Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 433,649 T→G 39.7% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913433,6490TG39.7% 26.1 / 11.2 53G65G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (10/20);  new base G (16/6);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

TTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGC  >  NC_000913/433419‑433883
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGAGGCGAccc                                                                                                                                                                                                                                         >  2:193026/1‑237 (MQ=255)
 ttggcagaggtcccggccgcTTGGGGGACAAGCCCGTGGAGGGCGTGTATTAAGGGGCGGGGGACTAAAAGTTGGCGCAAGGGGTCCGTTTTCCCACGCCTCCCACCCCGAAGGGCGGGTGCGTAATTGTAAAAGAGGCAAAATTTGTGGGTAAGGGTTCCCACAAAGTGGGGGGTAAACCACAGGCCGAAGTACACGCGTGGCGTATGGGGGGTGAAAAAGCCAAAGGTGCGACCGcc                                                                                                                                                                                                                                      <  1:1264585/222‑1 (MQ=255)
              cgcgcgCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTTTGGCGGGGGGGAAAACCAAAAGGGGGACCCCCCATGTccaacccg                                                                                                                                                                                                                        >  2:882922/1‑232 (MQ=255)
                    ccTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCCAAAGGGCGACCCCCCATGTCACCCTCGAAccc                                                                                                                                                                                                                   >  2:67511/1‑238 (MQ=255)
                        gaggaCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGGCAAAGGGGCGACcccccactccaacatccaaccccgta                                                                                                                                                                                                                >  2:1305509/1‑216 (MQ=255)
                             cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCCCTGTCAACCTCGAACCCTCTAGCcatc                                                                                                                                                                                                         >  2:41358/1‑239 (MQ=255)
                              gatgCCGTGCGGGAGTAGTATGACATGGCGCGGGCTCAAAAGGGTGCCCACGGTGTACTTTGTACCCCCCATCCAACCACGAAGGTGGGTGGCTTCATTGTAAAAGAGGGCAAATTTGCCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATCCCGAAGTCCACCCTTTGCGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatca                                                                                                                                                                                                        <  1:41358/236‑1 (MQ=255)
                                aGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCCAAAGGGCGACCGCCCATGTCACACTCCAACCCCGTAGCCATCATg                                                                                                                                                                                                      >  2:214080/1‑239 (MQ=255)
                                    gTGCAGGACGAGTTTTACAGGGCGGGGGCGATAAAGCGGGCGCAACGAGGACGTTTTCCCACGCATCCCACCCCGAATGTCGGGTGCTTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCg                                                                                                                                                                                                  <  2:1177589/239‑1 (MQ=255)
                                     tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAAGGGCGACCGCCTATGTCACACTCGAACCCCGTAGCCCCCATcgtcgt                                                                                                                                                                                                 >  2:1284265/1‑239 (MQ=255)
                                     tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAAGGGCGACCGCCCATGGTCACCTCGCAACCCGTAGGCCACATGGTCGt                                                                                                                                                                                                 >  2:1199913/1‑239 (MQ=255)
                                               gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGt                                                                                                                                                                                       >  2:190422/1‑239 (MQ=255)
                                               gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAGGGGGGGCCCCCTCTTTCACACCCGCACCCCGGTGCCACCCTGGGCGTAcccccc                                                                                                                                                                                          >  2:576169/1‑234 (MQ=255)
                                                tATCACATGGCGCGGGCGCTAAAGCTGGCGCAAGGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                              <  2:599955/199‑1 (MQ=255)
                                                     cgtgggcggggCGCTAAAGGTGGCGCAACGAGGACTTTTTCCCGCGCATCCCAACCCGAATGGCGGGTGCGTAATTGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgtg                                                                                                                                                                                 <  2:863996/231‑1 (MQ=255)
                                                        ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTTTTTCACACTCGAACCCCGTAGCCACCATGGGCTTACGCCACCCGCCTGTGAc                                                                                                                                                                               >  2:924888/1‑238 (MQ=255)
                                                          cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGAACGCCTATGTCACACTCGAACCCTTTAGCCAACATGGGCGTACCGCACCGGGcctgtgcgc                                                                                                                                                                             >  2:726619/1‑230 (MQ=255)
                                                            cgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCCCTGTCCCACTCGAACCCTGTAGCACTCATGGTCGTACGCcccccgtctcttttaccc                                                                                                                                                                           >  2:761881/1‑224 (MQ=255)
                                                                           gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGAACGCCCAGTTACCACCCCAACCCCGTAGCCATCAACGCCCTACGCCACCGTGCTGTGGCGCACCCATACCCGCCGGCgc                                                                                                                                                           >  2:775452/1‑238 (MQ=255)
                                                                                 cGAGGGCTTTTTCCCCCGCACCCCAACCCGAATGGGGGGGGCGTCATTGAAAAAGATGGCGAAATTGGCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcgcg                                                                                                                                                     <  2:253464/239‑1 (MQ=255)
                                                                                        tttttACCACGCCTCCCAACCCGAATGTGGGGTGCGTCATTGTCAAAGATGGCGAAATGGTCGGTAAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGGGTTGCGTAGGGCGGGTAAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGt                                                                                                                                               <  2:1286785/237‑1 (MQ=255)
                                                                                                 cGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGGCACCGCCTATGGCACACTctga                                                                                                                                                                                                                      >  2:904652/1‑155 (MQ=255)
                                                                                                   cATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCCCTGGCGTAGCGCGCGTGGGTGCCTCGAGGCa                                                                                                                                  >  2:868874/1‑239 (MQ=255)
                                                                                                                aTGGCGGGTGCGTCATTGTCAAAGGTGGCGAAATTGTCGGTAAAGGTTACCACCAACGTGGGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGCGGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCg                                                                                                                     <  2:449975/239‑1 (MQ=255)
                                                                                                                      ggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCCCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGt                                                                                                                >  2:1154930/1‑238 (MQ=255)
                                                                                                                          cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCAAAGGGCGGCCCCCCCTGTGCCCCTCGAAACCCGTAGCCATCATGGTCGTACGCCCCCCCGCTGTGGCGCCCCCCACCCCGCCGGCCTAGCGCCCGGGGGTGCCCCGCTGCCAAAACCCAACCCACAgggggggg                                                                                                           >  1:449975/1‑233 (MQ=255)
                                                                                                                             cATGGTAAAAGGTGGCAAATTTGCAGGTGACGGTTACCCCAAACGTGGGGGTGAACCCCAGGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                                        <  2:155321/239‑1 (MQ=255)
                                                                                                                               tgtcaaaaGATGGCGAAATTGTGGGGTAAGGTTACCCCCAACGGGGGGGTAAACCACAGGCCGAAGTACACGCGTGGCGTATGGGGGGTAAAAAAGCAAAAGGTGGGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGt                                                                                                      <  2:386828/234‑1 (MQ=255)
                                                                                                                                         gggCAAAATTGGCGGTGAAGGTTACCCCAAGCGTGGGGGTAAACCCAATGCCGAAGTCCCGCGGTTGCGTAGGGCGGGTTAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTa                                                                                              <  2:400068/236‑1 (MQ=255)
                                                                                                                                             cgattttttCGGTAGAGGTTCCCACATACGTGCGGGTAAACCACAGGCCGAGGTACCCGCTTGGGGTAGGGGGGTAGAAAAGCCCAAGGGTCGGACCGCCTATGTCACCCTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGt                                                                                          <  1:214080/230‑1 (MQ=255)
                                                                                                                                                tttggtgggTGATGGTTCCCACCCGCGGGCGTGCAAACCAGATGCCGACGTACCCGTGTTGGGGTTGGTGGGTAAAAGACCCAAGGGTGCGACCGCCTATGTCCCCCTCGAACCCTGTTGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTg                                                                                       <  1:332363/231‑1 (MQ=255)
                                                                                                                                                                 accaccAACGTGCGGGTGAACCACATGCCGAGGTACACGCGTTGCGTATGGCGGGTGGCAAAGCCAAAAGGGCGACCGCCCATGTCACCCACGAACCCCGGAACCATCATGGTGGTACGCCCCCGCGCCCTTACGCACTCCTCTCCCCAGGCGGAGCGACCCAGGTTGCCCCCGAGACAAGTCCTAACCCCCAGGGCGCCTGGCGGGGGCTCTAccctactccaaaagccggggttgcg                                                                     >  1:277060/1‑216 (MQ=255)
                                                                                                                                                                       aaCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTTTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACCCATCGCCGCTGGCGTAGCGCGCGGGGTTGCCTCGGTGCAAGATCCTAACCCCCCGGTCGCCGGGCGTGGACCTTTCCGGCTGCCACAGGCTGGCATTGACGTCAg                                                               >  2:947268/1‑238 (MQ=255)
                                                                                                                                                                         cgggCGGGTGACCCATGTGCCGAAGTCCCCGCGTTGCGTATGGCGGTGAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACGCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                            <  2:1251738/236‑1 (MQ=255)
                                                                                                                                                                         cgggCGGGTAAACCCCATGCCGAAGTCCCCGCGTGGCGTATGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                            <  2:34168/236‑1 (MQ=255)
                                                                                                                                                                           tGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGGGCGGGCTTTTCCGCCTGCAACAGGCTGGCATTGACGCCGGCCCCg                                                          >  2:369194/1‑239 (MQ=255)
                                                                                                                                                                            gggggAGAACCCCATGCCGAATGACCGTGTTGGGGTGGGGGGGGGAAAAAACCAAAGGGTGGGGCCGCCTTTGGCACACTCGAACCCTGTGGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg                                                          <  1:486136/236‑1 (MQ=255)
                                                                                                                                                                             cGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGGGCGACCGCCCATGTCACACTCGAACCCCGTAACCATCCTTGTCCTACGCCACCCGGCTTTGAGGCACGCACTGCCGCCGGCGCATCGCCCCGGGGTGGCTCGTTGGAGAACACCAACCCGCCGGCGCCTGGGCCGGGCCTTTACCGGCTGAAAACGGCTGGccatgcaagcagacacgcc                                                         >  1:14619/1‑221 (MQ=255)
                                                                                                                                                                              gggtaaacccattGCCAAAGTACACGGGTTGGTTAGGGGGGGTAAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                                        <  2:250050/226‑1 (MQ=255)
                                                                                                                                                                                     cccccggcccggatgtcccggtgtGCATAGGGGGGGTGAAACGACAAAGGGGCGGCCTCCCTATGGCCCACCCGAACCCTGTAGCCATCGTGGACGTCCGCCACCGGGCTGTGACGCTCGTTTGGCCGCTGTCGAAGCGCGCGTGGTTGCCTCGATGCAAGATTCTATCCCGCAGGTCGCTGGGCGTGGACTGTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                  <  1:1279906/215‑1 (MQ=255)
                                                                                                                                                                                             gcagaagctcccgttggCGTTTGGGGGGAGAAAACCCCAAAGGGGGCGCCCGCTTAGTTCCCCCTCGAACCCTGTGGCCACCATGGCCGTACGCCCCCGGGCGGTGACGCACTAATCGCCGCGGGCGTAGCGGGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAg                                             <  1:880179/220‑1 (MQ=255)
                                                                                                                                                                                               aaGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCCGGTCGCTGGGCGGGGACTTTACCGTCTGCCACAGGCTGGCATTGACGTCAGCCCCGGCCCGATGGTGAGGGAAGcc                                      >  2:915124/1‑239 (MQ=255)
                                                                                                                                                                                                attccccgcgTTGGGTAGGGCGGGTGAAAAACCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                                     <  2:1295641/233‑1 (MQ=255)
                                                                                                                                                                                                   tactgcggttggtgtgggCGGGGAAAAAGCCAAAGGGGGCACCCGCCTTTGTCCCACTCGAACCCCGTAGCCATCAGGGGCGGACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTCGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                   <  1:576169/223‑1 (MQ=255)
                                                                                                                                                                                                    acccgggTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                   <  2:277060/232‑1 (MQ=255)
                                                                                                                                                                                                           ttGCGTATGGCGGGTGGAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGGACCCTGTAGCCATCATGGTCGGACGCCCCCGGGCTGTGACGCACTCCTCGCCGCTGGCGGAGCGCGCCTGGGTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGTGGACTTTACCGTCCGCAACAGGCCGGCATTTGCGTCCGCCACCGCCTGATGTTAGGGGAAACCGGGCAATTGaa                           >  1:229860/1‑239 (MQ=255)
                                                                                                                                                                                                             gCGTATGGGGGGGAAAAACGCAAAGGGGGCGACCGCCTATGTCACCCTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATa                         <  2:14619/239‑1 (MQ=255)
                                                                                                                                                                                                              cGTAGGGCGGGTGAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGt                                                                                                                                                                                                    <  2:58994/69‑1 (MQ=255)
                                                                                                                                                                                                                 gtGGGGGGTGAAAAGCCAAAAGGTGTGCCCGCCTATGGCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAg                      <  2:404270/237‑1 (MQ=255)
                                                                                                                                                                                                                  gggCGGGAAAAAAAGCCAAGGGGGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGc                    <  2:687243/238‑1 (MQ=255)
                                                                                                                                                                                                                        ggggAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCCTGCTTTGCAGCCCTCATCGCCCCTGGCGTAGCGCGCCGGGTGGCCTCGATGCAAGATCCTAACCCGCAGGGCGCCGGGCGTGGCCTTTACCGTCTGCAACAGCCTGGCACTTCGCGCCGCCCCGGCCCCAAGATGAGTGAAACCGCGAAAATGaaaaaaagggtttc               >  1:687243/4‑231 (MQ=255)
                                                                                                                                                                                                                                      aGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGCCTTTCTCAAGCGGATgggc  >  2:534743/1‑238 (MQ=255)
                                                                                                                                                                                                                                      aGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGGGAGGCCGACCAATTGAATAAAGGCTTTCTCAAGCGGCTgcgc  >  2:204363/1‑238 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
TTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGC  >  NC_000913/433419‑433883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: