BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,463,875	T→G	11.3%	H279P (CAC→CCC)	treC ←	trehalose‑6‑P hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,463,875	0	T	G	11.3%	328.7 / 2.6	136	H279P (CAC→CCC)	treC	trehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand): ref base T (34/80); new base G (17/3); total (53/83)
Fisher's exact test for biased strand distribution p-value = 4.52e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCAC  >  NC_000913/4463638‑4464107

ggCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTTAAAGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTTACCACCGGGGTAATCGACCTTCAGGtg                                                                                                                                                                                                                                              >  2:46085/1‑239 (MQ=255)

 gCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTTTCCACCCGGATAAACGACCTTCAGGgga                                                                                                                                                                                                                                             >  2:1318023/1‑239 (MQ=255)

   cttCCCTGCCGTCCTTGCACCCCCATCCCCGACATTTTTGCCGCAGGCTCCGGGTATTCACCGTCACCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGTGATAATCGACCTTCAGGtgatg                                                                                                                                                                                                                                           <  1:1257769/238‑1 (MQ=255)

    ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGCGAAACAATGCGCGGCTGATCGTGGTTCCACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGACGGACACAATTTTTTAAACACC‑CCAAGACAGCTTGTGCCACCAGCCACTTTTTACCACCCGGAAAAAACACCTTCCGGagaag‑a                                                                                                                                                                                                                                         >  1:715135/1‑239 (MQ=255)

    ttCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCCGGTTTAGCCAGCGTCCATTTTTTACCCCCCGGGAAATTGACCTTCCGGcgatg‑a                                                                                                                                                                                                                                         >  2:1212928/1‑239 (MQ=255)

    gtCCATGCATGCCAGGCTGCACCATCGACAGCATTTTTTGCGCAGGCACGCGGTAGTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGGTCGTTGTTGCACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGtgatg                                                                                                                                                                                                                                           <  2:473595/237‑1 (MQ=255)

           catgccatgcCGCACCATCGACAGCATTTTTGCCGGAGGCCCGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATTCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTaa                                                                                                                                                                                                                                  <  2:1307935/239‑1 (MQ=255)

            atgccatgcAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTaa                                                                                                                                                                                                                                  <  2:960578/238‑1 (MQ=255)

              gccatgcAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCCCCGGGATAATCGACCTTCAGGGGATG‑AAAATTAAAgg                                                                                                                                                                                                                               >  2:1081680/1‑239 (MQ=255)

                    ccaacccATCGCCCGCATTTTTGCCGCAGGCCCCCGGTTATCCCCTTCAGCACCAAAGCTAGAAACAATGCGCGGCTGATCGGGGTTACACCAGAACAAGGCCTTCCATTCTACGTTGTGAATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCg                                                                                                                                                                                                                         <  1:1277903/234‑1 (MQ=255)

                     cgccccATCGCCAGCGTTGTTGGCGCAGGCACGCGGTATTCCCCTTCATCACCAAAGCGAGAAACAATGCGCTGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCCACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGa                                                                                                                                                                                                                        <  1:217891/235‑1 (MQ=255)

                       caCCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTCCGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACCCCACAAAGTCAGGGTTATCCACCGGCCCCTTTTTACCACCCGGGTAAAAGCCCTTAAAGGGAAG‑AAAAATaaaagacaccaaca                                                                                                                                                                                                                      >  1:737028/1‑228 (MQ=255)

                          cATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGGTTAGCCAGCGGCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATG‑GAAATTAAAGGTCATCgc                                                                                                                                                                                                                        >  2:731419/1‑233 (MQ=255)

                            tCGCCAGCATTTTTGCCGGAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCa                                                                                                                                                                                                                 <  2:469700/239‑1 (MQ=255)

                            tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑AAAATGTTTTTAAAGCCCCAAAGCACCGGTTAGCCTGCCTCCATTTTTTACCACCGCGGTAAAAGAACTTCAGGGGACGAAAAATTAAAgaacagcaaaaaaaa                                                                                                                                                                                                                  >  1:925717/1‑225 (MQ=255)

                            tCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCAGCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCa                                                                                                                                                                                                                 <  2:56897/239‑1 (MQ=255)

                               ccaccctttttGCCCGCGGCCCGCGGTATTCACCTTCAACACCAAAGCCGAAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTAAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTg                                                                                                                                                                                                              <  1:1128507/233‑1 (MQ=255)

                               ccAGCTTTTTTTCCGCAGGGCCGGGGTATTCACCTTCATCACCAAAGGGAGAAACAAGGCGCGGTTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTg                                                                                                                                                                                                              <  1:457425/239‑1 (MQ=255)

                                ccggttttttGCGGCGGGCTCGGGGTATTACCCTTCAGCAACAAAGCCAGAAACAAGGCGCGGCTAATCGGGGTTGCACCGGAACAAGGCATTCCATGGTACGTTGTGCATTCCGTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGTTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTg                                                                                                                                                                                                              <  1:457234/233‑1 (MQ=255)

                                    aTTTTTTCCCGAGGCACGCGGTATTCCCCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTGGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGt                                                                                                                                                                                                         <  2:432141/239‑1 (MQ=255)

                                        ttGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTTAACGCCCCCAAGTCAGGTTTAGCCAGCGTCCCTTTTTCTCCCCCGGGAAAAACGACACTCAAGTGAGG‑AAAAAAAAAAGGAACCGACAATTAACGGcccttccaa                                                                                                                                                                                                     >  1:639540/1‑232 (MQ=255)

                                         tgtccggagcccccgtgaTTCACCTTTACCACAGAAGGGAAAACCAAGGCGCGGATGATCGTGGTTACACCAGAACAAGGCATTCCAGGCTACGTTGTGCATGCCTTGTTGCCAGTGGCGGA‑ACAATGTTGTCAACGCCACAAAGTCAGGTTTAGCAAGGGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGAGG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCagag                                                                                                                                                                                                    <  1:1072714/222‑1 (MQ=255)

                                                          ttttcccTTCATCCCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGGTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCa                                                                                                                                                                                   <  1:1069814/233‑1 (MQ=255)

                                                          aTTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCa                                                                                                                                                                                   <  2:988651/238‑1 (MQ=255)

                                                               cctttacagcaaaaGCGAGAAAAAATGCGGGGCTGAGCGGGGTTACCCAAGAAAAAGGCATTCCCTGCTACGTTTTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTc                                                                                                                                                                            <  1:1238370/228‑1 (MQ=255)

                                                                   catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCCCAAAGTCAGGTTTAGCCAGAGGCCATTTTTTACCCACGGGATAATTCACCTTCAGGGGATG‑ACAATTAAAGGTCATCGTCAATTCACAGCCTGTCCGAG‑CCCCGTATCGCTGGCAATG‑GTCAAgg                                                                                                                                                                        >  2:1113884/1‑239 (MQ=255)

                                                                   catcaCCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCCACACCCGGATAAACGGCCTTCAGGGTATG‑GAAATTAAAGGTCATCGACAATTTACTGCCTGTTAGAG‑CCGCGTATACCTGGAAATG‑GTccaa                                                                                                                                                                         >  2:724101/1‑235 (MQ=255)

                                                                       cccaaacgagaaaaCCATGGCCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCtgg                                                                                                                                                                    <  2:1227164/228‑1 (MQ=255)

                                                                            agggagaACAAATGGGGGGGTGATCGTGGTTACACCAAAACAAGGCATTCCATGCTTCGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTggag                                                                                                                                                               <  1:1318023/236‑1 (MQ=255)

                                                                            aGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGTTG‑AAAATTAAAGGTCCTCGACCATTCACTGCCTGTCACAG‑CCGCGTTTCGCTGGCAATG‑CCCAAGGCcggtgggg                                                                                                                                                               >  2:426890/1‑237 (MQ=255)

                                                                                agaAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCCCAAAGTCAGGTTTTGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTTAGGGGATG‑AAAATTTAAGGTCATTGACAATTTACTGACTGTCAGAGACCGC‑TATTGCTGGCAAAG‑TCCAAAGCTGGGGGCGGACa                                                                                                                                                           >  2:512429/1‑239 (MQ=255)

                                                                                 gaAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGGCAAGTTTAGCCAACGTCCATTTTTCACCACCGGGGTAATCGACCTTCAGGAGATG‑AAAATTAAAGGTCATAGACAATTTCCTGCCTGTCATAG‑CCACGGATGGCTGGCAACG‑CTCCAGGCTGGTagaggcaca                                                                                                                                                          >  2:130450/1‑235 (MQ=255)

                                                                                  aaaCAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCGTTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACAt                                                                                                                                                          <  2:255417/238‑1 (MQ=255)

                                                                                  aaaCAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACAt                                                                                                                                                          <  2:768578/238‑1 (MQ=255)

                                                                                   aaCCATTCGCGGCTTATCGTTGTTAACCCAGAACAAGGCATTCCATGCTACGTTGGGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACAtt‑t                                                                                                                                                       <  2:536168/239‑1 (MQ=255)

                                                                                   aaCAATGCGCGGCTGATCGTGGTTACCCCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACAtt                                                                                                                                                         <  2:1014359/238‑1 (MQ=255)

                                                                                         tttttaaatgaaagggTTACCCCAGAAAAAGGACTTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTa                                                                                                                                                 <  1:465298/225‑1 (MQ=255)

                                                                                                       ggTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑AAAATGTTTTCAACGCCACAAAGGCAGGGTTAGCCCGCGTCCATTTTTTTCCACCGGGATAAACGACCTTCAGGGGATG‑AAAATTAAAGGCCATCGACAAATCAATGCCCGTCAAAG‑CCCCGCATCGCTGGCACTG‑TTCAAGGCTGGTGGGGGGCATT‑TTCCCACCGGCAATTAAcccc                                                                                                                                   >  1:36966/1‑238 (MQ=255)

                                                                                                         ttACACCGGAACAAGGAATTCCATGATACGTTGTGCTTTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAAGCGCACAATGTCAGGTATAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCcgc                                                                                                                                  <  1:862020/238‑1 (MQ=255)

                                                                                                         ttACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTTCCCACCGGGATAATAGACCTTCAGGGGATG‑AAAATTGAAGGGCATTGACAATTCACTGCCTGTCAGAG‑CCCCGTATCGCTGGCAAGT‑GTCAAGGCCGGGGGATGGCATT‑TTACCTACGGTCCTTAACCcgcg                                                                                                                                 >  2:313421/1‑239 (MQ=255)

                                                                                                         ttACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑AAAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACACTTCACTGCCTGTCAGAG‑CCGCCTATCGCTTGCAATG‑CTCAAGGCTGGTGGGGGACCTT‑TCACCTCAGGCCCTTAACCcgcg                                                                                                                                 >  2:709657/1‑239 (MQ=255)

                                                                                                          tcccccaaatcAAGGCCTTCCATTCTTCGGTGTGCATTCCTTTTTGCCTGTGGCGGA‑ACAATTTTTTCAACGCCACAAAGTCAGGTTTTGCCAGCGTCCATTTTTCACCACCGGGATAATCGCCCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGt                                                                                                                                <  1:1081680/229‑1 (MQ=255)

                                                                                                          tACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGt                                                                                                                                <  2:634140/239‑1 (MQ=255)

                                                                                                          tACACCAGAACAAGGCATTCCATCCTACGTTGTGCATTCCTTGTTGCCAGTTGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGt                                                                                                                                <  2:1149560/239‑1 (MQ=255)

                                                                                                            cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTTACCACCGGGATAAACGACCTTCAGGGGATG‑AAAATTTAAGGTCATCACCAATTCCCTGCCCGTCAGAA‑CCCCGGATACCTGGGAATGCCCCAAGCCTGG‑GGGGGGCAAT‑TCAccacacggcattaaacccccggg                                                                                                                              >  1:500110/1‑218 (MQ=255)

                                                                                                            cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAAGTCATCGACAATTCACTGCCTGTCAGAG‑CCCCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGGCATT‑TCACCTACGGTCATTAACCCGCGTgg                                                                                                                              >  2:287866/1‑239 (MQ=255)

                                                                                                            cacCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAACCCGCGTCCATTTTTCACCACCGGGGTAATCGACCTTCAGGTGATG‑AAAATTCAAGGGCATCGACAATTCACTGCCTGTCACAG‑CCGCGTATCGCTGGCCATG‑CTCAAGGCTGGTGGCGGCCATT‑TCACCTACGGGCATTAACCCGCGTgg                                                                                                                              >  2:958290/1‑239 (MQ=255)

                                                                                                             acCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTc                                                                                                                                                                            <  2:970189/193‑1 (MQ=255)

                                                                                                               cAGAACAAGGCATTCCATGCTTCGTTGTGCATTCCTGGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGt                                                                                                                           <  2:719776/239‑1 (MQ=255)

                                                                                                                  aaCAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGGATCGCTGGCAATG‑TTCAAGGCTGGGGGGGGACATT‑TAACCTATGGTCCTTAAcctgtctcttataaa                                                                                                                        >  2:465298/1‑226 (MQ=255)

                                                                                                                  aaCAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACAACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAAAG‑CCGCGTATCCCTGGCAATG‑CTCAAGGCTGGGGGAGGAAATT‑TCACCTACGGTCATTAACCCGCGTGGCGTaaa                                                                                                                        >  2:796350/1‑239 (MQ=255)

                                                                                                                       ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCCATG‑CTCAAGGCTGGAGGAGGCGATT‑TCACCTCAGGTCCTTAACCCGCGAGGCGGAAAaaca                                                                                                                    >  2:1278338/1‑238 (MQ=255)

                                                                                                                       ggCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGGTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCCGGTGATG‑CAAATTAAAGGTCATCGACAACTCAATGCCTTGCAGAG‑CCCCGGATCCCTGGACATG‑CTCAAGGCTGGTGGAGGGCATT‑TTACCTACGGTCCTTAAACCCACTGGCGTaaaaaaa                                                                                                                    >  2:233981/1‑234 (MQ=255)

                                                                                                                         ctttCCATGCGACGTTGTGCGTTTCCTTTTGCCAGTTGCTGA‑ACAATGTTTTCAAGGCCCCAAAGTCCGGTTGAGCCAGCGTCCATTTTCCACCCCCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCGCTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATc                                                                                                                  <  1:750207/236‑1 (MQ=255)

                                                                                                                         cATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGGCATCGACAATTCACTGCCTGTCAGAG‑CCCCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGGCATTAAACCCCAGGGGGGAAAcacaac                                                                                                                  >  2:115540/1‑236 (MQ=255)

                                                                                                                          ttttcctttctcGTTGTCCTTTTCTTTTTGGCGGGGGCGAA‑ATAATGTTTTCAACGCCACAAAGTCAGGTGTAGCAAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATcgc                                                                                                                <  1:130611/228‑1 (MQ=255)

                                                                                                                            tCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTTAACGCCACAAAGTCCGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGGGATG‑AAAATTAAAAGGCATAGACAATTAACTGCCCGTCAAAG‑CCCCGTATCGCGTGCAAAG‑CTCAAGGCTGGTGGGGGGCACT‑TTACCAACGGTGCTTAACCCCCGAgggcgaaaaaaatgaggg                                                                                                              >  1:1166248/1‑223 (MQ=255)

                                                                                                                                 tcttcGTTGTTCATTCCTGGTTGCCAGTGGCTGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTTCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCAt                                                                                                         <  1:792564/235‑1 (MQ=255)

                                                                                                                                          tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCCCAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAAGTGATG‑AAAATTAAAGGTCATCGGCAATTCACTGCCTGTCAGAG‑CCGCGAATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGGAAAAAC‑‑CCCGGTTCCTTTCCTGCaaa                                                                                               >  2:334256/1‑238 (MQ=255)

                                                                                                                                          tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAAACGACCTTCACGTGATG‑AAAATTAAAATGCACCGACAATTCACTCCCTGTCACAG‑CCCGGTACCCCTTGCAATT‑CGCCAAGCTGGGGGAGGCAATTCTACCCCACGG‑CCTTAACCACCGGGGCGGAAAcacaaccccgggcactccgggcaa                                                                                                >  1:498612/1‑219 (MQ=255)

                                                                                                                                          tgCATTCCTTGTTGCCAGTGGCGGA‑ACAATGGTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGGTAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTTGCAGAG‑CCGCGTATCGCTGGCAATG‑CCCAAGGCTGGGGGAGGGCCTT‑TAAACAACGGCCATTAACCCCCGTGGCGTAAAAACCACCCGGTCCATCGTGTGcca                                                                                                >  2:1029580/1‑237 (MQ=255)

                                                                                                                                                   tgttgCCAGGGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCgtg                                                                                       <  2:1095570/239‑1 (MQ=255)

                                                                                                                                                    gttgCCAGTGGCGGA‑ACAATGTTTTCAAAGCCACAAAGTCAGGTTTAGCCAACGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGCATCGCTGGCCATG‑CTCAAACCTGGGGGAGGGCATT‑TCACCTCCGGGCATTCAACCGCGTGGCGTGAACCAACACCGGTTCATTTCGTggcacaacactgtg                                                                                      >  2:1055173/1‑226 (MQ=255)

                                                                                                                                                           gTGGCGGA‑ACTATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTAc                                                                                                                                                <  1:301132/174‑1 (MQ=255)

                                                                                                                                                           gTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTAc                                                                                                                                                >  2:301132/1‑174 (MQ=255)

                                                                                                                                                                  gtaCAATTTTTTCCACTCCACAAAGTCAGGTTTAGGCAGCGCCCCTTTTGCACCACCGAGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACTATTCACTGCCTGGCAGAG‑CCGCGTATCGCTGGGATTG‑CGCAAGGCTGGTGGAGGACATT‑TCACCTACTGTCCTTAACCCGCGTGCCGTAAACGCATCGCTGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  1:784221/237‑1 (MQ=255)

                                                                                                                                                                  a‑aCAATTGTTTAAACGCCATGAAGTCAGGTTTAGCCAGCGTCCATTTTCCACCCCCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:842298/238‑1 (MQ=255)

                                                                                                                                                                  a‑aCAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCCCCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:500110/238‑1 (MQ=255)

                                                                                                                                                                  a‑aCAATGTTTTCAACGCCACAAAGGCAGGTTTAGCCAGCGTCCATTTTGCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:366506/238‑1 (MQ=255)

                                                                                                                                                                  a‑aCAATGTTTTCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:36966/238‑1 (MQ=255)

                                                                                                                                                                  a‑aCAATGTTTTCAACGCCAAAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:908586/238‑1 (MQ=255)

                                                                                                                                                                  a‑aCAATGTTTTCAACGCCAAAAAGGCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:585245/238‑1 (MQ=255)

                                                                                                                                                                  a‑aCAATGTTGTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:740946/238‑1 (MQ=255)

                                                                                                                                                                   atcattcttttCAACTCCAAGAATGTAGGTTTAGCCAGCGGCAATTTTTCACCACCGGGATAATCGACCTTCAGGGGATG‑AAAAGTGAAGGTCATCGAAAATTCACTGCCTGGCAGAG‑CCCGGTATCGCTGGCAATG‑CTCAAGGCTGGTAGAGGACATT‑TCACCTACGGTCATTAACCCGCGTTGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  1:2673/231‑1 (MQ=255)

                                                                                                                                                                   aatattttttttaagcccACAAAGTAAGGTTTAGCCTGGTACCATTTTTAACCTCCGGGATAATCGACCTTCAGGTGATG‑AAAATGAAAGGTCAGCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  1:70670/222‑1 (MQ=255)

                                                                                                                                                                   aaaaatttttctaatgCCAAAAAGTCAGGTTTAGCCAGGGTCCATTTTTCCCCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCCCTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:849642/223‑1 (MQ=255)

                                                                                                                                                                   aaaaaTGGTTTCAACGCCACAAAGTCAGGTTTAGCCCGCGTCCATTTGTCCCCAACGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGt                                                                         <  2:734732/235‑1 (MQ=255)

                                                                                                                                                                             tCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCCCGGTTCATCTCGTGCAAAAACCCGGGTGCTCGGGGCCCATCGGTGTAGaac                                                              >  1:840793/1‑238 (MQ=255)

                                                                                                                                                                                  gCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCAATGCCTGTCAGAG‑CCGCGAATCGCTGGCAATG‑CTCACGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGGAAACACATCCCGGTTCATCTCGCGCAAAAACTCGCGTGCCCGTGGACCGTCGGGGAAGAAACGAc                                                          >  1:969029/1‑238 (MQ=255)

                                                                                                                                                                                    cacaAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTAc                                                                                                                                                <  2:828221/150‑1 (MQ=255)

                                                                                                                                                                                         atccgggttgtgCCAGGGTCAATTTTTCCACCGCGGGTAAATGGCCCTTCGGGTGGTG‑AAAATTAAAGGGCATCGGAAAGTCGATGCCTGTCAGAG‑CCGCGTATCGCTGTCAATG‑CTCAAGGGTGGTTGAGGACATT‑TCACCTCCGGTCATTACCCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCACGTCGGTGTAGAAGCGACGCCCGTCg                                                  <  1:1025863/228‑1 (MQ=255)

                                                                                                                                                                                         agttatgTTTTGCCAGCGTCCTTTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCAGCGACAATTCACTGCAGGTCAGAG‑CCGCGGATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGTGACGCCCGTCg                                                  <  1:285049/233‑1 (MQ=255)

                                                                                                                                                                                         aGTCAGGTTTAGCCAGGGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                  <  2:847584/239‑1 (MQ=255)

                                                                                                                                                                                         aGTAAGGTTTAGCCAGCGTCCATTTTTCGCCGCCGGGATAATCGACCTGCAGGTGATG‑AAAATTAAAGGGCATCGACAATTCGCTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                  <  2:1223891/239‑1 (MQ=255)

                                                                                                                                                                                                 ttAGCCGGTGTCCATTTTTCCCACCCGGGATACTCGGACCTCAGGTGATG‑AAAATTAAAGGTCATGGACAATTCACTGCCTGTCAGGG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACCTT‑TCCCCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAg                                         <  1:1212928/239‑1 (MQ=255)

                                                                                                                                                                                                 ttAGCCAGCGTCCATTTTTCCCCACCGGGATAATCTACCGTCAGGTTATG‑AAAAGTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTGAAGGCTGGTGGAGGACATT‑TCACCTACGGGCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAg                                         <  1:247516/239‑1 (MQ=255)

                                                                                                                                                                                                  gtgcacgCGTCCATTTTTCCCCCCCGGGATAACCGGCCTTCAGGTGAAG‑AAAATTAAAGGGCATCGACAAGTCACTGCCTGTCAGAG‑GCGCGTATCGCTGGCAATG‑CTCAAGGCTGGAGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAg                                         <  1:362445/232‑1 (MQ=255)

                                                                                                                                                                                                         cGTCCATTTTTCACCACCGGGATAATCGTCCTTCAGGTGATG‑AAAATTAAAGGACATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCGGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATGGCGGTTCATCTCTGGCAAAACCTCGTGTGCTCGTGGc                                                                             >  2:707448/1‑196 (MQ=255)

                                                                                                                                                                                                         cGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAGACCATCGCGGTTATCCTCGGGCAAAAACTCGAGTGCTCGTGGCCCGTCGGGGGAGAAGAGACGCCCGGCGCCGTACCAGGT‑TTCAg                                 >  1:321551/1‑239 (MQ=255)

                                                                                                                                                                                                         cGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGGCTTCAg                                 >  2:247516/1‑239 (MQ=255)

                                                                                                                                                                                                         cGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGGGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAg                                 >  2:362445/1‑239 (MQ=255)

                                                                                                                                                                                                                ttttCCCCCCCGGGATAACCGGCTTTCAGGTGATG‑AAAATTAAAGGTCACCGACAATTCACTGGCTGTCAGGG‑CCGCGTATCGCTGGCAATG‑CCCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAAcg                          <  1:468357/239‑1 (MQ=255)

                                                                                                                                                                                                                         ccGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGGGGAGGACATT‑TCACCTACGGTCATTAACCCGCGCGGCGTAAACCCATCCCGGTTCCTCTCGTGCAAAAACTCGGTTCCTCGTGGCCCGCCGGGTTAGAAGCGACCCCCACCCCCGT‑CCAGAGCTTCCGGGAAACACggggggtt                 >  1:207459/1‑234 (MQ=255)

                                                                                                                                                                                                                          cGGGATAATCGCCCTTTAGGTTATG‑AAAATTAAAGTTCACCTACAATTCCCTGCCTGTCAGAG‑CCGCGTGTCGCTGGAAAGG‑CGCAAGGCGGGTGGAGGCCATT‑TCACCTCCGGTCATTAACCCGCGTGGCGTAACCCCATCGCGGTTCATCTCGTGCAAAAACCCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAACGCGGGTCTTTg                <  1:413168/239‑1 (MQ=255)

                                                                                                                                                                                                                          cGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                  >  2:879998/1‑206 (MQ=255)

                                                                                                                                                                                                                          cGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCg                                                  <  1:879998/206‑1 (MQ=255)

                                                                                                                                                                                                                                   cggccTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCCCTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGa       <  2:769272/236‑1 (MQ=255)

                                                                                                                                                                                                                                   cggccTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGa       <  2:639540/236‑1 (MQ=255)

                                                                                                                                                                                                                                      ccTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCCCTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGATTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGAtt     <  2:657505/238‑1 (MQ=255)

                                                                                                                                                                                                                                        gtCAGGGTATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGGGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTcac  <  2:1071734/238‑1 (MQ=255)

GGCGTTCCCTGCATGCCATGCAGCACCATCGCCAGCATTTTTGCCGCAGGCACGCGGTATTCACCTTCATCACCAAAGCGAGAAACAATGCGCGGCTGATCGTGGTTACACCAGAACAAGGCATTCCATGCTACGTTGTGCATTCCTTGTTGCCAGTGGCGGA‑ACAATGTTTTCAACGCCACAAAGTCAGGTTTAGCCAGCGTCCATTTTTCACCACCGGGATAATCGACCTTCAGGTGATG‑AAAATTAAAGGTCATCGACAATTCACTGCCTGTCAGAG‑CCGCGTATCGCTGGCAATG‑CTCAAGGCTGGTGGAGGACATT‑TCACCTACGGTCATTAACCCGCGTGGCGTAAACACATCGCGGTTCATCTCGTGCAAAAACTCGTGTGCTCGTGGCCCGTCGGTGTAGAAGCGACGCCCGTCGCCGT‑CCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCAC  >  NC_000913/4463638‑4464107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`