Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 825,248 G→C 20.2% G287G (GGC→GGG ybhR ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913825,2480GC20.2% 111.6 / 3.4 62G287G (GGC→GGGybhRputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base C (11/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 2.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAACC  >  NC_000913/825014‑825482
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
aCGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGGGAAACGTAACCGGAAAGGAGAATGGCGGGCACCTTCAAACCAAAAACGccca                                                                                                                                                                                                                                           >  2:442325/1‑237 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACGATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCCTAAAGAAAAACAACGCGATaaaag                                                                                                                                                                                                                                     >  2:1170016/1‑236 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGATCATAAAGAAAAAAACGCCGATAAACg                                                                                                                                                                                                                                     >  2:932817/1‑238 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAAAACGcccgaaaaag                                                                                                                                                                                                                                     >  2:616357/1‑230 (MQ=255)
       aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGGTAATCCAAGTTCGGTTTTGCAGCCCTACCGGCCGGGTTTCCACCGGAGAAACGTAACCGGAAAGGGGAAAGGCGGGCATCCTAAAGAAAAAAACGCCCAGaaaaa                                                                                                                                                                                                                                     >  1:919682/1‑236 (MQ=255)
        aCATCGGGTACCCCTCTGCCCCTGTCGTGGCCGTTACCACCAGTAGCGGCCACAACCTATCCCAAACAATACCCAGACTCGCATCCTTCAAATAAATCTGTTTGGTAATGTCCGTAAAGGGGCAAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAAAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGAAAAACACGCCGATAAACGcc                                                                                                                                                                                                                                   <  2:586059/239‑1 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCGTCATAAAGAAAAAAAACCCGATAAAAGCct                                                                                                                                                                                                                                  >  2:260705/1‑236 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCCTAAAGACCAAAACACCCGTAAACGCccgct                                                                                                                                                                                                                               >  2:170512/1‑239 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACCCCCATCAACGCct                                                                                                                                                                                                                                  >  2:148078/1‑236 (MQ=255)
            cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGAATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGATCATAAAGAGCAAAACCCCGATAAAAGccc                                                                                                                                                                                                                                  >  2:937052/1‑235 (MQ=255)
             gcgTACGCCGCTGACCCTTCCTTGGCCTTAACCCCAAGTAGCGGCCAAAAACTATCCCACAAAATATCCAGCCTCGCTTCCTTCAAATAAACCTGTTGGGTAATGTCCGTAAAGTGGCAAATAGGGTTAACCCACTTAAGGTTTGGCAGCCATACCGGCATGTTTTCCACCGGAAAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCctgctg                                                                                                                                                                                                                              <  2:620549/239‑1 (MQ=255)
              cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACCCCGGTAAACGCCTGCcgt                                                                                                                                                                                                                             >  2:1166454/1‑239 (MQ=255)
              cgTACGCCGCTGACCCTGTCGTGGCAGTTATCACCAGTAGCGGCCACAACCTATTCCACATACTATCCAGACTCGCATGCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGACTAGGGTTAATCCACGTCTGGTTTTCCAGCCATACCGGCTTGTTTTCCACAGGAGGCACACAACCCGAAAGGTGAAGTGCGGGCCACCTACAGACACACACAccctacaaacccccatcg                                                                                                                                                                                                                             >  1:988821/1‑223 (MQ=255)
               gTACCCCGCTGCCCCTGTGGTGGCCGTTATCACGAGTAGGGGCAAAAAATTTTTCCCCACAATACCAAGACTCCCATCCTTAAAAAAAATCTGTTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAACCCACGTAAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAAAAACGTAACCGGAAGGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCtgtt                                                                                                                                                                                                                            <  2:389140/239‑1 (MQ=255)
                           cccTTTGGTGCCCGTTTCCCCCAGTAGCGCCCACAAACTTTCCCCAACAATACCAGGCCCCGAATCTTTAAAAAAATTCTGCTTGGTAAGGCACGAAAAGGGGCAAATGGGGTTAACCCAGGTCAGGTTTTGCAGCCATCCCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACaga                                                                                                                                                                                                               <  1:1166454/239‑1 (MQ=255)
                                ccgtgccttttacccccATTACCGGCCACAAAATGTTCCACACAATACCCAGACCGGCACACTAAAAATAAACCTGCTGGTTAATGTCCGAAAAGGGGCGAATAGGGTAAATCCCCGAAGGGTTTTGCAGCCATCCGGGCATGTTTTCCACCGGAGAAACGTAACGGAAAGGGAGAAGGGGGGGCATCAAAAAAACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGtgat                                                                                                                                                                                                         <  1:670728/224‑1 (MQ=255)
                                    ggCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAAACAAACACGCCGATAAACGCCCGCTGGTGGGGTGAACaagagggttgaa                                                                                                                                                                                                      >  2:1247951/1‑228 (MQ=255)
                                     gCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCTTAAAGACAAACACGCCGCTAAAAGCCCGCCGTTGTGTTTAACAGAGGGATGAAAt                                                                                                                                                                                                    >  2:762030/1‑239 (MQ=255)
                                      ccGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGAGGCGAATAGGGTTAATCCACTTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGTGGTCATCATAAAGACAAACACCTCGATTACAGCCTCGCGTTGTGTTTGAAAagagagtaaaaac                                                                                                                                                                                                     >  2:694743/1‑230 (MQ=255)
                                        tttATCCCCATTGCCGCCCACAATTTGTTCCACACAATACCAAGACTGCATCCCTAAAAAAAATCCTGCTGGGAATGTTCCTAAAATGGGGGAATAGGGTAAACCCCCGTCAGTTTTGGAAGCCATACCGGCATTTTTCCCACGGGAAAAACTTAACGGAAAAGGAGATTGGGGGGAAACAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCaa                                                                                                                                                                                                 <  1:585749/238‑1 (MQ=255)
                                                        gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGAGCCTAAAAACAAAAACCCCGCAAAAACCCCGCTGTTGTGTTGAACAACATGATGAAAACAAAAAAACGAAAaccacc                                                                                                                                                                                 >  1:1014227/1‑239 (MQ=255)
                                                        gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAAAACGCCGATAAACGCCCGCTGTTGTGTTTAACAGCGGGGTGAAAACCACAGACCGAATCccac                                                                                                                                                                                  >  2:459423/1‑238 (MQ=255)
                                                          cacaAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATCGGGTTAATCCACGTCAGGTTTTGCAGCCAAACCGGCATGGTTTCCACCGGAGACAACTAACCGGAAAGGAGAATAGCGGGGATCGCGAAAACAAACAACCCGAAAAAAACCACCTGTTGTGTGGCAAAGAGAGATGAAAACACAAGACCGAAACCCCCCAg                                                                                                                                                                               >  1:833023/1‑239 (MQ=255)
                                                                         acaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGGATCCTAAAGACAAACACACCGATAAACGCCTGCTGTTGGTTTGAAAAAATGGATGAAATCAAAAAACCGAATCCCCCCAGCGATAAACCATaaa                                                                                                                                                                 >  2:114765/1‑238 (MQ=255)
                                                                             tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACCCCGATAAACGCCGGCTGTTGTGTTGAACAGAGTGATGAAATCAAAAGACCGAATCCCACCACCGGTAAACCATAAATCAcc                                                                                                                                                            >  2:749689/1‑239 (MQ=255)
                                                                             tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATAACGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGAATCTTAAAGACAAAACAGCCCAGAAACGCCACCTTGTGGGTTGTACAGAGGGTTGAAAACAAACAACCGAACCCCACCACCCGTAAAcacaaaaaaaca                                                                                                                                                            >  1:984217/1‑228 (MQ=255)
                                                                                 cAGACTCGCATCCTTCAAATAAATCTGTTTGGTAATGTCCGTAAAGGGGCAAATGGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATAATAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCg                                                                                                                                                        <  2:856289/239‑1 (MQ=255)
                                                                                     gtccccatgcttaaataaatCTGTTTGTGTAGTGTCCGAAAGGTGGGGTAGGAGTTTAGTCCCGGTCAGTTTTGGCAGCCATCCCGGGAGTTTTCCCCCAGAAAACCCGTCACCGAAAAGGAGATTGGCGGGCATCAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTa                                                                                                                                                      <  1:1156007/226‑1 (MQ=255)
                                                                                         cATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGCAACGTAACCGGAAAGGAGAATGGCGGGCATCCTAAAAACAAACAACACGCTAAAAGACAGCTGGTGTGTTTAACAAAAGGAGTAAAACCACAGAACGACTACCACCACAGAGAAAcacaaaataacaaacagaaaaaaa                                                                                                                                                >  1:1016939/1‑216 (MQ=255)
                                                                                         cATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGGACAACAACCCGATAAACGCCCGCTGTTTGGTTGAACAGAGGGAATAAATAAACAAACCCAAACCACCCCACGATAAAAAAAAAAACACCAACCTaaaaaaa                                                                                                                                                >  1:1188282/1‑235 (MQ=255)
                                                                                         cATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAAAACGAATCCCACCACCGATAAACCATAAATCACCATCTTaaaagag                                                                                                                                                >  2:812194/1‑235 (MQ=255)
                                                                                         cATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCCGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCCCCACCGAGAAACCAAAAATCACCCCCGTAAAgaaa                                                                                                                                                >  2:367521/1‑236 (MQ=255)
                                                                                                          tcttttttgttgtggcctaaaaGTGGCGAATAGGTTTAACCCACGGTAGTTTTTCACACCATCCCGGAATGTTTTCCACCGGAGAACCGTAACCGGAAAGGAAAATGGCGGGAAACAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGAt                                                                                                                                 <  1:932817/220‑1 (MQ=255)
                                                                                                             cTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGGTAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAAACCGAAACCCACCAGCGCTAAACCCTAAATAACCATCGTAAAGTAGAAAAGAGCCAGCGactcg                                                                                                                              >  2:520897/1‑234 (MQ=255)
                                                                                                             cTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTTAACAGAGTGATGAAATCAACAGACCCAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCgg                                                                                                                             >  2:126388/1‑239 (MQ=255)
                                                                                                                      tGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATTATAAAGACCAACAAGCCGGTAAAACCACGCTGTTGGGGTTGACAAAGGGAGTGAAATAACAAAACGAATTCCACCACCAGTAAACCAAAAAACACACCCGTCAACAAGAAAAACGCCGCCCAACCGGGGAAgggg                                                                                                                     >  1:754882/1‑239 (MQ=255)
                                                                                                                               atggggCGAAAGGGTTAAACCCCATTCGGTTTTTGCAGCCATACCGGCATTTTTTCCACCGGAAAAACGTAACGGGAAAGGAGAATGGCGGGCATAAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATa                                                                                                             <  2:984217/235‑1 (MQ=255)
                                                                                                                                    gcgatgaggttaaacccccgagaGGTTTGGCAGCAATGCGGGAATGTTTTCCACGGGAAAAACGTAACGGGAAAGGAAAAGGGCGGGCATAAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGcc                                                                                                        <  2:746306/216‑1 (MQ=255)
                                                                                                                                           gggTTATCCCACTTCAGATTTGGAGCCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATAc                                                                                                 <  2:20950/238‑1 (MQ=255)
                                                                                                                                             gTTAACCCGCGTCAGGTTTTGCAGCCATCCCGGAATGTTTTCCACCGGAAAAACGTAACCGGAAAGGAGAATGGCGGGAATCAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCa                                                                                               <  2:1014227/238‑1 (MQ=255)
                                                                                                                                                 ctccgccgaaGGTTTTGCAGCCATCCCGGCATGTTTCCCACGGGAAAATCGTAACCGGAAAGGAGAATGGCGGTCATCAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGc                                                                                          <  2:1230393/230‑1 (MQ=255)
                                                                                                                                                 acccccGTCAGGTTTGCCACCCATCCCGGCTTTTTTCCCCCGGGAAAACCGTAACCGGAAAGGATAATGGCGGGCATAAAAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGc                                                                                          <  2:1074978/234‑1 (MQ=255)
                                                                                                                                                 aTCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCCGCTGTTGTGGTGAACAGAGAGATGAAATCAACAGACCGAATCCCACCAACGGTCAACCATAAATACCAATCGTAAAGAAGAACACCGACAAGGATCCGGGGAAGGGGAGTTggaacaacaaaaaacaaacacc                                                                                           >  1:407605/1‑218 (MQ=255)
                                                                                                                                                    ccggTCAGGTTTTGCAGCCATCCCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAg                                                                                       <  2:112457/236‑1 (MQ=255)
                                                                                                                                                      ggttaGGTTTGGCGGCCATCCCGGCATGTTTTCCACCGAAAAAACGTAACCGGAAAGGAGAATGGCGGGCATAAAAAAGCAAACCACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTAGATACGCCCAGATACCAATCGCCAGca                                                                                     <  2:405762/235‑1 (MQ=255)
                                                                                                                                                      cGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGGTAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAGGGGGATTTGAAAGCCCCCGAAACCACTccccccca                                                                                     >  2:898513/1‑235 (MQ=255)
                                                                                                                                                      cGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAAACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAAAAGCGCCAACGAACCGGGGAAGGGGAATTGAAACCGCCCAAAACCAATCGCCAGcc                                                                                     >  2:434764/1‑238 (MQ=255)
                                                                                                                                                      cGTCAGGATTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGACTGCTGTTGTGTTGAACAGAAGGAGGTAATCACAAGACCGAACCCCACCCGCGATTAACCCAAAATCACCACATAAAAGAAGAAAAGACACACCGATCCCGCCCAGGGGGCTTTATAAGCCCacaaaaaaaaacccaac                                                                                      >  1:20950/1‑222 (MQ=255)
                                                                                                                                                                                        cACGGGAAAAACGAACCCGAAAAGGAGATTGGGGGGAAAAAAAAAGAAAAACACGCCGATAAACGCCTGCTGTTGGGTTGAACAGAGTGATGAAAGCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACg                                                    <  1:434764/239‑1 (MQ=255)
                                                                                                                                                                                        cACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGGGGCCTGGAAGGGGGCGACAATTaaa                                                     >  2:1094314/1‑237 (MQ=255)
                                                                                                                                                                                                        cccGAAAAGGAGAATGGCGGCAAACATAAAGACAACCTCGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGc                                     <  2:964620/238‑1 (MQ=255)
                                                                                                                                                                                                        aCCGAAAAGGAGAATGGCGGGCATAATAAAGAAAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGc                                     <  2:527361/239‑1 (MQ=255)
                                                                                                                                                                                                          cgaagaggggtaTGGGGGGCATAATAAAGAAAAACACGCCGATAAACGCCGTCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCCCCAGCGATAAACCATAAATTCCCATCGTAAAGTAGAACAGCGCCAGCTATCCGGGGAAGGGGATTTGATTCGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCg                                   <  1:204276/228‑1 (MQ=255)
                                                                                                                                                                                                             aagggAGAGTGGCGGTAATAATAAGGCACAACACCCCGTAAAACGACCGCTGTGGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGGGATAAACCATAAATCACCAGCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGAt                                 <  1:613985/235‑1 (MQ=255)
                                                                                                                                                                                                               aGGAGAATGGGGGGCATAAAAAAACAAACCACCCCGATAAACGCCTGTTGCTGTGTTGAACAGGGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGaa                              <  1:749689/239‑1 (MQ=255)
                                                                                                                                                                                                               aGGAGAATGGCGGATATAAAAAAGAAAAACACGCCGATAAACGCCTGGTGTTGTGTTGAACAGAGTGATGAAAGCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCCCAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGaa                              <  2:545955/239‑1 (MQ=255)
                                                                                                                                                                                                                                catAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGTGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGGGGCTGCAATTAACGCCGGTACGGCTTTGCCGATGAAGGTCTGCCAGGGGGGGa             >  1:1030022/1‑239 (MQ=255)
                                                                                                                                                                                                                                    aaagcaaaccacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAgcg          <  2:754882/230‑1 (MQ=255)
                                                                                                                                                                                                                                          aaCACGCCGATAAACGCCTGCTGTTGGGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACCCCAAGATCCCAACCCCCAGCACCATGGGCGCCTGGAAGGGCGCGCGATTTACACCCGGAACGGGCTTCCCGTGGAATATCGGCAAGGGGGGGAGCGGGGaaa  >  1:1033805/1‑238 (MQ=255)
                                                                                                                                                                                                                                           accacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAAc   <  2:1279645/237‑1 (MQ=255)
                                                                                                                                                                                                                                           accacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTACCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAAc   <  2:749693/237‑1 (MQ=255)
                                                                                                                                                                                                                                           acacGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCCCAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGGGAAAcc  >  2:844732/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
ACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAAGACAAACACGCCGATAAACGCCTGCTGTTGTGTTGAACAGAGTGATGAAATCAACAGACCGAATCCCACCAGCGATAAACCATAAATCACCATCGTAAAGTAGAACAGCGCCAGCGATCCGGCGAAGGGGATTTGATACGCCCAGATACCAATCGCCAGCACAATGGTGGCCTGGAAGGTGGCGACAATTAACGCCGGTACGGCTTTGCCGATGAAGATCTGCCAGGTGGTGAGCGGCGAAACC  >  NC_000913/825014‑825482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: