Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 811,721 T→G 24.7% V67G (GTG→GGG)  bioC → malonyl‑ACP O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913811,7210TG24.7% 78.2 / 3.0 42V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (7/22);  new base G (9/3);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 4.31e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCA  >  NC_000913/811484‑811949
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
tgggaggaaGGATATCGACCGTCTGGTGGGGGTGCTGCAGGGCAACGGTTAATAACCAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACCCTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTg                                                                                                                                                                                                                                        <  2:759186/231‑1 (MQ=255)
 tatacgCAGGATCTCCACCGTCGGGTGGGGGTGCGGCTGGGAACCGTTTAAAAAACAAGCCCTGGCGGGGGCATTTGGGCGGGGAGCCCCACACTATGAGCAACCTGCAGATTTACAGCCCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATCCCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                       <  1:1203913/234‑1 (MQ=255)
   aaggCAGGATATGCACCGTCGGCGGGGGGGTCGGCAAGGAAACGGTTAATAAACAAGCCATGGCAGCGGCATTTGGGCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCCCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAATTCCACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACg                                                                                                                                                                                                                                    <  2:78180/236‑1 (MQ=255)
   aaggCAGGATAGCGCCCGTCTGCTGGGGGTGCTGCATGGCAACGGTTAAAAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                       <  2:1025563/234‑1 (MQ=255)
   aaggCAGGATACGGACCGTCGGCGGGAGGTGCTGCATGGAAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc                                                                                                                                                                                                                                      <  2:430331/235‑1 (MQ=255)
           atagggACCGTTTGCTGGGGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                       <  2:766952/224‑1 (MQ=255)
                    gTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCGCGCGCCAGTGGCGGccccgtctcttataca                                                                                                                                                                                                                   >  2:821719/1‑225 (MQ=255)
                     tCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTTTGGACCTGGCTGGCTGAGCCGCCACCGGCGGGACCGCCACCCGCCCGGGGCCGGCCTTAGAttctccttt                                                                                                                                                                                                                   >  1:766952/1‑231 (MQ=255)
                      ctgctgGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCCTAGATCTCTccccg                                                                                                                                                                                                                  >  2:1018047/1‑238 (MQ=255)
                      ctgctgGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGACAGGCCCTTGATCTCTcgccc                                                                                                                                                                                                                  >  2:1074750/1‑237 (MQ=255)
                                 gctgcATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGGCACGCGCCGGGGACGGGCTTTGATCTCTCGCCGCCAAAGCTTGt                                                                                                                                                                                                       >  2:1201345/1‑238 (MQ=255)
                                 gctgcATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAACCGCCACTGGCGGGAAAGTCACGGGCGGGGCAAGGCCGTTAAACTCCCGCCGCCAAGGCtgtgt                                                                                                                                                                                                      >  1:1315449/1‑235 (MQ=255)
                                     cgtgacAACGTTTAAAAAACAAGCCATGGAGGGGGGTTTGGGTGGGGCAGCCGACACATATGGGAAAACAGGAGACTTACAGCGCCAGGGTGCTGACGCTTTCCTGGCAATGTTTCCAAGGCGTAAATACCCCCACGTACTGGACGGGGGTTGTGGACCGGGCTGGATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAgg                                                                                                                                                                                                  <  1:727209/234‑1 (MQ=255)
                                      aTGGCAACGGTTAATAACCAAGCCATTGCCGCGGCATTTGGTCGGGCAGCCGCACCCTATGACCAACATGCAGATCTACGGCGCCAGAGTGCTTACCCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGAGGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAgg                                                                                                                                                                                                  <  2:320625/238‑1 (MQ=255)
                                               gTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCACGCCCAGGGGACGGCCCTAGATCTCTCCCCGCCAATGCTTTTTCAGGCACGcccaaa                                                                                                                                                                                        >  2:147538/1‑235 (MQ=255)
                                                        cAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCCCGCCGGGGAGGGCCTTATGTTCCTCGCCCCCCACGCTTGttttgcgacgcgcgaaaggaacggc                                                                                                                                                                               >  1:1050590/1‑216 (MQ=255)
                                                         cagccttgtcggggggttttGGGCGGGCGCCCCCACCCTTAGAGAAACCTGGAGATCCACAGGGCCAGTGGGCTGACGCTTACCGGGCAATGCTTCCACAGCGTAAATACCCCCCGGTCCTGGACGGGGGTTGGGGACCTGGGTGGATGGGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa                                                                                                                                                                              <  1:1074750/222‑1 (MQ=255)
                                                         aaGCCATTGCAGGGGCTTTTGGTCGGGCAGCCCCACACTATGAGCAACATGCAGTTCTAAAGCGCCAGAGGCCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATCCCCCCACGTACTGGACGGGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa                                                                                                                                                                              <  2:18854/239‑1 (MQ=255)
                                                          aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGTTACGCGCCGGTGACGGGCGTAGATCTCTCGCCGCCCAAGCCTGTTCAGGCGCGCCCGAAGGGTGCCCCa                                                                                                                                                                              >  2:181874/1‑238 (MQ=255)
                                                          aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGGCCCGCGCACGGGGCGGGCTTAGATTTCTCCCCGCCCAGGCCTGTTCGGGGCCCCCAGAAGGAGGCCGCa                                                                                                                                                                              >  2:96491/1‑238 (MQ=255)
                                                                   aGCGGCATTTGGCCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTAt                                                                                                                                                                    <  2:149655/238‑1 (MQ=255)
                                                                     cGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAGCTCTCGCCGCCAATGCTTGTTTAGGCACGCCAGAAAGATGCCGCGGACCCTTATCTg                                                                                                                                                                 >  2:851464/1‑239 (MQ=255)
                                                                       ggttttGGTGGGGACCCCGCACACTGTGAGCAAACTGACATCCTCGAGCGCAGGGGTGCTCACGCCTGCGTGGCAATGCTCCACCAGCGAAAATCCCCCCGGGTGCGGGACGGGTGTGGGGGACCGGGCGGGATGAGCCGCCCCGGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTgg                                                                                                                                                                <  1:908493/235‑1 (MQ=255)
                                                                          tttGGTCGGGCAGCCCCACACTGTGAGCAACATGCAGATCTCCAGCGCCAGAGGTCTGCCGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTCCGGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCggg                                                                                                                                                            <  2:871712/239‑1 (MQ=255)
                                                                                   gCAGCCGCCCACTATGAGAAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACGGGCAATGCTTCCACAGCGTAAATCCACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCg                                                                                                                                                    <  2:451143/238‑1 (MQ=255)
                                                                                                          aTGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCAGGTGACGGCCCTAGa                                                                                                                                                                                                                            >  2:363828/1‑145 (MQ=255)
                                                                                                          aTGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGAAGCCGCAGACCATTATTCGCGGGGAGATATCGAATCCCTGCCGTTAGCGACTggg                                                                                                                             >  2:684784/1‑237 (MQ=255)
                                                                                                                           ccAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCGTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCCTTATCCTGCGGGGGATATTGAATACCCGCCGTTAGCGACTGCGACGGTCGATCttt                                                                                                                >  2:178411/1‑235 (MQ=255)
                                                                                                                            cggagTGCTGACGCCTTCCTGGCAATGCTTCCACCGCGTAAATACACCCACGTACGGAGCGCGGGTTGTGGACCTGGCTGATTGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                       <  2:1088639/116‑1 (MQ=255)
                                                                                                                                     gACCCCTTACGGGAATTGCTCCCCCGGCGTAAATACCCCCACGTCCGGGCCGCGGTTTGTGGCCCTGGCGGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCCCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAAtct                                                                                                   <  2:1050590/239‑1 (MQ=255)
                                                                                                                                                  cAATGCTCCCCAGGCTTAAATCCACCCACGTACGGGACGGGGTTGGGGGCCGGGGCTGGATGGGCCGCCCCTGGCGGGAACGTCCCGCGAAGGTGACGGCTTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGcagtgcagtg                                                                                       <  2:874201/238‑1 (MQ=255)
                                                                                                                                                                cGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGGGATATCGAATCCCTGCCGTTAGCGGCAGCGACGTTCGATCTTGCATGGGGCAATCTCCCAGTGCAGCGGGGGGGTGATTTAt                                                                        >  2:954520/1‑238 (MQ=255)
                                                                                                                                                                    aaTACACCCACGTACGGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa                                                                                                                                                                              <  2:253015/134‑1 (MQ=255)
                                                                                                                                                                           cccggTACGGGCCGCGGGTTGGGGACCGGGTGGGAGGACCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTcc                                                            <  2:1156628/235‑1 (MQ=255)
                                                                                                                                                                             aCGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTGACGCGCAGGGTACCGCCTTAGATCTCCCCCCGCCAAACCTTTGTCAGGCCCGCCCGAAGGGTTGCCCAGACCCATTACTGGCGGGGGGTATCCAAACCCCGCCGCTAGCGAGCGCCACACTCTGTCTTTCATGGAGCGATACCACAGAGGAGCGGGGCGGTAAATTTAtacccaacacaccg                                                           >  1:160577/1‑226 (MQ=255)
                                                                                                                                                                                 gcgggccggggtttGTGGCACTGGTGGGAGGCGCCCCCACTGGCGGGAACGTCACGGGCAGGTGACGGCCTTAGATCCCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAg                                                       <  2:461819/226‑1 (MQ=255)
                                                                                                                                                                                 acggggcggggggTGGGGCCCTGGCTGGTTGAGCCCCCACGGGGGGGACCGTCACGCGAAGGGGACGGCTTTAGATCTCCCGCCGCCAATGCTTGTTCAGGCCCGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAg                                                       <  2:767879/229‑1 (MQ=255)
                                                                                                                                                                                                tggcgcggggggggggggcccgccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGgtggtg                                        <  2:430605/220‑1 (MQ=255)
                                                                                                                                                                                                   gcccgggggggagggcccgccACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCGGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTgcg                                      <  2:935938/222‑1 (MQ=255)
                                                                                                                                                                                                                                gAACGTCCCGGGAGGGGGAGGGCTTTAGACCTCTCGCCGCCAATGGTTGTGCAGGCCCGCCAGAAGGATGCCGCAGACCATTAGCTGGGGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGc         <  2:595960/238‑1 (MQ=255)
                                                                                                                                                                                                                                    gTCACGCGCAGGTGCCGGCCTTAGATCTCTCGCCGCCAATCCTTGTTCAGGCCCGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCaa                                <  2:607673/211‑1 (MQ=255)
                                                                                                                                                                                                                                      cACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGGGGGCGCGTTTACCACGCTGGTGCa  >  2:692477/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
TGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGTGCA  >  NC_000913/811484‑811949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: