Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,403,906 | T→G | 16.1% | G202G (GGT→GGG) | hflC → | HflB protease modulator specific for phage lambda cII repressor |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,403,906 | 0 | T | G | 16.1% | 170.0 / 2.9 | 75 | G202G (GGT→GGG) | hflC | HflB protease modulator specific for phage lambda cII repressor |
Reads supporting (aligned to +/- strand): ref base T (3/53); new base G (15/2); total (20/55) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.90e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTGACCGTCTGCGTTCTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCGTGGTGAAGGCGATGCCGAAGCAG > NC_000913/4403670‑4404138 | tttgcccttCTGCGTTCTTAAAGTGGTCGCCTGGGCGTGAAAGATTACGTCCCCGATTCCCGTGG‑TCGGCTGAACCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTAt < 1:1296178/231‑1 (MQ=255) aCCGTCTGCGTTCTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGCGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCCGGGGATTGa > 2:400543/1‑238 (MQ=255) gTCTGCGTTCTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGGAtgtatata > 2:672844/1‑232 (MQ=255) cTGCGTTCTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGGGTTGactgtct > 2:849975/1‑233 (MQ=255) tGCGTTCTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCGGGGGATTGA‑AGTTGtg > 2:776689/1‑238 (MQ=255) ttgtGAAGTGGGTCGCCTGGGCGTGAAAGATATCGTGCCCGATTCCCGTGG‑TCGTCGGACCCGAGAAGGAGG‑GGACCGGCGGAACGCCGGTTCTGCGGGTAGAGAAG‑ATGAAGGTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGAt < 1:1234348/235‑1 (MQ=255) ttCTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGCAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGGGCCGGGAAtttggatgttgcgag > 2:623273/1‑226 (MQ=255) tCTGATATTGGGTGCCTTGCAGTGAAAAGTATCGGCAGCGATGCCCGGGG‑TCGGCTGACCCTCGAAGGACG‑GTGCGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGAtgt < 1:1048979/239‑1 (MQ=255) cTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGGGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGAtgt < 2:27591/238‑1 (MQ=255) aaTTTGTCCCCTTGCCGTGAAAGATATCGGCCCCGATTCCCTTGG‑GCGTGTGACCCTGGAAGTACG‑TGACGCGCTGAACGCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTa < 1:611318/239‑1 (MQ=255) ggTCGCCTGGACGTGAAAGATTTCGTCACCGATTCCCGGGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCaa < 2:146547/239‑1 (MQ=255) ggTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGGGCGCGTAACGGCTGAGACGAAGGGAAAAGTTCCGGCCCTCACCCCGAAAAGTATGGCGGGCGC‑GGGGGTTGA‑AGAAGTCGAGGTGCgggtaaa > 1:1273011/1‑233 (MQ=255) gTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGCCATCAACCCGAACAGATTGAC‑GGCCCCGGGGATTGG‑Aggttacgcgtgggggctcaca > 2:735703/1‑219 (MQ=255) cGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGACGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCGGGGGATTGT‑AGGTGGCGGTGTGCGGATCaaacc > 2:1266467/1‑236 (MQ=255) gCCTGGACGGTAAAGTTATCGTCACCGATTCCCGGTG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCa < 2:221624/238‑1 (MQ=255) aaaGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCACTGGGCGTTGA‑AAGTGTTGGTTTGCGGAGCCAGCAAATCAAACttc > 2:156451/1‑236 (MQ=255) tgtgctccacGATTCCCGTGT‑TCTTCTGTCTCGCCACGTTTG‑TGCCGCGCTGAGCCTCGGTTCTGCGGGTACAGAAG‑ATGAAGTTGCTCCCCCGGCGGCAGAGAGCGCCATGGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑TGCGCTGGGGATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGccgac < 1:280936/228‑1 (MQ=255) atcggccacGATTCCCGTGG‑TAGTCTGACCCTCGAAGTACG‑GTACGCGCTGAACTCCGGTTCTGCGGGTAAAGAAG‑ATGAAATTACTCCCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGccgaccga < 2:702404/231‑1 (MQ=255) accggccacGATTCCCGTGG‑TCGTCCGACCCTCTAAGTAGG‑TGCCGCGATGAACTCCGGTTCTGCGGGTTCAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGccgaccga < 2:772300/231‑1 (MQ=255) cGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGGTGGGGATTGAGAGTT‑TCGATTTGCGGCACAAGCAGATTAACCTGCCGGCCGAAggggttg > 2:279621/1‑233 (MQ=255) ttCCCGTGGTTCGT‑TGACCCGCGGAGGAGC‑TTGCGCGCGAAACTCGGGTTCTGCGGGTACAGAGGAATGAA‑TTACTGCCCAGGCGGCGAACAGCGCCATTGCCGAAGTGGCAGCGCGAGTGACGGCTGAGGCGAAGGGCAAAGTTCCGATCATCAACCCGAACAGTATGGC‑GACGCTGGGTATTGA‑AGTTGTCTATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGGAg < 1:938793/239‑1 (MQ=255) ttCCCGTGG‑TCTTTTCACCCCCGATGTACG‑TGACGCCCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTTCTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGGGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 1:1012538/239‑1 (MQ=255) ttCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:1036802/239‑1 (MQ=255) ttCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGGTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:1316457/239‑1 (MQ=255) ttCCCGTGG‑TCGTCTGACCCGCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:960830/239‑1 (MQ=255) ttCCCGTGG‑GCGTCTTACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:1059443/239‑1 (MQ=255) ttCCCGTGG‑GCCGCTGCCCCCCGAA‑TCCGATGACGCGCGTAAATCCGGTTCCGCGGGTCCAAAAG‑ATGAAGGTGCCACCCCGGCGGCAGATAACCCCAATGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 1:372730/239‑1 (MQ=255) ttCCCGGTG‑TAGTCCGACCCTCGAAGTACG‑GTACGCGCTGAACGCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:1325597/239‑1 (MQ=255) tccccGTTG‑CCGTTTGACCCTGGAAGTACT‑TGACGCGCTGAACTCCGGTTCGGGGGGTAAAGAAG‑GTGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 1:1152397/237‑1 (MQ=255) tCCCGTTG‑TCGTCTGACCCTCGAAGTCCG‑TGACGCGCTGAACTCCGGGTCTGCGGGTAAAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:557241/238‑1 (MQ=255) tCCCGTGG‑TCGTCTGACCCTGGAAGTACG‑TGACGCGCTGAACGCCGGTTCGGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:571509/238‑1 (MQ=255) tCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑GTGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:217880/238‑1 (MQ=255) tCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:945741/238‑1 (MQ=255) tCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:1273011/238‑1 (MQ=255) tCCCGGGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTCCCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:485089/238‑1 (MQ=255) tCCCGGGG‑TCGTCATCCCCTCGGAGTACG‑TGACGCGCTGAACCGCGGTTCTGCGGGTAAAGAAG‑GTGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 1:800402/238‑1 (MQ=255) gcggTGG‑TCGTCTGACCCTCGAAGGACG‑GGACGGGCTGAACTCCGGTTCGGCGGGGACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCg < 1:941/236‑1 (MQ=255) cccGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCg < 2:1303440/239‑1 (MQ=255) tcgtcTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTAc < 2:1000883/238‑1 (MQ=255) tGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGGATTGA‑AGGTGGCGGTGGGCGGATCAAGCAGATCAACCTGCCGCCCGCAGTGTCTGGAGGGATCTACAAcca > 2:941/1‑237 (MQ=255) cTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCCCTGGGGATTGA‑AGTTTTCGATGTGCGGTTCAAGCAGATCAACCTGCCCACCCCAGGGTCTTAAGGGATCTACAACCCTATgc > 2:544783/1‑238 (MQ=255) aaatacttgacgccctggctgccGGTTGTGCGGGTACAGAAG‑GTGAAGTTGCTACCCCGGCGTCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCACAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGcc < 1:802689/218‑1 (MQ=255) tACG‑TGACGCGCTGAACTCCGGTTCGGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAg < 2:1260084/213‑1 (MQ=255) ggccccgtggtcttcggtttcgggggTACAGGAG‑ATGAAGTTACTCCCCCGGCGGCAGATAACCCCATGGCCGAAGCGGCAGGGCTGATAACGGCGGGGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGGATGGC‑GGCGCTGGGTATGGA‑AGTTGGCGAGGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTg < 1:685541/215‑1 (MQ=255) ttCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGcgtcgt < 2:785457/238‑1 (MQ=255) ttctgcggggtcaagaagggAAGGTACGCCCCCGGCGGGAGATAACGGCATTGGCGAAGCGGCAGGGCGAGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCAGCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGcgtcgt < 1:143044/219‑1 (MQ=255) tatGCGGGGAAAGAAG‑ATGAAGTTACTGCCCCGGCGGCAGATAACGCAGTTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCAGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCa < 2:549424/237‑1 (MQ=255) aCAGAAG‑ATGAGGTTACTCCCCCGGCGGGAGATAGCGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGAGCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTcaca < 1:68182/239‑1 (MQ=255) aCAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCCCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTcaca < 2:695937/239‑1 (MQ=255) aagaag‑aTGCAGTTACCACCCTGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGGAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGACCAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTcaca < 2:703374/237‑1 (MQ=255) gcgagggggggttttggtccccTGCGGGAGATAACGCAATTGCCGAAGCGGCAGGGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGGCATCAACCCGATCAGTATGGC‑GGCGCTGGGGATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCATCCTGGCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTcaca < 1:169016/220‑1 (MQ=255) gaag‑aTGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGG‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGGGGCTGAAGGGACCTACAACCGAATTCGGCCCGACGGTGAAGCGGGAGACGGTCGTCACCCTTccccacg > 2:113963/1‑233 (MQ=255) aaatgaattaccccccccGGCGGCAGATAACGCCTCTGCCGAAGCGGAAAGGCGCGGAACGGCTGAGACGTAGGGGAAAGTTCCGGTCAACAACCCGAACAGTCCGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGGGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGAGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGt < 1:1226664/224‑1 (MQ=255) gAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGGATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGAGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGAGTGAAACGGGAGCGCGTCGTGACCGTTCACAAGGTCAGga > 2:390853/1‑239 (MQ=255) gcggcAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGGATTGT‑AGGTGTCGGCTGGCGTCTCAAGCAGATCAACCCGCCGCCCGACGTGGCTGCTGCGGTCTCTAACCCCAAGCGCGCCACGCGAGAAGAGCGGGAGCGCCGTCACCATCCACAAgcgcggcgagaaaagggaaaaaa > 1:1088414/1‑217 (MQ=255) cgcatacggCCCTTGCCCAAGCGCGAGGGCGCGTAACGGCGTGGACGAAGGGCAATGTCCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATGGA‑AGTTGTCGATGTGCGTAGCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTgcg < 1:978876/230‑1 (MQ=255) gCCGTTGCCTAAGCGGCAGAGCGGGTAACGGGTGAGACGATGGGCAAGGTGGCGGCACGCACACCGAACAGTATGGC‑GGCGCTGGCTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGGCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGc < 1:735703/239‑1 (MQ=255) ttGCCGAAGGGGCAAGGCGCTGAACGGCTGATACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGAc < 2:233351/239‑1 (MQ=255) ttGCCGAAGCGGCAGAGCGCGTACCGGCTGATACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGAc < 2:184313/239‑1 (MQ=255) tGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGGATTTA‑AGTTGTCGATGTGCGTATCAAACAGATCAACCTGCCGACCGAAGGGTCTGAAGCGATCTACAACCGTATGCGCCCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCTGGA‑AGAAACGGGAAAACTGCCCCCGACTGCCgg > 2:991013/1‑236 (MQ=255) tGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCCGTGTGGCTTGA‑AGTTGTCGACGTGCGTTTCAAGCAGAACAACCTCCCGCCCGTAGAGTCTGAGGCGATCTACAACACTCTGGTGCCCCAGCGTGAAGAGGGAGGGGCGCGGCACCCGTCACAAGGTTACGAGAAACGCGGaaaaagacgccgcgcccgcgccc > 1:1242877/1‑221 (MQ=255) tGCCGAAGCGGCAGAGCGCGTAACGGCTGAAACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACt < 2:1242877/239‑1 (MQ=255) agagCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAACAGATCAAACTGCCGACCGAAGTGTCTCAAGCGATCTACAAACCGATGGGCGCCCGGCGTGAAAGGGTAGCGGGGCGGCACCCTTCACAAGGGTAGGG‑AGAGACGGGAAAAATGCGCGCGACCGCCGACCCAGAAGGGACCa > 1:332909/1‑239 (MQ=255) cgcgTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTcac < 2:783770/181‑1 (MQ=255) ccttagcGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCGGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAGCCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACg < 1:1301808/233‑1 (MQ=255) cGGCTGAGACGAAGGGGAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGGTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCa < 2:897820/239‑1 (MQ=255) cGAAGGGCAAAGTCCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTAGTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTg < 2:784450/237‑1 (MQ=255) ggggCACTGTTCCGGACATTAACCCAACCAGGTGGGG‑GGCGCTGGCTATGTA‑TGTTGTCGATGTGCGTATGAAGCAGATCAACCTGCCGACCGAAGTGTCTGACGCGCTCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGt < 1:828417/238‑1 (MQ=255) ggCAAAGTTCCGGTCATAAACCCAAACGGTAGGGC‑GGCGCTGGTTATGTA‑AGTTGTGGGTGTGCGTAACAAGCACACAAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGt < 1:1135909/237‑1 (MQ=255) aagggccagTCATCAACCCGAACAGGTTGGG‑GGCGCTGGGTATTGA‑AGTTTTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGc < 1:16083/231‑1 (MQ=255) cGGTCATCAACCCGAACAGTATTGC‑GGCGCGGGGTATTGA‑AGTTGTCGATGGGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGcatc < 2:1215676/239‑1 (MQ=255) aaCAGTATGGC‑GGCGCTGGGGATTTA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGGCAGGA‑AGAAGCGGGAAAACTGCGCGCGGCTGCCGACTATTAAGGGACCAGAAAGCTGGCAGAAGCTGAGCGTCAGGGGCGCATCATgg > 2:16083/1‑228 (MQ=255) tATGGC‑GGCGATGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGc < 2:594140/217‑1 (MQ=255) cgcTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGGGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGCCTGCCGGCTATGAAGTGACCCAAACGCTGGCAGAAGCTAAGCGCCAGGGCCCCACAATGCGTGGGGAAGGCGATGCCGaaca > 2:847007/1‑236 (MQ=255) cTGGGTATTGA‑AGTTGTCGATGTGCGTAACAAGCAGATCAACCTGCCGACCGAAGTGTCGGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCGTGGTGAAGGCGATGCCGAagcag < 2:864272/238‑1 (MQ=255) | TCTGACCGTCTGCGTTCTGAAATTGGTCGCCTGGACGTGAAAGATATCGTCACCGATTCCCGTGG‑TCGTCTGACCCTCGAAGTACG‑TGACGCGCTGAACTCCGGTTCTGCGGGTACAGAAG‑ATGAAGTTACTACCCCGGCGGCAGATAACGCCATTGCCGAAGCGGCAGAGCGCGTAACGGCTGAGACGAAGGGCAAAGTTCCGGTCATCAACCCGAACAGTATGGC‑GGCGCTGGGTATTGA‑AGTTGTCGATGTGCGTATCAAGCAGATCAACCTGCCGACCGAAGTGTCTGAAGCGATCTACAACCGTATGCGCGCCGAGCGTGAAGCGGTAGCGCGTCGTCACCGTTCACAAGGTCAGGA‑AGAAGCGGAAAAACTGCGCGCGACTGCCGACTATGAAGTGACCAGAACGCTGGCAGAAGCTGAGCGTCAGGGCCGCATCATGCGTGGTGAAGGCGATGCCGAAGCAG > NC_000913/4403670‑4404138 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |