Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 727,561 | A→C | 16.8% | V391G (GTG→GGG) | kdpA ← | potassium translocating ATPase, subunit A |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 727,561 | 0 | A | C | 16.8% | 140.4 / 2.3 | 60 | V391G (GTG→GGG) | kdpA | potassium translocating ATPase, subunit A |
Reads supporting (aligned to +/- strand): ref base A (37/10); new base C (2/11); total (39/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.28e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGTTGTTGTTAGCGGCGGATGACACGGCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTG > NC_000913/727330‑727785 | ccGTTGTTGTAAGGGGCGGATGCACCGGCGAACAGCCCTTCGCTAAAACCAGGCGGCCCAGGGTTGAGCATGGCCCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCcag < 2:159687/239‑1 (MQ=255) tgttgttAGCGGCGGATGACGCGGCTTAAAGCCTTTCCCAAAAACCAGGGGGGCCAGGGTTGGGCAGGGCCCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCCCCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTCCCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAgg < 2:153530/239‑1 (MQ=255) ttggcggggGGTAACAGGGCGCACGGCCTTTCGGAAACACCTTGGGGGCCGGGGTGGAGCCTGGCCTTCCGTCCGGCGTCGGTCATCATCGCCAACGCCGCTCCCATCAGCCCCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTCCGCCGATTTTTTTCCCCAGA‑TATCCCGGTGTAC‑GACCAATCTTC‑AGCCCGGCAATAAACC‑CCGCCAGCAGGACaaa < 1:742407/236‑1 (MQ=255) ttAGGGGCGGATGACACGGCGTACAGCACTTCGCTAAAACCATGGGGGCCAGGGTTGAGCATGGCGCTCCGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACaaa < 2:496165/239‑1 (MQ=255) cggcggATGACACGGCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCCGAATTGCCAGTGCAGCCAGTTTCATCTCCCGTACGTCCATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GCCCAATCATC‑AGCCCGGCAATAAACA‑CCGCCCGCAGGGCAAAAAGc > 2:1194036/1‑239 (MQ=255) ggCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGGGTAC‑GACCAATCATC‑AGCCCGGCACTAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTaaa > 2:619597/1‑239 (MQ=255) cGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTaaaa > 2:913544/1‑237 (MQ=255) aacaccctccGCAAAAACCAGGCGGGCCAGGGTTGAGCAGGGCGCTACGCCCGGCGTCGGTCATCATCGACAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTCCCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑cc < 2:1030331/229‑1 (MQ=255) cTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATTCCGTAAAGA‑CCAGAACCg > 2:1146772/1‑239 (MQ=255) ttCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCCGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCACTAAACA‑CCGCCAGCAGGCCAAACAGCATCATGCCGTAAAAA‑CCAAAACCg > 2:1186548/1‑238 (MQ=255) ttCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCAACATGCCGTAAAGA‑CCCAAACCGa > 1:685273/1‑239 (MQ=255) tAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGCCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCCGA‑TATTCCGGTGTCC‑GACCAATCATC‑AGCCCGGCAATAAACC‑CCGCCCGCAGGCCAAACAGCACAATGCCGGAAAAA‑CCAAAACCGAAAcc > 1:281247/1‑238 (MQ=255) aaaCCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGCCAAACCGCATCCTGCCGTAAAGA‑CCAGAACCGACAccccc > 2:894117/1‑239 (MQ=255) tGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGCGCCGTCAGTTTCATCTCGCGTACGTCGTTTTTTTTACCCAGA‑TATTCCTGTGTAC‑GACCACTCATC‑AGCCCGGCAATAAACA‑CCGCCAGCCGGAAAACCAGCATCATGCCGCAAAAA‑CCAAAACCCCCCCCCCCGaaac > 1:882007/1‑236 (MQ=255) aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTCCGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GCCCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGca > 2:100418/1‑187 (MQ=255) aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTCAAGA‑CAAAACACGACAACACCGAAaaccacaccaacaagtt > 1:459610/1‑227 (MQ=255) aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAACAGGAAAAACACACTCATTCCGTAAAAA‑CCAAAAACCACACCCCCCaaaaacaacacacccattgg > 1:543567/1‑221 (MQ=255) aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCAAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACTAACAGCATCATGCCGTACAGA‑CCACAACCGACCCCGCCGAACACAACTTCACCCAttt > 2:474597/1‑238 (MQ=255) aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCAAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGCAAAAAAGCCTCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAAttt > 2:772727/1‑238 (MQ=255) aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGGAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACC‑CCGCCAGCAGGACAAACAGCATCATGCCGCAAAAA‑CCAAAACCCACAACCCCGAAAAACAATACACCAAATTg > 1:595330/1‑239 (MQ=255) cATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGCCACCGCCGAACACCACTTCCCCAATTTGc > 2:960991/1‑238 (MQ=255) ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCCGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGC‑TATTCCGGTGTAC‑GACCCATCATC‑AGCCCGGCACTAAACA‑CCGCCAGCAGGACAAACAGCATCCTCCCCTAAAGA‑CCCAAAACGCCACCCCCGCACACCCCTCCACCaactaacaaac > 1:1116158/1‑230 (MQ=255) ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGACCACCACTTCACCAAATTGCATc > 2:295152/1‑238 (MQ=255) ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGAATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCa > 2:263832/1‑239 (MQ=255) cGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGACCCGACACAGCCGAACACCCCTTCACCAATTTGCATCAGCCCCATCGGc > 2:70655/1‑239 (MQ=255) cggctCGGGGTAACCAGATTTGCCAGTGCAGTCATTTTCATCGCGCGTTCGGCGTTTTTTTTACCCAGA‑TATTCCGGTGAAC‑GACCAATCACC‑AGCCCGGAAAAAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAAt < 2:281247/235‑1 (MQ=255) gCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCCATTTGCATAAGCCACATCGGCACCATGCCCCCGAGAGCGGTAAACGAATATTGCATCGCaacc > 2:171550/1‑237 (MQ=255) tCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAACGA‑CCAGAACCGACACCCCCGAACACCACTTCACCAATTTGCATCAGCCAAATCGGAACCCTGCCACCAACAGCGGaaaaaaaaacaaacacagcaaaaacc > 1:505228/1‑217 (MQ=255) ggTAACAAAATTTGCCAGTGCAGTCAGTTCCATCCCCGGTACGTCGTTTTTTTTCCCCAGA‑TTTTCCGGTGTAC‑GACCAATCATC‑AGCCCGGAAATAAACC‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACcgcg < 2:685273/238‑1 (MQ=255) gCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCcgtcg > 2:850500/1‑238 (MQ=255) cagtcagtTTCACCCCGCGTACGTCGATTTTTTACACCAGA‑TTTCCCGGGGAAC‑GACCAATCACC‑AGCCCGGCAAAAACCC‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCcgtcgt < 2:126244/238‑1 (MQ=255) ccttctgttctcctgctcttcGTCGATTTTTTCACCCAGA‑ATTCCCGGTCAAC‑CGAC‑ATCCTCGCGCCCGGCAATAAACC‑CCGCCAGCAGGACAAACAGCATCATCTCGTAAAGC‑CCCGAACCGACCCCGCCGAACCCCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCa < 1:263832/219‑1 (MQ=255) ttCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCCCAGGAAGCCGCCGTCGCCACGACCGc > 2:275533/1‑239 (MQ=255) catccggcttacggtgATTTTTTTCCCCAGA‑TTTCCCGGT‑TACAGCCAAACCACC‑CCCCCGGAAAAAAAAC‑CCGCCAGCAGGAAAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCaa < 2:543567/224‑1 (MQ=255) cATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGAGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACAGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGCAAGCGGTAAACGAATCATGCATCGCAATCACCCCGCCACAGGAAGccgccgccttaacgcccccca > 2:415455/1‑224 (MQ=255) ccgccgaccgtgttttttttCCCCAGATTGTTCCGGTGTAG‑CCCAAACCACC‑ACCCCGAAAAAAAACC‑CCGCCAGCGGGACAAAAAGCACCAGGTCGTAAAGC‑CCAGAACCCAACCCCCCGAACACCACTTCACCAATTTGCATCAGCCAAATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACa < 1:1194036/226‑1 (MQ=255) cggctACGTCGATTTTTTTCCCAGGA‑TATTCCGGTGAAC‑GCCCAACCATC‑ACCCCGGAAACAAACC‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAgg < 2:811559/235‑1 (MQ=255) cgTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGCAACCGACGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCCTGCATCCCAATCACCCCGCCCCCAGACACCGCCGCCGTCACGACCCCAAAAAgcgc > 1:1107956/1‑236 (MQ=255) ttccGTAGATTTTTTTCCCCAGA‑ATTTCCGGTGTAC‑GCCCAACCATC‑AGCCCGGCAATAAACC‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTa < 2:459610/236‑1 (MQ=255) tcattttttttCCCCAAA‑TATTCCGGTGTCC‑AACAAATCAAC‑ACCCCGAAAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGAc < 2:595330/235‑1 (MQ=255) gA‑TACTCCGGTGTAC‑GACCAAACCAC‑ACCCCGGCAAAAAACA‑CCGCCAGCAGGAAAAACAGCATCATGCCGTAAAGC‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACgg < 2:1290406/239‑1 (MQ=255) tattttCCGGTGTCC‑GACCAACTGAC‑CGCCGGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGACCCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACgg < 2:147378/235‑1 (MQ=255) atattgcgggggtAC‑GCCCAATCACC‑AGCCCGGAAAAAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACgg < 2:1003910/228‑1 (MQ=255) a‑tatTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCCAGCCCGCCAACAGGCTACTGCCCAGCACGCCGAAACgg > 2:327322/1‑238 (MQ=255) aagagccaCCAATCAACAAGCCAGGAAAAAACCC‑CCCCCCGCCGGACAAACAGCAGCGTGCCTGAAAGA‑CCAGACCCGCACCCCCCGAACACCCCATCACTAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCttt < 1:220853/232‑1 (MQ=255) gtAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAAt > 2:1011335/1‑161 (MQ=255) gtAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGCCTACGAACCGGCACGCCGAACCggaccccttac > 2:859029/1‑228 (MQ=255) tAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAAAAGGCGCCTGACCCGAACGCCGCAACGGCTCCCTTTAcc > 2:116689/1‑238 (MQ=255) c‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCcgtc > 2:1273632/1‑183 (MQ=255) cgcccaaacacgcgCCCGGC‑ATAAACACCCGCCAGCAGGACAAACAGCATCATTCCGTAAAGA‑CCAGAACCGACCCCGCCGAACCCCACTTCACCAATTTGCATCAGCCACATCGGCCCCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTc < 2:435039/226‑1 (MQ=255) ccAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGaa > 2:1061966/1‑80 (MQ=255) ccAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCCAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAACCGCCGTCGTCCCGACCGCAACCAAGCCACTGACCAGCACACCCAAACCGCCCCCTTTACCTCCCaa > 1:544065/1‑238 (MQ=255) acccaccggCCCGCAAATAACCC‑CCGCCAGCAGGACAAACACCATCATCCCTTAAAGC‑CCAGAACCGACCCCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGTCGCCGTCGTCACGACCTCAACCAGGCTACTGACCAGCACGCCTAAACGGCTCTCTTTACCTTCCatat < 2:412831/231‑1 (MQ=255) c‑aGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATAGCAATCACCGCGCCCACGGAAGCCGCCTCCGTACCGACCGCAAAAAGCCAACGCACCGCCACCCCAAAACGACCCCCTTAACCTTCAATAttaaat > 1:69068/1‑234 (MQ=255) gccagtcaaccaaCC‑CCCCCAGCAGGAAAAACATCACCATGCCG‑AAAGACCCAGAACCGACACCCCCCAACCCCACTGCACAAAATTGCCGTCGCCACCGCGGCACCAGGCCACCGAGGGCGGTAAACCAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATAGTGAtgctg < 1:4641/228‑1 (MQ=255) cccGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGAtgct > 2:923811/1‑237 (MQ=255) cccGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCCCTCTTTACCTTCCata > 2:220853/1‑229 (MQ=255) ggCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCACACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGTtgct > 2:4641/1‑234 (MQ=255) ggCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGGtgct > 2:982431/1‑234 (MQ=255) ggCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCCCTCTTTACCTTCCAAATTGAtgct > 2:820411/1‑234 (MQ=255) | CCGTTGTTGTTAGCGGCGGATGACACGGCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGA‑TATTCCGGTGTAC‑GACCAATCATC‑AGCCCGGCAATAAACA‑CCGCCAGCAGGACAAACAGCATCATGCCGTAAAGA‑CCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTG > NC_000913/727330‑727785 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |