Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,998 C→T 17.3% S45F (TCT→TTT)  cycA → D‑alanine/D‑serine/glycine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9980CT17.3% 141.3 / 5.9 72S45F (TCT→TTT) cycAD‑alanine/D‑serine/glycine transporter
Reads supporting (aligned to +/- strand):  ref base C (19/39);  new base T (9/4);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 2.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCT  >  NC_000913/4429771‑4430236
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
attccGATTAGTCCCGATACAAATTCGTCGTATCATAGACTGAACAAAGGCCGTAGATCCTGAACAACACATACAGGTAAAGGAAGAAAAAACCATGGTAGATCCGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGa                                                                                                                                                                                                                                           <  1:270935/235‑1 (MQ=255)
      aTGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCATTGGGAACGGGGTGGTTTATGGGGCTGGCaaaaagtatta                                                                                                                                                                                                                                       >  2:359873/1‑230 (MQ=255)
           tCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCATTGGACGGGGGTGTTTTTGTGGGTTTGCAAAAAGAATAGcccttcc                                                                                                                                                                                                                                >  1:1029941/1‑234 (MQ=255)
            cccGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCg                                                                                                                                                                                                                               <  1:259410/239‑1 (MQ=255)
                      aTTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTTGGGGCTTGCAAAACAATTAGCCTTTCCGGGCCGGCGac                                                                                                                                                                                                                     >  2:1100964/1‑238 (MQ=255)
                      aTTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTAGCTACGGCGCAATCTCACAAACCGACATATTCAGCTAATTGCCATTGGCGGAGCGCTTTGTATGGGGGTGGTTTTTGGGTCGGGGAAAACAAGTAGACCTGCCCGGGCgggggc                                                                                                                                                                                                                     >  1:883964/1‑234 (MQ=255)
                           tcgtATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGTTGTTTATTGGGTCTTGCCAAACGATTTGCCCTTCCGGGGCGTCGATGAttt                                                                                                                                                                                                              >  2:1099334/1‑239 (MQ=255)
                           aggttacATAGACTGAAAAAAGGCGGGAGAGCCTGAACAACAAAGACAGGTAAAGGAAAAAAAAAACTGGGTAGTACCGGTAAAAGGCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGTTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                                                                                                                                                                                               <  1:9754/232‑1 (MQ=255)
                            aacaTCATAGAATGATAAAAGGCCGTAGAGCCTGAAGAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGAGCAGGTAAAAGTCGTTGCCGATGACCAGGCTCCGGCTTAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttt                                                                                                                                                                                                              <  1:23695/235‑1 (MQ=255)
                                tCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTAAGGGGGTGGTTTTGGGGGTTGGGAAAACAATTAACCTTTCCGGGCCGTCGagaatattttgt                                                                                                                                                                                                           >  2:230845/1‑228 (MQ=255)
                                    aGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                                                                                                                                                     <  2:1085544/239‑1 (MQ=255)
                                      actgactAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATg                                                                                                                                                                                                   <  2:249283/239‑1 (MQ=255)
                                        tgactAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACTGGGGTGTTTATTGGGGCTGGGAAAACGATTTACCCTTCCCGGCCGCCGCTCATTTTTGTTTttatctt                                                                                                                                                                                                 >  2:9754/1‑236 (MQ=255)
                                          actAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCATTGGTAAGGGGTTGGTTATGGGGGCTGGCAAAACGATTATCCTTGCCGGGCCGGCGATTCTTTTCGGTTatatatgca                                                                                                                                                                                               >  2:145966/1‑234 (MQ=255)
                                          actAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGTACGGGGTTGTTTTTGGGGTTCTGCAAAACAATTAGCCTTACCGGGCCGTCGGTCGCTTTCTTTTttatattca                                                                                                                                                                                               >  2:292096/1‑234 (MQ=255)
                                          actAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTGGGAAGGGGGTGTTTTTGGGGTTTGGCAAAAACATTAGGCTTTCCGGGCCGGGCGTCATTTTCGGTTATTTgcttt                                                                                                                                                                                               >  2:23695/1‑235 (MQ=255)
                                          actAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAAACGACATATTCAGCTTATTGCCATTGGCGGGTGCATTTGTACGGGGGTGGTTTTTGGGGTTGTGAAAAACAATGTGCTTTGCCGGGCCTGGGTCATTTTTGttttctttaaca                                                                                                                                                                                               >  1:1044462/1‑230 (MQ=255)
                                           ctAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                                                                                                                                            <  2:883964/239‑1 (MQ=255)
                                           acaaaagccgtgaggCCTAAACAACAACGCCAGGTACAGGAAAAAAAAAACATGGTGGATCCGGTACAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAAGCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTTGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                                                                                                                                            <  1:1130092/225‑1 (MQ=255)
                                           aaaaaggcccgaagtctggAACAACCAACACAGGTAAAGGAAGAAAAAAACATGGGAGGTCAGGTAAAAGTCGTGGCCGATGATCGGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                                                                                                                                            <  1:1099334/221‑1 (MQ=255)
                                           aaaaaGGCCGTAGGTCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACAGGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATACTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                                                                                                                                            <  2:737964/237‑1 (MQ=255)
                                                    gTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTGCTTATGGGGTCTGGCAAAACGATTTACGTTGCCGGGCCGCCGATACTTTTCGTTTTAATAGTCATTTGttttta                                                                                                                                                                                   >  2:751691/1‑237 (MQ=255)
                                                    gTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTGGGACGGGGGTGTTTTTGGTGGTTGGCCAAACAACTAGACGTGTCGGGGCCCGGGTCATTTTCGttttatagaagcttggttgttt                                                                                                                                                                                     >  1:968533/1‑220 (MQ=255)
                                                    gTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGCAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGTTACGGCGCAATCTCACCAACCGAGATATTCCGATTATGGCCATTGGCGGTGCCACTGGTTTCGGGGGTGTTTTGAGGGCTGGCACAAAGATTTGCCATTCCGGGGCGTGGAGCCGTTTTTGTTTTTTTATCATTTGttttta                                                                                                                                                                                   >  1:910751/1‑237 (MQ=255)
                                                                   cacaGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCCTTTGATCGGGGTTGGTTATTGGGGTTGGCCAAACGGTTTACCCTGGCCGGTCGGGGGTCCTTTTTGTTTTTATAATTATTTGGTTTTTTGTTTTTTTTAGtat                                                                                                                                                                     >  1:119709/1‑237 (MQ=255)
                                                                    acgaaagggtcaatgaaaaaAAAAACCGGGGAGAAAAGGTAAAAGGCTTTGGCGAGAATCAGGCTCCGGCTGAACAGTCGCTGCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATg                                                                                                                                                                   <  1:608782/225‑1 (MQ=255)
                                                                        acagGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTAACGGGGTGTTTTTGGGGGCTGGCCAAAAGATTAGCCTTTCCGGGGCGGCGGTCCTTTTCGTTTTTATGATTATTGGGTTTATGCTCTTTTTTGTGATGCgg                                                                                                                                                                >  2:55174/1‑238 (MQ=255)
                                                                          agGTACAGGAAGAAAAAAACATGGGAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCa                                                                                                                                                             <  2:634803/239‑1 (MQ=255)
                                                                             tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGTTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGc                                                                                                                                                              <  2:398454/235‑1 (MQ=255)
                                                                                      aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCCAAACGATTAGCCTTTCCGGGCCGTCGGTCATTTTTGTTTTTTTGATCTTTGGGTTTTTTCTCTTTTTTGTGCTTCGGGGAATTGGGGGAttt                                                                                                                                                 >  2:1294739/1‑238 (MQ=255)
                                                                                       aaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTTGTACGGGGGTGTTTTTGGGGACTGGCAAAAAGTCTAGCCCTGCCGGGCGCTGGCTCTATTTTCTTTTTATTATGAGTGGGTTTATTCTCTTTTTTGTTGTGCGGGCACAggggggtaggg                                                                                                                                                >  1:385104/1‑233 (MQ=255)
                                                                                                gggaGATCAGGTAAAAGGCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGa                                                                                                                                       <  2:644528/236‑1 (MQ=255)
                                                                                                      tCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCCCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTg                                                                                                                                  <  2:1102601/238‑1 (MQ=255)
                                                                                                       cAGGTAAAGGTCGTTTCCTATTATCGGGGTCGCGTTGAAACGCTGCTACGGCGCATACTCACAACCCTACCTATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTg                                                                                                                                  <  1:751691/237‑1 (MQ=255)
                                                                                                        aGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTAAGGGGGTGTTTTTGGGGGCTGGCAAAAAGAGTAGCCCTGCCCGGCCGGCGGCCATTTTTGGTTTTATGATCATTGGTTTTTTGTCCTTTTTTTTGTTGCGGGCAAAGGGGGGATTGTTGATTTTGaaatttgaa                                                                                                                               >  1:644528/1‑232 (MQ=255)
                                                                                                             aaaggctgtGCCGATGAACAGGCCCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa                                                                                                                         <  2:1326915/231‑1 (MQ=255)
                                                                                                             aaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa                                                                                                                         <  2:1029941/239‑1 (MQ=255)
                                                                                                                    ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGTACGGGGCGGTTTTTTGGGTTTTGCCAAACCGCTTGCCTTGCCCGGCCGCTGACGGTTTTTGTTTGTATCATCACTGGTTTTATGCCCCTTTTTGTGATCCGGGATATGCGGGAATTGTGGCTTTTTACTCTGGCATACAAACCtttt                                                                                                                  >  1:194983/1‑239 (MQ=255)
                                                                                                                    ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGTAGGGGCGGTTTTTGGGGGGTGTCGAAACATATAGCCTTGTCGGGGCGGCGATCTATTTTGTTTTCATGTTCATTTGTTTTTTGCTTTTTTTTTTGTTTCGGGCCATTGGGGGATTTGTGCGTTTGGATCTgtgaaagaaaacattt                                                                                                                   >  1:1326915/1‑224 (MQ=255)
                                                                                                                                 ggCTCCGGCTGAACAGTCGGTACGGGACAATCTCACAAACCGACATATTCAGTTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCtt                                                                                                    <  2:176564/239‑1 (MQ=255)
                                                                                                                                     ccGGTTGAAAAGTCGTTACGGGCCAATCTCACAAACCGGTATATTCAGGTTATTGCCATTGGGGGGGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGa                                                                                                <  1:55174/239‑1 (MQ=255)
                                                                                                                                      ctgtgtaaCGGTCCCTTCGGCGCAATCTCACAAACCGACATGTTCAGCTTATGTCCATTGGCGGTGCCATTGGTGCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGAt                                                                                               <  2:792496/233‑1 (MQ=255)
                                                                                                                                      cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGGACGGGGGTGTTTTTTGGGGCTTGCAAAACGAATATCCTTTTCCGGGGGTCGGTCATTTTTGTTTTTATTGTGATTTGTTTTTTGCTCTTTTTTGTGATTCTGGCGATGGGGGGATTGTTTTTTTTTTATTTGGGATAAAAAACTTTTTGttgcttgtgttctgat                                                                                                >  1:610013/1‑224 (MQ=255)
                                                                                                                                           gAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTTTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACt                                                                                          <  2:968533/239‑1 (MQ=255)
                                                                                                                                            gacagtgtgttcGGCGCAATCCCCCAAACCGGCATATTTAGCTTATTTGCATTGGGGGGTCCATTGGTGCGGGGTTGTTTTGGGGGTCTGGGAAAACGATTAGCCTTGGCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTc                                                                                         <  1:743173/228‑1 (MQ=255)
                                                                                                                                             aCAGGCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTc                                                                                         <  2:1044462/238‑1 (MQ=255)
                                                                                                                                                  cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGcc                                                                                    <  2:803210/238‑1 (MQ=255)
                                                                                                                                                               tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTGTTTATGGGGGCTTGCAAAAAGATTAGCCTTTCCGGGCCGTCGGTTATTTTTGTTTATTATATTATTGGTTTTTTTGTCTTTTTTGTGGTTTGGGGCATTGGGGAATTGTTGCTTTTTGATCTGGTGATAAAAACTTTTTTTGGTTTCGCTTCCGTTTTATTTCGGGCGTggggggggaatt                                                                      >  1:465131/1‑230 (MQ=255)
                                                                                                                                                               tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGACATTGGTACGGGGGTGTTTTTGGGGGTTGGCAAAAAGGTTAAGCTTTGCGGGCCGGCGTTCATTTTCGTTTTTATGATCCATTGGTTTTTTCTCTTTTTCGTGATGTGGGCCATGGGGGGATTGCTGCGTTTGTATTTGGTATACAAAACTTTTTTTGACTTTGCGTTCCGTTTACTTGAGCCGGGGGCAGGAGAtt                                                                      >  1:173164/1‑239 (MQ=255)
                                                                                                                                                                      aaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCgg                                                               <  2:1222621/239‑1 (MQ=255)
                                                                                                                                                                       aCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGc                                                              <  2:202196/239‑1 (MQ=255)
                                                                                                                                                                            tattttccgtttATTGCCTTTGGGGGGGCCATTGGTACGGGGTTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTAGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCAGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGa                                                          <  1:600457/228‑1 (MQ=255)
                                                                                                                                                                              tatTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGAt                                                                                                                                                                    <  2:997468/132‑1 (MQ=255)
                                                                                                                                                                                tcttgtttttttccATTGGCGGTGCCATTGGTTCGGGGTTGTTTATGGGGTTTGGCAAAACGATTGGCCTTGCCGGGCCGTCGATCATTTTCGTTTATAGGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTAc                                                     <  1:1100964/227‑1 (MQ=255)
                                                                                                                                                                                         ttGCCTTTTGCGGTGTCATTGGTGGGGGGTTTTTTATGGGGTCTGGAAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTctgc                                            <  1:359873/239‑1 (MQ=255)
                                                                                                                                                                                         ttGCCATTGGCGGTGCCATTGGTACGGGTTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTctgc                                            <  2:610013/239‑1 (MQ=255)
                                                                                                                                                                                          ttctgttGGTGGGGCCATTGTGAGGGGGTTGTTTATGGGGTTTGGCAAAACTATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATTATTGGTTTTATGCTCTGTTTCGTGATGGGGGGAATGGGGGAATTGCTGCTTTCGAATTTGGAATAGAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTctgct                                           <  1:292096/234‑1 (MQ=255)
                                                                                                                                                                                          tGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTctgct                                           <  2:385104/239‑1 (MQ=255)
                                                                                                                                                                                            ccATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGc                                                              <  2:728866/218‑1 (MQ=255)
                                                                                                                                                                                               ttGGCGGTCCCATTGGTTCGGGGTTGTTTATGGGGACTGGCAAAACGATTAGCCTTGCCGGGCCGTGGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCGTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCTGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGtt                                      <  2:431681/239‑1 (MQ=255)
                                                                                                                                                                                                   cGGTGCTATTGGGACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATAGGATCATTGGTTTTATGCCCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTa                                   <  2:910751/238‑1 (MQ=255)
                                                                                                                                                                                                   cGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTa                                   <  2:119709/238‑1 (MQ=255)
                                                                                                                                                                                                    ggTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTaa                                  <  2:1154227/238‑1 (MQ=255)
                                                                                                                                                                                                    ctgtcccATGGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATTATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTa                                   <  2:5238/233‑1 (MQ=255)
                                                                                                                                                                                                                    cGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGTTATTTCCCCGGCTGGACTTACTTGTTTTGCTGGGTTGTAAACGGTATGGgagaa                    >  1:621713/1‑238 (MQ=255)
                                                                                                                                                                                                                      ggggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCGGACg                   >  2:605724/1‑239 (MQ=255)
                                                                                                                                                                                                                        ggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACgtg                 >  2:1186217/1‑239 (MQ=255)
                                                                                                                                                                                                                        ggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCCCTTCCGATTTATTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTCATGGGTCTGCTGGGTTGTCACCGGTATGGCAGAccgg                 >  2:1274760/1‑236 (MQ=255)
                                                                                                                                                                                                                            gttTATGGGGTTTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATCGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGtggtgg             <  2:490106/239‑1 (MQ=255)
                                                                                                                                                                                                                             tttATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGGGGc            >  2:1154529/1‑239 (MQ=255)
                                                                                                                                                                                                                                   gggTTTGGCAAAACGATTAGGCTTGCGGGGTCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGGGATGCGGGCAATGGGGGAATTGGTGCTTTCGAATCTGGAATACAAAGCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCAc      <  1:1294739/239‑1 (MQ=255)
                                                                                                                                                                                                                                    ggTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTGTTCGGGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCAc      <  2:362593/238‑1 (MQ=255)
                                                                                                                                                                                                                                       cTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTaa                                  >  2:740318/1‑207 (MQ=255)
                                                                                                                                                                                                                                       cTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTAACCGGCTGGACTTCCTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCt  >  2:372008/1‑239 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
ATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCT  >  NC_000913/4429771‑4430236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: