BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,217,811	C→G	4.5%	A235G (GCT→GGT)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,811	0	C	G	4.5%	416.6 / 2.4	131	A235G (GCT→GGT)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base C (32/89); new base G (4/5); total (36/95)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAATATCCGGGCAAACTGC  >  NC_000913/4217576‑4218047

gtggCGGAAGCGTGTTTTGGGGGTTTCCTTGATGCCTTGGAACTGATGAAA‑TGGTTGGTTGAAGCCGGTGCCGCGGCAGTTCACTGCGAAGATCAGGTGGCGGCAGTGAAGAA‑AGTCGGTCACA‑TGGGCGGCAAAGTGTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑CCGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGa                                                                                                                                                                                                                                            <  1:728021/236‑1 (MQ=255)

gATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGa                                                                                                                                                                                                                                            <  2:145067/239‑1 (MQ=255)

     gggaagctgTTTTGGGGGTGTCATGAATGCCTTTTGACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGGGGAAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                                                                                                                                                       <  1:307018/231‑1 (MQ=255)

     ggACGCCGGTTTGGGCGGGATACAGAATGCCTTTGAACTGAGTAAA‑GCGATGATTGAAGCCGTGACAGCGGCAGTTCGCTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGGGGCAAAGTTTTAGTGCGAACTCAGGAAGCTATTCAGCAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTGCCACCCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                                                                                                                                                       <  1:1034856/239‑1 (MQ=255)

      gagggcGGTTTTTGGGGTTGCCTTAATGCCTTGGAACGGAGGAAA‑GGGGTGATTGAAGCCGGGGCAGCGGCAGTTCACTTCGAAGAGCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACGCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                                                                                                                                                       <  1:680818/233‑1 (MQ=255)

      gAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGGGGCGGGCGTTCCAAACCTCCCGTTGGCCCGTACCGAGCCCGATgcggc                                                                                                                                                                                                                                      >  2:566702/1‑239 (MQ=255)

        atgcGGGTTTGGCGGTTTTCTTATTGCCTTTGAACTTATGAAAGGTGGTG‑TTGAAGCCGGTTCAGCGGCATGTACCTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑GGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcggcg                                                                                                                                                                                                                                     <  1:326677/235‑1 (MQ=255)

            ggTTTTGGCGGTGTCCTGAATGCCTTGGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCt                                                                                                                                                                                                                                <  2:156878/239‑1 (MQ=255)

             gTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCCCTTCGAAGATCTGCTGGCGCCAGTGACGAC‑ATTCGGTCACA‑TGGGCGGCAAAGTTTTAATGCCAATCCAGGATGCTATTCAGAAAGTGGTCGCGGACCGTCCGTAAGCCG‑ACGCGTCGCACGCTTCCACCCCCCAGGTTTCCCCTACCGCCCGCGATGCGGGGGATcc                                                                                                                                                                                                                                >  1:512711/1‑237 (MQ=255)

             gTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGGGGTCCCA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTGCGTCAACTGAACGT‑ACGGGCGGTCCCACCCCGCCGGTGGCCCGGTCCGATGGTGTAGCGGGGGACCTg                                                                                                                                                                                                                               >  1:885963/1‑239 (MQ=255)

                ttGGCGGGTTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGGGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATc                                                                                                                                                                                                                            <  1:950167/239‑1 (MQ=255)

                      gtgtCCTGAAGGCCTTGGAACTGATGAAA‑GGGAGGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTc                                                                                                                                                                                                                       <  2:840583/238‑1 (MQ=255)

                        gtCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGGGCTGagaccccccgg                                                                                                                                                                                                                    >  2:459347/1‑229 (MQ=255)

                        gtCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGGCACC‑TGGGCGGCAAAGGTTTAGTGCCACATCAGGAAGCTATTCAGAAACTGGTGGCGGGGGGTCGTGCAGCTG‑GCCGGGAGGGCGGTCAAACCCCGCCGGGTTCCCCGACCGGTGGTGATGGGGGGGGTccgcagaaccccca                                                                                                                                                                                                                    >  1:978433/1‑226 (MQ=255)

                         tCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATc                                                                                                                                                                                                                            <  2:102765/230‑1 (MQ=255)

                             taaTGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGGAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                                                                                                                                                               <  2:76070/238‑1 (MQ=255)

                             gagttccTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                                                                                                                                                               <  2:784852/234‑1 (MQ=255)

                             gAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCg                                                                                                                                                                                                               <  2:1126074/239‑1 (MQ=255)

                              aaTGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGGGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGGGCGTCTGGCAGCTG‑ACGGTCAGGGGGTTCAAACCCGCCCGGTTGCCGGGACCCAGGCGGAGGcggggggagctattcacccccgtgggg                                                                                                                                                                                                               >  1:1126074/1‑217 (MQ=255)

                               atcgcTTTGAACTGGAGAAA‑GCGATGAGTGAGGCTGGTGCAGCGGCAGTTCACGTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑GGGGCGGCAAAGTTTTAGGGCCAACTCAGGAAGCTAGTCAGAAACTGGTCGCGGCGCCTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGAc                                                                                                                                                                                                             <  1:630168/235‑1 (MQ=255)

                               aTGCCTTGGAACGTATGAAA‑GCGAAGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGAc                                                                                                                                                                                                             <  2:499795/239‑1 (MQ=255)

                                   cTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGAACTGGTTACCTCCGATTCCGAccc                                                                                                                                                                                                           >  2:816381/1‑237 (MQ=255)

                                            gatgaAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCg                                                                                                                                                                                                <  2:762994/239‑1 (MQ=255)

                                             atgaAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGCTGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTAttaaacgga                                                                                                                                                                                               >  2:6499/1‑231 (MQ=255)

                                             atgaAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGTACTGATAACCCCCGGTTGCGGCCCCAATGACAGGGa                                                                                                                                                                                               >  2:512878/1‑239 (MQ=255)

                                                         gggttgagcggggtccgggggaGTTCACTGCGAAGAGCGGGTGGCGGCAGTGAAGAA‑ATGCGGTCACA‑GGGGGGGCAAAGTTTTAGTGCAAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCg                                                                                                                                                                                    <  1:711851/218‑1 (MQ=255)

                                                         gatTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCg                                                                                                                                                                                    <  2:1210676/239‑1 (MQ=255)

                                                           ttgagtcgggTGCAGTGGCAGTTGCGGTCGAAGATCGAGTGGGGTCAGTGAAGGA‑AGGCGGTCAAA‑TGGGGGGCAAAGTTTTAGTGCAAACCCAGGAAGCTATTCAGAAACTGGTCGGGGGTCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCggt                                                                                                                                                                                  <  1:912500/231‑2 (MQ=255)

                                                               ggCCGGTGCAGCGGCGGTTCACTTCGAAGATCCGGTGGCGTCAGTGAAGTA‑TGGGGGTCACA‑TGGGGGGCAAAGGTTTAGTGCCACCTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAg                                                                                                                                                                               <  1:255316/237‑1 (MQ=255)

                                                                    gTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGAGGCTGATGCGGCGGATCTGATCACCTCCGGTTGCGACCCTTTTTAACGGGGATTTATTTCCGGCggaggcacc                                                                                                                                                                         >  2:1195852/1‑231 (MQ=255)

                                                                         gCGGCAGTTCCCTTCGAAGATCAGCTGGCGTCAGGGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTg                                                                                                                                                                     <  2:441215/238‑1 (MQ=255)

                                                                              aGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                                                                                                                                                <  2:502980/238‑1 (MQ=255)

                                                                              aGTTCACTGCGAAGATCAGCTGGCCTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGc                                                                                                                                                                <  2:369416/238‑1 (MQ=255)

                                                                                  tatttggagtgttggcgggCGTGAGTGAAGAA‑ATGCGGGCAAA‑TGGGGGGCAAGGTTGTAGTGACAGTGCAGGAAACTATTCAGAAACGGGTCGCAGCGCGTGTGGAAGCTG‑ACGGAGCGGGCGTTCCAACCCTGCTGGTTGCCCGTACAGATGCTGATGCTGCGGATCTGATCACCTCCGATTGCGACCCGAATGACAGCGAATTTATTACCGGCGAGCGTCCCAGTGAAGGcttctt                                                                                                                                                           <  1:1008583/222‑1 (MQ=255)

                                                                                  cctctccaaGGTCAGGTGGCGTCCGGGAAAGA‑ATGCGG‑GACATTGGGGGGGAAAGTTTTGGGGACAAGTCAGGAAGCTATTCAGAAACTGGTCGTGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGcttctt                                                                                                                                                           <  1:1215350/232‑1 (MQ=255)

                                                                                  ccattCGAAGGTCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCCCA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGcttctt                                                                                                                                                           <  2:52526/236‑1 (MQ=255)

                                                                                    cgtCGAAGATCAGCGGGCGTCAGGGAAGAA‑AGGCGGTAACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTcc                                                                                                                                                         <  2:10329/237‑1 (MQ=255)

                                                                                     gtCGAACAGCAGCTGGGGTCAGTGAACAA‑ATGCGATCACA‑TGGGCGGCAAAGTTTTAGTGCCAACCCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCGGGCAGCTG‑ACGGTACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCg                                                                                                                                                        <  1:805669/238‑1 (MQ=255)

                                                                                      ttgagaataggCTGGGGTCATTGAAGAA‑ATGGGGTCACA‑TTGGCGGCAAAGTGTTAGTGCCAACGCAGGAGGCTATTCAGAAACTGGTCGCGGTGCGTCTGGCAGCTG‑CCGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGt                                                                                                                                                       <  1:444285/229‑1 (MQ=255)

                                                                                       agAAGATCATGTGGCG‑CAGGGAAAAAGATGCGGTAAAA‑TCGGCGGAAAGGTGTTAGGGCCAAGTCAGGAAGCTATTCAGAAACGGGGCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTa                                                                                                                                                      <  1:809492/238‑1 (MQ=255)

                                                                                             tttgccggctgtaGTGAAGAA‑TTGCGGTCCCA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATg                                                                                                                                                <  1:276225/227‑1 (MQ=255)

                                                                                              ccgCTGGCGCCAGGGAAAAA‑AGTCGGTCACA‑TGGGGGGCAAAGTTTTAGGGCCAACCCAGGAAGCTTTTCAGAAACTGGTCGCGGGGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATg                                                                                                                                                <  1:1307943/236‑1 (MQ=255)

                                                                                               aGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGGGCGTACCAGGGAAGGCCTCTTCCGTACTCAAGCg                                                                                                                                              >  2:1307943/1‑239 (MQ=255)

                                                                                                  tGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGc                                                                                                                                               <  2:978850/235‑1 (MQ=255)

                                                                                                         tgTGGAGAA‑ATGCGGTCCCA‑TGGGGGCGAAATTTTTAGTGGCAACTCAGGAAGCTATTCAAAAACTCGTCCCGGCGCGTGTGGCAGGTT‑ACGTGGCGGGCGTTCCAACCCGGCTGGTTGCCCGTACCGAGGCTGATGCGGCGGATCTGATCACCGCCGATTGGGACCCGTATGACAGCGAATTTATTACCGGCGTGCGTTCAAGTGAAGGCTGTTCCCGTATCCATGCGTCCATTGAGc                                                                                                                                    <  1:988786/238‑1 (MQ=255)

                                                                                                         aGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTGAGGAAACTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAg                                                                                                                                     <  2:1317419/238‑1 (MQ=255)

                                                                                                          gTGAAAAA‑AGGCGGTAACA‑AGGGCGTGAAGGTTTTAGGTCCAACCCAGGAAAGTCGTGAGAACCTGGGCGCGGCCGCTCTGGCACCGG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCCGATCTGATCACCTCCGAGTGGGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGc                                                                                                                                    <  1:560082/238‑1 (MQ=255)

                                                                                                           tGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTg                                                                                                                                                                     <  2:189562/204‑1 (MQ=255)

                                                                                                            gaagaa‑ATGGGGTCACA‑GGGGGGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCaa                                                                                                                                  <  2:530969/238‑1 (MQ=255)

                                                                                                                aa‑aTGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGAt                                                                                                                             <  2:478693/239‑1 (MQ=255)

                                                                                                                 a‑aTGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGAt                                                                                                                             <  2:1309350/238‑1 (MQ=255)

                                                                                                                      cGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATTGGGGCATTGGGCAAGGGATGAGcc                                                                                                                        >  2:772158/1‑239 (MQ=255)

                                                                                                                         gtgccgtGGGGGGAAAGGTTTTGGTGCCAACTCAGGAAGTTTTTCTGACACGGGGCGGGGTGCGTTGGGCGGCTG‑ACGTGCCGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGt                                                                                                                      <  1:393382/233‑1 (MQ=255)

                                                                                                                                      cacaGTTTTAGGGCCAACCCAGGAAGCTTTTCGGAAATGGGCCGCGGCGCGTCTGGCAGGTG‑ACGTGACGGGCGTTCCAACCCGGCTGTGTGCCCGTACCGATGCTGATGCGGCGGATCTGATCCCCTCCGATTGCGCCCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt                                                                                                          <  1:1031284/235‑1 (MQ=255)

                                                                                                                                      cAAATTTTTAGTGCCAACGCAGGACGCTCTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGG‑ACGTGCCGGGCGTTCCAACCCTGCGGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt                                                                                                          <  1:1053162/238‑1 (MQ=255)

                                                                                                                                      cAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGCTCTGATCACCTCCGATTGCGGCCCGTATGACAGCGAATTTATTACCGGCGATCGTACCAGGGAAGGCTTCTTCCGTACTCATGCGGGCCTTGTGCAAGCGATCAGCCGTGGCCTGGCGTAt                                                                                                          >  2:129712/1‑238 (MQ=255)

                                                                                                                                      cAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt                                                                                                          <  2:1069010/238‑1 (MQ=255)

                                                                                                                                      cAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACGGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt                                                                                                          <  2:11173/238‑1 (MQ=255)

                                                                                                                                      cAAAGTTTTAGTGCCAACCTAGGAATCTATTCAGAAACTGGTCGCGGCGGTTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTAt                                                                                                          <  2:1266722/238‑1 (MQ=255)

                                                                                                                                       aagtttttAGTGCCAACTCAGGAAGCTAGTCAGAAACTGGTCGCGGCGCGTCTGGCAGCGT‑ACGGGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATg                                                                                                         <  2:1024675/234‑1 (MQ=255)

                                                                                                                                            tttAGTGTCAACTCAGGAAGCTAATCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc                                                                                                     <  2:990536/237‑1 (MQ=255)

                                                                                                                                            tttAGTGCCCACCCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc                                                                                                     <  2:703534/237‑1 (MQ=255)

                                                                                                                                            tttAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc                                                                                                     <  2:901437/237‑1 (MQ=255)

                                                                                                                                            tttAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGcc                                                                                                     <  2:1136103/237‑1 (MQ=255)

                                                                                                                                               ttggcgccctcgggtattgtttCAGACACGTGCCGCGGCGAGCTCGGCAGGTG‑ACGTGCGGGGCGTTCCACCCCTTCTGGTTGCTCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCTATTGCGACCCGTATGGCAGCGACTCTATTCCCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATg                                                                                                <  1:112830/219‑1 (MQ=255)

                                                                                                                                               aggcacAACTCAGGGAGCTATTCGGAACCTGGGCGCGGCGCGTCTGGCAGCTG‑ACGTAACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGAGGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATg                                                                                                <  2:710537/234‑1 (MQ=255)

                                                                                                                                               aGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCCCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCGCCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATg                                                                                                <  2:469256/239‑1 (MQ=255)

                                                                                                                                                               gCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                                                                                                                                                       <  1:1140650/88‑1 (MQ=255)

                                                                                                                                                               gCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATgcgg                                                                                                                                                                                                                                       >  2:1140650/1‑88 (MQ=255)

                                                                                                                                                                 tATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCa                                                                                                                                          <  2:455646/179‑1 (MQ=255)

                                                                                                                                                                  aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGGGCAAGCGATAACCCGGTGCCTGGCGTATGCGCCCTAAGCTGACCCGGTCTGGTGTGa                                                                             >  2:294127/1‑239 (MQ=255)

                                                                                                                                                                         aaCTGGGCGGGGGGCGGCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTcc                                                                      <  2:1250058/239‑1 (MQ=255)

                                                                                                                                                                             ggTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACCGCGAATTTATTACCGGCGAGCGGACCAGTGAAGGCTTCTTCCCGACTCATGCGGGGCTTGGACAAGCGATACTCGGTGGCCTGGGCGATGCGCCATACGCTGACCCGGTCTGGGGTACAAACCCCACGc                                                                  >  1:259817/1‑239 (MQ=255)

                                                                                                                                                                             ggTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCCTTCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATCGGCCCCATCCTGGCCTGGGCTGGGGTGAAACccccccc                                                                   >  2:47872/1‑235 (MQ=255)

                                                                                                                                                                             ggTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGGGTGAAACCTCCACg                                                                   >  2:967784/1‑238 (MQ=255)

                                                                                                                                                                             ggTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCATGGCCTGGCGTATGCGCCATATg                                                                                                >  2:400002/1‑209 (MQ=255)

                                                                                                                                                                                                      cctcacgGGCGTTCCTACTCTTCTTTTTGCCCTTTCCGTGGGTGAGGGGGCGGATTTGATCCCCCCCGATTGCGACCCGTATGACAGCGAATTTATTTCCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCt                                          <  1:459347/235‑1 (MQ=255)

                                                                                                                                                                                                            gggCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑ca                                    <  2:831870/238‑1 (MQ=255)

                                                                                                                                                                                                                gTTCCCACCCTGCTGGTTGCCCGTACCGAGGCGGATGCGGCGGTTCTGATCCCTCCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAg                                <  1:129712/238‑1 (MQ=255)

                                                                                                                                                                                                                gTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAg                                <  2:202418/238‑1 (MQ=255)

                                                                                                                                                                                                                gTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAg                                <  2:103123/238‑1 (MQ=255)

                                                                                                                                                                                                                      aCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCa                         <  2:1266690/239‑1 (MQ=255)

                                                                                                                                                                                                                      aCCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCa                         <  2:149195/239‑1 (MQ=255)

                                                                                                                                                                                                                      aCCCTGCTGGTGGCCCGTACCGATGCGGATGCGGCGGATCTGATCCCCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCa                         <  2:43851/239‑1 (MQ=255)

                                                                                                                                                                                                                           gctgGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACCGCGAATTTATTACCGGCGAGCGTACCAAGGAAGGCTTCTTACGTACTCATGCGGGCATTGAGCAAACGATCATCCGCGGCCTTGCGTATGCGCCATATTGTGAACTGGTCTGGGGTGAAACCCCCACGCCGGATCAGGAAATGGCgcggcggcttgttaaagaacacaccccgc                     >  1:54843/1‑216 (MQ=255)

                                                                                                                                                                                                                           gctgGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGGATGCGCCATATGCTGACCTGGTCTGGTGGGAAACCTCCACGCCGGATCTGGAACTTGCGCGTCGCTTTG‑CTCAAGCTTTCCCCCCGa                    >  2:76997/1‑239 (MQ=255)

                                                                                                                                                                                                                            ctgGTTGCCCGTACCGATGCTGATGCGGCGGTTCTGATCACCTCCGATTGCGACCCCTATGATAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTGCAAATTCTGGCATTGTGCAAGCGATCAGCCGAGGCCTGTGGGATGCGCCCTATGCTGCACCGGGCGGGTGGGAAACCCGCCCCCCCGGTTTGCAACTGGCGGGGCGGTGTGACAAAACCTACCCAcgcag                    >  1:749300/1‑237 (MQ=255)

                                                                                                                                                                                                                            ctgGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCCCGTCCCCTTG‑GCCAAACTATCCACGCCaa                   >  2:982149/1‑239 (MQ=255)

                                                                                                                                                                                                                            ctgGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGGCTGGTGTGAATCCTCCACGCCGGATCTGGAACTGGGGCGTCGCTTTG‑CCCAAGCGATcccccggac                   >  2:343713/1‑232 (MQ=255)

                                                                                                                                                                                                                            ctgGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGCGAAGGCTTCTTCCGTACTCATTCGGGCATTGAGCAAGCGATCAGCCCTGGCCCGGCCGATGCCCCATATTGTGACCTGGGTTGGTGGGAAACCTCCACCCCCGGACCGGAAACGGCGCGTGCCTTTggcaaaacaaacccaccgaa                    >  1:247011/1‑220 (MQ=255)

                                                                                                                                                                                                                                 tGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACCCGAAatat               >  2:20673/1‑238 (MQ=255)

                                                                                                                                                                                                                                  gCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGACCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAACATcc             >  2:1061758/1‑239 (MQ=255)

                                                                                                                                                                                                                                  gCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGGGAAACCTCCACGCCGGATCTGGGACTGGCGCGTCCCTTTG‑CCCACACTATCCACGCGAAACATcc             >  2:1223165/1‑239 (MQ=255)

                                                                                                                                                                                                                                       tACCGATGGTGAGGGGGCGGATCGGATCCCCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAATATCCGGGCa        <  2:684051/239‑1 (MQ=255)

                                                                                                                                                                                                                                        tccGATGCTGAAGCGGCGGATCTGATCCCCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAATATCCGGGCa        <  2:698544/237‑1 (MQ=255)

                                                                                                                                                                                                                                             tGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGc                                                                                                                                                                                                 <  1:881188/49‑1 (MQ=255)

                                                                                                                                                                                                                                             tGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGc                                                                                                                                                                                                 >  2:881188/1‑49 (MQ=255)

                                                                                                                                                                                                                                              gCTGGTGCGGCGGATAGGATCCCCCCCGTTTGCGGCCCGTATTACCGCGAATTTATTACCGGCGAGGTTACCAGGGAAGGCTTCTTCCGTACTCAGGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAATATCCGGGCAAActgc  <  1:76997/238‑1 (MQ=255)

                                                                                                                                                                                                                                              gCTGATGCTGCGTATCTGATCCCCACCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAATATCCGGGCAAActgc  <  2:87680/238‑1 (MQ=255)

                                                                                                                                                                                                                                              gCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAATATCCGGGCAAActgc  <  2:924061/238‑1 (MQ=255)

GATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAA‑GCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAA‑ATGCGGTCACA‑TGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG‑ACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTG‑CACAAGCTATCCACGCGAAATATCCGGGCAAACTGC  >  NC_000913/4217576‑4218047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`