Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,313,537 A→G 9.7% I63V (ATT→GTT)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,313,5370AG 9.7% 285.7 / 2.1 115I63V (ATT→GTT) rpiBribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (43/53);  new base G (13/2);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 4.14e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTA  >  NC_000913/4313301‑4313774
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  
cATCTCAAATGGTTGTTTGTTGTGAGGTTTTTGACTGACGGGGAAGATGAATGAAAAAGATTTCTGTTGTCTGTTATCAGGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCTCATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGAtt                                                                                                                                                                                                                                                  <  1:821035/239‑1 (MQ=255)
   cacaAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACTTTACGCCAGTCAAGTCGCACTGGCGGTGTTTGGCGGTGAGGTGGATGGGGGGGTTTTg                                                                                                                                                                                                                                               >  1:971158/1‑239 (MQ=255)
     caAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCCCATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGACGTCAGAGCGTACTGATTATCCCCATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAAAGGTTGATGGGGGGGTTTTggt                                                                                                                                                                                                                                             >  2:657763/1‑237 (MQ=255)
     aaaaTGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGAt                                                                                                                                                                                                                                             <  2:38928/238‑1 (MQ=255)
           ttttttGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGGttttggg                                                                                                                                                                                                                                       >  2:641652/1‑235 (MQ=255)
             ttttGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTGAGCCAGTCAAGGCGGCCTGGCTGTTGCTGGGGGTGGGGGTGAGGGGGGGGGTTTGATTTTTG‑Gtg                                                                                                                                                                                                                                    >  1:177022/1‑238 (MQ=255)
                tGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGATTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGAGCGGCGTTGAAGTGATTGATGAAGGAACCTGGTCGGCAAAGCGTACTGATTATCCACATTAAGCCAGTCAAGTCGCACAGGCTGTTGCTGGCGGAGAGGTTGAGTGCGGGATGTTGTTTTGTG‑GTAATg                                                                                                                                                                                                                                 >  2:168979/1‑239 (MQ=255)
                     ttgAATTTTTTCACGGACGGGGAAGATGAATGAAAAAGATTTCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTc                                                                                                                                                                                                                            <  1:1043930/238‑1 (MQ=255)
                      tgAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAAAGGTTGATGGCGGGATTTTGATTTTGT‑GTACTGGCGTCg                                                                                                                                                                                                                           >  2:971621/1‑239 (MQ=255)
                             ttGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGGAtttt                                                                                                                                                                                                                    >  2:528936/1‑238 (MQ=255)
                                     acggGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTTATGGCGGGGTTTTGATTTTTT‑GTACTGGCGTCGGGATTTCGAGAac‑gg                                                                                                                                                                                                           >  2:1207037/1‑239 (MQ=255)
                                                   tgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGGGAGGGTGATGGCGGGGGTTTGATTTTTG‑GGACTTGCGGCGGGAGTTTGATAAG‑GGGGAACAAGGTTGcc                                                                                                                                                                                             >  1:920348/1‑239 (MQ=255)
                                                   tgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGAAAAC‑GGCGAAACAGTTTTcc                                                                                                                                                                                             >  2:409290/1‑239 (MQ=255)
                                                   tgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTAAGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGTGGGGGTGATGGGGGGGATTTGGTTTTTG‑GGATGGGGGACGGGGTTTTGGTAGC‑GGCGAACAAGTTTggc                                                                                                                                                                                             >  1:517960/1‑237 (MQ=255)
                                                           aTTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGTTTTTGATTTGGG‑GTACTGGCGTCGGAATTTCGATACG‑GGCGAAACAGTTTGCCGGAATTc                                                                                                                                                                                      >  2:956046/1‑238 (MQ=255)
                                                             tttatttggttttgtttttGTCGGTTTCATTTTAAAACATGAAATAGGGGCCTATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCGGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGGTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTcgcg                                                                                                                                                                                   <  1:400564/221‑1 (MQ=255)
                                                             ttcttttgggtttttttaTGTGGGTTTCATTTTAAAGCATGAAATAGTGGCACATTTAGTTGGGCGGTGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTcgcg                                                                                                                                                                                   <  1:1309568/222‑1 (MQ=255)
                                                             tccttttGGTTGTGTTGATGTGGGTTTTATTTTAAACAAAGAAACATTTGCATAGTAGGTTGAGTGTGGCGGTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTAAGGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑TGCGATCAAGTTTGCCGGAATTcgcg                                                                                                                                                                                   <  1:1049766/235‑1 (MQ=255)
                                                             tGCATTTGGCTGTGATCATGTCGGTTTCATTGTAAAACATGAAATAGTGGGACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTcgcg                                                                                                                                                                                   <  2:523659/239‑1 (MQ=255)
                                                             tGCATTTGGCTGTGATCATGCCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGCTGCGGGAGGCGTTGACGTGATTGATAAAGGAACCTGGTCGTAAGAGCGTAACGGTTATCCACATTACTCCCGTCAAGTCGCGCTGGCCGTTGCTGGCGGCCAGGCTAATGGCGGGCCTTTGTCTTTTG‑GGACTGGAGGCGGTACTTTGAGAGG‑AGGGGAGAAGTTTGCCCGaaatgccg                                                                                                                                                                                   >  1:322142/1‑233 (MQ=255)
                                                             tGCATTTGGCTGGTATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTcgcg                                                                                                                                                                                   <  2:349443/239‑1 (MQ=255)
                                                               cATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCCGGCGGAAAGGTTTATGGGGGGGATTTGTTTTTGG‑GTTCTGGGGTCGGGATTTCGATAGCGGGGGAACAAGTTTCCGGGaaatggggg                                                                                                                                                                                  >  1:1108947/1‑232 (MQ=255)
                                                               aaTTTGGGTGTGTTGATGCTTGTTTCATTTTAAAACAGGAAATAGGGGCTATTTTAGTTGAGGGTGGGGTGGAAGTGGTTGATGAAGGAACCGTGTCGACAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTcgcgcg                                                                                                                                                                                 <  1:798501/238‑1 (MQ=255)
                                                                gtttGGCTGGGATCATGTCGGTTTCATTTTAAAACAGGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTcgcgcg                                                                                                                                                                                 <  2:538698/237‑1 (MQ=255)
                                                                    ggCTGTGATCATGTCGGTTTCATTTTAAAACAGGAAATAGGGGCACATTTAGTTGGGCGTGGCGTTGAAGTTATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGgtcgt                                                                                                                                                                            <  2:1256640/239‑1 (MQ=255)
                                                                       ttttaTCATGGCGGTTTTATTTTAAAAAATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCAATGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCt                                                                                                                                                                          <  1:1099683/234‑1 (MQ=255)
                                                                         tgATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGGATTTCGATAGC‑GGCGAACAAGTTTGCGGGAATTCGCGGGGTCTCCTGTa                                                                                                                                                                       >  2:825096/1‑239 (MQ=255)
                                                                           tttattttggTTTCATTTTAAAAGATGAAATGGTGGCACATTTAGTTGGGCGGGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTAGCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGc                                                                                                                                                                     <  1:97645/231‑1 (MQ=255)
                                                                           ttgagttCGGTTTTATTTTAAAACCTGAAAGAGTGGCACATTTGGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCAGTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGc                                                                                                                                                                     <  1:667733/233‑1 (MQ=255)
                                                                           ttctttttgttttCATTTTAAAACATGAAATAGTGGCATAGTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGc                                                                                                                                                                     <  1:323231/229‑1 (MQ=255)
                                                                            tCATGTCGGTTTCATTTTAAAACATTAAATAGTGGCACATTTAGTTGAGGCTGGCTTTGAAGTGATTGATAAAGGATCCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGc                                                                                                                                                                     <  2:723591/238‑1 (MQ=255)
                                                                               tGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTTCCGGAATTCGCGCGTGCGGCTTTAGCGaa                                                                                                                                                                  >  2:425420/1‑238 (MQ=255)
                                                                                gTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGTTAGC‑GGCGACCAAGTTTTCCGGAATTTCCGCGGGCGGCTGTAGGGAAc                                                                                                                                                                 >  2:1099683/1‑238 (MQ=255)
                                                                                   ggTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGGTTTTTATTTGTG‑GGACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTa                                                                                                                                                             >  2:826222/1‑239 (MQ=255)
                                                                                        cATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GGACTGGGGTCGGGATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTc‑c                                                                                                                                                        >  2:453700/1‑238 (MQ=255)
                                                                                                 actttaaaTAGTGGCATATTTAGTTGGGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACttt                                                                                                                                              <  1:207658/234‑1 (MQ=255)
                                                                                                     gAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGGATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGGAGCGAACCTTATTC‑CGCGCAACTTTctcg                                                                                                                                          >  2:172718/1‑239 (MQ=255)
                                                                                                     gAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGCATTTCGATAGC‑GGGGAACAAGTTTGCCGGAATTTGCGCGTTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTcgcg                                                                                                                                          >  2:622108/1‑239 (MQ=255)
                                                                                                      aaaTGGTGGCAATTTTAGTTTGGGGTGGCTTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGACAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCgg                                                                                                                                         <  1:485875/239‑1 (MQ=255)
                                                                                                      aaaTAGTGGCACATTTAGTTGGGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCgg                                                                                                                                         <  2:310718/239‑1 (MQ=255)
                                                                                                       aaTAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCgg                                                                                                                                         <  2:162836/238‑1 (MQ=255)
                                                                                                          aggtggccttttATTTGAGGGTGGCGTTTAAGTGTTTGATAAAGGGACCTGGTCGTCAGAGCGTACTGGTTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCGGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGgcagc                                                                                                                                     <  1:1228450/230‑1 (MQ=255)
                                                                                                              gCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGCTTTTGGTTTGTG‑TTACTGGCGTCGGGATTTTGAAAAG‑GGCGAAAAAGGTTGCCGGGATTTGCGCGGGTGTCTGGTGCGAACCTTCTTc‑ccccccatttttcggcgccacaaa                                                                                                                                 >  1:1253076/1‑216 (MQ=255)
                                                                                                                      aGTTGGGCGTGGCGTTGAAGTGATTTATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCa                                                                                                                         <  2:660253/239‑1 (MQ=255)
                                                                                                                            gCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGGTTTTGATTTTTG‑GTACTGGCGTCGGGATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTTTGTAGCGAACCTTATTC‑CGCGCAACCTTCGCGGCAGCCTAACGAAACCAACgggt                                                                                                                   >  1:958548/1‑236 (MQ=255)
                                                                                                                             cGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCCTAACGaccgtccctttta                                                                                                                  >  2:814568/1‑228 (MQ=255)
                                                                                                                             cGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCCTAACGAAACCAACgg                                                                                                                     >  2:1238815/1‑235 (MQ=255)
                                                                                                                             cGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGGTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGGATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCCGGGTCGTCCGTAGCGAACCTTATTC‑CCGCCAACTTTCCCGGCAGCGTAAAGACACCAACGt                                                                                                                     >  2:461985/1‑236 (MQ=255)
                                                                                                                              gTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCt                                                                                                                  <  2:922213/238‑1 (MQ=255)
                                                                                                                              gTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CCCGCAACTTTCGCGGCCGCATAACGACCCCAACATGCTg                                                                                                                 >  2:790889/1‑239 (MQ=255)
                                                                                                                                    ttgaaggttttgagAAAGGAGCCTGGTCGGCGGAGGGTACTGATTTTCCACATTACGGCAGTATAGTTGGTCGTGCTGTTGCTTGCGTAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCttt                                                                                                            <  1:1194560/229‑1 (MQ=255)
                                                                                                                                      gAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTgg                                                                                                         <  2:1057191/239‑1 (MQ=255)
                                                                                                                                            attgatAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGGTTTTGATTTGTG‑GTACTGGCGGCGGGATTTCGGTAGC‑GGGGAACAAGGTTTCCGGAATTCGGGCGGGCGGTTGTGCAGAACCTTATTC‑CCCCCCAATTTCCCGGCACCATAAAGGCACCCAAGCGGCGGCCTTTGG‑TTCACg                                                                                                  >  1:589712/1‑239 (MQ=255)
                                                                                                                                            attgatAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGGTGATGGCGGGGTTTTGATTTTTG‑GTACTGGCGTCGGTATTTGGGATGC‑GGGGAGAAAGTTTTCCGGAATTCTGCCGGTGGGTCGTATGGAACCTTTTTCACGC‑CAATTTTTGCGGCCGCCTAACACCACCAAACGGCTGGCTTTTTG‑TTCACg                                                                                                  >  1:460807/1‑239 (MQ=255)
                                                                                                                                               gatAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTg                                                                                                                    <  2:257588/219‑1 (MQ=255)
                                                                                                                                                 tAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGc                                                                                                                                                                     <  2:604164/169‑1 (MQ=255)
                                                                                                                                                  aaaGGACCCTGGTCGTCAGAGGGTTCTGATTATCCCCATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGt                                                                                            <  1:1238815/239‑1 (MQ=255)
                                                                                                                                                  aaaGGAACCGGGTCGGTAGTGGGGTCTGAGTACCACCAGTAGGCCAGGCAAGTCGACATGGCTGTTGCTGGCGGAGAGGGTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGAGAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGt                                                                                            <  1:112536/239‑1 (MQ=255)
                                                                                                                                                    aGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCCGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGGGCTGGCTTTTGG‑TTCACGAGTGGTTg                                                                                          >  2:1162436/1‑239 (MQ=255)
                                                                                                                                                        aCCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGcc                                                                                       <  2:116748/238‑1 (MQ=255)
                                                                                                                                                         ccTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGGTTTTTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTTCCGGAATTTCCGCGGTCGGCTGTAGCGGACCTTTTTC‑CGGCCAACTTTCCCGGCCGCAAAACGACACCAAAGCGCGGGCTTTTggctttctcttttaacaccac                                                                                      >  1:257588/1‑220 (MQ=255)
                                                                                                                                                          cTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTTTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGGGTGGTTGGCCTCg                                                                                    >  2:942397/1‑239 (MQ=255)
                                                                                                                                                          cTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGGGCTGGCTTTTGG‑TTCACGGGTGGTTGGCCTCg                                                                                    >  2:876314/1‑239 (MQ=255)
                                                                                                                                                                ttcggggctttctggttttcccaaTTACGGCAGTCAAGTCGGACTGGCTGTTGGTGGAGGAGGGGTTGATGGCGGGATTTTGATTTGGG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTg                                                                              <  1:175718/215‑1 (MQ=255)
                                                                                                                                                                 tcAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGGATTTCGATAAC‑GGCGAACAAGTTTGGCGGAATTCGCCCGGGCGTCTTTAGCGAACCTTTTTT‑CGCGCAACTTTTGCGGCAGCAAACAGCCCACAACGGGCTTGCTTTTTG‑TTCACAAGGGGTGGGACCCG‑aaaaggc                                                                            >  1:116748/1‑234 (MQ=255)
                                                                                                                                                                 tataagggccaggATTAGCCACTTTACGGCAGTCAAGTCGCACTGGCTGTTGGTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGc                                                                            <  2:629359/227‑1 (MQ=255)
                                                                                                                                                                     agggacaggATTATCCCCATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCaaaa                                                                        <  2:342289/231‑1 (MQ=255)
                                                                                                                                                                       cGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGc                                                                                                                                                                                                                               <  2:812687/90‑1 (MQ=255)
                                                                                                                                                                       cGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCCCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCCCGGCAGCATAACGACCCCAACGTGCTGGCTTTTGG‑TTAACGCGTGGTTGGCCCCC‑ACCTGGCAAAAAt                                                                      >  2:446668/1‑239 (MQ=255)
                                                                                                                                                                          aCTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGCGGGGTTGGCCTCG‑AACTGGCAAAAATGAt                                                                   >  2:541059/1‑239 (MQ=255)
                                                                                                                                                                          aCTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCCCG‑AACTGGCCAAAATGAt                                                                   >  2:175718/1‑239 (MQ=255)
                                                                                                                                                                                   ccACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCCACTTTCGCGGCAGCATAACGACACCAACGGGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTTTGGGTGc                                                          >  2:240849/1‑239 (MQ=255)
                                                                                                                                                                                      caTTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGGTTTTGATTTGTG‑GTACCTGCGTCGGGATTTCGATAGC‑GGCGGACAAGTTTGCCGGAATTCGCGCGGGCGTCTGTAGCGAACCTTATTC‑CGCCCAACTTTCGCGGCAGCATAACGGCCCCCACGTGCTGGCTTTTGGTTTCCCGGGT‑GTTGGGCTCC‑AACTGGCAAAAATGAtttggggtggggg                                                       >  1:836388/1‑228 (MQ=255)
                                                                                                                                                                                      caTTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACAcc                                                                                                                          <  2:160274/174‑1 (MQ=255)
                                                                                                                                                                                        ttACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTTCCGGAATTCGCGCGGGCGTCTTTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATACCGACACCCACGTGCTGGCTTTTTG‑TTTACGAGTGGGTGGCCTCT‑AACTGGCAAAAAAGaattggggggcgtggg                                                     >  1:1019218/1‑224 (MQ=255)
                                                                                                                                                                                        ttACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTTGCGCGGGCGGTTGTAGGGAACCTTATTC‑CGGCCAACCTTCCGGGAAGCAAAACGGACCCAACAGGGTGGGTTTTGG‑TTCACGAGGGGGTGGCCCCGCAACCGGaaaaaaactttgggggggggggg                                                      >  1:1203053/1‑222 (MQ=255)
                                                                                                                                                                                        ttACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCCCGAGGGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGGGGc                                                     >  2:741569/1‑239 (MQ=255)
                                                                                                                                                                                        ttACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GGACTGGCGTCGGTATTTCGATAGC‑GGCGAAACAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCCTATTC‑CGGGCCACCTTCGTCGCAGGCTACCGACACCAACGGGCTGGCTTTTGG‑GTCACGAGGGGGTGGGCTCG‑AAATGGCAAAAAAGATTTTggggtggctct                                                     >  1:342289/1‑230 (MQ=255)
                                                                                                                                                                                         tACGCCAGTCAAGTCGCACTGCCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGTTTTTTA‑GTACTGGCCGCTGCTTTTCGATAGC‑TGCGCACAAGTTTTCCGGAATTCGACCGGTCCTCTGTAGCGCACCTTCTTC‑CCCGCCACCTTTCCGGCAGCATCACGACACCCACGCGTTGGCTTTTCG‑TTCACCAATGGGTGGGCTCC‑AACTGGGAAAAAtaaatttggcagcggagag                                                    >  1:380059/1‑221 (MQ=255)
                                                                                                                                                                                              ccgtcagttggctcTGGTTTTTGCGGGGGGGGAGTTTGATGGCGGGATTTTGATTTTGG‑GTACTGGCGTCGGTATTTCGATTGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGgcg                                               <  1:615509/226‑1 (MQ=255)
                                                                                                                                                                                                gtcttgtctcatttgCTTTTGGTGGGGGGGATGTTGTTGGGGGGGTTTTGGTTTGTG‑GTAAGCGCGGAGGTAGTTCGATAGC‑GTGCAACAAGGTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGca                                             <  1:554472/225‑1 (MQ=255)
                                                                                                                                                                                                gTCAAGGCGCACTGGGTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGca                                             <  2:971158/239‑1 (MQ=255)
                                                                                                                                                                                                gTCAAGGCGCACTGGCTGTTGGTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGca                                             <  2:460807/239‑1 (MQ=255)
                                                                                                                                                                                                 ttatgtgggtgtggtttttgtgggCGGGGAGGTTGGTGGCGGGATTTTGATTTGGG‑GTTCTGGCGTCGGTGTTTCGATAGC‑GGCGAACAAGTTGGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGca                                             <  1:610896/216‑1 (MQ=255)
                                                                                                                                                                                                 ttaagtggctttgggttttGGTGGCGGAGAGGTTGATGGGGGGATTTTGATTTGTG‑GTAATGGGGGCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGca                                             <  1:291135/221‑1 (MQ=255)
                                                                                                                                                                                                 tCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACCCCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑GACTGGCAAAAATGATTGTGGATGCGTGGCTGGgcgccc                                            >  2:457956/1‑237 (MQ=255)
                                                                                                                                                                                                 tCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGGATTTCGATAGC‑GGGGAACAAGGTTTCCGGAAATTGCGGGGTCGTCTGTTACGAACCTGCTTC‑CGCGCACCTTTCCCGGCAGCCTAACGACACCAAAGCGCTGGATTTTGG‑GTCCAGCGTGGTGTGCCTCC‑AAACTGCACAAAAGATTTTggggcggggggtgggggcca                                            >  1:417745/1‑221 (MQ=255)
                                                                                                                                                                                                  cAAGTCCCACTGGGTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGcac                                            <  2:333329/238‑1 (MQ=255)
                                                                                                                                                                                                          aCTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCCCGAGTGGTTGGCCTCG‑AACCGGCCAAAATGATTGTGGATGCTTGGCTGGGCGCCCAGAACCAAg                                   >  2:1158535/1‑239 (MQ=255)
                                                                                                                                                                                                              gCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCgg                                                                                                                                         <  2:888628/135‑1 (MQ=255)
                                                                                                                                                                                                                 tgtGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGAGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGgtc                             <  2:177022/236‑1 (MQ=255)
                                                                                                                                                                                                                   tggtGGCGGAGAGGTTGATGGCGGGATTTTGATTTGGG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGgtcgtc                          <  2:1152673/236‑1 (MQ=255)
                                                                                                                                                                                                                     tgggggggggggTTTATGTGGGGATTTTGTTTTTTG‑GTACTGGGGTGGGTTTTGCGATGGC‑GGCTAAAAAGTTTGCCGGAAGTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTGGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGtcat                        <  1:825096/229‑1 (MQ=255)
                                                                                                                                                                                                                        gCGGAGAGGTTGATGGCGGGATTTTGATTTTTG‑GTACTGGCGTCGGGATTTCGATAGC‑GGCGAAAAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTAATC‑CGCGCAACTTTCGCGGCAGCATAACGCCCCCCAAGTGCTTGCTTTTGG‑TTACCGAGTGGGTGGCCTCG‑CACTGGCAAAAATTAGTTTGGGTGCAGGGCTGGGGGCCACGACCGAAGGGGGTCGTcaacaa                     >  1:954903/1‑239 (MQ=255)
                                                                                                                                                                                                                        gCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCAGCAAGACACACACGTGGTGGCTTTTGG‑TTCCCGCTGGGGTGTCCTAG‑CACCGGCAAAAATGATTTGGGATGCGTGGCTGGGCGCACACTaacaggggcggggttacaac                     >  1:1286573/1‑220 (MQ=255)
                                                                                                                                                                                                                         gggAGTGGTTGAGGGGGGGATTTTGATTTGGG‑GTACTGGCGTCGGTTTTTTGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCCAACCTTATTC‑CGCGCAACTGTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATcaa                     <  1:394079/237‑1 (MQ=255)
                                                                                                                                                                                                                           gggtgggggtgggggggTTTTTGTTTTGTG‑GGACTGGCGGTGGTTTTTCGATGGG‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGTGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATcaaca                   <  1:657763/224‑1 (MQ=255)
                                                                                                                                                                                                                                    tggggtggATTTTG‑TTTGTGTGTGCTGGGGGCGTTTTTTCGATAGG‑TGCGAACAAGGTTGCCCGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGGAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAg         <  1:240849/233‑1 (MQ=255)
                                                                                                                                                                                                                                          ggATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGGGAtt   >  2:974537/1‑239 (MQ=255)
                                                                                                                                                                                                                                           gATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCCAAAATGATTGTGGATGCGTGGCTGGGGGCCCAGTACGAAGGCGGGCGTCATCCAAAAAGCGTGGGGGGGATTa  >  2:587928/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  
CATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTG‑GTACTGGCGTCGGTATTTCGATAGC‑GGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTC‑CGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGG‑TTCACGAGTGGTTGGCCTCG‑AACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTA  >  NC_000913/4313301‑4313774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑