Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 727,561 A→C 16.8% V391G (GTG→GGG)  kdpA ← potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913727,5610AC16.8% 140.4 / 2.3 60V391G (GTG→GGG) kdpApotassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base A (37/10);  new base C (2/11);  total (39/21)
Fisher's exact test for biased strand distribution p-value = 5.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTGTTGTTAGCGGCGGATGACACGGCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTG  >  NC_000913/727330‑727785
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
ccGTTGTTGTAAGGGGCGGATGCACCGGCGAACAGCCCTTCGCTAAAACCAGGCGGCCCAGGGTTGAGCATGGCCCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCcag                                                                                                                                                                                                                            <  2:159687/239‑1 (MQ=255)
    tgttgttAGCGGCGGATGACGCGGCTTAAAGCCTTTCCCAAAAACCAGGGGGGCCAGGGTTGGGCAGGGCCCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCCCCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTCCCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAgg                                                                                                                                                                                                                        <  2:153530/239‑1 (MQ=255)
         ttggcggggGGTAACAGGGCGCACGGCCTTTCGGAAACACCTTGGGGGCCGGGGTGGAGCCTGGCCTTCCGTCCGGCGTCGGTCATCATCGCCAACGCCGCTCCCATCAGCCCCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTCCGCCGATTTTTTTCCCCAGATATCCCGGTGTACGACCAATCTTCAGCCCGGCAATAAACCCCGCCAGCAGGACaaa                                                                                                                                                                                                                   <  1:742407/236‑1 (MQ=255)
         ttAGGGGCGGATGACACGGCGTACAGCACTTCGCTAAAACCATGGGGGCCAGGGTTGAGCATGGCGCTCCGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACaaa                                                                                                                                                                                                                   <  2:496165/239‑1 (MQ=255)
             cggcggATGACACGGCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCCGAATTGCCAGTGCAGCCAGTTTCATCTCCCGTACGTCCATTTTTTTACCCAGATATTCCGGTGTACGCCCAATCATCAGCCCGGCAATAAACACCGCCCGCAGGGCAAAAAGc                                                                                                                                                                                                               >  2:1194036/1‑239 (MQ=255)
                          ggCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGGGTACGACCAATCATCAGCCCGGCACTAAACACCGCCAGCAGGACAAACAGCATCATGCCGTaaa                                                                                                                                                                                                  >  2:619597/1‑239 (MQ=255)
                            cGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTaaaa                                                                                                                                                                                                 >  2:913544/1‑237 (MQ=255)
                               aacaccctccGCAAAAACCAGGCGGGCCAGGGTTGAGCAGGGCGCTACGCCCGGCGTCGGTCATCATCGACAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTCCCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGAcc                                                                                                                                                                                             <  2:1030331/229‑1 (MQ=255)
                                     cTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATTCCGTAAAGACCAGAACCg                                                                                                                                                                                      >  2:1146772/1‑239 (MQ=255)
                                      ttCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCCGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCACTAAACACCGCCAGCAGGCCAAACAGCATCATGCCGTAAAAACCAAAACCg                                                                                                                                                                                      >  2:1186548/1‑238 (MQ=255)
                                      ttCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCAACATGCCGTAAAGACCCAAACCGa                                                                                                                                                                                     >  1:685273/1‑239 (MQ=255)
                                           tAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGCCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCCGATATTCCGGTGTCCGACCAATCATCAGCCCGGCAATAAACCCCGCCCGCAGGCCAAACAGCACAATGCCGGAAAAACCAAAACCGAAAcc                                                                                                                                                                                 >  1:281247/1‑238 (MQ=255)
                                             aaaCCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGCCAAACCGCATCCTGCCGTAAAGACCAGAACCGACAccccc                                                                                                                                                                              >  2:894117/1‑239 (MQ=255)
                                                   tGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGCGCCGTCAGTTTCATCTCGCGTACGTCGTTTTTTTTACCCAGATATTCCTGTGTACGACCACTCATCAGCCCGGCAATAAACACCGCCAGCCGGAAAACCAGCATCATGCCGCAAAAACCAAAACCCCCCCCCCCGaaac                                                                                                                                                                         >  1:882007/1‑236 (MQ=255)
                                                                  aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTCCGTCGATTTTTTTACCCAGATATTCCGGTGTACGCCCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGca                                                                                                                                                                                                              >  2:100418/1‑187 (MQ=255)
                                                                  aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTCAAGACAAAACACGACAACACCGAAaaccacaccaacaagtt                                                                                                                                                          >  1:459610/1‑227 (MQ=255)
                                                                  aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAACAGGAAAAACACACTCATTCCGTAAAAACCAAAAACCACACCCCCCaaaaacaacacacccattgg                                                                                                                                                         >  1:543567/1‑221 (MQ=255)
                                                                  aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCAAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACTAACAGCATCATGCCGTACAGACCACAACCGACCCCGCCGAACACAACTTCACCCAttt                                                                                                                                                          >  2:474597/1‑238 (MQ=255)
                                                                  aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCAAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGCAAAAAAGCCTCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAAttt                                                                                                                                                          >  2:772727/1‑238 (MQ=255)
                                                                  aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGGAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACCCCGCCAGCAGGACAAACAGCATCATGCCGCAAAAACCAAAACCCACAACCCCGAAAAACAATACACCAAATTg                                                                                                                                                         >  1:595330/1‑239 (MQ=255)
                                                                    cATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGCCACCGCCGAACACCACTTCCCCAATTTGc                                                                                                                                                        >  2:960991/1‑238 (MQ=255)
                                                                       ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCCGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGCTATTCCGGTGTACGACCCATCATCAGCCCGGCACTAAACACCGCCAGCAGGACAAACAGCATCCTCCCCTAAAGACCCAAAACGCCACCCCCGCACACCCCTCCACCaactaacaaac                                                                                                                                                    >  1:1116158/1‑230 (MQ=255)
                                                                       ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGACCACCACTTCACCAAATTGCATc                                                                                                                                                     >  2:295152/1‑238 (MQ=255)
                                                                       ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGAATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCa                                                                                                                                                    >  2:263832/1‑239 (MQ=255)
                                                                                  cGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGACCCGACACAGCCGAACACCCCTTCACCAATTTGCATCAGCCCCATCGGc                                                                                                                                         >  2:70655/1‑239 (MQ=255)
                                                                                                                           cggctCGGGGTAACCAGATTTGCCAGTGCAGTCATTTTCATCGCGCGTTCGGCGTTTTTTTTACCCAGATATTCCGGTGAACGACCAATCACCAGCCCGGAAAAAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAAt                                                                                                <  2:281247/235‑1 (MQ=255)
                                                                                                                            gCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCCATTTGCATAAGCCACATCGGCACCATGCCCCCGAGAGCGGTAAACGAATATTGCATCGCaacc                                                                                               >  2:171550/1‑237 (MQ=255)
                                                                                                                               tCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAACGACCAGAACCGACACCCCCGAACACCACTTCACCAATTTGCATCAGCCAAATCGGAACCCTGCCACCAACAGCGGaaaaaaaaacaaacacagcaaaaacc                                                                                            >  1:505228/1‑217 (MQ=255)
                                                                                                                                   ggTAACAAAATTTGCCAGTGCAGTCAGTTCCATCCCCGGTACGTCGTTTTTTTTCCCCAGATTTTCCGGTGTACGACCAATCATCAGCCCGGAAATAAACCCCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACcgcg                                                                                         <  2:685273/238‑1 (MQ=255)
                                                                                                                                                      gCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCcgtcg                                                                      >  2:850500/1‑238 (MQ=255)
                                                                                                                                                       cagtcagtTTCACCCCGCGTACGTCGATTTTTTACACCAGATTTCCCGGGGAACGACCAATCACCAGCCCGGCAAAAACCCCCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCcgtcgt                                                                     <  2:126244/238‑1 (MQ=255)
                                                                                                                                                        ccttctgttctcctgctcttcGTCGATTTTTTCACCCAGAATTCCCGGTCAACCGACATCCTCGCGCCCGGCAATAAACCCCGCCAGCAGGACAAACAGCATCATCTCGTAAAGCCCCGAACCGACCCCGCCGAACCCCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCa                                                                   <  1:263832/219‑1 (MQ=255)
                                                                                                                                                               ttCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCCCAGGAAGCCGCCGTCGCCACGACCGc                                                            >  2:275533/1‑239 (MQ=255)
                                                                                                                                                                 catccggcttacggtgATTTTTTTCCCCAGATTTCCCGGTTACAGCCAAACCACCCCCCCGGAAAAAAAACCCGCCAGCAGGAAAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCaa                                                          <  2:543567/224‑1 (MQ=255)
                                                                                                                                                                 cATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGAGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACAGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGCAAGCGGTAAACGAATCATGCATCGCAATCACCCCGCCACAGGAAGccgccgccttaacgcccccca                                                          >  2:415455/1‑224 (MQ=255)
                                                                                                                                                                     ccgccgaccgtgttttttttCCCCAGATTGTTCCGGTGTAGCCCAAACCACCACCCCGAAAAAAAACCCCGCCAGCGGGACAAAAAGCACCAGGTCGTAAAGCCCAGAACCCAACCCCCCGAACACCACTTCACCAATTTGCATCAGCCAAATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACa                                                       <  1:1194036/226‑1 (MQ=255)
                                                                                                                                                                      cggctACGTCGATTTTTTTCCCAGGATATTCCGGTGAACGCCCAACCATCACCCCGGAAACAAACCCCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAgg                                                     <  2:811559/235‑1 (MQ=255)
                                                                                                                                                                        cgTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGCAACCGACGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCCTGCATCCCAATCACCCCGCCCCCAGACACCGCCGCCGTCACGACCCCAAAAAgcgc                                                   >  1:1107956/1‑236 (MQ=255)
                                                                                                                                                                         ttccGTAGATTTTTTTCCCCAGAATTTCCGGTGTACGCCCAACCATCAGCCCGGCAATAAACCCCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTa                                                  <  2:459610/236‑1 (MQ=255)
                                                                                                                                                                              tcattttttttCCCCAAATATTCCGGTGTCCAACAAATCAACACCCCGAAAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGAc                                             <  2:595330/235‑1 (MQ=255)
                                                                                                                                                                                              gATACTCCGGTGTACGACCAAACCACACCCCGGCAAAAAACACCGCCAGCAGGAAAAACAGCATCATGCCGTAAAGCCCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACgg                             <  2:1290406/239‑1 (MQ=255)
                                                                                                                                                                                               tattttCCGGTGTCCGACCAACTGACCGCCGGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGACCCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACgg                             <  2:147378/235‑1 (MQ=255)
                                                                                                                                                                                               atattgcgggggtACGCCCAATCACCAGCCCGGAAAAAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACgg                             <  2:1003910/228‑1 (MQ=255)
                                                                                                                                                                                               atatTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCCAGCCCGCCAACAGGCTACTGCCCAGCACGCCGAAACgg                             >  2:327322/1‑238 (MQ=255)
                                                                                                                                                                                                         aagagccaCCAATCAACAAGCCAGGAAAAAACCCCCCCCCGCCGGACAAACAGCAGCGTGCCTGAAAGACCAGACCCGCACCCCCCGAACACCCCATCACTAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCttt                     <  1:220853/232‑1 (MQ=255)
                                                                                                                                                                                                          gtACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAAt                                                                                                >  2:1011335/1‑161 (MQ=255)
                                                                                                                                                                                                          gtACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGCCTACGAACCGGCACGCCGAACCggaccccttac                    >  2:859029/1‑228 (MQ=255)
                                                                                                                                                                                                           tACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAAAAGGCGCCTGACCCGAACGCCGCAACGGCTCCCTTTAcc                  >  2:116689/1‑238 (MQ=255)
                                                                                                                                                                                                             cGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCcgtc                                                                       >  2:1273632/1‑183 (MQ=255)
                                                                                                                                                                                                               cgcccaaacacgcgCCCGGCATAAACACCCGCCAGCAGGACAAACAGCATCATTCCGTAAAGACCAGAACCGACCCCGCCGAACCCCACTTCACCAATTTGCATCAGCCACATCGGCCCCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTc               <  2:435039/226‑1 (MQ=255)
                                                                                                                                                                                                                 ccAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGaa                                                                                                                                                                           >  2:1061966/1‑80 (MQ=255)
                                                                                                                                                                                                                 ccAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCCAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAACCGCCGTCGTCCCGACCGCAACCAAGCCACTGACCAGCACACCCAAACCGCCCCCTTTACCTCCCaa            >  1:544065/1‑238 (MQ=255)
                                                                                                                                                                                                                    acccaccggCCCGCAAATAACCCCCGCCAGCAGGACAAACACCATCATCCCTTAAAGCCCAGAACCGACCCCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGTCGCCGTCGTCACGACCTCAACCAGGCTACTGACCAGCACGCCTAAACGGCTCTCTTTACCTTCCatat          <  2:412831/231‑1 (MQ=255)
                                                                                                                                                                                                                         caGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATAGCAATCACCGCGCCCACGGAAGCCGCCTCCGTACCGACCGCAAAAAGCCAACGCACCGCCACCCCAAAACGACCCCCTTAACCTTCAATAttaaat     >  1:69068/1‑234 (MQ=255)
                                                                                                                                                                                                                            gccagtcaaccaaCCCCCCCAGCAGGAAAAACATCACCATGCCGAAAGACCCAGAACCGACACCCCCCAACCCCACTGCACAAAATTGCCGTCGCCACCGCGGCACCAGGCCACCGAGGGCGGTAAACCAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATAGTGAtgctg  <  1:4641/228‑1 (MQ=255)
                                                                                                                                                                                                                             cccGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGAtgct   >  2:923811/1‑237 (MQ=255)
                                                                                                                                                                                                                             cccGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCCCTCTTTACCTTCCata           >  2:220853/1‑229 (MQ=255)
                                                                                                                                                                                                                                ggCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCACACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGTtgct   >  2:4641/1‑234 (MQ=255)
                                                                                                                                                                                                                                ggCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGGtgct   >  2:982431/1‑234 (MQ=255)
                                                                                                                                                                                                                                ggCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCCCTCTTTACCTTCCAAATTGAtgct   >  2:820411/1‑234 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  
CCGTTGTTGTTAGCGGCGGATGACACGGCGTACAGCACTTCGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACAGGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTG  >  NC_000913/727330‑727785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: