BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,444,675	T→G	13.5%	V188G (GTG→GGG)	tamB →	translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,444,675	0	T	G	13.5%	309.8 / 2.6	161	V188G (GTG→GGG)	tamB	translocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand): ref base T (56/69); new base G (23/9); total (83/78)
Fisher's exact test for biased strand distribution p-value = 9.35e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

TGCCTCCTTCTGAACAGGTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGG  >  NC_000913/4444441‑4444906

tGCCTCCTTCTGAACAGGTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCCCGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGACGAAAACCCTGACCCTGAAACCGACGTCCCGGAAGGGCCGGCGGATTGCCTTGCCGAAA‑GGGGGGG‑AAGGGgcg                                                                                                                                                                                                                                            >  1:503205/1‑239 (MQ=255)

tGCCTCCTTCGAAACGGGTTGAAGAAGAGGAAGATAGCGGTCCGTGGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGgcg                                                                                                                                                                                                                                            <  2:20710/239‑1 (MQ=255)

            aaCAGGTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTTTGCCGAAA‑GTGGCGG‑CAAGGGGGCCGGGAGAAGTg                                                                                                                                                                                                                                >  2:806210/1‑239 (MQ=255)

                 gTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCCACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGCAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGGCTGCTGATTGCTCTGCCCGAA‑GTGGCGG‑AAGGGGCGCCGgacgagaggggcga                                                                                                                                                                                                                           >  1:244996/1‑230 (MQ=255)

                 gTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCACTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AGAGGGGGCAGGAAAAAggggggga                                                                                                                                                                                                                           >  2:873643/1‑235 (MQ=255)

                           agGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCCACGTCGCTGAAAGGCCTGCTGATTGCTCTCCCCAAA‑GGGGCGG‑AAAGGGCGCAGAGAGAAAGGGGG‑GAACCCAAAAta                                                                                                                                                                                                                >  1:786656/1‑238 (MQ=255)

                                         ccGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACCCGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGc                                                                                                                                                                                                   <  2:747340/238‑1 (MQ=255)

                                          cGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAACACCCTGACCCTGAAACAGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGGGGCGCAGGAAGCAGTGGGC‑GAACCCGAAATTGAAAATCCGAAGACg                                                                                                                                                                                                 >  2:887529/1‑239 (MQ=255)

                                            cTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGCCGG‑AAGTGGGGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGa                                                                                                                                                                                               >  2:214808/1‑239 (MQ=255)

                                                    ctcCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTACGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGCAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGCAGAATTGTGC‑GAACCGAACATTGAAAATCCCAAGCCGGG‑TGaaaaa                                                                                                                                                                                       >  2:558577/1‑237 (MQ=255)

                                                    ctcCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGGATCGGTGATGGACTTCACCTCCGGCCTGAACTAGCAGGAGAAAACCCTGACCCTGAAACCGACGGCGCTGCAAGGCCTGGTGATTGCTCTGCCGGAA‑GAGGCGGCAAGGGGGCCAGAAGGAAG‑GGGG‑GAACCGGAAATTGAAAATCCGCAACCGGA‑GAaaaaa                                                                                                                                                                                       >  1:914136/1‑237 (MQ=255)

                                                      ccACGCCGTATCCCATCCCCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCg                                                                                                                                                                                    <  2:210001/239‑1 (MQ=255)

                                                         cGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑TAGGGGGCCAGGAAGAAAGGGCC‑GAACCGAAAATTGAAAAACGGCacacggcctgaaagcgcctc                                                                                                                                                                                  >  2:97869/1‑220 (MQ=255)

                                                         cGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAAAAGTGGTC‑GAACCGACAATTGAAAAACCCCAGCCGGA‑TGAAAAGCCGccc                                                                                                                                                                                 >  2:474284/1‑237 (MQ=255)

                                                            cGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGAACCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTCCCGAAA‑GTGGCGG‑AACTGGCGCAGGAATAAGGGAGC‑GAACTCAAAATTCAAAAACCCCCGCCCGA‑CGCAAAGCTGccctgc                                                                                                                                                                              >  2:644854/1‑234 (MQ=255)

                                                               tgaccATCCACCCGACACGGGTGGCACTGGAAAACGTCAACATGAAGATGGATGGCACAGCGGTATCGGTGATGGACTTCACCGCCGGCCTGAACGGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCGCGGCGaa                                                                                                                                                                           <  1:146967/236‑1 (MQ=255)

                                                               aTCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGGC‑GAACCGAAAATTGAAAATCCCAAGCCGGA‑TGAAAAACCGCTCGGCgga                                                                                                                                                                           >  2:305954/1‑237 (MQ=255)

                                                                   cATCCCCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGc                                                                                                                                                                       <  2:363568/239‑1 (MQ=255)

                                                                      cACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAACCCCTCGGCGAAACGCTGa                                                                                                                                                                    >  2:427427/1‑239 (MQ=255)

                                                                      cACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGGGGCGCAGGAAGAAGGGGTT‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAACCCGCCGGCGAAACGCTGa                                                                                                                                                                    >  2:1130223/1‑239 (MQ=255)

                                                                         ccTGACGCGGGTGGCACTGTACAACGTCAACATTAAGATTGATGTCTGCACGGTATCGGTGATGGACTTCACCACCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAg                                                                                                                                                                 <  2:615913/239‑1 (MQ=255)

                                                                           tGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGCAAATTTAAAATCCGCCGCCGGA‑TGAAAAACCGCCCGCCGGAAAGCTGGAAGa                                                                                                                                                                >  2:989253/1‑238 (MQ=255)

                                                                           tGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AACGGGCGCAGGAAGAAGTGGTC‑GGACCGGAAATTGAAAATCGCCAGCCGGAGTGAAAA‑CCGCCCGGCGAAAACCGGAAAGa                                                                                                                                                                >  2:1273361/1‑238 (MQ=255)

                                                                                   gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTTAAAATCCGCAGCCGGA‑GGAAAAGCCGCTCGGCGAAACCCTGaaaacatccttt                                                                                                                                                         >  2:754747/1‑228 (MQ=255)

                                                                                   gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGGAAATCCGCAGCCGGA‑TGAAAAGCCGGCCGGCGAAACCCTGAAAGATCTCttt                                                                                                                                                         >  2:86703/1‑237 (MQ=255)

                                                                                   gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCttt                                                                                                                                                         >  2:1169415/1‑237 (MQ=255)

                                                                                   gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACCCTGAAAGATCCCcttt                                                                                                                                                        >  2:430968/1‑238 (MQ=255)

                                                                                         ctggaacactgcaaacttAAGGTTGATGACACCACTGTCTCGGTGATGGACTTCACCTCCGGCCTGAACGGGCAGGAGAAAACCCTGACCCTGAAACCGACGGCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGccc                                                                                                                                                 <  2:140927/223‑1 (MQ=255)

                                                                                         cgggACAACGTCAACATTAAGATTGAAGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGcc                                                                                                                                                  <  2:918875/236‑1 (MQ=255)

                                                                                            gACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGAGGCGCCGGAAGAAGGGGGC‑GGACCGAAAAATGAAAAACCCCACCCGGG‑GGAAAAACCCCCCGGGGAAAAACGGAAAAATCCCTTTTCTCGccccga                                                                                                                                              >  1:1064433/1‑236 (MQ=255)

                                                                                                 cGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGCCGTCGCTGACAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑CAGGGGGCCAGGGAGAAGGGGGCGGAACCGAAATTTAAAAAACC‑CAGACGGA‑GGAAAAGCCCGCCGGCGAAAAGCTACAAAAACTCTTTTTTCCCCCGGGAtttc                                                                                                                                         >  1:763668/1‑237 (MQ=255)

                                                                                                 cGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGCAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGGGAAACGCTGAAAGAACCCTTTTTTCGCCCGGTATTGc                                                                                                                                         >  2:13182/1‑239 (MQ=255)

                                                                                                 cGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGGGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCCCGGCGAAAAGCTGGAAAATCGCTTTTCTCGCCCGGTAttt                                                                                                                                          >  2:106871/1‑237 (MQ=255)

                                                                                                      aCATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCCGaa                                                                                                                                    >  2:1102506/1‑239 (MQ=255)

                                                                                                            aGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAAAGCTGGAAAATCCCTTTTCTCGCCCGGGCTTGCCGGGAATGAcc                                                                                                                              >  2:113306/1‑239 (MQ=255)

                                                                                                                 gatgaCACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGTTTGCTCTGCCGAAA‑GTGGCGG‑AAGTGgcc                                                                                                                                                                                                                                            >  1:571177/1‑125 (MQ=255)

                                                                                                                  atgaCACCACGGTATCGGTGCTGGACTTCACCTCCGGCCTGAACTGGCAGGCGAAAACACTGACCCTGAAACCGACGTCGCTGAAAGGCATGCTGATTGCTCTGCCGAAA‑GTGGCGG‑CAGGGGCGCCAGAAGAAGTGGTC‑GGACCGGCAATTGACAACTCCCAGGCGGA‑TGAAAAAGCGGCCGGCCAGACGGCGAAAGAAGTCCTTTTTCGCCCGGttgtgaccgacatgaacggccgg                                                                                                                        >  1:1264730/1‑217 (MQ=255)

                                                                                                                   tgaCACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGGGCAGGAAGAAGTGGGC‑GAACCGAAAATTGAAAATCCGAAGACGGC‑TGAGAAAGCGCCCGGCGCAACGCTGGAAGACTCCCTTTCTCCCCCCGAGTTGCCGGaaacaaagcgcgtgc                                                                                                                       >  1:317579/1‑227 (MQ=255)

                                                                                                                        ccacGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AGGTGGCGGAGGAAGAAGTGGTC‑GAACCGACAATTTAAAATCCGCAGCCGGG‑TTAAAAGCCCCTCGGCGAAACGCTTAAAGATCTCATTTCTCTCCCCGGAATGCAGGAGATGAccacgacgcattt                                                                                                                   >  1:318408/1‑227 (MQ=255)

                                                                                                                            ggTAGCGGTGATGGTCTTCACCTCCGGCCTTAACTGGCAAGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTTCTTATTGCTCTGCCGAAA‑GTGGCGT‑AAGTGGCGCAGTAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGc                                                                                                             <  2:841596/239‑1 (MQ=255)

                                                                                                                              tATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GCGGCGG‑CAGAGGCGCAGGAACAAGTGGTC‑GCACCGCCAATTGAAAATCCCCAGCCGGA‑TGAAAAAACGCTCGGCCAAACGCTGAAAAAACCCTTTTCTCGCCAGCGTTTACCCGAAAAGACCGAC‑CGGCATTTGcccgct                                                                                                           >  1:1301568/1‑235 (MQ=255)

                                                                                                                                 cGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGGACATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCGCGGCGAAACGCCGAAAAATCTCTTTTCTAGCCCGGTATTGCCGGAAATGACCGCC‑GTGCATTTGCCCCTTaa                                                                                                         >  2:664731/1‑238 (MQ=255)

                                                                                                                                   gTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTtc                                                                                                                                                       >  2:968056/1‑191 (MQ=255)

                                                                                                                                   gTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTtc                                                                                                                                                       <  1:968056/191‑1 (MQ=255)

                                                                                                                                    tGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTTAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCt                                                                                                      <  2:485824/238‑1 (MQ=255)

                                                                                                                                      aTGGGCTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCCCGGCGAAACGCTGAAAGATCTCTTTTTTCGCCCGGGATTGCCGGAAATGACCGCC‑GGGCATTTGCCGCTTAACCTG‑aa                                                                                                  >  2:162360/1‑239 (MQ=255)

                                                                                                                                      aTGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTTTCCCCCGGGATTGCCGGAAATGACCGAC‑GGGCATTTGCCGCTTAACCCG‑a                                                                                                   >  2:140315/1‑238 (MQ=255)

                                                                                                                                       tGGACTTCCCCACCGGCCTGAAGTGGCAGGAGAAAACCCTGACCGTGAAACCGGCGTCGCGGAAAGGCCGGGTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑aa                                                                                                  <  1:10111/238‑1 (MQ=255)

                                                                                                                                        ggACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGCAAGATCTCTTTTCTCGCCCGGTATTTCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACa                                                                                                >  2:25130/1‑239 (MQ=255)

                                                                                                                                        ggACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCg                                                                                                                                                    >  2:1080776/1‑189 (MQ=255)

                                                                                                                                        ggACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCg                                                                                                                                                    <  1:1080776/189‑1 (MQ=255)

                                                                                                                                        ggACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGCATTGCCGGAAATGACCGAC‑GGGCATTTTCCGCTTAACCTG‑AACa                                                                                                >  2:10111/1‑239 (MQ=255)

                                                                                                                                            ttCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAg                                                                                                                                                                                                                                         <  1:40688/102‑1 (MQ=255)

                                                                                                                                             tcccccccGGGCTGAAGCGGGTGGAGAAACCCCTGACCCCGAACACGGCGGCGGTGAAAGGCCTGGTGATTGCCCGGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCCGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  1:482768/233‑1 (MQ=255)

                                                                                                                                             tcccccccGGCTCGAACTGGCAGGAGAAAGCCCTGACCATGAAACCGACGTCGCTGAAAGTCTTGCTGATTGCTCTGCCGAAA‑GTGTCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GACCCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTGTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCAGCTTAACCGG‑AACATTG‑aa                                                                                          <  1:887529/233‑1 (MQ=255)

                                                                                                                                             tcccccccGCCCTAAACTGGCAGGAGAAAACCCTGCCCCTGAAACCGGCGTCGCGGAAAGGCCTGCTGATTGCGCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  1:883974/233‑1 (MQ=255)

                                                                                                                                             tccccTCCGGCCTAAACTGGCAGGAAAAAACCCTGACCCGGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGGGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  1:873643/236‑1 (MQ=255)

                                                                                                                                             tCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  2:859739/239‑1 (MQ=255)

                                                                                                                                             tCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  2:534434/239‑1 (MQ=255)

                                                                                                                                             tCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  2:511256/239‑1 (MQ=255)

                                                                                                                                             tCACCTCCGGCCTGAACTGGCAGGAAAAAACCCTTCCCCTGAAGCCGGCGGTGTGGAAAGGCCTGGTGATTGCCCTGGCGAAA‑GTGGCGG‑AAGGGGCGCAGGAATAAGTGGTC‑TAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCCCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  1:989515/239‑1 (MQ=255)

                                                                                                                                             tCACCTCCGGCCTGAACGGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGATGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGTCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAAGGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑aa                                                                                          <  2:1210206/239‑1 (MQ=255)

                                                                                                                                              cACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGCAA‑GGTGCGG‑AAGGGGCGCAAGAAGAATTGGTC‑GCACCGAAAATTTAAAACCACCCGCCGGC‑TTAAAAACCGCGCGGCGCAAGCCTTAATGACCTCTTTTCTCCCCCGGAATTGACGGGAAAGAACGAC‑CTGCATTTGGCCCTTAAACTG‑acaagtgaaa                                                                                          >  1:834030/1‑230 (MQ=255)

                                                                                                                                                           aaCTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGGGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACCTTT‑AAGAGTTTAA‑GGGCGa                                                                           >  2:85029/1‑239 (MQ=255)

                                                                                                                                                            aCTGGCAGGATAAAACCCTGGCCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGag                                                                          <  2:894967/239‑1 (MQ=255)

                                                                                                                                                               ggCAGGGAAAACCCCTGACCCTGAAACCGACGTCGCGGAAAGGCCTGCTGATTGGTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAAACGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGagcag                                                                       <  1:106871/239‑1 (MQ=255)

                                                                                                                                                                     agaAAACCCTGACCCTGAAACCGACGTCGGTGAAAGGCCTGCTGAGTGCGCTGCCGAAA‑GGGGAGG‑AAGTGGCGAAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTgcg                                                                  <  1:290134/238‑1 (MQ=255)

                                                                                                                                                                             ctgaccctgaAACCGACGGCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACggg                                                         <  2:1290256/239‑1 (MQ=255)

                                                                                                                                                                              tgaccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGaaa                                                                                                                                                                          <  2:1031618/129‑1 (MQ=255)

                                                                                                                                                                               gaccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGCAAATTGAAAATCCCCAGCCGGA‑TGAAAAGCCCCTCGGCGAAAAGCTGAAAAATATCTTTTCTCGCCCGGTATTGCCGGGAATGACCGAC‑GTGCATTTGCCGCTTGACCTG‑GACCTTT‑GAAAGGTTAA‑GGGGGGGGACCTCGGCGGGACggggg                                                       >  1:1240471/1‑237 (MQ=255)

                                                                                                                                                                               gaccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCGGGTCGGCGAAGCGCTGAAAGATCTCTTTTCTTCCCCGGGATTGCCGGGAATAACCCACAGTACATTT‑CCCCGTAACCAG‑GAAATTGAAAGATTTAAA‑GGGcagcaacatgggcggaaggggg                                                        >  1:77409/1‑218 (MQ=255)

                                                                                                                                                                               gaccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑g                                                      >  2:1070111/1‑239 (MQ=255)

                                                                                                                                                                                  cctgaAACCGACGTCCGTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑Gac                                                    <  2:317579/238‑1 (MQ=255)

                                                                                                                                                                                  ccggaAACCGACGGCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑Gac                                                    <  2:1240471/235‑1 (MQ=255)

                                                                                                                                                                                   ctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑Gacac                                                  <  2:1095896/239‑1 (MQ=255)

                                                                                                                                                                                    tgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAACCGCTCGGCGAAACGCTGACAAATCTCCTTTCTCCCCCGGGATTGCCGGAAATAACCCAC‑GGGCATTTGCCGCTTAACCTG‑TACATTG‑AAGAGGTTAA‑AGGCGggccaccggcggggcagggccacccg                                                  >  1:837259/1‑216 (MQ=255)

                                                                                                                                                                                       aaCCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGGCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCTAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTGCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGa                                               <  2:939243/238‑1 (MQ=255)

                                                                                                                                                                                         ccGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACAt                                            <  2:261819/239‑1 (MQ=255)

                                                                                                                                                                                          cggcGTGGCTGAAAGGTCTGGTGATTGCTCGGCCGAAA‑GGGGCGG‑AAGTGGGGCAGGAAGAAGGGGTC‑GAACGAAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATc                                           <  1:576679/236‑1 (MQ=255)

                                                                                                                                                                                          cGACGTCGCTTAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATc                                           <  2:1301568/239‑1 (MQ=255)

                                                                                                                                                                                          cGACGTCGAGGAAAGGCCTGCTGGTTGTTCTGCCGAAA‑GTGGGGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATc                                           <  1:427427/239‑1 (MQ=255)

                                                                                                                                                                                            aCGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑CAGTGGCGCAGGAAGAAGTGGGC‑GAACCGAAAATTTAAAATCCCCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGGATTTCCGGAAATTACCGAC‑GGGACATTGCCCCTTAACCTGAAAAATTG‑AAG‑GTTTAAGGGGCGGACA‑CTGCCCGTG‑CGGGCGGCCACGGACATCAc                                         >  1:1313021/1‑239 (MQ=255)

                                                                                                                                                                                            aCGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCCGGAATAACCGCC‑GCGCATTTGCCGCGTAAACTG‑AACATTG‑AAAGGATTAA‑AGGCGAGCGGCTGCCCGGGACGGGG‑GACAAGGAAAacac                                         >  1:361225/1‑239 (MQ=255)

                                                                                                                                                                                              ggcgcgGAAAGGCCTGCTGATTGGTCGGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAGTTGAAAATCCGGAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGGTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCAcc                                        <  1:430968/236‑1 (MQ=255)

                                                                                                                                                                                               tcgcgtaaaGGCCTGCCAATTGCGCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCGCGGCGAAACGCTTAAAGATCTCTTTTCGCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGt                                      <  1:1116073/233‑1 (MQ=255)

                                                                                                                                                                                                          ccTGCTGGTTGCTCTGCCGAAA‑GGGGCGG‑AAGGGGGGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCAtg                            <  1:85029/238‑1 (MQ=255)

                                                                                                                                                                                                            tgctgATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCAtgct                          >  2:946529/1‑238 (MQ=255)

                                                                                                                                                                                                                   tGCCCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGaaa                   <  2:379600/238‑1 (MQ=255)

                                                                                                                                                                                                                       ctGCGGAAAGGTGGC‑G‑AAGTGGGGCAGGAAGAAGTGTGC‑GAACCGAAAAGTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GGGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGag              <  1:102441/239‑1 (MQ=255)

                                                                                                                                                                                                                       ctGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGag              <  2:1313021/239‑1 (MQ=255)

                                                                                                                                                                                                                        tGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGag                                                                          <  1:896873/179‑1 (MQ=255)

                                                                                                                                                                                                                        tGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGag                                                                          >  2:896873/1‑179 (MQ=255)

                                                                                                                                                                                                                         gCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGagca            <  2:862679/239‑1 (MQ=255)

                                                                                                                                                                                                                              aa‑GTGGCGG‑AGGGGGCGGAGGAAGAAGTGGAC‑GAACCGAAAATTGGAATGCCCCAGCCGGA‑TGAAAAGCCGCTCGGGGAAACGCTGAAAGGTCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCAtt       <  1:13182/239‑1 (MQ=255)

                                                                                                                                                                                                                              aa‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTTGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCAtt       <  2:503205/239‑1 (MQ=255)

                                                                                                                                                                                                                              aa‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTTGGC‑GGAACAAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGAGGAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGCACGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCAtt       <  1:664731/239‑1 (MQ=255)

                                                                                                                                                                                                                                     ggg‑AAGTGGGCCAGTAAAAAGTGGAC‑TAACCGAAAAGTGAAAATCCCCAGCCGGA‑TGAAAAGCCGCTCCGCGAAACGCTGAAAGACCTCTTTTCTCGCCCGGTATTGCCGGAAATGGCCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg  <  1:1104610/237‑1 (MQ=255)

                                                                                                                                                                                                                                     cGG‑AAGTGGCGAAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg  <  2:837259/238‑1 (MQ=255)

                                                                                                                                                                                                                                     cGG‑AAGGGGCGGAGGAAGAAGTGGTC‑GAAACGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg  <  2:377984/238‑1 (MQ=255)

                                                                                                                                                                                                                                      ggggaGTGGCGCAGGAAGAAGGGGGC‑GAAACGAAAAGTGAAAATCCGCAGCCGGG‑TGAAAAGCCGCTCGGCGAAACGGTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg  <  1:754747/234‑1 (MQ=255)

TGCCTCCTTCTGAACAGGTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAA‑GTGGCGG‑AAGTGGCGCAGGAAGAAGTGGTC‑GAACCGAAAATTGAAAATCCGCAGCCGGA‑TGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGAC‑GTGCATTTGCCGCTTAACCTG‑AACATTG‑AAGAGTTTAA‑GGGCGAGCAGCTGCGCGTGACGGGC‑GACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGG  >  NC_000913/4444441‑4444906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`