Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 861,108 A→C 16.7% V500G (GTG→GGG)  ybiW ← putative pyruvate formate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913861,1080AC16.7% 226.6 / 4.8 97V500G (GTG→GGG) ybiWputative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (48/28);  new base C (7/13);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 4.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCACTGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAAT  >  NC_000913/860876‑861337
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
cttccccgcccgcaagttGTTGCCGGAGTTGGACATGCCGACCTGCAGGCCAAAAACCCAGTCAAATTGCGCGCCGCCTTGCTTGATTCTTTTCGCTCGCTCAATTCAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGAACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCAt                                                                                                                                                                                                                                   <  1:799703/222‑1 (MQ=255)
 ttcACTGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGCCATATTCGATTTCGATTGATTTGCGGGGGTAGTAACGGATTTCGGGATCCCACCCGTCCATCCCTTCCTc                                                                                                                                                                                                                                  >  1:353572/1‑239 (MQ=255)
  tcACTGCCGCCAGGCTGTTGCCGAGGTAGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTGGCGCCGCCTTGCTTTATACTTTTCGCTCGCTCAATACAGTCATCCACCACCGCCGAGCAGAGACTATCGTGCACGATCTCTTCCAGCAGGGGGTCGACGCCATATTCGATTTCGATTGATTTGCGGCGGTAGTAAAGGATTTGCGGATCCCACGCGTCCATCACTTCATCg                                                                                                                                                                                                                                 >  1:648057/1‑239 (MQ=255)
   ccctcccgccTGGTTGTTGCCGGTGTTGGCCATTCCATACTGCAGGTCAGAAACCCAGTCATATTGTGCGCCGCCTTACTGAAGTATTGTCGGTCGCGCAATTCAGTCATCCACCAGCGCGGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGGAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGa                                                                                                                                                                                                                                <  1:982493/234‑1 (MQ=255)
   cACTGCCGGCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGa                                                                                                                                                                                                                                <  2:747111/239‑1 (MQ=255)
      tGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATTTCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAgt                                                                                                                                                                                                                             >  2:695346/1‑239 (MQ=255)
        ccgccAGGCTGTTGCCGAGGTTTGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGATCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAgttg                                                                                                                                                                                                                           <  2:1229926/239‑1 (MQ=255)
         cgccAGGCTGTTGCCGAGGTTGGCATTGCCGACCCTCACGCCAGAACCCCAGTTCTATTTCGCGCCGCCGTGCTTGATACTTTTCTCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAgttgt                                                                                                                                                                                                                          <  2:88960/239‑1 (MQ=255)
             aGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGCCGACATATTCGATTTCGATTGCTTTGCGGGTGTAGGAACGGACTTGGGTTTCCCACTCGTCCATCATTTCACCGAGATTGTTaaa                                                                                                                                                                                                                      >  1:512476/1‑239 (MQ=255)
                  gTTGCCGAGGGTGGCAAGACCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCCCGTTGTCTTCCAGCATGGGGTCGCCGACATATTCGCTTTGTATTGATTGGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCCCTTCagacgagtagttgagctaac                                                                                                                                                                                                                 >  1:910564/1‑220 (MQ=255)
                   ttGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGCTTTCGATTGATTTGCGGGAGGAGTAAAGGATTTGCGGATCCCACCCGGCCCTCCCTCCATGGAAATTGTGGAAGTTAcc                                                                                                                                                                                                                >  1:1069302/1‑239 (MQ=255)
                   ttGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTa                                                                                                                                                                                                                  >  2:533395/1‑237 (MQ=255)
                   ttGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGGAACGGATTTGCGAATCCCACGCTTCCATCCCTTCTTCGAAGTTGTTGAAGTTa                                                                                                                                                                                                                  >  2:955922/1‑237 (MQ=255)
                             tGGCCATGCCCCCCTGCAGGCCAAAAACCCAGTCATTTTTCGCCCCGCCTTGCTTGATTCTTTTCGCCCGCTCAATACAGTCCTCCACCAGCGCCGAGCAGAGATTTTCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTAGCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAAc                                                                                                                                                                                                      <  1:909429/239‑1 (MQ=255)
                             tGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGCCGACATATTCGATTTCGATTGATTTGCGGGGGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCACAGTTTTTGAAGTTACCTGCCgcaaa                                                                                                                                                                                                       >  1:313419/1‑234 (MQ=255)
                                  aTGCCTCCCTGTAGCCCAGAAACCCAGTCATTTCTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCGCAATACAGTCATCCACGTGCGCCGAGCATAGAATATCGTGCCCGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAATTTACCTGCCGACAACGCtt                                                                                                                                                                                                  <  1:592269/238‑1 (MQ=255)
                                   tGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTGGTAAAGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAGGTGGTTTAAGTTAACTGCCGCCAAGGCtttt                                                                                                                                                                                                >  2:614933/1‑239 (MQ=255)
                                   tGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCAACGCGTCCATCCCTTCATCGAAGTTGTTTAAGTTACCTGCCGCAAACGCtttt                                                                                                                                                                                                >  2:412197/1‑239 (MQ=255)
                                     ccGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCtttt                                                                                                                                                                                                >  2:101333/1‑237 (MQ=255)
                                        aCCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTtg                                                                                                                                                                                           >  2:499547/1‑239 (MQ=255)
                                        aCCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTCTCGACGACATATTCGATTTCGATTGATTTGCGGGTGCAGTAACGGACTTGCGTATCCCACGCGTCCATCCCTTCTTCGAGGTTGTTTAAGTTCCTTGCCGACAAACCtttttttt                                                                                                                                                                                            >  1:1284871/1‑238 (MQ=255)
                                         ccTGCAGGCCAGAACCCCAGTCATATTTCGCGCCGCCTTGCTTGATTCTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTtgt                                                                                                                                                                                          <  2:691772/239‑1 (MQ=255)
                                         ccTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGGGTAGTAACGGATTTGCGAATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCACCAACGCTTTTTCTtgt                                                                                                                                                                                          >  2:727682/1‑239 (MQ=255)
                                          cggCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTtgt                                                                                                                                                                                          <  2:353572/236‑1 (MQ=255)
                                           ttctggCTAGAACCCCAGTCTTTTTTCGCGCCGCGTGGCTGGATTCTTTCCGCTCTCTCAATACCGGCATCCCCCGGGGCCGAGCAGGGAATATCGTGCACGTTCTCTTCCAGCATGGGGGCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTgg                                                                                                                                                                                        <  1:70480/235‑1 (MQ=255)
                                                     aaaCCCCGCCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCCCCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGCCATATTCGCTTTCGATTGATTTGCGGGGTTCGTAACGGATTTGCGTATCCCACGCGTCCATCCCTTCATCGAAGTGTTTGAAGTTACTTGCCGACAACGCTTTTTCTTGGGGCAGGaaacct                                                                                                                                                                             >  1:810488/1‑235 (MQ=255)
                                                          cAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGCTTTGCGTATCCCACGCGTCCATCACTTCATCGACGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGTCGGAAACCttttc                                                                                                                                                                         >  2:190286/1‑236 (MQ=255)
                                                            gTCATGTTTCGCGCCGCCTTGCTTGATTCTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGc                                                                                                                                                                      <  2:1156654/239‑1 (MQ=255)
                                                            gTCATATTTCGCGCCGCCTTGCTTGATTCTTTTCGCCCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGc                                                                                                                                                                      <  2:905965/239‑1 (MQ=255)
                                                                  tttCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACAATTTGCCGCtggtg                                                                                                                                                                >  2:176844/1‑238 (MQ=255)
                                                                  tttCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTCCCGACAACGCTTTTTCTTTGGGCAGGGAAACTTTTCCGCcggtgg                                                                                                                                                               >  1:820784/1‑239 (MQ=255)
                                                                      gggccgccTTGCTTTATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCTCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGcatc                                                                                                                                                           <  2:461174/237‑1 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCCTGGGGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCCTCACTTCATCGAGCTTGTTCAAGTTACCTGCCGACAACCCTTTTTCTTGTGGCAGGAAAATCTTCCCGCCGGGGGcatca                                                                                                                                                          >  1:1214957/1‑239 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGcatca                                                                                                                                                          >  2:1288371/1‑239 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAAAACTTTTCGGCTGGTGGcatc                                                                                                                                                           >  2:496600/1‑238 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTCCCGACAACCCTTTTCCTTGGGGCAggtaacctatgcctcgtctggacac                                                                                                                                                            >  1:461174/1‑215 (MQ=255)
                                                                           gccTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGGGGCATCATGc                                                                                                                                                       >  2:1177339/1‑238 (MQ=255)
                                                                           gccTTGCTTGATACTTTTCCCTCGCTCAATGCAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCTCGCGTCCATCCCTTCATCGAAGTTTTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGcc                                                                                                                                                      <  2:182730/239‑1 (MQ=255)
                                                                            ccTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGCGTCGACGACATATTCGATTTCGATTGATTTGCGGGCGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTGTGGCAGGAACACCTTCCCCCCGGTTCCATCCTGcc                                                                                                                                                      >  2:987019/1‑238 (MQ=255)
                                                                             cttgcttgATTCCTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTTTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCg                                                                                                                                                    <  2:1081527/239‑1 (MQ=255)
                                                                                      tttttgtcgcttgtTCAATGCAGTGCGCCCCCAGCGCCGAGCAAAGGATATCGTGCACGTTCTCTTCCAGCGTGGTGTCGCGGACATATTCGATTTCGTTTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTCACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACGCTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAgc                                                                                                                                           <  1:496600/233‑1 (MQ=255)
                                                                                      tACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTc                                                                                                                                                                                              >  2:1111424/1‑190 (MQ=255)
                                                                                           ttCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTTTGGCAGGAACACTTTTCCGCTGGTGGCATCATGCCCGCCTTCCCGcgccg                                                                                                                                       >  2:1200047/1‑238 (MQ=255)
                                                                                           ttCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGCATCCCACGCGTCCATCACTTCATCGCAGTTGTTGAAGTTACCTGCCGTCAACGCTTTTTCTTGTGGCAGGAACACTTTGCACCCGGTGGCATACTGCCCGCCTTCCAccgccg                                                                                                                                       >  2:97629/1‑238 (MQ=255)
                                                                                           ttCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGCGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTATTGAAGTTACCTCCCGACAACGCTTTTTCTTTTGGCAGGACAACTTTTCCGCTCGGGGGCACAAGCCCCCCTTACAgccgcca                                                                                                                                      >  1:23131/1‑234 (MQ=255)
                                                                                           ttCGCCCGCTAAAGACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGcgccgc                                                                                                                                      <  2:1069302/239‑1 (MQ=255)
                                                                                                tcgctcAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTGTGGCAGGAAACCCTTGCCGCTGGTGGCATCCTGCCCCCCTTCCAGCGCCCCCacc                                                                                                                                  >  2:315745/1‑236 (MQ=255)
                                                                                                   ctcAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATTCCCGCCTTCCAACGCCGCCAGCATc                                                                                                                               >  2:951211/1‑238 (MQ=255)
                                                                                                     caattacgTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCAcg                                                                                                                            <  2:199922/232‑1 (MQ=255)
                                                                                                             tCACCCCCCGGCGCCGGGCTGAATATCTTGTCCCCGTTTTTTTCCATCTTGGTGTGGATGACAGACTCAATTTCGATTGATTTGCGGGTGAAGTAACTGATTTGCGTACCCCACCCTTCCATCCCTTCATCGAAGTTTTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGaa                                                                                                                    <  1:1200047/239‑1 (MQ=255)
                                                                                                              cagccaccaGCGCCGAGCATAGAATTTCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCAGCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGc                                                                                                                       <  2:931160/232‑1 (MQ=255)
                                                                                                              cATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTTCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACCCGGGCGAAg                                                                                                                   >  2:180289/1‑239 (MQ=255)
                                                                                                                  caccaGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTACCGGATTTGCGTATCCCACGCGTCCATCACTTCATCGACGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTGTGGCATGAACACTTTCACCCTGGTGGCCCAACTCCCCCCTTCCCGCGCCCCCCGCATCACCCGGGCggcgctgt                                                                                                               >  1:992694/1‑232 (MQ=255)
                                                                                                                    ccgaccgcgggCAGAGAATTTCGGTCGCGTTTTTTTCCAGCAGGGTGTCGGCGACATTGTCGATTTCGATTGATGTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATa                                                                                                             <  2:810488/229‑1 (MQ=255)
                                                                                                                       gcgcCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATTACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTTCCGCTGGTGGCATCATTCCCGCCTTCCAGCGCCGCCACCATCACCCGGGCGAAGTTGTTaaa                                                                                                           >  2:1243971/1‑238 (MQ=255)
                                                                                                                       gcgcCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTTCCGCCGGTGGCATAATGCCCGCCTTCCAGCGCCGCCAGCATCACGCCGGCGCAGTTGGTaaa                                                                                                           >  2:965860/1‑238 (MQ=255)
                                                                                                                       gcgcCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCCCCTTCCACGGCCGCCCGCCTCACGCGGGGGGAGCTGGTAAAg                                                                                                          >  2:154065/1‑239 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTTCACGTTCTCTTCCAGCAGGGTGGCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGc                                                                                                                       <  2:8478/220‑1 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTACCGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTTTGGCAGCGAAAACTTGCCGCTGGTGGCAGCAGTACCGCCTTCCTCCCCCCCCCCACTCACCCGGGCGAGCCTGATAAAGCCCac                                                                                                     >  1:1242469/1‑237 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCCCTGGTGGCATCAAGCCCGCCTTCCAGCGCCCCCCGCATCCCCCGGGCCAAGTTGTTAAAgtccat                                                                                                     >  2:177614/1‑233 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAAAACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGCAGTTGATAAAGCCCAt                                                                                                     >  2:1002649/1‑238 (MQ=255)
                                                                                                                               cAGTGAATTTCGTGCCCGTTTTCTTCAGGCGTGGGTACGAGAACATTTTCTGTTTCGATTGATTTGGGGGTGTAGTAACGGATTTGCGTTCCCAACGCGTCCATCCCTTCATCGAAGTTGTTGAATTTCCCTGCCGACACCGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGc                                                                                                   <  1:176844/238‑1 (MQ=255)
                                                                                                                                   ttcttttgttctttttttctTCCAGCGTGGTGTCGCTTCATTTTTTGATGTCGATTGATTTGCGGGTGTAGTAACGTAGTTGCGTGTCCCACGCGTCCATCACTTCATCGAGGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCATGAACACTTTGCCGCGGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTa                                                                                              <  1:499547/222‑1 (MQ=255)
                                                                                                                                    aaTATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGGAAACTTTTCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTTGTAAACATCCTGCCGGtgc                                                                                             >  2:206458/1‑237 (MQ=255)
                                                                                                                                     atttCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTAc                                                                                             <  2:1141006/235‑1 (MQ=255)
                                                                                                                                     atatGGTGCACGTTCTCTTCCAGCATGGTGTCGACACCATTTTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTTTCCCACGCTTCCATCCCTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACa                                                                                            <  2:308006/239‑1 (MQ=255)
                                                                                                                                            gCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCCGCATCACGCGGGCGAAGTTGATAAAACTCCTGCCGGTCCACCGCTa                                                                                      >  2:775148/1‑238 (MQ=255)
                                                                                                                                            gCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGGGGCATCATTCCCGCCTTCCAGCGCCGCCAGCCTCACCCGGGGGAAGATGTTAAAGCCCATTCCGGTACAGCGaaa                                                                                      >  2:745601/1‑236 (MQ=255)
                                                                                                                                                      ttCCAGAATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCt                                                                                                        <  2:774974/210‑1 (MQ=255)
                                                                                                                                                            cATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACCCTTTTCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACCCGGGGGAAGTTGATAAAGCTCAATCCGGTACAGCGATAGCCCCCTTTGCAACCg                                                                      >  2:1241528/1‑238 (MQ=255)
                                                                                                                                                            cATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAAAACTTTTCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACCCGGGCGAAGTTGGTAAAGGTCATTCCGGTCAAGCGCAAGCCCCCTTTTCCAcccc                                                                     >  2:758758/1‑237 (MQ=255)
                                                                                                                                                                gtgtCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTTTGGCAGGGACCAGTTTCGGCTGGTGGTCTCATGCTCCCCTTTCACGGCCGCCACGCTGCCCCGGGCGAAGTTTGAAAAACTAAATCCGGTCCACCGAAAACCCCATCTGCCAcacaacgg                                                                 >  1:298749/1‑232 (MQ=255)
                                                                                                                                                                   tCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTACCGGATTTGCGTATCCCACGCTTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACCCTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGt                                                              <  2:46018/239‑1 (MQ=255)
                                                                                                                                                                    ttctatcatttttgagtttctattTGTTTGCGAGTGTCGTTACGTATTTTCTTCTCCTACGCGTCCATGTCGTCATCGAATGTTTTGAATTTCCCTGCCTACAACTCTTTTTCTTGGGACAGGAACTCTTTGCCGCGGGTGGCCTCACGCCCGCCTTCCGGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATATCCCCATTTGCCACCGACGGCGGt                                                              <  1:466835/223‑1 (MQ=255)
                                                                                                                                                                    ggactctatatTTGATGTTTGATTTTTTTGCGGGTGATGTATCGGGTTGGCGTCACCCACGTGTCCGGCCATTCATCGAAGTTGGTGAACTTGGCTCCTCACAACGCTGTTTCTTGTGGCAGGAACTCTTTGACGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGt                                                              <  1:534103/232‑1 (MQ=255)
                                                                                                                                                                    cgacgaCATATTGGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGtt                                                             <  2:1284871/239‑1 (MQ=255)
                                                                                                                                                                          cgatttctctttttctgtttattTGGGGGTGTAGTAACGGATTTGCGTCTCCCTGGCGTCATACCCTTCAGCTAGTTTGTGTAAGTTCCCTGCCGACAACGCTTTTTCTGGTGGCAGGAACACTTTGCCGCGGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCta                                                         <  1:177614/219‑1 (MQ=255)
                                                                                                                                                                           atatTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGTAACACTTTGCCGCTGGAGGCAGCTAGCCCCCCCTCCAGCCCCCCCCGCTCCCCCCGGGGCAGGTTTGGAAAGCCACTGCCGCTACAACGATAGCCCCCATTGACCCCCAcggcggggttaaaaa                                                      >  1:646616/1‑230 (MQ=255)
                                                                                                                                                                                        cgattGTTTTGGGGGTGTAGTACCGGATTTGCGTATCCCACGCGTCCATCCTTTCATCGAAGTTGTTGAAGTTACCGGCCGACAACGCTTTTTCTTGTGGCAGGACCACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCt                                           <  2:69400/239‑1 (MQ=255)
                                                                                                                                                                                          gttgattTGCGGGTGTAGTAAGGGATTTGCGTATCCCCCCCTTCCATCCCTTCATCGAATTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGc                                         <  2:992694/238‑1 (MQ=255)
                                                                                                                                                                                          gttgattTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCCCTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGc                                         <  2:294850/238‑1 (MQ=255)
                                                                                                                                                                                          attgattTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCCCCTTCCAGCGCCGCCAGCATCACGCGGGCCAAGTTGTTAAAGCTCATGCCGGTACAGCGATAGCCCCATTTTCCACCGCCGGCGGGTTTTTTCCAACCCATCgacgc                                         >  2:55481/1‑235 (MQ=255)
                                                                                                                                                                                           ttggttTGGGGGTGTAGTAACGGATTTCGGTATCCCAAGGGTCCATCCTTTCATCGAAGTTGTTGAAGTTCCCTGCCGACAACGCTTTTTCTTGTGGCAGGAACCCTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCg                                        <  2:586121/239‑1 (MQ=255)
                                                                                                                                                                                           ttgattTGCGGGGGTAGTAACGGATTTGCGTCTCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGAAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGc                                         <  2:313419/238‑1 (MQ=255)
                                                                                                                                                                                            tggtttGGGGGTGTTGTAACGGATTTGCGTATCCCACGGGTCCATCACGTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGt                                       <  2:893125/236‑1 (MQ=255)
                                                                                                                                                                                                        gtagtaACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGGTAGCCCCATTTGCCACCGACGGCGGGTTTTATACAACCAATCGCTGCGTAGTCATAAGCg                            >  2:394319/1‑238 (MQ=255)
                                                                                                                                                                                                            tacctgttttGCTTCTCCCACCCGCTCCTCCCTTAATGGGAGTTTTTGAAGTTGCCGGCCGACACCGCTTTTTCTTGGGGCAGGAACCTTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTg                       <  1:180289/232‑1 (MQ=255)
                                                                                                                                                                                                            taACGGATTTGCGTATCCCACGCGTCCATCCCTTTATCGAAGTTGTTGAAGTTACCTGCCGACAACGTTTTTTCTTGGGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTg                       <  2:648057/239‑1 (MQ=255)
                                                                                                                                                                                                              aCGGATTTGCGTTTCCCACGCGTCCATCCTTTCATCGAAGTTGTTGAAGTTCCCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCg                     <  2:1253869/239‑1 (MQ=255)
                                                                                                                                                                                                               gatgggtaaaagagacaggcgTCCATAACTTCATCGAAGTTTGTGAAGTTCCCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTGGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCg                     <  2:1267935/220‑1 (MQ=255)
                                                                                                                                                                                                                 gATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTTCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCCTCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCCTTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGGCCTAAACGTCCTGCGGtt                  >  2:247395/1‑239 (MQ=255)
                                                                                                                                                                                                                   tttGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCCTTTGCCACCGACGGCGGTTTCTATACAACCAATCCCCGCGTAGCCATAAACGTCCTGCGGTTCa                >  2:481339/1‑239 (MQ=255)
                                                                                                                                                                                                                    ttGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCCCCGACGGCGGGTTTTTTACAACCAAACCCTGCGTAGGCATAAACGTCCTGCGGTTCaa               >  2:901673/1‑239 (MQ=255)
                                                                                                                                                                                                                     tGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAACTCATGCCGGTACAGCGATAACCCCAATTGCCACCGACGGCGGGTTCTTTTCAACCAATCGCTCCGACGTCATAAAGGTCCTCCGGTTCata              >  2:641298/1‑237 (MQ=255)
                                                                                                                                                                                                                      gCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGGTTCTTTACAACCAATCGCTGCGTAGTCATAAACGGCCTGCGGTTCAAt              >  2:358875/1‑238 (MQ=255)
                                                                                                                                                                                                                            cccACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCCGCGCCGCCAGCATCACGCGGGCGAAGTTGTTAAAACTCCTTCCGGTCACGCGGTCACCCCATTTGCCACCGACGGCGGGTTCCATATCACCACACGCCGCGAAGACCCAAACGCCCTCCGGTTGAAAAccacgt       >  1:1272587/1‑235 (MQ=255)
                                                                                                                                                                                                                                 gcgTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGGGGCCTCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGACGTTGATAAAACTCATGCCCGGACCGCGATAACCCCATTTGCCCCCGGCCGGGGGTTCTTTTCAACCAAACGGCGCCGAGTCAAACACGTCCGCCGGGTCCATAACGAgggtaaa  >  1:248747/1‑233 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
CTTCACTGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAAT  >  NC_000913/860876‑861337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: