1195539/238‑1 (MQ=255)

                                                                                                                                                                                                              aaTGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGAt                               <  2:1309350/238‑1 (MQ=255)

                                                                                                                                                                                                                  cGGTCCC‑A‑TGGGGGGCAAAGTGTTAGGCCCACCTCAGGCATGAATTCAGAACCTGGCCCGGGGGGGTCGGGCAGCTG‑GCGGGACGGGCGATCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGCTCCCCTCCGATTGCGACCCGTCTGACAGCGAATGTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGcc                          <  1:152046/239‑1 (MQ=255)

                                                                                                                                                                                                                   ggTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGcc                          <  2:978433/238‑1 (MQ=255)

                                                                                                                                                                                                                      gtgccgtGGGGGGAAAGGTTTTGGTGCCAACTCAGGAAGTTTTTCTGACACGGGGCGGGGTGCGTTGGGCGGCTG‑ACGTGCCGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGt                        <  1:393382/233‑1 (MQ=255)

                                                                                                                                                                                                                                   cacaGTTTTAGGGCCAACCCAGGAAGCTTTTCGGAAATGGGCCGCGGCGCGTCTGGCAGGTG‑ACGTGACGGGCGTTCCAACCCGGCTGTGTGCCCGTACCGATGCTGATGCGGCGGATCTGATCCCCTCCGATTGCGCCCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt            <  1:1031284/235‑1 (MQ=255)

                                                                                                                                                                                                                                   cAAATTTTTAGTGCCAACGCAGGACGCTCTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGG‑ACGTGCCGGGCGTTCCAACCCTGCGGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt            <  1:1053162/238‑1 (MQ=255)

                                                                                                                                                                                                                                   cAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGCTCTGATCACCTCCGATTGCGGCCCGTATGACAGCGAATTTATTACCGGCGATCGTACCAGGGAAGGCTTCTTCCGTACTCATGCGGGCCTTGTGCAAGCGATCAGCCGTGGCCTGGCGTAt            >  2:129712/1‑238 (MQ=255)

                                                                                                                                                                                                                                   cAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt            <  2:1069010/238‑1 (MQ=255)

                                                                                                                                                                                                                                   cAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACGGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt            <  2:11173/238‑1 (MQ=255)

                                                                                                                                                                                                                                   cAAAGTTTTAGTGCCAACCTAGGAATCTATTCAGAAACTGGTCGCGGCGGTTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt            <  2:1266722/238‑1 (MQ=255)

                                                                                                                                                                                                                                    aagtttttAGTGCCAACTCAGGAAGCTAGTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGT‑ACGGGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATg           <  2:1024675/234‑1 (MQ=255)

                                                                                                                                                                                                                                    aaaGTTTTTGTGCCACCGCAGGAAGCTATGCAGAAACTGGGCGGGGCGCGTCGGGCAGCTG‑ACGTGACGGGCGGTCCAACCCTGCTGGTTGCCCGTACCGAGGCTGATGCGGCGGATCTGATCAACTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATg           <  1:6499/238‑1 (MQ=255)

                                                                                                                                                                                                                                         tttAGTGTCAACTCAGGAAGCTAATCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc       <  2:990536/237‑1 (MQ=255)

                                                                                                                                                                                                                                         tttAGTGCCCACCCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc       <  2:703534/237‑1 (MQ=255)

                                                                                                                                                                                                                                         tttAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc       <  2:1136103/237‑1 (MQ=255)

                                                                                                                                                                                                                                         tttAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc       <  2:901437/237‑1 (MQ=255)

                                                                                                                                                                                                                                            aGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCCCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCGCCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATg  <  2:469256/239‑1 (MQ=255)

AACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCAC‑A‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATG  >  NC_000913/4217483‑4217954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`