BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,588,080	A→C	16.0%	W263G (TGG→GGG)	yjiA ←	metal‑binding GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,588,080	0	A	C	16.0%	234.9 / 9.2	107	W263G (TGG→GGG)	yjiA	metal‑binding GTPase
Reads supporting (aligned to +/- strand): ref base A (35/52); new base C (11/9); total (46/61)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TGGATATGGACTATTTTGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGA  >  NC_000913/4587842‑4588313

tGGATTTGGCATCTTTTGAGTTAAATTCCAGGCGTGGTGCGCGTGCCCCTTTCCCGGCCCAGATCCCCCTACTTCGTCAACCCCGCAAACTCAGCCCGAATCTCTGCCTCCGGCAGCTGGATCCCGATAAACACCAGCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCc‑a                                                                                                                                                                                                                                             <  1:446566/239‑1 (MQ=255)

     ttGGACTATTTTGAGTGAAATTTCAGGCTGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTGTTCATCGCCCCACGGCCTGTCTCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCa                                                                                                                                                                                                                                        <  1:492707/238‑1 (MQ=255)

         aCTATTTTGAGTGAAATATCAGGCCGGGTGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCCCCGGCAGCTGGATCCCCATAAACAACATCGTGCTATGCGGTTTTTCCTCGCCCCACGGCCTGTCCCAGTCCGCGCTGTAGAGGGCGTTGACCCCCTGCAACACCACGCGGTACGGTTCGCCGTACATCc‑ccaacccg                                                                                                                                                                                                                                      >  1:85855/1‑232 (MQ=255)

           ttttttGAGTGAAATTTCAGGCCGGAGGGGCGTGCGCCTTATCCCGCCCATAACCCCTTACTTCCGCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCtt                                                                                                                                                                                                                                  <  1:865804/237‑1 (MQ=255)

            aTTTTGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGCACAGCAGGCGGTTAGGTTCGCCGCCAATCC‑ACACCATCCCttt                                                                                                                                                                                                                                 >  2:1202270/1‑239 (MQ=255)

             ttttGAGTGAAATATCAGGCCGGATGCGGCTTCGCCTTATCCGGCCCATAACCCCTTACCTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTg                                                                                                                                                                                                                                <  2:570446/239‑1 (MQ=255)

             ttttGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTg                                                                                                                                                                                                                                <  2:823159/239‑1 (MQ=255)

             ttttGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCCGCGTCAATCC‑CAAACATCCCtttt                                                                                                                                                                                                                                >  2:1177032/1‑238 (MQ=255)

             ttttGAATGAAATTCCCGGCCGGATGCCGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTg                                                                                                                                                                                                                                <  2:449252/239‑1 (MQ=255)

                  aGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGACATGTCCCAGTCGGCGCTGTACAGGCGCTGGACGCCCTGGCACAGCCGGCGGTTTGGTTCGCCGTCAATCC‑CCCTCATCCCtctttac                                                                                                                                                                                                                             >  2:700298/1‑231 (MQ=255)

                  aGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCCTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGCCGCCCTGGAACAGCAGCGGGCTAGGGTCGGCGGCAATCC‑ACAGCAACCCTTTTGAACg                                                                                                                                                                                                                           >  1:1327207/1‑239 (MQ=255)

                    ttaaaTACCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGt                                                                                                                                                                                                                          <  2:1257876/236‑1 (MQ=255)

                       aaTATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCa                                                                                                                                                                                                                      >  2:682857/1‑239 (MQ=255)

                                ccGGATGCGGTCGCCCCTTATCCGGCCCATAACCCCTTCCTTCCCCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACTCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGATGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTAt                                                                                                                                                                                                                <  2:784108/236‑1 (MQ=255)

                                   gATGCGGCTGCTCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGCTCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCCGTCCCAGTCGGCGCTGTAGAGGGGCTGGACGCCCTGGGACAGCAGGCGGTTAGGTTCGCCGTCAATAC‑ACACCACCCCCTTggacagcaaccagtgttacggca                                                                                                                                                                                                          >  1:1038295/1‑217 (MQ=255)

                                   gATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGTCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGGTGGGTTCGCCGTCAATCC‑CCAGCATACCTTTGTAAAGCAGCAGgtttttccccg                                                                                                                                                                                                          >  2:609769/1‑231 (MQ=255)

                                         gCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACACCAGGCGGTTAGGTTCGCCGGCAACCC‑CCACACGTCCTTTGTAACCTACCAGTTTATCCCCCCGCTcc                                                                                                                                                                                                     >  2:159158/1‑238 (MQ=255)

                                             ccccTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACGCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCcagcag                                                                                                                                                                                                <  2:1149240/237‑1 (MQ=255)

                                                   acccGGCCCGAAACCCCTTATTTCCTCAACCCCGCAAACGAAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGGTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGtt                                                                                                                                                                                          <  2:358820/237‑1 (MQ=255)

                                                         cccATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCCGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGACGGCGGTTAGGTCCGCCGGACATCC‑CCAGAATCCCTTTGTAACGTAGCAGGTTATCCGCCGAcacacaaaaacagttttcca                                                                                                                                                                                     >  1:633982/1‑219 (MQ=255)

                                                         cccATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGGTTTCCa                                                                                                                                                                                     >  2:385854/1‑238 (MQ=255)

                                                                   cTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCACCACGTCCTGTCCCATTCGGCGCTGTCGAGGCGCTTGACGCCCTGGAACAGCAGCCGGGTCGGTTCGCAGCCACTAC‑CCACAAACCCTTTCTCAAGGAGGAGTTTATCCGCCGCCTCCCACAGCGGGTTTTctcaccatcgggca                                                                                                                                                                          >  1:525456/1‑226 (MQ=255)

                                                                   cTTACTCCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTTTAACGCAGCAGTTTATCCGCCGACTCCACCAGCAGGtctgtcccttttacaca                                                                                                                                                                           >  2:868076/1‑222 (MQ=255)

                                                                      ctcccccgacccccccAAACGCCACCTGGATATCGTCCGCCGGCAGCGGGACCCCGAGAAACCGCATCGGGCTTTGCGTTTGTGCACCGCCGCACGGCCGGTCCCAGGCGGCGCTGTAGAGCCGCTGGACACCCTGGATCAGCAGGCGGTTAGGGTCGCCGCCAATCC‑CCAGCATCGCTTTGTAAAGTAGCAGTTGATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACt                                                                                                                                                                       <  1:235783/229‑1 (MQ=255)

                                                                      aCTTTCTCAACCCCGCAAACGCAGCCCGCATCTCTTCCTCCCGCAGCTGGATCCCGATAAACACCATCGTGCTAGCCGGTTTTTCATCACCCCACGGCCCGACCCGGTCGGCGCTGTAGCGGCGCTCGTCGCCCTACAACCGCAGGCGGTTGGCTCTGCCCTAACTCC‑ACAGAGTCCCTTTGTAACGTCGCCGTGTATCCGCGGACTCCACCAGCAGGTTTTCCCTCCCCCGGGAAAca                                                                                                                                                                       >  1:1319067/1‑238 (MQ=255)

                                                                      aCTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCGTCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑CCAGCATCCCTTTGTAACGTAGCAGGTTTTCCGCCGACTCCAGCAGCAGGGTTTCCATCCCCCGGGAAACt                                                                                                                                                                       >  2:1058723/1‑239 (MQ=255)

                                                                         tcctccCCCCCGCAAACCCCCCCCGAATCTCTTCCTCCGGACGCTGGAGCCCGATAACCCCCATCGGGCTATCGGGTTTTTATTCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCg                                                                                                                                                                    <  1:917362/239‑1 (MQ=255)

                                                                         tCCCCACCCCCGCAAACCCCGCCCGAATTTTTGCCTCCGGCAGCTGGACCCCGATAAACCCCAGCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGGCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCg                                                                                                                                                                    <  1:206713/239‑1 (MQ=255)

                                                                           cTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCACCCGGTTTTCCATCACACGGGCAACttt                                                                                                                                                                     >  2:76452/1‑235 (MQ=255)

                                                                           cTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATACGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTTGGTTGGCCGCCGATCC‑ACCTCACCCCTTTGTAACTCAGCAGTTTTTCCCCCCACCCCCCCACCAGGATTTCCATCCACCGGGAAAcacc                                                                                                                                                                     >  1:486782/1‑233 (MQ=255)

                                                                              aaCCCCGCAAACGCAGCCCGAATCTCTTCCCCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGGCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑at                                                                                                                                                              <  2:169702/239‑1 (MQ=255)

                                                                                ccccGAAAACGCAGCCCGAATCTCTGCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCTCGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑atat                                                                                                                                                            <  2:521162/239‑1 (MQ=255)

                                                                                   cGCAAACGCAGCCCGAATCTCTTCCGCCGGCAGCTGGATCCCGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTa                                                                                                                                                         <  2:703850/239‑1 (MQ=255)

                                                                                              cccGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTaa                                                                                                                                                 <  2:1015128/236‑1 (MQ=255)

                                                                                               ccctaTCTCTTCCCCCGGGAGCCCGACCCCGATAAACCCCCGCGTGCTATGCGGTTTTTCCTCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAAt                                                                                                                                                <  1:910513/232‑1 (MQ=255)

                                                                                                 gAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGt                                                                                                                                           <  2:399812/239‑1 (MQ=255)

                                                                                                 cactctctcCCTCCGGCCCCCGCACCCCGATAAACCCCTGGGTGCTTTGCGGTTTTTCCTCGCCCCGCGGCCTGGCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGACAAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGt                                                                                                                                           <  1:658732/236‑1 (MQ=255)

                                                                                                   actctttcccccgccacgcgggtCCCGATAACCCACACTGGGCTATGGGGTTTTTCCTCGCCCCACGGCCTGCCCCAGGCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTc                                                                                                                                         <  1:682857/217‑1 (MQ=255)

                                                                                                        ttCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAAAGTGGCGGTTTTTCCGCCGCGCCCAGCAGCAGGGTTTTCTTCAACCGGGGAACTTCCCTG‑TCACCTACCCGGGAATCCACTTccaccc                                                                                                                                    >  1:1015128/1‑238 (MQ=255)

                                                                                                               gggcggtGGAACCCGATAAACCCCATCGGGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCCGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATcga                                                                                                                             <  2:471434/233‑1 (MQ=255)

                                                                                                               ggCAGCTGGATCCCGAAAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATcga                                                                                                                             <  2:1327207/239‑1 (MQ=255)

                                                                                                                gCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATACCTTTGTAACGTAGAGGTTTATCCGCCGCCCCCCCAAGCAGGTTTTCCATCACGCGGGGAACTTCGCTG‑ATTTCTACCGGGTAATCCACTTCACCcacacacaag                                                                                                                            >  1:921029/1‑233 (MQ=255)

                                                                                                                gCAGCTGGATCCCGATAAACACCATCGTGCTAAGCGGTTTTTTATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGATGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATcgac                                                                                                                            <  2:85855/239‑1 (MQ=255)

                                                                                                                        aTCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAatat                                                                                                                     <  2:101195/238‑1 (MQ=255)

                                                                                                                           ccGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGtt                                                                                                                 <  2:481557/239‑1 (MQ=255)

                                                                                                                              aTAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGGTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCCCAAACGACGAAACATCGTTTTg                                                                                                              >  2:105160/1‑239 (MQ=255)

                                                                                                                                  acacCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCATTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCTGTACGGTTCGCGGTCAATCC‑ACAGCATCCCTTTGTAAAGTAGCGGTTTATCCACCGACTCAAGCAGCAGGTTTTCCATCACGCGGGCAACTTCTCTT‑ATATCTACCCGGTAAACCATTTCCACCCAAAACGACATAACATCGGTTTGTTTa                                                                                                          >  1:365502/1‑239 (MQ=255)

                                                                                                                                   ccccgtctgggtATGCGGTTTTTCAGCGCCCACCGGGCTGTCCCGGGCGGCGCTGGAGAGGCGCAGGACGCCCTGGAAAAGAGGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTGGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTAt                                                                                                         <  1:963108/228‑1 (MQ=255)

                                                                                                                                   ccccATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATGTCGTTTTGTTTAt                                                                                                         <  2:633982/237‑1 (MQ=255)

                                                                                                                                    cgcctCGTGCTATGGGGTTTTTCGGCGCCCCACGGACTGTCCCGGGCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACGGCAGGCGGTTAGGTTCGCCGTCAAACC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATc                                                                                                        <  1:1058723/235‑1 (MQ=255)

                                                                                                                                         cGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTCGGGTCGCCGTCACACC‑ACAGCATCCCTTTGTAACGTCGCAGTTTATCCGCCGACTCCAGCAGCAGGGTTTCCCTCACGCGGGAAACTTCGCTG‑CTATCTCCCGGGTAATCCAGTTCCACCCCCATCGACGAAAAATCGTTTTGTTTATATGCGa                                                                                                   >  1:239728/1‑239 (MQ=255)

                                                                                                                                         cGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGCTTCGCCGTCAATCC‑ACAGCATCCCCTTTTAACGTAGCAGTTTATACGCCGAATCCAGCAGCAGGATTTCCCTCCAGCGGGAAACCTCGCTGCATA‑CTACCGGGTAATCCCGTTACCCCACAAACCAAGaaaacacatctttattatctgcca                                                                                                   >  1:138838/1‑218 (MQ=255)

                                                                                                                                           tGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGGTTGCCGCCAATCC‑CCCGCATCCCTTTTTAACGGAGCAGTTTTTCCGCCGACTCCAGCAGCAGGGTTTCCATCCAGAGGGAAACTTCCCGG‑AAATTTCCCGGGTACTCACATTCCACCACACTCCAGTAAAAAACGtttttttttatatcgcaa                                                                                                 >  1:741409/1‑229 (MQ=255)

                                                                                                                                            gCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATa                                                                                                 <  2:350974/238‑1 (MQ=255)

                                                                                                                                                tGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAAAGTAGCAGTTTTTCCGCCGGCTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCGG‑ATATCTCCCGGGGAATCCAAGTCCACCAAAATCAAACAAAAAACCTTTTGTTTATCttgggaacagtg                                                                                            >  1:1151032/1‑228 (MQ=255)

                                                                                                                                                      tcttcagtgCCCCGCGGCCTGTGCCATGCGTCGCTGTAGGGGCGCTGGACGCCCTGGAACAGCAGGGGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTACCCGCCGACGTCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAAcg                                                                                     <  2:327769/231‑1 (MQ=255)

                                                                                                                                                               ccccACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑CCAGCATCCCCTTGTAACGTAGCAGTTTTTCCGCCGACTCCCACAACAGGGTTTCCCTTAGGCGGGGAAACTCGCCG‑ATATCGACCGGGCAATGCCGATCCACACCAATGGCACAAATAACGTCTTTGTTATATTCGATTAATGGG‑AAACGCAGttgtgt                                                                            >  1:1201078/1‑235 (MQ=255)

                                                                                                                                                               ccccACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGt                                                                            >  2:399091/1‑239 (MQ=255)

                                                                                                                                                                          gTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCCCGCGGGAAACTTCGCGG‑AAATCTACCGGGTAATCCAGTTCCACCCCAATCCACGAAAAAACGTTTTGTTTTTTTTCACTAAAATGGAAAACGGGGTTTgggcggcgagcgt                                                                  >  1:1094505/1‑228 (MQ=255)

                                                                                                                                                                          gTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGGAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGTTAAAGTGG‑AAAACCGGTTTGGGGGGGACGACGt                                                                 >  2:1028361/1‑239 (MQ=255)

                                                                                                                                                                               aGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCCATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGCAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCCAAATCGACGAAATAACGGTTTGTTTATCTGCAGTAAAGTGG‑AAACCGGGTTTGGGGCTGACGCCGTTttct                                                            >  2:144877/1‑239 (MQ=255)

                                                                                                                                                                                 tCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTAACGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGCTAAAATGG‑AAACGCGGGTTGGTGCTGACGACGTtttttt                                                           >  2:1146514/1‑238 (MQ=255)

                                                                                                                                                                                     cgcTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑CCAGCATCCCTTTGTAACGTAGCCGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAACCTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGCCGAAATATCGTTTTGTTTATCTGCGCTAAATGGG‑CAACGCGGTTTGGTGCTGCCGACGTTTTTTTCCAGc                                                      >  2:189204/1‑239 (MQ=255)

                                                                                                                                                                                     cgcTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTTCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGGTTTTTTCCAGc                                                      >  2:87987/1‑239 (MQ=255)

                                                                                                                                                                                     cgcTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCCCAATCGACGGAAAATCGTTTTGTTTATCTGCGGTAAAGTGG‑AAACGCGGGTTTGGGCTGACGACGTTTTTTTCCCGc                                                      >  2:311324/1‑239 (MQ=255)

                                                                                                                                                                                        tGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGtt                                                                                                                 <  2:912304/178‑1 (MQ=255)

                                                                                                                                                                                          tAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATaaa                                                 <  2:1151032/239‑1 (MQ=255)

                                                                                                                                                                                               gcgcTGGGCGCCCTGGAACAGCAGCCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCGAGCAGCAGGTTTTCCATCACGCTGGAAACTTCGCTG‑ATATCTACCGGGTAAGCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  2:595306/239‑1 (MQ=255)

                                                                                                                                                                                                cgcgtgACGCCCGTGAACAGCAGGCGGTTTGGTTCGCCGGCAATCC‑ACAGCATCCCGTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  2:525456/233‑1 (MQ=255)

                                                                                                                                                                                                cgcTGGCCGCCTGGGAAAAGCAGGTGGTTTGGTTCGCCCTCAATCC‑CCATCATTCCTTTTGAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCTCCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  1:1199436/238‑1 (MQ=255)

                                                                                                                                                                                                cgcTGGACGCCCTGGAACAGGAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  2:688347/238‑1 (MQ=255)

                                                                                                                                                                                                cgcTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  2:345024/238‑1 (MQ=255)

                                                                                                                                                                                                    ggACGCCCTGGAACGGCAGGCGGTTGGGTTCGCCGTCAACCC‑AAGGTATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGgtg                                       <  1:385854/239‑1 (MQ=255)

                                                                                                                                                                                                    ggACGCCCTGGAACAGCAGGCGGTTAGGTTTGCCGTCAATCC‑ACAGCATCCCTTTTTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAAATTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATTTGCGATAAAGTGG‑TAACGCGGTTTGGGTCTGACGACGTTTTATTTCAGATTAAAACAGTTgggg                                       >  2:519948/1‑237 (MQ=255)

                                                                                                                                                                                                     gacgcccctgtaagacagGCGGGTATGTGCGCCGTAAATCC‑CCAGCATCCTTTTATAACGTGGCAGGTTGACGGCCTAATCCAGCAGCAGGTTTTCCAGCAGGGGGGAAACGTTGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATAGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTACCAGCATAAAACCGTTGgtg                                       <  1:700298/223‑1 (MQ=255)

                                                                                                                                                                                                          ccTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGGAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCCTCACGCGGGAAACTTCCCTG‑ATATCTACCGGGGAATCCAGTTCCACCACAAACAACGAAATATCGTTTTTTTTTTCTGCGATAAAGTGG‑AAAAGCCGTTTGGGGGCTACCCCGTTTTCTTCCACAAAAAAAACGATGGGGTGGaaa                                 >  1:495310/1‑238 (MQ=255)

                                                                                                                                                                                                            tggagccgtagtgggttgttttCGCCGT‑AATCCGACGGCAGTCCTTTGGCACGTACGAGTTTCGCCGTCGTCTCCAGCAGCAAGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcag                               <  1:123330/219‑1 (MQ=255)

                                                                                                                                                                                                            tGGAACCGCAGGCGGTTAGTTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGTCTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcag                               <  2:741409/239‑1 (MQ=255)

                                                                                                                                                                                                            tGGAACAGCAGGGGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCCCGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcag                               <  2:33849/239‑1 (MQ=255)

                                                                                                                                                                                                            tGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcag                               <  2:151705/239‑1 (MQ=255)

                                                                                                                                                                                                            tGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcag                               <  2:486782/239‑1 (MQ=255)

                                                                                                                                                                                                            tGGAAAAGGAGGCGGTTAGGGTCCCCCGCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTACCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcag                               <  2:1265539/239‑1 (MQ=255)

                                                                                                                                                                                                             tgaagtaagggCGTTTAGGTCCCCCGTCAATCC‑CGAGCCTCCCTTGTTAACCTTGCATTTTATCCGACCACTCCAGCAGTAGGTTTTCCATCACGCGGGAAACTGAACTG‑ATGTCTATCTGGTAATCCAGTTCCGCCACAATCGACGAAAGATCGTTTTATTTATCTATTATAAAGTGG‑AAACTCGGTTTGTTGCGGACGACGTTTTCTTCCAGCATAAAACCGGTGGTGTTGAAcagc                              <  1:519948/230‑1 (MQ=255)

                                                                                                                                                                                                             ggaaatgcggggtggtatgTTCGCCCTCAACCC‑AAAGCATCCCTTTCTACCGGAGGAGTTTCTCCGCCGGCCCCCGCAGCAGGTTTTCCACCACGCGGGAAACTTAGATG‑ATATCTACCGGGTAAGCCAGGTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCGTCCAGCATAAAACCGTTGGTGTTGAAcagc                              <  1:37931/221‑1 (MQ=255)

                                                                                                                                                                                                             ggaaacactggCGGTTGGGTTCCCCGCCAAACC‑ACCACTCCCCTTTTGAACGGTGCCGGTTATCCGCCGTCTCCAGCAGCAGGTTTTCCATCCCGCGGGAAACTTCGCTG‑AGATCTACCGGGGAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcagc                              <  1:261077/230‑1 (MQ=255)

                                                                                                                                                                                                             ggAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcagc                              <  2:495310/239‑1 (MQ=255)

                                                                                                                                                                                                                acggcagGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcagcag                            <  2:204415/235‑1 (MQ=255)

                                                                                                                                                                                                                 aagcggGCGTTTTGTTTCCCCGTCAACCC‑CCAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGc                          <  1:105160/238‑1 (MQ=255)

                                                                                                                                                                                                                  gggggggggtttagTTCGCCGTCAATCC‑CCAGCATCCTTTTGTAACGTAGGCGTTTACCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTGCGCTG‑ATATCTACCGGGTAATCCAGGTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGcc                         <  1:1170209/226‑1 (MQ=255)

                                                                                                                                                                                                                    cagGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGGTAAAGTGG‑AAACGCGGGTTGGGGCTGACGACGTTTTTTTCCAGCCTAAAACCGTTGGGGTTGAACAACAGGCCCa                       >  2:1153800/1‑239 (MQ=255)

                                                                                                                                                                                                                    cagGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCGTAAAACCGTTGGGGTTGAACAGCAGGCCCa                       >  2:449405/1‑239 (MQ=255)

                                                                                                                                                                                                                    cagGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACCCGGGGAACTTCGCTG‑ATATCTACCCGGGAATCCCGTTTCAACACAATCGACGAAATATCGTTTTGTTTATCTGCGCTAAAGTGG‑AAAAGCGGGTTGTGGCTGCGGACGTGTTGTTTCACGATAAAAACGCTGGGGTTGGACAGCgggggcg                       >  1:682514/1‑235 (MQ=255)

                                                                                                                                                                                                                        cGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACCATCGACGAAATATCGTTTTGTTTATCTGCGGTAAAGTGG‑AAACGCGGGTTGGTGCTTACGCCGGTTTTTTCCAGCTTAAAAACGTTGGGGTTGCAAAGCAGGGCCAGATc                   >  2:132363/1‑239 (MQ=255)

                                                                                                                                                                                                                        cGGTTAGGTCCGCCGTCAACCC‑ACAGCATCCCGTTGTAACGTAGCAGTTTATCCGCCGTCTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGAt                    <  2:695500/238‑1 (MQ=255)

                                                                                                                                                                                                                            gagttttcccGTCAATCC‑CCAGCGTCCCTTTGTAACGATGCTGTTGTTCCGCCGATTCCAGCAGCAGGTTTTCCATCACGCGGAAAACTTCGGTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGGTTATCTGCGATAAAGTTG‑AAACGCGGTTTGGTGCTGACGACGGTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGAt                <  1:144877/230‑1 (MQ=255)

                                                                                                                                                                                                                                 tCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAATTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  2:1303501/205‑1 (MQ=255)

                                                                                                                                                                                                                                 tCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAATTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            >  1:1303501/1‑205 (MQ=255)

                                                                                                                                                                                                                                 accccctcaactc‑accGCATCCCTTTGGTAGCTAGCCGTTTTTCCGCCGACTCCAGCAGCAGGTTTTCCAGCACTCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCCCAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGcc          <  1:87987/227‑1 (MQ=255)

                                                                                                                                                                                                                                         aaTCC‑CCAGCATCCCTTTGTAACGTAGCAGTTTAACCGGCGACTCCCGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTT‑ATATCTACCGGGTAAGCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGa  <  2:1181072/239‑1 (MQ=255)

                                                                                                                                                                                                                                         aaTAC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGGCTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGGAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGa  <  2:892227/239‑1 (MQ=255)

TGGATATGGACTATTTTGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCC‑ACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTG‑ATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGG‑AAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGA  >  NC_000913/4587842‑4588313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`