Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	82,217	C→G	9.4%	R438P (CGC→CCC)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,217	0	C	G	9.4%	244.0 / 3.2	93	R438P (CGC→CCC)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base C (35/45);  new base G (4/7);  total (39/54)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TTCGTTGTGTTGAGC‑TTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAA  >  NC_000913/81979‑82453                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               ttCGTTTGGTTGGGT‑TTTGCGTTTCACCTTTTTTTCGGCTTGTGGTGCACGCCAGAGATTGGTCAGAGCGGGCACCAGGGCTTGGGCAGATGACTCGACCATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGCTCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGc                                                                                                                                                                                                                                                <  1:908255/239‑1 (MQ=255) ttCGTTGTGTTGAGC‑TTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCCGTTCGACGTTATATTCAGGGATGc                                                                                                                                                                                                                                                >  2:116154/1‑239 (MQ=255) ttCGTTGTGTTGAGC‑TTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGc                                                                                                                                                                                                                                                <  2:916778/239‑1 (MQ=255) ttCGTTGTGTTGAGC‑TTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGc                                                                                                                                                                                                                                                >  2:512536/1‑239 (MQ=255) ttCGTTGTGTTGAGC‑TTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGc                                                                                                                                                                                                                                                <  2:542435/239‑1 (MQ=255)     gtggggtGAGCTTTTGCG‑TGCAACTATTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGtt                                                                                                                                                                                                                                            <  2:738245/233‑1 (MQ=255)       gtgtTGGGT‑TTTGTGTTGGCACTCTTTTTCTGCTGCCGCGGCACGACGGATTTATCTAGGACCGGGGAGCAGAGGTATGGCGGATCAATCGACAATAGCGGTAACCAGGCCGGAGACGTGCAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTaa                                                                                                                                                                                                                                          <  1:114921/239‑1 (MQ=255)        gtttttagtttttCGTTGCAGCTTTTTTTGCACTTCTGGGGCCCGCAAGAGAATGTTCAGAACGTCCACCAGGGCGTTGGCAGATGACTCGACAATATCGGTATCCAGGCCGACGCCGTGGAAGCGGCTACCGTTGTAGTTAGCGACGATATCCCCCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATGCAGTGATGCGGTTaa                                                                                                                                                                                                                                          <  1:5285/230‑1 (MQ=255)                gtttggcttGCAACTCTTTTTCGACTTCTGCGGCACCACAGATTGTGGTCAGAACGTGCACCAGGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGGTCGACGTTATATTCAGTGATGCGGTTAATTTCCTg                                                                                                                                                                                                                                   <  2:74102/231‑1 (MQ=255)                   tGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAg                                                                                                                                                                                                                               >  2:1147765/1‑239 (MQ=255)                   tGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCACCTTTACCGTGGCCTTTGGCGGTCAGGTTGGATTTCACCGCTTCGACGTTAAATTCATTGATGCTGTTAATTTCCttttaa                                                                                                                                                                                                                               >  1:1024429/1‑234 (MQ=255)                     cGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGGATTTCACCAGTTCGACGTTATATTTCGTGATGCGGTTAATTGCCTGTTAGAc                                                                                                                                                                                                                             >  2:1205002/1‑239 (MQ=255)                        tGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGc                                                                                                                                                                                                                          <  2:810664/239‑1 (MQ=255)                         gCAACTTTTTTTCGTCTTCTGGGGCACGCCAGGTTTTGTTCAGAACGGGCCCCATGGCTTTGGCAGGTGGCTCGGCAATATCGGTAGCCAGGCCGACGCCGTTGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCa                                                                                                                                                                                                                         <  1:192669/239‑1 (MQ=255)                           aaCTCTTTTTCGACCTCTGCGGCACGCCAGGTATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCAt                                                                                                                                                                                                                        <  2:735069/238‑1 (MQ=255)                              tttttttCGCCTTCTGCGCCACTCCATATTTTTCGTAGAACTGGCACCTGGGTTTTGGACGAAGACTCGACAATATCGGGAGCCAGGCCGACGCCGTTGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGGATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgac                                                                                                                                                                                                                    <  1:1302305/237‑1 (MQ=255)                              ttttttgtgattttcggcgcccGCCAGATATTGTCCAGAACGTGCCCGATGGCTTGGGCAGATGACTCGACAATAGCGGTGGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgac                                                                                                                                                                                                                    <  1:714091/218‑1 (MQ=255)                              tctTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgac                                                                                                                                                                                                                    <  2:1154579/239‑1 (MQ=255)                              tctTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgac                                                                                                                                                                                                                    <  2:1312903/239‑1 (MQ=255)                               ctTTTTCGACTTCTGCGGCACGCCAGAGATTGTTCAGACCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgacc                                                                                                                                                                                                                   <  2:886485/239‑1 (MQ=255)                                tttttccctttCTGCGGCACCCCAGATTTTTTTCAGACCGGGCCCCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgaccg                                                                                                                                                                                                                  <  1:644514/230‑1 (MQ=255)                                tttttCGACTGCTGCGGTCCGCCAGATATTGTTCAGAACGTGCCCCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgaccg                                                                                                                                                                                                                  <  2:30355/239‑1 (MQ=255)                                 ttttCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCCCCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCgaccgg                                                                                                                                                                                                                 <  2:1024429/239‑1 (MQ=255)                                              gcgCACGCCAAATTTTTTTCAAAACGGGGCCAATGGCTTTGGCAGATGACTCGAAAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTg                                                                                                                                                                                                    <  1:1241104/237‑1 (MQ=255)                                                          tatTGTTCAGACCGGGCACCAGGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCt                                                                                                                                                                                        <  2:364714/239‑1 (MQ=255)                                                          tatTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTggc                                                                                                                                                                                                  <  2:328117/229‑1 (MQ=255)                                                               ttCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCCTCTTTACCGTGGCCTTTGGCGGTCCGGCTGTATTTCACCATTTCGACGTTTTATTCAGTGTGTGGGTTAATTGCCTGTTAGACGGCCCCGACCGGACCGTTACCGTTGGGGGCCTCTTCTTTGaa                                                                                                                                                                                   >  1:271810/1‑238 (MQ=255)                                                                    aaCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTATCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTAAGGGTGTATTTCACCAGTTCTACGCTATATTCAGTGATTCGGTTAATTGCCTGAACGCCGGCAACGACCGGCCCGTTACCGTTGGCGGCTTCTGCTTTTActttt                                                                                                                                                                               >  1:459741/1‑236 (MQ=255)                                                                    aaCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCCGGCTGTATTTCACCAGTTCGACGTTATATTTAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGGTTCTGCTTTGActtct                                                                                                                                                                               >  2:417722/1‑238 (MQ=255)                                                                    aaCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCCCCCGTTCGACGTTATATTCAGTGATCGGGGTAATTGCCTGATATACGCGCTCGACCGGCCCGTTACCCTTGGGGGCTTCTGCttttgtctttt                                                                                                                                                                              >  1:328117/1‑231 (MQ=255)                                                                    aaCGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGActtct                                                                                                                                                                               >  2:1204995/1‑238 (MQ=255)                                                                        tGCCCCTTGGGTTTGGGAGATGACTGTGCGATACCGGTAGCCAGGCCGGCGCCGTGGGAGCGGCGACCGTTGGAGTTAGCGACGATATCCCACTGCCCCAGCCCATCTTTTCCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCg                                                                                                                                                                            <  1:609596/237‑1 (MQ=255)                                                                         gCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCCGTTCGACGTTATATTCAGTGTTGCGGTTAATTGCCTGATACACGGCATCACCCGGACCCTGACCATGGGCGGCTTTTCCTTTTACTCTTTCG‑Cca                                                                                                                                                                        >  1:8018/1‑239 (MQ=255)                                                                         gCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCATTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑cc                                                                                                                                                                         >  2:609596/1‑238 (MQ=255)                                                                         gCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGGCAGGCTGTATTTCACCCGTTCGACGGTATATTCTATGATGCGGTTAATTGCCTGATAAAAGGCATCGACCGGGCCCTTAACGTTGGCGGCTTGTGCTTTGActttgtggcca                                                                                                                                                                         >  1:597485/1‑231 (MQ=255)                                                                          cACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑Cca                                                                                                                                                                        <  2:309879/238‑1 (MQ=255)                                                                              aTGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGTTAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTCTTTCG‑CCCCAgg                                                                                                                                                                    >  2:333725/1‑238 (MQ=255)                                                                                  cTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGccg                                                                                                                                                                 >  2:970841/1‑236 (MQ=255)                                                                                     tGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTCCGACGTTATATTCAGGGATGCGGTTAATTTCCTGAAAGACGGCACCGACCGGACCGTTACCTTTGGTGGCCTCTTCTTTGATTTCTTCGCCCACA‑GCCAGTTTg                                                                                                                                                            >  1:1132591/1‑239 (MQ=255)                                                                                            tGACTCGGCAATTGCGGTAGCCAGGGCGCCGCCGTGGAAGCGGCGACCGTTGTCGGTAGCCACGATCTCCACCTGACCCAGCCCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGAcggcgg                                                                                                                                                     <  2:171588/239‑1 (MQ=255)                                                                                              tcTCGACCATTTCGGTTGCCCGGCCGAGGCCGTGGAAGCGGGGCACGTTGTAGTTGGGGACGATATCCCCCTGGCCCCGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGAcggcggcg                                                                                                                                                   <  1:1292365/238‑1 (MQ=255)                                                                                                      ttttgggTGGCCAGGCCGGCGCGGGTGAAGCGTGGCCCGTTGTCTGTAGGGACCATTCCCCTCTGCCCCAGGCCTTCTTTGCCTGGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGAGTCGGTTAATTGCCTGATAGACGGCATCGCCCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGat                                                                                                                                          <  1:696682/234‑1 (MQ=255)                                                                                                         tCGGTAGCGCGGCCGACGCTGTGGAAGGGGGGACCTTTGTAGTTCGCGACGATATCCCCGTGACCCAGCGCATCTTTCACGTGGCCGTGGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCTGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGata                                                                                                                                         <  1:1205002/237‑1 (MQ=255)                                                                                                           ggTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGt                                                                                                                                     <  2:1066419/239‑1 (MQ=255)                                                                                                             ttgcacggCCGGCGCCGTGTAAGCTGCGGCGGTTGTAGCTACGGCCGTTTTCCACTGGCCCCACCGCATCTGTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTGGTATTCAGTGATGGGGCGAATTGCCTGATAGGCGGCATCGGCCGGATCTGTACCTTTGGGGGCTTCTGCTTTGATATCTTCG‑CCACAGCCCAGTTTGGCGGGGGCG‑GTGGCGATATCGTTa                                                                                                                                   <  1:732835/233‑1 (MQ=255)                                                                                                              aGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGGGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCCCAGGCCAGTTTGACGGCGGCG‑GGGGccaatacggtaa                                                                                                                                  >  2:1097714/1‑228 (MQ=255)                                                                                                                ccggccccgcggcggggAAGGGGGGGCCCTTGTAGTTAGCGACGTTATCCCCCTGACCCAGCCCATCTTTACCTGGGGCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGAGGTTAATTGACTGATAGACGGCATCGCCCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTagag                                                                                                                                <  1:1204995/224‑1 (MQ=255)                                                                                                                         cGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGc                                                                                                                       >  2:461324/1‑239 (MQ=255)                                                                                                                         atgtgtaaaagagacagccGTTGTAGGTAGCGACGATAGCCCCCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTg                                                                                                                        <  2:545100/221‑1 (MQ=255)                                                                                                                            cGTGGAAGCGGCGACCGTTGGAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACg                                                                                                                    <  2:1319940/239‑1 (MQ=255)                                                                                                                              tGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGGGGTTAATTGCCTGATAGACGGCCTCGACCGGACCGTTACCGTTGGCGGCTTTCGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GGTGCGGTATCGTTTGAGcccaaacacacgcg                                                                                                                  >  1:1319940/1‑227 (MQ=255)                                                                                                                                gAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTg                                                                                                                 <  2:8018/238‑1 (MQ=255)                                                                                                                                      gcgACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACCGGCCAGTTTGCCGGCGGCC‑GGTGCGGTTACGGTAGAGCCAGACCCCACGCTGAAGaaa                                                                                                           >  1:950208/1‑238 (MQ=255)                                                                                                                                         ccggTTGTATTGAGCGCCGATTACCGCGTTACCCGTCGCAGCTCTACCGTGGTCTTTGGCGGTCAGGTTGTATTTCACCAGTTCGCCGTTTTATTCAGTGTTGCCGTGAATTGCCTGATAGACGGCATCTGCCGGACCGTTACCGTTGACGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTGGACGGCGGCG‑GGGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCa                                                                                                       <  1:691406/236‑1 (MQ=255)                                                                                                                                         aCCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCCCAGGCCAGTTTGACGGCGGCG‑GTGGCGCTATCGTTAGAGCCAGACTGCACGCTGGAGTAATCCa                                                                                                       >  2:739435/1‑239 (MQ=255)                                                                                                                                                       cgacgaTATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGGTATCTTTTTGGCCAGACTGCACGCTGAAGGAATCCCAACGGAAATGCTcc                                                                                         >  2:1159677/1‑239 (MQ=255)                                                                                                                                                           catatcccctgtccccGGCGCATTTTTTCCGTGGCCTTGGGCCGTCTCGCTGTATTTCAGCAGGTCGTCGTTATATTCAGTGAGGCGGTTCATTGCCTGATAGACTGCATCGGCCGGACCGTTACCGTCGGCGGGTTTTGCTGTGACTTCTTCG‑CCTCAGGCCAGTTCGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGct                                                                                     <  1:155616/226‑1 (MQ=255)                                                                                                                                                                ccACCCTACCCAGCGCCTCTTTACCGTGGCCTTTGGCGGTCAGGCGGTATTTCGCCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCtcttct                                                                                <  2:53389/239‑1 (MQ=255)                                                                                                                                                                  tcttgtccccgggcATTTTTATCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCATTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTtg                                                                              <  1:414739/227‑1 (MQ=255)                                                                                                                                                                       aCCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTgctgct                                                                         >  2:245305/1‑239 (MQ=255)                                                                                                                                                                       aCCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCCCTTCTTgctcct                                                                         >  2:128019/1‑239 (MQ=255)                                                                                                                                                                       aCCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GGGGCGCTAACGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGCCGGAAAATGTCCCGCGCTTCTTgctgct                                                                         >  1:812406/1‑239 (MQ=255)                                                                                                                                                                            gcgcATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATCGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GGTGCGATATCGTTAGAGCCCGACTGCCCCGTGAAGAAATCCACGCGGAAATGCTCCGGCGCTTCTTTCCGCTTACCg                                                                    >  1:173828/1‑239 (MQ=255)                                                                                                                                                                                  cTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCCGACGGAAATGCTCCGGGCCTTCTTGCTGCTTACCGGTGAAg                                                              >  2:5074/1‑239 (MQ=255)                                                                                                                                                                                       ccGTGGCCTTTGGGGTTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAg                                                         <  2:271810/239‑1 (MQ=255)                                                                                                                                                                                       ccGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTGCCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAg                                                         <  2:459741/239‑1 (MQ=255)                                                                                                                                                                                       ccGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAg                                                         <  2:683270/239‑1 (MQ=255)                                                                                                                                                                                       ccGGGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAg                                                         <  2:173828/239‑1 (MQ=255)                                                                                                                                                                                          tGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGGAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAgcg                                                       >  2:434962/1‑238 (MQ=255)                                                                                                                                                                                            gCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGCCGCCATCGACCGGACCGTTACCGTTGCCGGCTTCTGCTTTTACTTCTTCG‑CCACAGGCCCGTTTTCCGGGGGCGTGGGGGGATTCCTTTAG‑GCGCAACTTCACCCCGCAGCAACCCAGAAGGCAAAGCCCCGGCTCTCTTTGCTCCTTcccccttaagcacccaccc                                                     >  1:488222/1‑222 (MQ=255)                                                                                                                                                                                            gCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCCCGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGGGcc                                                     >  2:861147/1‑238 (MQ=255)                                                                                                                                                                                            gCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCCGACGGAAATGCTCCGGCCCTTCTTGCTGCTTACCGATGAAGGCCAGcccc                                                     >  2:549824/1‑238 (MQ=255)                                                                                                                                                                                            gCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCCACCGGAAATGCTCCGGCGCTTCTTTCTGCTTACCGCTGACGGCCCGCGcc                                                     >  2:485081/1‑238 (MQ=255)                                                                                                                                                                                            gCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGcc                                                     >  2:1004925/1‑238 (MQ=255)                                                                                                                                                                                              cTTTGGCGGTCAGGCTGTATTTCCCCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTc                                                   <  2:1225970/238‑1 (MQ=255)                                                                                                                                                                                                  ggCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGAt                                              <  2:950208/239‑1 (MQ=255)                                                                                                                                                                                                  ggCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGGGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGAt                                              <  2:93864/239‑1 (MQ=255)                                                                                                                                                                                                     gttccggCTGTTTTTCCCCCGTTCGTCGTTTTTTTCAGTGATGCGGTTAATTGCCTGATAGCCGGCACCGACCGGACCGTTTCCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGt                                           <  1:512536/234‑1 (MQ=255)                                                                                                                                                                                                     cgTCAGGGTGTATTTCACCAGTTCGACGTTATGTGCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCg                                            <  2:522985/237‑1 (MQ=255)                                                                                                                                                                                                      gTCAGGCTGGATTTCACCAGTTCGACGTTATATTCAGGGATGCGGTTAATTTCCTGATAGACGGCATCGACCGGCCCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGt                                           <  2:612753/238‑1 (MQ=255)                                                                                                                                                                                                       tctggtttttttttACCGGTTCGTCGTTATATTCAGGTATGGGGTTTATTGCCTGATAGGCGGCATCGCCCGGACCGTTACCGTTGGGGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGGTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTa                                          <  1:226745/228‑1 (MQ=255)                                                                                                                                                                                                                   tcccccttttcctgTTTTATTTGGTGATTGGGTTAATTGCCTGATTGACGGCATCGCCCGGACCGTTACCTTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGa                             <  1:1097714/226‑1 (MQ=255)                                                                                                                                                                                                                     aCCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGGACCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCCCTTCTTTGTGCTTACCGATTAAGGCCCGGGCCTCCCGATCGTAATCCAACACCtaa                             >  1:1309541/1‑235 (MQ=255)                                                                                                                                                                                                                     aCCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGa                             >  2:68528/1‑237 (MQ=255)                                                                                                                                                                                                                         gTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGCCTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGTTGAAGGCCAGCGCCTCCAGATCGTAATCAAAAACCTGACCtttt                       >  2:271389/1‑239 (MQ=255)                                                                                                                                                                                                                           tCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCTGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCttttt                      <  2:378219/238‑1 (MQ=255)                                                                                                                                                                                                                             gACGTTATATTCAGTGATTGGTTTAATTGCCTGATAGACGGGATCGACCGGACCGTTACCGTCGGCGGTTTCTGCTTTGACTTCCTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCCCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGt                   <  2:179005/239‑1 (MQ=255)                                                                                                                                                                                                                              aCGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATTTCCTTTAAGCCAGACTTCACGCTGAAGGAATCCGGACGGAAAAGCCCCGGCTCTTCTTGCTGCTTACCGATTAAAGCCAGCACCTCCAGATACGAATTAAACACCTTAACTTTTTTGt                   >  1:655392/1‑238 (MQ=255)                                                                                                                                                                                                                                  tttttttatgtaTGGGGTTAATTGCCGTATGGGCGGCTTCGCCGCGACCGTTCCCGTTGGCGGCTTCTGCGTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGcc              <  1:116154/228‑1 (MQ=255)                                                                                                                                                                                                                                  ttttttCAGTGGTGGGGTTAATTGCCTGATAGACGGCATCGCCCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGcc              <  1:245305/235‑1 (MQ=255)                                                                                                                                                                                                                                    tttttggTGATGTGGTTATTTCCTTTATGGACGGCAACGCACGGTCGTTTCCCGTTGGGGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTGTGTCCGCCAg            <  1:1063211/233‑1 (MQ=255)                                                                                                                                                                                                                                    tatTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAAAACCTGACCTTTTTTGTCCGCCa             >  2:597337/1‑238 (MQ=255)                                                                                                                                                                                                                                       tgggtggtgCGGTTAAGTGCCTGAAAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCtt         <  2:488222/236‑1 (MQ=255)                                                                                                                                                                                                                                       tcggtggtgGGGTTAATTGCCGGAATGGCGGCAGCGACCGGGCCGTTCCCGTGGGCGGCTTCTGCTTTGACTTCGTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCtt         <  1:861147/236‑1 (MQ=255)                                                                                                                                                                                                                                       tCAGTGAGGCGGTTAATTGCCTGATAGGCGGCATCGGCCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCtt         <  2:655392/239‑1 (MQ=255)                                                                                                                                                                                                                                         aGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGGAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCa       >  2:1063211/1‑239 (MQ=255)                                                                                                                                                                                                                                           tGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GGTGCGGTATCGGTAGAGCCCGACTGCCCGCTGAAGTAATCCCGACGGAAATGATCCGGCCATTCTTGCGGCTTGCCGCGCAATGCCAGCCCCCCCACATAGTCATCAAACCCCTCACCTTTTTTTCCCCCCAACttaccg     >  1:943984/1‑235 (MQ=255)                                                                                                                                                                                                                                              tGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGGGCCTCCAGATCGTAATCAAAACCCTGACCTTTTTTGTCCGCCAGCTTCAGGaaa  >  2:695658/1‑239 (MQ=255)                                                                                                                                                                                                                                                cGGTTAAATGCCTGATAAGCGGCATCGACCGGCCCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGa    <  2:457793/235‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               TTCGTTGTGTTGAGC‑TTTGCGTTGCAACTCTTTTTCGACTTCTGCGGCACGCCAGATATTGTTCAGAACGTGCACCATGGCTTTGGCAGATGACTCGACAATATCGGTAGCCAGGCCGACGCCGTGGAAGCGGCGACCGTTGTAGTTAGCGACGATATCCACCTGACCCAGCGCATCTTTACCGTGGCCTTTGGCGGTCAGGCTGTATTTCACCAGTTCGACGTTATATTCAGTGATGCGGTTAATTGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCG‑CCACAGGCCAGTTTGACGGCGGCG‑GTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAA  >  NC_000913/81979‑82453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑